Desired Gene

Restriction Enzymes Bacterial defense against viral DNA Cut DNA at specific sequences

CCTTTG AATTCCCAGAATCGGAAACTTAA GGGTCTTAG AATTCGGCCATATACG GCCGGTATATGCTTAA

Restriction Sitesfor EcoRI

Electrophoresis Separation of molecules based on size Negatively charged DNA molecules are

pulled through a gel by an electrical field Smaller molecules travel faster and farther

DNA FingerprintingVariations in DNA

Variations in restriction sites

Variations in fragment sizes

Variations in electrophoresis bands

Walter

Jordan

Poop

GeneCloning

Binary Fission

Gene Splicing

Transformation

Mammalian Cloning

Somatic cell nuclear transfer

Stem Cells