Curriculum VitaeDr. Muhammad Afzal Ghauri Principal

ScientistHead Industrial Biotechnology Division,

National Institute for Biotechnology and Genetic Engineering P. O. Box 577, Jhang Road, Faisalabad,

Pakistan.Tel: (+92-41) 2550814 Res: 041-2550316; Fax: (+92-41)

2651472Electronic Mail: maghauri@nibge.org &

maghauri_bpt@yahoo.com

Date of Birth 1st July 1961Domicile Rawalpindi, PunjabNationality PakistaniMarital Status Married with two kids (one daughter and one

son)

Post-Doctorate 2002-2003, Genetic Engineering & Biotechnology, University of Leicester, U.K.Ph.D. 1991, Genetic Engineering & Biotechnology, University of Wales,

U.K. M.Sc. 1985, Zoology, University of the Punjab, Lahore, Pakistan.B.Sc. 1982, Zoology, Chemistry, Psychology, University of the Punjab, Lahore, Pakistan.

1 One year competitive Postdoctoral Fellowship at the Department of Microbiology and Immunology, University of Leicester, United Kingdom in the area of Genetic Engineering and Biotechnology. Ministry of Science and Technology, Government of Pakistan awarded this fellowship on National Merit basis in the above said area (2002-2003).

2 A competitive postgraduate scholarship was awarded by Ministry of Science and Technology leading to Ph.D. in the area of Genetic Engineering and Biotechnology (1987-1991).

3 A Roll of Honour was presented by the Gordon College Rawalpindi in acknowledgment to my outstanding performance in B.Sc. examination (1982)

4 A scholarship was awarded in Anglo-Vernacular Middle Standard Examination by Education Department, Peshawar, Pakistan (1975)

PERSONAL DATA

EDUCATION

SCHOLARSHIPS AND MERITS

MEMBERSHIP OF SCIENTIFIC SOCIETIES

CV - Dr. M. Afzal Ghauri, NIBGE, Faisalabad

1 The International Society For Extremophiles, Japan2 Life Time Member of Biotechnology Society of Pakistan (BSP-LM-04).3 Pakistan Society of Biochemistry and Molecular Biology.4 Zoological Society of Pakistan

1 Currently, I am working as Principal Scientist and Head of Industrial Biotechnology Division of National Institute for Biotechnology and Genetic Engineering (NIBGE), Faisalabad, Pakistan. I joined this institute on February 10, 1993 as Senior Scientific Officer. NIBGE is an organisation of Pakistan Atomic Energy Commission. My current areas of research interest are:

Biotechnology of Extremophiles Bacterial Biodiversity and Systematics Extremozymes Gene Mining of Extreme Environments Bioresource Development for Industry

My professional duties also include technical and administrative assistance to other research groups in Industrial Biotechnology Division viz., bioprocessing of ores and fossil fuels, industrial enzymes, biochemicals & boifuel and nanobiotechnology. Moreover,we also extend our expertise in designing of bioprocesses and consultancy services to other professional and commercial organizations/industries for quality control.

2 Expert Selection Board of Bahauddin Zakariya University, Multan for Biotechnology.

3 Member of Board of Studies of NIBGE-PIEAS for the award of M.Phil. and Ph.D. degrees.

4 One year competitive Postdoctoral Fellow at the Department of Microbiology and Immunology, University of Leicester, United Kingdom in the area of Genetic Engineering and Biotechnology. The research focus was on phylogenetic analysis of microorganisms on the basis of 16S rDNA amplification by polymerase chain reaction (PCR). Moreover, amplification and cloning of mobile genetic elements, commonly called as integrons, from various microbial groups and environments were carried out. Integrons can capture environmental gene cassettes and can be used as gene mining tools prior to gene sequence knowledge. During this period, about 60 gene sequences were deposited to GenBank, USA as public domain bioresource material.

5 Convener of Institutional Committee for Contractual Recruitment at NIBGE.

6 Member of National Curriculum Revision Committee for universities (Zoology) under Higher Education Commission.

7 Member of list of Supervisors identified by Higher Education Commission for Ph.D. programme.

8 Coordinator for the M.Phil./Ph.D. Biotechnology degree courses jointly run by NIBGE, Faisalabad and Quaid-i-Azam University, Islamabad.

2

PROFESSIONAL, ACADEMIC AND RESEARCH EXPERIENCE

CV - Dr. M. Afzal Ghauri, NIBGE, Faisalabad

9 Faculty Member of NIBGE for Postgraduate Studies in Biotechnology leading to M.Phil. and Ph.D. degrees.

10 Act as External Examiner for M.Sc. Examinations at University of the Punjab, Lahore, Pakistan.

11 Member of Panel of Paper Setters for M.Sc. Examinations at University of the Punjab, Lahore, Pakistan.

12 Member of Panel of Paper Setters for M.Sc. Examinations at University of Azad Jammu & Kashmir.

13 Act as External Examiner for M.Sc./M. Phil. Examinations at University of Agriculture, Faisalabad, Pakistan.

14 Act as External Examiner for B.Sc. (Hons)/M.Sc. Examinations at Government College University Faisalabad, Pakistan.

15 Member of Panel of Paper Setters for B.Sc. (Hons)/M.Sc. Examinations at Government College University Faisalabad, Pakistan.

16 Act as Referee for COMSATS journal “Science Vision”.

17 Faculty Member of Demonstrators at the School of Biological Sciences, University of Wales, Bangor, United Kingdom during Ph.D. studies (1987-1991).

18 Second Position in Punjab Province in the competition for the post of Lecturer in Zoology through Punjab Public Service Commission. But I could not join this post as I was heading abroad for my Ph.D. studies at University of Wales, Bangor, United Kingdom.

19 Research Scholar at Centre for Advanced Molecular Biology, University of the Punjab, Lahore, Pakistan from April, 1986 to May, 1987.

1. Nasreen Akhtar. Biodesulfurization of fossil fuels by organic sulfur metabolizing bacteria. Quaid-i-Azam University Islamabad, NIBGE Campus, Faisalabad (in progress).

2. Shazia Khaliq. Molecular studies for regulation and over expression of tylosin from

Streptomyces fradiae NRRL-2702. Quaid-i-Azam University Islamabad, NIBGE Campus, Faisalabad (in progress).

3. Sadia Ilyas. Bioleaching of low-grade sulfide ore with indigenous acidophilic mesophiles and moderate thermophiles for commercial exploitation. University of Agriculture, Faisalabad (in progress).

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THESES SUPERVISED/CO-SUPERVISEDPh. D.

CV - Dr. M. Afzal Ghauri, NIBGE, Faisalabad

4. Fozia Anjum Biomineralization of black shale using indigenous microbes. University of Agriculture, Faisalabad (in progress).

5. Noreen Azhar Studies on protease coding genes in metagenomic extracts and pure extremophilic isolates. ICGEB PhD scholar, University of Peshawar (in progress).

6. Rizwan Haider Biosolubilization and biogasification of indigenous low rank coal for its applications. HEC scholar, M.Phil. leading to PhD degree programme, Quaid-i-Azam University Islamabad, NIBGE Campus, Faisalabad (in progress).

7. Ijaz Bano Characterization of chitosan based nanocomposites for biotechnological

applications. HEC scholar, PIEAS, Islamabad, NIBGE Campus, Faisalabad (in progress).

1. Culture-dependent recovery of integron linked genes from extremophilic microorganisms. M.Phil. thesis 2004, Quaid-i-Azam University, NIBGE Campus, Faisalabad (Aamira Iqbal).

2. Microbial biodiversity of Khewra salt mine as determined by 16S rDNA analysis. M.Phil. thesis 2004, Quaid-i-Azam University, NIBGE Campus, Faisalabad (Nasreen Akhtar).

3. Diversity of integron associated gene cassettes in acidophiles determined by 59 base element primer sets. M.Phil. thesis 2005, Quaid-i-Azam University, NIBGE Campus, Faisalabad (Sabahat Latif).

4. Recovery of integrons from manmade and natural environments. M.Phil. thesis 2006, Quaid-i-Azam University, NIBGE Campus, Faisalabad (Ammara Sajjad).

5 Bioprocessing of Waziristan chalcopyrite ore using mesophilic and moderate thermophilic acidophiles. M.Phil. thesis 2006, Quaid-i-Azam University, NIBGE Campus, Faisalabad (Kokab Qayyum).

6 Effect of pH on electrochemical characterization of Tylosin-DNA interaction. M.Phil. thesis 2007, Quaid-i-Azam University, NIBGE Campus, Faisalabad (Farah Majeed).

7 Production and characterization of protease from locally isolated extremophilic Bacillus clausii (ALK-7). M.Phil. thesis 2007, Quaid-i-Azam University, NIBGE Campus, Faisalabad (Maria Sahar).

8 Optimization of process parameters for tylosin production using solid state fermentation by wild type Streptomyces fradiae NRRL–270 and its gamma irradiated mutant. M.Phil. thesis 2007, Quaid-i-Azam University, NIBGE Campus, Faisalabad (Nosheen Rasheed).

9 Study of the integron profile of some industrially important bacteria. M.Phil. thesis 2008, Quaid-i-Azam University, NIBGE Campus, Faisalabad (Muhammad Ilyas).

10 Isolation and characterization of protease gene from local alkaliphilic Bacillus clausii strain ALK-7. M.Phil. thesis 2008, Quaid-i-Azam University, NIBGE Campus, Faisalabad

4

M. Phil. Degree

CV - Dr. M. Afzal Ghauri, NIBGE, Faisalabad

(Ghulam Mustafa).

11 Studies on the production of statins by local filamentous fungi. M.Phil. thesis 2008, Quaid-i-Azam University, NIBGE Campus, Faisalabad (Mamoona Arshad).

12 Studies on genetics of metal tolerance in acidophilic bacteria. M.Phil. thesis 2009, Quaid-i-Azam University, NIBGE Campus, Faisalabad (Ijaz Bano).

Tamgha-e-Baqa

United Kingdom Ph.D. Degree Studies 04-10-1987 to 09-11-1991Portugal (From UK) To attend IBS-1991 08-09-1991 to 12-09-1991United Kingdom Postdoctoral Research 31-01-2002 to 30-01-2003Italy (From UK) To attend Extremophiles 2002 21-09-2002 to 27-09-2002Germany To attend IBS-2007 01-09-2007 to 08-09-2007

Writing Urdu poetry, reading Urdu and English literature, photography, playing table tennis and badminton.

Citations given below against each publication have been taken from following links:

ISI Web of Science (http://portal.isiknowledge.com/portal.cgi?DestApp=WOS&Func=Frame),

Science Direct (http://www.sciencedirect.com)

Google Scholar (http://scholar.google.com/scholar?q=GHAURI+MA*&ie=UTF-8&oe=UTF-8&hl=en&btnG=Search)

1 Robin, S. P., Ghauri, M. A., Jabeen, F., Riazuddin, S.* and Shams, F. A. 1987. Isolation and characterization of acidophilic bacteria from the local environment. Punjab Univ. J.

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INTRAMURAL ACTIVITIES

PUBLICATIONS

MEDALS

NATIONAL & INTERNATIONAL BOOKS/MONOGRAMS / PAPERS

FOREIGN VISITS

CV - Dr. M. Afzal Ghauri, NIBGE, Faisalabad

Zool., 2:1-9.

2 Ghauri, M. A. 1991. A study of the diversity of acidophilic bacteria. Ph.D. Thesis, University of Wales, United Kingdom. (Citations 4)

3 Ghauri, M. A. and Johnson, D. B.* 1991. Physiological diversity amongst some moderately thermophilic iron-oxidising bacteria. FEMS Microbiology Ecology 85:327-334. (Impact Factor 3.598, Citations 31)

4 Johnson, D. B.*, Ghauri, M. A. and Said, M. F. 1992. Isolation and characterization of an acidophilic, heterotrophic bacterium capable of oxidizing ferrous iron. Appl. Environ. Microbiol. 58:5,1423-1428. (Impact Factor 3.686, Citations 32)

5 Johnson, D. B.*, Ghauri, M. A. and McGinness, S. 1993. Biogeochemical cycling of iron and sulfur in leaching environments. FEMS Microbiology Reviews 11:63-70. (Impact Factor 9.783, Citations 59)

6 Ghauri, M. A.*, Iqbal, M., Anwar, A. M., Shemsi, A. M., Akhtar, K. and Khalid, A. M. 1998. Iron oxidation at varied pH values by T. ferrooxidans - a potential bacterium in bioremediation processes. Biologia 44(1&2):95-100.

7 Ghauri, M. A.*, Iqbal, M., Anwar, A. M. and Khalid, A. M. 1999. Isolation and characterisation of alkalophilic bacteria from diverse habitats of Pakistan. Resource and Environ. Biotech. 2:283-294.

8 Ghauri, M. A.*, Iqbal, M., Anwar, A. M. and Khalid, A. M. 2000. Bacterial profile of a sandstone uranium heap set for bioleaching studies. Resource and Environ. Biotech. 3:1-9.

9 Ahmad, Z., Ghauri, M. A., Anwar, M. A., Iqbal, M., Rehman, M., Akhtar, K. and Khalid, A. M.* 2002. Transformation of plasmids of Acidithiobacillus ferrooxidans encoding ampicillin resistance to Escherichia coli strain DH5- for detecting copper tolerance genes on such plasmids. Resource and Environ. Biotech. 3:185-191. (Citations 3)

10 Ghauri, M. A., Khalid, A. M., Grant, S., Heaphy, S. and Grant, W. D.* 2003. Phylogenetic analysis of different isolates of Sulfobacillus spp. isolated from uranium rich environments and recovery of genes using integron specific primers. Extremophiles 7:5, 341-345. (Impact Factor 2.000, Citations 11)

11 Rauf, S., Gooding, J. J., Akhtar, K., Ghauri, M. A., Rahman, M., Anwar, M. A. and Khalid, A. M.* 2005. Electrochemical approach of anticancer drugs-DNA interaction. Journal of Pharmaceutical and Biomedical Analysis 37(2) 205-217. (Impact Factor 2.453, Citations 66)

12 Akbar, T., Akhtar, K., Ghauri, M. A., Anwar. M. A., Rehman, M., Rehman, M., Zafar, Y. and Khalid, A. M.* 2005. Relationship among acidophilic bacteria from diverse environments as determined by randomly amplified polymorphic DNA analysis (RAPD). World J. Microbiol. Biotech. 21:645-648. (Impact Factor 1.082, Citations 0)

13 Rauf, S., Ihsan, A., Akhtar, K., Ghauri, M.A., Rahman, M., Anwar, M.A. and Khalid, A.M.* 2006. Glucose oxidase immobilization on a novel cellulose acetate-

6

CV - Dr. M. Afzal Ghauri, NIBGE, Faisalabad

polymethylmethacrylate membrane. J. Biotech. 121: 351–360 (Impact Factor 2.881, Citations 33)

14 Ghauri, M. A.*, Khalid, A. M., Grant, S., Grant, W. D., and Heaphy, S. 2006. Phylogenetic analysis of bacterial isolates from man-made high pH, high salt environments and identification of gene-cassette associated ORFs. Current Microbiology 52-6:487-492 (Impact Factor 1.330, Citations 3)

15 Ghauri, M A., Okibe, N. and Johnson, D. B.* 2007. Attachment of acidophilic bacteria to solid surfaces: the significance of species and strain variation. Hydrometallurgy 85, 2-4:72-80 (Impact Factor 2.078, Citations 18)

16 Rauf, S., Nawaz, H., Akhtar, K., Ghauri, M. A. and Khalid, A. M.* 2007. Studies on sildenafil citrate (viagra) interaction with DNA using electrochemical DNA biosensor. Biosensors & Bioelectronics 22:2471-2477 (Impact Factor 5.429, Citations 6)

17 Ilyas, S., Anwar, M. A., Niazi, S. B. and Ghauri, M. A. 2007. Bioleaching of metals from electronic scrap by moderately thermophilic acidophilic bacteria. Hydrometallurgy 88:180-188 (Impact Factor 2.078, Citations 9)

18 Ilyas, S., Anwar, M. A., Niazi, S. B., Ghauri, M. A. and Khalid, A. M. 2007. Microbial leaching of iron from pyrite by moderate thermophile chemolithotrophic bacteria. Journal of Research (Science) 18 (2):159-166. (Citations 1)

19 Munawar N., Anwar, M. A., Saleem, M., Ghauri, M. A.* and Akhtar, K. A. 2007. Biodepyritization of indigenous coal using acidophilic, mesophilic and moderately thermophilic bacteria. Pakistan Journal of Zoology, 39 (3)147-151. (Impact Factor 0.200, Citations 3)

20 Akhtar, N., Ghauri, M.A.*, Iqbal, A., Anwar, M. A. and Akhtar, K. 2008. Biodiversity and phylogenetic analysis of culturable bacteria indigenous to Khewra salt mine of Pakistan and their industrial importance. Braz. J. Microbiol. 39:143-150 (Impact Factor 0.622, Citations 2).

21 llyas, S., Anwar, M. A, Niazi, S. B., Ghauri, M. A., Ahmad, B. and Khan, K. M. 2008. Bioleaching of Pb-Zn ore by moderate thermophilic chemolithotrophic bacteria. Journal Of The Chemical Society Of Pakistan 30(1):61-68. (Impact Factor 0.190, Citations 1)

22 Zafar, M. N., Abbas, I., Nadeem R., Sheikh, M. A. and Ghauri, M. A. 2009. Removal of nickel onto alkali treated rice bran. Water Air Soil Poll. 197:361-370.(Impact Factor 1.676, Citations 0)

23 Rehman, M., Anwar, M. A., Iqbal, M., Akhtar, K., Khalid, A. M. and Ghauri, M. A.* 2009. Bioleaching of high grade Pb-Zn ore by mesophilic and moderately thermophilic iron and sulphur oxidizers. Hydrometallurgy 97:1-7. (Impact Factor 2.078, Citations 0)

24 Akhtar, K.*, Khalid, A. M., Akhtar, M. W. and Ghauri, M. A. 2009. Removal and recovery of uranium from aqueous solutions by Ca-alginate immobilized Trichoderma harzianum. Bioresource Technology 100:4551-4558 (Impact Factor 4.253, Citations 3)

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CV - Dr. M. Afzal Ghauri, NIBGE, Faisalabad

25 Ghauri, M. A.*, Anwar, M. A., Akhtar, N., Haider, R. and Tawab, A. 2009. Status of coal biotechnology in Pakistan. Advanced Materials Research 71-73:513-516.

26 Khaliq, S, Akhtar, K.*, Ghauri, M. A., Iqbal, R., Khalid, A. M. and Muddassar, M. 2009.Change in colony morphology and kinetics of tylosin production after UV and gamma irradiation mutagenesis of Streptomyces fradiae NRRL-2702. Microbiological Research 164:469-477 (Impact Factor 1.771, Citations 4)

27 Khaliq, S., Rashid, N., Akhtar, K.* and Ghauri, M. A.* 2009. Production of tylosin in solid state fermentation by Streptomyces fradiae NRRL-2702 and its gamma irradiated mutant (γ-1). Letters in Applied Microbiology 49:635-640 (Impact Factor 1.640, Citations 0)

28 Anjum, F., Bhatti, H. N.*, Ghauri, M. A. and Asi, M. R. 2009. Bioleaching of copper, cobalt and zinc from black shale by Penicillium notatum. African J. Biotechnology 8(19): 5038-5045. (Impact Factor 0.565, Citations 0)

29 Akhtar, N., Ghauri, M. A.*, Anwar, M. A. and Akhtar, K. 2009. Analysis of the dibenzothiophene metabolic pathway in a newly isolated Rhodococcus spp. FEMS Microbiology Letters 301:95-102.Impact Factor 2.199, Citations 0)

30 Ghauri, M. A.*, Grant, W. D. and Heaphy S. 2010. Metagenomic accessing of genes from environmental clone library from an oxidation tank of a waste treatment plant using 59-be conserved sequence specific primers. Pakistan Journal of Zoology, 42(1):33-36. (Impact Factor 0.200, Citations 0)

31 Anjum, F., Bhatti, H. N.* and Ghauri, M. A. 2010. Enhanced bioleaching of metals from black shale using ultrasonics. Hydrometallurgy 100:122-128 (Impact Factor 2.078, Citations 0)

32 Ilyas, S., Bhatti, H. N., Ruan, C.*, Ghauri, M. A. and Anwar, M. A. 2010. Column bioleaching of metals from electronic scrap. Hydrometallurgy 101:135-140 (Impact Factor 2.078, Citations 0)

33 Ilyas, S., Bhatti, H. N.*, Bhatti, I. A. , Sheikh, M. A., and Ghauri, M. A. 2010. Bioleaching of metal ions from low grade sulphide ore: process optimization by using orthogonal experimental array design. African J. Biotechnology 9(19): 2801-2810. (Impact Factor 0.565, Citations 0)

1 Cheema, A.M., Ghauri, M.A., Islam, A. and Ali, M. 1988. Serum protein and its fractions in dwarf goat after long term feeding of different proportion of kallar grass (Leptochloa fusca). Proc. 8th Pakistan Congr. Zool. pp. 45-52.

2 Johnson, D. B., Said, M. F., Ghauri, M. A. and McGinness, S. 1990. Isolation of novel acidophiles and their potential use in bioleaching operations. In "Biohydrometallurgy 1989" ( Salley, J., McCready, R.G.L. and Wichlacz, P.L.eds. ) pp. 403-414. (Citations 5)

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NATIONAL & INTERNATIONAL PROCEEDINGS

CV - Dr. M. Afzal Ghauri, NIBGE, Faisalabad

3 Anwar, M. A., Qamar, M. A., Akhtar, K., Ghauri, M. A. and Khalid, A. M. 1995. Microbial demineralization of coal. In: Proceedings of Fifth National Chemistry Conference (Ahmad, N., Khan, A.Y., Jaffar, M., Subhani, M.S., Khan, N. And Ansari, F.L. eds), held on Oct. 25-28, 1993 hosted by Quaid-e-Azam University, Islamabad. pp. 84-86.

13 Khalid, A. M., Ghauri, M. A., Anwar, M. A., Akhtar, K. Shemsi, A. M. Bhatti, T. M. and Iqbal, M. 1995. Role of biotechnology for minerals and fossil fuels in the sustainable development of Pakistan. In: Proceedings of International Symposium on Biotechnology for Sustainable Development (Eds. K.A. Malik, A. Nasim and A.M. Khalid), held on Dec.15-20, 1993 . pp. 309-318.

14 Ghauri, M. A., Anwar, M. A., Iqbal, M., Akhtar, K., Shemsi, A .M., Niaz, L. and Khalid, A. M. 1996. New frontiers of biotechnology for minerals and fossil fuels. In: Agricultural Biotechnology (Khan, I.A. and Malik, T.A. eds). Proceedings of First Biotechnology Symposium held at Centre for Agricultural Biochemistry and Biotechnology, University of Agriculture, Faisalabad on June 27, 1996. pp. 168-178.

6 Ghauri, M. A., Anwar, M. A., Iqbal, M., Akhtar, K., Shemsi, A. M. and Khalid, A. M. 2001. Physiological diversity amongst acidophilic bacteria isolated from geochemical extreme habitats. In: Biohorizons –Biochemistry and Biotechnology: its role and need in the 21st century (Jamil, A. and Sheikh, M. A. eds), proceedings of Ist SESCOB Conference held at University of Agriculture Faisalabad, Pakistan, Vol 2: 1-4.

7 Ghauri, M. A. 2003. Gene mining by integron capture. In "Manual on Modern Techniques in Biotechnology" ( Hafeez, F. Y., Zafar, Y, Khalid, A. M. eds. ) pp. 58-60.

8 Ghauri, M. A. 2005. Status of implementation of CBD in Pakistan: priorities, gaps and recommendations. In “Proceedings of the National Consultative Workshop on the Implementation of CBD, CMS and Ramsar Convention in Pakistan” held in Islamabad from September 22-23, 2005 (IUCN, UNEP, ME-GOP. pp 114.

9 Ghauri, M. A. and Akhtar, N. 2005. Microbial Biodiversity - Significance, Methods and Future Perspectives. In the proceedings of international conference on “Workshop on Biodiversity” held in Islamabad during December, 2005.

10 Sajjad, A., Ghauri, M. A., Akhtar, N. and Akhtar, K. 2006. Recovery of integrons from manmade and natural environments. In: Proceedings of Pakistan Congress of Zoology (Ahmad, M. and Shakoori, A.R. eds) held in Lahore during April, 2006, 26:19-28.

1 Johnson, D. B.*, Ghauri, M. A. and McGinness, S. 1991. Solid media for isolating and enumerating acidophilic bacteria. Poster presented at Biohydrometallurgy 1991, Portugal. pp. 1.5.

2 Anwar, M. A., Qamar, M. A., Ghauri, M. A., Din, M. and Khalid, A. M.* 1993. Production of environmentally safe coals through microbial treatments. Poster presented at International Symposium on Biotechnology for Sustainable Development held on Dec. 15-20, Faisalabad,

9

INTERNATIONAL POSTERS

CV - Dr. M. Afzal Ghauri, NIBGE, Faisalabad

Pakistan. pp. 150.

3 Ghauri, M. A.*, Anwar, M. A., Bhatti, T. M. and Khalid, A. M. 1993. Microbial diversity in leaching environments. Poster presented at International Symposium on Biotechnology for Sustainable Development held on Dec. 15-20, Faisalabad, Pakistan. pp. 156.

4 Khalid, A. M., Qazi, M. A., Anwar, M. A. and Ghauri, M. A.* 1993. Bioprocessing of Saindak copper ores overburden. Poster presented at International Symposium on Biotechnology for Sustainable Development held on Dec. 15-20, Faisalabad, Pakistan. pp. 158.

5 Khalid, A. M.*, Shemsi, A. M., Akhtar, K., Ghauri, M. A. and Anwar, M. A. 1993. Bioaccumulation of uranium from bioleachate by fungal biomass entrapped at commercial foam. Poster presented at International Symposium on Biotechnology for Sustainable Development held on Dec. 15-20, Faisalabad, Pakistan. pp. 159.

6 Iqbal, M., Ghauri. M. A., Rehman, M., Anwar, A. M. and Khalid, A.M.* 2000.Application of acidophilic autotrophic bacteria for the recovery of valuable metals from gold containing chalcopyrite concentrate. Poster accepted for Extremophiles 2000, The 3rd International Congress on Extremophiles held at the Technical University Hamburg-Harburg in Hamburg, Germany from September 3-7, 2000. pp 347

7 Chaudhry, R., Akhtar, K., Ghauri. M. A., Iqbal, M. Khalid, A.M.* 2000. Isolation and characterisation of cyanide metabolising bacteria to be used in the leaching of precious metals. Poster accepted for Extremophiles 2000, The 3rd International Congress on Extremophiles held at the Technical University Hamburg-Harburg in Hamburg, Germany from September 3-7, 2000. pp 348

8 Anwar, M. A., Tabassum, H., Anwar, H., Ghauri, M. A., Rehman, M., Akhtar, K. and Khalid, A. M. * 2002. Optimization of process variables for microbial depyritization of coal by acidophilic moderate thermophiles. Poster presented for Extremophiles 2002", The 4th International Congress on Extremophiles held at Naples, Italy September 22-26, 2002. p431.

9 Anwar, M. A., Ghauri, M. A., Iqbal. M., Rehman, M., Akhtar, K. and Khalid, A. M. 2002. Process upscaling for depyritization of coal using locally isolated strains of acidophilic chemolithotrophic bacteria. Poster presented for Extremophiles 2002", The 4th International Congress on Extremophiles held at Naples, Italy September 22-26, 2002. p432.

10 Rehman, M., Iqbal. M., Ghauri, M. A., Anwar, M. A., Akhtar, K. and Khalid, A. M. 2002. Bioprocessing of lead-zinc high grade ores. Poster presented at Extremophiles 2002", The 4th International Congress on Extremophiles held at Naples, Italy September 22-26, 2002. p452.

11 Rehman, M., Iqbal. M., Ghauri, M. A., Anwar, M. A., Akhtar, K. and Khalid, A. M. 2002. Microbial leaching of chalcopyrite from indigenous ores. Poster presented at Extremophiles 2002", The 4th International Congress on Extremophiles held at Naples, Italy September 22-26, 2002. p453.

12 Anwar, M. A., Saleem, M., Chaudhry, T. A., Akhtar, K., Rahman, M., Hameed, S., Ghauri, M. A. and Khalid, A. M. 2003. Bacterial adhesion to mineral surfaces during pyrite

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CV - Dr. M. Afzal Ghauri, NIBGE, Faisalabad

biooxidation. Poster presented at 7th International Conference “Trends in Biochemistry and Molecular Biology” held at Institute of Biochemistry and Biotechnology, University of the Punjab, Lahore, Pakistan. April 2-5, 2003. p48

13 Heaphy, S., Ghauri, M. A., Grant, S. and Grant, W. D. 2003. Identification of integron associated ORFs from diverse environments. Poster presented at Metagenomics 2003 Conference “From Microbial Diversity to Function“ held from 12-13 June, 2003 at Darmstadt, Germany.

14 Saleem, M., Anwar, M. A., Ghauri, M. A., Akhtar, K., Rehman, M., Hafeez, F. Y. and Khalid, A. M. 2003. Development of polyclonal antibodies against MT13 strain of Sulfobacillus thermosulfidooxidans and determination of their specificity. Poster presented at International Conference Thermophiles 2003 held from 15-19 September, 2003 at University of Exeter, United Kingdom.

15 Akhtar, N, Ghauri, M. .A., Anwar, M. .A., Akhtar, K. and Khalid, A. .M. 2004. Phylogenetic Analysis of Bacteria Isolated from Khewra Salt Mine of Pakistan. Poster presented at HALOPHILES 2004 held from 9-12 September at Ljubljana, Slovania.

16 Iqbal, A. Ghauri, M. A., Akhtar, N., Anwar, M. A., Akhtar, K. and Khalid, A. M. 2004. Recovery of integron associated gene cassettes from bacteria thriving in high salt habitats. Poster presented at 5th International Congress “Extremophiles 2004” held in Cambridge, Maryland, USA by American Society of Microbiology from 19-23 September, 2004, pp 75.

17 Anwar, M. A., Saleem, M., Ghauri, M. A., Akhtar, K. and Khalid, A. M. 2004. Serological

and molecular characterization of locally isolated acidophilic chemolithotrophic bacteria used in coal heap desulphurization. Poster presented at 5th International Congress “Extremophiles 2004” held in Cambridge, Maryland, USA by American Society of Microbiology from 19-23 September, 2004, pp 75.

18 Ghauri, M. A., Iqbal, A., Akhtar, N., Anwar, M. A., Akhtar, K. and Khalid, A. M. 2005. Phylogenetic analysis and recovery of integron associated gene cassettes from bacteria thriving in high salt habitats. Poster presented at “International Biohydrometallurgy Symposium IBS-2005 to be held from 24-29 September 2005 in Cape Town, South Africa.

19 Akhtar, N., Ghauri, M. A., Anwar, M. A., Iqbal, A., Munawar, N., Akhtar, K. and Khalid, A. M. 2006. Taxonomic Evaluation of an Organic Sulphur Metabolising Isolate from Eucalyptus Plant Roots. Poster presented at “International Extremophiles Conference 2006 held from 17-21 September 2006 in Brest, Brittany, France.

20 Qayum, K., Ghauri, M. A., Akhtar, N. and Akhtar, K. 2007. Bioleachability of chalcopyrite ore using diverse acidophiles. Poster presented at “International Biohydrometallurgy Symposium IBS-2007 held from 2-5 September 2007 in Frankfurt, Germany.

21 Anjum, F., Bhatti, H. N., Ghauri, M. A. and Asgher, M. 2009. Enhanced bioleaching of metals from black shale by Aspergillus niger using ultrasonics. Poster presented at “Taibah International Chemistry Conference 2009 held in Madina, Saudi Arabia from March 23-25, 2009.

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INTERNATIONAL ABSTRACTS

CV - Dr. M. Afzal Ghauri, NIBGE, Faisalabad

1 Anwar, A. M., Ghauri, M. A., Latif, F., Chaudhry, R., Iqbal, M., Rehman, M. and Khalid, A.M. 2000. Process upscaling for desulfurization of coal using acidophilic moderate thermophiles. Paper accepted for Extremophiles 2000, The 3rd International Congress on Extremophiles held at the Technical University Hamburg-Harburg in Hamburg, Germany from September 3-7, 2000. pp 324

2 Anwar, M. A., Saleem, M., Raouf, S., Ghauri, M. A., Tabassum, H., Akhtar, K., Rahman, M. and Khalid, A. M. 2003. Effect of cyanide ions on the oxidation of various energy sources by acidophilic chemolithotrophic bacteria. Paper presented at 7th International Conference “Trends in Biochemistry and Molecular Biology” held at Institute of Biochemistry and Biotechnology, University of the Punjab, Lahore, Pakistan. April 2-5, 2003. p91.

3 Tahira, R., Akhtar, K., Ghauri, M. A., Anwar, M. A., Rahman, M., Javed N. and Khalid, A. M. 2003. Hyperproduction of tylosin by Streptomyces fradiae through genetic manipulations. Paper presented at 7th International Conference “Trends in Biochemistry and Molecular Biology” held at Institute of Biochemistry and Biotechnology, University of the Punjab, Lahore, Pakistan. April 2-5, 2003. p55.

4 Raouf S., Akhtar K., Rahman M,.Anwar M. A., Ghauri M.A. and.Khalid A.M 2003. Current status of interaction of anticancer drugs with DNA: an electrochemical approach. Paper presented at International Conference on Current Trends in Radiopharmaceuticals to be held from October 21-23, 2003 at Islamabad.

5 Iqbal A., Akhtar, N., Ghauri, M. A., Anwar M. A., Akhtar, K. and Khalid, A. M. 2004. Physicochemical investigations of different samples collected from Khewra salt mine. Paper presented at 4th International and 14th National Chemistry Conference held from May 16-18, 2004 at Lahore, pp 167

6 Saleem, M., Anwar M. A., Ghauri, M. A., Akhtar, K., Rehman M., Hafeez, F. Y. and Khalid, A. M. 2004. Development of polyclonal antisera for the identification of locally isolated acidophilic bacteria. Paper presented at 4th International and 14th National Chemistry Conference held from May 16-18, 2004 at Lahore, pp 238

7. Raouf, S., Akhtar, K., Ghauri, M. A., Rahman, M., Anwar, M. A. and A.M. Khalid 2004. Structure characterization of cellulose acetate-polymethylmethacrylate membrane and its application to glucose oxidase immobilization . “Biosensors 2004” held in Granada Conference and Exhibition Centre, Spain, 24 - 26 May 2004.

8. Anwar, M. A., Saleem, M., Ghauri, M. A., Akhtar, N., Iqbal, A., Akhtar, K. and Khalid, A. M. 2005. Biodepyritization of high-sulfur coal by heap leaching using locally isolated strains of acidophilic chemolithotrophic bacteria. Paper has been accepted for Presentation at 1st International Conference on Environmental, Industrial and Applied Microbiology(BioMicroWorld-2005) March 15-18th 2005, Badajoz, Spain.

9. Akhtar, K., Muddassar, M., Rehman, M., Ghauri, M. A., Anwar, M. A. and Khalid, A. M. 2005. Fungal RNAi vector construction for knockdown of cahb gene in Acremonium chrysogenum fungus for hyper production of cephalosporin C. Paper has been accepted for

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CV - Dr. M. Afzal Ghauri, NIBGE, Faisalabad

Presentation at 1st International Conference on Environmental, Industrial and Applied Microbiology(BioMicroWorld-2005) March 15-18th 2005, Badajoz, Spain. Code no. 536

10. Khalid, A. M., Kalsoom, A., Ihsan, I., Nawaz, H., Anwer, M. A. and Ghauri, M. A. 2005. From nanotechnology to biotechnology: A look at the not too distant future. Oral presentation on conference on “Nano-Science and Technology in Pakistan” (June13-14, 2005) COMSATS, Islamabad. P.6

11. Ghauri, M. A., Iqbal, A., Akhtar, N., Anwar, M. A., Akhtar, K. and Khalid, A. M. 2005. Biodiversity Of Bacteria Indigenous To Khewra Salt Mine And Recovery Of Integron Associated Gene Cassettes. 18th FAOBMB International Symposium: Genomics and Proteomics in Health and Agriculture held from November 20-23, 2005, Lahore Pakistan.

12. Sajjad. A., Akhtar, N., Anwar, M. A., Akhtar, K. and Ghauri, M.A. 2006. Recovery of Integrons from Manmade and Natural Environments. Paper presented at 26 th International Pakistan Congress of Zoology held from April 4-6, 2006, University of the Punjab, Lahore, Pakistan. Abstract p. 151.

13. Munawar, N., Anwar, M. A., Saleem, M., Akhtar, K. and Ghauri, M. A. 2006 Biodepyritization of Indigenous Coal Using Axenic and Consortia of Acidophilic Mesophilic and Moderately Thermophilic Bacteria. Paper presented at 26th International Pakistan Congress of Zoology held from April 4-6, 2006, University of the Punjab, Lahore, Pakistan. Abstract p. 151.

1 Anwar, M. A., Iqbal, M., Ghauri, M. A., Akhtar, K., Shemsi, A. M. and Khalid, A. M. 1995. Microbial oxidation of pyrite by pure and mixed cultures of acidophilic bacteria. Poster presented at Third National Meeting of Pakistan Society of Biochemists held from 3-6 April, 1995. pp. 91.

2 Niaz, L., Iqbal, M., Ghauri, M.A., Anwar, M.A., Shemsi, A.M., and Khalid, A.M. 1997. Development of environmentally safe process for the recovery of zinc and lead from Duddar ore. Poster presented at First National Symposium on Biotechnology for Sustainable Development held at Government College Lahore on November 24-25.

3 Iqbal, A., Akhtar, N., Ghauri, M. A., Anwar, M. A., Akhtar, K. and Ahmad M. Khalid 2004. Physicochemical investigations of different samples collected from Khewra salt mine. Poster presented at 4th International & 14th National Chemistry Conference held at PCSIR Laboratories Complex, Lahore from 16-18 May, 2004, pp 167.

1 Khalid, a. M., Iqbal, M., Qamar, M. A., Shemsi, A. M., Anwar, M. A., Ghauri, M. A., Malik, K. A. and Lodhi, S. A. 1995. Bioleaching of low-grade Saindak copper ore. Third National Meeting of Pakistan Society of Biochemists held from 3-6 April, 1995. pp. 28.

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NATIONAL POSTERS

NATIONAL ABSTRACTS

CV - Dr. M. Afzal Ghauri, NIBGE, Faisalabad

2 Ghauri, M. A., Iqbal, M., Anwar, M. A. and Khalid, A. M. 1995. Studies on alkalophilic bacteria from uranium leaching habitats. Third National Meeting of Pakistan Society of Biochemists held from 3-6 April, 1995. pp. 60.

3 Saleem, M., Anwar, M. A., Ghauri, M. A., Akhtar, K., Hafeez, F. Y. and Khalid, A. M. 2004. Development of polyclonal antisera for the identification of locally isolated acidophilic bacteria. Paper presented at 4th International & 14th National Chemistry Conference held at PCSIR Laboratories Complex, Lahore from 16-18 May, 2004, pp 238.

4 Akhtar, K., Mudassar, M., Ghauri, M. A., Anwar, M. A. and Khalid, A. M. 2004. Development of hyper producer cephalosporin C producing strain of Acremonium chrysogenum by RNA interference technique. First National Conference on “Industrial and Environmental Biotechnology” held from 29-30 September 2004, Islamabad organized by National Commission on Biotechnology, Pakistan, pp18.

5 Anwar, M. A., Ghauri, M. A., Saleem, M., Akhtar, K. and Khalid, A. M. 2004. Biodesulphurisation of indigenous fossil fuels. First National Conference on “Industrial and Environmental Biotechnology” held from 29-30 September 2004, Islamabad organized by National Commission on Biotechnology, Pakistan, pp 34.

1 Khalid, A. M. Ghauri, M. A., Iqbal, M., Qamar, M. A., Shemsi, A. M., Anwar, M. A. and Malik, K. A. 1994. A technical report was submitted to Sanidak Metals (Pvt) Limited, Quetta, Pakistan on Microbial Leaching of Low Grade Saindak Ore.

2 Khalid, A. M., Iqbal, M., Shemsi, A. M., Ghauri, M. A., Anwar, M .A. and Malik, K. A. 1996. Microbial leaching of low grade Saindak ore. Final Report Submitted to Saindak Metals (Pvt) Ltd. Quetta.Pakistan.

3 Ghauri, M. A., Khalid, A. M. and Ali, S. 2000. A technical report on “Studies on microbial growth in water of cooling tower at KCP-II and its control”. Submitted to KCP-II, Khushab, Pakistan Atomic Energy Commission, Pakistan.

4 Khalid, A. M., Zafar, Y., Ghauri, M. A., Rehman, M. 2001. Bioprocess Technology. Chapter 5 in NIBGE Activity Report 2001. Published by National Institute for Biotechnology and Genetic Engineering, P. O. Box-577, Jhang Road,Faisalabad, Pakistan. ISBN: 969-8189-05-X, p 43-52.

5 Anwar, M. A. Ghauri M. A., Rehman, M., Akhtar, K. and Khalid, A. M. 2002. Upgradation of high sulfur Pakistani coals through biotechnological process. Final Report Submitted to TWAS, Trieste, Italy.

6 Ghauri M. A., Anwar, M. A., Rehman, M., Akhtar, K. and Khalid, A. M. 2002. Studies on biodiversity of extremophiles and their use in bioprocess technology. Final Report Submitted to TWAS, Trieste, Italy.

7 Khalid A. M., Zafar, Y., Ghauri, M. A., Idrees, M. 2003. Bioprocess Technology. Chapter 5 in NIBGE Activity Report 2001. Published by National Institute for Biotechnology and

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INDUSTRIAL/CONTRACT RESEARCH/TECHNICAL REPORTS

CV - Dr. M. Afzal Ghauri, NIBGE, Faisalabad

Genetic Engineering, P. O. Box-577, Jhang Road, Faisalabad, Pakistan. ISBN: 969-8189-08-4, p 47-60.

8 Ghauri, M. A., Anwar, M. A. and Akhtar, N. 2006. Coal biodesulphurization by heap leaching at Askari Cement Ltd., Nizampur, Nowshera (establishment of 300 tonnes coal heap). Final report submitted to Biosciences Division, PAEC Headquarters, Islamabad.

1 Anwar, M. A., Ghauri, M. A., Akhtar, N., Iqbal, A., Munawar, N., Saleem, M. and Khalid, A. M. 2006. Biodepyritization Of High Sulfur Pakistani Coal By Heap Leaching. Process: Application No. 279/2006.

1 Ghauri, M. A., Akhtar, N. and Haider, R. 2008. Coal biotechnology. An article published in DAWN newspaper in Business section on March 10, 2008 (http://www.dawn.com/2008/03/10/ebr10.htm) pp 1-3

2 Ghauri, M. A. and Haider, R. 2009. FOCUS: Embarking On An Energy Age. An article published in DAWN newspaper in Sci-Tech World section on January 10, 2009 (http://www.dawn.com/weekly/science/archive/090110/science3.htm) pp 1-2

1 Launched First Urdu poetry book “AKSE WAFA” on May 22, 2004.2 Launched Second Urdu poetry book “BISAAT” on January 26, 2008.

Sixty (60) novel genes screened from various extremophilic microorganisms and extreme environments have been deposited with GenBank, USA as public domain resource.

Impact Factor: 56.513Citations: 289

1. Title of the Project Microbial Leaching Of Low Grade Saindak OreName of P. I. Dr. Ahmad M. KhalidParticipation As a research team memberFunding Agency Government of Pakistan (Saindak Metals Limited)Allocated Amount Rs. 1.6 MillionStatus (Period) Completed (1994-1995)

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JOURNAL CITATION REPORT (JCR) IMPACT FACTOR &

COMPETITIVE RESEARCH GRANTS AWARDED BY NATIONAL/INTERNATIONAL ORGANISATIONS/AGENCIES

SUBMISSIONS OF BIORESOURCE MATERIALS TO WORLD DATABASES

LITERATURE

PATENTS

PRESS

CV - Dr. M. Afzal Ghauri, NIBGE, Faisalabad

2. Title of the project Radionuclide Immobilization by Biosorption on Microbial BiomassName of P. I. Dr. Ahmad M. KhalidParticipation As a research team memberFunding Agency International Atomic Energy AgencyAllocated Amount US $ 10,000.00Status (Period) Completed (1995-1997)

3. Title of the Project Studies on Biodiversity of Extremophiles and Their Use in Bioprocess Technology.

Name of P. I. Dr. M. Afzal Ghauri Funding Agency Third World Academy of Sciences (TWAS), Italy.Allocated Amount US $ 7000.00.Status (Period) Completed (2001-2002 for 18 months)

4. Title of the project Upgradation Of High Sulfur Pakistani Coals Through Biotechnological Process

Name of P. I. Munir A. Anwar Participation As a research team memberFunding Agency Third World Academy of Sciences (TWAS), Trieste, Italy.Allocated Amount US $ 7000Status (Period) Completed (2001-2002 for 18 months)

5. Title of the Project Bacterial Leaching Of Metals From OresName of P. I. Dr. Ahmad M. KhalidParticipation As a research team memberFunding Agency Government of Pakistan (Pak-Kazakh project-MoST)Allocated Amount Rs. ~30.0 Million.Status (Period) Completed (1998-2005)

6. Title of the Project Genetic manipulations of mutation that leads to hyperproduction of Tylosin from Streptomyces fradiae NRRl-2707 and development of solid state fermentation (SSF) process using that mutant.

Name of P. I. Dr. Kalsoom AkhtarParticipation As supervisorFunding Agency International Foundation for Science, USAAllocated Amount US$ 6500Status (Period) Completed (April 2007- September 2008)

7. Title of the Project Biodesulfurization Of Indigenous Fossil FuelsName of P. I. Dr. M. Afzal GhauriFunding Agency Government of Pakistan (MoST)Allocated Amount Rs. 28.8 Million.Status (Period) In Progress (2004-)

8. Title of the Project Gene Mining Studies on Extremophilic Microorganisms using Integron Specific Probes.

Name of P. I. Dr. M. Afzal Ghauri Funding Agency Higher Education Commission, Pakistan

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CV - Dr. M. Afzal Ghauri, NIBGE, Faisalabad

Allocated Amount Rs. 28,30,000Status (Period) In Progress (2007-)

9. Title of the Project Biotechnological applications of microorganisms in bioleaching and bioremediation of metals.

Name of P. I. Dr. M. Afzal Ghauri Funding Agency Higher Education Commission, PakistanAllocated Amount Rs. 35,98,927Status (Period) In Progress (2007-)

10. Title of the Project Development of hyper producer cephalosporin C producing strain of Acremonium chrysogenum by RNA interference technique.

Name of P. I. Dr. Kalsoom AkhtarParticipation As supervisorFunding Agency Pakistan Science FoundationAllocated Amount Rs. 9,82,000Status (Period) In Progress (2007-)

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