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C H A N G E S E R V I C E R E Q U E S T E D

© 2019 Santa Cruz Biotechnology, Inc. Santa Cruz Biotechnology, the Santa Cruz Biotechnology, Inc. logo, ChemCruz, ImmunoCruz, and UltraCruz are registered trademarks of Santa Cruz Biotechnology, Inc. All rights reserved.

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ImmunoCruz® Antibody Conjugates

OVER 8,300

MONOCLONAL

PRIMARY ANTIBODIES

AVAILABLE IN 10

CONJUGATE FORMS

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Our extensive line of support products complement our proteomics and biomedical research reagents. Our support products are optimized for use in conjunction with Santa Cruz Biotechnology primary antibodies, si/shRNA and CRISPR Products and include:

SUPPORT PRODUC TS F E AT U R I N G: U LT R AC R U Z ® B LO C K I N G R E AG E N TOffering our new UltraCruz® Blocking Reagent (sc-516214) for use in

immunohistochemistry, immunofluorescence and Western blotting research

applications to minimize non-specific binding. UltraCruz® Blocking Reagent is an

animal-free proprietary solution that is a superior alternative to conventional

blocking sera, BSA or non-fat dry milk.

β-catenin (E-5): sc-7963

EWS (C-9): sc-48404

PCNA (PC10) Alexa Fluor® 488: sc-56 AF488

β-Actin (C4): sc-47778

90 K –

55 K –

43 K –

34 K –

β Actin

A B C

Near-infrared WB with β-Actin (C4): sc-47778 antibody. Blots blocked with UltraCruz® Blocking Reagent: sc-516214.

β Actin

109 K –87 K –

51 K –

35 K –

23 K –18 K –

n Blocking Reagents for Western Blotting Applications

n Protein A, G-Plus & L Agarose IP Reagents

n Western Blotting Chemiluminescence Luminol Reagent

n RIPA Lysis Buffer

n Staining Kits

n Molecular Weight Standards

n Biologically Active & Purified Proteins

n Tissue Extracts & Cell Lysates

n Transfected Cell Lysates

Cruz Marker™ Molecular Weight Standards appear only on the final Western blot image after incubation with Cruz Marker™ MW Tag Antibodies listed on the right, Cruz Marker™ compatible Mouse IgG Binding Proteins or Cruz Marker™ compatible Monoclonal Secondary Antibodies. These are not prestained markers. The ladder consists of six bands that appear on the final Western blot image: 132 kDa, 90 kDa, 55 kDa, 43 kDa, 34 kDa, 23 kDa. Cruz Marker™ Molecular Weight Standards are provided ready to use in SDS-PAGE loading buffer and can be loaded directly into a SDS-PAGE gel. No dilution or reconstitution is required.

PRODUCT CAT#

Cruz Marker™ Molecular Weight Standards sc-2035

Cruz Marker™ MW Tag-HRP sc-516732

Cruz Marker™ MW Tag-Alexa Fluor® 488 sc-516790

Cruz Marker™ MW Tag-Alexa Fluor® 647 sc-516791

Cruz Marker™ MW Tag-Alexa Fluor® 680 sc-516730

Cruz Marker™ MW Tag-Alexa Fluor® 790 sc-516731

Cruz Marker™ MW Tag (unconjugated) sc-516729

CRUZ MARKERS™ MOLECULAR WEIGHT STANDARDS AND DETECTION ANTIBODIES [NEW]

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132 K –90 K –

55 K –

43 K –

34 K –

23 K –

GAPDH

HSP 90α/β

A B C D

132 K –

90 K –

55 K –

43 K –

34 K –

23 K –

PKCα

A B C

GAPDH

132 K –90 K –

55 K –

43 K –

34 K –

23 K –

A B C D E F G

ENT1

A B C D E

132 K –90 K –

55 K –

43 K –

34 K –

23 K – CRU

Z M

ARKE

RS

C H E M C R U Z ® B I O C H E M I C A L SSanta Cruz Biotechnology offers over 175,000 specialty biochemicals under the ChemCruz® brand for research use. Our Protein-Interacting Inhibitors, Activators and Substrates are designed to work seamlessly with our monoclonal antibodies to study protein function. More generalized chemicals such as Prepared Buffers, Detergents and Solvents are suitable for everyday laboratory use. In-stock products are available for immediate, overnight shipping.

DAPI (4',6-Diamidino-2-phenylindole)CAS Number: 28718-90-3

Rac GAP1 expression in formalin-fixed HepG2 cells showing cytoskeletal localization and nuclear DAPI counterstain.

P R O D U C T C A S #

Benzonase® Nuclease 9025-65-4Dimethyl Sulfoxide (DMSO), cell culture reagent 67-68-5Geneticin (G418) Sulfate 108321-42-2Mafosfamide 84211-05-2Mitomycin C 50-07-7Okadaic Acid 78111-17-8Paraformaldehyde solution 4% in PBS 30525-89-4Pladienolide B 445493-23-2Polybrene® 28728-55-.74Puromycin dihydrochloride 78111-17-8

S E E A L L C H E M C R U Z® C H E M I C A L S AT

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› P R O T E I N - I N T E R A C T I N G I N H I B I TO R S , A C T I VATO R S & S U B S T R AT E S

› N U C L E O T I D E S , P E P T I D E S , P R O T E I N S & A M I N O A C I D S

› S T E M C E L L & C E L L C U LT U R E R E A G E N T S

› P R E PA R E D B U F F E R S

› A N T I M I C R O B I A L S

› S TA I N S , D Y E S , P R O B E S & L A B E L S

› C R O S S L I N K E R S

› C A R B O H Y D R AT E S & L I P I D S

› S O LV E N T S & L A B R E A G E N T S

› C H E L ATO R S

› D E T E R G E N T S

Hey, did you know that you can just buy that buffer?

POPULAR CHEMCRUZ® BIOCHEMICALS

DAB (3,3' Diaminobenzidine Tetrahydrochloride)CAS Number: 7411-49-6

Fibromodulin expression in formalin-fixed, paraffin- embedded human kidney tissue showing apical membrane and cytoplasmic staining of cells in a subset of tubules using DAB.

CRUZ MARKER™

MW Tag-Alexa Fluor® 647CRUZ MARKER™

MW Tag-HRPCRUZ MARKER™

MW Tag-Alexa Fluor® 790

CRUZ MARKER™

MW Tag-Alexa Fluor® 680CRUZ MARKER™

Molecular Weight StandardsCRUZ MARKER™

MW Tag-Alexa Fluor® 488

132 K –90 K –

55 K –

43 K –

34 K –

23 K –

Enolase

A B C D

UBF

132 K –

90 K –

55 K –

43 K –

34 K –

23 K –

A B C

Conjugates Mailer_FINAL_PressReady.indd 1 4/10/19 9:52 AM

n Over 21,000 well characterized, high quality monoclonal antibodies, including over 350 targeted to 256 phosphorylation-regulated activation sites

n Over 175,000 ChemCruz® Chemicals available for research applications

n siRNA, shRNA Plasmid and Lentiviral Particle gene silencers targeted to all (100%) known protein coding human and mouse genes

n CRISPR Plasmids, including CRISPR/Cas9 KO, HDR and Double Nickase Plasmids as well as CRISPR Activation Plasmids and Lentiviral Activation Particles for over 18,920 human and 18,347 mouse genes

n Now offering over 83,000 ImmunoCruz® Antibody Conjugates for direct Western Blotting and Staining.

LEADING WORLD WIDE SOURCE FOR RESEARCH PRODUCTS

Over 83,000 ImmunoCruz® Antibody Conjugates

available (8,300 monoclonal primary antibodies

in 10 popular conjugate forms) for direct

detection and use, eliminating the need for

secondary reagents. Our new Alexa Fluor® Direct

Conjugates allow for simultaneous detection

of multiple target proteins in one experiment,

producing signals with better dynamic range

and more stability than conventional enzymatic

detection methods.

IMMUNOCRUZ® ANTIBODY CONJUGATES [NEW]FOR DIRECT WESTERN BLOTS OR STAINING

E L I M I N A T E S T H E N E E D F O R S E C O N D A R Y A N T I B O D I E S

Alexa Fluor® is a trademark of Molecular Probes, Inc.

n

n

n

n

n

n

n

n

n

n

Alexa Fluor® 488 ›› 525 nm Emission

Alexa Fluor® 546 ›› 573 nm Emission

Alexa Fluor® 594 ›› 617 nm Emission

Alexa Fluor® 680 ›› 702 nm Emission

Alexa Fluor® 790 ›› 814 nm Emission

FITC (Fluorescein) ›› 525 nm Emission

PE (Phycoerytherin) ›› 578 nm Emission

Horseradish Peroxidase (HRP)

Agarose Conjugate (AC)

VIS

IBL

E L

IGH

TIN

FR

AR

ED

P O P U L A R C O N J U G A T E S10

400 nm

450 nm

500 nm

600 nm

550 nm

650 nm

700 nm1.4 μm

3 μm

15 μm

1 mm

750 nm

800 nm

850 nm

ALEXA FLUOR® DIREC T CONJUGATES FOR RGB FLUORESCENT IMAGING SYSTEMS

Dyes in the Visible Light Spectrum are suitable for use with imaging systems like iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems.

n Alexa Fluor® 488 | 525 nm Emission n Alexa Fluor® 546 | 573 nm Emission n Alexa Fluor® 594 | 617 nm Emission n Alexa Fluor® 647 | 665 nm Emission

ALEXA FLUOR® DIREC T CONJUGATES FOR NEAR-INFRARED (NIR) DETEC TION SYSTEMS

Dyes in the Near-Infrared Spectrum are suitable for use with detection systems like LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems.

PE & FITC DIREC T CONJUGATESFluorescein (FITC) and Phycoerythrin (PE) antibody conjugates for direct immunofluorescent detection.

n FITC (Fluorescein) | 525 nm Emission n PE (Phycoerythrin) | 578 nm Emission

HRP DIREC T CONJUGATESHorseradish Peroxidase (HRP) Antibody Conjugates for direct chemiluminescence (ECL) WB and chromogenic (DAB) IHC.

n Horseradish Peroxidase (HRP)

AGAROSE DIREC T CONJUGATESAgarose Antibody Conjugates (AC) for target protein enrichment by immunoprecipitation (IP).

n Agarose Conjugate (AC)

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Pol II (CTD4H8) HRP: sc-47701 HRP

PCNA (PC10) HRP: sc-56 HRP

ALDH1/2 (H-8) HRP: sc-166362 HRPHSP 27 (F-4): sc-13132 HRP

Cytokeratin 8 (C51) FITC: sc-8020 FITC

SSEA-4 (813-70) FITC: sc-21704 FITC

hnRNP A1 (4B10) FITC: sc-32301 FITC

Vimentin (E-5) PE: sc-373717 PE

pan-Cytokeratin (C11) PE: sc-8018 PE

Lamin A/C (636) PE: sc-7292 PE

Bcl-2 (100) Alexa Fluor® 488: sc-509 AF488

TCP-1 α (B-3) Alexa Fluor® 680: sc-374088 AF680 and β-Actin (C4) Alexa Fluor 790: sc-47778 AF790

GAPDH (G-9) Alexa Fluor® 680: sc-365062 AF680

p53 (DO-2) Alexa Fluor 680: sc-53394 AF680 and GAPDH (G-9) Alexa Fluor 790: sc-365062 AF790

PKC α (H-7) Alexa Fluor® 790: sc-8393 AF790 and GAPDH (G-9) Alexa Fluor 680: sc-365062 AF680

M O N O C L O N A L A N T I B O D I E S | s i R N A /s h R N A | C R I S P R | R E S E A R C H B I O C H E M I C A L S

n Broad range of proteomics research support products, including secondary antibodies, Protein A/G PLUS immunoprecipitation reagents, MW markers, cDNA transfected lysates, etc.

R E C E I V E › › 1 5 C R U Z C R E D I T S ™ F O R W R I T I N G A N O N L I N E R E V I E W AT S C B T. C O M

WB (RGB)IF

IHCFCM

WB (NIR)IF

IHCFCM

IF IHCFCM

WB (ECL)IHC

ELISA

RACK1 (B-3) HRP: sc-17754 HRP

Alexa Fluor® 647 ›› 665 nm Emission

HCAM (BU52) FITC: sc-65265 FITC

CD47 (B6H12) PE: sc-12730 PE

CD81 (1.3.3.22) FITC: sc-7637 FITC

C R U Z M A R K E R ™ M O L E C U L A R W E I G H T S TA N DA R D S A N D C R U Z M A R K E R ™ M W TAG A N T I B O D I E S AVA I L A B L E .

β Tubulin (D-10) HRP: sc-5274 HRP

Direct western blot analysis of Histone H1 expression in A-431 whole cell lysate (A) and A-431 whole cell lysate immunoprecipitated with Histone H1 (H-2) AC: sc-393358 AC (B). Detected with Histone H1 (H-2) HRP: sc-393358 HRP.

Direct near-infrared western blot analysis of CD98 expression in HeLa whole cell lysate (A) and HeLa whole cell lysate immunoprecipitated with CD98 (E-5) AC: sc-376815 AC (B). Detected with CD98 (4F2) Alexa Fluor® 680: sc-59145 AF680. Blocked with UltraCruz® Blocking Reagent: sc-516214.

Direct near-infrared western blot analysis of EGFR expression in A-431 whole cell lysate (A) and A-431 whole cell lysate immunoprecipitated with EGFR (A-10) AC: sc-373746 AC (B). Detected with EGFR (A-10) Alexa Fluor® 790: sc-373746 AF790. Blocked with UltraCruz® Blocking Reagent: sc-516214.

IP

› UltraCruz® Blocking Reagent (sc-516214) is the preferred blocking reagent for NIR-WB.

› UltraCruz® Blocking Reagent (sc-516214) is the preferred blocking reagent for IF and RGB Fluorescent WB.› UltraCruz® Blocking Reagent (sc-516214) is the preferred blocking reagent for IHC(P). Blotto is the preferred blocking reagent for ECL WB.

n Alexa Fluor® 680 | 702 nm Emission n Alexa Fluor® 790 | 814 nm Emission

Cytokeratin 19 (A-3) Alexa Fluor® 647: sc-376126 AF647

SYP (SVP38) Alexa Fluor® 647: sc-12737 AF647

A B

SYP

132 K –90 K –

55 K –

43 K –

34 K –

23 K –

A B C D

132 K –90 K –

55 K –

43 K –

Lamin A/C

Lamin A/C (636) Alexa Fluor® 594: sc-7292 AF594 A B C

Calibindin D28K

43 K –

34 K –

23 K –

Calbindin D28K (D-4) Alexa Fluor® 546: sc-365360 AF546

IP3R-II (A-5) Alexa Fluor® 594: sc-398434 AF594

Vimentin (RV202) Alexa Fluor® 488: sc-32322 AF488

A B C

Vimentin

132 K –90 K –

55 K –

43 K –

34 K –

23 K –

Cruz®

a n t i b o d y p r o d u c t s & r e a g e n t s

Immuno…leader in innovation and quality since 1991

S A N TA C R U Z B I O T E C H N O L O G Y, I N C .

CD45 (YAML 501.4) Alexa Fluor® 647: sc-65344 AF647

CD81 (5A6) Alexa Fluor® 488: sc-23962 AF488

GFRα-1 (E-11) Alexa Fluor® 546: sc-271546 AF546

Conjugates Mailer_FINAL_PressReady.indd 2 4/10/19 9:52 AM