custom peptide synthesis services
DESCRIPTION
LifeTein® is the only peptide manufacturer to offer the PeptideSynTM platform of peptide synthesis services. PeptideSynTM technology is optimized to provide a continuous flow of synthesized peptides, reducing costs while maintaining quality. PeptideSynTM has been proven to enhance the efficiency of synthesis.TRANSCRIPT
1
2
Outline
Market & Technology Trends
LifeTein Technology Portfolio
LifeTein Services
3
Synthetic Therapeutic Peptides
More than 60 synthetic therapeutic peptides under 50 amino acids in
size have reached the pharmaceutical market as APIs or generics.
4
Chemical Optimization
Strategies for Lead Peptide Optimization
Drug Discovery Today Volume 15, Numbers 1/2 January 2010
5
Technology Trends
Using Nitroalkanes as Acyl Anion
Equivalents
Microwave Synthesis
Green Chemistry for Synthesis
www.nature.com/doifinder/10.1038/nature09125
6
LifeTein Technology Portfolio
PeptideSynTM Platform
Provides high-yield
peptide synthesis
Combines SPPS and
LPPS for the highest
synthesis success rate
possible
Ensures shorter
turnaround
7
LifeTein Technology Portfolio
PeptideSynTM Ligation
Synthesizes long
peptides (up to 120
amino acids)
Ensures high yield and
short turnaround
8
LifeTein Technology Portfolio
A: H-MEDSTYYKASKGC-NH2
B: H-MEDST(PTyr)YKASKGC-NH2
C: H-PKYLQNTLKLATGMRNVPEKQTT-OH
D: H-MEDSTYYKASKGC-NH2
E: H-MEDST(PTyr)YKASKGC-NH2
F: H-PKYLQNTLKLATGMRNVPEKQTT-OH
9
LifeTein Services
Custom Peptide Synthesis Service
Custom Peptide Library Synthesis Service
Custom Polyclonal Antibody Service
Custom Monoclonal Antibody Service
10
LifeTein Services
Increases yields and reduces costs
Shortens production cycle
Combines the advantages of both solid and solution phase methods
Can scale up projects rapidly to over 100 kg
Avoids solubility problems for long segments in solution phase
Advantages of PeptideSynTM Technology
11
Peptide Synthesis Service
Peptide Length 5-120 amino acids
Peptide Scale From mg to kg
Peptide Purity Crude, desalted, >75%, >85%, >90%, >95%, 98%, and >99%
Peptide
Modifications 2-5 phosphorylation sites, methylation, acetylation, amidation, MAPs(2, 4 or 8 chains), more…
Peptide Labels FITC, Biotin, 5-FAM, Rhodamine, Dansyl, TAMRA, more…
Carrier Proteins KLH, BSA, OVA
Cyclic Peptides Amide cyclic peptide (head to tail, side chain), 1, 2, or 3 disulfide bond bridges
Analysis HPLC, MS, Amino Acid Analysis (AAA), solubility testing, microbial analysis, more…
Peptide Libraries Peptide sequence length of 5-20 amino acids, 48, 96 peptides
Others Free aliquots, spacers and linkers (Ahx, mini-PEG), more…
12
Free Aliquot Service
13
Case Studies
Cell Penetrating Peptides
Name Origin Sequence
Tat family
Tat (48-60) HIV-1 protein GRKKRRQRRRPPQQ
Oligoarginine Tat derivative Rn
Penetralia family
p-Antp Antermapedia homeodomain RQIKIWFQNRRMKWKK
plsl Igl-1 homeodomain RVIRVWFQNKRCKDKK
Chimeric CPPs
Transportan Galanin-mastoparan GWTLNSAGYLLGKINLKALAA
LAKKIL
14
Case Studies
Cell Penetrating Peptides
Attached to a CPP, therapeutic cargo is delivered to an intracellular target,
thus overcoming the entry restrictions set by the plasma membrane.
15
Case Studies
Chemically synthesized peptides have free amino and carboxyl termini.
N-terminal acetylation and C-terminal amidation reduce the overall charge of the peptide. This can
increase the stability of the peptide and its resemblance to the native protein.
N-Terminal Acetylation and C-Terminal Amidation
Both modifications are free of charge
16
Peptide Antigen Design
Continuous versus Discontinuous Epitopes?
17
Peptide Antigen Design
Sequence Length 8-20 amino acids
Peptide Purity • >75% for antibody generation,
• >90% for phosphorylated peptide antibody (recommended)
Peptide Solubility • Keep the hydrophobic amino acid content below 50%
• At least one charged residue for every five amino acids
Difficult Amino Acids
• Cys and Met can undergo rapid oxidation
• Avoid multiple Ser or Pro
• Avoid multiple or adjacent β-sheet forming amino acids: Glu, Iso, Leu, Phe, Thr, Tyr, Val
Carrier Proteins KLH, BSA, OVA
Multiple Antigenic Peptides (MAPs)
18
Custom Antibody Service
Peptide Antigen Antibody Services • Polyclonal Antibodies
• Monoclonal Antibodies
Phosphorylated-Peptide Antibody Services • Polyclonal Antibodies
• Monoclonal Antibodies
Methylated-Peptide Antibody Services • Polyclonal Antibodies
• Monoclonal Antibodies
AdjuBoosterTM Adjuvant Technology
19 More information, please visit www.lifetein.com
20
Contact Information
Let us be a partner to your growth!
LifeTein LLC
150 Maple Ave
South Plainfield, NJ 07080
USA
1-732-444-3282
1-888-791-1618 (Toll Free)
1-888-791-1618 (Fax)