day 1 scientific tracks & abstracts · 8 males) by suction-assisted liposuction (sal) and...
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Biotechnology, Stem Cell and Molecular Diagnostics
Biotechnology 2018 | Stem Cell 2018 | Molecular Diagnostics 2018
A p r i l 1 6 - 1 7 , 2 0 1 8 A m s t e r d a m , N e t h e r l a n d s
E u r o S c i C o n J o i n t E v e n t o n
Session Introduction
Day 1A p r i l 1 6 - 1 7 , 2 0 1 8
S e s s i o n sMedical Biotechnology | Bioproducts and BioEnergy | Bioremediation and Biodegradation, Advancements in Biotechnology | Bioproducts and BioEnergy | Nanobiotechnology | Clinical Biochemistry Novel stem cell technologies | Cancer Stem Cells | Stem Cell Product Development & Comercialization | Veterinary Stem Cell | Tissue Regeneration & Therapeutics | Stem Cell Banking
Title: Stromal Vascular Fraction from Lipoaspirate InfranatantRobert Bowen, West Virginia University, USA
Title: Biotechnology of tumor growth restriction: M3 macrophage “switch” phenotype and antigen-reprogrammed lymphocytesMalyshev Igor, Moscow State University of Medicine and Dentistry, Russia
Title: Production of Biofuels by Micro-, Lab-, Pilot- and Industrial Scale Based Biocatalytic ProcessesBruno Zelic, University of Zagreb, Croatia
Title: Inclusion bodies – more than just waste!Oliver Spadiut, Technische Universitat Wien, Austria
Title: A study on Subclinical hypothyroidism and association with Vitamin D in ethnic population of Kashmir IndiaHaamid Bashir, Govt Medical College, India
Title: About New Approaches for Enhancing Phytoremediation EffectivenessTamar Varazi, Agricultural University, Georgia
Title: The effects of acrylamide and Vitamin E administration during pregnancy on adult rat ovary tissuesZeliha Selamoglu, Nigde Omer Halisdemir University, Turkey
Title: Paper mill sludge as a good substrate for enzyme production by oyster mushroom (Pleurotus ostreatus)Mija Sezun, Pulp and Paper Institute, Slovenia
Title: A Novel Aptamer- Conjugated Gold Nanoparticle for reliable cancer cell detections Mojgan Ahmadzadeh Raji, University of Tehran, Iran
Title: The charge effect of insulin PLGA nanoparticles on cell uptake in vitro and bioavailability in vivoElodie Czuba, University of Strasbourg, France
Title: Analysis of different MSC tissue sources in horsesMagdalena Arevalo Turrubiarte, Department of Veterinary Science University of Turin Largo Braccini, Italy
Title: Induced pluripotent stem cells derived from porcine Sertoli cellsPiyathip Setthawong, Chulalongkorn University, Thailand
Title: Non-cytotoxic, non-genotoxic and non-mutagenic phenolic extract of cyanobacteria Oscillatoria sp. SI-SA with chemotherapeutic properties against breast cancer cell-linesSaadia Ijaz, Women University Multan, Pakistan
Session Chair Zeliha Selamoglu, Nigde Omer Halisdemir
University,Turkey
Session Co-ChairUtku AVCI, Recep Tayyip Erdogan
University, Turkey
E u r o S c i C o n J o i n t E v e n t o n
Biotechnology, Stem Cell and
Molecular Diagnostics
Biotechnology 2018 | Stem Cell 2018 | Molecular Diagnostics 2018Biochemistry & Molecular Biology JournalISSN 2471-8084
A p r i l 1 6 - 1 7 , 2 0 1 8 A m s t e r d a m , N e t h e r l a n d s
Page 41
Introduction: Lipoaspirate has shown great promise as a source of progenitor cells for use in regenerative medicine. The stromal vascular fraction (SVF) can be isolated from lipoaspirate using enzyme digestion and centrifugation, but this approach may be limited by the labour-intensive nature of the technique as well as ambiguities in current governmental regulations. An alternative approach to obtain SVF from lipoaspirate was studied.
Methodology: Paired (collected from contralateral regions) lipoaspirate specimens were acquired from 30 consenting patients (age 24-62; 22 females, 8 males) by suction-assisted liposuction (SAL) and nutational infrasonic liposuction (NIL). The infranatant from 50 ml of adipose tissue (LAF) was centrifuged at 400 g×5 min and the resultant pellet was collected with a pipette. Time=15-20 min. The respective SVFs cell populations were counted using an optical fluorescent cell counter (Nexcelom A2000) and the fluorescent stains-acridine orange (AO) and propidium iodide (PI).
Results: The number of nucleated, live cells from SAL infranatant was 97,345±23,435 per ml of adipose tissue and from NIL infranatant was 335,621±81,274 per ml of adipose tissue. The p value is <0.00001, n=30.
Conclusions: Regenerative cells can be isolated from the lipoaspirate infranatant from either SAL or NIL, although in lower quantities than from enzyme digestion. NIL acquisition yielded 3.5×the number of cells over that acquired from SAL. The time, skill, and cost of producing SVF from infranatant is less than using enzyme digestion, which potentially make these regenerative therapies accessible to more physicians and patients.
BiographyRobert Bowen graduated from the University of Illinois, School of Medicine and trained in internal medicine, critical care, and pulmonary medicine at the University of Virginia and West Vir-ginia University (WVU) medical centers. He was trained in Laser Bronchoscopy in 1982/1983 and after completing a Fellowship in cosmetic anti-aging and regenerative medicine extended his laser experience to include aesthetic therapies. He is currently the Professor of Medicine at WVU and Director of the Center for Wound Care and Hyperbaric Medicine at WVU Berkeley Medical Center, USA. His current research interests include the transla-tion of adipose derived stem cell treatments and light-based therapies into clinical applications in wound care and cosmetic medicine.
Stromal vascular fraction from lipoaspirate infranatantRobert BowenWest Virginia University School of Medicine, USA
Robert Bowen, Biochem Mol biol J 2018 Volume: 4DOI: 10.21767/2471-8084-C2-011
E u r o S c i C o n J o i n t E v e n t o n
Biotechnology, Stem Cell and
Molecular Diagnostics
Biotechnology 2018 | Stem Cell 2018 | Molecular Diagnostics 2018Biochemistry & Molecular Biology JournalISSN 2471-8084
A p r i l 1 6 - 1 7 , 2 0 1 8 A m s t e r d a m , N e t h e r l a n d s
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Many tumors produce anti-inflammatory cytokines, which reprogram antitumor M1 macrophages to protumor M2 macrophages via activation
of transcription factors, STAT3, STAT6, and SMAD3. Earlier we showed that М1 macrophages with inhibited STAT3, STAT6, and SMAD3 (M3 phenotype) responded to the action of protumor, anti-inflammatory cytokines by increasing production of antitumor, proinflammatory cytokines and thus, preserved their antitumor properties. In vivo, the tumor also disorders the antigen presentation by macrophages and prevents formation of antigen-specific Т and Th1 lymphocytes with strong antitumor properties. We hypothesized that presentation of tumor antigens to lymphocytes by M3 macrophages in vitro, in absence of tumor cells, could result in an effective antitumor programing of the lymphocytes. It can be expected that a composite pool of M3 macrophages and in vitro antigen-reprogramed lymphocytes would effectively restrict tumor growth. We showed that the antitumor effect of M3 macrophages depended on timing of their administration following the onset of solid tumor development. In early administration, M3 macrophages partially restricted the tumor development. In late administration, M3 macrophages restricted the tumor growth but to a significantly less extent. Adding antigen-reprogramed lymphocytes to M3 macrophages resulted in complete inhibition of tumor growth both in vitro and in vivo, both in early and late administration. The fact that M3 macrophages and antigen-reprogramed lymphocytes completely suppressed tumor growth makes it very promising to develop a clinical biotechnology for reducing the tumor growth by prior in vitro antitumor programing of the immune response.
BiographyMalyshev Igor is a Head of the Department of Pathophysiolo-gy and Head of the Laboratory of Cell Biotechnology, Medical School at the Moscow State University of Medicine and Den-tistry; 2. Head of the Laboratory of Stress, Institute of General Pathology and Pathophysiology, Moscow and 3. Adjunct Pro-fessor of Biomedical Sciences, University of North Texas Health Science Center, USA. He is a Member of the board of directors of the International Society for Adaptive Medicine and an Edi-torial board member of Journal of Biosciences and Medicines. He has published 3 books and monographs and 153 full length articles. Research Interests: immunity, cancer, stress and adap-tation
Biotechnology of tumor growth restriction: M3 macrophage “switch” phenotype and antigen-reprogrammed lymphocytesMalyshev IgorMoscow State University of Medicine and Dentistry named A.I. Evdokimov, Russia
Malyshev Igor, Biochem Mol biol J 2018 Volume: 4DOI: 10.21767/2471-8084-C2-011
E u r o S c i C o n J o i n t E v e n t o n
Biotechnology, Stem Cell and
Molecular Diagnostics
Biotechnology 2018 | Stem Cell 2018 | Molecular Diagnostics 2018Biochemistry & Molecular Biology JournalISSN 2471-8084
A p r i l 1 6 - 1 7 , 2 0 1 8 A m s t e r d a m , N e t h e r l a n d s
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The need for the production of biofuels from various renewable sources is becoming increasingly interesting especially as the availability and
accessibility of fossil fuels is significantly declining. Biodegradability, low pollution emissions and non-toxicity of raw materials are some properties making biogas, biodiesel and bioethanol more environmentally friendly fuels.
Solid-state fermentation could be a suitable technology for the production of value-added products by utilization of the renewable waste materials, which makes it also economically feasible. So far, this technology was used for production of enzymes, organic acids, mushrooms, flavour and aroma compounds, pigments, polysaccharides, hormones, human food and animal feed. Different type of bioreactors have been developed and successfully used for solid-state fermentation of broad range of substrates and in production of value-added products. Solid-state fermentation will be demonstrated as part of anaerobic degradation on lab-, pilot- and industrial-scale of several waste materials such as brewer's spent grain, whey and cow manure, and corn silage and cow manure, respectively.
The application of different microreactor systems in intensification of the biodiesel production process is widely studied. However, previous studies of the application of microreactor technology in the production of biodiesel were limited to the use of chemical catalysts. Mild reaction conditions, absence of by-products, reusability, simple separation and purification of the resulting biodiesel as well as lower energy consumption are some of the many advantages that make the enzyme lipase – a biocatalyst – a better choice than traditional chemical catalysts in the process of biodiesel production. Different microreactor systems utilising a commercially available lipase and a lipase produced by solid-state fermentation were used for transesterification of fresh and waste cooking oil while biodiesel was separated using integrated microseparation unit.
Selected examples are clear demonstration of environmentally friendly and economic technologies used for efficient production of biofuels on micro-, lab-, pilot- and industrial scale.
Keywords—anaerobic digestion, biofuels, microreactors, solid-state fermentation
BiographyBruno Zelic received his PhD in chemical engineering in 2003 at University of Zagreb. From 2012 he is full professor at the Faculty of Chemical Engineering and Technology, University of Zagreb. His research interests are in the field of implementation of microreactor technology into biotechnology and biofuels pro-duction. More than 80 scientific and professional publications, 2 patents, and more than 60 oral and poster presentations on the international conferences present his scientific work. Bru-no Zelic was dean of the Faculty of Chemical Engineering and Technology, University of Zagreb from 2013 to 2017. He is Edi-tor-in-Chief of Chemical and Biochemical Engineering Quarterly journal.
Production of Biofuels by Micro-, Lab-, Pilot- and Industrial Scale Based Biocatalytic ProcessesBruno ZelicUniversity of Zagreb, Faculty of Chemical Engineering and Technology, Croatia
Bruno Zelic, Biochem Mol biol J 2018 Volume: 4DOI: 10.21767/2471-8084-C2-011
E u r o S c i C o n J o i n t E v e n t o n
Biotechnology, Stem Cell and
Molecular Diagnostics
Biotechnology 2018 | Stem Cell 2018 | Molecular Diagnostics 2018Biochemistry & Molecular Biology JournalISSN 2471-8084
A p r i l 1 6 - 1 7 , 2 0 1 8 A m s t e r d a m , N e t h e r l a n d s
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Against the outdated belief that inclusion bodies (IBs) in Escherichia coli are only inactive aggregates of misfolded protein, and thus should be avoided during recombinant protein production, numerous biopharmaceutically important proteins are currently produced as IBs. To obtain correctly folded, soluble product, IBs have to be processed, namely harvested, solubilized and refolded. Several years ago, it was discovered that, depending on cultivation conditions and protein properties, IBs contain partially correctly folded protein structures, which makes IB processing more efficient. I present a method of tailored induction of recombinant protein production in E. coli by a mixed feed system using glucose and lactose and its impact on IB formation. My method allows tuning of IB amount, IB size, size distribution and purity, which does not only facilitate IB processing, but is also crucial for potential direct applications of IBs as nanomaterials and biomaterials in regenerative medicine.
Keywords— Escherichia coli BL21(DE3), inclusion body, pET expression system, lactose
BiographyOliver Spadiut studied Food Science and Biotechnology at the University of Natural Resources and Life Sciences (Vienna). After 18 months of successful post-doctoral research at the Royal University of Technology KTH in Stockholm, Sweden, he became University Assistant at TU Wien, Austria. Dr. Spadiut received his Habilitation in Biotechnology in March 2015 and is currently Asst. Prof. at TU Wien and group leader of the re-search group "Integrated Bioprocess Development".
Inclusion bodies – more than just waste!Oliver SpadiutResearch Division Biochemical Engineering, Institute of Chemical, Environmental and Biological Engineering, Austria
Oliver Spadiut, Biochem Mol biol J 2018 Volume: 4DOI: 10.21767/2471-8084-C2-011
E u r o S c i C o n J o i n t E v e n t o n
Biotechnology, Stem Cell and
Molecular Diagnostics
Biotechnology 2018 | Stem Cell 2018 | Molecular Diagnostics 2018Biochemistry & Molecular Biology JournalISSN 2471-8084
A p r i l 1 6 - 1 7 , 2 0 1 8 A m s t e r d a m , N e t h e r l a n d s
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Background: Subclinical hypothyroidism is defined as elevated serum TSH levels with normal levels of T3 and T4 hormones. Vitamin D is an important vitamin that not only regulates calcium, but also has many other beneficial actions like immune modulator. The aim of study is to find the relationship between Subclinical hypothyroidism and vitamin D levels in ethnic population of Kashmir, India.
Subjects and Methods: Thyroid hormones (FT3, FT4, TSH, T3, T4, Anti thyroid peroxidases) and vitamin D (25-OH) levels were measured in 80 patients with Subclinical hypothyroidism and 70 healthy subjects, by Chemiluminisence method on Abbott USA I 1000SR Automatic immunoassay analyzer in Department of Biochemistry, Govt Medical College, Srinagar.. Vitamin D deficiency was designated at levels lower than 30 ng/ml. Calcium levels were also evaluated in all participants on biochemistry analyzer Abbott USA c4000.
Results: Serum 25(OH) vit D was significantly lower in Subclinical hypothyroid patients than in healthy controls (F=27.7, P<0.0001). Its level was significantly decreased in females than male patients (t=1.2, P<0.05).Calcium levels was also a significant decrease in subclinical hypothyroid patients when compared to controls (t=1.5, P<0.05).
Conclusion: On the basis of present study in mountainous valley of Kashmir subclinical hypothyroid patients are suffered from hypovitaminosis D and hypocalcaemia that is significantly associated with the degree and severity of the Subclinical hypothyroidism. Thus we suggest that patients are to be encouraged for vit D supplementation and we recommend that the mass screening for Vitamin D, Thyroid hormone profile and serum calcium levels in this ethnic population of Kashmir.
Keywords: Vit D deficiency, Blood calcium levels, subclinical hypothyroidism, Kashmir.
BiographyHaamid Bashir is pursuing Doctorate (PhD) in Biological Sci-ence from University of Kashmir, Hazratbal Srinagar through Research centre Department of Biochemistry, Government Medical College Srinagar. Haamid has published more than 15PubMed indexed publications in reputed national and inter-national journals. One of publication is published in European cancer and prevention journal (impact factor 3.5). Haamid has expertise in molecular genetics and epigenetics in cancer biolo-gy, Thyroid, Diabetes, Metabolic syndrome. Haamid has attend-ed many national conferences and workshops. Haamid has been associated with number of scientific journals.
A study on Subclinical hypothyroidism and association with Vitamin D in ethnic population of Kashmir IndiaHaamid Bashir1, Sabhiya Majid1*, Rakesh Kumar Koul2, Rabia Hamid3, Rabia Farooq1 Inshah Din1, Sheikh Ishaq1.1Department of Biochemistry, Govt Medical College, Srinagar, 2Department of Medicine, Govt Medical College, Srinagar, 3Department of Biochemistry, University of Kashmir.
Haamid Bashir et.al, Biochem Mol biol J 2018 Volume: 4DOI: 10.21767/2471-8084-C2-011
E u r o S c i C o n J o i n t E v e n t o n
Biotechnology, Stem Cell and
Molecular Diagnostics
Biotechnology 2018 | Stem Cell 2018 | Molecular Diagnostics 2018Biochemistry & Molecular Biology JournalISSN 2471-8084
A p r i l 1 6 - 1 7 , 2 0 1 8 A m s t e r d a m , N e t h e r l a n d s
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The complex technological approach to the fast removal of ecotoxicants from ecosystems is a very urgent matter for all countries. The development
of water remediation methods for the removal of chemical contaminants is a challenging problem. One of the methods to clean chemically polluted waters is using of algae (the so-called Phycoremediation). Spirulina (Spirulina platensis) should have prospects for phytoremediation of waters polluted by different toxic compounds. Evaluation of ecological potential of Spirulina, in particular, its tolerance and detoxication ability towards organic ecotoxicants and heavy metals, is the novelty in sphere of researches in xenobiochemistry.
Recently intensive study of contaminated areas has revealed special property of bacteria living in these areas. The processes of microorganism’s biostabilization of soluble forms of hazardous pollutants can be used for bioremediation of soils, contaminated by oil, Pesticides, heavy metals and radionuclides. Monitoring of bacterial consortiums in contaminated regions will enable to assess in advance the extent of native bioremediation and accordingly suggest a strategy of detoxification. For this purpose we have developed the phylogenetic oligonucleotide low-density biochip based on the 16SrRNA genes sequences.
The goal of presented work is to develop of quick response strategy and effective flexible technology of targeted toxicants removal from polluted water and soils. The approach is based on joint action of microorganism and plants with high detoxification potential, using natural minerals with function of a sorbent is to uptake and to trap pollutants emission in the environment and biochips – tool for bioaugmentation of different type of toxicants.
BiographyTamar Varazi‘s qualification - chemist - complated at Tbilisi State University of Georgia. She received her scientific degree – PhD in Biochemistry at the Institute of Biochemistry and Bio-technology of Georgia.Current ocupation is Senior scientist and Full Professor of Georgian Agricultural University, Durmishidze Institute of Biochemistry and Biotechnology, Department of Bi-ological Oxidation. She has scientific experiences in Investiga-tion of biochemical principles of phytoremediation of polluted environment. She is a manager, coordinator and researcher of many scientific and applied international projects. She has highly developed presentation/teaching skills. Her publications includes 3 Monographs and 47 articles in international and lo-cal scientific journals.
About New Approaches for Enhancing Phytoremediation EffectivenessTamar VaraziAgricultural University of Georgia, Durmishidze Institute of Biochemistry and Biotechnology, Department of Biological Oxidation, Georgia
Tamar Varazi, Biochem Mol biol J 2018 Volume: 4DOI: 10.21767/2471-8084-C2-011
E u r o S c i C o n J o i n t E v e n t o n
Biotechnology, Stem Cell and
Molecular Diagnostics
Biotechnology 2018 | Stem Cell 2018 | Molecular Diagnostics 2018Biochemistry & Molecular Biology JournalISSN 2471-8084
A p r i l 1 6 - 1 7 , 2 0 1 8 A m s t e r d a m , N e t h e r l a n d s
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To investigate changes in adult female rat ovary tissue following acrylamide (AA) and vitamin E administration during pregnancy. The present study was
conducted with the approval of the experimental animals ethics committee at Inonu University, Faculty of Medicine (2017 / A-11). Thirty rats, confirmed to be pregnant with vaginal smear, were divided into 5 different groups that included equal number of pregnant rats: Control, Corn oil, Vitamin E, Acrylamide, Vitamin E + Acrylamide Groups. The birth was monitored on the 21st day of gestation and female rats were selected and at the end of 8 weeks, the rats were decapitated. Rat ovary tissues were examined for malondialdehyde (MDA), reduced glutathione (GSH), total antioxidant status (TAS), total oxidant status (TOS) and superoxide dismutase (SOD), catalase (CAT) and nitric oxide (NO) levels. It was determined that AA had a negative effect on oxidant-antioxidant parameters (MDA, GSH, NO, SOD, CAT, TAS, TOS) in the ovary tissue (P<0.05), whereas Vitamin E administration increased GSH, TAS, NO, SOD, CAT levels (P<0.05). It is unlikely to be exposed to food-borne AA toxicity and AA toxicity could lead to permanent damages. It is certain that infertility incidences are increasing among the population every day. Vitamin E is observed to have protective effects against AA toxicity, however further studies are required.
Keywords: Pregnancy, acrylamide, Vitamin E, ovary, oxidative stress.
BiographyZeliha Selamoglu is a Professor in Medical Biology department of Nigde Omer Halisdemir University, Turkey. She earned her PhD in Biology from Inonu University, She has published over 90 peerreviewed journal articles with over 760 citations and many technical reports. She is a member of Society for Experimental Biology and Medicine: Associate Membership and European as-sociation for cancer research. She has served as Editorial Board member for many Journals.
The effects of acrylamide and Vitamin E administration during pregnancy on adult rat ovary tissuesMehmet Erman Erdemli1, Zeliha Selamoglu2, Yusuf Turkoz3
1Department of Medical Biochemistry, Faculty of Medicine, Nigde Ömer Halisdemir University, Nigde, Turkey 2Department of Medical Biology, Faculty of Medicine, Nigde Ömer Halisdemir University, Nigde, Turkey 3Department of Medical Biochemistry, Faculty of Medicine, Inonu University, Malatya, Turkey
Zeliha Selamoglu et al., Biochem Mol biol J 2018 Volume: 4DOI: 10.21767/2471-8084-C2-011
E u r o S c i C o n J o i n t E v e n t o n
Biotechnology, Stem Cell and
Molecular Diagnostics
Biotechnology 2018 | Stem Cell 2018 | Molecular Diagnostics 2018Biochemistry & Molecular Biology JournalISSN 2471-8084
A p r i l 1 6 - 1 7 , 2 0 1 8 A m s t e r d a m , N e t h e r l a n d s
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The largest by-product in pulp and paper industry is sludge and disposal is major solid waste problem for the industry (Battaglia et al., 2003; Geng
et al., 2006). It was predicted that global production of paper mill sludge rise over the 50 years by between 48 and 86% over current levels (Mabee & Roy, 2003). The landfilling of waste paper mill sludge has become less achievable in recent years as environmental concerns have lead to rapidly increasing costs. Sanchez (2010) report that the mushrooms have economical, ecological and medicinal values. Their advantage is also that they are able to colonize and degrade a large variety of lignocellulosic materials and other wastes which are produced in agricultural, forest and food-processing industries. We studied the growth of oyster mushroom (Pleurotus ostreatus) on pulp and paper industry solid wastes. During the study, we investigated whether solid-state fermentation and growth substrates (de-inking paper mill sludge, primary sludge) are appropriate for P. ostreatus production of enzymes relevant to the pulp and paper industry. Following fermentation, extracellular protein was extracted and the specific activities of four enzymes were determined: the cellulase, xylanase, lipase, and peroxidase activities. These enzymes are used in the paper industry for the improvement of various industrial processes and in many other applications. Results show that P. ostreatus can grow on pulp and paper industry solid wastes, which will help to minimize the waste volume, and to decrease the ecological impact. Furthermore, these pulp and paper industry solid wastes are good substrates for the production of commercially interesting enzymes.
BiographyMija Sezun has completed her PhD on biological and biotechno-logical sciences. Her doctoral thesis included the environmental biotechnology area. At the moment she is working at the Pulp and Paper Institute and mainly deals with biotechnology in the paper industry through the use of enzymes in the process of paper production. Currently researching the production of enzymes by using fungi by applying paper mill sludge, as the substrate for the cultivation of fungi. In addition to the fungal enzyme production she also deals with the use of commercial enzymes to improve the efficiency of processes in the paper industry.
Paper mill sludge as a good substrate for enzyme production by oyster mushroom (Pleurotus ostreatus)Mija Sezun, Matej Skocaj, Andrej Gregori, Kristina Sepcic Pulp and Paper Institute, Slovenia
Mija Sezun et al., Biochem Mol biol J 2018 Volume: 4DOI: 10.21767/2471-8084-C2-011
Biotechnology 2018 | Stem Cell 2018 | Molecular Diagnostics 2018
E u r o S c i C o n J o i n t E v e n t o n
A p r i l 1 6 - 1 7 , 2 0 1 8 A m s t e r d a m , N e t h e r l a n d s
Biotechnology, Stem Cell and Molecular Diagnostics
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E u r o S c i C o n J o i n t E v e n t o n
Biotechnology, Stem Cell and
Molecular Diagnostics
Biotechnology 2018 | Stem Cell 2018 | Molecular Diagnostics 2018Biochemistry & Molecular Biology JournalISSN 2471-8084
A p r i l 1 6 - 1 7 , 2 0 1 8 A m s t e r d a m , N e t h e r l a n d s
Page 51
Introduction: Poly (D, lactic-co-glycolic) acid (PLGA) nanoparticles (NP) are known to be effective drug carrier with a long acting profile but have less than 20% of bioavailability.
Aim: The aim of this work was to increase encapsulated insulin bioavailability modifying the surface properties like charge of NPs to improve their interaction with cell membrane.
Materials & Methodology: Particles were prepared in presence or not of polyvinyl alcohol (PVA), with Sodium dodecyl sulfate (SDS) or coated with chitosan chloride to play on NPs charge. In vitro internalization was tested on epithelial coculture (Caco-2/RevHT29MTX) by flow cytometry. NPs were then administrated thanks to a pharmaceutical complex vector in vivo by oral route (10, 20 and 50 UI) in diabetic rats.
Results: SDS-NPs and 1% PVA-NPs were smaller (141±3 and 154±24 nm) than chitosan coated NPs with size (236±29 nm) which is increased in comparison to the control (200±9 nm). Compared to classical NPs (PVA+), cells uptake was improved by SDS-NPs, in contrast to without PVA and chitosan coated NPs. Administration by oral route of vehicle contained SDS-NPs 20 and 50 UI) reduced glycaemia faster than empty vehicles and vehicles with standard NPs. Negative charge of NPs could interact with cell membrane which is negatively charged too.
Conclusions: Mucoadhesive particles (chitosan) formulation is definitely not the good approach to improve the bioavailability of encapsulated insulin contrary to negative NPs are the most efficient both in vitro and in vivo and represent a promising formulation for oral insulin delivery.
BiographyElodie Czuba is a PhD student in European Diabetes study cen-ter. She is interested in nanoencapsulation of drugs for a bio-logical application
The charge effect of insulin PLGA nanoparticles on cell uptake in vitro and bioavailability in vivoElodie CzubaUMR DIATHEC, France
Elodie Czuba, Biochem Mol biol J 2018 Volume: 4DOI: 10.21767/2471-8084-C2-011
E u r o S c i C o n J o i n t E v e n t o n
Biotechnology, Stem Cell and
Molecular Diagnostics
Biotechnology 2018 | Stem Cell 2018 | Molecular Diagnostics 2018Biochemistry & Molecular Biology JournalISSN 2471-8084
A p r i l 1 6 - 1 7 , 2 0 1 8 A m s t e r d a m , N e t h e r l a n d s
Page 52
Mesenchymal cells (MSCs) have been used in regenerative medicine as an option to restore damaged tissues mostly in locomotory equine problems.
MSCs can be obtained from different tissues in equines. Previous studies have shown that source sites remain decisive for MSCs yield, cell viability and might influence cell behaviour in vitro and in vivo. To our knowledge, other studies have used cells from bone marrow, adipose tissue, and synovial fluid. However, an in-depth study from this different type of sources and a comparison among them in respect to MSCs in vitro performance and phenotype is lacking. The aim of this study was to evaluate through flow cytometry cell phenotype by cell surface markers (CD11α/CD18, CD45, CD79α, CD90, CD105 and MHC II) reported in equine MSCs. We completed this study with in vitro functional differentiation assays to confirm their mesenchymal identity. The evaluation of cell in vitro performance by different test (cell growth and migration) was made. We observed differences in tissue sources such as cells isolated from synovial fluid had a higher growth rate than bone marrow and adipose cells. Cell populations showed differences in the expression of surface markers, thus giving information about specific cell phenotypes. Adipose tissues had a highest expression of CD105 than bone marrow and synovial fluid cells. Adipose and bone marrow seem to be the main tissues used to obtain MSCs, however, synovial fluid cells have a closer chondrocytelike profile which might be a better option to specific joint issues. Among the different type of adipose cells, neck fat tissue seems to be a more feasible choice to obtain MSCs in addition to the amount of cells and cell performance characteristics. This study should help in the selection of a more appropriate tissue harvesting source in the use of MSCs for cell therapy in equines.
BiographyMagdalena Arevalo Turrubiarte has a bachelor in veterinary medicine, she obtained her MSc in Animal Production and Health at the Universidad Nacional Autonoma de México (UNAM) focusing on adipogenesis in beef cattle. She com-pleted her PhD at the Agrocampus Ouest-INRA, France with specialization in biology and agronomy working epithelial cell phenotype in the mammary gland bovine during a lactation pe-riod. Currently, she is working with the group of Veterinary Phys-iology at University of Turin in Italy in a project with the use of stem cells in tissue regeneration in equines. She has done farm practice and some laboratories internships in Mexico and in the United States of America
Analysis of different MSC tissue sources in horses Arevalo Turrubiarte M, Baratta M and Martignani E University of Turin, Italy
Arevalo Turrubiarte M et al., Biochem Mol biol J 2018 Volume: 4DOI: 10.21767/2471-8084-C2-011
E u r o S c i C o n J o i n t E v e n t o n
Biotechnology, Stem Cell and
Molecular Diagnostics
Biotechnology 2018 | Stem Cell 2018 | Molecular Diagnostics 2018Biochemistry & Molecular Biology JournalISSN 2471-8084
A p r i l 1 6 - 1 7 , 2 0 1 8 A m s t e r d a m , N e t h e r l a n d s
Page 53
Induced pluripotent stem cells (iPSCs) are generated by reprogramming of fully differentiated adult cells using four transcription factors, including
OCT4, SOX2, KLF-4 and c-MYC (OSKM). Porcine is a meaningful model for regenerative medicine because its anatomy and physiology are similar to human. However, reprogramming efficiency of porcine fibroblasts into iPSCs is currently poor. This study used Sertoli cells as a novel cell origin for somatic cells reprogramming. Neonatal testes were collected from 1-week old piglets. The testes were digested by two-step enzymatic method in order to isolate the Sertoli cells. The Sertoli cells were transfected with retroviral vectors expressing OSKM. We observed the primary colonies and counted on day 7 after transfection. The characteristics of Sertoli iPSCs-like colonies were analyzed by morphology, alkaline phosphatase staining, RT-PCR, G-banding, in vitro and in vivo differentiation. Sertoli cells obtained from neonatal porcine showed typically polygonal shaped. A total of 240 colonies (0.33%) originated from seeding 72,500 cells were observed on day 7. The Sertoli iPSCs-like colonies exhibited a high nuclear per cytoplasm ratio with prominent nucleoli. We picked up 30 Sertoli iPSCs-like colonies and 8 cell lines (26.6%) demonstrated undifferentiated stage of iPSCs. The Sertoli iPSCs-like colonies were positive to alkaline phosphatase staining and expressed endogenous pluripotent. G-banding analysis demonstrated normal karyotype. Under differentiation conditions, iPS-like cell lines could form three-dimension aggregated masses, which represented three germ layers of embryonic cells. For in vivo differentiation, tumour mass were collected and presented all of ectoderm, mesoderm, and endoderm. In conclusion, the Sertoli cells can be used as a novel somatic cell origin for iPSCs reprogramming.
BiographyP Setthawong has completed her Bachelor's Degree in Faculty of Veterinary Science (First-Class Honors) from Chulalongkorn University, Thailand. She got a Scholarship in Thailand Research Fund through the Royal Golden Jubilee PhD Program (Grant No. PHD/0143/2556). She is currently pursuing a Doctor of Philos-ophy Degree in the field of Obstetrics, Gynaecology and Repro-duction at Chulalongkorn University, Thailand. She is interested in veterinary stem cells especially in pig as an animal model for human. She aims to study about generation of embryonic stem cells and induced pluripotent stem cells. She has succeeded in establishing and characterizing induced pluripotent stem cells derived from Sertoli and fibroblast cells.
Induced pluripotent stem cells derived from porcine Sertoli cellsP Setthawong1, P Phakdeedindan1, N Tiptanavattana2, S Run-garunlert3, M Techakumphu1 and T Tharasanit11Chulalongkorn University, Thailand2Prince of Songkla University, Hat Yai, Thailand3Mahidol University, Nakhon Pathom, Thailand
P Setthawong et al., Biochem Mol biol J 2018 Volume: 4DOI: 10.21767/2471-8084-C2-011
E u r o S c i C o n J o i n t E v e n t o n
Biotechnology, Stem Cell and
Molecular Diagnostics
Biotechnology 2018 | Stem Cell 2018 | Molecular Diagnostics 2018Biochemistry & Molecular Biology JournalISSN 2471-8084
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The 70% methanolic extract of Cyanobacterial strain; Oscillatoria sp. SI-SA containing phenolic compounds; Tannic acid, Orcinol, Pholoroglucinol,
Salicylic acid, Acetyl Salicylic acid and Protocatechuic acid, was evaluated for its non-cytotoxic, non-genotoxic and non-mutagenic activities along with its anticancer potential against two (MCF-7 and MDA-MB-231) breast cancer cell-lines in order to study their chemotherapeutic effects without causing damage to normal cells. The strain was isolated from Kallar Kahar Salt Lake, Pakistan. It was identified by polyphasic approach including both morphological and molecular methodologies. The extract showed very low cytotoxicity against normal lymphocytes only at highest concentration of 1000 µg/ml with high IC50 value of 1088 µg/ml. Comet assay also showed low genotoxicity only at highest non-cytotoxic concentration of 1000 µg/ml with 15.37% DNA damage. Ames fluctuation Salmonella typhimurium test against four mutant strains TA100, TA98, TA97a and TA102 also showed significant non-mutagenic effects of the extract with and without metabolic activation. Finally, the extract was evaluated for its anticancer potential which showed significant anticancer activities in dose-dependent manner against MCF-7 and MDA-MB-231 cell-lines giving low IC50 values of 61.75 and 82.75 µg/ml respectively. It was further observed that after three days of treatment with the extract, 19.29 and 26.81 % cell viability remained of MCF-7 and MDA-MB-231 cell-lines respectively at highest concentration of 250 µg/ml. The phenolic extract of Oscillatoria sp. SI-SA indeed showed promising anticancer activities without causing severe damage to normal cells and thus could be used as an alternative bioresource for anticancer therapeutics against breast cancers.
BiographySaadia Ijaz is a Young Researcher. She has just completed her PhD in April, 2017 from The University of the Punjab, Lahore Pa-kistan. Currently she is working as Assistant Professor in The Women University, Multan Pakistan. Her main research interest is in the role of Cyanobacteria in biotechnology including but not limited to nutraceutical, pharmaceutical, biofuel production, environmental biosensors, cosmetics, biomedical research ap-plications and harmful aspects of cyanobacteria. She has pub-lished 2 research papers in impact factor journals and also won Rs. 0.5 million Start-up research grant from Higher Education Comission of Pakistan for her work with anticancer carotenoids from Cyanobacteria.
Non-cytotoxic, non-genotoxic and non-mutagenic phenolic extract of cyanobacteria Oscillatoria sp. SI-SA with chemotherapeutic properties against breast cancer cell-linesSaadia Ijaz1, 2* and Shahida Hasnain1, 21Department of Microbiology and Molecular Genetics, University of the Punjab, Quaid-e-Azam Campus, Lahore 54590, Pakistan.2The Women University, Multan, 60000, Punjab, Pakistan.
Saadia Ijaz et al., Biochem Mol biol J 2018 Volume: 4DOI: 10.21767/2471-8084-C2-011
Biotechnology 2018 | Stem Cell 2018 | Molecular Diagnostics 2018
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This WORKSHOP will review the science behind PRP and stem cell injections and their clinical use in Sports Medicine and Regenerative Medicine. We will
explain the harvesting and preparation of those injections, talk about their clinical indications for injuries, osteoarthritis, cosmetic use and male/female health function. Clinical results will be discussed and the presentation will end with open discussion with the audience. Dr Robert currently uses PRP and stem cell injections to treat medical issues such as cartilage damage, tendon and ligament tears and cosmetic issues including; stretch marks, hair loss, acne scarring, facial wrinkles and skin texture. In addition to the above Dr Robert also provides treatment for ED and female dysfunction using the same method. Dr Robert will discuss relevant cases under her treatment and share experiences as a clinician involved in Elite athlete care for the past few decades.
BiographyGhislaine Robert renowned International sports physician will present the science behind PRP and Stem cell injections used in regenerative medicine. Combining her knowledge and lengthy experience using PRP (Platelet Rich Plasma) and more recent-ly Stem Cells, Dr Robert will detail how these methods can be used to treat the whole patient naturally. She will go over indi-cations, contraindications and results with different procedures (both MSK and non MSK).
The Science behind PRP and Stem Cell injections used in regenerative medicineGhislaine Robert Sparclaine, USA
Ghislaine Robert, Biochem Mol biol J 2018 Volume: 4DOI: 10.21767/2471-8084-C2-010
Biotechnology 2018 | Stem Cell 2018 | Molecular Diagnostics 2018
E u r o S c i C o n J o i n t E v e n t o n
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S e s s i o n sMedical Biotechnology | Reproductive Biotechnology | Plant and Agriculture Biotechnology | Pharmaceutical Biotechnology | Clinical Research | Advancements In Biotechnology | Stem Cell Product Development & Commercialization | Stem Cell Transplantation & Techniques | Ethical Issues In Stem Cell Research | Tissue Regeneration & Therapeutics | Stem Cell apoptosis and signal transduction
Title: Efficacious dendritic cell based immunotherapy for advanced multiple malignanciesChandan R Bora, NOVO Cellular Medicine Institute - Fidelity Healthcare Ltd, Trinidad & Tobago
Title: Copyright in Gene SequencesMark Engelman, Head, Intellectual Property, UK
Title: Healing effect of Pistacia atlantica hydroalcoholic extract with bone marrow mesenchymal stem cells on knee OsteoarthritisOmid Koohi-Hosseinabadi, Shiraz University of Medical Science, Iran
Title: Viral Gene Delivery Tools In Optimization Of Human Mesenchymal Stem Cells’ Osteoblast DifferentiationOlexandr Korchynskyi, Rzeszow University, Poland
Title: Varicocele and its effects on the male reproductive systemZeliha Selamoglu, Nigde Omer Halisdemir University, Turkey
Title: Novel Trends in System Approach to Biotechnology-based Sustainable Development and BioeconomySlawomir Mikrut, Conosrtium Of The World University, Poland
Title: Imaging Cell Walls Of A Model Grass Brachypodium distachyonUtku AVCI, Recep Tayyip Erdogan University, Turkey
Title: 3D Neural Stem Cell Models of Alzheimer’s DiseaseMarina Wiklander, Merck, Sweden
Title: How To Place Innovation Priority In Medical Care Future In North Africa Abdessalam kaaniche, Tunisia Stem Cell Association, Tunisia
Title: Hairy root culture: a successful model for pharmaceutical compounds productionRaoufa Ahmed Abdel Rahman, City of Scientific Research & Technology Applications, Egypt
Session ChairMark Engelman, Head, Intellectual Property, UK
E u r o S c i C o n J o i n t E v e n t o n
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Biotechnology 2018 | Stem Cell 2018 | Molecular Diagnostics 2018Biochemistry & Molecular Biology JournalISSN 2471-8084
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Background & Aims: Dendritic cell (DC)-based immunotherapy is a promising viable tool in cancer treatment. Dendritic cell (DC)-based vaccination can provoke antitumor T cell responses in vivo. These case studies examined feasibility and outcome of DC-based tumor vaccination for patients with prostate cancer (n=5), breast cancer (n=2), cervical cancer (n=1), gastric cancer (n=1), pancreatic cancer (n=1) This approach has been used mostly in patients in the presence of defined tumor antigens.
Experimental Design: Accessible tumor tissue was disrupted into single cell suspensions. Autologous DCs were prepared from adherent peripheral blood mononuclear cells and cultured in granulocyte macrophage colony-stimulating factor, interleukin and autologous plasma. Tumor cells and DCs were co-cultured in the presence of polyethylene glycol to generate the fusions. Fusion cells were quantified by determining the percentage of cells that co-express tumor and DC markers. Patients vaccinated with three doses of DC (10×106) were administered after every 2 weeks intervals and assessed weekly for toxicity and tumor response was assessed at 3 months after completion of vaccination.
Results: Vaccination was well tolerated. No physical signs of autoimmunity were detected. None of the patients was found to meet the criteria complete responses. There was no evidence of significant toxicity from vaccine or adjuvant. There was increased expression of T helper type 1 cytokines. Vaccination resulted in a significant reduction in the level of prostate-specific antigen (PSA) in prostate cancer patient. There was 20% regression of tumor load in one prostate cancer patient. One patient with prostate cancer had surgery followed by Immunotherapy. PET scan reveals no further cancerous activity was found. Disease was stable upto 6 months in case of breast and cervical cancer patients.
Conclusion: DC-based vaccination can stimulate an antitumoral T cell response in patients with various cancers. These data indicate that vaccination with autologous tumor-pulsed DCs generated from peripheral blood is safe and can induce tumor-specific cellular cytotoxicity. Clinical responses are achievable, even in patients with advanced disease.
BiographyChandan R Bora completed his Master’s Degree from SGB Am-ravati University (India) and Doctorate Degree from Rani Durga-vati Vishwavidyalaya (India). He currently holds the position of a Director at NOVO Cellular Medicine Institute, Fidelity Healthcare Ltd, Trinidad & Tobago. His research areas are Immunology, Mi-crobiology and Molecular Biology. He has presented research papers in many national and international conferences. He has also published national and international research papers, re-views and book chapters. He is the Editorial Board Member of the journal BOAJ Cancer Research and Therapy and active Ad-visory Board Member of Bio Accent Group LLC. He has several years of experience in autologous cellular therapies especially dendritic cell therapy; activated T cell therapy and tumor infiltrat-ing lymphocyte therapy. He has actively participated in clinical trials on dendritic cells in solid tumors. He has experience in diagnostic industry especially related to immunocompromized diseases like AIDS, Cancer etc.
Efficacious dendritic cell-based immunotherapy for advanced multiple malignancies
Chandan R Bora1 and Pramod Dhembare21NOVO Cellular Medicine Institute - Fidelity Healthcare Ltd, Trinidad & Tobago 2NOVO Cellular Medicine Institute - Fidelity Healthcare Ltd, India
Chandan R Bora et al., Biochem Mol biol J 2018 Volume: 4DOI: 10.21767/2471-8084-C2-011
E u r o S c i C o n J o i n t E v e n t o n
Biotechnology, Stem Cell and
Molecular Diagnostics
Biotechnology 2018 | Stem Cell 2018 | Molecular Diagnostics 2018Biochemistry & Molecular Biology JournalISSN 2471-8084
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A discussion concerning the legal recognition of copyright rather than patent in DNA nucleotide sequences as they relate to Mechano growth
factor. A discussion of Emergent Genetics India Pvt. Ltd V Shailendra Shivam, Ravindra. With this case we finally have a ruling on the interesting debate on copyrightability of DNA sequences which had been brewing for some time.
Keywords— copyright, DNA Sequences, intellectual property
BiographyMark Engelman is a pharmacologist (King’s Lond.), trained as a barrister. Having practiced with in 2 firms of IP specialist solici-tors, 2 major multinationals, he is head of IP at Hardwicke, a set of barristers. He has been non-executive directors of compa-nies which include Alexander McQueen and the patent owner of the Mechano Growth Factor suit of patents. He acts for numer-ous multinationals. He is a Research Associate as St Edmunds College Cambridge in IP and Bencher of the Hon Soc. of Gray’s Inn, London. He is he legal correspondent for The Pharma Letter
Copyright in gene sequencesMark Engelman and Michael KockHead of Intellectual Property, Hardwicke Chambers, Research Associate in IP University of Cambridge, UK, Legal Correspondent The Pharma Letter, Director MRG & Associates LtdHead of Intellectual Property, Syngenta AG
Mark Engelman et al., Biochem Mol biol J 2018 Volume: 4DOI: 10.21767/2471-8084-C2-011
E u r o S c i C o n J o i n t E v e n t o n
Biotechnology, Stem Cell and
Molecular Diagnostics
Biotechnology 2018 | Stem Cell 2018 | Molecular Diagnostics 2018Biochemistry & Molecular Biology JournalISSN 2471-8084
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Introduction: Human skeleton is completely remodelled by mesenchymal stem cells (MSC) within roughly four-and-half years. Bone morphogenetic proteins (BMP) are main inducers of bone formation and maintenance apart of their crucial roles in morphogenesis of other tissues and organs. Optimal activity of BMPs in bone usually requires coordinate signals with the Wnt pathway. Consequently, several bone diseases occur when BMP or Wnt pathways are misregulated. Therefore, proper optimization of the osteogenesis requires efficient gene delivery tools on the way of modulation of osteoblast differentiation of MSC. Unfortunately, classical transfection with multiple transfection reagents triggers massive cell death in transduced MSC. On the other hand, viral tools for gene delivery allow to get 100% of efficiently transduced stem cells without any detection of toxicity.
Methodology: Human MSC cell were isolated from bone marrow aspirates obtained under patients’ consent from surgical material upon hip joint arthroplasty or from patients’ fat using a liposuction. Adv5-based vectors were used for efficient overexpression of recombinant genes into the mesenchymal precursor cells and lentiviral vectors were used for small hairpin RNA (shRNA)-mediated knockdown of gene expression.
Results & Discussions: The set of adenoviral and lentiviral vectors generated at our lab allows to overexpress or correspondingly downregulate expression of different genes important in regulation of osteogenesis. Usage of the gene delivery tools generated at our lab allowed us to describe a molecular mechanism that mediates osteogenesis regulation by secreted phosphoprotein 2.
Conclusions: Modern viral gene delivery tools open a wide window for biomedical studies aiming to investigate the molecular mechanisms of human MSC differentiation and to optimize the efficacy of regenerative skeletal tissues engineering based on MSC incorporation into the grafts.
BiographyO Korchynskyi has completed his PhD from Institute of Animal Biology, Lviv, Ukraine and Postdoctoral studies at the Nether-lands Cancer Institute, Amsterdam, Netherlands and Universi-ty of North Carolina, Chapel Hill, NC, USA. Currently he is the Associate Professor at the Rzeszow University, Poland and the Senior Scientist at the Institute of Cell Biology, Lviv, Ukraine. He has published more than 30 papers in reputed journals includ-ing Nature and Science.
Viral gene delivery tools in optimization of human mesenchymal stem cells’ osteoblast differentiation
O KorchynskyiMedical Faculty, Rzeszow University, Rzeszow, Poland and Institute of Cell Biology Natl. Acad.Sci, Lviv, Ukraine
O Korchynskyi, Biochem Mol biol J 2018 Volume: 4DOI: 10.21767/2471-8084-C2-011
E u r o S c i C o n J o i n t E v e n t o n
Biotechnology, Stem Cell and
Molecular Diagnostics
Biotechnology 2018 | Stem Cell 2018 | Molecular Diagnostics 2018Biochemistry & Molecular Biology JournalISSN 2471-8084
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Varicocele is a disorder characterized by abnormal dilation of testicular venules in the pampiniform plexus. This disorder is one of the most
important causes of infertility. This illness affects 15-22% of the adult male population and it is seen in 30-40% of the infertile patients. In cases of secondary infertility diagnosis, the incidence of varicoceles increases to 69-81%, while 80% of varicocele-diagnosed cases have no infertility. In many studies, varicoceles have been shown to cause progressive histopathologic alterationes in animal and human testes. In a project of the World Health Organization that shows Varicocel's effects on fertility; it is reported that this disease is associated with semen anomalies (deterioration in number and motility), testicular volume and decreased Leydig cell function. Although the varicocele pathophysiology has not yet been fully elucidated, it is asserted that it may caused from factors including oxidative stress, apoptosis, hormonal dysfunction, acrosome reaction defect, heavy metals, autoimmunity, hyperthermia, changes in testicular blood flow and venous pressure. The role of reactive oxygen species (ROS) in the development of varicocele and varicosele associated testicular damage has been investigated in many studies. Although an increase in ROS concentration was found in 80% of varicocele infertile patients, this increase was found in 77% of varicocele fertile man and in 20% of non-varicocele fertile individuals. In addition, the total antioxidant capacity of normal individuals was significantly higher than those of varicoceles. it was suggested that in patients with varicocele, apoptosis plays an important role in the development of oligospermia. In the one study suggests that during normal spermatogenesis 75% of all preleptotene spermatocytes can be removed by apoptosis. Although the most radical treatment of varicocele is surgical operations, recently has been shown some pharmacological agents (especially antioxidants) that can use as alternative treatment in cases of infertility due to varicoceles.
Keywords: Varicocele, antioxidants, testes, infertility, oxidative stress.
BiographyZeliha Selamoglu is a Professor in Medical Biology department of Nigde Omer Halisdemir University, Turkey. She earned her PhD in Biology from Inonu University, She has published over 90 peerreviewed journal articles with over 800 citations and many technical reports. She is a member of Society for Experimental Biology and Medicine: Associate Membership and European as-sociation for cancer research. She has served as Editorial Board member for many Journals.
Varicocele and its effects on the male reproductive systemFatih Mehmet Gur1, Zeliha Selamoglu2
1Department of Hstology&Embryology, Faculty of Medicine, Nigde Ömer Halisdemir University, Nigde, Turkey 2Department of Medical Biology, Faculty of Medicine, Nigde Ömer Halisdemir University, Nigde, Turkey
Zeliha Selamoglu et al., Biochem Mol biol J 2018 Volume: 4DOI: 10.21767/2471-8084-C2-011
E u r o S c i C o n J o i n t E v e n t o n
Biotechnology, Stem Cell and
Molecular Diagnostics
Biotechnology 2018 | Stem Cell 2018 | Molecular Diagnostics 2018Biochemistry & Molecular Biology JournalISSN 2471-8084
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Long-term transdisciplinary research and problem-solving training support integration of modern environmental and medical biotechnology, human
ecology, ecotoxicology, ecoengineering, remote sensing, architecture etc. for optimization sustainable management of the natural resources and promotion environmental health and bioeconomy-driven green labour market all over the world. Recommendation of system approach to Common Action of Experts and Knowledge-based Sustainable Society (including e-distance education and Life Long Learning) for Biotechnology driven better Quality of Life. Holistic approach should integrate wide scale use of modern biotechnology for as early as possible detection of synergistic effects of physical, chemical and biological risk factors ( in the natural and indoor environment and human food chain) with more efficient primary prevention. For prevention of incurable congenital malformations of disease of civilization Minamata as well as increasing rate of cancer/leukemia incidence are recommended biotechnologies introduced by the first of authors like evaluation of the synergistic effects of anthropogenic factors on embryological development, mutagenesis and carcinogenesis, including cell monitoring, free-radical pathology- oriented delay luminescence and other biophysical tools in linkage with monitoring of personal exposition and sensitivity, supplemented by nutritional and environmental health focused on better prevention of premature death due to environmental contamination. Introduced by JWD laser biotechnology is recommended for more efficient biodegradation of cancerogenic oil pollutants PAH, bioremediation toxic metals, reclamation of contaminated and semidesert areas, enhancement of biomass production by energy plantations, greeining cities, and for better adaptation of biotreatment and photodisinfection of wastewater and food production to climate change. For optimization selection areas predisposed for laser biotechnology introduction useful is application new methods of remote sensing e.g. for detection cultivated or forest areas of high phytotoxic effects followed by decrease of CO2 assimilation and biomass production by multispectral analyse e.g. fraction of Absorbed Photosynthetically
BiographyPh.D. of Jagiellonian University at age of 27 years, Sc.D. of W-M University in 1982, Professor in 2003 , Distinguish Professor in 2003. In 1968, initiated common action of university youth and Life Long Learning, followed by AGH Open University, National, International Schools, Workshops for Sustainable Develop-ment , Environmental Health Biodiversity and Bio-economy in the model regions from Poland, Brazil , China during 50 years. Chairman of 15 International Conferences on Sustainable De-velopment. Distinguish professor Jan W. DOBROWOLSKI, Ph.D., D.Sc., Dr.h.c. chairman of the International Team of Sustainable Development and Eco innovation (as well as 15 International Conferences in this field from 1989 to 2014),fellow of the World Academy of Arts and Science, Royal Academy of Science-Insti-tute of Spain, Consortium of the World University, Academeie Internationale des Sciences Ararat, Board of Directors of the International University of Bio-Environment and International Ansted University, professor of environmental engineering, bio-technology and human ecology of International University of Fundamental Studies, Section of Environmental Biotechnology EBS, Interuniversity Consortium of Biotechnology in Krakow, European Institute of Ecology and Cancer, founding member of the International Union of Elementologists and Balkan Acade-my of Sciences and Sustainable Development, etc.
Novel Trends in System Approach to Biotechnology-based Sustainable Development and BioeconomyJ.W.Dobrowolski1, E.Glowienka2, M. Mikrut2 and J,Koby-larczyk3 1World Academy of Arts and Science2AGH University of Science and Technology. Krakow. Poland3PK Cracow University of Technology, Krakow, Poland
J.W.Dobrowolski et al., Biochem Mol biol J 2018 Volume: 4DOI: 10.21767/2471-8084-C2-011
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Biotechnology, Stem Cell and
Molecular Diagnostics
Biotechnology 2018 | Stem Cell 2018 | Molecular Diagnostics 2018Biochemistry & Molecular Biology JournalISSN 2471-8084
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False brome, Brachypodium distachyon, has become a model system for grasses and cereals that are economically important. Plant cell walls are
currently growing research area in plant biology due to increasing interest in using plant biomass as feedstock for the production of biofuels and grasses are considered as a highly attractive feedstock. Cell walls are composed of polymers with complex and dynamic structures that can vary in cell types and different plant species, which makes better understanding of plant cell wall structure and function important. In order to address this, it is important to have powerful tools that can help imaging diverse glycans at the cellular level. Antibodies that recognize specific cell wall components are currently one of the most effective ways to determine the location and distribution of glycans in plant cell walls. The use of these cell Wall-direceted antibodies and immunolocalization of cell wall glycans in Brachypodium distachyon will be highlighted.
BiographyUtku AVCI is an assistant professor in the Bioengineering De-partment at Recep Tayyip Erdogan University in TURKEY. He obtained his PhD degree at the North Carolina State University in the USA. Then, he continued his academic research at the University of Georgia in the USA. For the last two years, he has been continuing his research in his country. His research is mainly on plant cell walls and lignocellulosic biofuel production.
Imaging Cell Walls Of A Model Grass Brachypodium distachyonUtku AVCIRecep Tayyip Erdogan University, Turkey
Utku AVCI, Biochem Mol biol J 2018 Volume: 4DOI: 10.21767/2471-8084-C2-011
E u r o S c i C o n J o i n t E v e n t o n
Biotechnology, Stem Cell and
Molecular Diagnostics
Biotechnology 2018 | Stem Cell 2018 | Molecular Diagnostics 2018Biochemistry & Molecular Biology JournalISSN 2471-8084
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Number of death caused by dementia has doubled in the last 15 years bringing it to the 7th leading cause of global death. Alzheimer’s is the main
form of dementia Characterized by amyloid-β plagues and neurofibrillary tangles. It is critical to establish a relevant disease model in order to understand the pathology of the dementia and also develop a drug.
Introduction of 3D cell culture technologies provided us with improved model for cellular research for drug discovery and other medical applications. It was very challenging to create reliable human disease model due to high levels of soluble and insoluble toxic amyloid β (Aβ) species that do not recapitulate the true Alzheimer’s disease pathology.
Recently, Kim et. al created a three-dimensional (3D) human neural stem cell model of Alzheimer’s disease using β-amyloid precursor protein and presenilin-1 overexpressing ReNcell™ VM human neural stem cell lines. This 3D cell model was able to induce robust extracellular deposition of amyloid-β, including amyloid-β plaques, and high levels of phosphorylated tau in the soma and neurites, as well as filamentous tau. This model is a valuable tool to study age-related Alzheimer’s dementia as well as other neurodegenerative disorders.
BiographyMarina Wiklander has completed her PhD in 2008 in Experimen-tal Haematology at the Uppsala University in Sweden at the de-partment of Genetics and Pathology. She is the Field Marketing Specialist for Cell Culture portfolio at Merck.
3D neural stem cell models of Alzheimer’s diseaseMarina WiklanderMerck, Sweden
Marina Wiklander, Biochem Mol biol J 2018 Volume: 4DOI: 10.21767/2471-8084-C2-011
E u r o S c i C o n J o i n t E v e n t o n
Biotechnology, Stem Cell and
Molecular Diagnostics
Biotechnology 2018 | Stem Cell 2018 | Molecular Diagnostics 2018Biochemistry & Molecular Biology JournalISSN 2471-8084
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Regenerative medicine represents a growing disruptive innovation that leads the actual quaternary care innovation; behind Information Technology (IT)
sector, both represent a future of an employment and great booming economic for emerging country. North Africa (NA), looks to lead continent with a growing economy based on education and medical services to safeguard the actual increasing rate of life quality for population that will exceed 200 million capital (NA) with 28% aged between 15 and 25 with net enrolment ratios (NER) average 94 percent. Growing infrastructure in Public health care and Universities during the last decade shows massive involvement of graduate in academic research with a majority oriented for medical care, biological science and its research subjects. Faced to the lack of the economic model, this begun to be as a shortness Politics solution against the high rate of unemployment. We promote through the emerging civil society activity the advocate for the development of the next economic model, on the horizon of 2030, to including bioscience like a head model for high added value industry and high skills demand market.
BiographyAbdessalam.J Kaaniche has involved his research studies in degenerative mechanism related the hematology and cardio-vascular, graduate from University of Paris Diderot. Since 2010 he is looking to open the opportunity, to be treated with regener-ative medicine, for the North African population.
He takes his responsibility in the position of the president, founder of the Tunisian Stem Cell Association as premier non-profit organization talks about this subject in the region.
He participates in event in regenerative medicine & stem cell research around the world and has been serving as a speaker to promote the North African region like a hub for regenerative medicine.
How to place innovation priority in medical care future in North Africa?Abdessalam J Kaaniche Tunisian Stem Cell Association, Tunisia
Abdessalam J Kaaniche, Biochem Mol biol J 2018 Volume: 4DOI: 10.21767/2471-8084-C2-011
E u r o S c i C o n J o i n t E v e n t o n
Biotechnology, Stem Cell and
Molecular Diagnostics
Biotechnology 2018 | Stem Cell 2018 | Molecular Diagnostics 2018Biochemistry & Molecular Biology JournalISSN 2471-8084
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Higher plants are still a major source of natural products also called secondary metabolites such as pharmaceuticals, colours, dyes, and
flavours. Several secondary metabolites from plants are used medicinally as isolated compounds, such as morphine (pain killer), paclitaxel, vinblastine (tumor therapy). Over the past three decades, plant tissue culture and plant biotechnology techniques have proved to be a valuable tool for study biosynthesis and production of pharmaceutical compounds in plants. One of the plant tissue culture protocols that considered as an attractive alternative for the production of many valuable natural secondary metabolites is hairy root culture. Hairy roots, also called transformed roots, are produced after infection of susceptible plant cells with a soil bacterium, Agrobacterium rhizogenes, and are associated with the integration of T-DNA from the bacterial Ri plasmid into chromosomal DNA in plant cells. Hairy roots are characterized by high growth rate and genetic stability. These genetically transformed roots can produce higher levels of secondary metabolites or amounts comparable to those of intact plants. Hairy root cultures offer promise for production of valuable secondary metabolites in many plants. In addition, the altered phenotype of hairy root is useful in plant breeding programs with plants of ornamental interest. The present talk gives an overview on the advantages and applications of hairy root culture, how to establish and culture them in labs, and examples of real experiments for using hairy root culture as a model for pharmaceutical compounds production from some Egyptian medicinal and ornamental plants.
BiographyRaoufa AbdelRahman is an associate professor of plant bio-technology and the head of Pharmaceutical Bio-Products Re-search Dep., Genetic Engineering & Biotechnology Research Institute, City of Scientific Research & Technology Applications, New Borg El-Arab, Alex., Egypt. She was a Ph.D. student at university of Georgia, Athens, USA. She completed a research project entitled "Exploring IRES mediated discistrons for the phytoremediation of Mercury". She examined several inter-nal ribosome entry sequences (IRES) to express the bacterial methylmercury lyase (merB) and mercuric ion reductase (merA) enzymes from single dicistronic transcripts in plants. Since 2003, she has been interested in using plant tissue culture techniques to conserve rare and endangered plant species, as well as enhance the productivity of important pharmaceutical compounds from plants. She is working in several projects deal-ing with the production of antiviral, anticancer, and antioxidant compounds from plants using Invitro culture. Also, she is using Agrobacterium mediated transformation methods to enhance pharmaceutical compounds productivity or establish transgen-ic plants resistant to specific environmental conditions.
Hairy root culture: a successful model for pharmaceutical compounds productionRaoufa Ahmed Abdel RahmanPharmaceutical Bio-Products Research Dep. Genetic Engineering & Biotechnology Research Institute. City of Scientific Research & Technology Applications, Egypt
Raoufa Ahmed Abdel Rahman, Biochem Mol biol J 2018 Volume: 4DOI: 10.21767/2471-8084-C2-011