curriculum vitae tiemi nakamura curry, ph.d. 2058 … cv-2107.pdf · curriculum vitae tiemi...

Curriculum Vitae

TIEMI NAKAMURA CURRY, PH.D.

2058 HARBOUR DRIVE

PALMYRA, NJ 08065

(856) 786-3625

CAREER SUMMARY

Doctor of Agronomy with over 35 years of experience in cellular genetics and tissue culture of tropical,

cereal and vegetable crops and ornamental plants with extensive experience in the use of scanning and

transmission electron microscopy as well as significant teaching experience in several universities.

EDUCATION

Nagoya University, Nagoya, Japan

Ph.D., Agricultural Science, 1990, Major: Agronomy

Dissertation: “Ultrastructural Studies on In Vitro Morphogenesis of Coffee and Rice Plants”

Nagoya University, Nagoya, Japan

M.S., Agricultural Science, 1987, Major: Agronomy

Thesis: “Scanning Electron Microscope Studies on Aseptic Tissue Culture of Rice and Several Other

Plants”

Tiemi Nakamura Curry

Catholic University, Campinas, SP, Brazil

B.S., Biology, 1975

B.S., Science, 1974

PROFESSIONAL EXPERIENCE

Rutgers University, Department of Plant Biology and Pathology, New Brunswick, New Jersey.

Research Associate (7/1/2014 – Present)

--Conduct advanced tissue culture and propagation in support of Rutgers Bluegrass, Dogwood,

Hazelnut, Asparagus, Lily, Strawberry, Cranberry, Grape, Basil and Blueberry Breeding and Production

Programs.

--Develop new, value-added plant species for the small fruit and consumer bedding and pot plant

markets.

--Produce “starter plants” in quantity (100 to 1,500 units) of selected varieties and pack for delivery, as

needed.

--Research plant bioreactor systems as a means of reducing cost of lily production for

commercialization.

--Train graduate and undergraduate students in the art and science of plant tissue culture.

--Teach classes: “Plant Tissue Culture”; “Introduction to Horticulture” (Lab component)

--Photograph plants for use in research documentation, classes, and marketing materials.

Research Assistant (5/1/12 – 6/30/2014)

(SAME AS ABOVE


Laboratory/Technical, Casual Hourly Employee (7/1/2010 – 4/30/2012)

--Conduct advanced tissue culture and propagation in support of Rutgers Asparagus, Lily, Cranberry

Strawberry Breeding and Production Programs.

--Develop new, value-added plant species for the small fruit, vegetable and consumer bedding and pot

plant markets.

--Research bioreactor systems as a means of reducing cost of lily production for commercialization.

Research Assistant (7/1/2009 – 6/30/2010)

--Conduct advanced tissue culture and propagation in support of Rutgers Asparagus, Lily, and



plant markets.

--Instruct others in the latest tissue culture and plant propagation techniques.

--Photograph plants for use in research documentation and marketing materials.

Post-Doctoral Associate (2005 – 2009)

--Conduct advanced tissue culture and propagation in support of Rutgers Asparagus, Lily, and



plant markets.

--Instruct others in the latest tissue-culture and plant propagation techniques.


Rutgers Blueberry and Cranberry Research and Extension Center, Chatsworth, NJ. (2004 – 2005)

University-based research center.

Research Technician

--Performed cranberry and blueberry tissue culture and pathology, DNA fingerprinting, plant chemical

analysis and related services for growers.

PhytaCell Technology, LLC, Bordentown, New Jersey (1994 – 2004)

Custom micropropagator and perennial plug supplier.

Senior Research & Development Scientist

--Responsible for developing optimum methodology for micropropagating all plants of interest to client

nurseries, including difficult-to-work plants.

--Managed tissue culture laboratory.

--Trained others to do tissue culture.

--Established product quality and control standards.

--Developed methodology for micropropagation of various plants for commercialization including

Asparagus, Hydrangea quercifolia, Heuchera, Tiarella, Spigelia, Echinacea, Asarum, Liriope, Pinus,

Maples, Birches, and Cranberries.


Fitotek Unggul (PT), Jakarta, Indonesia (1993 – 1994)

Agribusiness company specializing in the production of true-to-type plantlets for domestic and overseas

customers.

Research Scientist

Introduced high technology to company’s R&D department. Supervised laboratory personnel.

--Responsible for design and set-up of Bioreactor micropropagation program for ginger production.

--Successfully developed methodology for pineapple micropropagation using Bioreactor technology.

--Responsible for development of new methodology for propagation of various new plants for

commercialization.

DNA Plant Technology Corporation, Cinnaminson, New Jersey (1990 – 1993)

Leading agricultural biotechnology company which uses a broad spectrum of technologies to develop

and commercialize plant-based products and environmental diagnostics for consumer and industrial

markets.

Research Scientist

Worked on the Oil Palm bioreactor micropropagation program--a joint research program between

DNAP and Kumpulan Guthrie Berhard, a leading Malaysian agricultural company.

--Successfully developed efficient methodology for oil palm micropropagation.

--Key DNAP person in the transfer of this technology to Guthrie.

--Traveled to Malaysia to assist Guthrie R&D personnel with the application of this technology

--Responsible for the investigation of new methodologies for micropropagation of ornamental palms

within a United States Agency for International Development/Agricultural Biotechnology for

Sustainable Productivity project.

--Create displays explaining the tissue culture process.


Nagoya University, Nagoya, Japan (1984 –1990)

Laboratory of Biological Resources and Environmental Sciences

Teaching Assistant

--Conducted ultrastructural research using scanning and transmission electron microscopy.

--Conducted research based on cellular genetics and tissue culture of fruits, cereals, vegetables and

tropical crops.

--Instructed students in utilization of various research methodologies in studies of plant cell, tissue

and organ culture.

Institute of Agronomy, Department of Genetics, Campinas, SP, Brazil (1980 –1984)

Research Associate

--Conducted research studies in plant cell, tissue and organ culture of coffee, bamboo, eucalyptus,

rauwolfia, stevia, ipecacuanha, jaborandi and guarana.

--Responsible for the organization and maintenance of the laboratory.

--Responsible for supervision of laboratory personnel.

Laboratory of Clinical Pathology, Campinas, SP, Brazil (1977 – 1980)

Research Associate

--Conducted analytical studies of hematology, biochemistry, serology and immunology.

School of Science and Literature, Amparo, SP, Brazil (1977– 1978)

Teacher --Taught classes in organic and inorganic chemistry.


University of Campinas, Campinas, SP, Brazil (1976-1977)

Research Associate

--Conducted research study of serological leaf scaled disease.

AFFILIATIONS

Japanese Journal of Crop Science

Journal of the International Palm Society

CONTINUING EDUCATION COURSES

1. “Application of Serology to the Study of Phytopathogens,” Graduate Course of Molecular and

Cellular Biology, Biology Institute, State University of Campinas, SP, Brazil. November 1976, 63 h

duration.

2. “Microbiology,” School of Agronomy, Sao Paulo University, Piracicaba, SP, Brazil. March to

June1977, 128 h duration.

3. “Plant Cell and Tissue Organ Culture,” Graduate Course of Plant Biology, Institute of Biology, State

University of Campinas, SP, Brazil. March to June 1982, 128 h duration.

4. “High Plant Metabolism,” Graduate Course of Plant Biology, Institute of Biology, State University of

Campinas, SP, Brazil. March to June 1982, 128 h duration.


5. “Advanced Course on in vitro Plant Propagation applied to Food Production,” Sponsored by

UNESCO-ICRO and Co-sponsored by Fundacion Gran Mariscal de Ayacucho and Centro Internacional

de Cooperacion Cientifica Simon Bolivar, Fundacion Instituto Internacional de Estudios Avanzados,

Caracas, Venezuela. April 02-13 1984, 90 h duration.

6. “Plant Genetic Engineering” with the following topics: selection technique in plant cell fusion;

preparation and culture of plant protoplast; practice in gene introduction through plant vector; plant

breeding application of transformation cell; construction of minichromosome; RNA ribosomes gene of

plant cell hybrid. Nagoya University, Japan. June 1986.

7. “Principles of Biotechnology” with the following topics: protein synthesis, DNA replication, DNA

repair, recombinant DNA technology, control of gene expression, internal membranes. Nagoya

University, Japan. September to November 1896.

8. “Ultrastructure and biochemistry of pollen, general techniques for transmission electron microscopy

of pollen, process of metabolism during pollen tube wall formation.” Nagoya University, Japan. June

1987.

9. “Medical and Biological Electron Microscopic Technology.” Saint Mariana Medical University,

Kawasaki, Japan. September 15-16, 1987, 16 h duration.

10. “Application of cryomethods in electron microscope,” with following topics: cryofixation and

cryosubstitution for routine work in transmission electron microscope, comparison between chemical-

fixation and cryofixation. Nagoya University, Japan. June 1988.

11. “Pollen protoplasts, microsporogenesis and microspore development in angiosperms,” with

following topics: fusion of plant protoplasts for production of somatic hybrid plants, protoplast

production from lily microspores and pollen. Nagoya University, Japan. June 1988.


12. “Cell Engineering in Crops,” with following topics: gene transfer by protoplast transformation,

expression of transferred genes in transgenic plants, male sterile rice produced by cell fusion: fertility

restoration by Rf1 and analysis of mtDNA. Nagoya University, Japan, June 1989.

13. “Medical and Biological Electron Microscopic Technology.” Nagoya City Medical University,

Nagoya, Japan. July 20-22, 1989, 20 h duration.

RESEARCH PAPERS PRESENTED AT SCIENTIFIC MEETINGS

1. “Titre reduction of antisera maintained in the refrigerator,” 10th Brazilian Congress of

Phytopathology, Federal University of Pernambuco, Recife, PE, Brazil, September 12-20, 1977.

2. “Xanthomonas albilineans antisera prepared by lymph node injection technique,” 10th Brazilian

Congress of Phytopathology, Federal University of Pernambuco, Recife, PE, Brazil, September 12-20,

1977.

3. “Antiserum titres to Xanthomonas albilineans during the first 15 days following a single lymph node

injection,” 10th Brazilian Congress of Phytopathology, Federal University of Pernambuco, Recife, PE,

Brazil, September 12-20, 1977.

4. “Serological tests applied to leaf scald disease of sugar cane,” 16th Congress of the International

Society of Sugar Cane Technologists, Anhembi Convention Center, Sao Paulo, SP, Brazil, September 12-

20, 1977.


5. “Pseudomonas garcae antisera prepared by lymph node injection technique, 1st Congress of Sao

Paulo Phytopathology Society, Botucatu, SP, Brazil, January 18-20, 1978.

6. “In vitro vegetative propagation of Coffea spp.,” 8th Brazilian Congress of Coffee Research, Campos

do Jordao, SP, Brazil, November 25-28, 1980.

7. “In vitro propagation of orthotropic buds of Coffea spp.,” 9th Brazilian Congress of Coffee Research,

Sao Lourenco, MG, Brazil, October 27-30, 1981.

8. “In vitro axillary bud culture of coffee,” 10th Colloquium of International Scientific Association of

Coffee, ASIC, Salvador, B.A., Brazil, October 11-14, 1982.

9. “Coffee tree micropropagation,” 10th Brazilian Congress of Coffee Research, Pocos do Caldas, MG,

Brazil, August 29-September 1, 1983.

10. “Guarana tree research developed by Agronomic Institute of Campinas,” 1st Brazilian Symposium

of Guarana, Manaus, AM, Brazil, October 24-28, 1983.

11. “Structure of rice callus tissues,” 9th Congress of the Japanese Association for Plant Tissue Culture,

Kounan University, Kobe, Japan, July 16-18, 1985.

12. “Cell differentiation in rice scutellar epithelium and callus culture under aseptic conditions, “ Japan-

Singapore Seminar on “Clonal Multiplication and Transformation of Useful Plants Through Cell and

Tissue Culture,” University of Tsukuba, Ibaraki, Japan, March 22-26, 1986.


13. “Surface ultrastructure of rice tissue culture,” 181st Congress of Japanese Crop Science Society,

Utsunomiya University, Utsunomiya, Japan, April 6-7, 1986.

14. “Surface ultrastructure of callus culture of rice (Indica type),” 182nd Congress of Japanese Crop

Science Society, Gifu University, Gifu, Japan, October 7-8, 1986.

15. “Ultrastructure of leaf and callus in coffee plant,” 184th Congress of Japanese Crop Science Society,

Okayama University, Okayama, Japan, October 14-15, 1987.

16. “Ultrastructure of coffee somatic embryo,” 1st Plant Tissue Culture Colloquium - Plant Tissue

Culture for Bioengineering, The Japanese Association for Plant Tissue Culture, Nova Hall, Tsukuba,

Japan, July 19-20, 1988.

17. “Morphological studies on callus formation and regeneration process from barley immature

embryo,” Congress of Kantou Branch, Crop Science Society, Yatabe, Tsukuba, Japan, December 2, 1988.

18. “An Ultrastructural Study of Somatic Embryogenesis in Coffea spp.“7th International Congress on

Plant Tissue and Cell Culture, RAI, Amsterdam, The Netherlands, June 24-29, 1990.


RESEARCH PUBLICATIONS

1. OLIVEIRA, A.R., A.S. COSTA and T. NAKAMURA. 1977. “Titre reduction of antisera maintained in

the refrigerator.” Fit. Bras. 2:93.

2. OLIVEIRA, A.R., T. NAKAMURA, M.H. SUGIMORI and Y. MASUDA. 1977. “Antiserum titres to

Xanthomonas albilineans, during the first 15 days following a single lymph node injection.” Fit. Bras.

2:93-94.

3. OLIVEIRA, A.R., L.H. PIN, T. NAKAMURA, M.H. SUGIMORI and Y. MASUDA. 1977.

Xanthomonas albilineans antisera prepared by lymph node injection technique. “Fit. Bras. 2:94-95.

4. OLIVEIRA, A.R., T. NAKAMURA, L.H. PIN and M.H. SUGIMORI. 1977. “Serological tests applied to

leaf scald disease of sugarcane.” Proc. Intl. Soc. Sugar Cane Techn. XVI Congress 1: 459-468.

5. SUGIMORI, M.H., A.R. OLIVEIRA, T. NAKAMURA and J. RODRIGUES NETO. 1978. “Pseudomonas

garcae antisera prepared by lymph node injection technique.” Summa Phytopathologica 4:7 and 27.

6. SONDAHL, M.R. and T. NAKAMURA. 1980. “In vitro vegetative propagation of Coffea.”

Spp. VII Brazilian Congress of Coffee Research, p. 129.


7. NAKAMURA, T. and M.R. SONDAHL. 1981. “In vitro propagation of orthotropic buds of Coffea”

Spp. IX Brazilian Congress of Coffee Research, p. 162 and 163.

8. SONDAHL, M.R. and T. NAKAMURA. 1892. “In vitro axillary bud culture of Coffee.” X Colloquium

of International Scientific Association of Coffee, p. 72.

9. OLIVEIRA, V.P., M. CARDOSO, M.A.M. BOAVENTURA, T. NAKAMURA, M.R. SONDAHL, A.L.

LOURENCAO and L.A. SAEZ. 1983. “Guarana tree research developed by Agronomic Institute.” 1st

Brazilian Symposium of Guarana.

10. NAKAMURA, T. and M.R. SONDAHL. 1983. “Coffee tree micropropagation.” X Brazilian Congress

of Coffee Research, p. 116.

11. SONDAHL, M.R., T. NAKAMURA, H.P. MEDINA-FILHO, A. CARVALHO, L.C. FAZUOLI and

W.M. COSTA. 1984. “Coffee.” In: “Handbook of Plant Cell Culture, “Vol. 3, P.V. Ammirato, D.A. Evans,

W.R. Sharp and Y. Yamada (eds.), Macmillan Publishing Co., pp. 564-590.

12. SONDAHL, M.R., T. NAKAMURA and W.R. SHARP. 1985. “Propagation of Coffee.” In: “Tissue

culture in Forestry and Agriculture.” R.R. Henke, K.W. Hughes, M.P. Constantin and A. Hollaender

(eds.), Plenum Publishing Corp., pp. 215-232.

13. NAKAMURA, T. and E. MAEDA. 1985. “Structure of rice callus tissues.” 9th Congress of the

Japanese Association for Plant Tissue Culture, p 121.

14. MAEDA, E., S.H. RADI and T. NAKAMURA. 1986. “Cell differentiation in rice scutellar epithelium

and callus culture under aseptic conditions.” Japan-Singapore Seminar on “Clonal multiplication and

transformation of useful plants through cell and tissue culture,” p. 2-3.


15. NAKAMURA, T. and E. MAEDA. 1986. “Structure of rice callus tissues.” 181st Congress of Japanese

Crop Science Society. p. 272-273.

16. NAKAMURA, T. and E. MAEDA. 1986. “Surface ultrastucture of callus culture of rice (Indica type).”

182th Congress of Japanese Crop Science. p. 195-196.

17. MAEDA, E. and T. NAKAMURA 1986. “Shoot differentiation from cultured cells and character of

surface cells.” Shyokuchoyou, Japan Plant Regulator Research Association. 20:12-16.

18. KATO, T., T. NAKAMURA, K. HATTORI and E. MAEDA. 1986. “Relationship between cold

resistance at the booting stage and callus induction of cultivars in rice anther cultures.” Japan. Jour,

Crop Science. 55:542-543.

19. NAKAMURA, T. and E. MAEDA. 1987. “Ultrastructure of leaf and callus in coffee plant.” 184th

Congress of Japanese Crop Science Society. p. 21-22.

20. NAKAMURA, T. and E. MAEDA. 1988. “Ultrastructure of coffee somatic embryo.” 1st Plant Tissue

Culture Colloquium of the Japanese Association for Plant Tissue Culture. p. 66-67.

21. SUMARDI, I., T. NAKAMURA, T. TANIGUCHI and E. MAEDA. 1988. “A light and scanning

electron microscopic study of the initiation and development of sugarcane callus.” Japan. Journ. Crop

Sci. 57:765-772.

22. MAEDA, E., S.R. RADI, T. NAKAMURA and S. YAMADA. 1988. “Cellular differentiation and

morphogenesis in plant tissue culture.” In: Cell and Tissue Culture in Field Crop Improvement. J. Bay

(ed.) Petersen FFTC Book Ser. No. 38, pp. 13-23.


23. ENOMOTO, S., T. NAKAMURA, T. KOMATSUDA, S. NAKAJIMA and E. MAEDA. 1988.

“Morphological studies on callus formation and regeneration process from barley immature embryo.”

Kantou Br. Crop Sci. Soc. Japan 3:47-48.

24. NAKAMURA, T. and E. MAEDA. 1989. “A scanning electron microscope study on Japonica type rice

callus cultures, with emphasis on plantlet initiation.” Japan, Jour, Crop Sci. 58:395-403.

25. NAKAMURA, T. and E. MAEDA. 1990. “A scanning electron microscope study on callus growth

from coffee (Coffea arabica L) leaf explants.” Japan. Jour. Crop Sci. 59:377-383.

26. NAKAMURA, T. and E. MAEDA. 1990. “An ultrastructural study of somatic embryogenesis in

Coffea,” spp. 7th International Congress on Plant Tissue and Cell Culture. p. 261.

27. NAKAMURA, T. and E. MAEDA. 1992. “Studies on somatic embryogenesis of coffee by scanning

electron microscope.” Japan. Jour. Crop Sci. 61:476-486.

28. NAKAMURA, T., T. TANIGUCHI and E. MAEDA. 1992. “Leaf anatomy of (Coffea arabica L.) with

reference to domatia.” Japan. Jour. Crop Sci. 61:642-650.

29. NAKAMURA, T., T. TANIGUCHI and E. MAEDA. 1994. “Cyto-histological studies on somatic

embryos of coffee: Ultrastructural aspects.” Japan. Jour. Crop Sci. 63: 144-157.

30. TEIXEIRA, J.B., M. R. SONDAHL, T. NAKAMURA and E.G. KIRBY. 1995. “Establishment of oil

palm cell suspensions and plant regeneration.” Plant Cell,Tissue and Organ Culture 40: 105-111.


31. NAKAMURA, T., T. TANIGUCHI and E. MAEDA. 1995. “An ultrastuctural study on plantlet

development of Indica type rice tissue culture.” (submitted)

SPECIAL SKILLS

--Proficient in Portuguese, Japanese and English.

--Experienced plant photographer.

--Proficient with PC and Mac computers and Picasa image storage and manipulation software.

--Experienced teacher.

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