Determination of some physiological parameters in Awassi Ewes in Iesponse toVitE and Se injections

AAziz Makkawi I Amera Hamra and Saleh Badie EI Abdalla2

Abstract

Thirty Awassi Ewes Aged between 3-35 year with body weight of 50 plusmn 127 kg were used to study some physiological effects of inj ting ViLE and VitE+ selenium (Se) Thnsc animals were divided into three equal experimental groups Animals in group (T I ) vere given VitE group (T2) were given VilE+Se hile group (C) were kept a a control

ViLE amp Sc were given intramuscu larl y for 8 weeks (4 before mating and 4- aft r mating) Fstrus was synchronized by sponges impregnated with Progesterone Calculations o f some blood parameters (WBe DitTerential WBC and PVC) were detemlined

Data on estrus synchroni7ation conception rate ~ rtility litter size and lamb birth veight were analyzed

A significant (PltOO I) increase in WBC and lymphocytes were observed in both (T2) amp (T ) compared to (C) group while the neutrophils decreased signiticantly (PltOO J) in both treatments compared to thc(C ) group

Little effect on monocyte and no effect on basophile eosnophil while a signi ficant (Plt O I) increase in (PVC) were observed in (T2) amp (1] ) compared to (C) group

Con(erning the reproductive perfonnance conception rate fertility and iller SiLt wert increased significantly (PltOO I) in both treated groups compared to the (C) group ambs body weight (l ) showed a significant (PltOOI) increase compared to (T2) and (C) group

In conclusion this study tends to support the positive response of ViLE and Se supplementation for improving some physiological performance of Jwassi Ewes

1- Dept Of animal Science College of Agric Studies Sudan University of Science and TechnoloiO udan 2- Dept of Animal Production Ministry of Agriculture Syria

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r

Introduction

Many new techniques have been developed during Lhc last decadls Thcst lechniques ere

approached to obtain the highest improvement of reproductive tniit of animals Arti licial

insemination and hormonal treatmcnts super ovulation and embryo transfcr (Bearden and

Puguay 1917) w~n laruple~ uf these new Lellmiques UtaL have been applied to Improve

rcproductive performance Also new studies have been referred to use one of thc metabolic

signals to stimulate gonadotrophic hormonal release (Hall et al 1992) Speci fie nutrients that are

involved in r production (Martin and Wa lkcd-Brown 1995) growth and development (Lindsay

et al 1993 Fosler 1994) may be used for the same goal

Previous reports have suggested that vitamin E and elcllium (Se) are one of the important

nutrients that can arfect mallY biological processes in the body such as immunity (Hemken et a1

1998 ) Metabolism (Awadeh el ai 1998) and reproduction (Jen) 1996) in addition ViC E and

Se improved spermatogenesis and senien middot quality (Marin-Guzman 1990 Brzezinsksshy

SI -bodzinska ai 199-)

Therefore this study was conducted to evaluate the effect ofVitF and combination of ViLE and

Se on me physiological parameters in Awassi Ewes

Materia ls and Metbods

Thirty Awa si-breed ewes aged between 3 - 35 years with body weight of 50plusmn 127 kg Wlrc

us d in thi s study to determine the effect of injecting Vit E and ornbination of Vit + Selenium

(S) on some blood parameters ego Whi te blood count (WBC) Different WBC Packed Cell

Volume (reV) and I ymphocytes and reproductive performance conception rate feriility and

liLLer ~izc

The e peri mental animals were divided into three equal groups (10) in each group Animals in

group (f 1) wen given Vit - group (T2) were given Vit E + Sc whik group (C) wcre k~pl 1I U

control

Jluc healthy and sound rams aged between (2 5 - 30) yems with all a erage weight or 0 shy

076 kg Vere used for natural mating those experimental ewes at a rate of one ram for (ach ten

ewes ( I 10)

2

All the experimental animals were hou cd in s mi closed bams designed a cording to the

prevai ling type in the area and the tropics Free access to drinking water restricted

conccntrate

(Table I) fed at a rate of hal f kg per head per day and free natural grazing was the pattern or reeding those experimental animals Vito and se were given intramuscularly (or R weeks (4

befon mating and 4 after mating) estrus was synchronized by sponges impregnated with

Progesteronc (containing 40 mg Mcdoxy Progesterone Inter Vet - Holland)

Calculations of the bl od parameters (WBC Deferential WBC and peV) were determined by

llsing Automated Hematological Analyses - known as (SYSMEX)

For the reprodu lion perfomlance synchronized estrus was determined by introducing

vasectomized rams twice a day at early morning and early evening Then thc conception rate

fertili ty litter si7e and lamb birth weight were observed and recorded for analysis

Statistical Analysis

The rccorded data was then collected for stati tical analysis using (Sat Soft Programme 1995)

and (ANOY A) as analytical technique and (L D) was uscd to determine the di rfClcncl between

the means

3

Ration Ingredients age Crude proteiI

Crude Protein 0

in Ration

Barley 60 9 54

Wheat Bran 185 14 middot259

Cotton Seed Cake 20 22 44

Calciul1l I - -Sodium Ch loride 05 - -

100 45 1239

Results and D iscussion

The effect ofVit E (T ) and V it E+Se (T2) on some blood parameters are shown in (Table

2) Results showed that supplementation of Vit E and Se increased significantly (PltO05)

the total number of WBC count in comparison to control group (C) WBC count were

8180 12360 and 16200 in C T I and T2 groups respectively This may be due to the

improvement or immunological function of the body (Meydani 1990) WBe count is an

important tool for diagnos is of the health conditions and problems and may undergo to

many physiological changes such as stress pregnancy and immune response (Bike

2003)

Table 2 Some blood parameters (MeanplusmnSE) of ewes in different treatment groups

Treatment Group

WBe Count

pev Lymphocyte 00

Monocyte

Neutrophil

Acidophil 1

Basophil

Control (c)

a SISOplusmnS2

a 323SplusmnO34

3913plusmnO33 a

649plusmnOO7 a

4SS4plusmnO2 a

344plusmnO13 a

27SplusmnO19 a

Vit E (T J)

Vit E +Se (T2)

b 13 200plusmnS2

c 16200plusmn103

b 3637plusmn 124

c 3693plusmn 140

43S3plusmn1 16 b

4S23plusmn14S c

79plusmnO46 b 748plusmnO3S

b

4197plusmn 10 b

4264plusmn 13S b

401plusmnObs A 208plusmnO31 A

266plusmnO32a

lA9plusmnO23 a

Mean with different letters in the same column refer to significant (PltO05) differences

4

rev data in this study showed significant ((PltO05) increase in rev Values Those alues were

3235LO34 363 7l 124 and 3693plusmn 14 in C T I and T2 groups respectively (Table2) Thi Illay

be related to RBC count The elevation of RBC count in blood increased with supplemenLalion

or Vit E and Vit E +Se This elevation ma be related to the antioxidant effect of Vit E and Sc

Ihal r cil Ih h middot nwlysis of RH in hlood (Si bull 1992)

Differential white cell count showed that difterenccs were significant (PltO05 ) between

treatm ~n t groups Lymphocyte percent increased significantly (PltO05) in T group (4353plusmn

1(6) 12 roup 4S23plusmnI 4S) in compari on to C group (3913plusmnO33) In the same way

monocyte percent increased (C 649plusmn007 T 79plusmn046 and T2748plusmn035) On the other

hand percent of Neutrophi l decreased in T and T2 in comparison to C group (TabJe2) These

changes in percents of differential whit cell may refer to positiv improvement of the

immune response in the body The effect of Vit and lh combined role of Vit +Se may

inlrease the viscosity of phagocytic celJ membrane (Bendich 19l)O) In addition to the

possible elevation of immunoglobulin production (Reddy el aI ] 986 and st Laurent ef al

1990)

Results of tht cfrect or supplementati on of Vit E and Vit E+Se on some reproductive traiL are

shown in (Table 3) E lrus wa middot synchronized in all animals in di fferent groups (JOO) Further

more all ewes were mated (100) Ilowcvcr fertility rate was 80100 and IOOdeg(Jin C Tl and 12

group respectively It was clear that fertility was improved with supplementation of Vit E and

Vit + c

This may be due to the protection of gonadotrophic receptors from oxidation a accompanied

with elevation or estrogen secretion (Segerson ef a i 1980)

5

Introduction

Many new techniques have been developed during Lhc last decadls Thcst lechniques ere

approached to obtain the highest improvement of reproductive tniit of animals Arti licial

insemination and hormonal treatmcnts super ovulation and embryo transfcr (Bearden and

Puguay 1917) w~n laruple~ uf these new Lellmiques UtaL have been applied to Improve

rcproductive performance Also new studies have been referred to use one of thc metabolic

signals to stimulate gonadotrophic hormonal release (Hall et al 1992) Speci fie nutrients that are

involved in r production (Martin and Wa lkcd-Brown 1995) growth and development (Lindsay

et al 1993 Fosler 1994) may be used for the same goal

Previous reports have suggested that vitamin E and elcllium (Se) are one of the important

nutrients that can arfect mallY biological processes in the body such as immunity (Hemken et a1

1998 ) Metabolism (Awadeh el ai 1998) and reproduction (Jen) 1996) in addition ViC E and

Se improved spermatogenesis and senien middot quality (Marin-Guzman 1990 Brzezinsksshy

SI -bodzinska ai 199-)

Therefore this study was conducted to evaluate the effect ofVitF and combination of ViLE and

Se on me physiological parameters in Awassi Ewes

Materia ls and Metbods

Thirty Awa si-breed ewes aged between 3 - 35 years with body weight of 50plusmn 127 kg Wlrc

us d in thi s study to determine the effect of injecting Vit E and ornbination of Vit + Selenium

(S) on some blood parameters ego Whi te blood count (WBC) Different WBC Packed Cell

Volume (reV) and I ymphocytes and reproductive performance conception rate feriility and

liLLer ~izc

The e peri mental animals were divided into three equal groups (10) in each group Animals in

group (f 1) wen given Vit - group (T2) were given Vit E + Sc whik group (C) wcre k~pl 1I U

control

Jluc healthy and sound rams aged between (2 5 - 30) yems with all a erage weight or 0 shy

076 kg Vere used for natural mating those experimental ewes at a rate of one ram for (ach ten

ewes ( I 10)

2

All the experimental animals were hou cd in s mi closed bams designed a cording to the

prevai ling type in the area and the tropics Free access to drinking water restricted

conccntrate

(Table I) fed at a rate of hal f kg per head per day and free natural grazing was the pattern or reeding those experimental animals Vito and se were given intramuscularly (or R weeks (4

befon mating and 4 after mating) estrus was synchronized by sponges impregnated with

Progesteronc (containing 40 mg Mcdoxy Progesterone Inter Vet - Holland)

Calculations of the bl od parameters (WBC Deferential WBC and peV) were determined by

llsing Automated Hematological Analyses - known as (SYSMEX)

For the reprodu lion perfomlance synchronized estrus was determined by introducing

vasectomized rams twice a day at early morning and early evening Then thc conception rate

fertili ty litter si7e and lamb birth weight were observed and recorded for analysis

Statistical Analysis

The rccorded data was then collected for stati tical analysis using (Sat Soft Programme 1995)

and (ANOY A) as analytical technique and (L D) was uscd to determine the di rfClcncl between

the means

3

Ration Ingredients age Crude proteiI

Crude Protein 0

in Ration

Barley 60 9 54

Wheat Bran 185 14 middot259

Cotton Seed Cake 20 22 44

Calciul1l I - -Sodium Ch loride 05 - -

100 45 1239

Results and D iscussion

The effect ofVit E (T ) and V it E+Se (T2) on some blood parameters are shown in (Table

2) Results showed that supplementation of Vit E and Se increased significantly (PltO05)

the total number of WBC count in comparison to control group (C) WBC count were

8180 12360 and 16200 in C T I and T2 groups respectively This may be due to the

improvement or immunological function of the body (Meydani 1990) WBe count is an

important tool for diagnos is of the health conditions and problems and may undergo to

many physiological changes such as stress pregnancy and immune response (Bike

2003)

Table 2 Some blood parameters (MeanplusmnSE) of ewes in different treatment groups

Treatment Group

WBe Count

pev Lymphocyte 00

Monocyte

Neutrophil

Acidophil 1

Basophil

Control (c)

a SISOplusmnS2

a 323SplusmnO34

3913plusmnO33 a

649plusmnOO7 a

4SS4plusmnO2 a

344plusmnO13 a

27SplusmnO19 a

Vit E (T J)

Vit E +Se (T2)

b 13 200plusmnS2

c 16200plusmn103

b 3637plusmn 124

c 3693plusmn 140

43S3plusmn1 16 b

4S23plusmn14S c

79plusmnO46 b 748plusmnO3S

b

4197plusmn 10 b

4264plusmn 13S b

401plusmnObs A 208plusmnO31 A

266plusmnO32a

lA9plusmnO23 a

Mean with different letters in the same column refer to significant (PltO05) differences

4

rev data in this study showed significant ((PltO05) increase in rev Values Those alues were

3235LO34 363 7l 124 and 3693plusmn 14 in C T I and T2 groups respectively (Table2) Thi Illay

be related to RBC count The elevation of RBC count in blood increased with supplemenLalion

or Vit E and Vit E +Se This elevation ma be related to the antioxidant effect of Vit E and Sc

Ihal r cil Ih h middot nwlysis of RH in hlood (Si bull 1992)

Differential white cell count showed that difterenccs were significant (PltO05 ) between

treatm ~n t groups Lymphocyte percent increased significantly (PltO05) in T group (4353plusmn

1(6) 12 roup 4S23plusmnI 4S) in compari on to C group (3913plusmnO33) In the same way

monocyte percent increased (C 649plusmn007 T 79plusmn046 and T2748plusmn035) On the other

hand percent of Neutrophi l decreased in T and T2 in comparison to C group (TabJe2) These

changes in percents of differential whit cell may refer to positiv improvement of the

immune response in the body The effect of Vit and lh combined role of Vit +Se may

inlrease the viscosity of phagocytic celJ membrane (Bendich 19l)O) In addition to the

possible elevation of immunoglobulin production (Reddy el aI ] 986 and st Laurent ef al

1990)

Results of tht cfrect or supplementati on of Vit E and Vit E+Se on some reproductive traiL are

shown in (Table 3) E lrus wa middot synchronized in all animals in di fferent groups (JOO) Further

more all ewes were mated (100) Ilowcvcr fertility rate was 80100 and IOOdeg(Jin C Tl and 12

group respectively It was clear that fertility was improved with supplementation of Vit E and

Vit + c

This may be due to the protection of gonadotrophic receptors from oxidation a accompanied

with elevation or estrogen secretion (Segerson ef a i 1980)

5

All the experimental animals were hou cd in s mi closed bams designed a cording to the

prevai ling type in the area and the tropics Free access to drinking water restricted

conccntrate

(Table I) fed at a rate of hal f kg per head per day and free natural grazing was the pattern or reeding those experimental animals Vito and se were given intramuscularly (or R weeks (4

befon mating and 4 after mating) estrus was synchronized by sponges impregnated with

Progesteronc (containing 40 mg Mcdoxy Progesterone Inter Vet - Holland)

Calculations of the bl od parameters (WBC Deferential WBC and peV) were determined by

llsing Automated Hematological Analyses - known as (SYSMEX)

For the reprodu lion perfomlance synchronized estrus was determined by introducing

vasectomized rams twice a day at early morning and early evening Then thc conception rate

fertili ty litter si7e and lamb birth weight were observed and recorded for analysis

Statistical Analysis

The rccorded data was then collected for stati tical analysis using (Sat Soft Programme 1995)

and (ANOY A) as analytical technique and (L D) was uscd to determine the di rfClcncl between

the means

3

Ration Ingredients age Crude proteiI

Crude Protein 0

in Ration

Barley 60 9 54

Wheat Bran 185 14 middot259

Cotton Seed Cake 20 22 44

Calciul1l I - -Sodium Ch loride 05 - -

100 45 1239

Results and D iscussion

The effect ofVit E (T ) and V it E+Se (T2) on some blood parameters are shown in (Table

2) Results showed that supplementation of Vit E and Se increased significantly (PltO05)

the total number of WBC count in comparison to control group (C) WBC count were

8180 12360 and 16200 in C T I and T2 groups respectively This may be due to the

improvement or immunological function of the body (Meydani 1990) WBe count is an

important tool for diagnos is of the health conditions and problems and may undergo to

many physiological changes such as stress pregnancy and immune response (Bike

2003)

Table 2 Some blood parameters (MeanplusmnSE) of ewes in different treatment groups

Treatment Group

WBe Count

pev Lymphocyte 00

Monocyte

Neutrophil

Acidophil 1

Basophil

Control (c)

a SISOplusmnS2

a 323SplusmnO34

3913plusmnO33 a

649plusmnOO7 a

4SS4plusmnO2 a

344plusmnO13 a

27SplusmnO19 a

Vit E (T J)

Vit E +Se (T2)

b 13 200plusmnS2

c 16200plusmn103

b 3637plusmn 124

c 3693plusmn 140

43S3plusmn1 16 b

4S23plusmn14S c

79plusmnO46 b 748plusmnO3S

b

4197plusmn 10 b

4264plusmn 13S b

401plusmnObs A 208plusmnO31 A

266plusmnO32a

lA9plusmnO23 a

Mean with different letters in the same column refer to significant (PltO05) differences

4

rev data in this study showed significant ((PltO05) increase in rev Values Those alues were

3235LO34 363 7l 124 and 3693plusmn 14 in C T I and T2 groups respectively (Table2) Thi Illay

be related to RBC count The elevation of RBC count in blood increased with supplemenLalion

or Vit E and Vit E +Se This elevation ma be related to the antioxidant effect of Vit E and Sc

Ihal r cil Ih h middot nwlysis of RH in hlood (Si bull 1992)

Differential white cell count showed that difterenccs were significant (PltO05 ) between

treatm ~n t groups Lymphocyte percent increased significantly (PltO05) in T group (4353plusmn

1(6) 12 roup 4S23plusmnI 4S) in compari on to C group (3913plusmnO33) In the same way

monocyte percent increased (C 649plusmn007 T 79plusmn046 and T2748plusmn035) On the other

hand percent of Neutrophi l decreased in T and T2 in comparison to C group (TabJe2) These

changes in percents of differential whit cell may refer to positiv improvement of the

immune response in the body The effect of Vit and lh combined role of Vit +Se may

inlrease the viscosity of phagocytic celJ membrane (Bendich 19l)O) In addition to the

possible elevation of immunoglobulin production (Reddy el aI ] 986 and st Laurent ef al

1990)

Results of tht cfrect or supplementati on of Vit E and Vit E+Se on some reproductive traiL are

shown in (Table 3) E lrus wa middot synchronized in all animals in di fferent groups (JOO) Further

more all ewes were mated (100) Ilowcvcr fertility rate was 80100 and IOOdeg(Jin C Tl and 12

group respectively It was clear that fertility was improved with supplementation of Vit E and

Vit + c

This may be due to the protection of gonadotrophic receptors from oxidation a accompanied

with elevation or estrogen secretion (Segerson ef a i 1980)

5

Ration Ingredients age Crude proteiI

Crude Protein 0

in Ration

Barley 60 9 54

Wheat Bran 185 14 middot259

Cotton Seed Cake 20 22 44

Calciul1l I - -Sodium Ch loride 05 - -

100 45 1239

Results and D iscussion

The effect ofVit E (T ) and V it E+Se (T2) on some blood parameters are shown in (Table

2) Results showed that supplementation of Vit E and Se increased significantly (PltO05)

the total number of WBC count in comparison to control group (C) WBC count were

8180 12360 and 16200 in C T I and T2 groups respectively This may be due to the

improvement or immunological function of the body (Meydani 1990) WBe count is an

important tool for diagnos is of the health conditions and problems and may undergo to

many physiological changes such as stress pregnancy and immune response (Bike

2003)

Table 2 Some blood parameters (MeanplusmnSE) of ewes in different treatment groups

Treatment Group

WBe Count

pev Lymphocyte 00

Monocyte

Neutrophil

Acidophil 1

Basophil

Control (c)

a SISOplusmnS2

a 323SplusmnO34

3913plusmnO33 a

649plusmnOO7 a

4SS4plusmnO2 a

344plusmnO13 a

27SplusmnO19 a

Vit E (T J)

Vit E +Se (T2)

b 13 200plusmnS2

c 16200plusmn103

b 3637plusmn 124

c 3693plusmn 140

43S3plusmn1 16 b

4S23plusmn14S c

79plusmnO46 b 748plusmnO3S

b

4197plusmn 10 b

4264plusmn 13S b

401plusmnObs A 208plusmnO31 A

266plusmnO32a

lA9plusmnO23 a

Mean with different letters in the same column refer to significant (PltO05) differences

4

rev data in this study showed significant ((PltO05) increase in rev Values Those alues were

3235LO34 363 7l 124 and 3693plusmn 14 in C T I and T2 groups respectively (Table2) Thi Illay

be related to RBC count The elevation of RBC count in blood increased with supplemenLalion

or Vit E and Vit E +Se This elevation ma be related to the antioxidant effect of Vit E and Sc

Ihal r cil Ih h middot nwlysis of RH in hlood (Si bull 1992)

Differential white cell count showed that difterenccs were significant (PltO05 ) between

treatm ~n t groups Lymphocyte percent increased significantly (PltO05) in T group (4353plusmn

1(6) 12 roup 4S23plusmnI 4S) in compari on to C group (3913plusmnO33) In the same way

monocyte percent increased (C 649plusmn007 T 79plusmn046 and T2748plusmn035) On the other

hand percent of Neutrophi l decreased in T and T2 in comparison to C group (TabJe2) These

changes in percents of differential whit cell may refer to positiv improvement of the

immune response in the body The effect of Vit and lh combined role of Vit +Se may

inlrease the viscosity of phagocytic celJ membrane (Bendich 19l)O) In addition to the

possible elevation of immunoglobulin production (Reddy el aI ] 986 and st Laurent ef al

1990)

Results of tht cfrect or supplementati on of Vit E and Vit E+Se on some reproductive traiL are

shown in (Table 3) E lrus wa middot synchronized in all animals in di fferent groups (JOO) Further

more all ewes were mated (100) Ilowcvcr fertility rate was 80100 and IOOdeg(Jin C Tl and 12

group respectively It was clear that fertility was improved with supplementation of Vit E and

Vit + c

This may be due to the protection of gonadotrophic receptors from oxidation a accompanied

with elevation or estrogen secretion (Segerson ef a i 1980)

5

rev data in this study showed significant ((PltO05) increase in rev Values Those alues were

3235LO34 363 7l 124 and 3693plusmn 14 in C T I and T2 groups respectively (Table2) Thi Illay

be related to RBC count The elevation of RBC count in blood increased with supplemenLalion

or Vit E and Vit E +Se This elevation ma be related to the antioxidant effect of Vit E and Sc

Ihal r cil Ih h middot nwlysis of RH in hlood (Si bull 1992)

Differential white cell count showed that difterenccs were significant (PltO05 ) between

treatm ~n t groups Lymphocyte percent increased significantly (PltO05) in T group (4353plusmn

1(6) 12 roup 4S23plusmnI 4S) in compari on to C group (3913plusmnO33) In the same way

monocyte percent increased (C 649plusmn007 T 79plusmn046 and T2748plusmn035) On the other

hand percent of Neutrophi l decreased in T and T2 in comparison to C group (TabJe2) These

changes in percents of differential whit cell may refer to positiv improvement of the

immune response in the body The effect of Vit and lh combined role of Vit +Se may

inlrease the viscosity of phagocytic celJ membrane (Bendich 19l)O) In addition to the

possible elevation of immunoglobulin production (Reddy el aI ] 986 and st Laurent ef al

1990)

Results of tht cfrect or supplementati on of Vit E and Vit E+Se on some reproductive traiL are

shown in (Table 3) E lrus wa middot synchronized in all animals in di fferent groups (JOO) Further

more all ewes were mated (100) Ilowcvcr fertility rate was 80100 and IOOdeg(Jin C Tl and 12

group respectively It was clear that fertility was improved with supplementation of Vit E and

Vit + c

This may be due to the protection of gonadotrophic receptors from oxidation a accompanied

with elevation or estrogen secretion (Segerson ef a i 1980)

5

Table 3 Some Reproductive t raits-of ewes in different treatment groups

Treatment Group

Number of ewes

00 Estrus

Synch ronization

Number Ewes mated

Number of Ewes

Lambed

00

Fertility

Control (c)

10 100 II to n 8 a 80 n

Vit E (T I )

10 100 a lOa 10 b 100 b

Vit E +Se (T2)

10 100 a to II 10 b 100 b

bull Numbers with different letters refer to significant (Plt005) difference

Lambing data that refer to th number of lamhs lamb weight and lamhing rate are

shown in (Table 4) Numher of lamb were higher (PltO05) in ) (10) and T2 (14)

groups compared to C group (8) Therefore lambing p rcent w re higher (PltO05) in

T ) (100) and T2 (140) in comparison to C group (80) Titter size was 08 10 and 14 in

C I I and T~ groups respectively Differences in lambs weight were not significant

(Pgt005) The improvement of lambing data due to Vit E +Se suppl mentation whkh

may probably causlt follicular development and increae secretion oj estrogen Ievds

that cau C a posi tive feedback on Ll I Secretion (Fuquay ) 997) This study was in

agreement with Malec i eta 2002 who found a higher fertility in sheep treated with

Vit F and Se

Table 4 Lambing data of different treatment groups

Treatment Group

lambed NO of ~ambs weight Lam bs born (mean plusmn SE)

lambing Litter ize

Control I(c)

JO 8a 411plusmn021 a 80a 08

Vit E 10 10 b 464plusmn025 a 100 b 10 (Td Vit E +Se 10 14 c 413plusmn043 a 140 c 14 (T2)

bull Numbers with different letters refer to significant (Plt005) differences

6

1n conclusion the resul t or this stud showed that Supplementation of Vil E and Se could improve omc blood parameters and ome reproductive traits in female sheep

Acknowledgemen ts

I hanks ami gralitudc arc (Iue LO llle staIr or lilt Illini tty uf h~lIlth llllli Ih I middothni iuIl D in

the ministry of animal heal th and animal production in Arab Republic of Syria lor

providing the experimental animal and other materials The authors also extend their

thanks and appreciation to the staff of the Department of Animal Production Collegt of

Agricultural Studies of the Sudan University of Science and Technology for their

unlimited support

References

Awadeb FT Kincaid RL and Johnson KA (1998) Effect of level and source

of dietary selenium on concentration of thyroid hOlmoDeS and

immunoglobulin in beef CoWS aDd calves bullJ AnimSci 76 1204-1215

(41hBearden HJ and Fuguay JW (1997) Applied Animal Reproduction

ed) Prentice- Hall Upper - Saddle River New Gersy USA

Bandich A (1990) Antioxidant Nutrients and Immune Function Introduction

Advances in Expt Medicine and Biology 2621 -12

Bike (2003) Influence of Selenium and Iodine supplementation on thyroid

hormone concentrations in the b lood serum of sheep vol 59(12) 1053shy

1156

FostCI DL (1994) Puberty in the female sbeep Physiology of Reprod (2nd

cd) 241 1-452

Hemken R W Hovman RJ and Trammell S (1998) Selenium for dairy cattle

A role for organic selenium in biotechnology in feed industry Proc

Altech 14th Ann Sym p T P Lyons and KA Jacaues Eds Nottingham

Univ Press

Hall JB Schillo KK Hileman SM and Boiling JA (1992) Dose tyrosine act

as a national signal mediating the effects of increased feed intake on

Luteniziog hormone pa tter ns in growth restricted lambs BioI Reprod

46573-582

7

1n conclusion the resul t or this stud showed that Supplementation of Vil E and Se could improve omc blood parameters and ome reproductive traits in female sheep

Acknowledgemen ts

I hanks ami gralitudc arc (Iue LO llle staIr or lilt Illini tty uf h~lIlth llllli Ih I middothni iuIl D in

the ministry of animal heal th and animal production in Arab Republic of Syria lor

providing the experimental animal and other materials The authors also extend their

thanks and appreciation to the staff of the Department of Animal Production Collegt of

Agricultural Studies of the Sudan University of Science and Technology for their

unlimited support

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46573-582

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8

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