derivation of ils & iss
DESCRIPTION
LXS RI PANEL: LARGEST EXTANT RI SERIES 78 STRAINS BETH BENNETT T.E. JOHNSON UNIV OF COLORADO BOULDER, CO. DERIVATION OF ILS & ISS. 8 WAY CROSS: A, AKR, BALB, C3H, C57, DBA, ISBI, RIII. HETEROGENOUS STOCK (HS). Select for differential ethanol sensitivity for 25 generations. LS & SS. - PowerPoint PPT PresentationTRANSCRIPT
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LXS RI PANEL:LARGEST EXTANT RI SERIES
78 STRAINS
BETH BENNETTT.E. JOHNSON
UNIV OF COLORADOBOULDER, CO
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DERIVATION OF ILS & ISS
8 WAY CROSS: A, AKR, BALB, C3H, C57, DBA, ISBI, RIII
HETEROGENOUS STOCK (HS)
LS & SS
ILS & ISS
Select for differential ethanolsensitivity for 25 generations
Inbreed 20 generations
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RI CONSTRUCTION
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BODY WT IN FEMALES
-Female weight chosen as mapping character-Not selected in progenitor strains-Possible interaction with fertility, lifespan-Easily measured test phenotype for LXS RI
-Measured at 30 day intervals in generation 13-94% inbred, 89% loci fixed
-Heritability estimated-QTLs mapped using MapManagerQT
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GenotypingR.Williams and L. Lu
-Begun at N18
-All Parents within Strain Genotyped-Expect 96% of Loci Fixed-361 Polymorphic Markers Identified-Approximate Marker Density of 4.5 cM
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30 DAY WEIGHTS
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WT DISTRIBUTION AT 60, 90, 120, 150 DAYS
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Heritability (h2), Genetic correlation (rG), and Phenotypic correlation (rP)
30 Day 60 Day 90 Day 120 Day 150 Day
30 Day .49 .64 .53 .57 .56
60 Day .57 .62 .79 .83 .80
90 Day .49 .88 .66 .86 .91
120 Day .53 .83 .90 .64 .95
150 Day .53 .84 .89 .92 .72
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FEMALE BODY WEIGHT IN LXS RIs AT 30 DAY INTERVALS
0
5
10
15
20
25
30
35
9 11 13 15 17 19 21 23 25 27
306090120150
WT IN GRAMS
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GROWTH RATE AT 30 DAY INTERVALS
3.75
3.50
3.25
3.00
2.75
2.50
2.25
2.00
1.75
1.50
1.25
1.00
.75
.50
.25
0.00
-.25
-.50
-.75
-1.00
-1.25
A. 30-60 Days120
100
80
60
40
20
03.75
3.50
3.25
3.00
2.75
2.50
2.25
2.00
1.75
1.50
1.25
1.00
.75
.50
.25
0.00
-.25
-.50
-.75
-1.00
-1.25
B. 60-90 Days200
180
160
140
120
100
80
60
40
20
0
Gms Gained per Week
3.75
3.50
3.25
3.00
2.75
2.50
2.25
2.00
1.75
1.50
1.25
1.00
.75
.50
.25
0.00
-.25
-.50
-.75
-1.00
-1.25
C. 90-120 Days
N o
f M
ice
120
100
80
60
40
20
0
Gms Gained per Week
3.75
3.50
3.25
3.00
2.75
2.50
2.25
2.00
1.75
1.50
1.25
1.00
.75
.50
.25
0.00
-.25
-.50
-.75
-1.00
-1.25
D. 120-150 Days
N o
f M
ice
100
80
60
40
20
0
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h2 for Growth Rate
Interval (Days)
Mean Growth Rate + SE
N strains
h2 (95% CI)
30-60 1.20 + 0.004 97 .48 (.39-.56)
60-90 0.49 + 0.003 86 .42 (.32-.50)
90-120 0.37 + 0.003 75 .25 (.15-.35)
120-150 0.25 + 0.002 63 .22 (.09-.33)
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QTL MAPPING FOR BODY WT AT 30 DAYS
-2-101234567
D2Mit3
59
D2Mit8
1
D2Mit3
67
D2Mit9
0
D2Mit9
4
D2Mit4
03
D2Mit2
55
D2Mit2
57
D2Mit1
45
Chromosome 2
LRSEffect
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QTL MAPPING FOR BODY WT AT 60 DAYS
-202468
1012141618
D11
Mit
2
D11
Mit
151
D11
Mit
173
D11
Mit
318
D11
Mit
90
D11
Mit
179
D11
Mit
360
D11
Mit
333
D11
Mit
11
Chromosome 11
LRSEffect
LOD=3.4
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QTL MAPPING FOR BODY WT AT 90, 120 & 150 DAYS
-202468
10121416
D5M
it34
6
D5M
it34
4
D5M
it29
4
D5M
it25
1
D5M
it38
7
D5M
it26
7
D5M
it55
D5M
it30
9
D5M
it40
3
D5M
it27
8
D5M
it13
9
D5M
it37
2
D5M
it37
2
Chromosome 5
LRS90Effect90LRS120Effect120LRS150Effect150
LOD=3.2
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PHENOTYPES BEING ASSESSED IN LXS RI
-ETHANOL STUDY:-Ethanol sensitivity & blood ethanol at awakening-Ethanol tolerance-Low dose ethanol activationAGING STUDY:-Lifespan-Dietary restriction responses
-Torpor, Food processing efficiency, Hair growth rate