2016 GEM Midwest Trial Results & New 2017 Releases

D. Peters, C. Gardner, M. Peters, M. ShenUSDA-ARS GEMDecember 7, 2016

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GEM IT & YT Statistics Report

Dr. Dave Peters and Dr. Mack Shen

USDA-ARS NCRPIS GEM- December 7, 2016 -

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GEM-Ames Website Usage

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Information request form (raw data)

Plant and harvest datesLocation: county, statePlot size (width x length – button-to-button)Desired stand countPlot condition (optional, but preferred)

EXPT Entry Pedigree Plot Rep WT Yield MOIST TWT PLTHT EARHT STAND SKLDG RTLDG DEARS

Commercial Checks 2016

Hybrid Midwest RM (in our 2015 tests)

MBS9508/MBS2655 ᷉109MBS3520/MBS8814 ᷉111Pioneer 34R65 ᷉109Pioneer 33F85 ᷉110Pioneer 31N27 ᷉111

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Pioneer 31D58 ᷉115

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GEM-Ames trial summary

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Total yield trial plots by year

GEM-Ames trial summary

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The Percentage of plots by GEM & COOPs

Series2 Series3

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GEM-Ames trial summary

y = 3.9504e0.0719x

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Percentage of entries out-performed check mean by year

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GEM-Ames trial summary - 2016

Summary by experiment for “quick view” on page 47 of the yield trial section

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The Number of Plots Lost Each Year

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GEM-Ames trial summary - 2016

Experiments: 44Replicated at 4 to 8 locationsPlots: 16,456The number of complete experiments lost: 0The number of total reps lost due to weather: 22 (9.0%)

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GEM-Ames trial summary - 2016

There are 98 top crosses that out-performed the mean yield of 10 checks (3.3% of 2985 entries of the top crosses)Of these 98 GEM top crosses, 32 were from the first year tests and 66 from the retestThe range of CV’s for yield was from 7.9 to 17.8% (6.2 -18.2% last year ) and the mean of the CV’s is 13.1% (last year 11.7%)

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Special Thanks to the Companies Conducted Yield Trial In-Kind Support, 2016

AgReliant Genetics, LLCBeck’s Superior Hybrids, Inc.CRD Advisors, LLCDKD GeneticsDow AgroSciences

DKD

Illinois Foundation Seeds, Inc. KMR Inc.MDSMonsanto CompanyWyffels Hybrids

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Yr 00 Yr 01 Yr 02 Yr 03 Yr 04 Yr 05 Yr 06 Yr 07 Yr 08 Yr 09 Yr 10 Yr 11 Yr 12 Yr 13 Yr 14 Yr 15

Attendants of GEM Annual Meeting

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2017 ‘Top Ten’ Trial

Purpose – to identify and test the best GEM releases from Raleigh and Ames on modern elite testers, and estimate general combining ability.

Companies agreed to devote in-kind support to topcrossing these lines on proprietary testers in winter 2016, and will test them within their own networks in 2017

Pathology Trials 2016

NLB, SLB (USDA-ARS-PSRU, Raleigh, NC)

Aflatoxin (USDA-ARS CHPRRU, MS State)

NLB and Headsmut (DuPont/Pioneer Hi-Bred)

NLB, GLS, and Goss’s Wilt (Professional Seed Research)

CRW (USDA-ARS-PGRU, Columbia, MO)

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Allelic Diversity(AD) and Double Haploids (DH) Update 2016

204 BGEM DH lines were jointly released in 2014 with Iowa State University’s Doubled Haploid (DH) Facility, representing ~52 races

22 sets of these lines have been distributed to date, plus numerous selections

278 rows of DH1 lines were increased to DH2

109 DH0 lines were increased to DH1

33 Haploid lines were treated by the ISU DH Facility for doubling

222 New BC1’s from Raleigh were selfed, and also grown in the induction iso to generate new haploids

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6,617 nursery rows and 730 isolation block rows at Ames; 5,645 nursery rows were for pollination

MBS Genetics contributed 2,516 female isolation rows

Additional breeding support from 10 other US companies

Nursery and Isolations BlocksG

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GEM Released Lines

Four GEM lines are proposed for release from the Ames program for 2017 planting

291 Releases from the Ames and Raleigh programs will be available to GEM Cooperators; 308 total released with public cooperators

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2016 GEM Releases - AmesG

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GEM Code Pedigree Race Country Type HP

GEMS-0288 (GEMS-0147/GEMS-0115)-B-002-001-001-B

GEM recycle

USA/Mexico, Thailand

25% Exotic

SS

GEMS-0289 (GEMS-0147/GEMS-0180)-B-033-001-001-B

GEM recycle

USA/Mexico, Guatemala

25% Exotic SS

GEMS-0290 BR52051:S172641-B-050-001-B Dente Amarelo Brazil

25% Exotic SS

GEMS-0291 Ki21:S(DJ7)(GEMS-0115)-B-051-002-B

Tropical Inbred x

GEM Thailand. US25%

ExoticSS

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Top Cross RM & Ear Height EstimatesAverage

GEM Code PedigreeEstimated

RMEar Height

(cm)GEMS-0288 (GEMS-0147/GEMS-0115)-B-002-001-001-B ~111 100GEMS-0289 (GEMS-0147/GEMS-0180)-B-033-001-001-B ~110 105GEMS-0290 BR52051:S172641-B-050-001-B ~110 120GEMS-0291 Ki21:S(DJ7)(GEMS-0115)-B-051-002-B ~112 132

Hybrid ChecksMBS9508XMBS2655 ~109-110 100MBS3520/MBS8814 ~111 13034R65 ~109 9033F85 ~110 13031N127 ~111 11031D58 ~114 120

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GEMS-0288, (GEMS-0147/GEMS-0115)-B-002-001-00B

- 97, 106, and 104% of check mean yields in 3 years testing- Excellent stalks, roots, late health; husk cover just to ear tips- Large kernels, average grain quality, red cob, yellow grain- Tassel score = 7- Headsmut: 95% Not infested compared to 99% infested check line

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GEMS-0289, (GEMS-0147/GEMS-0180)-B-033-001-B

- 103, 102, and 102% of check mean yields in 3 years testing- Stalks, and roots ok in hybrid testing- Stalks and late health below average in the 2016 nursery, roots ok- Husk cover 1 ½” past ear tips- Yellow grain, red cob (photo below of a white cobbed sister line)- Tassel score = 7- Headsmut: 96% Not infested compared to 99% infested check line

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GEMS-0290, BR52051:S172641-B-050-B

- 100, 105, and 102% of check mean yields in 3 years testing- Good stalks, average roots and late health - Husk cover just past ear tips- Fat ear. yellow grain, white cob - Tassel score = 7- Headsmut: 95% Not infested compared to 99% infested check line

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GEMS-0291, Ki21:S(DJ7)(GEMS-0115)-B-051-B

- 107, 87, and 108% of check mean yields in 3 years testing- ok stalks, weak on roots, good late health, tall, hi-eared- Large, girthy, conical ear, yellow grain, red cob, excellent grain quality - Tassel score = 8- Headsmut: 95% Not infested compared to 99% infested check line

Program thoughts and goals

• Increase generation of breeding crosses, via collaboration with Raleigh and cooperators

• Increase Ames testing program and corresponding nursery capacity• Improve utilization of available analytical tools • Reduce cycle time• Seek additional winter nursery options geographically• Expand disease screening efforts, especially with international

cooperators• Longer term, explore genetic solutions to more rapidly adapt unadapted

breeding crosses

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Thank you for your past and future contributions !

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