dengue duo rapid test
TRANSCRIPT
Rapid Dengue Duo Test
(IgG+IgM)A Rapid qualitative Immunochromatographic test
for the simultaneous detection of IgG and IgM Antibodies to Dengue Virus in Human Serum,
Whole Blood or Plasma
DENGUE VIRUSMost common arthropod-borne disease worldwide with an
increasing incidence in the tropical regions
Can be life-threatening
Caused by 4 closely related serotypes (serotypes 1-4)
Genus Flavivirus, Family Flaviviridae
Transmitted by Aedes aegypti and Aedes albopictus mosquitoes
“Breakbone" or “Dandy fever."
DENGUE VIRUS
Infection confers life long immunity
But cross protection between serotypes is of short duration.
Reinfection with different serotype after primary attack is more dangerous causes Dengue
Hemorrhagic Fever.
Aedes albopictusAedes aegypti
Urban dengue “Tiger mosquito” Breeds in clear water stored indoor and
outdoor containers such as old tires, vases, jars and bottles
Rural dengue Breeds in clear water stored indoor and
outdoor containers such as bamboo stumps, empty coconut shells, and tree holes.
SIGNS AND SYMPTOMS Malaise Severe headache High fever and chills Severe back ache, joint
pains, muscular pain
SIGNS AND SYMPTOMS
Temperature may persist for 3 -5 days.
On some occasions once again raises in about 5 – 8 days ( Saddle back fever )
Nausea, vomiting and diarrhea
Red eyes, pain in the eyes
SIGNS AND SYMPTOMS
Biphasic pattern- fever lasts about two to seven days, with a smaller peak of fever at the trailing end of the disease.
Clinically, the platelet count will drop until the patient's temperature is normal.
DENGUE HEMORRHAGIC FEVER
Severe, potentially
deadly infection spread by
certain species of mosquitoes
(Aedes aegypti).
Common in children.
In other ( Adults ) the presence of antibodies
due to previous infection with
different serotype
Initially presents like
classical Dengue infection
DENGUE HEMORRHAGIC FEVER
Symptoms
similar to those of dengue fever, but after several days the patient becomes irritable, restless, and sweaty. These
symptoms are followed by a shock -like state.
PATHOGENESIS
Presence of existing Dengue antibody, associated with fresh viral infection with new serotype complexes and forms within few days of the second dengue infection.
Non neutralizing enhancing antibodies promote infection of higher number of Mononuclear cells.
DIAGNOSISPrimary infection- patients diagnosed with dengue infection in non- endemic areas.
Secondary infection- patients diagnosed with dengue infection in endemic areas.
Hemorrhagic tendency
Thrombocytopenia
Evidence of plasma leakage
Encephalitic occurrences
TREATMENTNo Anti viral therapy available
No Vaccine available
Symptomatic management in majority of cases
Dengue Hemorrhagic fever to be treated with suitable fluid replacement
Increased oral fluid intake is recommended to prevent dehydration.
Supplementation with intravenous fluid may be necessary.
platelet transfusion
RAPID DENGUE DUO TEST
PRINCIPLESUBSTANC
E DETECTED
SAMPLE PORT TEST AREA CONTROL
AREA
Human IgG and IgM
antibodies against Dengue
Virus
Colored- Dengue specific
recombinant antigen-
colloidal gold
conjugate
Specific Human IgG
and IgM binding proteins
Anti-rabbit Ig
antiserum
RAPID DENGUE DUO TEST
Reagents and Materials Supplied
1. FirstSign™- Dengue Duo(WB) Test Card: 25 Individually pouched Card tests with a dessicant pouch and a Sample loop2. Running Reagent – 1 bottle3. Product Insert
RAPID DENGUE DUO TEST
Storage and Stability
The sealed pouches in the test kit and the kit
components
4-30˚C
Testing Procedure
Once the pouch is opened and the card is retrieved, it must be used immediately.
Kit Component
s
Room temperatur
e
Label the card with patient’s
identity
RAPID DENGUE DUO TEST
RAPID DENGUE DUO TEST
5µl of serum/plasma or
whole blood
micropipette
Sample port A
5µl sample loop
kit
Dip the loop into the sample
Blot into the sample
port “A”
Important!
loop does not retrieve
clots or debris from the sample
RAPID DENGUE DUO TEST
4 drops of the Running
Reagent
Reagent port “B”
Read the results after 15 minutes
INTERPRETATION OF RESULTS
Negative result:• The presence of only the single
red/purple colored band in the control window “C” indicates the absence of specific antibodies against Dengue virus or the amount of antibodies is below the detection limit of the test.
INTERPRETATION OF RESULTS
Positive result: IgG and IgM• Appearance of two red/purple colored
bands in the test window at region ‘M’ and region ‘G’
• Presence of Dengue virus specific IgM and IgG antibodies
• Acute secondary infection
Positive result: IgM• Appearance of red/purple colored band
in the test window at region ‘M’• Presence of Dengue virus specific IgM
antibodies• Acute primary infection
INTERPRETATION OF RESULTS
Positive Result: IgG• Appearance of red/purple colored band in
the test window at region ‘G’• Presence of Dengue virus specific IgG
antibodies• Acute secondary infection
INTERPRETATION OF RESULTS
Invalid result• If after 15 minutes no band is visible
either in the test or control window• The test should be repeated with a
new test card
INTERPRETATION OF RESULTS
LIMITATION
Limited to the detection of antibodies against Dengue viruses in serum/ plasma.
Test should be repeated with fresh collected samples if negative results were achieved while clinical symptoms are indicative of Dengue virus.
LIMITATION
A definitive clinical diagnosis should not be based on the results of a single test but should rather be made by a clinician after all clinical findings have been evaluated.
Repeatable reactive specimens should be conformed by another qualified test.