dantuluri narayana raju college(autonomous) (a … · 2 week 4 preservation of microbial cultures...

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DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS) (A College with Potential for Excellence) Bhimavaram, W.G.Dist, A.P Annual Curricular Plan for the Academic Year 2018-19 Department :U.G MICROBIOLOGY Paper 1A:INTRODUCTION TO MICROBIOLOGY&MICROBIAL DIVERSITY Class: I B.Sc MICROBIOLOGY Semester:I Month & No.of Working Days Week No.of Periods Per week Topics to be covered Co-Curricular Activity June - 22 1 Week 4 History and mile stones in microbiology. Contributions of Anton von Leeuwenhoek, 2 Week 4 Edward Jenner, Louis Pasteur, Robert Koch, Ivanowsky. Importance and applications of microbiology. Classification of microorganisms 3 Week 4 Haeckel’s three Kingdom concept, Whittaker’s five kingdom concept, three domain concept of Carl Woese. 4 Week 4 Outline classification of bacteria as per the second edition of Bergey’s Manual of Systematic Bacteriology. July - 25 1 Week 4 General characteristics of Bacteria, Archaea, Mycoplasmas and Cyanobacteria 2 Week 4 Ultra structure of Prokaryotic cell- Variant components and invariant components. General characteristics of viruses. 3 Week 4 Morphology, Structure and replication of TMV and HIV. 4 Week 4 General characteristics and outline classification of Fungi, Algae and Protozoa. Aug - 24 1 Week 4 Principles of microscopy - Bright field and Electron microscopy (SEM and TEM). 2 Week 4 Staining Techniques Simple and Differential (Gram Staining and Spore Staining). 3 Week 4 Sterilization and disinfection techniques - Physical methods autoclave, 4 Week 4 hot- air oven, pressure cooker, laminar air flow, filter sterilization,

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Page 1: DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS) (A … · 2 Week 4 Preservation of microbial cultures –subculturing, overlaying cultures with mineral oils ... 3 Principles of food preservation

DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS)

(A College with Potential for Excellence)

Bhimavaram, W.G.Dist, A.P

Annual Curricular Plan for the Academic Year 2018-19

Department :U.G MICROBIOLOGY Paper 1A:INTRODUCTION TO MICROBIOLOGY&MICROBIAL DIVERSITY Class: I B.Sc

MICROBIOLOGY Semester:I

Month & No.of

Working Days Week

No.of Periods

Per week Topics to be covered Co-Curricular Activity

June - 22

1 Week 4 History and mile stones in microbiology.

Contributions of Anton von Leeuwenhoek,

2 Week 4 Edward Jenner, Louis Pasteur, Robert Koch, Ivanowsky.

Importance and applications of microbiology.

Classification of microorganisms

3 Week 4

Haeckel’s three Kingdom concept, Whittaker’s five kingdom concept, three domain

concept of Carl Woese.

4 Week 4 Outline classification of bacteria as per the second edition of Bergey’s Manual of

Systematic Bacteriology.

July - 25

1 Week 4 General characteristics of Bacteria, Archaea, Mycoplasmas and Cyanobacteria

2 Week 4 Ultra structure of Prokaryotic cell- Variant components and invariant components.

General characteristics of viruses.

3 Week 4 Morphology, Structure and replication of TMV and HIV.

4 Week 4 General characteristics and outline classification of Fungi, Algae and Protozoa.

Aug - 24

1 Week 4 Principles of microscopy - Bright field and Electron microscopy (SEM and TEM).

2 Week 4 Staining Techniques –Simple and Differential (Gram Staining and Spore Staining).

3 Week 4 Sterilization and disinfection techniques - Physical methods – autoclave,

4 Week 4 hot- air oven, pressure cooker, laminar air flow, filter sterilization,

Page 2: DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS) (A … · 2 Week 4 Preservation of microbial cultures –subculturing, overlaying cultures with mineral oils ... 3 Principles of food preservation

Sept - 21

1 Week 4 Radiation methods – UV rays, Gamma rays.

2 Week 4 Chemical methods – alcohols, aldehydes,

3 Week 4 fumigants, phenols, halogens and hypochlorites.

4 Week 4 Isolation of Microorganisms from natural habitats.

Pure culture techniques

Oct- 19

1 Week 4

dilution-plating, Streak-plate, Spread-plate, Pour-Plate and micromanipulator.

Enrichment culturing.

2 Week 4 Preservation of microbial cultures – subculturing, overlaying cultures with mineral oils

3 Week 4 lyophilization, sand cultures, storage at low temperature.

Semester end Examinations

4 Week

Nov- 09 1 Week

Semester-end Examinations

2 Week

Signature of the Lecturer Signature of the HOD Signature of the Principal

Page 3: DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS) (A … · 2 Week 4 Preservation of microbial cultures –subculturing, overlaying cultures with mineral oils ... 3 Principles of food preservation

DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS)

(A College with Potential for Excellence)

Bhimavaram, W.G.Dist, A.P

Annual Curricular Plan for the Academic Year 2018-19

Department :U.G.MICROBIOLOGY Paper : 2A MICROBIAL GENETICS&MOLECULAR BIOLOGY Class: II B.Sc MICROBIOLOGY

Semester:III

Month & No.of

Working Days Week

No.of Periods

Per week Topics to be covered Co-Curricular Activity

June – 22

1 Week 4 DNA and RNA as genetic material.

Structure and organization of prokaryotic DNA.

2 Week 4

Extrachromosomal genetic elements – Plasmids and transposons in bacteria.

3 Week 4

Mutations – spontaneous and induced, base pair changes, frame shifts, deletions,

4 Week

4 inversions, tandem duplications, insertions.

July – 25

1 Week 4 Mutagens - Physical and Chemical mutagens.

2 Week 4 Outlines of DNA damage and repair mechanisms

3 Week 4 Genetic recombination in bacteria – Conjugation, Transformation and Transduction.

4 Week 4 Types of RNA and their functions.

Aug – 24

1 Week 4 Genetic code.

Structure of ribosomes.

2 Week 4 Types of genes – structural, constitutive, regulatory

3 Week 4 Protein synthesis – Transcription and translation.

4 Week 4 Regulation of gene expression in bacteria

Page 4: DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS) (A … · 2 Week 4 Preservation of microbial cultures –subculturing, overlaying cultures with mineral oils ... 3 Principles of food preservation

Sept – 21

1 Week 4 lac operon.

2 Week 4 Basic principles of genetic engineering.

3 Week 4 Restriction endonucleases, DNA polymerases and ligases.

4 Week 4 Vectors like pBR 322, M13.

Oct- 19

1 Week 4 Outlines of gene cloning methods.

2 Week 4 Polymerase chain reaction. Genomic and cDNA libraries.

3 Week 4

General account on application of genetic engineering

Semester-end Examinations

4 Week

Nov- 09 1 Week

Semester-end Examinations

2 Week

Signature of the Lecturer Signature of the HOD Signature of the Principal

Page 5: DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS) (A … · 2 Week 4 Preservation of microbial cultures –subculturing, overlaying cultures with mineral oils ... 3 Principles of food preservation

DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS)

(A College with Potential for Excellence)

Bhimavaram, W.G.Dist, A.P

Annual Curricular Plan for the Academic Year 2018-19

Department : U.G.MICROBIOLOGY Paper : 3A ENVIRONMENTAL&AGRICULTURAL MICROBIOLOGY Class: III B.Sc

MICROBIOLOGY Semester:V

Month & No.of

Working Days Week

No.of Periods

Per week Topics to be covered Co-Curricular Activity

June – 22

1 Week 3 Terrestrial Environment: Soil profile and soil microflora

2 Week 3 Aquatic Environment: Microflora of fresh water and marine habitats

3 Week 3 Atmosphere: Aeromicroflora and dispersal of microbes

4 Week 3

Role of microorganisms in nutrient cycling (Carbon, nitrogen, phosphorus)

July – 25

1 Week 3 Treatment and safety of drinking (potable) water, methods to detect potability of water

samples:

2 Week 3 standard qualitative procedure: presumptive test/MPN test, confirmed and completed

tests for faecal coliforms

3 Week 3 Membrane filter technique.Microbial interactions – mutualism,

4 Week 3 commensalism, antagonism, competition, parasitism, predation.

Aug – 24

1 Week 3 Outlines of Solid Waste management: Sources and types of solid waste, Methods of

solid waste disposal

2 Week 3 composting and sanitary landfill

3 Week 3 Liquid waste management: Composition and strength of sewage (BOD and COD)

4 Week 3 Primary, secondary (oxidation ponds, trickling filter, activated sludge process

Page 6: DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS) (A … · 2 Week 4 Preservation of microbial cultures –subculturing, overlaying cultures with mineral oils ... 3 Principles of food preservation

Sept – 21

1 Week 3 septic tank and tertiary sewage treatment.

2 Week 3 Plant Growth Promoting Microorganisms - Mycorrhizae, Rhizobia,

3 Week 3 Frankia, phosphate-solubilizers and Cyanobacteria

4 Week 3 Outlines of biological nitrogen fixation (symbiotic, non-symbiotic).

Biofertilizers - Rhizobium.

Oct- 19

1 Week 3 Concept of disease in plants. Symptoms of plant diseases caused by fungi, bacteria,

and viruses.

2 Week 3 Plant diseases - groundnut rust, Citrus canker and tomato leaf curl

3 Week 3

Principles of plant disease control.

Semester-end Examinations

4 Week

Nov- 09 1 Week

Semester-end Examinations

2 Week

Signature of the Lecturer Signature of the HOD Signature of the Principal

Page 7: DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS) (A … · 2 Week 4 Preservation of microbial cultures –subculturing, overlaying cultures with mineral oils ... 3 Principles of food preservation

DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS)

(A College with Potential for Excellence)

Bhimavaram, W.G.Dist, A.P

Annual Curricular Plan for the Academic Year 2018-19

Department : U.G.MICROBIOLOGY Paper :4AFOOD&INDUSTRIAL MICROBIOLOGY Class: III B,Sc MICROBIOLOGY

Semester:V

Month & No.of

Working Days Week

No.of Periods

Per week Topics to be covered Co-Curricular Activity

June - 22

1 Week 3 Intrinsic and extrinsic parameters that affect microbial growth in food

2 Week 3

Microbial spoilage of food - fruits, vegetables, milk, meat,

3 Week 3

egg, bread and canned foods

4 Week 3 Food intoxication (botulism).

Food-borne diseases (salmonellosis) and their detection.

July - 25

1 Week 3 Principles of food preservation - Physical and chemical methods.

2 Week 3 Fermented Dairy foods – cheese and yogurt.

3 Week 3 Microorganisms as food – SCP, edible mushrooms

4 Week 3 white button, oyster and paddy straw

Aug - 24

1 Week 3 Probiotics and their benefits

2 Week 3 Microorganisms of industrial importance – yeasts,(Saccharomyces cerevisiae) moulds

3 Week 3 Aspergillus niger,Bacteria(E.coli), actinomycetes (Streptomyces griseus).

4 Week 3 Outlines of Isolation and Screening and strain improvement of industrially-important

microorganisms.

Page 8: DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS) (A … · 2 Week 4 Preservation of microbial cultures –subculturing, overlaying cultures with mineral oils ... 3 Principles of food preservation

Sept - 21

1 Week 3 Types of fermentation processes – solid state, liquid state, batch, fed-batch, continuous

2 Week

3 Basic concepts of Design of fermenter.

Ingredients of Fermentation media

3 Week 3 Downstream processing - filtration, centrifugation,

4 Week 3 cell disruption, solvent extraction.

Oct- 19

1 Week 3 Microbial production of Industrial products - Citric acid

2 Week 3 Ethanol, amylases, penicillin,

3 Week 3

glutamic acid and vitamin B12.

Semester-end Examinations

4 Week

Nov- 09 1 Week

Semester-end Examinations

2 Week

Signature of the Lecturer Signature of the HOD Signature of the Principal

Page 9: DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS) (A … · 2 Week 4 Preservation of microbial cultures –subculturing, overlaying cultures with mineral oils ... 3 Principles of food preservation

DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS)

(A College with Potential for Excellence)

Bhimavaram, W.G.Dist, A.P

Annual Curricular Plan for the Academic Year 2018-19

Department :U.G MICROBIOLOGY Paper :1B MICROBIAL CHEMISTRY & METABOLISM Class: I B.Sc

Semester:II

Month & No.of

Working Days Week

No.of Periods

Per week Topics to be covered Co-Curricular Activity

Nov – 13

3 Week 4 Outline classification and general characteristics of carbohydrates (monosaccharides,

disaccharides and polysaccharides).

4 Week 4 General characteristics of amino acids and proteins.

Structure of nitrogenous bases, nucleotides, nucleic acids

Dec – 23

1 Week 4 Fatty acids (saturated and unsaturated)

lipids (sphingolipds, sterols and phospholipids).

2 Week

4 Principle and applications of -

Colorimerty

3 Week 4 Chromatography (paper, thin-layer and affinity chomatography )

4 Week 4 Spectrophotometry (UV & visible),

Centrifugation

Jan – 18

1 Week 4 Properties and classification of Enzymes.

Biocatalysis- induced fit and lock and key models

2 Week

4 Role of Coenzymes and Cofactors in enzyme activity.

Factors affecting catalytic activity of enzymes.

3 Week 4 Inhibition of enzyme activity- competitive, noncompetitive, uncompetitive and

allosteric.

4 Week

4 Microbial Nutrition –Nutritional requirements and uptake of nutrients by cells.

Nutritional groups of microcroorganisms- autotrophs, heterotrophs, mixotrophs.

Feb – 21 1 Week 4 Outlines of oxygenic and anoxygenic photosynthesis in bacteria

Growth media- synthetic, complex, selective, enrichment and differential media

Page 10: DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS) (A … · 2 Week 4 Preservation of microbial cultures –subculturing, overlaying cultures with mineral oils ... 3 Principles of food preservation

2 Week

4 Microbial Growth- different phases of growth in batch cultures, Synchronous,

continuous, biphasic growth.

3 Week 4 Factors influencing microbial growth.

Methods for measuring microbial growth – Direct microscopy, viable count estimates

4 Week 4 Aerobic respiration -Glycolysis, HMP path way, ED path way, TCA cycle, Electron

transport

Mar – 24

1 Week

4 oxidative and substrate level phosphorylation.

Anaerobic respiration (Nitrate).

2 Week 4 Fermentation - Alcohol and lactic acid fermentations.

3 Week Semester-end Examinations

4 Week

Signature of the Lecturer Signature of the HOD Signature of the Principal

Page 11: DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS) (A … · 2 Week 4 Preservation of microbial cultures –subculturing, overlaying cultures with mineral oils ... 3 Principles of food preservation

DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS)

(A College with Potential for Excellence)

Bhimavaram, W.G.Dist, A.P

Annual Curricular Plan for the Academic Year 2018-19

Department :U.G MICROBIOLOGY Paper :2B IMMUNOLOGY&MEDICAL MICROBIOLOGY

Class: II B.Sc Semester:IV

Month & No.of

Working Days Week

No.of Periods

Per week Topics to be covered Co-Curricular Activity

Nov – 13

3 Week 4 Types of immunity – innate and acquired; active and passive; humoral and cell-

mediated immunity.

4 Week

4 Primary and secondary organs of immune system – thymus, bursa fabricus, bone

marrow, spleen and lymph nodes.

Dec – 23

1 Week 4 Identiification and function of B and T lymphocytes, null cells, monocytes,

macrophages,

2 Week 4 neutrophils, basophils and eosinophils.

3 Week

4 Antigens – types, chemical nature, antigenic determinants, haptens.

Factors affecting antigenicity.

4 Week 4 Antibodies – basic structure, types, properties and functions of immunoglobulins

Jan – 18

1 Week

4 Types of antigen-antibody reactions - Agglutinations, Precipitation, Neutralization,

complement fixation, blood groups.

2 Week

4 Labeled antibody based techniques – ELISA, RIA and Immunofluroscence.

Monoclonal antibodies – production and applications.

3 Week 4 Concept of hypersensitivity and Autoimmunity.

4 Week

4 Normal flora of human body.

Host pathogen interactions: infection, invasion, pathogen, pathogenicity, virulence and

opportunistic infection,

Page 12: DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS) (A … · 2 Week 4 Preservation of microbial cultures –subculturing, overlaying cultures with mineral oils ... 3 Principles of food preservation

Feb – 21

1 Week

4 General principles of diagnostic microbiology- collection, transport and processing of

clinical samples.

2 Week 4 General methods of laboratory diagnosis - cultural, biochemical, serological and

molecular methods

3 Week 4 Antibacterial Agents- Penicillin, Streptomycin and Tetracycline.

Antifungal agents – Amphotericin B, Griseofulvin

4 Week 4 Antiviral substances - Amantadine and Acyclovir

Tests for antimicrobial susceptibility.

Mar – 24

1 Week

4 Brief account on antibiotic resistance in bacteria - Methicillin-resistant Staphylococcus

aureus (MRSA).

Vaccines – Natural and recombinant

2 Week

4 Bacterial diseases – Tuberculosis and Typhoid

Fungal diseases – Candidiasis.

Protozoal diseases – Malaria.

3 Week

Semester-end Examinations

4 Week

Signature of the Lecturer Signature of the HOD Signature of the Principal

Page 13: DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS) (A … · 2 Week 4 Preservation of microbial cultures –subculturing, overlaying cultures with mineral oils ... 3 Principles of food preservation

DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS)

(A College with Potential for Excellence)

Bhimavaram, W.G.Dist, A.P

Annual Curricular Plan for the Academic Year 2018-19

Department :U.G MICROBIOLOGY Paper :3B MICROBIAL BIOTECHNOLOGY Class: III B.Sc

Semester:VI

Month & No.of

Working Days Week

No.of Periods

Per week Topics to be covered Co-Curricular Activity

Nov – 13

3 Week

3 Microbial biotechnology: Scope and its applications in human therapeutics, agriculture

(Biofertilizers, PGPR, Mycorrhizae), environmental, and food technology.

4 Week 3 Genetically engineered microbes for industrial application: Bacteria and yeast

Dec – 23

1 Week

3 Recombinant microbial production processes in pharmaceutical industries -

Streptokinase, recombinant vaccines (Hepatitis B vaccine).

2 Week 3 Over view of production and applications of Microbial polysaccharides

3 Week 3 Bioplastics and Microbial biosensors

4 Week 3 Microbial based transformation of steroids and sterols.

Jan – 18

1 Week 3 Bio-catalytic processes and their industrial applications: Production of high fructose

syrup

2 Week 3 production of cocoa butter substitute.

3 Week 3 Immobilization methods and their application: Whole cell immobilization

4 Week

3 Bio-ethanol and bio-diesel production: commercial production from lignocellulosic

waste and algal biomass.

Page 14: DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS) (A … · 2 Week 4 Preservation of microbial cultures –subculturing, overlaying cultures with mineral oils ... 3 Principles of food preservation

Feb – 21

1 Week 3 Biogas production: Methane and hydrogen production using microbial culture.

2 Week 3 Microorganisms in bioremediation

3 Week 3 Degradation of xenobiotics.

4 Week 3 Mineral recovery, removal of heavy metals from aqueous effluents

Mar – 24

1 Week 3 Outlines of Intellectual Property Rights

2 Week 3 Patents, Copyrights, Trademarks

3 Week Semester-end Examinations

4 Week

Signature of the Lecturer Signature of the HOD Signature of the Principal

Page 15: DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS) (A … · 2 Week 4 Preservation of microbial cultures –subculturing, overlaying cultures with mineral oils ... 3 Principles of food preservation

DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS)

(A College with Potential for Excellence)

Bhimavaram, W.G.Dist, A.P

Annual Curricular Plan for the Academic Year 2017-18

Department :U.G MICROBIOLOGY Paper 1A:INTRODUCTION TO MICROBIOLOGY&MICROBIAL DIVERSITY Class: I B.Sc

MICROBIOLOGY Semester:I

Month & No.of

Working Days Week

No.of Periods

Per week Topics to be covered Co-Curricular Activity

June – 24

1 Week 4 History and mile stones in microbiology.

Contributions of Anton von Leeuwenhoek,

2 Week 4 Edward Jenner, Louis Pasteur, Robert Koch, Ivanowsky.

Importance and applications of microbiology.

Classification of microorganisms

3 Week 4

Haeckel’s three Kingdom concept, Whittaker’s five kingdom concept, three domain

concept of Carl Woese.

4 Week 4 Outline classification of bacteria as per the second edition of Bergey’s Manual of

Systematic Bacteriology.

July – 25

1 Week 4 General characteristics of Bacteria, Archaea, Mycoplasmas and Cyanobacteria

2 Week 4 Ultra structure of Prokaryotic cell- Variant components and invariant components.

General characteristics of viruses.

3 Week 4 Morphology, Structure and replication of TMV and HIV.

4 Week 4 General characteristics and outline classification of Fungi, Algae and Protozoa.

Aug – 21 1 Week 4

Principles of microscopy - Bright field and Electron microscopy (SEM and TEM).

2 Week 4 Staining Techniques –Simple and Differential (Gram Staining and Spore Staining).

Page 16: DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS) (A … · 2 Week 4 Preservation of microbial cultures –subculturing, overlaying cultures with mineral oils ... 3 Principles of food preservation

3 Week 4 Sterilization and disinfection techniques - Physical methods – autoclave,

4 Week 4 hot- air oven, pressure cooker, laminar air flow, filter sterilization,

Sept – 18

1 Week 4 Radiation methods – UV rays, Gamma rays.

2 Week 4 Chemical methods – alcohols, aldehydes,

3 Week 4 fumigants, phenols, halogens and hypochlorites.

4 Week 4 Isolation of Microorganisms from natural habitats.

Pure culture techiques

Oct- 22

1 Week 4

dilution-plating, Streak-plate, Spread-plate, Pour-Plate and micromanipulator.

Enrichment culturing.

2 Week 4 Preservation of microbial cultures – subculturing, overlaying cultures with mineral oils

3 Week 4 lyophilization, sand cultures, storage at low temperature.

Semester end Examinations

4 Week

Nov- 11 1 Week

Semester-end Examinations

2 Week

Signature of the Lecturer Signature of the HOD Signature of the Principal

Page 17: DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS) (A … · 2 Week 4 Preservation of microbial cultures –subculturing, overlaying cultures with mineral oils ... 3 Principles of food preservation

DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS)

(A College with Potential for Excellence)

Bhimavaram, W.G.Dist, A.P

Annual Curricular Plan for the Academic Year 2017-18

Department :U.G.MICROBIOLOGY Paper : 2A MICROBIAL GENETICS&MOLECULAR BIOLOGY Class: II B.Sc MICROBIOLOGY

Semester:III

Month & No.of

Working Days Week

No.of Periods

Per week Topics to be covered Co-Curricular Activity

June – 24

1 Week 4 DNA and RNA as genetic material.

Structure and organization of prokaryotic DNA.

2 Week 4 Extrachromosomal genetic elements – Plasmids and transposons in bacteria.

3 Week 4 Mutations – spontaneous and induced, base pair changes, frame shifts, deletions,

4 Week 4 inversions, tandem duplications, insertions.

July – 25

1 Week 4 Mutagens - Physical and Chemical mutagens.

2 Week 4 Outlines of DNA damage and repair mechanisms

3 Week 4 Genetic recombination in bacteria – Conjugation, Transformation and Transduction.

4 Week 4 Types of RNA and their functions.

Aug – 22

1 Week 4 Genetic code.

Structure of ribosomes.

2 Week 4 Types of genes – structural, constitutive, regulatory

3 Week 4 Protein synthesis – Transcription and translation.

4 Week 4 Regulation of gene expression in bacteria

Sept – 18

1 Week 4 lac operon.

2 Week 4 Basic principles of genetic engineering.

3 Week 4 Restriction endonucleases, DNA polymerases and ligases.

4 Week 4 Vectors like pBR 322, M13.

Page 18: DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS) (A … · 2 Week 4 Preservation of microbial cultures –subculturing, overlaying cultures with mineral oils ... 3 Principles of food preservation

Oct- 22

1 Week 4 Outlines of gene cloning methods.

2 Week 4 Polymerase chain reaction. Genomic and cDNA libraries.

3 Week 4 General account on application of genetic engineering

Semester-end Examinations

4 Week

Nov- 11 1 Week

Semester-end Examinations

2 Week

Signature of the Lecturer Signature of the HOD Signature of the Principal

Page 19: DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS) (A … · 2 Week 4 Preservation of microbial cultures –subculturing, overlaying cultures with mineral oils ... 3 Principles of food preservation

DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS)

(A College with Potential for Excellence)

Bhimavaram, W.G.Dist, A.P

Annual Curricular Plan for the Academic Year 2017-18

Department : U.G.MICROBIOLOGY Paper : 3A ENVIRONMENTAL&AGRICULTURAL MICROBIOLOGY Class: III B.Sc

MICROBIOLOGY Semester:V

Month & No.of

Working Days Week

No.of Periods

Per week Topics to be covered Co-Curricular Activity

June – 24

1 Week 3 Terrestrial Environment: Soil profile and soil microflora

2 Week 3 Aquatic Environment: Microflora of fresh water and marine habitats

3 Week 3 Atmosphere: Aeromicroflora and dispersal of microbes

4 Week 3 Role of microorganisms in nutrient cycling (Carbon, nitrogen, phosphorus)

July – 25

1 Week 3 Treatment and safety of drinking (potable) water, methods to detect potability of water

samples:

2 Week 3 standard qualitative procedure: presumptive test/MPN test, confirmed and completed

tests for faecal coliforms

3 Week 3 Membrane filter technique.Microbial interactions – mutualism,

4 Week 3 commensalism, antagonism, competition, parasitism, predation.

Aug – 21

1 Week 3 Outlines of Solid Waste management: Sources and types of solid waste, Methods of

solid waste disposal

2 Week 3 composting and sanitary landfill

3 Week 3 Liquid waste management: Composition and strength of sewage (BOD and COD)

4 Week 3 Primary, secondary (oxidation ponds, trickling filter, activated sludge process

Sept – 18 1 Week 3 septic tank and tertiary sewage treatment.

2 Week 3 Plant Growth Promoting Microorganisms - Mycorrhizae, Rhizobia,

Page 20: DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS) (A … · 2 Week 4 Preservation of microbial cultures –subculturing, overlaying cultures with mineral oils ... 3 Principles of food preservation

3 Week 3 Frankia, phosphate-solubilizers and Cyanobacteria

4 Week 3 Outlines of biological nitrogen fixation (symbiotic, non-symbiotic).

Biofertilizers - Rhizobium.

Oct- 22

1 Week 3 Concept of disease in plants. Symptoms of plant diseases caused by fungi, bacteria,

and viruses.

2 Week 3 Plant diseases - groundnut rust, Citrus canker and tomato leaf curl

3 Week 3 Principles of plant disease control.

Semester-end Examinations

4 Week

Nov- 11 1 Week

Semester-end Examinations

2 Week

Signature of the Lecturer Signature of the HOD Signature of the Principal

Page 21: DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS) (A … · 2 Week 4 Preservation of microbial cultures –subculturing, overlaying cultures with mineral oils ... 3 Principles of food preservation

DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS)

(A College with Potential for Excellence)

Bhimavaram, W.G.Dist, A.P

Annual Curricular Plan for the Academic Year 2017-18

Department : U.G.MICROBIOLOGY Paper : 4A FOOD&INDUSTRIAL MICROBIOLOGY Class: III B,Sc MICROBIOLOGY

Semester:V

Month & No.of

Working Days Week

No.of Periods

Per week Topics to be covered Co-Curricular Activity

June – 24

1 Week 3 Intrinsic and extrinsic parameters that affect microbial growth in food

2 Week 3 Microbial spoilage of food - fruits, vegetables, milk, meat,

3 Week 3 egg, bread and canned foods

4 Week 3 Food intoxication (botulism).

Food-borne diseases (salmonellosis) and their detection.

July – 25

1 Week 3 Principles of food preservation - Physical and chemical methods.

2 Week 3 Fermented Dairy foods – cheese and yogurt.

3 Week 3 Microorganisms as food – SCP, edible mushrooms

4 Week 3 white button, oyster and paddy straw

Aug – 21

1 Week 3 Probiotics and their benefits

2 Week 3 Microorganisms of industrial importance – yeasts,(Saccharomyces cerevisiae) moulds

3 Week 3 Aspergillus niger,Bacteria(E.coli), actinomycetes (Streptomyces griseus).

4 Week 3 Outlines of Isolation and Screening and strain improvement of industrially-important

microorganisms.

Sept –18

1 Week 3 Types of fermentation processes – solid state, liquid state, batch, fed-batch, continuous

2 Week 3 Basic concepts of Design of fermenter.

Ingredients of Fermentation media

Page 22: DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS) (A … · 2 Week 4 Preservation of microbial cultures –subculturing, overlaying cultures with mineral oils ... 3 Principles of food preservation

3 Week 3 Downstream processing - filtration, centrifugation,

4 Week 3 cell disruption, solvent extraction.

Oct- 22

1 Week 3 Microbial production of Industrial products - Citric acid

2 Week 3 Ethanol, amylases, penicillin,

3 Week 3 glutamic acid and vitamin B12.

Semester-end Examinations

4 Week

Nov- 11 1 Week

Semester-end Examinations

2 Week

Signature of the Lecturer Signature of the HOD Signature of the Principal

Page 23: DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS) (A … · 2 Week 4 Preservation of microbial cultures –subculturing, overlaying cultures with mineral oils ... 3 Principles of food preservation

DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS)

(A College with Potential for Excellence)

Bhimavaram, W.G.Dist, A.P

Annual Curricular Plan for the Academic Year 2017-18

Department :U.G MICROBIOLOGY Paper : 1B MICROBIAL CHEMISTRY & METABOLISM Class: I B.Sc

Semester:II

Month & No.of

Working Days Week

No.of Periods

Per week Topics to be covered Co-Curricular Activity

Nov – 14

3 Week 4 Outline classification and general characteristics of carbohydrates (monosaccharides,

disaccharides and polysaccharides).

4 Week 4 General characteristics of amino acids and proteins.

Structure of nitrogenous bases, nucleotides, nucleic acids

Dec – 23

1 Week 4 Fatty acids (saturated and unsaturated)

lipids (sphingolipds, sterols and phospholipids).

2 Week

4 Principle and applications of -

Colorimerty

3 Week 4 Chromatography (paper, thin-layer and affinity chomatography )

4 Week 4 Spectrophotometry (UV & visible),

Centrifugation

Jan – 17

1 Week 4 Properties and classification of Enzymes.

Biocatalysis- induced fit and lock and key models

2 Week

4 Role of Coenzymes and Cofactors in enzyme activity.

Factors affecting catalytic activity of enzymes.

3 Week 4 Inhibition of enzyme activity- competitive, noncompetitive, uncompetitive and

allosteric.

4 Week

4 Microbial Nutrition –Nutritional requirements and uptake of nutrients by cells.

Nutritional groups of microcroorganisms- autotrophs, heterotrophs, mixotrophs.

Feb – 22 1 Week 4 Outlines of oxygenic and anoxygenic photosynthesis in bacteria

Growth media- synthetic, complex, selective, enrichment and differential media

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2 Week

4 Microbial Growth- different phases of growth in batch cultures, Synchronous,

continuous, biphasic growth.

3 Week 4 Factors influencing microbial growth.

Methods for measuring microbial growth – Direct microscopy, viable count estimates

4 Week 4 Aerobic respiration -Glycolysis, HMP path way, ED path way, TCA cycle, Electron

transport

Mar – 23

1 Week

4 oxidative and substrate level phosphorylation.

Anaerobic respiration (Nitrate).

2 Week 4 Fermentation - Alcohol and lactic acid fermentations.

3 Week Semester-end Examinations

4 Week

Signature of the Lecturer Signature of the HOD Signature of the Principal

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DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS)

(A College with Potential for Excellence)

Bhimavaram, W.G.Dist, A.P

Annual Curricular Plan for the Academic Year 2017-18

Department :U.G MICROBIOLOGY Paper : 2B IMMUNOLOGY&MEDICAL MICROBIOLOGY

Class: II B.Sc Semester:IV

Month & No.of

Working Days Week

No.of Periods

Per week Topics to be covered Co-Curricular Activity

Nov – 14

3 Week 4 Types of immunity – innate and acquired; active and passive; humoral and cell-

mediated immunity.

4 Week

4 Primary and secondary organs of immune system – thymus, bursa fabricus, bone

marrow, spleen and lymph nodes.

Dec – 23

1 Week 4 Identiification and function of B and T lymphocytes, null cells, monocytes,

macrophages,

2 Week 4 neutrophils, basophils and eosinophils.

3 Week

4 Antigens – types, chemical nature, antigenic determinants, haptens.

Factors affecting antigenicity.

4 Week 4 Antibodies – basic structure, types, properties and functions of immunoglobulins

Jan – 17

1 Week

4 Types of antigen-antibody reactions - Agglutinations, Precipitation, Neutralization,

complement fixation, blood groups.

2 Week

4 Labeled antibody based techniques – ELISA, RIA and Immunofluroscence.

Monoclonal antibodies – production and applications.

3 Week 4 Concept of hypersensitivity and Autoimmunity.

4 Week

4 Normal flora of human body.

Host pathogen interactions: infection, invasion, pathogen, pathogenicity, virulence and

opportunistic infection,

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Feb – 22

1 Week

4 General principles of diagnostic microbiology- collection, transport and processing of

clinical samples.

2 Week 4 General methods of laboratory diagnosis - cultural, biochemical, serological and

molecular methods

3 Week 4 Antibacterial Agents- Penicillin, Streptomycin and Tetracycline.

Antifungal agents – Amphotericin B, Griseofulvin

4 Week 4 Antiviral substances - Amantadine and Acyclovir

Tests for antimicrobial susceptibility.

Mar – 23

1 Week

4 Brief account on antibiotic resistance in bacteria - Methicillin-resistant Staphylococcus

aureus (MRSA).

Vaccines – Natural and recombinant

2 Week

4 Bacterial diseases – Tuberculosis and Typhoid

Fungal diseases – Candidiasis.

Protozoal diseases – Malaria.

3 Week

Semester-end Examinations

4 Week

Signature of the Lecturer Signature of the HOD Signature of the Principal

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DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS)

(A College with Potential for Excellence)

Bhimavaram, W.G.Dist, A.P

Annual Curricular Plan for the Academic Year 2017-18

Department :U.G MICROBIOLOGY Paper : 3B MICROBIAL BIOTECHNOLOGY Class: III B.Sc

Semester:VI

Month & No.of

Working Days Week

No.of Periods

Per week Topics to be covered Co-Curricular Activity

Nov – 14

3 Week 3 Microbial biotechnology: Scope and its applications in human therapeutics, agriculture

(Biofertilizers, PGPR, Mycorrhizae), environmental, and food technology.

4 Week 3 Genetically engineered microbes for industrial application: Bacteria and yeast

Dec – 23

1 Week

3 Recombinant microbial production processes in pharmaceutical industries -

Streptokinase, recombinant vaccines (Hepatitis B vaccine).

2 Week 3 Over view of production and applications of Microbial polysaccharides

3 Week 3 Bioplastics and Microbial biosensors

4 Week 3 Microbial based transformation of steroids and sterols.

Jan – 17

1 Week 3 Bio-catalytic processes and their industrial applications: Production of high fructose

syrup

2 Week 3 production of cocoa butter substitute.

3 Week 3 Immobilization methods and their application: Whole cell immobilization

4 Week

3 Bio-ethanol and bio-diesel production: commercial production from lignocellulosic

waste and algal biomass.

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Feb – 22

1 Week 3 Biogas production: Methane and hydrogen production using microbial culture.

2 Week 3 Microorganisms in bioremediation

3 Week 3 Degradation of xenobiotics.

4 Week 3 Mineral recovery, removal of heavy metals from aqueous effluents

Mar – 23

1 Week 3 Outlines of Intellectual Property Rights

2 Week 3 Patents, Copyrights, Trademarks

3 Week Semester-end Examinations

4 Week

Signature of the Lecturer Signature of the HOD Signature of the Principal

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DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS)

(A College with Potential for Excellence)

Bhimavaram, W.G.Dist, A.P

Annual Curricular Plan for the Academic Year 2016-17

Department :U.G MICROBIOLOGY Paper : 1A INTRODUCTION TO MICROBIOLOGY&MICROBIAL DIVERSITY Class: I B.Sc

MICROBIOLOGY Semester:I

Month & No.of

Working Days Week

No.of Periods

Per week Topics to be covered Co-Curricular Activity

June – 26

1 Week 4 History and mile stones in microbiology.

Contributions of Anton von Leeuwenhoek,

2 Week 4 Edward Jenner, Louis Pasteur, Robert Koch, Ivanowsky.

Importance and applications of microbiology.

Classification of microorganisms

3 Week 4

Haeckel’s three Kingdom concept, Whittaker’s five kingdom concept, three domain

concept of Carl Woese.

4 Week 4 Outline classification of bacteria as per the second edition of Bergey’s Manual of

Systematic Bacteriology.

July – 24

1 Week 4 General characteristics of Bacteria, Archaea, Mycoplasmas and Cyanobacteria

2 Week 4 Ultra structure of Prokaryotic cell- Variant components and invariant components.

General characteristics of viruses.

3 Week 4 Morphology, Structure and replication of TMV and HIV.

4 Week 4 General characteristics and outline classification of Fungi, Algae and Protozoa.

Aug – 22

1 Week 4 Principles of microscopy - Bright field and Electron microscopy (SEM and TEM).

2 Week 4 Staining Techniques –Simple and Differential (Gram Staining and Spore Staining).

3 Week 4 Sterilization and disinfection techniques - Physical methods – autoclave,

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4 Week 4 hot- air oven, pressure cooker, laminar air flow, filter sterilization,

Sept – 23

1 Week 4 Radiation methods – UV rays, Gamma rays.

2 Week 4 Chemical methods – alcohols, aldehydes,

3 Week 4 fumigants, phenols, halogens and hypochlorites.

4 Week 4 Isolation of Microorganisms from natural habitats.

Pure culture techiques

Oct- 18

1 Week 4

dilution-plating, Streak-plate, Spread-plate, Pour-Plate and micromanipulator.

Enrichment culturing.

2 Week 4 Preservation of microbial cultures – subculturing, overlaying cultures with mineral oils

3 Week 4 lyophilization, sand cultures, storage at low temperature.

Semester end Examinations

4 Week

Nov- 11 1 Week

Semester-end Examinations

2 Week

Signature of the Lecturer Signature of the HOD Signature of the Principal

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DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS)

(A College with Potential for Excellence)

Bhimavaram, W.G.Dist, A.P

Annual Curricular Plan for the Academic Year 2016-17

Department :U.G.MICROBIOLOGY Paper : 2A MICROBIAL GENETICS&MOLECULAR BIOLOGY Class: II B.Sc MICROBIOLOGY

Semester:III

Month & No.of

Working Days Week

No.of Periods

Per week Topics to be covered Co-Curricular Activity

June – 26

1 Week 4 DNA and RNA as genetic material.

Structure and organization of prokaryotic DNA.

2 Week 4 Extrachromosomal genetic elements – Plasmids and transposons in bacteria.

3 Week 4 Mutations – spontaneous and induced, base pair changes, frame shifts, deletions,

4 Week 4 inversions, tandem duplications, insertions.

July – 24

1 Week 4 Mutagens - Physical and Chemical mutagens.

2 Week 4 Outlines of DNA damage and repair mechanisms

3 Week 4 Genetic recombination in bacteria – Conjugation, Transformation and Transduction.

4 Week 4 Types of RNA and their functions.

Aug – 22

1 Week 4 Genetic code.

Structure of ribosomes.

2 Week 4 Types of genes – structural, constitutive, regulatory

3 Week 4 Protein synthesis – Transcription and translation.

4 Week 4 Regulation of gene expression in bacteria

Sept – 23

1 Week 4 lac operon.

2 Week 4 Basic principles of genetic engineering.

3 Week 4 Restriction endonucleases, DNA polymerases and ligases.

4 Week 4 Vectors like pBR 322, M13.

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Oct- 18

1 Week 4 Outlines of gene cloning methods.

2 Week 4 Polymerase chain reaction. Genomic and cDNA libraries.

3 Week 4 General account on application of genetic engineering

Semester-end Examinations

4 Week

Nov- 11 1 Week

Semester-end Examinations

2 Week

Signature of the Lecturer Signature of the HOD Signature of the Principal

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DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS)

(A College with Potential for Excellence)

Bhimavaram, W.G.Dist, A.P

Annual Curricular Plan for the Academic Year 2016-17

Department :U.G.MICROBIOLOGY Paper : 3A IMMUNOLOGY Class: III B.Sc MICROBIOLOGY Semester:V

Month & No.of

Working Days Week

No.of Periods

Per week Topics to be covered Co-Curricular Activity

June – 26

1 Week 3 Types of immunity – innate and acquired; active and passive; humoral and cell-

mediated immunity

2 Week 3

Primary and secondary organs of immune system – thymus, bursa fabricus,

3 Week 3 bone marrow, spleen and lymph nodes.

4 Week 3

Identiification and function of B and T lymphocytes

July – 24

1 Week 3 null cells, monocytes, macrophages,

2 Week 3 neutrophils, basophils and eosinophils.

3 Week

3 Antigens – types, chemical nature, antigenic determinants, haptens.

Factors affecting antigenicity.

4 Week 3 Antibodies – basic structure, types,

Aug – 22

1 Week 3 properties and functions of immunoglobulins

2 Week 3 Types of antigen-antibody reactions - Agglutinations, Precipitation,

3 Week 3 Neutralization, complement fixation, blood groups.

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4 Week 3 Labeled antibody based techniques

Sept – 23

1 Week 3 ELISA, RIA

2 Week 3 Immunofluroscence.

3 Week 3 Polyclonal& monoclonal antibodies

4 Week 3 Monoclonal antibodies – production and applications.

Oct- 18

1 Week 3 Types of hypersensitivity-immediate&delayed

2 Week 3 Autoimmunity& its significance

3 Week Semester-end Examinations

4 Week

Nov- 11 1 Week

Semester-end Examinations

2 Week

Signature of the Lecturer Signature of the HOD Signature of the Principal

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DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS)

(A College with Potential for Excellence)

Bhimavaram, W.G.Dist, A.P

Annual Curricular Plan for the Academic Year 2016-17

Department :U.G.MICROBIOLOGY Paper : 4A ENVIRONMENTAL MICROBIOLOGY Class: III B.Sc MICROBIOLOGY Semester:V

Month & No.of

Working Days Week

No.of Periods

Per week Topics to be covered Co-Curricular Activity

June – 26

1 Week 3

Physical & chemical characteristics of soil

2 Week 3

Rhizosphere and phyllosphere

3 Week 3

Plant growth promoting microorganisms

4 Week 3

Outlines of biological nitrogen fixation(symbiotic& non symbiotic)

July – 24

1 Week 3 Biofertilizers&concept of diseases in plants

2 Week 3 Symptoms of plant diseases caused by fungi,bacteria

3 Week 3 Principles of plant disease control

4 Week 3 Biological control of plant diseases

Aug – 22

1 Week 3 Biopesticides-bt,NPV

2 Week 3 Microorganisms of environment

3 Week 3 Role of microorganisms in nutrient cycling

4 Week 3 Microbial interactions-Mutualism,commensalism,antagonism

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Sept – 23

1 Week 3 Microbiology of potable and polluted water

2 Week 3 Sanitation of potable water

3 Week 3 Sewage treatment(primary,secondary,tertiary)

4 Week 3 Outlines of biodegradation of environmental pollutants

Oct- 18

1 Week 3 Solid waste disposal ,sanitary land fills,composting

2 Week 3 Microbiology of air and air sampling methods

3 Week

Semester-end Examinations

4 Week

Nov- 11 1 Week

Semester-end Examinations

2 Week

Signature of the Lecturer Signature of the HOD Signature of the Principal

Page 37: DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS) (A … · 2 Week 4 Preservation of microbial cultures –subculturing, overlaying cultures with mineral oils ... 3 Principles of food preservation

DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS)

(A College with Potential for Excellence)

Bhimavaram, W.G.Dist, A.P

Annual Curricular Plan for the Academic Year 2016-17

Department :U.G MICROBIOLOGY Paper : 1B MICROBIAL CHEMISTRY & METABOLISM Class: I B.Sc

Semester:II

Month & No.of

Working Days Week

No.of Periods

Per week Topics to be covered Co-Curricular Activity

Nov – 14

3 Week 4 Outline classification and general characteristics of carbohydrates (monosaccharides,

disaccharides and polysaccharides).

4 Week 4 General characteristics of amino acids and proteins.

Structure of nitrogenous bases, nucleotides, nucleic acids

Dec – 24

1 Week 4 Fatty acids (saturated and unsaturated)

lipids (sphingolipds, sterols and phospholipids).

2 Week 4 Principle and applications of -

Colorimerty

3 Week 4 Chromatography (paper, thin-layer and affinity chomatography )

4 Week 4 Spectrophotometry (UV & visible),

Centrifugation

Jan – 19

1 Week 4 Properties and classification of Enzymes.

Biocatalysis- induced fit and lock and key models

2 Week

4 Role of Coenzymes and Cofactors in enzyme activity.

Factors affecting catalytic activity of enzymes.

3 Week 4 Inhibition of enzyme activity- competitive, noncompetitive, uncompetitive and

allosteric.

4 Week

4 Microbial Nutrition –Nutritional requirements and uptake of nutrients by cells.

Nutritional groups of microcroorganisms- autotrophs, heterotrophs, mixotrophs.

Feb – 20

1 Week 4 Outlines of oxygenic and anoxygenic photosynthesis in bacteria

Growth media- synthetic, complex, selective, enrichment and differential media

2 Week 4 Microbial Growth- different phases of growth in batch cultures, Synchronous,

continuous, biphasic growth.

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3 Week 4 Factors influencing microbial growth.

Methods for measuring microbial growth – Direct microscopy, viable count estimates

4 Week 4 Aerobic respiration -Glycolysis, HMP path way, ED path way, TCA cycle, Electron

transport

Mar – 25

1 Week

4 oxidative and substrate level phosphorylation.

Anaerobic respiration (Nitrate).

2 Week 4 Fermentation - Alcohol and lactic acid fermentations.

3 Week Semester-end Examinations

4 Week

Signature of the Lecturer Signature of the HOD Signature of the Principal

Page 39: DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS) (A … · 2 Week 4 Preservation of microbial cultures –subculturing, overlaying cultures with mineral oils ... 3 Principles of food preservation

DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS)

(A College with Potential for Excellence)

Bhimavaram, W.G.Dist, A.P

Annual Curricular Plan for the Academic Year 2016-17

Department :U.G MICROBIOLOGY Paper : 2B IMMUNOLOGY&MEDICAL MICROBIOLOGY

Class: II B.Sc Semester:IV

Month & No.of

Working Days Week

No.of Periods

Per week Topics to be covered Co-Curricular Activity

Nov – 14

3 Week 4 Types of immunity – innate and acquired; active and passive; humoral and cell-

mediated immunity.

4 Week 4 Primary and secondary organs of immune system – thymus, bursa fabricus, bone

marrow, spleen and lymph nodes.

Dec – 24

1 Week 4 Identiification and function of B and T lymphocytes, null cells, monocytes,

macrophages,

2 Week 4 neutrophils, basophils and eosinophils.

3 Week

4 Antigens – types, chemical nature, antigenic determinants, haptens.

Factors affecting antigenicity.

4 Week 4 Antibodies – basic structure, types, properties and functions of immunoglobulins

Jan – 19

1 Week

4 Types of antigen-antibody reactions - Agglutinations, Precipitation, Neutralization,

complement fixation, blood groups.

2 Week

4 Labeled antibody based techniques – ELISA, RIA and Immunofluroscence.

Monoclonal antibodies – production and applications.

3 Week 4 Concept of hypersensitivity and Autoimmunity.

4 Week

4 Normal flora of human body.

Host pathogen interactions: infection, invasion, pathogen, pathogenicity, virulence and

opportunistic infection,

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Feb – 20

1 Week

4 General principles of diagnostic microbiology- collection, transport and processing of

clinical samples.

2 Week 4 General methods of laboratory diagnosis - cultural, biochemical, serological and

molecular methods

3 Week 4 Antibacterial Agents- Penicillin, Streptomycin and Tetracycline.

Antifungal agents – Amphotericin B, Griseofulvin

4 Week 4 Antiviral substances - Amantadine and Acyclovir

Tests for antimicrobial susceptibility.

Mar – 25

1 Week

4 Brief account on antibiotic resistance in bacteria - Methicillin-resistant Staphylococcus

aureus (MRSA).

Vaccines – Natural and recombinant

2 Week

4 Bacterial diseases – Tuberculosis and Typhoid

Fungal diseases – Candidiasis.

Protozoal diseases – Malaria.

3 Week

Semester-end Examinations

4 Week

Signature of the Lecturer Signature of the HOD Signature of the Principal

Page 41: DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS) (A … · 2 Week 4 Preservation of microbial cultures –subculturing, overlaying cultures with mineral oils ... 3 Principles of food preservation

DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS)

(A College with Potential for Excellence)

Bhimavaram, W.G.Dist, A.P

Annual Curricular Plan for the Academic Year 2016-17

Department :U.G MICROBIOLOGY Paper 3B MEDICAL MICROBIOLOGY Class: IIIB.Sc

Semester:VI

Month & No.of

Working Days Week

No.of Periods

Per week Topics to be covered Co-Curricular Activity

Nov – 14

3 Week 3 History of Medical microbiology

4 Week 3 Normal flora of human body

Dec – 24

1 Week 3 Definition of infection,non-specific defence mechanism

2 Week 3 Mechanical barriers,antagonism of indigenous flora

3 Week 3 Antibacterial substances-lysozyme,complement

4 Week 3 Properdin,antiviral substances,phagocytosis

Jan – 19

1 Week 3 General principles of diagnostic microbiology

2 Week 3 collection, transport and processing of clinical samples.

3 Week 3 General methods of laboratory diagnosis - cultural, biochemical serological and

molecular methods

4 Week 3 Tests for antimicrobial susceptibility

Feb – 20

1 Week 3 Antiviral agents-interferons Host pathogen interactions.bacterial toxins

2 Week 3 Elements of chemotherapy,mode of action of penicillin

3 Week 3 Prevention and control of diseases& vaccines

4 Week 3 Airborne diseases-Tuberculosis,food borne diseases-cholera,typhoid

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Mar – 25

1 Week 3 Insect borne diseases-Malaria,dengue fever

2 Week 3 Contact and zoonotic diseases,blood borne diseases

3 Week

Semester-end Examinations

4 Week

Signature of the Lecturer Signature of the HOD Signature of the Principal

Page 43: DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS) (A … · 2 Week 4 Preservation of microbial cultures –subculturing, overlaying cultures with mineral oils ... 3 Principles of food preservation

DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS)

(A College with Potential for Excellence)

Bhimavaram, W.G.Dist, A.P

Annual Curricular Plan for the Academic Year 2016-17

Department :U.G MICROBIOLOGY Paper :4B FOOD&INDUSTRIAL MICROBIOLOGY Class: III B.Sc

Semester:VI

Month & No.of

Working Days Week

No.of Periods

Per week Topics to be covered Co-Curricular Activity

Nov – 14

3 Week 3 Microbiology of food spoilage and their sources

4 Week 3

Microbial spoilage of food - fruits, vegetables, milk, meat,

Dec – 24

1 Week 3

egg, bread and canned foods

2 Week 3 Food intoxication (botulism).

Food-borne diseases (salmonellosis) and their detection.

3 Week 3 Principles of food preservation - Physical and chemical methods.

4 Week 3 Fermented Dairy foods – cheese and yogurt.

Jan – 19

1 Week 3 Microorganisms as food – SCP, edible mushrooms

2 Week 3 white button, oyster and paddy straw

3 Week 3 Probiotics and their benefits

4 Week 3 Microorganisms of industrial importance – yeasts,(Saccharomyces cerevisiae) moulds

Feb – 20

1 Week 3 Aspergillus niger,Bacteria(E.coli), actinomycetes (Streptomyces griseus).

2 Week 3 Outlines of Isolation and Screening and strain improvement of industrially-important

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microorganisms.

3 Week 3 Types of fermentation processes – solid state, liquid state, batch, fed-batch, continuous

4 Week

3 Basic concepts of Design of fermenter.

Ingredients of Fermentation media

Mar – 25

1 Week 3 Microbial production of Industrial products - Citric acid

Ethanol, amylases, penicillin,

2 Week 3 glutamic acid and vitamin B12.

3 Week

Semester-end Examinations

4 Week

Signature of the Lecturer Signature of the HOD Signature of the Principal

Page 45: DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS) (A … · 2 Week 4 Preservation of microbial cultures –subculturing, overlaying cultures with mineral oils ... 3 Principles of food preservation

DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS)

(A College with Potential for Excellence)

Bhimavaram, W.G.Dist, A.P

Annual Curricular Plan for the Academic Year 2015-16

Department :U.G MICROBIOLOGY Paper : 1A Introduction to Microbiology, Microbial Techniques and Biology of Microorganisms. Class: I B.Sc Semester:I

Month & No.of

Working Days Week

No.of Periods

Per week Topics to be covered Co-Curricular Activity

June – 22

1 Week 4 History and mile stones in Microbiology-meaning, definition and history of

microbiology

2 Week 4

Contributions of antony van leeuwenhoek,Edward jenner,Louis pasteur

3 Week 4

Robert Koch ,Iwanowsky,Beijerinck,winogradsky

4 Week 4

Importance & applications of Microbiology

July - 25

1 Week 4 Taxonomy and significance of virology

2 Week 4 Protozoa(Entamoeba,leishmania)

3 Week 4 Isolation and identification of microorganisms

4 Week 4 Principles and types of stains

Aug - 22

1 Week 4 Simple ,Differential & negative stains

2 Week 4 Sterilization and disinfection techniques

3 Week 4 Physical methods of sterilization-autoclave,hot air oven,pressure cooker

4 Week 4 Radiation methods-U.V RAYS and ultrasonic methods

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Sept - 23

1 Week 4 Chemical methods –use of alcohols, aldehydes,fumigants,phenols

2 Week 4 Isolation of pure culture techniques-enrichment culturing, dilution plating

3 Week 4 Streak plate,spread plate & micromanipulator

4 Week 4 Preservation of microbial culture-subculturing,overlaying cultures with mineral oil

Oct- 19

1 Week 4 Lyophilization, sand cultures

2 Week 4 Archae-bacteria, Actinomycetes

3 Week 4

Structure and replication ofTMV

4 Week

Nov- 09 1 Week

Semester-end Examinations

2 Week

Signature of the Lecturer Signature of the HOD Signature of the Principal

Page 47: DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS) (A … · 2 Week 4 Preservation of microbial cultures –subculturing, overlaying cultures with mineral oils ... 3 Principles of food preservation

DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS)

(A College with Potential for Excellence)

Bhimavaram, W.G.Dist, A.P

Annual Curricular Plan for the Academic Year 2015-16

Department :U.G MICROBIOLOGY Paper : 2A Microbial Physiology and Genetics Class: II B.Sc

Semester:III

Month & No.of

Working Days Week

No.of Periods

Per week Topics to be covered Co-Curricular Activity

June - 22

1 Week 4 Nutritional requirements and uptake of nutrients by cells

2 Week 4

Nutritional groups of Microorganisms

3 Week 4

Autotrophs, heterotrophs, Mixotrophs,Methylotrophs

4 Week 4

Different phases of growth in batch culture

July - 25

1 Week 4 Factors influencing microbial growth

2 Week 4 Synchronous and continuous growth

3 Week 4 Biphasic growth, methods of measuring

4 Week 4 Microbial growth-direct microscopy

Aug - 22

1 Week 4 Viable count estimates,turbidometry,biomass

2 Week 4 Enzymes-properties and classification

3 Week 4 Enzyme unit ,bioatalysis-induced fit, lock and key model

4 Week 4 Coenzymes and cofactors

Sept - 23

1 Week 4 Factors affecting catalytic activity

2 Week 4 Inhibition of Enzyme activity-competitive,uncompetitive and

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allosteric

3 Week 4 Glycolysis,HMP Pathway,ED Pathway

4 Week 4 Electron transport,Oxidative and substrate level phosphorylation

Oct- 19

1 Week 4 Anaplerotic reactions,glyoxylate cycle

2 Week 4 Fermentaion and oxygenic and anoxygenic photosynthesis

3 Week Semester-end Examinations

4 Week

Nov- 09 1 Week

Semester-end Examinations

2 Week

Signature of the Lecturer Signature of the HOD Signature of the Principal

Page 49: DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS) (A … · 2 Week 4 Preservation of microbial cultures –subculturing, overlaying cultures with mineral oils ... 3 Principles of food preservation

DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS)

(A College with Potential for Excellence)

Bhimavaram, W.G.Dist, A.P

Annual Curricular Plan for the Academic Year 2015-16

Department :U.G MICROBIOLOGY Paper : 3A IMMUNOLOGY Class: III B.Sc

Semester:V

Month & No.of

Working Days Week

No.of Periods

Per week Topics to be covered Co-Curricular Activity

June - 22

1 Week 3 Types of immunity – innate and acquired; active and passive; humoral and cell-

mediated immunity

2 Week 3

Primary and secondary organs of immune system – thymus, bursa fabricus,

3 Week 3 bone marrow, spleen and lymph nodes.

4 Week 3

Identiification and function of B and T lymphocytes

July - 25

1 Week 3 null cells, monocytes, macrophages,

2 Week 3 neutrophils, basophils and eosinophils.

3 Week

3 Antigens – types, chemical nature, antigenic determinants, haptens.

Factors affecting antigenicity.

4 Week 3 Antibodies – basic structure, types,

Aug - 22

1 Week 3 properties and functions of immunoglobulins

2 Week 3 Types of antigen-antibody reactions - Agglutinations, Precipitation,

3 Week 3 Neutralization, complement fixation, blood groups.

4 Week 3 Labeled antibody based techniques

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Sept - 23

1 Week 3 ELISA, RIA

2 Week 3 Immunofluroscence.

3 Week 3 Polyclonal& monoclonal antibodies

4 Week 3 Monoclonal antibodies – production and applications.

Oct- 19

1 Week 3 Types of hypersensitivity-immediate&delayed

2 Week 3 Autoimmunity& its significance

3 Week Semester-end Examinations

4 Week

Nov- 09 1 Week

Semester-end Examinations

2 Week

Signature of the Lecturer Signature of the HOD Signature of the Principal

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DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS)

(A College with Potential for Excellence)

Bhimavaram, W.G.Dist, A.P

Annual Curricular Plan for the Academic Year 2015-16

Department :U.G MICROBIOLOGY Paper :4A ENVIRONMENTAL MICROBIOLOGY Class: III B.Sc

Semester:v

Month & No.of

Working Days Week

No.of Periods

Per week Topics to be covered Co-Curricular Activity

June - 22

1 Week 3 Physical & chemical characteristics of soil

2 Week 3

Rhizosphere and phyllosphere

3 Week 3

Plant growth promoting microorganisms

4 Week 3

Outlines of biological nitrogen fixation(symbiotic& non symbiotic)

July - 25

1 Week 3 Biofertilizers&concept of diseases in plants

2 Week 3 Symptoms of plant diseases caused by fungi,bacteria

3 Week 3 Principles of plant disease control

4 Week 3 Biological control of plant diseases

Aug - 22

1 Week 3 Biopesticides-bt,NPV

2 Week 3 Microorganisms of environment

3 Week 3 Role of microorganisms in nutrient cycling

4 Week 3 Microbial interactions-Mutualism,commensalism,antagonism

Sept - 23

1 Week 3 Microbiology of potable and polluted water

2 Week 3 Sanitation of potable water

Page 52: DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS) (A … · 2 Week 4 Preservation of microbial cultures –subculturing, overlaying cultures with mineral oils ... 3 Principles of food preservation

3 Week 3 Sewage treatment(primary,secondary,tertiary)

4 Week 3 Outlines of biodegradation of environmental pollutants

Oct- 19

1 Week 3 Solid waste disposal ,sanitary land fills,composting

2 Week 3 Microbiology of air and air sampling methods

3 Week Semester-end Examinations

4 Week

Nov- 09 1 Week

Semester-end Examinations

2 Week

Signature of the Lecturer Signature of the HOD Signature of the Principal

Page 53: DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS) (A … · 2 Week 4 Preservation of microbial cultures –subculturing, overlaying cultures with mineral oils ... 3 Principles of food preservation

DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS)

(A College with Potential for Excellence)

Bhimavaram, W.G.Dist, A.P

Annual Curricular Plan for the Academic Year 2015-16

Department :U.G MICROBIOLOGY Paper :IB Biology of prokaryotic and Eukaryotic Microorganisms

Class: I B.Sc Semester:II

Month & No.of

Working Days Week

No.of Periods

Per week Topics to be covered Co-Curricular Activity

Nov - 13

3 Week 4 Outline classification of living organisms,Haeckel ,whittaker carl woese systems

4 Week 4 Differentiation of prokaryotes and eukaryotes,general characteristics of bacteria

Dec - 24

1 Week 4 Archaebacteria,rickettsias ,mycoplasma,cynobacteria and actinomycetes

2 Week 4 Outline classification of bacteria as per the second edition of bergeys manual

3 Week 4 Ultra structure of bacterial cell, invariants and variants copmonents

4 Week 4 General characterisric and classification of viruses, morphology of TMV & HIV

Jan - 17

1 Week 4 General characterisric and classification of Eukaryotic microorganisms

2 Week 4 Outline classification and general characteristics of carbohydrates

3 Week 4 General characteristics of proteins

4 Week 4 Structure of nitrogenous bases,nucleotides,nucleic acids

Feb - 19

1 Week 4 Fatty acids (saturated and unsaturated)

2 Week 4 Lipids (spingolipids,sterols and phospholipids)

Page 54: DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS) (A … · 2 Week 4 Preservation of microbial cultures –subculturing, overlaying cultures with mineral oils ... 3 Principles of food preservation

3 Week 4 Hydrogen ion concentration in biological fluids

4 Week 4 Types of Buffers and their use in biological reactions

Mar - 23

1 Week 4 Principle and application of colorimeter

2 Week 4 Chromatography(paper and Thin layer)

3 Week Semester-end Examinations

4 Week

Signature of the Lecturer Signature of the HOD Signature of the Principal

Page 55: DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS) (A … · 2 Week 4 Preservation of microbial cultures –subculturing, overlaying cultures with mineral oils ... 3 Principles of food preservation

DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS)

(A College with Potential for Excellence)

Bhimavaram, W.G.Dist, A.P

Annual Curricular Plan for the Academic Year 2015-16

Department :U.G MICROBIOLOGY Paper : 2B Molecular Biology Class: II B.Sc

Semester:IV

Month & No.of

Working Days Week

No.of Periods

Per week Topics to be covered Co-Curricular Activity

Nov - 13

3 Week 4 DNA & RNA as genetic materials.structure of DNA

4 Week 4 Extra chromosomal genetic elements-plasmids and transposons

Dec - 24

1 Week 4 Replication of DNA,Proteins involved in DNA Replication

2 Week 4 Outlines of Damage and Repair mechanism

3 Week 4 Spontaneous and Induced base pair changes

4 Week 4 Frame shifts deletions,invertions,tandem duplications

Jan - 17

1 Week 4 Various physical and chemical mutagens

2 Week 4 Gene transfer mechanisms in bacteria-transformation,transduction and conjugation

3 Week 4 Concept of gene –muton,recon and cistron

4 Week 4 One gene-one enzyme,one gene- one polypeptide

Feb - 19

1 Week 4 Types of RNA and their functions

2 Week 4 Genetic code –structure of , ribosomes and protein synthesis,operon concept

3 Week 4 Regulation of gene expression in bacteria,lac operon

Page 56: DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS) (A … · 2 Week 4 Preservation of microbial cultures –subculturing, overlaying cultures with mineral oils ... 3 Principles of food preservation

4 Week 4 Basic principles of genetic engineering-restriction endonucleases

Mar - 23

1 Week 4 DNA polymerases,ligases,vectors,outlines of gene cloning methods

2 Week 4 General account on applications of genetic engineering.

3 Week Semester-end Examinations

4 Week

Signature of the Lecturer Signature of the HOD Signature of the Principal

Page 57: DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS) (A … · 2 Week 4 Preservation of microbial cultures –subculturing, overlaying cultures with mineral oils ... 3 Principles of food preservation

DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS)

(A College with Potential for Excellence)

Bhimavaram, W.G.Dist, A.P

Annual Curricular Plan for the Academic Year 2015-16

Department :U.G MICROBIOOGY Paper : 3B Medical Microbiology Class: III B.Sc

Semester:VI

Month & No.of

Working Days Week

No.of Periods

Per week Topics to be covered Co-Curricular Activity

Nov - 13

3 Week 3 History of Medical microbiology

4 Week 3 Normal flora of human body

Dec - 24

1 Week 3 Definition of infection,non-specific defence mechanism

2 Week 3 Mechanical barriers,antagonism of indigenous flora

3 Week 3 Antibacterial substances-lysozyme,complement

4 Week 3 Properdin,antiviral substances,phagocytosis

Jan - 17

1 Week 3 General principles of diagnostic microbiology

2 Week 3 collection, transport and processing of clinical samples.

3 Week 3 General methods of laboratory diagnosis - cultural, biochemical serological and

molecular methods

4 Week 3 Tests for antimicrobial susceptibility

Feb - 19

1 Week 3 Antiviral agents-interferons Host pathogen interactions.bacterial toxins

2 Week 3 Elements of chemotherapy,mode of action of penicillin

3 Week 3 Prevention and control of diseases& vaccines

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4 Week 3 Airborne diseases-Tuberculosis,food borne diseases-cholera,typhoid

Mar - 23

1 Week 3 Insect borne diseases-Malaria,dengue fever

2 Week 3 Contact and zoonotic diseases,blood borne diseases

3 Week Semester-end Examinations

4 Week

Signature of the Lecturer Signature of the HOD Signature of the Principal

Page 59: DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS) (A … · 2 Week 4 Preservation of microbial cultures –subculturing, overlaying cultures with mineral oils ... 3 Principles of food preservation

DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS)

(A College with Potential for Excellence)

Bhimavaram, W.G.Dist, A.P

Annual Curricular Plan for the Academic Year 2015-16

Department : U.G MICROBIOLOGY Paper : 4B Food and Industrial Microbiology Class: III B.Sc

Semester:VI

Month & No.of

Working Days Week

No.of Periods

Per week Topics to be covered Co-Curricular Activity

Nov - 13 3 Week 3 Microbiology of food spoilage and their sources

4 Week 3 Microbial spoilage of food - fruits, vegetables, milk, meat,

Dec - 24

1 Week 3

egg, bread and canned foods

2 Week 3 Food intoxication (botulism).

Food-borne diseases (salmonellosis) and their detection.

3 Week 3 Principles of food preservation - Physical and chemical methods.

4 Week 3 Fermented Dairy foods – cheese and yogurt.

Jan - 17

1 Week 3 Microorganisms as food – SCP, edible mushrooms

2 Week 3 white button, oyster and paddy straw

3 Week 3 Probiotics and their benefits

4 Week 3 Microorganisms of industrial importance – yeasts,(Saccharomyces cerevisiae) moulds

Feb - 19

1 Week 3 Aspergillus niger,Bacteria(E.coli), actinomycetes (Streptomyces griseus).

2 Week 3 Outlines of Isolation and Screening and strain improvement of industrially-important

microorganisms.

3 Week 3 Types of fermentation processes – solid state, liquid state, batch, fed-batch, continuous

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4 Week

3 Basic concepts of Design of fermenter.

Ingredients of Fermentation media

Mar - 23

1 Week 3 Microbial production of Industrial products - Citric acid

Ethanol, amylases, penicillin,

2 Week 3 glutamic acid and vitamin B12.

3 Week Semester-end Examinations

4 Week

Signature of the Lecturer Signature of the HOD Signature of the Principal

Page 61: DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS) (A … · 2 Week 4 Preservation of microbial cultures –subculturing, overlaying cultures with mineral oils ... 3 Principles of food preservation

DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS)

(A College with Potential for Excellence)

Bhimavaram, W.G.Dist, A.P

Annual Curricular Plan for the Academic Year 2014-15

Department :U.G MICROBIOLOGY Paper :IA INTRODUCTORY MICROBIOLOGY Class:I

B.Sc Semester:I

Month & No.of

Working Days Week

No.of Periods

Per week Topics to be covered Co-Curricular Activity

June - 24

1 Week 4 History of microbiology and Microscopy,Meaning definition and history of

Microbiology

2 Week 4

Contribution of antony van leeuwenhoek,Edward jenner,Louis pasteur

3 Week 4

Robert Koch,Iwanowsky,Beijerinck,winogradsky and Alexander flemming

4 Week 4

Applications of microbiology

July - 25

1 Week 4 Bright field,dark field,phase-contrast,fluorescent

2 Week 4 Electron microscopy(SEM AND TEM).Ocular and stage micrometers

3 Week 4 Principles and types of stains-simple stain,differential stain,negative stain

4 Week 4 Structural stains-spore ,capsule,flagella,hanging drop method

Aug - 21

1 Week 4 Sterilization and disinfection techniques

2 Week 4 Principles and methods sterilization.physical methods of sterilization

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3 Week 4 Radiation methods –uv rays,gamma rays,ultrasonic methods

4 Week 4 Chemical methods of sterilization –alcohols ,aldehydes,fumigants

Sept - 23

1 Week 4 Halogens ,hypochlorites.phenol coefficient

2 Week 4 Isolation of pure culture techniques-enrichment culturing dilution plating

3 Week 4 Streak plate,spread plate and micromanipulator.

4 Week 4 Preservation of microbial cultures,subculturing,overlaying cultures with mineral oils

Oct- 19

1 Week 4 Lyophilisation,sand cultures

2 Week 4 Storage at low temperatures.

3 Week Semester-end Examinations

4 Week

Nov- 10 1 Week

Semester-end Examinations

2 Week

Signature of the Lecturer Signature of the HOD Signature of the Principal

Page 63: DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS) (A … · 2 Week 4 Preservation of microbial cultures –subculturing, overlaying cultures with mineral oils ... 3 Principles of food preservation

DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS)

(A College with Potential for Excellence)

Bhimavaram, W.G.Dist, A.P

Annual Curricular Plan for the Academic Year 2014-15

Department :U.G MICROBIOLOGY Paper : 2A MICROBIAL PHYSIOLOGY AND GENETICS Class:

II B.Sc Semester:III

Month & No.of

Working Days Week

No.of Periods

Per week Topics to be covered Co-Curricular Activity

June - 24

1 Week 4 Nutritional requirements and uptake of nutrients by cells

2 Week 4

Nutritional groups of Microorganisms

3 Week 4

Autotrophs, heterotrophs, Mixotrophs,Methylotrophs

4 Week 4

Different phases of growth in batch culture

July - 25

1 Week 4 Factors influencing microbial growth

2 Week 4 Synchronous and continuous growth

3 Week 4 Biphasic growth, methods of measuring

4 Week 4 Microbial growth-direct microscopy

Aug - 21

1 Week 4 Viable count estimates,turbidometry,biomass

2 Week 4 Enzymes-properties and classification

3 Week 4 Enzyme unit ,bioatalysis-induced fit, lock and key model

4 Week 4 Coenzymes and cofactors

Sept - 23 1 Week 4 Factors affecting catalytic activity

Page 64: DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS) (A … · 2 Week 4 Preservation of microbial cultures –subculturing, overlaying cultures with mineral oils ... 3 Principles of food preservation

2 Week 4 Inhibition of Enzyme activity-competitive,uncompetitive and

allosteric

3 Week 4 Glycolysis,HMP Pathway,ED Pathway

4 Week 4 Electron transport,Oxidative and substrate level phosphorylation

Oct- 19

1 Week 4 Anaplerotic reactions,glyoxylate cycle

2 Week 4 Fermentaion and oxygenic and anoxygenic photosynthesis

3 Week Semester-end Examinations

4 Week

Nov- 10 1 Week

Semester-end Examinations

2 Week

Signature of the Lecturer Signature of the HOD Signature of the Principal

Page 65: DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS) (A … · 2 Week 4 Preservation of microbial cultures –subculturing, overlaying cultures with mineral oils ... 3 Principles of food preservation

DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS)

(A College with Potential for Excellence)

Bhimavaram, W.G.Dist, A.P

Annual Curricular Plan for the Academic Year 2014-15

Department :U.G MICROBIOLOGY Paper : 3A IMMUNOLOGY Class: III B.Sc

Semester:V

Month & No.of

Working Days Week

No.of Periods

Per week Topics to be covered Co-Curricular Activity

June - 24

1 Week 3 Types of immunity – innate and acquired; active and passive; humoral and cell-

mediated immunity

2 Week 3 Primary and secondary organs of immune system – thymus, bursa fabricus,

3 Week 3 bone marrow, spleen and lymph nodes.

4 Week 3 Identiification and function of B and T lymphocytes

July - 25

1 Week 3 null cells, monocytes, macrophages,

2 Week 3 neutrophils, basophils and eosinophils.

3 Week 3 Antigens – types, chemical nature, antigenic determinants, haptens.

Factors affecting antigenicity.

4 Week 3 Antibodies – basic structure, types,

Aug - 21

1 Week 3 properties and functions of immunoglobulins

2 Week 3 Types of antigen-antibody reactions - Agglutinations, Precipitation,

3 Week 3 Neutralization, complement fixation, blood groups.

4 Week 3 Labeled antibody based techniques

Sept - 23

1 Week 3 ELISA, RIA

2 Week 3 Immunofluroscence.

3 Week 3 Polyclonal& monoclonal antibodies

4 Week Monoclonal antibodies – production and applications.

Page 66: DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS) (A … · 2 Week 4 Preservation of microbial cultures –subculturing, overlaying cultures with mineral oils ... 3 Principles of food preservation

Oct- 19

1 Week 3 Types of hypersensitivity-immediate&delayed

2 Week 3 Autoimmunity& its significance

3 Week Semester-end Examinations

4 Week

Nov- 10 1 Week

Semester-end Examinations

2 Week

Signature of the Lecturer Signature of the HOD Signature of the Principal

Page 67: DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS) (A … · 2 Week 4 Preservation of microbial cultures –subculturing, overlaying cultures with mineral oils ... 3 Principles of food preservation

DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS)

(A College with Potential for Excellence)

Bhimavaram, W.G.Dist, A.P

Annual Curricular Plan for the Academic Year 2014-15

Department :U.G MICROBIOLOGY Paper :4A ENVIRONMENTAL MICROBIOLOGY Class:

III B.Sc Semester: V

Month & No.of

Working Days Week

No.of Periods

Per week Topics to be covered Co-Curricular Activity

June - 24

1 Week 3

Physical & chemical characteristics of soil

2 Week 3

Rhizosphere and phyllosphere

3 Week 3

Plant growth promoting microorganisms

4 Week 3

Outlines of biological nitrogen fixation(symbiotic& non symbiotic)

July - 25

1 Week 3 Biofertilizers&concept of diseases in plants

2 Week 3 Symptoms of plant diseases caused by fungi,bacteria

3 Week 3 Principles of plant disease control

4 Week 3 Biological control of plant diseases

Aug - 21

1 Week 3 Biopesticides-bt,NPV

2 Week 3 Microorganisms of environment

3 Week 3 Role of microorganisms in nutrient cycling

Page 68: DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS) (A … · 2 Week 4 Preservation of microbial cultures –subculturing, overlaying cultures with mineral oils ... 3 Principles of food preservation

4 Week 3 Microbial interactions-Mutualism,commensalism,antagonism

Sept - 23

1 Week 3 Microbiology of potable and polluted water

2 Week 3 Sanitation of potable water

3 Week 3 Sewage treatment(primary,secondary,tertiary)

4 Week 3 Outlines of biodegradation of environmental pollutants

Oct- 19

1 Week 3 Solid waste disposal ,sanitary land fills,composting

2 Week 3 Microbiology of air and air sampling methods

3 Week Semester-end Examinations

4 Week

Nov- 10 1 Week

Semester-end Examinations

2 Week

Signature of the Lecturer Signature of the HOD Signature of the Principal

Page 69: DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS) (A … · 2 Week 4 Preservation of microbial cultures –subculturing, overlaying cultures with mineral oils ... 3 Principles of food preservation

DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS)

(A College with Potential for Excellence)

Bhimavaram, W.G.Dist, A.P

Annual Curricular Plan for the Academic Year 2014-15

Department :U.G MICROBIOLOGY Paper : 1B BIOLOGY OF PROKARYOTIC AND EUKARYOTIC ORGANISMS

Class: I B.Sc Semester:II

Month & No.of

Working Days Week

No.of Periods

Per week Topics to be covered Co-Curricular Activity

Nov - 13

3 Week 4 Outline classification of living organisms,Haeckel ,whittaker carl woese systems

4 Week 4 Differentiation of prokaryotes and eukaryotes,general characteristics of bacteria

Dec - 24

1 Week 4 Archaebacteria,rickettsias ,mycoplasma,cynobacteria and actinomycetes

2 Week 4 Outline classification of bacteria as per the second edition of bergeys manual

3 Week 4 Ultra structure of bacterial cell, invariants and variants copmonents

4 Week 4 General characterisric and classification of viruses, morphology of TMV & HIV

Jan - 17

1 Week 4 General characterisric and classification of Eukaryotic microorganisms

2 Week 4 Outline classification and general characteristics of carbohydrates

3 Week 4 General characteristics of proteins

4 Week 4 Structure of nitrogenous bases,nucleotides,nucleic acids

Feb - 22

1 Week 4 Fatty acids (saturated and unsaturated)

2 Week 4 Lipids (spingolipids,sterols and phospholipids)

3 Week 4 Hydrogen ion concentration in biological fluids

Page 70: DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS) (A … · 2 Week 4 Preservation of microbial cultures –subculturing, overlaying cultures with mineral oils ... 3 Principles of food preservation

4 Week 4 Types of Buffers and their use in biological reactions

Mar - 22

1 Week 4 Principle and application of colorimeter

2 Week 4 Chromatography(paper and Thin layer)

3 Week Semester-end Examinations

4 Week

Signature of the Lecturer Signature of the HOD Signature of the Principal

Page 71: DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS) (A … · 2 Week 4 Preservation of microbial cultures –subculturing, overlaying cultures with mineral oils ... 3 Principles of food preservation

DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS)

(A College with Potential for Excellence)

Bhimavaram, W.G.Dist, A.P

Annual Curricular Plan for the Academic Year 2014-15

Department :U.G MICROBIOLOGY Paper :2B MOLECULAR BIOLOGY Class: II B.Sc

Semester:III

Month & No.of

Working Days Week

No.of Periods

Per week Topics to be covered Co-Curricular Activity

Nov - 13

3 Week 4 DNA & RNA as genetic materials.structure of DNA

4 Week 4 Extra chromosomal genetic elements-plasmids and transposons

Dec - 24

1 Week 4 Replication of DNA,Proteins involved in DNA Replication

2 Week 4 Outlines of Damage and Repair mechanism

3 Week 4 Spontaneous and Induced base pair changes

4 Week 4 Frame shifts deletions,invertions,tandem duplications

Jan - 17

1 Week 4 Various physical and chemical mutagens

2 Week 4 Gene transfer mechanisms in bacteria-transformation,transduction and conjugation

3 Week 4 Concept of gene –muton,recon and cistron

4 Week 4 One gene-one enzyme,one gene- one polypeptide

Feb - 22

1 Week 4 Types of RNA and their functions

2 Week 4 Genetic code –structure of , ribosomes and protein synthesis,operon concept

3 Week 4 Regulation of gene expression in bacteria,lac operon

Page 72: DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS) (A … · 2 Week 4 Preservation of microbial cultures –subculturing, overlaying cultures with mineral oils ... 3 Principles of food preservation

4 Week 4 Basic principles of genetic engineering-restriction endonucleases

Mar - 22

1 Week 4 DNA polymerases,ligases,vectors,outlines of gene cloning methods

2 Week 4 General account on applications of genetic engineering.

3 Week Semester-end Examinations

4 Week

Signature of the Lecturer Signature of the HOD Signature of the Principal

Page 73: DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS) (A … · 2 Week 4 Preservation of microbial cultures –subculturing, overlaying cultures with mineral oils ... 3 Principles of food preservation

DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS)

(A College with Potential for Excellence)

Bhimavaram, W.G.Dist, A.P

Annual Curricular Plan for the Academic Year 2014-15

Department :U.G MICROBIOLOGY Paper :3B MEDICAL MICROBIOLOGY Class: III B.Sc

Semester:VI

Month & No.of

Working Days Week

No.of Periods

Per week Topics to be covered Co-Curricular Activity

Nov - 13

3 Week 3 History of Medical microbiology

4 Week 3 Normal flora of human body

Dec - 24

1 Week 3 Definition of infection,non-specific defence mechanism

2 Week 3 Mechanical barriers,antagonism of indigenous flora

3 Week 3 Antibacterial substances-lysozyme,complement

4 Week 3 Properdin,antiviral substances,phagocytosis

Jan - 17

1 Week 3 General principles of diagnostic microbiology

2 Week 3 collection, transport and processing of clinical samples.

3 Week 3 General methods of laboratory diagnosis - cultural, biochemical serological and

molecular methods

4 Week 3 Tests for antimicrobial susceptibility

Feb - 22

1 Week 3 Antiviral agents-interferons Host pathogen interactions.bacterial toxins

2 Week 3 Elements of chemotherapy,mode of action of penicillin

Page 74: DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS) (A … · 2 Week 4 Preservation of microbial cultures –subculturing, overlaying cultures with mineral oils ... 3 Principles of food preservation

3 Week 3 Prevention and control of diseases& vaccines

4 Week 3 Airborne diseases-Tuberculosis,food borne diseases-cholera,typhoid

Mar - 22

1 Week 3 Insect borne diseases-Malaria,dengue fever

2 Week 3 Contact and zoonotic diseases,blood borne diseases

3 Week Semester-end Examinations

4 Week

Signature of the Lecturer Signature of the HOD Signature of the Principal

Page 75: DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS) (A … · 2 Week 4 Preservation of microbial cultures –subculturing, overlaying cultures with mineral oils ... 3 Principles of food preservation

DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS)

(A College with Potential for Excellence)

Bhimavaram, W.G.Dist, A.P

Annual Curricular Plan for the Academic Year 2014-15

Department :U.G MICROBIOLOGY Paper : 4B FOOD&INDUSTRIAL MICROBIOLOGY Class: III B.Sc

Semester:VI

Month & No.of

Working Days Week

No.of Periods

Per week Topics to be covered Co-Curricular Activity

Nov - 13

3 Week 3

Microbiology of food spoilage and their sources

4 Week 3

Microbial spoilage of food - fruits, vegetables, milk, meat,

Dec - 24

1 Week 3

egg, bread and canned foods

2 Week 3 Food intoxication (botulism).

Food-borne diseases (salmonellosis) and their detection.

3 Week 3 Principles of food preservation - Physical and chemical methods.

4 Week 3 Fermented Dairy foods – cheese and yogurt.

Jan - 17

1 Week 3 Microorganisms as food – SCP, edible mushrooms

2 Week 3 white button, oyster and paddy straw

3 Week 3 Probiotics and their benefits

4 Week 3 Microorganisms of industrial importance – yeasts,(Saccharomyces cerevisiae) moulds

Feb - 22

1 Week 3 Aspergillus niger,Bacteria(E.coli), actinomycetes (Streptomyces griseus).

2 Week 3 Outlines of Isolation and Screening and strain improvement of industrially-important

microorganisms.

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3 Week 3 Types of fermentation processes – solid state, liquid state, batch, fed-batch, continuous

4 Week

3 Basic concepts of Design of fermenter.

Ingredients of Fermentation media

Mar - 22

1 Week 3 Microbial production of Industrial products - Citric acid

Ethanol, amylases, penicillin,

2 Week 3 glutamic acid and vitamin B12.

3 Week Semester-end Examinations

4 Week

Signature of the Lecturer Signature of the HOD Signature of the Principal

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DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS)

(A College with Potential for Excellence)

Bhimavaram, W.G.Dist, A.P

Annual Curricular Plan for the Academic Year 2013-14

Department :U.G MICROBIOLOGY Paper : 1A INTRODUCTORY MICROBIOLOGY Class:I B.Sc

Semester:I

Month & No.of

Working Days Week

No.of Periods

Per week Topics to be covered Co-Curricular Activity

June - 10

1 Week History of microbiology and Microscopy,Meaning definition and history of

Microbiology

2 Week 4 Contribution of antony van leeuwenhoek,Edward jenner,Louis pasteur

3 Week 4

Robert Koch,Iwanowsky,Beijerinck,winogradsky and Alexander flemming

4 Week 4

Applications of microbiology

July - 26

1 Week 4 Bright field,dark field,phase-contrast,fluorescent

2 Week 4 Electron microscopy(SEM AND TEM).Ocular and stage micrometers

3 Week 4 Principles and types of stains-simple stain,differential stain,negative stain

4 Week 4 Structural stains-spore ,capsule,flagella,hanging drop method

Aug - 21

1 Week 4 Sterilization and disinfection techniques

2 Week 4 Principles and methods sterilization.physical methods of sterilization

3 Week 4 Radiation methods –uv rays,gamma rays,ultrasonic methods

4 Week 4 Chemical methods of sterilization –alcohols ,aldehydes,fumigants

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Sept - 23

1 Week 4 Halogens ,hypochlorites.phenol coefficient

2 Week 4 Isolation of pure culture techniques-enrichment culturing dilution plating

3 Week 4 Streak plate,spread plate and micromanipulator.

4 Week 4 Preservation of microbial cultures,subculturing,overlaying cultures with mineral oils

Oct- 26

1 Week 4 Lyophilisation,sand cultures

2 Week 4 Storage at low temperatures.

3 Week Semester-end Examinations

4 Week

Nov- 10 1 Week

Semester-end Examinations

2 Week

Signature of the Lecturer Signature of the HOD Signature of the Principal

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DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS)

(A College with Potential for Excellence)

Bhimavaram, W.G.Dist, A.P

Annual Curricular Plan for the Academic Year 2013-14

Department :U.G MICROBIOLOGY Paper : 2A Microbial physiology and genetics Class:II B.Sc

Semester:III

Month & No.of

Working Days Week

No.of Periods

Per week Topics to be covered Co-Curricular Activity

June - 10

1 Week 4

Nutritional requirements and uptake of nutrients by cells

2 Week 4

Nutritional groups of Microorganisms

3 Week 4

Autotrophs, heterotrophs, Mixotrophs,Methylotrophs

4 Week 4

Different phases of growth in batch culture

July - 26

1 Week 4 Factors influencing microbial growth

2 Week 4 Synchronous and continuous growth

3 Week 4 Biphasic growth, methods of measuring

4 Week 4 Microbial growth-direct microscopy

Aug - 21

1 Week 4 Viable count estimates,turbidometry,biomass

2 Week 4 Enzymes-properties and classification

3 Week 4 Enzyme unit ,bioatalysis-induced fit, lock and key model

4 Week 4 Coenzymes and cofactors

Sept - 23 1 Week 4 Factors affecting catalytic activity

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2 Week 4 Inhibition of Enzyme activity-competitive,uncompetitive and

allosteric

3 Week 4 Glycolysis,HMP Pathway,ED Pathway

4 Week 4 Electron transport,Oxidative and substrate level phosphorylation

Oct- 26

1 Week 4 Anaplerotic reactions,glyoxylate cycle

2 Week 4 Fermentaion and oxygenic and anoxygenic photosynthesis

3 Week Semester-end Examinations

4 Week

Nov- 10 1 Week

Semester-end Examinations

2 Week

Signature of the Lecturer Signature of the HOD Signature of the Principal

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DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS)

(A College with Potential for Excellence)

Bhimavaram, W.G.Dist, A.P

Annual Curricular Plan for the Academic Year 2013-14

Department :U.G MICROBIOLOGY Paper : 3A IMMMUNOLOGY Class: III B.Sc

Semester: V

Month & No.of

Working Days Week

No.of Periods

Per week Topics to be covered Co-Curricular Activity

June - 10

1 Week 3 Types of immunity – innate and acquired; active and passive; humoral and cell-

mediated immunity

2 Week 3

Primary and secondary organs of immune system – thymus, bursa fabricus,

3 Week 3 bone marrow, spleen and lymph nodes.

4 Week 3

Identiification and function of B and T lymphocytes

July - 26

1 Week 3 null cells, monocytes, macrophages,

2 Week 3 neutrophils, basophils and eosinophils.

3 Week

3 Antigens – types, chemical nature, antigenic determinants, haptens.

Factors affecting antigenicity.

4 Week 3 Antibodies – basic structure, types,

Aug - 21

1 Week 3 properties and functions of immunoglobulins

2 Week 3 Types of antigen-antibody reactions - Agglutinations, Precipitation,

3 Week 3 Neutralization, complement fixation, blood groups.

4 Week 3 Labeled antibody based techniques

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Sept - 23

1 Week 3 ELISA, RIA

2 Week 3 Immunofluroscence.

3 Week 3 Polyclonal& monoclonal antibodies

4 Week 3 Monoclonal antibodies – production and applications.

Oct- 26

1 Week 3 Types of hypersensitivity-immediate&delayed

2 Week 3 Autoimmunity& its significance

3 Week Semester-end Examinations

4 Week

Nov- 10 1 Week

Semester-end Examinations

2 Week

Signature of the Lecturer Signature of the HOD Signature of the Principal

Page 83: DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS) (A … · 2 Week 4 Preservation of microbial cultures –subculturing, overlaying cultures with mineral oils ... 3 Principles of food preservation

DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS)

(A College with Potential for Excellence)

Bhimavaram, W.G.Dist, A.P

Annual Curricular Plan for the Academic Year 2013-14

Department :U.G MICROBIOLOGY Paper : 4A ENVIRONMENTAL MICROBIOLOGY Class: III B.Sc

Semester:V

Month & No.of

Working Days Week

No.of Periods

Per week Topics to be covered Co-Curricular Activity

June - 10

1 Week 3

Physical & chemical characteristics of soil

2 Week 3

Rhizosphere and phyllosphere

3 Week 3

Plant growth promoting microorganisms

4 Week 3

Outlines of biological nitrogen fixation(symbiotic& non symbiotic)

July - 26

1 Week 3 Biofertilizers&concept of diseases in plants

2 Week 3 Symptoms of plant diseases caused by fungi,bacteria

3 Week 3 Principles of plant disease control

4 Week 3 Biological control of plant diseases

Aug - 21

1 Week 3 Biopesticides-bt,NPV

2 Week 3 Microorganisms of environment

3 Week 3 Role of microorganisms in nutrient cycling

4 Week 3 Microbial interactions-Mutualism,commensalism,antagonism

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Sept - 23

1 Week 3 Microbiology of potable and polluted water

2 Week 3 Sanitation of potable water

3 Week 3 Sewage treatment(primary,secondary,tertiary)

4 Week 3 Outlines of biodegradation of environmental pollutants

Oct- 26

1 Week 3 Solid waste disposal ,sanitary land fills,composting

2 Week 3 Microbiology of air and air sampling methods

3 Week

Semester-end Examinations

4 Week 4

Nov- 10

1 Week 4

Semester-end Examinations

2 Week 4

Signature of the Lecturer Signature of the HOD Signature of the Principal

Page 85: DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS) (A … · 2 Week 4 Preservation of microbial cultures –subculturing, overlaying cultures with mineral oils ... 3 Principles of food preservation

DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS)

(A College with Potential for Excellence)

Bhimavaram, W.G.Dist, A.P

Annual Curricular Plan for the Academic Year 2013-14

Department :U.G MICROBIOLOGY Paper :1B BIOLOGY OF PROKARYOTIC AND EUKARYOTIC ORGANISMS

Class: I B.Sc Semester:II

Month & No.of

Working Days Week

No.of Periods

Per week Topics to be covered Co-Curricular Activity

Nov - 13

3 Week 4 Outline classification of living organisms,Haeckel ,whittaker carl woese systems

4 Week 4 Differentiation of prokaryotes and eukaryotes,general characteristics of bacteria

Dec - 23

1 Week 4 Archaebacteria,rickettsias ,mycoplasma,cynobacteria and actinomycetes

2 Week 4 Outline classification of bacteria as per the second edition of bergeys manual

3 Week 4 Ultra structure of bacterial cell, invariants and variants copmonents

4 Week 4 General characterisric and classification of viruses, morphology of TMV & HIV

Jan - 19

1 Week 4 General characterisric and classification of Eukaryotic microorganisms

2 Week 4 Outline classification and general characteristics of carbohydrates

3 Week 4 General characteristics of proteins

4 Week 4 Structure of nitrogenous bases,nucleotides,nucleic acids

Feb - 21

1 Week 4 Fatty acids (saturated and unsaturated)

2 Week 4 Lipids (spingolipids,sterols and phospholipids)

3 Week 4 Hydrogen ion concentration in biological fluids

4 Week 4 Types of Buffers and their use in biological reactions

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Mar - 22

1 Week 4 Principle and application of colorimeter

2 Week 4 Chromatography(paper and Thin layer)

3 Week 4

Semester-end Examinations

4 Week 4

Signature of the Lecturer Signature of the HOD Signature of the Principal

Page 87: DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS) (A … · 2 Week 4 Preservation of microbial cultures –subculturing, overlaying cultures with mineral oils ... 3 Principles of food preservation

DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS)

(A College with Potential for Excellence)

Bhimavaram, W.G.Dist, A.P

Annual Curricular Plan for the Academic Year 2013-14

Department :U.G MICROBIOLOGY Paper :2B MOLECULAR BIOLOGY Class: II B.Sc

Semester:IV

Month & No.of

Working Days Week

No.of Periods

Per week Topics to be covered Co-Curricular Activity

Nov - 13

3 Week 4 DNA & RNA as genetic materials.structure of DNA

4 Week 4 Extra chromosomal genetic elements-plasmids and transposons

Dec - 23

1 Week 4 Replication of DNA,Proteins involved in DNA Replication

2 Week 4 Outlines of Damage and Repair mechanism

3 Week 4 Spontaneous and Induced base pair changes

4 Week 4 Frame shifts deletions,invertions,tandem duplications

Jan - 19

1 Week 4 Various physical and chemical mutagens

2 Week 4 Gene transfer mechanisms in bacteria-transformation,transduction and conjugation

3 Week 4 Concept of gene –muton,recon and cistron

4 Week 4 One gene-one enzyme,one gene- one polypeptide

Feb - 21

1 Week 4 Types of RNA and their functions

2 Week 4 Genetic code –structure of , ribosomes and protein synthesis,operon concept

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3 Week 4 Regulation of gene expression in bacteria,lac operon

4 Week 4 Basic principles of genetic engineering-restriction endonucleases

Mar - 22

1 Week 4 DNA polymerases,ligases,vectors,outlines of gene cloning methods

2 Week 4 General account on applications of genetic engineering.

3 Week

Semester-end Examinations

4 Week

Signature of the Lecturer Signature of the HOD Signature of the Principal

Page 89: DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS) (A … · 2 Week 4 Preservation of microbial cultures –subculturing, overlaying cultures with mineral oils ... 3 Principles of food preservation

DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS)

(A College with Potential for Excellence)

Bhimavaram, W.G.Dist, A.P

Annual Curricular Plan for the Academic Year 2013-14

Department :U.G MICROBIOLOGY Paper : 3B MEDICAL MICROBIOLOGY Class:III B.Sc

Semester:VI

Month & No.of

Working Days Week

No.of Periods

Per week Topics to be covered Co-Curricular Activity

Nov - 13

3 Week 3 History of Medical microbiology

4 Week 3 Normal flora of human body

Dec - 23

1 Week 3 Definition of infection,non-specific defence mechanism

2 Week 3 Mechanical barriers,antagonism of indigenous flora

3 Week 3 Antibacterial substances-lysozyme,complement

4 Week 3 Properdin,antiviral substances,phagocytosis

Jan - 19

1 Week 3 General principles of diagnostic microbiology

2 Week 3 collection, transport and processing of clinical samples.

3 Week 3 General methods of laboratory diagnosis - cultural, biochemical serological and

molecular methods

4 Week 3 Tests for antimicrobial susceptibility

Feb - 21

1 Week 3 Antiviral agents-interferons Host pathogen interactions.bacterial toxins

2 Week 3 Elements of chemotherapy,mode of action of penicillin

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3 Week 3 Prevention and control of diseases& vaccines

4 Week 3 Airborne diseases-Tuberculosis,food borne diseases-cholera,typhoid

Mar - 22

1 Week 3 Insect borne diseases-Malaria,dengue fever

2 Week 3 Contact and zoonotic diseases,blood borne diseases

3 Week

Semester-end Examinations

4 Week 4

Signature of the Lecturer Signature of the HOD Signature of the Principal

Page 91: DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS) (A … · 2 Week 4 Preservation of microbial cultures –subculturing, overlaying cultures with mineral oils ... 3 Principles of food preservation

DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS)

(A College with Potential for Excellence)

Bhimavaram, W.G.Dist, A.P

Annual Curricular Plan for the Academic Year 2013-14

Department :U.G MICROBIOLOGY Paper : 4B FOOD & INDUSTRIAL MICROBIOLOGY Class: III B.Sc

Semester:VI

Month & No.of

Working Days Week

No.of Periods

Per week Topics to be covered Co-Curricular Activity

Nov - 13

3 Week 3 Microbiology of food spoilage and their sources

4 Week 3

Microbial spoilage of food - fruits, vegetables, milk, meat,

Dec - 23

1 Week 3

egg, bread and canned foods

2 Week 3 Food intoxication (botulism).

Food-borne diseases (salmonellosis) and their detection.

3 Week 3 Principles of food preservation - Physical and chemical methods.

4 Week 3 Fermented Dairy foods – cheese and yogurt.

Jan - 19

1 Week 3 Microorganisms as food – SCP, edible mushrooms

2 Week 3 white button, oyster and paddy straw

3 Week 3 Probiotics and their benefits

4 Week 3 Microorganisms of industrial importance – yeasts,(Saccharomyces cerevisiae) moulds

Feb - 21

1 Week 3 Aspergillus niger,Bacteria(E.coli), actinomycetes (Streptomyces griseus).

2 Week 3 Outlines of Isolation and Screening and strain improvement of industrially-important

microorganisms.

3 Week 3 Types of fermentation processes – solid state, liquid state, batch, fed-batch, continuous

Page 92: DANTULURI NARAYANA RAJU COLLEGE(AUTONOMOUS) (A … · 2 Week 4 Preservation of microbial cultures –subculturing, overlaying cultures with mineral oils ... 3 Principles of food preservation

4 Week

3 Basic concepts of Design of fermenter.

Ingredients of Fermentation media

Mar - 22

1 Week 3 Microbial production of Industrial products - Citric acid

Ethanol, amylases, penicillin,

2 Week 3 glutamic acid and vitamin B12.

3 Week Semester-end Examinations

4 Week

Signature of the Lecturer Signature of the HOD Signature of the Principal