développement de méthodes rapides pour l'authentification des produits biologiques
DESCRIPTION
Kristian HOLST-LAURSEN, de la Faculté des sciences, Section plantes & sol, de l'Université de Copenhague (Danemark), revient sur les méthodes rapides pour l'authentification des produits, en s'arrêtant sur le projet Européen "AuthenticFood".TRANSCRIPT
Sûreté alimentaireQuelles innovations pour
la maîtrise des contaminants et l'authentification des produits
agricoles et alimentaires ?
> 13 & 14 novembre 2013 > Montpellier SupAgro INRA
www.rencontres-qualimediterranee.fr
Kristian HOLST LAURSEN
Faculté des sciences, Section plantes & sol,
Université de Copenhague (Danemark)
Fast methods for authentication of organic basedfood
- presentation of the "AuthenticFood" project
Laursen, Kristian Holst, Dr., University of Copenhagen
Organic versus conventional plant production
Laursen, Kristian Holst, Dr., University of Copenhagen
Organic Conventional
Pesticides No Yes
Synthetic fertilizers No Yes
Organic fertilizers Yes Yes
Fertilizer amount Lower or similar Higher or similar
Crop rotations Longer Shorter
Yields Lower or similar Higher or similar
Product price Higher Lower
Organic agricultural land in Europe
Laursen, Kristian Holst, Dr., University of Copenhagen
Source: www.fibl.org
Credibility in the organic market- Certifiction does not protect consumers
Laursen, Kristian Holst, Dr., University of Copenhagen
Research objectives of AuthenticFoodTo develop and test a portfolio of analytical techniques forauthentication of organic plant foods
• Ionomics• Stable isotopes• Metabolomics• Pesticide screening
To evaluate the techniques in corporation with Europeaninspection and certification bodies
• Ecocert - France• BIOS - Italy• DVFA - Denmark
Laursen, Kristian Holst, Dr., University of Copenhagen
AuthenticFood partners
Laursen, Kristian Holst, Dr., University of Copenhagen
KU‐ScienceFOOD‐DTUDVFA
FERA
AIABFEM‐IASMABIOS
EurofinsEcocert
Bioforsk UHEL
RIKILT
ICT
GL Centre Unika
LIAEFP7 ERA‐NET project16 partners11 European countriesProject leader: Søren HustedPeriod: 2011‐2014
AuthenticFood workpackages
WP1: Project coordinationWP2: Sample production and distributionWP3: Multi-elemental analysis, 31P (NMR)WP4: Stable isotopes, δ2H, δ13C, δ15N, δ18O, δ34S, nitrate
and sulfate isotopes, δ15N in amino acids WP5: Metabolomics, phenolic content, volatile analysis, fatty
acids, sensory profilesWP6: Pesticide residuesWP7: ChemometricsWP8: Results dissemination
Laursen, Kristian Holst, Dr., University of Copenhagen
Biological cases
Laursen, Kristian Holst, Dr., University of Copenhagen
Experimental factors• Organic/conventional• Plant species• Plant variety• Growth season• Geography• Processing
AuthenticFood project status
Laursen, Kristian Holst, Dr., University of Copenhagen
Essential plant nutrients
Laursen, Kristian Holst, Dr., University of Copenhagen
The periodic table
H
He
Li
Be B
C
N
O
F
Ne
Na
Mg Al
Si
P
S
Cl
Ar
K
Ca
Sc
Ti
V
Cr
Mn
Fe
Co
Ni
Cu
Zn
Ga
Ge
As
Se
Br
Kr
Rb
Sr
Y
Zr
Nb
Mo
Tc
Ru
Rh
Pd
Ag
Cd
In
Sn
Sb
Te
I
Xe
Cs
Ba
La
Hf
Ta
W
Re
Os
Ir
Pt
Au
Hg
Tl
Pb
Bi
Po
At
Rn
Fr
Ra
Ac
Rf
Db
Sg
Bh
Hs
Mt
Uun
Uuu
Uub
Lanthanider Ce
Pr
Nd
Pm
Sm
Eu
Gd
Tb
Dy
Ho
Er
Tm
Yb
Lu
Actinider Th
Pa
U
Np
Pu
Am
Cm
Bk
Cf
Es
Fm
Md
No
Lr
Non‐mineral Macronutrient Micronutrient
Basically all trace elements are found in plants – The Plant Nano-nutrients
Laursen, Kristian Holst, Dr., University of Copenhagen
Mass (m/z)
0 50 100 150 200 250
Ion
inte
nsity
(cou
nts
s-1)
1e-1
1e+0
1e+1
1e+2
1e+3
1e+4
1e+5
1e+6
1e+7
1e+8
1e+9
1e+10
Ni reference line
B
Mg K
Mo
Mn
Essential Nutrients (11B - 95Mo)Background subtracted spectrum
Mass (m/z)
0 50 100 150 200 250
Ion
inte
nsity
(cou
nts
s-1)
1e-1
1e+0
1e+1
1e+2
1e+3
1e+4
1e+5
1e+6
1e+7
1e+8
1e+9
1e+10
Co
Cd
Se
Mass scan 7Li – 238U
Multi-elemental plant tissue analysis
Laursen, Kristian Holst, Dr., University of Copenhagen
Stable isotope analysis
Laursen, Kristian Holst, Dr., University of Copenhagen
99.63% 0.37%
”light” isotope ”heavy” isotope
14N 15N
10001
14
15
14
15
15
air
air
sample
sample
sample
NN
NN
N
Units o/oo
Multi-isotopic analysis can reveal the fertilization history of plants
Laursen, Kristian Holst, Dr., University of Copenhagen
δ15N (bulk tissue)
δ18O (nitrate)
Low δ15N • Synthetic• Legume
High δ15N • Slurry• Manure• Compost
Low δ18O• Legume
High δ18O• Synthetic
How was I fertilized?
Conclusions
Laursen, Kristian Holst, Dr., University of Copenhagen
Metabolites
Trace elements
Pesticide residues
Compund‐specificisotopes
Stable isotopes
Laursen, Kristian Holst, Dr., University of Copenhagen
Thank you for your attention!
www.coreorganic2.org/authenticfood
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