Biological control of Husk Spot

Prepared by: Dean Metcalf

Thursday 16 October 2014

MC12008:Biological control of Husk Spot.

Dean Metcalf

Collaborators:

Jeremy Bright

Steve McLean

Olufemi Akinsanmi

Robbie Commens

Trichoderma spp.

Botrytis cinerea

• .

Tissue sample direct plating.

94 Isolations

• Trichoderma x 50

• Gliocladium x 5

• Cladosporium x 2

• Fusarium x 3

• Epicoccum x 3

• 19 culled due to

inadequate

sporulation

• Penicillium x 4

• Aspergillus x 2

• Alternaria x 1

• Capnodium x 1

• Sporothrix x 2

Screening

Where the colonies meet…

Gliocladium MC94from a neglected plantation near Bundaberg

Collected by Eddie Dunne.

Trichoderma MC60Les Gain, near Gympie. Young leaves in A16 plantation.

Survival on husk ( / 10)LSD0.05 T=1.09 G=0.57

Assessing lesion invasion

Invasion of lesions ( /10) Lsd0.05 G=1.16, T=0.94

First assessment% of husks with spots

0 1 2 3

Untreated

MC21 T

MC26 G

MC29 G

MC32 T

MC60 T

MC90 T

MC94 G

2nd assessment confirmed by microscope

Second assessment of 100 fallen nuts per tree

LSD0.05=0.89

Agent Strength of

culture

antagonism

Ease to

manufacture

CFU in

prototype

X109

Survival on

husk

Invade

Lesion

First field

assessment

of 10

nuts/tree

Second field

assessment of

approx. 100

fallen nuts

Total

Untreated0.05 0.1 3% 1.69%

MC21 T *** ***** 2.75

****

3.3

***

2.7

**

0%

*****

0.34%

****26

MC26 G **** *** 1.2

***

7.6

*****

6.9

****

0%

*****

0.51%

****29

MC29 G **** *** 1.12

***

9.9

*****

9.1

*****

1%

****

0.00%

*****29

MC32 T *** ***** 4.51

*****

7.5

*****

2.1

**

2%

**

0.22%

*****27

MC60 T ***** ***** 1.96

***

4.0

***

2.2

**

1%

****

1.15%

**24

MC90 T ***** ***** 2.99

****

4.5

***

3.3

***

3%

*

0.00%

*****26

MC94 G **** *** 2.15

****

0.4

* ??

6.1

***

3%

*

0.61%

****20

Time it had a name…..

Macanizer !!!!

Key work areas for development

• Field trial of best 3-4 isolates.– MC21, MC29, MC32, MC90.

• Rate of application.

• Timing of application.

• Chemical compatibility.

• > Commercial launch.

Thanks• NSW DPI

• Brice Kaddatz

• Eddie Dunne

• Les Gain

• Bob Howard

• Andrew Woodford & OCP.

• Craig Maddox

• Justine Cox

• Brooke McLean

• Dr Diana Leemon

• Michael Powell ( & Macadamia Conservation Trust).

• Ben Callaghan and Horticulture Australia Ltd.