Delfan®

Aton Range

Intro Benefits Aminogram Products Trials

Amino acids: essential elements

• Amino acids are essential for the nutrition of any living organism as they are structural protein units.

• They determine the majority of the most important properties that characterize proteins and control many physiological processes: enzymatic, store, constitution, make-up of the chlorophyll, etc.

Amino acids: essential elements

AMINO ACID: Elementary components of proteins assimilated by plants in a direct manner. They are organic molecules, containing two functional radicals: NH2 - Amino group (alkaline)COOH - Carboxyl Acid group

There are about 20 amino acids in Nature.

PEPTIDES: Two (di-peptide) or several (polypeptide) amino acids joint together.

PROTEINS: formed by established sequences of amino acids.

L-α-amino acids

L-α-amino acid

The presence of an asymmetric carbon confers a particularity on the amino acids, in consequence the amino (-NH2) and acid (-COOH) groups can be located in two positions in space. These two forms are called isomers: Levo (L) or Dextro (D).

D-α-amino acid

L-α-amino acids:

The only amino acids biologically active in plants for protein formation

α carbon

Benefits of the Tradecorp’s Amino Acids Range

1. Anti-stress solution

2. Bio-stimulant effect

3. Energy safe / Fast action

4. Carrier effect (nutrients and pesticides)

5. Nutrition supply

6. Easy to use

7. Improves quality

Benefits: 1. Anti-stress Solution

Decisive solution in critical moments

Applications are especially recommended under stress conditions

Strengths the crops in adverse conditions.

What is plant stress?

Plant stress is an “ALTERATION” in any environmental factor producing an impact

on the plant

This influences the biochemical and physiological response to such alteration, and may lead to

DAMAGES in the plant that changes its development

Plant Stress causes

Climatic Stress:

•Water•Light•Salt/pH•High/Low Temperature•Heavy metal/Nutrients•Pollutant

Stress linked to crop cycle:

•Transplantation•Sprouting•Flowering•Fruit setting•Maturity

Other Stress:

•Plant damages•Phytotoxicity after pesticides application•Damages caused by fungi, nematodes, insects…

Stress responses of plants

Growth•Germination inhibition•Growth reduction•Premature senescence•Reduction in productivity

Physiology•Reduction in water uptake•Altered transpiration rate•Reduction in photosynthesis•Altered respiration•Decrease in N assimilation•Increase content of toxic NH4

Molecular Biology•Decreased protein synthesis•Reduced activity of enzymes•Disorganization of membrane systems •Altered gene expression

Stress responses of plants

Stress↓Growth & protein synthesi

s ↑Ammonia content = Toxicity

Synthesis of aa & proteins

= Detoxification

Tradecorp’s Amino Acids Range

Stress Tolerance Mechanisms

Stress Tolerance Mechanisms:Tradecorp’s Amino Acids

Range acts over:

Synthesis of anti-stress proteins XXXSynthesis of enzymes XXFacilitated membrane transport XXAdjustment to improve hormonal balance XXAccumulation of polyamines (molecules that participates in the plant response to environmental stress)

XX

Strengths cell walls XXChlorophyll activation XXAltered gene expression -

Benefits: 2. Bio-stimulant

Maximum speed in direct absorption and assimilation by the crops, (soil & foliar application)

Efficient and immediate crop recovery after critical periods, due to the high content of protein Nitrogen.

Natural Bio-stimulant of vegetation (leaves, roots, fruits, flowers…) for all crops

Hormone-like effect (boost cellular division, rooting, pollen germination…)

100% free L-α-amino acids, this isomer is the active substance that allows the plant to recover from critical situations.

Mechanism of action

The rapid and efficient supply of L-α-amino acids from Tradecorp’s Amino Acids Range in critical periods reduces the time that the plant suffers stress, minimizing any negative stressful effects:

When to apply

Apply Tradecorp’s Amino Acids Range during stress moments or critical periods of

plant development “CURATIVE”

But “PREVENTION” is always much better

Benefits: 3. Energy Save / Fast action

Energy saving…The processes that plants use to synthesize amino acids (amination and transamination) are particularly expensive in terms of ENERGY. The application of the Tradecorp’s Amino Acids Range allows plants to save this extra energy expenditure since they don’t have to synthesize amino acids and can invest it in other processes.

…and less recovery time: this reduces the recovery time for crops in adverse situations.

Greater speed of uptake: free amino acids are characterized by their low molecular weight as opposed to longer chains of peptides and proteins, more difficult to absorb.

Synthesis of proteins in plants

NO2-

NH4+

Aminoacids

NO3-

Peptides

Proteins

Tradecorp’s Amino Acids Range

Benefits: 4. Carrier effect

Why use Free Amino Acids as a Carrier? 1. Small and neutral molecule2. Penetrate easily3. Don’t require energy to penetrate4. Not linked to chlorophyll activity5. Complexing agent of micronutrients

L-α-amino acids from Tradecorp’s Amino Acids Range have a

complexing capacity with micronutrients (Fe, Zn, Mn, Ca, B & Mo),

improving penetration and assimilation of micronutrients, agrochemical products and fertilizers.

RUBIGAN – Active matter: Fenarimol (Pyrimidine fungicide)

Carrier of Fungicides

Product Dose (ml/100l) % Surface colonized by fungi

Fenarimol 50 37.5

Fenarimol + AA 50+300 6.5

Fenarimol 35 42.5

Fenarimol + AA 35+300 15

Source: Cortellini and Maini (1994) Informatore Fitopatológico Nº 4Source: Cortellini and Maini (1994) Informatore Fitopatológico Nº 4

Benefits: 5. Nutrition supply

• Better use of nitrogen and better microelements uptake (Fe, Zn, Mn, Ca, B & Mo) by the leaves

• Improves uptake of nutrients by the roots

• Organic Carbon and Nitrogen source

• Boost enzymes activity, helping the incorporation of nitrogen into proteins

Benefits: 6. Easy to use

Great Versatility, Maximum Security !• Specially recommended for foliar application• Can be also applied into soil• Good compatibility with most of fertilizers and

agrochemicals• Improves efficacy of agrochemicals thanks to its

acid pH• Better adherence and persistence• Safety application: raw material without chlorine• Save energy and time

Benefits: 7. Improves quality

• Increases the yield (growth and development)

• Increases the quality (size, uniformity, sugar content and color of fruits)

• Stimulates physiological processes (shooting, flowering, pollination and ripening)

• More chlorophyll activity

• Enhances early maturity

• Better protection against stress and resistance vs. diseases

Energy and vigour for your crops !

Complete and balanced Aminogram

• Aminogram is the range of amino acids into a product

• Tradecorp’s Amino Acids Range contains a complete and balanced Aminogram (composition and amount of amino acids).

• The Aminogram is characterized by its optimum ratio among amino acids such as proline, glutamic acid, glycine, alanine and arginine, with the object of maximizing efficiency at the agricultural level.

Glycine26,1%

Glutamic acid10,8%

Alanine10,3%Proline

10,3%

Aspartic acid7,2%

Serine7,1%

Histidine0,4%

Methionine0,9%

Leucine4,9%

Hydroxyproline3,5%

Phenylalanine3,3%

Tyrosine3,2%

Arginine2,8%

Valine2,4%

Lysine2,5%

Isoleucine2,1%

Threonine2,2%

Aminogram

Role of main Amino Acids

PROLINE (role in Abiotic Stress) :

positive effect in water and heat stress,

positive effect in stress caused by soil salinity

reduces water loss from cells & regulates stomata opening

ARGININE (growth Regulator) :

promotes root development

regulates the pectin's formation in the cell wall, improving tissues firmness.

hormone precursor, promotes Ethylene, that delay the leaves senescence

promotes different polyamines (molecules that participates in the plant response to environmental stress: salinity stress, water deficits, anaerobiosis and acid stress).

ASPARTIC ACID and PHENYLALANINE :

Stimulates rooting and improves number of roots

Role of main Amino Acids

METHIONINE (hormone precursor):

promotes Cytokinines and Ethylene,

participates in Auxine transport

boosts root development and cell division.

ALANINE and GLYCINE :

participates in the synthesis of Chlorophyll

reinforces virus resistance

GLUTAMIC ACID (amino acid precursor) :

participates in the synthesis of several amino acids (serine, lysine, proline, valine, alanine, aspartic acid),

promotes different polyamines with high anti-stress effect

The two optical isomers of alanine

Delfan®

CompositionDelfan & Delfan Plus

DELFAN:

Free Amino Acids: 11% w/v (10% w/w)

Total nitrogen (N): 4,7% w/v (4,3% w/w)

Proteic nitrogen (N): 3,3% w/v (3% w/w)

Organic matter: 22% w/v (20% w/w)

DELFAN PLUS:

Free Amino Acids: 28,8% w/v (24% w/w)

Total nitrogen (N): 10,8% w/v (9% w/w)

Proteic nitrogen (N): 6% w/v (5% w/w)

Organic matter: 44,4% w/v (37% w/w)

Aton Range

CompositionAton Range

ATON Ca: Free Amino Acids: 6% w/v (5% w/w)Calcium (CaO): 7,5% w/v (6,3% w/w) ATON Fe: Free Amino Acids: 6% w/v (5% w/w) Iron (Fe): 6,3% w/v (5,3% w/w) ATON Mn: Free Amino Acids: 5,5% w/v (5% w/w)Manganese (Mn): 3,7% w/v (3,4% w/w)ATON Mo: Free Amino Acids: 6,5% w/v (5% w/w)Molybdenum (Mo): 7,85 w/v (6% w/w)Phosphorous (P2O5): 14,8 w/v (11,4% w/w)ATON Zn: Free Amino Acids: 5,5% w/v (5% w/w)Zinc (Zn): 4,9% w/v (4,5% w/w)

CompositionAton Range

ATON AZ:

Free Amino Acids: 6% w/v (5% w/w)

Iron (Fe): 1,1 w/v (0,9% w/w)

Manganese (Mn): 0,8 w/v (0,7% w/w)

Calcium (CaO): 1,2 w/v (1% w/w)

Zinc (Zn): 1,3 w/v (1,1% w/w)

Boron (B): 0,1 w/v (0,09% w/w)

Molybdenum (Mo): 0,1 w/v (0,09% w/w)

Trials

Crop: Melon Durango Hybrid

Product: Delfan®

Crop conditions: Greenhouse, 2 types of soil (sandy and clayey)

Contractor: University Autónoma Ag. Antonio Narro, Mexico

Parameters assessed: Root weight, Total yield and amount of fruits

P erformance of melon planted in two different soil types (University Autónoma Agraria Antonio Narro - Mexico)

0

0,5

1

1,5

2

2,5

3

Clay soil Sandysoil

Clay soil Sandysoil

Clay soil Sandysoil

Root weight (g) Yield (kg) Number of fruits

Control

Delfan

The results of the study indicate that the response of the crop depends on the type of soil and the stage in the crop cycle. However, when Delfan was applied, a trend was observed towards increasing all the parameters in both types of soil, which proves that the application of Delfan promotes the physiological activity, and favours the vigour, resistance and general development of the crop. Delfan performs better with significant differences between both types of soil, between Delfan and the control with regard to the root weight, yield and amount of fruits.

Crop: Potato

Product: Delfan®

Dosage: 1,5 L/ha at the end of bud stage, foliar application

Contractor: Lithuanian University of Agriculture, Lithuania

Parameters assessed: Yield

Potatoes Yield T/ha

+ 4,2 %

30

31

32

33

34

35

36

37

38

Control Delfan

Delfan improves significantly the yield of potatoes in dry year. Delfan is easy to use because it could be mixed with other fertilizers and with plant protection products.

Crop: Carnation variety Cinderela

Product: Delfan® Plus

Dosage: 0,75 cm3/L Foliar application every two weeks

Contractor: Ecoterra, Colombia

Parameters assessed: Production and Quality of cuttings

Total amount of bunches (25 cuttings) harvested per quality grade and treatment

0

10

20

30

40

50

60

70

Control Delfan

Green

Red

Yellow

Blue

Total

Delfan Plus increases in 7,8% the cutting production of mother plants compared to standard treatment. Delfan Plus enhances better quality of cuttings (green and red quality classes are the best to get a successful rooting)

Crop: Tomato (var. Rio Grande)

Product: Delfan® Plus

Dosage: 200-250-300 ml/100 L of water. Foliar application,

1st applic after transplanting and then after bud

formation every two weeks.

Contractor: University Autonoma of Chapingo, Mexico

Parameters assessed: Yield, number of fruits, fresh plant weight

94

124

140

36

0

20

40

60

80

100

120

140

Peso Fresco de Frutos

Delfan Plus 200 ml

Delfan Plus 250 ml

Delfan Plus 300 ml

Testigo

2.66

3.50

4.33

2

0

0.5

1

1.5

2

2.5

3

3.5

4

4.5

Número de Frutos

Delfan Plus 200 ml

Delfan Plus 250 ml

DelFAN Plus 250 ml

Testigo

Yield Number of fruits

Control Control

300 ml

Delfan Plus increases in 60% the total number of fruits and yield. The dosage of 300 ml / 100 L of water is the most effective to improve tomato yield parameters.In this trial was also established that Delfan Plus have no phytotoxic effect on leaves.

Effect of 3 different dosages of Delfan Plus on Tomato Yield parameters

Crop: Avocado nursery

Product: Delfan® + Humistar®

Dosage: 2L Delfan/1000 L. foliar application

2,5L Humistar/1000 L. soak application

Contractor: Fertitec, Peru

Parameters assessed: Plantlet and root development

25

30

35

40

45

50

55

17-march 06-april 12-april 05-may 28-may

cm

Standard

Delfan + Humistar

Delfan®+ Humistar® : bigger plantlets

Plant length evolution

The combination of Delfan and Humistar provides a faster plant development in terms of plant height and root system. Split application of Delfan (foliar application) and soaking of Humistar have synergic effects to boost the quality and health of the plantlets.

Crop: Sugar Beet

Product: Delfan® and Aton AZ

Dosage: 1,5 L Delfan and 2,5 L Aton AZ / ha at 8-10 leaves

stage; foliar application

Contractor: Lithuanian University of Agriculture, Lithuania

Parameters assessed: Total and sugar Yield

Total Yield T/ha

+4,4%+2,3%

+8,5%

+3,6%

+4,1%+4,4%

+6,5%

+2,2%

40

45

50

55

60

65

70

75

80

85

90

2002 2003 2004 Average

Control

Delfan

Aton AZ

Sugar Yield T/ha

+5,7%+1,7%

+7,8%

+7,6%

+5,5%+6,5%

+3,5%

+6%

5

7

9

11

13

15

17

19

2002 2003 2004 Average

Delfan and Aton AZ improves significantly the total and the sugar yield of sugar beet during three years of trials. Delfan and Aton AZ are easy to use because them could be mixed with other fertilizers or plant protection products.

Crop: Summer Oilseed Rape

Product: Delfan® and Aton AZ

Dosage: 1,5 L Delfan and 2,5 L Aton AZ /ha at 3-4 leaves

stage; foliar application

Contractor: Lithuanian University of Agriculture, Lithuania

Parameters assessed: Yield and Quality parameters

Oilseed rape Yield - T/ha

+6,2%

+5%+11,1%

+3,4%

+5,4%

+1,4%+7,2%

+7,1%

1,5

1,7

1,9

2,1

2,3

2,5

2,7

2,9

2002 2003 2004 Average

Control

Aton AZ

Delfan

Delfan and Aton AZ improves significantly the yield of Summer Oilseed Rape during three years of trials. Delfan performed very well under stress conditions, 2002 was a very dry year with low production but the anti-stress effect of the amino acids allowed a big increase in yield.

Crop: Malt Barley

Product: Delfan®

Dosage: 1 L/ha at node stage; foliar application

Contractor: Lithuanian University of Agriculture, Lithuania

Parameters assessed: Yield and content of green proteins

Delfan improves significantly the yield of malt barley. Delfan performed very well under stress conditions, 2002 was a very dry year with low production but the anti-stress effect of the amino acids allowed a big increase in yield.

The content of green proteins was increased after application of Delfan: + 0,48%

Yield T/ha

+8,8%

3

3,2

3,4

3,6

3,8

4

4,2

4,4

Control

Delfan

Crop: Malt Barley

Product: Aton AZ

Dosage: 2 L/ha at node stage; foliar application

Contractor: Lithuanian University of Agriculture, Lithuania

Parameters assessed: Yield and content of green proteins

Yield T/ha

+11,4%

+11,5%+3,8%

+8,7%

3

3,5

4

4,5

5

5,5

6

2002 2003 2004 Average

Control

Aton AZ

Aton AZ improves significantly the yield of malt barley during three years of trials. Aton AZ performed very well under stress conditions, 2002 was a very dry year with low production but the anti-stress effect of the amino acids allowed a big increase in yield.

The content of green proteins was also measured after application of Aton AZ, it increased in +0,56%

Crop: Rose var. Forever Young

Product: Aton Ca

Dosage: 280 ml Aton Ca / 100 L of water (150 ppm of Ca)

One foliar application each week.

Contractor: Ecoroses Farm, Ecuador

Parameters assessed: Yield by categories, quality parameters

Yield results: Sorting by categories

29

209238 252

19

233

0

30

60

90

120

150

180

210

240

270

TOTAL EXPORT NATIONAL MARKET

nº

of

ste

ms

Control

Aton Ca (2,8 ml/l)

Number of stems by categories

Categories (cm) 60 70 80 90 100 120

Control 38 43 38 26 36 28

Aton Ca 22 20 16 42 99 33

The total production of stems and the % of stems to export increase with Aton Ca applications. In addition, the classification of stems by length shows that applications of Aton Ca increase the number of largest stems and reduce the amount of shortest stems.

Crop: Courgette var. Grey Zucchini

Product: Aton Mo

Dosage: 50-75-100 ml/100 L of water; 3 Foliar applications.

During vegetative development till flowering

Contractor: University Autonoma of Chapingo, Mexico

Parameters assessed: Yield, number of fruits, fresh plant weight

Yield Number of fruits

Aton Mo increases significantly the total number of fruits and yield. The dosage of 100 ml / 100 L of water is the most effective to improve courgette yield parameters. The plant fresh weight is also increased in the plants where Aton Mo was applied.In this trial was also stated that Aton Mo has no phytotoxic effect on leaves.

Effect of 3 different dosages of Aton Mo on courgette yield parameters

2.662.83

3.50

2.33

0

0.5

1

1.5

2

2.5

3

3.5

135.8

159.8

184.0

66.3

0

20

40

60

80

100

120

140

160

180

200

Aton Mo 50ml/100 ltagua

Aton Mo 75ml/100 ltagua

Aton Mo 100ml/100 ltagua

Testigo

Promedio

Aton Mo 50ml/100L

Aton Mo 75ml/100L

Aton Mo 100ml/100L

Control