damage reduction via scanning electron microscopy · 2018-03-15 · damage reduction via scanning...
TRANSCRIPT
Kosmoscience Ciência & Tecnologia Cosmética Ltda
www.kosmoscience.com
TR-B&R007-18-A-R2, Page 1 of 42, March 13, 2018. Confidential Document.
TEST REPORT B&R007-18-A, Review 2
Test Proposal B&R007-18, Review 1
Damage Reduction via Scanning Electron Microscopy
Products Evaluated
Monat Renew Shampoo
Monat Restore Leave-in Conditioner
Monat Volume Revive Shampoo
Monat Volume Revitalize Conditioner
Monat Black Shampoo + Conditioner
Sponsor: MONAT GLOBAL
Thomas J. Hoolihan
Senior Vice President and Chief Legal Officer
3470 N.W. 82nd Avenue, Suite 910. Miami, FL – USA 33122
TR-B&R007-18-A-R2, Page 2 of 42, March 13, 2018. Confidential Document.
SYNOPSIS OF TEST REPORT TR-B&R007-18-A-R2
Study Code: B&R007-18
Title of Study: Damage Reduction via Scanning Electron Microscopy.
Product Evaluated: Monat Renew Shampoo; Monat Restore Leave-in Conditioner; Monat Volume Revive Shampoo;
Monat Volume Revitalize Conditioner; Monat Black Shampoo + Conditioner.
Study plan code: PE-B&R007-18-R0
Purpose of study: The purpose of this study was to evaluate levels of damage to the surface of hair fibers submitted
to treatment with cosmetic products via scanning electron microscopy (SEM).
Study Director: Viviane C. Albarici
Chronology of study: The study was carried out from February 20th to March 2nd, 2018.
Experimental Design: In the present study, tresses of natural and bleached/dyed Caucasian hair were submitted 12
applications of the treatments as described below:
Virgin Caucasian Hair - group Virgin;
Bleached and Dyed Hair – group Bleached/Dyed;
Virgin Hair + Monat Renew Shampoo (Normal Condition) – group T01;
Virgin Hair + Monat Renew Shampoo (Modified Condition) – group T02;
Virgin Hair + Monat Renew Shampoo (Normal Condition) + Flat Iron – group T03;
Bleached and Dyed Hair + Monat Renew Shampoo (Normal Condition) – group T04;
Bleached and Dyed Hair + Monat Renew Shampoo (Modified Condition) – group T05;
Bleached and Dyed Hair + Monat Renew Shampoo (Normal Condition) + Flat Iron – group
T06;
Virgin Hair + Monat Volume Revive Shampoo (Normal Condition) – group T07;
Virgin Hair + Monat Volume Revive Shampoo (Modified Condition) – group T08;
Virgin Hair + Monat Volume Revive Shampoo (Normal Condition) + Flat Iron – group T09;
Bleached and Dyed Hair + Monat Volume Revive Shampoo (Normal Condition) – group T10;
Bleached and Dyed Hair + Monat Volume Revive Shampoo (Modified Condition) – group
T11;
Bleached and Dyed Hair + Monat Volume Revive Shampoo (Normal Condition) + Flat Iron
– group T12;
Virgin Hair + Monat Black Shampoo + Conditioner (Normal Condition) – group T13;
Virgin Hair + Monat Black Shampoo + Conditioner (Modified Condition) – group T14;
Virgin Hair + Monat Black Shampoo + Conditioner (Normal Condition) + Flat Iron – group
T15;
Bleached and Dyed Hair + Monat Black Shampoo + Conditioner (Normal Condition) –
group T16;
Bleached and Dyed Hair + Monat Black Shampoo + Conditioner (Modified Condition) –
group T17;
Bleached and Dyed Hair + Monat Black Shampoo + Conditioner (Normal Condition) + Flat
Iron – group T18;
TR-B&R007-18-A-R2, Page 3 of 42, March 13, 2018. Confidential Document.
Virgin Hair + Monat Renew Shampoo + Monat Restore Leave-in Conditioner (Normal
Condition) – group T19;
Virgin Hair + Monat Renew Shampoo + Monat Restore Leave-in Conditioner (Modified
Condition) – group T20;
Virgin Hair + Monat Renew Shampoo + Monat Restore Leave-in Conditioner (Normal
Condition) + Flat Iron – group T21;
Virgin Hair + Monat Renew Shampoo + Monat Restore Leave-in Conditioner (Modified
Condition) + Flat Iron – group T22;
Bleached and Dyed Hair + Monat Renew Shampoo + Monat Restore Leave-in Conditioner
(Normal Condition) – group T23;
Bleached and Dyed Hair + Monat Renew Shampoo + Monat Restore Leave-in Conditioner
(Modified Condition) – group T24;
Bleached and Dyed Hair + Monat Renew Shampoo + Monat Restore Leave-in Conditioner
(Normal Condition) + Flat Iron – group T25;
Bleached and Dyed Hair + Monat Renew Shampoo + Monat Restore Leave-in Conditioner
(Modified Condition) + Flat Iron – group T26;
Virgin Hair + Monat Volume Revive Shampoo + Monat Volume Revitalize Conditioner
(Normal Condition) – group T27;
Virgin Hair + Monat Volume Revive Shampoo + Monat Volume Revitalize Conditioner
(Modified Condition) – group T28;
Virgin Hair + Monat Volume Revive Shampoo + Monat Volume Revitalize Conditioner
(Normal Condition) + Flat Iron – group T29;
Virgin Hair + Monat Volume Revive Shampoo + Monat Volume Revitalize Conditioner
(Modified Condition) + Flat Iron – group T30;
Bleached and Dyed Hair + Monat Volume Revive Shampoo + Monat Volume Revitalize
Conditioner (Normal Condition) – group T31;
Bleached and Dyed Hair + Monat Volume Revive Shampoo + Monat Volume Revitalize
Conditioner (Modified Condition) – group T32.
Bleached and Dyed Hair + Monat Volume Revive Shampoo + Monat Volume Revitalize
Conditioner (Normal Condition) + Flat Iron – group T33;
Bleached and Dyed Hair + Monat Volume Revive Shampoo + Monat Volume Revitalize
Conditioner (Modified Condition) + Flat Iron – group T34.
From the hair fibers collected, 5-mm long segments were taken from the center area. These
segments were fastened to the microscope sample holder using the conductive adhesive tape to
the carbon base. The samples were coated with a conductive layer of 90 Å Gold using the Spulter
Balzers SCD-050 Coater. For each treatment group, 05 micrographs were randomly obtained,
using the ZEISS™ 940-A scanning electron microscope at 15 kV.
Directions: Shampoo (Normal Condition): Apply 0,6 mL of Shampoo and rub the tresses for 30 seconds.
Leave it on hair for 3 minutes. Rinse for 30 seconds and remove excess water.
TR-B&R007-18-A-R2, Page 4 of 42, March 13, 2018. Confidential Document.
Shampoo (Modified Condition): Apply 0,3 mL of Shampoo and rub the tresses for 30 seconds.
Rinse for 30 seconds and remove excess water. Repeat the wash.
Conditioner: Apply 0.45 mL of Conditioner and rub it for 60 seconds. Rinse the tress for 30
seconds and remove excess water.
Conclusion: Virgin Hair
According to the results, the tresses submitted to 12 cycles of treatments using “Monat Renew
Shampoo”, “Monat Volume Revive Shampoo”, “Monat Black Shampoo + Conditioner”, “Monat
Renew Shampoo + Monat Restore Leave-in Conditioner” and “Monat Volume Revive Shampoo
+ Monat Volume Revitalize Conditioner”, on Normal or Modified conditions showed no effect on
surface damage of natural hair.
Moreover, the tresses submitted to these treatments following flat iron application showed no
effect on surface damage of natural hair, either.
Bleached/Dyed Hair
According to the results, the tresses submitted to 12 cycles of treatments using “Monat Renew
Shampoo”, “Monat Volume Revive Shampoo”, “Monat Black Shampoo + Conditioner”, “Monat
Renew Shampoo + Monat Restore Leave-in Conditioner” and “Monat Volume Revive Shampoo
+ Monat Volume Revitalize Conditioner”, on Normal or Modified conditions showed no effect on
surface damage of bleached and dyed hair.
Furthermore, the tresses submitted to these treatments following flat iron application showed no
effect on surface damage of bleached and dyed hair ether.
Finaly, the the products “Monat Renew Shampoo + Monat Restore Leave-in Conditioner” and
“Monat Volume Revive Shampoo + Monat Volume Revitalize Conditioner” slightly contribuite to
improve smoothing of the cuticule when compared to the bleached and dyed hair.
The table below shows the Reduction of Damage, RD, calculated to the treatments T23, T24, T31
and T32:
“Reduction of Damage, RD”of the treatment group (in percentage and number of times) compared to the Bleached/Dyed group.
Study group % Number of
times
Bleached and Dyed Hair + Monat Renew Shampoo + Monat Restore Leave-in Conditioner (Normal Condition)
17 1.2
Bleached and Dyed Hair + Monat Renew Shampoo + Monat Restore Leave-in Conditioner (Modified Condition)
15 1.2
Bleached and Dyed Hair + Monat Volume Revive Shampoo + Monat Volume Revitalize Conditioner (Normal Condition)
15 1.2
Bleached and Dyed Hair + Monat Volume Revive Shampoo + Monat Volume Revitalize Conditioner (Modified Condition)
14 1.2
TR-B&R007-18-A-R2, Page 5 of 42, March 13, 2018. Confidential Document.
COPYRIGHT NOTICE
Copyright © 2018 Kosmoscience. All rights reserved.
This Study Report is a copyright material owned by the sponsor of the study and, therefore, all the rights herein are
reserved and protected by Law 9.610 dated 02/19/1998. This document will not be reproduced, whether totally or
partially, by any processes or means, and it will not be disclosed by any electronic means, such as the Internet, without
the prior written approval from Kosmoscience.
This document is authorized for internal use by the sponsor, and by government supervision agencies, including
ANVISA/Health Ministry for the purposes of enrolling the tested product.
The study Sponsor may mention the original document and make use of the reference results achieved in its own
published materials.
The test report may only be completely reproduced if an approval in writing by the lab is requested. In the same
way, the approval in writing should be requested when there be need the reproduction of the document parts, especially
including images or pictures.
The results and discussion described in this report refer only to tested items.
TR-B&R007-18-A-R2, Page 6 of 42, March 13, 2018. Confidential Document.
AMENDMENTS TO THE TEST REPORT
Test Report B&R007-18-A, Review “1”, issued on 03/13/2018, becomes obsolete after the issuance of
this report named: Test Report B&R007-18-A, Review “2” issued on 03/13/2018.
Any reproduction of data from the formerly issued Test Report B&R007-18-A, Review “1” becomes impractical and
invalid starting on the date of this new issuance.
TR-B&R007-18-A-R2, Page 7 of 42, March 13, 2018. Confidential Document.
CONTENTS
I – INTRODUCTION ........................................................................................................................ 8
II – OBJECTIVE ............................................................................................................................... 9
III – EXPERIMENTAL PROCEDURE ................................................................................................. 10
IV – RESULTS AND DISCUSSION ................................................................................................... 12
V – CONCLUSION ......................................................................................................................... 27
VI – REFERENCES ........................................................................................................................ 31
VII – HISTORY OF REVIEWS .......................................................................................................... 32
APPENDIX I – Formulation of the samples forwarded by the Sponsor ................................................... 33
APPENDIX II – Experimental Procedure ............................................................................................ 34
APPENDIX III – Experimental Data Obtained ..................................................................................... 36
APPENDIX IV – Statistical Analysis .................................................................................................. 37
TR-B&R007-18-A-R2, Page 8 of 42, March 13, 2018. Confidential Document.
I – INTRODUCTION
The scanning electron microscopy (SEM) is a technique that uses a beam of electrons providing images with
high resolution and high depth of field. This has been used, to characterize pathologies, to classify hair according to its
origin or age and for forensic studies. Besides, with this technique is possible to see and quantify changes on the surface
of hair fibers. Thus, the scanning electron microscopy technique allows the evaluation of the surface of the hair fibers about
to prevent and repair damage caused by straightening procedures, dyeing, bleaching, successive combability, exposure to
sun light and temperature (such as the use of dryers and flat iron).
The scanning electron microscopy (SEM) technique was used in this study to assess the surface of hair fibers
submitted to several hair care products. The image is the result of secondary electrons formed while the sample surface is
scanned by a high-intensity beam of electrons.
By means of image analysis software programs, it is possible to standardize images and quantify the
respective surface damage to enable a more precise comparison of the action of different hair care products.
TR-B&R007-18-A-R2, Page 9 of 42, March 13, 2018. Confidential Document.
II – OBJECTIVE
The purpose of this study is to evaluate levels of damage to the surface of hair fibers submitted to treatment
with cosmetic products via scanning electron microscopy (SEM).
TR-B&R007-18-A-R2, Page 10 of 42, March 13, 2018. Confidential Document.
III – EXPERIMENTAL PROCEDURE
1. Generalities
1.1 Study Plan
The study was conducted per guidelines of study plan PE-B&R007-18-R0.
1.2 Dates
The study was approved to start on February 20rd, 2018 and it was carried out from February 20th to March
2nd, 2018.
2. Identifying samples supplied by the Sponsor for the Study
A27-156 Monat Renew Shampoo – 18J0115107;
A27-044 Monat Volume Revive Shampoo – 18J0115104;
A27-040 Monat Volume Revitalize Conditioner – 17J1227153;
A17-062D Monat Black Shampoo + Conditioner – 18J0117146;
A27-040 Monat Volume Revitalize Conditioner – 17J1227153;
A27-042 Monat Restore Leave-In Conditioner – 18J0111087.
The formulations of the samples, forwarded by the Sponsor, are listed in Appendix I. After the study, all
residues of samples were filed per Kosmoscience® Quality Procedures under unique study identification code B&R007-
18 for a six-month period.
3. Procedure for conducting evaluations
Fifty four tresses were prepared from Natural Caucasian hair and Fifty four tresses were prepared from
bleached/dyed Caucasian hair, weighing 3.0 g each and 25 cm long. All tresses underwent a standard pre-cleaning
process with 10% Sodium lauryl ether sulfate (SLES) solution for 1 minute then rinsed with running water.
The tresses were split in 36 groups and submitted to 12 applications of the treatments, as described below:
Treatment Study Code
Virgin Caucasian Hair Virgin
Bleached and Dyed Hair Bleached/Dyed
Virgin Hair + Monat Renew Shampoo (Normal Condition) T01
Virgin Hair + Monat Renew Shampoo (Modified Condition) T02
Virgin Hair + Monat Renew Shampoo (Normal Condition) + Flat Iron T03
Bleached and Dyed Hair + Monat Renew Shampoo (Normal Condition) T04
Bleached and Dyed Hair + Monat Renew Shampoo (Modified Condition) T05
Bleached and Dyed Hair + Monat Renew Shampoo (Normal Condition) + Flat Iron T06
Virgin Hair + Monat Volume Revive Shampoo (Normal Condition) T07
Virgin Hair + Monat Volume Revive Shampoo (Modified Condition) T08
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Virgin Hair + Monat Volume Revive Shampoo (Normal Condition) + Flat Iron T09
Bleached and Dyed Hair + Monat Volume Revive Shampoo (Normal Condition) T10
Bleached and Dyed Hair + Monat Volume Revive Shampoo (Modified Condition) T11
Bleached and Dyed Hair + Monat Volume Revive Shampoo (Normal Condition) + Flat Iron T12
Virgin Hair + Monat Black Shampoo + Conditioner (Normal Condition) T13
Virgin Hair + Monat Black Shampoo + Conditioner (Modified Condition) T14
Virgin Hair + Monat Black Shampoo + Conditioner (Normal Condition)+ Flat Iron T15
Bleached and Dyed Hair + Monat Black Shampoo + Conditioner (Normal Condition) T16
Bleached and Dyed Hair + Monat Black Shampoo + Conditioner (Modified Condition) T17
Bleached and Dyed Hair + Monat Black Shampoo + Conditioner (Normal Condition) + Flat Iron T18
Virgin Hair + Monat Renew Shampoo + Monat Restore Leave-in Conditioner (Normal Condition) T19
Virgin Hair + Monat Renew Shampoo + Monat Restore Leave-in Conditioner (Modified Condition) T20
Virgin Hair + Monat Renew Shampoo + Monat Restore Leave-in Conditioner (Normal Condition) + Flat Iron
T21
Virgin Hair + Monat Renew Shampoo + Monat Restore Leave-in Conditioner (Modified Condition) + Flat Iron
T22
Bleached and Dyed Hair + Monat Renew Shampoo + Monat Restore Leave-in Conditioner (Normal Condition)
T23
Bleached and Dyed Hair + Monat Renew Shampoo + Monat Restore Leave-in Conditioner (Modified Condition)
T24
Bleached and Dyed Hair + Monat Renew Shampoo + Monat Restore Leave-in Conditioner (Normal Condition) + Flat Iron
T25
Bleached and Dyed Hair + Monat Renew Shampoo + Monat Restore Leave-in Conditioner (Modified Condition) + Flat Iron
T26
Virgin Hair + Monat Volume Revive Shampoo + Monat Volume Revitalize Conditioner (Normal Condition)
T27
Virgin Hair + Monat Volume Revive Shampoo + Monat Volume Revitalize Conditioner (Modified Condition)
T28
Virgin Hair + Monat Volume Revive Shampoo + Monat Volume Revitalize Conditioner (Normal Condition) + Flat Iron
T29
Virgin Hair + Monat Volume Revive Shampoo + Monat Volume Revitalize Conditioner (Modified Condition) + Flat Iron
T30
Bleached and Dyed Hair + Monat Volume Revive Shampoo + Monat Volume Revitalize Conditioner (Normal Condition)
T31
Bleached and Dyed Hair + Monat Volume Revive Shampoo + Monat Volume Revitalize Conditioner (Modified Condition)
T32
Bleached and Dyed Hair + Monat Volume Revive Shampoo + Monat Volume Revitalize Conditioner (Normal Condition) + Flat Iron
T33
Bleached and Dyed Hair + Monat Volume Revive Shampoo + Monat Volume Revitalize Conditioner (Modified Condition) + Flat Iron
T34
After the treatment, the hair fibers were collected for SEM analysis, 24 hours of drying in a controlled environment
at 55 ± 5% relative humidity and 22 ± 2 °C. The procedure to apply the products via SEM analysis is described in
Appendix II.
TR-B&R007-18-A-R2, Page 12 of 42, March 13, 2018. Confidential Document.
IV – RESULTS AND DISCUSSION
From the photomicrographs obtained, image analysis was made with the Scion® for Windows software
program to detect damage to the hair fiber surface (raised cuticles, fragments) characterized by lighter colored areas.
In order to quantify the level of damage to the hair fiber surface, the “Percentage of Damage, D parameter
was determined. This parameter represents the percentage of white pixels in relation to the total number of pixels
(kept constant) of images converted into a binary white: black system – Figure 1.
Figure 1. Illustration of detection of fragments and edges via image analysis.
Virgin Hair
Appendix III summarizes the results from image analysis for each sample, out of which Graph 1 was
obtained.
Graph 1: Results of percentage damage detected on hair surface from SEM image analysis. Mean ± SD.
TR-B&R007-18-A-R2, Page 13 of 42, March 13, 2018. Confidential Document.
The results obtained for the study groups were statistically compared to Virgin group using the method
one-way ANOVA, followed by a Dunnett’s post-test, both considering a 95% confidence interval. Table 1
summarizes the results obtained. The complete data are listed in Appendix IV.
Table 1. Statistical analysis. 95% confidence interval.
Comparison Test Are there statistical difference?
Virgin vs. T01 No
Virgin vs. T02 No
Virgin vs. T03 No
Virgin vs. T07 No
Virgin vs. T08 No
Virgin vs. T09 No
Virgin vs. T13 No
Virgin vs. T14 No
Virgin vs. T15 No
Virgin vs. T19 No
Virgin vs. T20 No
Virgin vs. T21 No
Virgin vs. T22 No
Virgin vs. T27 No
Virgin vs. T28 No
Virgin vs. T29 No
Virgin vs. T30 No
According to the results obtained, the tresses submitted to the treatments T01, T02, T03, T07, T08, T09,
T13, T14, T15, T19, T20, T21, T22, T27, T28, T29 and T30 showed no statistically significant differences in values
of damage in comparison to the to the Virgin tresses (Virgin group).
The comparison between the conditions of application of the treatments was performed using the method
one-way ANOVA for repeated measures, followed by a Tukey’s post-test, considering a 95% confidence interval.
The statistical analysis is described in Appendix IV. Table 2 shows the results obtained.
Table 2. Statistical analysis. 95% of confidence interval. Comparison Test Are there statistical difference?
T01 vs. T02 No
T01 vs. T03 No
T02 vs. T03 No
T07 vs. T08 No
T07 vs. T09 No
T08 vs. T09 No
T13 vs. T14 No
T13 vs. T15 No
T14 vs. T15 No
T19 vs. T20 No
T19 vs. T21 No
T19 vs. T22 No
T20 vs. T21 No
T20 vs. T22 No
T21 vs. T22 No
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T27 vs. T28 No
T27 vs. T29 No
T27 vs. T30 No
T28 vs. T29 No
T28 vs. T30 No
T29 vs. T30 No
According to the results obtained, the tresses submitted to the treatments T01, T02 and T03 showed no
statistically significant differences in values of damage when compared to each other.
The tresses submitted to the treatments T07, T08 and T09 showed no statistically significant differences in
values of damage when compared to each other.
The tresses submitted to the treatments T19, T20, T21 and T22 showed no statistically significant
differences in values of damage when compared to each other.
The tresses submitted to the treatments T27, T28, T29 and T30 showed no statistically significant
differences in values of damage when compared to each other.
Figure 2 illustrates images of the hair fibers for the treatment groups.
Virgin
T01
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T27
T28
T29
T30
Figure 2. Examples of images for treatment groups.
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Bleached/Dyed Hair
Appendix III summarizes the results from image analysis for each sample, out of which Graph 2 was
obtained.
Graph 2: Results of percentage damage detected on hair surface from SEM image analysis. Mean ± SD.
The results obtained for the study groups were statistically compared to Bleached/Dyed group using the
method one-way ANOVA, followed by a Dunnett’s post-test, both considering a 95% confidence interval. Table 3
summarizes the results obtained. The complete data are listed in Appendix IV.
Table 3. Statistical analysis. 95% confidence interval.
Comparison Test Are there statistical difference?
Bleached/Dyed vs. T04 No
Bleached/Dyed vs. T05 No
Bleached/Dyed vs. T06 No
Bleached/Dyed vs. T10 No
Bleached/Dyed vs. T11 No
Bleached/Dyed vs. T12 No
Bleached/Dyed vs. T16 No
Bleached/Dyed vs. T17 No
Bleached/Dyed vs. T18 No
Bleached/Dyed vs. T23 Yes
Bleached/Dyed vs. T24 Yes
Bleached/Dyed vs. T25 No
Bleached/Dyed vs. T26 No
Bleached/Dyed vs. T31 Yes
Bleached/Dyed vs. T32 Yes
Bleached/Dyed vs. T33 No
Bleached/Dyed vs. T34 No
TR-B&R007-18-A-R2, Page 20 of 42, March 13, 2018. Confidential Document.
According to the results obtained, the tresses submitted to the treatments T04, T05, T06, T10, T11, T12,
T16, T17, T18, T25, T26, T33 and T34 showed no statistically significant differences in values of damage in
comparison to the to the Bleached/Dyed group.
The tresses submitted the treatments T23, T24, T31 and T32 showed significantly lower results of damage
values in comparison to the to the Bleached/Dyed group.
The comparison between the conditions of application of the treatments was performed using the method
one-way ANOVA for repeated measures, followed by a Tukey’s post-test, considering a 95% confidence interval.
The statistical analysis is described in Appendix IV. Table 4 shows the results obtained.
Table 4. Statistical analysis. 95% of confidence interval.
Comparison Test Are there statistical difference?
T04 vs. T05 No
T04 vs. T06 Yes
T05 vs. T06 No
T10 vs. T11 No
T10 vs. T12 No
T11 vs. T12 No
T16 vs. T17 No
T16 vs. T18 No
T17 vs. T18 No
T23 vs. T24 No
T23 vs. T25 Yes
T23 vs. T26 Yes
T24 vs. T25 No
T24 vs. T26 Yes
T25 vs. T26 No
T31 vs. T32 No
T31 vs. T33 No
T31 vs. T34 No
T32 vs. T33 No
T32 vs. T34 No
T33 vs. T34 No
According to the results obtained, the tresses submitted to the treatments T04 and T06 showed no
statistically significant differences in values of damage when compared to T05 group.
The tresses submitted to the treatment T04 showed significant lower values of damage when compared to
the treatment T06.
The tresses submitted to the treatments T10, T11 and T12 showed no statistically significant differences in
values of damage when compared to each other.
The tresses submitted to the treatments T23 and T24 showed no statistically significant differences in values
of damage when compared to each other.
The tresses submitted to the treatment T23 showed significant lower values of damage when compared to
the treatments T25 and T26.
The tresses submitted to the treatments T24and T26 showed no statistically significant differences in values
of damage when compared to the treatment T25.
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The tresses submitted to the treatment T24 showed significant lower values of damage when compared to
the treatment T26.
The tresses submitted to the treatments T31, T32, T33 and T34 showed no statistically significant
differences in values of damage when compared to each other.
Table 5 shows the “Reduction of Damage, RD”, in percentage and in number of times, calculated for T23,
T24, T31 and T32 treatments in comparison to Bleached/Dyed group, according to Equations 1 and 2.
CTRL
TRTCTRL
D
DDRD *100(%)
Equation 1. Reduction of Damage (%) of treatments groups relative to the CTRL group. CTRL= Bleached/Dyed
TRT
CTRL
D
DRD
Equation 2. Reduction of Damage (number of times) of treatments groups relative to the CTRL group. CTRL= Bleached/Dyed
Table 5. “Reduction of Damage, RD”of the treatment group (in percentage and number of times) compared to the
Bleached/Dyed group.
Study group % Number of times
T23 17 1.2
T24 15 1.2
T31 15 1.2
T32 14 1.2
Figure 3 illustrates images of the hair fibers for the treatment groups.
Bleached
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T34
Figure 3. Examples of images for treatment groups.
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V – CONCLUSION
In the present study, tresses of natural and bleached/dyed Caucasian hair were submitted 12 applications
of the treatments as described below:
Virgin Caucasian Hair - group Virgin;
Bleached and Dyed Hair – group Bleached/Dyed;
Virgin Hair + Monat Renew Shampoo (Normal Condition) – group T01;
Virgin Hair + Monat Renew Shampoo (Modified Condition) – group T02;
Virgin Hair + Monat Renew Shampoo (Normal Condition) + Flat Iron – group T03;
Bleached and Dyed Hair + Monat Renew Shampoo (Normal Condition) – group T04;
Bleached and Dyed Hair + Monat Renew Shampoo (Modified Condition) – group T05;
Bleached and Dyed Hair + Monat Renew Shampoo (Normal Condition) + Flat Iron – group T06;
Virgin Hair + Monat Volume Revive Shampoo (Normal Condition) – group T07;
Virgin Hair + Monat Volume Revive Shampoo (Modified Condition) – group T08;
Virgin Hair + Monat Volume Revive Shampoo (Normal Condition) + Flat Iron – group T09;
Bleached and Dyed Hair + Monat Volume Revive Shampoo (Normal Condition) – group T10;
Bleached and Dyed Hair + Monat Volume Revive Shampoo (Modified Condition) – group T11;
Bleached and Dyed Hair + Monat Volume Revive Shampoo (Normal Condition) + Flat Iron – group T12;
Virgin Hair + Monat Black Shampoo + Conditioner (Normal Condition) – group T13;
Virgin Hair + Monat Black Shampoo + Conditioner (Modified Condition) – group T14;
Virgin Hair + Monat Black Shampoo + Conditioner (Normal Condition) + Flat Iron – group T15;
Bleached and Dyed Hair + Monat Black Shampoo + Conditioner (Normal Condition) – group T16;
Bleached and Dyed Hair + Monat Black Shampoo + Conditioner (Modified Condition) – group T17;
Bleached and Dyed Hair + Monat Black Shampoo + Conditioner (Normal Condition) + Flat Iron – group
T18;
Virgin Hair + Monat Renew Shampoo + Monat Restore Leave-in Conditioner (Normal Condition) – group
T19;
Virgin Hair + Monat Renew Shampoo + Monat Restore Leave-in Conditioner (Modified Condition) – group
T20;
Virgin Hair + Monat Renew Shampoo + Monat Restore Leave-in Conditioner (Normal Condition) + Flat Iron
– group T21;
Virgin Hair + Monat Renew Shampoo + Monat Restore Leave-in Conditioner (Modified Condition) + Flat
Iron – group T22;
Bleached and Dyed Hair + Monat Renew Shampoo + Monat Restore Leave-in Conditioner (Normal Condition)
– group T23;
TR-B&R007-18-A-R2, Page 28 of 42, March 13, 2018. Confidential Document.
Bleached and Dyed Hair + Monat Renew Shampoo + Monat Restore Leave-in Conditioner (Modified
Condition) – group T24;
Bleached and Dyed Hair + Monat Renew Shampoo + Monat Restore Leave-in Conditioner (Normal Condition)
+ Flat Iron – group T25;
Bleached and Dyed Hair + Monat Renew Shampoo + Monat Restore Leave-in Conditioner (Modified
Condition) + Flat Iron – group T26;
Virgin Hair + Monat Volume Revive Shampoo + Monat Volume Revitalize Conditioner (Normal Condition) –
group T27;
Virgin Hair + Monat Volume Revive Shampoo + Monat Volume Revitalize Conditioner (Modified Condition)
– group T28;
Virgin Hair + Monat Volume Revive Shampoo + Monat Volume Revitalize Conditioner (Normal Condition) +
Flat Iron – group T29;
Virgin Hair + Monat Volume Revive Shampoo + Monat Volume Revitalize Conditioner (Modified Condition)
+ Flat Iron – group T30;
Bleached and Dyed Hair + Monat Volume Revive Shampoo + Monat Volume Revitalize Conditioner (Normal
Condition) – group T31;
Bleached and Dyed Hair + Monat Volume Revive Shampoo + Monat Volume Revitalize Conditioner (Modified
Condition) – group T32.
Bleached and Dyed Hair + Monat Volume Revive Shampoo + Monat Volume Revitalize Conditioner (Normal
Condition) + Flat Iron – group T33;
Bleached and Dyed Hair + Monat Volume Revive Shampoo + Monat Volume Revitalize Conditioner (Modified
Condition) + Flat Iron – group T34.
Virgin Hair
According to the results, the tresses submitted to 12 cycles of treatments using “Monat Renew Shampoo”,
“Monat Volume Revive Shampoo”, “Monat Black Shampoo + Conditioner”, “Monat Renew Shampoo + Monat
Restore Leave-in Conditioner” and “Monat Volume Revive Shampoo + Monat Volume Revitalize Conditioner”, on
Normal or Modified conditions showed no effect on surface damage of natural hair.
Moreover, the tresses submitted to these treatments following flat iron application showed no effect on
surface damage of natural hair, either.
Bleached/Dyed Hair
According to the results, the tresses submitted to 12 cycles of treatments using “Monat Renew Shampoo”,
“Monat Volume Revive Shampoo”, “Monat Black Shampoo + Conditioner”, “Monat Renew Shampoo + Monat
TR-B&R007-18-A-R2, Page 29 of 42, March 13, 2018. Confidential Document.
Restore Leave-in Conditioner” and “Monat Volume Revive Shampoo + Monat Volume Revitalize Conditioner”, on
Normal or Modified conditions showed no effect on surface damage of bleached and dyed hair.
Furthermore, the tresses submitted to these treatments following flat iron application showed no effect on
surface damage of bleached and dyed hair ether.
Finaly, the the products “Monat Renew Shampoo + Monat Restore Leave-in Conditioner” and “Monat
Volume Revive Shampoo + Monat Volume Revitalize Conditioner” slightly contribuite to improve smoothing of the
cuticule when compared to the bleached and dyed hair.
The table below shows the Reduction of Damage, RD, calculated to the treatments T23, T24, T31 and T32:
“Reduction of Damage, RD”of the treatment group (in percentage and number of times) compared to the Bleached/Dyed
group.
Study group % Number of times Bleached and Dyed Hair + Monat Renew Shampoo + Monat Restore Leave-in
Conditioner (Normal Condition) 17 1.2
Bleached and Dyed Hair + Monat Renew Shampoo + Monat Restore Leave-in Conditioner (Modified Condition)
15 1.2
Bleached and Dyed Hair + Monat Volume Revive Shampoo + Monat Volume Revitalize Conditioner (Normal Condition)
15 1.2
Bleached and Dyed Hair + Monat Volume Revive Shampoo + Monat Volume Revitalize Conditioner (Modified Condition)
14 1.2
TR-B&R007-18-A-R2, Page 30 of 42, March 13, 2018. Confidential Document.
This document is authorized for internal use by the sponsor, and by government supervision agencies, including ANVISA/Health Ministry for the purposes of enrolling the tested product.
Copyright © 2018 Kosmoscience. All rights reserved. No part of this publication may be reproduced, distributed, or transmitted in any means, including photocopying,
recording, or other electronic or mechanical method, without the prior written permission of the Publisher.
Administrative Head: Technical Head: Quality Head:
Adriano S. Pinheiro Chemist CRQ-IV 04245232
Viviane Albarici Chemist, PhD
Jefferson R. Bongarthner Chemist CRQ-IV 04261924
TR-B&R007-18-A-R2, Page 31 of 42, March 13, 2018. Confidential Document.
VI – REFERENCES
1. GAMEZ-GARCIA, M. Effects of some oils, emulsions, and other aqueous systems on the mechanical
properties of hair at small deformations. J. Soc. Cosmet. Chem. 44, p. 69-87 (1993).
2. CHAHAL, S. P.; JONES, R. T. Moisture regulation of hair by cosmetic proteins as demonstrated by dynamic
vapour sorption. A novel efficacy testing technique, Proc. 14th Latin American and Therlan Cong. Cosmet.
Chem. Santiago Chile. p. 45-56 (1999).
3. WILSON, T. Ed., Confocal Microscopy (Academic Press, San Diego, 1990).
4. SWIFT, J. A. “The Histology of Keratin Fibers” in the Chemistry of Natural Proteins Fibers, R. S. Asquith
(Plenum Press, New York, 1977), p. 81-146.
5. PINHEIRO, S. A. et al. Identificação e Prevenção dos Danos Oxidativos em Fibras Capilares por Marcadores
Fluorescentes. XIX Congresso Brasileiro de Cosmetologia, 2005, São Paulo. Revista Cosmetics & Toiletries.
6. KASPRZYK, E.; DELDOTTO, L. Hydrolyzed Quinoa for Hair Repair and Gloss, Cosmetics & Toiletries,
Formulation/Hair, 2013.
7. MICHAELIS: moderno dicionário da língua portuguesa. São Paulo: Companhia Melhoramentos, 1998-
(Dicionários Michaelis). 2259p.
8. JONES, R. T.; CHAHAL, S. P. The use of radiolabelling techniques to measure substantivity and penetration
into hair of protein hydrolysated, Internat. J. Cosmet. Sci. 19, p. 215-226 (1997).
9. GRUBER, J. V.; WINNIK, F. M. Cationic Polymer Deposition, J. Cosmet. Sci. 2001.
10. WEIGMANN H-D., KAMATH Y.K., REUTSCH S. B., BUSCH P. , TESMANN H. “Characterization of surface
deposits on human hair fibers”. J Soc Cosmet Chem, 41:379 (1990).
11. ANA LUÍSA SILVA SANT´ANNA, “Estudo da deposição de ceramidas sobre a fibra capilar para o combate
de danos cuticulares”. Dissertação de Mestrado. Universidade Estadual Paulista, 2000.
12. JOHNSON, D .H. “Hair and Hair Care”. Marcel Dekker, Inc. New York, (1997).
TR-B&R007-18-A-R2, Page 32 of 42, March 13, 2018. Confidential Document.
VII – HISTORY OF REVIEWS
Review Description Date
0 First issuance March 12th, 2018
1 The Sponsor identification was replaced, the code number of samples
was added on page 9 and the conclusions were re-written. March 13th, 2018
2 The identification of samples was updated on page 9 March 13th, 2018
TR-B&R007-18-A-R2, Page 33 of 42, March 13, 2018. Confidential Document.
APPENDIX I – Formulation of the samples forwarded by the Sponsor
Unreported.
TR-B&R007-18-A-R2, Page 34 of 42, March 13, 2018. Confidential Document.
APPENDIX II – Experimental Procedure
1. Preparing hair samples
Fifty four tresses were prepared from Natural Caucasian hair and Fifty four tresses were prepared from
bleached/dyed Caucasian hair, weighing 3.0 g each and 25 cm long. All tresses underwent a standard pre-cleaning
process with 10% Sodium lauryl ether sulfate (SLES) solution for 1 minute then rinsed with running water.
2. Treatments of the Tresses
2.1. Groups T01, T04, T07, T10, T13 e T16
a) Wet the tress for 20s and remove excess water.
b) Apply 0,6 mL of Shampoo and rub the tresses for 30 seconds. Leave it on hair for 3 minutes. Rinse for 30
seconds and remove excess water.
c) Repeat the steps a and b until 12 cycles.
2.2. Groups T02, T05, T08, T11, T14 e T17
a) Wet the tress for 20s and remove excess water.
b) Apply 0,3 mL of Shampoo and rub the tresses for 30 seconds. Rinse for 30 seconds and remove excess water.
Repeat the wash.
c) Repeat the steps a and b until 12 cycles.
d) Comb the tresses for 5 times to remove the knots.
2.3. Groups T03, T06, T09, T12, T15 e T18
a) Wet the tress for 20s and remove excess water.
b) Apply 0,6 mL of Shampoo and rub the tresses for 30 seconds. Leave it on hair for 3 minutes. Rinse for 30
seconds and remove excess water.
c) Apply a cycle of 10 strokes with the flat iron at approximately 230°C. Each stroke was made starting from the
hair roots to the tips for 10 seconds.
d) Repeat the steps a and c until 12 cycles.
2.4. Groups T19, T23, T27 e T31
a) Wet the tress for 20s and remove excess water.
b) Apply 0,6 mL of Shampoo and rub the tresses for 30 seconds. Leave it on hair for 3 minutes. Rinse for 30
seconds and remove excess water.
c) Apply 0.45 mL of Conditioner and rub it for 60 seconds. Rinse the tress for 30 seconds and remove excess
water.
d) Repeat the steps a, b and c until 12 cycles.
TR-B&R007-18-A-R2, Page 35 of 42, March 13, 2018. Confidential Document.
2.5. Groups T20, T24, T28 and T32
a) Wet the tress for 20s and remove excess water.
b) Apply 0,3 mL of Shampoo and rub the tresses for 30 seconds. Rinse for 30 seconds and remove excess water.
Repeat the wash.
c) Apply 0.45 mL of Conditioner and rub it for 60 seconds. Rinse the tress for 30 seconds and remove excess
water.
d) Repeat the steps a, b and c until 12 cycles.
2.6. Groups T21, T25, T29 and T33
a) Wet the tress for 20s and remove excess water.
b) Apply 0,6 mL of Shampoo and rub the tresses for 30 seconds. Leave it on hair for 3 minutes. Rinse for 30
seconds and remove excess water.
c) Apply 0.45 mL of Conditioner and rub it for 60 seconds. Rinse the tress for 30 seconds and remove excess
water.
d) Apply a cycle of 10 strokes with the flat iron at approximately 230°C. Each stroke was made starting from the
hair roots to the tips for 10 seconds.
e) Repeat the steps a, b, c and d until 12 cycles.
2.7. Groups T22, T26, T30 and T34
a) Wet the tress for 20s and remove excess water.
b) Apply 0,3 mL of Shampoo and rub the tresses for 30 seconds. Rinse for 30 seconds and remove excess water.
Repeat the wash.
c) Apply 0.45 mL of Conditioner and rub it for 60 seconds. Rinse the tress for 30 seconds and remove excess
water.
d) Apply a cycle of 10 strokes with the flat iron at approximately 230°C. Each stroke was made starting from the
hair roots to the tips for 10 seconds.
e) Repeat the steps a, b, c and d until 12 cycles.
TR-B&R007-18-A-R2, Page 36 of 42, March 13, 2018. Confidential Document.
APPENDIX III – Experimental Data Obtained
Image Analysis: Software Program: Scion® for Windows.
Values of percentage of damage obtained via Scion® for Windows software program.
Virgin T01 T02 T03 T07 T08 T09 T13 T14
20.55 21.41 20.39 22.82 20.94 19.65 22.31 20.40 21.29
18.80 20.65 21.06 19.97 17.14 20.82 19.44 19.84 16.16 18.98 17.60 19.84 21.58 20.83 19.47 19.96 18.89 18.00
20.72 21.41 20.85 21.45 20.04 20.59 20.12 18.48 20.52
18.26 20.69 20.59 21.29 19.91 18.91 19.82 17.89 20.69
T15 T19 T20 T21 T22 T27 T28 T29 T30
21.65 18.45 19.12 21.81 24.58 18.06 18.90 17.92 20.09
18.38 18.31 17.18 18.94 19.72 15.94 18.32 19.25 21.19 17.71 20.37 20.56 19.78 18.47 21.46 19.15 22.47 18.64
18.41 17.25 19.64 19.34 18.87 15.66 19.88 17.60 20.39
23.47 18.88 22.04 20.81 21.52 19.78 16.96 21.45 21.72
Bleached/Dyed T04 T05 T06 T10 T11 T12 T16 T17
22.28 23.07 22.80 24.65 24.78 21.38 21.45 21.89 21.38
24.64 20.97 22.40 23.52 20.11 20.90 23.80 23.03 24.53
25.42 21.33 23.13 24.48 22.22 23.14 22.72 22.73 21.54
23.91 23.00 21.65 24.17 20.72 23.45 24.34 22.79 23.25 26.82 23.49 23.13 23.11 23.45 23.33 22.14 21.83 23.59
T18 T23 T24 T25 T26 T31 T32 T33 T34
22.21 20.94 21.25 22.67 25.66 18.25 19.95 24.53 24.51
21.75 20.22 21.66 23.28 22.60 22.17 18.90 22.24 23.76
24.61 19.04 20.79 22.04 26.14 20.60 20.36 25.64 23.13 22.70 20.09 20.37 24.96 23.17 21.82 23.35 21.85 20.27
24.54 21.39 20.69 21.20 21.88 21.93 23.07 22.28 22.66
TR-B&R007-18-A-R2, Page 37 of 42, March 13, 2018. Confidential Document.
APPENDIX IV – Statistical Analysis
Method: One-way ANOVA with Dunnett’s post-test. Confidence Interval: 95%.
Data: Damage %, listed in Appendix III. Virgin vs Treatments
Software: GraphPad™ Prism® 6.0.
One-way analysis of variance Virgin vs Treatments
F 1.253
P value 0.2481
P value summary ns Are differences among means statistically significant? (P < 0.05) No
R square 0.2284
ANOVA table SS DF MS Treatment (between columns) 55.43 17 3.261
Residual (within columns) 187.3 72 2.601
Total 242.7 89
Dunnett's multiple comparisons test Mean Diff. 95% CI of diff. Significant?
Virgin vs. T01 -0.8900 -3.891 to 2.111 No Virgin vs. T02 -1.084 -4.085 to 1.917 No
Virgin vs. T03 -1.960 -4.961 to 1.041 No
Virgin vs. T07 -0.3100 -3.311 to 2.691 No
Virgin vs. T08 -0.4260 -3.427 to 2.575 No Virgin vs. T09 -0.8680 -3.869 to 2.133 No
Virgin vs. T13 0.3620 -2.639 to 3.363 No
Virgin vs. T14 0.1300 -2.871 to 3.131 No
Virgin vs. T15 -0.4620 -3.463 to 2.539 No
Virgin vs. T19 0.8100 -2.191 to 3.811 No Virgin vs. T20 -0.2460 -3.247 to 2.755 No
Virgin vs. T21 -0.6740 -3.675 to 2.327 No
Virgin vs. T22 -1.170 -4.171 to 1.831 No
Virgin vs. T27 1.282 -1.719 to 4.283 No Virgin vs. T28 0.8200 -2.181 to 3.821 No
Virgin vs. T29 -0.2760 -3.277 to 2.725 No
Virgin vs. T30 -0.9440 -3.945 to 2.057 No
TR-B&R007-18-A-R2, Page 38 of 42, March 13, 2018. Confidential Document.
Method: One-way ANOVA with Tukey’s post-test. Confidence Interval: 95%.
Data: Damage %, listed in Appendix III. Comparison Between condition of application on Virgin hair.
Software: GraphPad™ Prism® 6.0.
One-way analysis of variance
F 1.300
P value 0.3084
P value summary ns
Are differences among means statistically significant? (P < 0.05)
No
R square 0.1781
ANOVA table SS DF MS Treatment (between columns) 3.250 2 1.625
Residual (within columns) 15.00 12 1.250
Total 18.25 14
Tukey's multiple comparisons test Mean Diff. 95% CI of diff. Significant?
T01 vs. T02 -0.1940 -2.081 to 1.693 No T01 vs. T03 -1.070 -2.957 to 0.8166 No
T02 vs. T03 -0.8760 -2.763 to 1.011 No
One-way analysis of variance
F 17.19
P value < 0.0001 P value summary ****
Are differences among means statistically significant? (P < 0.05) Yes
R square 0.1895
ANOVA table SS DF MS
Treatment (between columns) 439.2 2 219.6 Residual (within columns) 1878 147 12.78
Total 2317 149
Tukey's multiple comparisons test Mean Diff. 95% CI of diff. Significant?
T07 vs. T08 -0.8204 -2.513 to 0.8722 No T07 vs. T09 3.149 1.457 to 4.842 Yes
T08 vs. T09 3.97 2.277 to 5.662 Yes
One-way analysis of variance
F 0.2248
P value 0.8020
P value summary ns Are differences among means statistically significant? (P < 0.05)
No
R square 0.03611
ANOVA table SS DF MS Treatment (between columns) 1.805 2 0.9027
Residual (within columns) 48.19 12 4.016
Total 49.99 14
Tukey's multiple comparisons test Mean Diff. 95% CI of diff. Significant? T13 vs. T14 -0.2320 -3.613 to 3.149 No
T13 vs. T15 -0.8240 -4.205 to 2.557 No
T14 vs. T15 -0.5920 -3.973 to 2.789 No
TR-B&R007-18-A-R2, Page 39 of 42, March 13, 2018. Confidential Document.
One-way analysis of variance
F 1.172 P value 0.3513
P value summary ns
Are differences among means statistically significant? (P < 0.05)
No
R square 0.1802
ANOVA table SS DF MS
Treatment (between columns) 10.65 3 3.550
Residual (within columns) 48.47 16 3.029
Total 59.12 19 Tukey's multiple comparisons test Mean Diff. 95% CI of diff. Significant?
T19 vs. T20 -1.056 -4.205 to 2.093 No
T19 vs. T21 -1.484 -4.633 to 1.665 No
T19 vs. T22 -1.980 -5.129 to 1.169 No T20 vs. T21 -0.4280 -3.577 to 2.721 No
T20 vs. T22 -0.9240 -4.073 to 2.225 No
T21 vs. T22 -0.4960 -3.645 to 2.653 No
One-way analysis of variance
F 1.538
P value 0.2432 P value summary ns
Are differences among means statistically significant? (P < 0.05) No R square 0.2238
ANOVA table SS DF MS
Treatment (between columns) 15.44 3 5.148
Residual (within columns) 53.56 16 3.348
Total 69.00 19 Tukey's multiple comparisons test Mean Diff. 95% CI of diff. Significant?
T27 vs. T28 -0.4620 -3.773 to 2.849 No
T27 vs. T29 -1.558 -4.869 to 1.753 No
T27 vs. T30 -2.226 -5.537 to 1.085 No
T28 vs. T29 -1.096 -4.407 to 2.215 No T28 vs. T30 -1.764 -5.075 to 1.547 No
T29 vs. T30 -0.6680 -3.979 to 2.643 No
TR-B&R007-18-A-R2, Page 40 of 42, March 13, 2018. Confidential Document.
Method: One-way ANOVA with Dunnett’s post-test. Confidence Interval: 95%.
Data: Damage %, listed in Appendix III. Bleached/Dyed vs. treatments
Software: GraphPad™ Prism® 6.0.
One-way analysis of variance Virgin vs Treatments
F 3.359
P value 0.0002
P value summary ***
Are differences among means statistically significant? (P < 0.05) Yes
R square 0.4423
ANOVA table SS DF MS
Treatment (between columns) 108.2 17 6.362 Residual (within columns) 136.4 72 1.894
Total 244.5 89
Dunnett's multiple comparisons test Mean Diff. 95% CI of diff. Significant?
Bleached/Dyed vs. T04 -0.8900 -3.891 to 2.111 No
Bleached/Dyed vs. T05 -1.084 -4.085 to 1.917 No Bleached/Dyed vs. T06 -1.960 -4.961 to 1.041 No
Bleached/Dyed vs. T10 -0.3100 -3.311 to 2.691 No
Bleached/Dyed vs. T11 -0.4260 -3.427 to 2.575 No
Bleached/Dyed vs. T12 -0.8680 -3.869 to 2.133 No Bleached/Dyed vs. T16 0.3620 -2.639 to 3.363 No
Bleached/Dyed vs. T17 0.1300 -2.871 to 3.131 No
Bleached/Dyed vs. T18 -0.4620 -3.463 to 2.539 No
Bleached/Dyed vs. T23 0.8100 -2.191 to 3.811 No
Bleached/Dyed vs. T24 -0.2460 -3.247 to 2.755 No Bleached/Dyed vs. T25 -0.6740 -3.675 to 2.327 No
Bleached/Dyed vs. T26 -1.170 -4.171 to 1.831 No
Bleached/Dyed vs. T31 1.282 -1.719 to 4.283 No
Bleached/Dyed vs. T32 0.8200 -2.181 to 3.821 No Bleached/Dyed vs. T33 -0.2760 -3.277 to 2.725 No
Bleached/Dyed vs. T34 -0.9440 -3.945 to 2.057 No
Method: One-way ANOVA with Tukey’s post-test. Confidence Interval: 95%.
Data: Damage %, listed in Appendix III. Comparison Between condition of application on Bleached/Dyed vs. hair.
Software: GraphPad™ Prism® 6.0.
One-way analysis of variance
F 5.373
P value 0.0216
P value summary *
Are differences among means statistically significant? (P < 0.05)
Yes
R square 0.4724
ANOVA table SS DF MS
Treatment (between columns) 7.547 2 3.773 Residual (within columns) 8.428 12 0.7023
Total 15.97 14
Tukey's multiple comparisons test Mean Diff. 95% CI of diff. Significant?
T04 vs. T05 -0.2500 -1.664 to 1.164 No T04 vs. T06 -1.614 -3.028 to -0.1999 Yes
T05 vs. T06 -1.364 -2.778 to 0.05006 No
TR-B&R007-18-A-R2, Page 41 of 42, March 13, 2018. Confidential Document.
One-way analysis of variance
F 0.2440 P value 0.7872
P value summary ns
Are differences among means statistically significant? (P < 0.05) No R square 0.03908
ANOVA table SS DF MS
Treatment (between columns) 1.064 2 0.5319
Residual (within columns) 26.16 12 2.180
Total 27.22 14 Tukey's multiple comparisons test Mean Diff. 95% CI of diff. Significant?
T10 vs. T11 -0.1840 -2.675 to 2.307 No
T10 vs. T12 -0.6340 -3.125 to 1.857 No
T11 vs. T12 -0.4500 -2.941 to 2.041 No
One-way analysis of variance
F 0.4807
P value 0.6297
P value summary ns
Are differences among means statistically significant? (P < 0.05)
No
R square 0.07418
ANOVA table SS DF MS
Treatment (between columns) 1.261 2 0.6307 Residual (within columns) 15.74 12 1.312
Total 17.01 14
Tukey's multiple comparisons test Mean Diff. 95% CI of diff. Significant?
T16 vs. T17 -0.4040 -2.337 to 1.529 No
T16 vs. T18 -0.7080 -2.641 to 1.225 No T17 vs. T18 -0.3040 -2.237 to 1.629 No
One-way analysis of variance
F 8.113
P value 0.0016
P value summary ** Are differences among means statistically significant? (P < 0.05)
Yes
R square 0.6034
ANOVA table SS DF MS Treatment (between columns) 40.64 3 13.55
Residual (within columns) 26.72 16 1.670
Total 67.36 19
Tukey's multiple comparisons test Mean Diff. 95% CI of diff. Significant?
T23 vs. T24 -0.6160 -2.954 to 1.722 No T23 vs. T25 -2.494 -4.832 to -0.1558 Yes
T23 vs. T26 -3.554 -5.892 to -1.216 Yes
T24 vs. T25 -1.878 -4.216 to 0.4602 No
T24 vs. T26 -2.938 -5.276 to -0.5998 Yes T25 vs. T26 -1.060 -3.398 to 1.278 No
TR-B&R007-18-A-R2, Page 42 of 42, March 13, 2018. Confidential Document.
One-way analysis of variance
F 2.397
P value 0.1062
P value summary ns Are differences among means statistically significant? (P < 0.05) No
R square 0.3100
ANOVA table SS DF MS Treatment (between columns) 21.51 3 7.171
Residual (within columns) 47.88 16 2.992
Total 69.39 19
Tukey's multiple comparisons test Mean Diff. 95% CI of diff. Significant?
T31 vs. T32 -0.1720 -3.302 to 2.958 No T31 vs. T33 -2.354 -5.484 to 0.7760 No
T31 vs. T34 -1.912 -5.042 to 1.218 No
T32 vs. T33 -2.182 -5.312 to 0.9480 No
T32 vs. T34 -1.740 -4.870 to 1.390 No T33 vs. T34 0.4420 -2.688 to 3.572 No