determining ruminant materials in feed ingredients larry l. whitlock, sr. ph. d. office of the texas...
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Determining Ruminant Materials In Feed
IngredientsLarry L. Whitlock, Sr. Ph. D.
Office of The Texas State Chemist
College Station, Texas 77841
ASFFPCO Annual Meeting
June 22-25, 2003
Fort Worth, Texas
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Microscopy Equipment
• Compound Microscope
• Steroscopic Microscope
• Reagents for Spot Tests
• Accessories
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Microscopic Procedures
• Use a 20 gram Sample for Viewing with the Stereomicroscope
• Take a Separate 10 gram Sample for Floatation
• Observe Mineral Portion
• Spot Tests for Confirmation
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Spot Testing Reagants
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Examples of Spot Testing• 10% HC1 for Carbonates, Limestone, or
Oyster Shell• Nesslers Reagent for Ammonium Containing
Compounds• Urea Spot Testing• Silver Nitrate for Chlorides• Quimociac for Phosphates
NOTE:Work with all floatation procedures in a hood to prevent breathing the vapors from the solvent
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Application of Microscopy
• Levels of Meat and Bone in Feed Sample
• Identify Milk and Dried Blood
• Microscopy is a Rapid Procedure
• OTSC Current Surveys
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Meat and Bone in Feed
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Meat and Bone Meal in Feed
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Meat and Bone Meal in Feed
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Meat and Bone Meal in Feed
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Bone Fragment
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Animal Bone in Feed
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Blood Meal in Feed
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Meat and Bone Meal
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Poultry Meal
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Feather Meal
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Hydrolyzed Feathers
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Agri-Screen by Neogen Corporation
• Neogen has Two Test Strips
• Presence of Ruminant Meat and Bone in Animal Protein Products
• Presence of Ruminant Meat and Bone in Feeds
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Agri-Screen Procedures for Ruminant M&B Meal
• Weigh 10 grams Sample in Mason Type Jar• Add 100 ml Extraction Solution• Place in Boiling Water Bath for 10 Minutes• Remove Mason Jar from Water Bath• Prepare Sample Tubes and Transfer 0.5 ml of
Extract to a Tube (Use different pipette for each sample)
• Place Sample Strip into each Sample Tube• Develop for 10 Minutes and Read Results
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Reading Strip Results
• Negative-Control Line Appears Only
• Postive-Control Line and Sample Line Appear
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