dehydrogenase activity of soil microbial activity in soil 以去氫脢測量土壤微生物活性
Post on 20-Dec-2015
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Dehydrogenase Activity of Soil
Microbial activity in soil
以去氫脢測量土壤微生物活性
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秤 480 克土壤,並加入 4.8 克的碳酸鈣於塑膠袋中混合均勻。
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秤取 20.2 克的混合物加到試管中,每處理三重複。
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每試管皆加入 4 毫升 0.5% TTC 溶液, A 及 B 處理再分別加入 4 毫升的水或是 0.5% 的葡萄糖液。
A B
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輕拍管身,趕出氣泡,加上塞子,將試管置於 28℃,經 0, 1, 2, 3 天培養後,每處理取出三試管測 TPF 含量。
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經兩天培養後,清楚可見不同處理間產生 TPF 的不同,左為對照組,右為處理組。
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在抽器櫃內,每試管加入 10 毫升的甲醇。
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在震盪器上震盪 5 分鐘
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將混合物分裝至兩個尖底離心管內
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以桌上型離心機全速( 10000 rpm )離心 10 分鐘
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測試物經離心後的情形
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倒出上清液,讀取 485nm 的吸光值,以甲醇為校正液
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將所得吸光值帶入下列公式,求出 TPF 的產生量及氫的消耗量
Standard curve ( 0 – 0.06mg/mL)
Y = 1.867 + 52.134 ( X – 0.035 ) r 2= 0.992
產生 1mg TPF 需消耗 150.35 L H2
換算: L H2 / g 烘乾土