cytomorphology of normal blood cell zhaoxindong e-mail:[email protected]
TRANSCRIPT
erythron
pronormoblast
Early normoblast:basophilic
Intermediate normoblast: polychromatic
Late normoblast:
pronormoblast Shape:round or oval
15-20um in diameter
Nucleus:
large,round or oval
reddish purple in color
granulous chromatin
Pattern with 1-2 nucleoli
Cytoplasm:
deep blue and opaque
Small amount,may show
Smalltumorlike protrusion
Early normoblast:basophilic
Shape:round or oval
10-18um in diameter
Nucleus:
large, round or oval
chromatin pattern slightly coarser ,
nucleoli indistinct
Cytoplasm:
deep blue and opaque
Small ,perinuclear pale zone is obvious
Intermediate normoblast: polychromaticShape:round or oval
8-15um in diameter
Nucleus:
smaller,round or oval
chromatin more condensed
no nucleoli
Cytoplasm:
blue-gray to pink-gray
Late normoblast:
Shape: round
7-10um in diameter
Nucleus:
pyknotic nucleus
Cytoplasm:
Pinker than previous stage
Mitoic figure
Prophase: 线团期 Metaphase: 菊花期，染色体分析的最佳时期 Anaphase: 双极期 Telophase: 双子星期
Myelocyte series
Myeloblast
Promyelocyte
Myelocyte
Metamyelocyte
Band neutrophil
Segmented neutrophil
Eosinophlic granulesthe neutrophlic granules are fine and stain a
blue-pink color with Wright stain. the neutrophlic granules are fine and stain a
blue-pink color with Wright stain. are larger than Neutrophilic granules. These round and oval-shaped granules are orange and have a
Glassy or semiopaque texture. basophilic granules have a dark blue-back color and dense appearance.
Myeloblast
This cell has an average overall diameter of 10 to 20um,the nuclear chromatin is finely reticular,with 2 to 5 light-staining nucleoli. the cytoplasm is sky blue , small in amount and lacks granules.
Promyelocyte
The cell is larger than the blast stage, with an average diameter of 12 to
The nuclear chromatin is more condensed than in the blast, and nucleoli are present. the cytoplasm is a pale grayish blue. Cytoplasm have a few azurophilic granules .
Myelocyte The cell has an
average diameter of 10 to 20um. The nucleus has a more oval appearance than in previous stages, no nucleoli, and the chromatin is much more clumped than in previous stages. Cytoplasm have a few neutrophilic granules .
METAMYLOCYTE
The cell most characteristic feature is that the nucleus begins to assume an indented or kidney-bean shape. Which will continue to elongate as the cell matures through this phase.
The chromatin continues to become more condensed or clumped.
Band granulocytes
Band granulocytes has a typical elongated nucleus.
The chromatin is very clumped.
The cytoplasm have a fine and abundant specific pink neutrophilic granules .
The segmented granulocytes
The cell has a characteristic multilobed nucleus, the separate lobes are attached to each other by a fine thread like filament.
megakaryocytic series
Megakaryoblast Promegakaryocyte Granular megakaryocyte Platelet-producing megakaryocyte Nude nucleus megakaryocyte Platelet
Megakaryoblast
The cell size can be vary from 15 um to over 50 um in diameter. it is a round cell The compact nucleus has an overall color of pink and the chromatin is a red-purple color, normally, megakaryoblasts have several nucleoli, the nucleus is surrounded by basophilic cytoplasm. cytoplasmic pseudopods are frequently seen. No granules.
Promegakaryocyte
The cell size can be vary from 30 um to over 50 um in diameter ,
the chromatin continues to darken in color with increased clumping seen in the nuclear pattern, two or more distinct nucleoli may be seen. The quantity of cytoplasm increases; the basophilic color persist .pseudopods are frequently seen. Fine azurophilic granules are most abundant adjacent to the nucleus.
Granular megakaryocyte
Megakaryocytes are the largest bone marrow cells with an average size of up to 100 um,
no nucleoli distinctive feature of the megakaryocyte is that it is multilobular, not multinucleated,
Platelet-producing megakaryocyte
on completion of maturation the fully mature lobes of the magakaryocyte shed platelets excplosively from the edges of the cytoplasm.
Nude nucleus megakaryocyte
Platelets
Platelets have an average diameter of 2 to 4 um with younger platelets being large than older ones. platelets have no nucleus, the cytoplasm is light blue with evenly disperped, fine red-purple granules.
Plasmacyte series
Plasmablast
Proplasmacyte
Plasmacyte
Plasmablast
Shape:round and elliptical 14-18um in diameterNucleus: round or oval and
eccentric. reticular chromatin , nucleoli indistinctCytoplasm: basphilic and opaque
cytoplasm.abundant and vacuolate without granules
ProplasmacyteS:round and elliptical
12-16um in diameter
N:round or oval and eccentric.
chromatin coarser and more clumped than in the plasmablast stage ,
nucleoli invisible.
C: basphilic, paranuclear clear zone may become visible, vacuolate, nongranular or a few
Plasmacyte
S:round and elliptical
12-15um in diameter
N: round or oval and eccentric with nucleoli wheel spoke chromatin pattern
C:deeply basphilic, with vacuoles or frothy,nongranular
