Data Collection for David Gates

Cezanne & Taylor

B

T

T

T

T

d1

d1

d2

d2 d5m

d5m

d3

d3

d4

d4

2 nt clamp, comp to d5

David’s PNAS Catalytic Rxn (A+B->A+C)

DG1s

DG2-AC

d1

T

TT

d3

d4

A

Fuel-s

Rep.

C A

Courtesy of Matt Olson, 2010

A+B->A+CCourtesy of Matt Olson, 2010

1 2 3 4 5 6 X 7 8 9 # Contents

1 Ladder

2 DG2

3 DG2 + Fuel S

4 DG2 + DG1

5 DG1

6 DG1 + B

7 DG1 + A + B

8 Rep. Gate

9 Rep. Gate + DG2

A1B1

Backward B Out B

Helper BC

B1C2

Gate B(GB)

Translator Gate BC (TGBC)

Reporter Gate C (RGC)

Gates for A1 + B1 -> B1 + C2

1 2 3 4 5 6 7 8 9 # Contents

1 Ladder

2 TGBC

3 TGBC + Helper BC

4 GB

5 GB + A1

6 GB + B1

7 GB + A1 + B1

8 Rep. Gate C

9 TGBC + Rep. Gate C

A1B1

Backward B Out B

Helper B

B1B2

Gate B(GB)

Translator Gate B (TGB)

Reporter Gate B (RGB)

Gates for A1 + B1 -> B1 + B2

1 2 3 4 5 6 7 8 9 10 11 # Contents

1 Ladder

2 TGB

3 TGB + Helper B

4 TGB + GB

5 TGB + Rep. Gate B

6 Rep. Gate B

7 Rep Gate B + B1

8 Rep. Gate B + B2

9 Rep. bot B + B2

10 Rep. bot C irr + C2

11 Rep. bot B

A_1: CACAATACTATCATC AATCCCT CACTCTTCAAACCTC TAACCCTB_1: CATTCAAATCTCACC TTTCCCA CTAACAACTCTCTAC TAACCCAB_2: CCATTCATTCTCTCC TTTCCCA CTAACAACTCTCTAC TAACCCAC_2: CCTTCTTACACCTTC ATACCCT CTCATTCCTCACAAC ATTCCCA Backward B: CTAACAACTCTCTAC TAACCCA AATCCCT Out B: CACTCTTCAAACCTC TAACCCT CATTCAAATCTCACC TTT Gate_bot B: TG AGGGTTA GAGGTTTGAAGAGTG AGGGATT TGGGTTA GTAGAGAGTTGTTAG TGGGAAA Helper B: CCCA CT CCATTCATTCTCTCC TTTCCCA Trans_bot B: AG TGGGAAA GGAGAGAATGAATGG AG TGGGAAA GGTGAGATTTGAATG AGGGTTA Helper BC: CCCA CT CCTTCTTACACCTTC ATACCCT Trans_bot BC: AG AGGGTAT GAAGGTGTAAGAAGG AG TGGGAAA GGTGAGATTTGAATG AGGGTTA Rep_top B: CTAACAACTCTCTAC TAACCCA CCCAA /3IAbRQSp/Rep_bot B: /56-ROXN/ TTGGG TGGGTTA GTAGAGAGTTGTTAG TGGGAAA Rep_top C irr: CTCATTCCTCACAAC ATTCCCA /3IAbRQSp/Rep_bot C irr: /5TYE563/ TGGGAAT GTTGTGAGGAATGAG AGGGTAT