Upload File

depression)and)per2%heldgen564s14.weebly.com/uploads/2/6/0/1/26017349/per2_final... ·...

  • Home
  • Documents
  • Depression)and)PER2%heldgen564s14.weebly.com/uploads/2/6/0/1/26017349/per2_final... · Whatis)depression?))))) Low)mood)and)sadness)that interferes)with)daily)life))) Affects)6.7%)of)the)adult
18
Depression and PER2 Andie Held http://www.genengnews.com/gen-news-highlights/genetic-link-for-depression-furthered-by-analysis-of-54-studies/81244463/

Upload: others

Post on 07-Oct-2020

2 views

Category:

Documents


0 download

Report

TRANSCRIPT

Page 1: Depression)and)PER2%heldgen564s14.weebly.com/uploads/2/6/0/1/26017349/per2_final... · Whatis)depression?))))) Low)mood)and)sadness)that interferes)with)daily)life))) Affects)6.7%)of)the)adult

Depression  and  PER2  Andie  Held  

http://www.genengnews.com/gen-news-highlights/genetic-link-for-depression-furthered-by-analysis-of-54-studies/81244463/

Page 2: Depression)and)PER2%heldgen564s14.weebly.com/uploads/2/6/0/1/26017349/per2_final... · Whatis)depression?))))) Low)mood)and)sadness)that interferes)with)daily)life))) Affects)6.7%)of)the)adult

What  gets  you  down  in  the  dumps?  

h8p://www.desibucket.com/db/02/25158/25158.jpg  

Page 3: Depression)and)PER2%heldgen564s14.weebly.com/uploads/2/6/0/1/26017349/per2_final... · Whatis)depression?))))) Low)mood)and)sadness)that interferes)with)daily)life))) Affects)6.7%)of)the)adult

What  is  depression?          

Low  mood  and  sadness  that  interferes  with  daily  life      Affects  6.7%  of  the  adult  populaKon  in  the  U.S.      A8ributable  to  geneKc,  biological,  environmental,  and  psychological  factors                  

http://www.steveseay.com/depression-symptoms-major-depressive-disorder/

Page 4: Depression)and)PER2%heldgen564s14.weebly.com/uploads/2/6/0/1/26017349/per2_final... · Whatis)depression?))))) Low)mood)and)sadness)that interferes)with)daily)life))) Affects)6.7%)of)the)adult

PER2,  Who?  

Light  recepKon  RegulaKon  of  circadian  

rhythm  

Always  found  C-­‐terminal  to  PAS  

h8p://www.huffingtonpost.com/dr-­‐susan-­‐taylor/can-­‐race-­‐possibly-­‐influen_b_1914649.html;  h8p://2.bp.blogspot.com/-­‐Ph9jPyaTtSs/UGiFJIAiECI/AAAAAAAAAlc/7FGx6NV4LSc/s1600/Day_And_Night_for_draw.jpeg;  h8p://assets.openstudy.com/updates/a8achments/513c2f1ae4b01c4790d24e4d-­‐easyuser-­‐1362926137205-­‐dopamine_chemical_structure.png    

Protein  from  Pfam  

L    

Page 5: Depression)and)PER2%heldgen564s14.weebly.com/uploads/2/6/0/1/26017349/per2_final... · Whatis)depression?))))) Low)mood)and)sadness)that interferes)with)daily)life))) Affects)6.7%)of)the)adult

Human    

Chimp    

Rhesus  Monkey  

Dog  

Ca8le  

Mouse  

Rat  

Gorilla  

Zebrafish  

1255  

How  well  conserved  is  PER2?  

1310  

1266  

1265  

1291  

1257  

1257  

1344  

1404  

%  IdenKty  

91%  

93%  

73%  

73%  

77%    

77%    

99%  

51%  

Page 6: Depression)and)PER2%heldgen564s14.weebly.com/uploads/2/6/0/1/26017349/per2_final... · Whatis)depression?))))) Low)mood)and)sadness)that interferes)with)daily)life))) Affects)6.7%)of)the)adult

Phylogram  from  Clustal  Omega  

How  well  conserved  is  PER2?  

Page 7: Depression)and)PER2%heldgen564s14.weebly.com/uploads/2/6/0/1/26017349/per2_final... · Whatis)depression?))))) Low)mood)and)sadness)that interferes)with)daily)life))) Affects)6.7%)of)the)adult

Are  there  natural  remedies  for  depression?  

 h8p://www.herbalextractsplus.com/images/herbs/valerian-­‐isp.jpg    

Page 8: Depression)and)PER2%heldgen564s14.weebly.com/uploads/2/6/0/1/26017349/per2_final... · Whatis)depression?))))) Low)mood)and)sadness)that interferes)with)daily)life))) Affects)6.7%)of)the)adult

Primary  goal  

 The  primary  goal  of  this  study  is  to  determine  how  the  light/dark  cycle  affects  dopamine  receptor  expression  as  well  as  how  natural  

products  such  as  valerian  affect  gene  expression  

Page 9: Depression)and)PER2%heldgen564s14.weebly.com/uploads/2/6/0/1/26017349/per2_final... · Whatis)depression?))))) Low)mood)and)sadness)that interferes)with)daily)life))) Affects)6.7%)of)the)adult

Which  model  organisms  should  we  use  to  study  depression?    

h8p://1.bp.blogspot.com/_FY8xQMCUsY8/S_MFEQzaSNI/AAAAAAAAACo/aC1euzhdlfU/s1600/group%2520diverse%2520happy%2520people%2520black%2520white%2520brown%2520smile%2520old%2520young%2520teen.jpg;  h8p://www.renalgenes.org/zebrafish.html;  h8p://www.ryanphotographic.com/characidae.htm    

Similarity  percents  determined  by  BLAST  

Page 10: Depression)and)PER2%heldgen564s14.weebly.com/uploads/2/6/0/1/26017349/per2_final... · Whatis)depression?))))) Low)mood)and)sadness)that interferes)with)daily)life))) Affects)6.7%)of)the)adult

Aim  1:  How  well  conserved  is  PER2  in  blind  cavefish?    

Alignments  courtesy  of  Clustal  Omega    

Page 11: Depression)and)PER2%heldgen564s14.weebly.com/uploads/2/6/0/1/26017349/per2_final... · Whatis)depression?))))) Low)mood)and)sadness)that interferes)with)daily)life))) Affects)6.7%)of)the)adult

Aim  2:  How  are  PER2  and  D2  dopamine  receptors  expressed  in  light/dark  environments  in  species  with  different  eye  structures?  

Page 12: Depression)and)PER2%heldgen564s14.weebly.com/uploads/2/6/0/1/26017349/per2_final... · Whatis)depression?))))) Low)mood)and)sadness)that interferes)with)daily)life))) Affects)6.7%)of)the)adult

RNA-­‐seq   RNA-­‐seq  

Page 13: Depression)and)PER2%heldgen564s14.weebly.com/uploads/2/6/0/1/26017349/per2_final... · Whatis)depression?))))) Low)mood)and)sadness)that interferes)with)daily)life))) Affects)6.7%)of)the)adult

Aim  3:  How  does  valerian  root  affect  PER2  protein  expression?  

Page 14: Depression)and)PER2%heldgen564s14.weebly.com/uploads/2/6/0/1/26017349/per2_final... · Whatis)depression?))))) Low)mood)and)sadness)that interferes)with)daily)life))) Affects)6.7%)of)the)adult

Label  free  quan,fica,on;  

Mass  spectrometry    

Label  free  quan,fica,on;  

Mass  spectrometry    

Page 15: Depression)and)PER2%heldgen564s14.weebly.com/uploads/2/6/0/1/26017349/per2_final... · Whatis)depression?))))) Low)mood)and)sadness)that interferes)with)daily)life))) Affects)6.7%)of)the)adult

Homologs  and  flowchart  picture  references  

•  Proteins  on  slide  6  are  curtsey  of  Homologene  •  Sun  h8p://www.clipartbest.com/cliparts/9cz/EMK/9czEMKnKi.gif  •  Moon  h8p://bestclipartblog.com/clipart-­‐pics/moon-­‐clip-­‐art-­‐11.gif  •  Fish  

h8p://www.clker.com/cliparts/r/C/E/Y/M/x/simple-­‐fish-­‐md.png  •  Fish  Food  

h8p://i.walmarKmages.com/i/p/00/04/67/98/77/0004679877035_500X500.jpg    

•  Valerian  root  h8p://www.herbalextractsplus.com/images/herbs/valerian-­‐isp.jpg    

•  Brain  h8p://staKc.freepik.com/free-­‐photo/cartoon-­‐brain-­‐outline-­‐clip-­‐art_411969.jpg    

Page 16: Depression)and)PER2%heldgen564s14.weebly.com/uploads/2/6/0/1/26017349/per2_final... · Whatis)depression?))))) Low)mood)and)sadness)that interferes)with)daily)life))) Affects)6.7%)of)the)adult

Future  DirecKons  GeneKcs:  •  Further  invesKgate  the  role  of  dopamine  in  depression  and  other  natural  products  that  regulate  it  

•  Duplicate  specific  aim  experiments  with  the  addiKon  of  depression  trigger  events    

 Social  Work:  •  Educate  the  public  to  help  fight  the  mental  illness  sKgma  

•  Advocate  for  equal  access  to  mental  health  care    

Page 17: Depression)and)PER2%heldgen564s14.weebly.com/uploads/2/6/0/1/26017349/per2_final... · Whatis)depression?))))) Low)mood)and)sadness)that interferes)with)daily)life))) Affects)6.7%)of)the)adult

Resources  

h8p://www.dbsalliance.org/site/PageServer?pagename=home;  h8p://www.nami.org/Content/NavigaKonMenu/Campaign_for_the_Mind_of_America/Mental_Illness_Awareness_Week/MIAW-­‐sKcker-­‐circle-­‐2.5.jpg;  h8p://www.suicideprevenKonlifeline.org/    

Page 18: Depression)and)PER2%heldgen564s14.weebly.com/uploads/2/6/0/1/26017349/per2_final... · Whatis)depression?))))) Low)mood)and)sadness)that interferes)with)daily)life))) Affects)6.7%)of)the)adult

QuesKons?  

h8p://chibird.com/post/74679635068/a-­‐li8le-­‐posiKve-­‐bunny-­‐post-­‐to-­‐brighten-­‐up-­‐your    


CLOCK, PER2 and BMAL1 DNA methylation: Association with ...dadun.unav.edu/bitstream/10171/28108/1/Chronobiol Int revised.pdf1 CLOCK, PER2 and BMAL1 DNA methylation: Association with

Carbon source affects PKA-dependent polarity of Neurospora ...yardeno/OdedsPDFs/Ziv-etal-08.pdf · Carbon source affects PKA-dependent polarity of Neurospora crassa in a CRE-1-dependent

Depression And Mood Disorders During And After Pregnancy · Depression, Prenatal Depression, Postnatal Depression, or Perinatal Depression. Depression and mood disorders related to

DifferentRolesofNegativeandPositiveComponentsofthe ... · wild-type, Per2 m/m / Per2, / **, / *,

Circadian expression of the clock gene Per2 is altered in ...doc.rero.ch/record/4325/files/1_albrecht_cec.pdf · 1 Circadian expression of the clock gene Per2 is altered in the ruin

Executive Market Segmentation: How Local Density Affects

Mutation of a PER2 phosphodegron perturbs the circadian ... · 4/29/2020  · The PER2-S478A Mutation Lengthens the Period of Behavioral Rhythms. The phosphoswitch model holds that

2011 - Linosnede 2e klas per2

Alg1 per2 5 18 transformations

How the Design of Presentation Slides Affects Audience ......How the Design of Presentation Slides Affects Audience Comprehension: A Case for the Assertion–Evidence Approach* JOANNA

Hoover and the Great Depression. AP Outline Depression, 1929-1933 Depression, 1929-1933 Wall Street crash Wall Street crash Depression economy Depression

Covalent Inhibition of Ubc13 Affects Ubiquitin Signaling ...gloverlab.biochem.ualberta.ca/secondarypages/publications/pdf/glov… · Covalent Inhibition of Ubc13 Affects Ubiquitin

Bad Boys: How Criminal Identity Salience Affects Rule Violation · Bad Boys: How Criminal Identity Salience Affects Rule Violation ... Giuseppe Ugazio and Sophia Zügel for providing

booker.davis704383 per2 paper · 2019. 8. 15. · booker.davis704383_per2_paper Created Date: 1/22/2015 9:13:49 AM

Per2 logika umn

Text–background polarity affects performance irrespective ... · Text–background polarity affects performance irrespective of ambient illumination ... text is particularly poor

Internal Parasites of Livestock Jennifer Edmiston Per2 4/07/02

Casein Kinase 1 dynamics underlie the PER2 circadian ... · stabilizing PER2 to significantly compromise circadian rhythms (Reischl et al., 2007) in a manner similar to its constitutive

CK1δ/ε protein kinase primes the PER2 circadian …CK1δ/e protein kinase primes the PER2 circadian phosphoswitch Rajesh Narasimamurthya, Sabrina R. Huntb, Yining Luc,d, Jean-Michel

Circadian Control of Cell Cycle Progression...transcription factor in the presence of Per2, showing that the Per2-p53 complex prevents p53 from binding to DNA. This implies that the

chapter 4. type 25 · tool, then click the Format-ting Affects Text button. You can also click the Formatting Affects Text button in the Swatches palette. ... inset, as specified

How education affects women’s allocation of time: evidence

Circadian PER2::LUC rhythms in the olfactory bulb of ... · a clock gene product PER2 in the SCN and OB of freely moving mice by means of a bioluminescence reporter and an optical

Gut check: GI tract affects overall health

Colloidal Aggregation Affects the Efficacy of Anticancer ... · Colloidal Aggregation Affects the Efficacy of Anticancer Drugs in Cell Culture Shawn C. Owen,†,§ Allison K. Doak,‡,§

Ie242 ch5-supplement-per2

How$Real)World$Trading$Affects$ Video$Games$ · How$Real)World$Trading$Affects$ Video$Games$ RuneScape$)$A$Case$Study$ By:$Kenan$Courtney$

Supplementary Information Figures 1-19 Adora2b-elicited Per2 stabilization promotes

DEPRESSION. Cognitive explanations of depression

How Exposure to Violence Affects Ethnic Voting

Depression and PER2

Differential effects of PER2 phosphorylation: molecular ...homepages.gac.edu/~jwotton2/PSY385/EEper2... · Differential effects of PER2 phosphorylation: molecular basis for the human

Viewing Nature Scenes Positively Affects Recovery of

Religion and Depression in Adolescenceftp.iza.org/dp9652.pdf · erature on how density of churches affects attendance. We build instead on ... Depression is measured on the Center

Cognition, Affects et Interaction - Accueil · Cognition,AffectsetInteractionGérard Bailly & Sylvie Pesty 2015cel-01110281(pdf) ActesduTER"Cognition,Affects&Interaction" iii ActesduTER"Cognition,Affects&Interaction"