CYTOLOGY the structure of the CELL at light

and electron-microscopic level

Institute of Histology and EmbryologyFaculty of Medicine

Comenius University

Practical training N.2

SPECIMENS

• DNA - the nuclei of the epithelial cells,

Vagina (IH-E)

• RNA – ribosomes, rough ER, nucleolus,

Spinal cord (HE)

• Mitochondria, Kidney (Regaud)

• Glycogen, Liver of rat (PAS or PAS-H)

• Pigment granules, pigmentary epithelium of

Retina (without staining)

• Brush border, microvilli – Kidney (AP)

Specimen:

DNA - the nuclei of the

epithelial cells,

Vagina

Iron hematoxylin and Eosin

Various types of nuclei in the cells

of stratified squamous epithelium

nonkeratinized

DNA – nuclei of the epithelial cells, Vagina (IH-E)

DNA – nuclei of the epithelila cells, Vagina (IH)

Specimen:

RNA – ribosomes, rough ER,

nucleolus, Spinal cord

hematoxylin and eosin

Nissl substance (tigroid) – rER in neuron

Spinal

cord

Anterior horn

RNA – ribosomes, rough ER, nucleolus, Spinal cord (HE)

Specimen:

Demonstration of mitochondria

in the cells of proximal tubules,

Kidney

Regaud

The cell of proximal tubulein kidney,mitochondria

Mitochondria, kidney (Regaud)

Mitochondria, kidney (Regaud)

erytrocytes

mitochondria

lumen

of tubule

Mitochondria, kidney (Regaud)

Specimen:

Demonstration of glycogen

in hepatocytes, Liver

PAS–H

Glycogen

particules

in hepatocyte

at EM level

Glycogen - Liver (PAS-H)

Glycogen - Liver (PAS-H)

nucleus

Glycogen - Liver (PAS-H)

nuclei

glycogen

Glycogen - Liver (PAS-H)

glycogen

Specimen:

Demonstration of endogenic

pigments –

pigmentary epithelium of Retina,

Eye ball

without any staining

Pigmentary epithelium, retina

Unstained nucleus

Pigment granules

Specimen:

Demonstration of specialized

cellular surfaces: microvilli in the

lining epithelial cells of proximal

tubules, Kidney

Evidence of positivity (red) of alkaline

phosphatase bounded at the surface

of microvilli

Microvilli – kidney (AP)