descriptive chart - proteus vulgaris
DESCRIPTION
Descriptive Chart of Proteus vulgarisTRANSCRIPT
Descriptive Chart
Culture No.: 61
Organism: Proteus vulgaris
Morphological Characteristics
Cell Shape: Bacillus (rod)
Arrangement: swarm
Size: 0.4 to 0.6 micrometers by 1.2 to 2.5 micrometers
Spores: no
Gram’s Stain: negative
Motility: yes
Capsules: no
Cultural Characteristics
Nutrient Agar: Colonies clumped together
Blood Agar: Positive for hemolysis
Agar Slant: positive for growth
Nutrient Broth: positive for growth
Gelatin Slab: positive for motility and liquefaction
Oxygen Requirements: facultative anaerobic
Optimum Temp.: 23° C
Physiological Characteristics
Tests ResultsF
erm
enta
tion
Glucose Yes; yellow
Lactose No; orange/pink
Sucrose Yes; yellow/orange
Mannitol No; orange/pink
Hyd
roly
sis
Gelatin Liquefaction Positive
Starch Negative
Casein Positive
Fat Positive
IMV
iC
Indole Positive (red floating layer)
Methyl Red Yes
V-P (acetylmethylcarbinol) Negative
Citrate Utilization Negative
Nitrate Reduction Positive after Zn added
H2S Production Positive (black)
Urease Positive (light pink)
Catlase Yes
Oxidase Negative
DNase
Phenylalanase ?postive (slight green)