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Software PackagesMetaboAnalyst

XCMS

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MetaboAnalyst 2.0

• This is an online set of statistical tools

– http://www.metaboanalyst.ca/

• First, create an Excel file for each sample containing three columns, the m/z of each metabolite, its retention time and the peak intensity

• Put the files for a group in a folder.

• Then zip the two folders 

• On the MetaboAnalyst website, click on “Welcome, click here to start”

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Structure of Excel file for submission to MetaboAnalyst

MetaboAnalyst data entry

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Initial parameter setting

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Initial evaluation

More assessment of the data

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Data filtering

Data transformation and scaling

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Two‐fold change cutoff

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T‐test cutoff, p <0.05

Volcano plot

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Correlation matrix

Peaks differentiating Ctrl/OVEX

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PCA 2D‐plot

3D PCA plot

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PLSDA 2D‐plot

3D‐PLSDA plot

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Results from XCMS Online

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For in-depth statistical analysis and data interpretation, please make an appointment with a biostatistician.

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Thank you!