curriculum vitae of ayyoob arpanaei
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Curriculum Vitae
Personal Information
Mehdi Shamsara, Ph.D. in Molecular Genetics, Associated professor
Affiliation: National Institute of Genetic Engineering and Biotechnology (NIGEB),
Shahrak-e Pajoohesh, 17-km ofTehran-Karaj Highway, P.O. Box: 14965/161, Tehran,
Iran.
Phone (office): +98-21-44580414
Fax: +98-21-44580395
Email (academic): [email protected]
Email (private): [email protected]
Date of Birth: 01/07/1975
Place of Birth: Tehran, IRAN
Education
M.Sc. in Medical Biotechnology: Tarbiat Modares University, Tehran, Iran, 2001.
Ph.D. in Molecular Genetics, Tarbiat Modares University and NIGEB, Tehran, Iran,
2007.
Conference publications
1. Forouzandeh M., Shamsara M., Mousavi M.L. Detection of Clostridium botulinum
type A by PCR-ELISA technique. The 2nd National Congress of Biotechnology, 9-11
October 2001, Karaj, Iran.
2. Forouzandeh M., Mousavi M.L., Shamsara M. Detection of Clostridium botulinum
type A by solution hybridization method. 9th Asian-Pacific Congress of Clinical
Biochemistry, 9-14 March 2002, New Delhi, India.
3. Shamsara M., Ghorashi S.A., Ahmadian G., Momeni Moghaddam M., Angadji L.,
Salehi-Tabar R. Cloning and Expression of VP2 Gene of Infectious Bursal Disease
Virus in Yeast, 14th World Veterinary Poultry Congress, 22-26 August 2005, Istanbul,
Turkey.
4. Momeni Moghaddam M., Ghorashi S.A., Hosseini M., Salehi-Tabar R., Angadji L.,
Shamsara M., Detection of Avian Infectious Bronchitis by RT-PCR, 14th World
Veterinary Poultry Congress, 22-26 August 2005, Istanbul, Turkey.
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5. Angadji L., Ghorashi S.A., Asmar M., Momeni Moghaddam M., Shamsara M., Salehi-
Tabar R. Detection of Newcastle Disease Virus (NDV) by RT-PCR, 14th World
Veterinary Poultry Congress, 22-26 August 2005, Istanbul, Turkey.
6. Salehi-Tabar R, Ghorashi S.A., Shamsara M., Memari B. Reverse Transcription-
Polymersae Chain Reaction for the Detection of Avian Pneumovirus, 14th World
Veterinary Poultry Congress, 22-26 August 2005, Istanbul, Turkey.
7. Shamsara M., Ghorashi S.A., Ahmadian G., Salehi-Tabar R. Expression of VP2
Protein of Infectious Bursal Disease Virus (Gumboro) in Pichia pastoris. The 4th
National Biotechnology Congress, 15-17 August 2005, Kerman, Iran.
8. Memari B., Ghorashi S.A., Shamsara M., Aliabadizadeh K., Daliri M., Zomorodipour
A.R., Ebrahimipour G.R., Salehi-Tabar R. Cloning and sequencing of bovine growth
hormone gene of an Iranian cattle. The 4th National Biotechnology Congress, 15-17
August 2005, Kerman, Iran.
9. Shamsara M., Ghorashi S.A., Ahmadian G., Salehi-Tabar R. Development of a RT-
PCR-RFLP Assay for Differentiation Between Iranian Very Virulent Infectious Bursal
Disease Viruses and Reported Strains in the World. The 4th National Biotechnology
Congress, 15-17 August 2005, Kerman, Iran.
10. Shamsara M., Ghorashi S.A., Ahmadian G., Salehi-Tabar R. Cloning and
Sequencing of the VP2 Gene of a Very Virulent Infectious Bursal Disease Virus
(Gumboro). The 4th National Biotechnology Congress, 15-17 August 2005, Kerman,
Iran.
11. Saeedinia A.R., Shamsara M., Zeinoddini M., Sadeghi V. Detection of
Coxsackieviruse B3 in cell culture and animal tissue samples by RNA amplification
technique, The 3rd Iranian Congress of Virology, 21-23 January 2006, Tehran, Iran.
12. Shamsara M., Ghorashi S.A., Ahmadian G. Neutralization of Infectious Bursal
Disease Virus Using a VP2 Protein Expressed in Pichia pastoris. The 5th Convention of
Iranian Veterinary Clinicians, 12-14 February, 2008, Ahvaz, Iran.
13. Shamsara M., Ghorashi S.A., Mahravani H. Cloning and sequencing of capsid
genes of Foot-and-Mouth disease virus. The 15th Iranian Veterinary Congress, 26-28
April 2008, Tehran, Iran.
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14. Dadras S., Ghorashi S.A., Hosseini M., Dadras H., Shamsara M., Sequencing of
Segment A of a very virulent Infectious Bursal Disease Virus genome. First
International Veterinary Poultry Congress, 19-20 February 2008, Tehran, Iran.
15. Tafvizi F., Shamsara M., Yousefi T., Mahravani H., Harky nejad T., Ghorashi
S.A. Immunogenecity Study of DNA Vaccine for Foot-and-Mouth Disease in Mouse.
The 6th National Biotechnology Congress. 13-15 August, 2009, Tehran, Iran.
16. Yousefi T., Shamsara M., Tafvizi F., Mahravani H., Eskandarinasab M.,
Ghorashi S.A. Cloning and Phylogenetic Analyses of P12A and 3C genes of Foot-
and-Mouth Disease Virus. The 6th National Biotechnology Congress. 13-15 August,
2009, Tehran, Iran.
17. Babashpour S., Aminzadeh S., Farrokhi N., Khosroshahli M., Shamsara M.,
Keshavarz M., Ghoroghi A., Motallebi A. Functional Genomics of a Mesophilic
Chitinase Isolated from Shrimp Pond. The 3rd Iranian Proteomics conference, 26-27
May 2010, Tehran, Iran.
18. Sahar Sedighzadeh, Mehdi Shamsara, Azar Shahpiri. Expression of variable
region of VP2 gene of infectious bursal disease virus in Escherichia coli. 6th National
and 4th International Conference of Biology, 14-16 September 2010, Mashhad, Iran.
19. Babashpour S., Aminzadeh S., Farrokhi N., Khosroshahli M., Shamsara M.,
Keshavarz M., Ghoroghi A., Motallebi A. Heterologous expression and structural
investigation of chitinase from the chitinolytic bacterium Serratia marcescens B4A.
6th National and 4th International Conference of Biology, 14-16 September 2010,
Mashhad, Iran.
20. Arjmand S., Lotfi S.A., Bidram E., Shamsara M. Production and purification of
recombinant human alpha1-antitrypsin in methylotrophic yeast Pichia pastoris.
Accepted for ICBB 2011 (Japan).
21. Sedighzadeh S., Shamsara M., Shahpiri A., Haji-Khodadad. Extraction and
detection of hypervariable region of VP2 protein in Escherichia coli. The First
International Student Congress on Cell and Molecular Medicine, 17-19 february
2011, Shiraz, Iran.
22. Jafar Khezri, Farid Heidari, Mehdi Shamsara, Ehsan Hashemi, Aidin Rahim-
Tayefeh. Application of Morula aggregation technique for integration of embryonic
stem cells into inner cell mass of blastocyst. The 2nd Iranian Medical Genetics
Congress, 20-21 June 2011, Tehran, Iran.
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23. Jafar Khezri, Farid Heidari, Mehdi Shamsara, Ehsan Hashemi, Aidin Rahim-
Tayefeh. Production of chimeric mice containing knockout ADAM27 gene. The 2nd
Iranian Medical Genetics Congress, 20-21 June 2011, Tehran, Iran.
24. Vesagh Hamadani Z., Farokhi N., Heidari F., Shamsara M., Aminzadeh S.
Cloning and expression of recombinant IZUMO protein from Sperm. The 12th Iranian
Genetics Congress, 21-23 May 2012, Tehran, Iran.
25. Parvaneh Baktash, Mehdi Shamsara, Mohammad Massumi. Generation of
induced pluripotent stem (iPS) cells from mouse embryonic fibroblasts (MEFs) using
a doxycycline inducible system for producing reprogrammable iPS mouse. 12th
Iranian Genetics Congress, 21-23 May 2012, Tehran, Iran.
26. Fatemeh Farzaneh, Zohreh Mirzapour, Mehdi Shamsara. Perspective the use of
chromatin insulators in gene delivery and production of transgenic animals. 3rd
International Student Biotechnology Congress, 5-7 May 2013, Tehran, Iran.
27. Parvin Valimehr, Saeeid Aminzadeh, Mehdi Shamsara, Naser Farokhi. Isolation
and cloning of amylopullulanases enzyme 04159 from indigenous Cohnella A01. The
8th National Congress of Biotechnology, 6-8 July 2013, Tehran, Iran.
28. Jahangirian Ehsan, Fatemeh Farzaneh, Zohreh Mirzapour, Mehdi Shamsara.
Designing a genetic construct to generate a transgenic mouse carrying neomycin-
resistance gene. First International and 13th Iranian Genetics Congress. 24-26 May
2014, Tehran, Iran.
29. Valaee S., Yaghoobi M.M., Shamsara M. Phylogenetic study of vimentin gene in
order to suggesting an animal model. First International and 13th Iranian Genetics
Congress. 24-26 May 2014, Tehran, Iran.
30. Valimehr S., Zomorodipour A.R., Shamsara M., Hashemi E. Construction a
targeting vector to generate a knockout mouse in FVIII gene. First International and
13th Iranian Genetics Congress. 24-26 May 2014, Tehran, Iran.
31. Pilehchian Langroudi R., Aghaei Pour
K., Shamsara
M. A Bioinformatics
approach to produce a fusion chimeric protein of ε-β toxin of Clostridium perfringens
and its expression in Escherichia coli. The 4th Iranain Conference on Bioinformatics,
6-7 November 2012, Tehran, Iran.
32. Poyanfar F., Dashtizad M., Shamsara M., Forotan T. Effect of artificially
collapse of blastocoelic cavity before vitrification on pluripotency of inner cell mass
by studying the expression of klf4 gene. First International and 13th Iranian Genetics
Congress. 24-26 May 2014, Tehran, Iran.
33. Hosseini M., Karkhane A., Shamsara M., Yakhchali B., Farrokh P., Goodarzi N.,
Ghafoori M., Karimi E. Cloning a mutant Geobacillus thermocatenulatus lipase (Btl2)
in Pichia pastoris. First International & 12th Iranian Congress of Microbiology. 23 to
26 May 2011, Kermanshah, Iran.
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34. Ahmadian G., Shamsara M., Karbalayi Ali M., Haji-Khodaad S. Using
recombinant E.coli as a novel vaccine against foot and mouth disease virus. 22nd
European Congress of Clinical Microbiology and Infectious Diseases. 31 March 3 April
2012, London, UK.
35. Gharibi S., Khateri S., Soroush M.R., Mirzadeh F., Neali P., Shamsara M.,
Tavallaie M., S.J., Mowla. MicroRNA Expression Profiling in sulfur mustard exposed
patients: biological significance and diagnostic utility. First International and 13th
Iranian Genetics Congress. 24-26 May 2014, Tehran, Iran.
36. Hajikhodadad S., Shamsara M., Ahmadian G. Surface Display of peptides
derived from VP1 protein of Foot and Mouth Disease in E.coli. First International and
13th Iranian Genetics Congress. 24-26 May 2014, Tehran, Iran.
37. Kazemi P., Dashtizad M., Ahangari G., Shamsara M., Mahdavinezhad F.,
Hajarian H. Effect of in vitro fertilization on primitive endoderm specific genes
expression in mouse blastocysts. 20th Congress on Infertility and Reproduction, 11-
13 June 2014, Isfahan, Iran.
38. Bolouri R., Dashtizad M., Shamsara M., Daliri M., Hashemi M., Fathealizadeh P.,
Zandi G., Hojjatzadeh Dezfuli Z., Hajarian H. Effect of in vitro fertilization on
expression of apoptotic specific gene, Bax and Bcl2 in IVF-derived versus in vivo.
20th Congress on Infertility and Reproduction, 11-13 June 2014, Isfahan, Iran.
39. Fathealizadeh P., Dashtizad M., Daliri M., Shamsara M., Assadi Tehrani G.,
Zandi G., Rahim Tayefe A., Hajarian H. Quality assessment of blastocysts artificiality
collapsed by microneedle. 20th Congress on Infertility and Reproduction, 11-13 June
2014, Isfahan, Iran.
40. Hojjatzadeh Z., Dashtizad M., Shamsara M., Hashemi M., Bolouri R., Zandi G.,
Fathealizadeh P., Hajarian H. Effect of in vitro fertilization on expression of apoptotic
specific gene in mouse embryos at blastocysts stage. 20th Congress on Infertility and
Reproduction, 11-13 June 2014, Isfahan, Iran.
41. Zandi G., Dashtizad M., Daliri M., Shamsara M., Assadi Tehrani G.,
Fathealizadeh P., Hashemi E., Hajarian H. Expression of Oct4 gene in IVF-derived
mouse blastocyst. 20th Congress on Infertility and Reproduction, 11-13 June 2014,
Isfahan, Iran.
42. Mahdavinezhad F., Dashtizad M., Kabirsalmani M., Shamsara M., Kazemi P.,
Hajarian H. Effect of artificially collapse of blastocoelic cavity on viability of mouse
blastocyst through Cdx2 gene expression. 20th Congress on Infertility and
Reproduction, 11-13 June 2014, Isfahan, Iran.
43. Mirzapoor Z., Farzaneh F., Shamsara M. Designing a genetic construct
expressing EGFP protein using Oct4 promoter to generate a transgenic mouse. 1st
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Tabriz International Life Science Conference and 12th Biophysical Chemistry
Conference. 22-24 May 2013, Tabriz, Iran.
44. Salami S., Shamsara M., Massumi M., Baktash P. The study of differentiation of
Nurr1, Piitx3-expressing mouse induced pluripotent stem cells (iPSCs) into
dopaminergic-like cells. 10th Royan International Congress on Stem Cell Biology and
Technology. 3-5 September, 2014.
45. Rajaei B., Massumi M., Sanati M.H., Taha M.F., Shamsara M., Motlagh F.M.
Efficient programming of human induced pluripotent stem cells derived from
fibroblast of diabetic patient to definitive endoderm-like cells. 10th Royan
International Congress on Stem Cell Biology and Technology. 3-5 September, 2014.
46. Fathealizadeh P., Dashtizad M., Daliri M., Shamsara M., Zandi G., Hashemi E.,
Bolouri R., Hajarian H., The effect of vitrification on quality of IVF-derived
blastocysts. The comprehensive National Congress of Novel Findings of Biology, 8-9
May 2014.
47. Hashemi E., Akhavan O., Shamsara M., Daliri M., Dashtizad M., Rahim-Tayefe
A. Graphene sheets induce geno- and cyto-toxicity in mouse spermatozoa. The
comprehensive National Congress of Novel Findings of Biology, 8-9 May 2014.
48. Zandi G., Dashtizad M., Daliri M., Shamsara M., Fathealizadeh P., Rahim Tayefe
A., Hojjatzadeh Dezfuli Z., Hajarian H. Artificial collapse of in vivo mouse blastocyst
using microneedle and its impact on survival and hatching rates. The Comprehensive
National Congress of Novel Findings of Biology, 8-9 May 2014.
49. Bolouri R., Dashtizad M., Shamsara M., Daliri M., Hashemi M., Hojjatzadeh
Dezfuli Z., Hajarian H. Effect in vitro fertilization on expression of apoptotic specific
genes in mouse embryos at blastocysts stage. The Comprehensive National Congress
of Novel Findings of Biology, 8-9 May 2014.
50. Hojjatzadeh Dezfuli Z., Dashtizad M., Shamsara M., Hashemi M., Zandi G.,
Fathealizadeh P., Bolouri R., Hajarian H. Effect of vitrification on expression of
apoptotic specific gene p53 in IVF-derived versus in vivo embryos. The
Comprehensive National Congress of Novel Findings of Biology, 8-9 May 2014.
51. Hashemi E., Akhavan O., Shamsara M., Daliri M., Dashtizad M., Rahim-Tayefe
A. Cytotoxity of grapheme nanoplatelets on spermatogonial stem cells (a Bio-nano
interaction). The Comprehensive National Congress of Novel Findings of Biology, 8-9
May 2014.
52. Rajaei B., Massumi M., Taha M.F., Shamsara M., Generation of human induced
pluripotent stem cells by reprogramming of adult human fibroblasts with a four
transcription factor, doxycycline inducible lentiviral transfection System. 12th
International Congress of Immunology & Allergy of Iran. 29th April-2nd May 2014,
Tehran, Iran.
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53. Pilechian R., Aghaei-pour K., Shamsara M. Synthetic construct of Clostridium
perfringens epsilon-beta fusion protein gene. 4th International Conference on
Biotechnology and environmental Management. 23-25 September, 2011, Singapore.
54. Arjmand S., Bidram E., Lotfi A., Shamsara M., Ghaedi M., Adibi B., Mowla J.
Study the effect of different media on cell growth and recombinant protein
expression in Pichia pastoris The 10th Iranian Congress Of Biochemistry &The 3nd
International Congress Of Biochemistry And Molecular Biology. 16-19 November,
Tehran, Iran.
Journal Publications
1. Forouzandeh M., Shamsara M., Mousavi M.L. Detection of Clostridium botulinum
type A by PCR-ELISA. Modares Journal of Medical Sciences, 4 (2001) 157-165. (in
Persian).
2. Forouzandeh M., Shamsara M., Mousavi M.L. Detection of Clostridium botulinum
type A by solution hybridization method. Trends in the clinical biochemistry and
laboratory, (2003) 381-383.
3. Ahmadian G., Shamsara M., Easton A.J. Pneumoviruses: Molecular Genetics and
Reverse Genetics. Iranian Journal of Biotechnology, 3 (2005) 78-93.
4. Shamsara M., Ghorashi S.A., Ahmadian G. Cloning and Nucleotide Analysis of the
VP2 Gene of Very Virulent Infectious Bursal Disease Virus Isolated from Iran. Acta
Virologica, 50 (2006) 229-234.
5. Hashemi M., Ghorashi S.A., Parivar M., Dizaji A., Shamsara M., Doostar Y.,
Bakhshayesh M. Induction of Apoptosis in Malignant Human B Lymphocyte by
Recombinant VP2 Protein of Infectious Bursal Disease Virus. Medical Sciences Journal
of Islamic Azad University, 16 (2007) 191-199. (in Persian)
6. Saeedinia A., Shamsara M., Zeinoddini M., Sadeghi V., Maghsoudi N. Evaluation of
nucleic acid sequence based amplification (NASBA) and Reverse Transcription
Polymerase Chain Reaction for detection of coxsackievirus B3 in cell culture and
animal tissue samples. Iranian Journal of Biotechnology, 6 (2008) 222-228.
7. Saeedinia A., Shamsara M., Bahrami A., Zeinoddini M., Naseeri-Khalili M.A.,
Mohammadi R., Malek Sabet M., Sami H. Heterologous Expression of Human
Granulocyte-Colony Stimulating Factor in Pichia pastoris. Biotechnology, 7 (2008)
569-573.
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8. Ghaedi M., Lotfi A.S., Soleimani M., Shamsara M., Arjmand A., Adibi B. Expression
of recombinant alpha-1 antitrypsin in CHO and COS-7 Cell lines using lentivirally
vector. Iranian Journal of Biotechnology, 7 (2009) 148-156.
9. Arjmand S., Bidram E., Lotfi A., Shamsara M., Ghaedi M., Adibi B., Mowla J.,
Study the effect of different media on cell growth and recombinant protein expression
in Pichia pastoris. Journal of the Iranian Chemical Society, 6 (Suppl) (2009) S230-
S237.
10. Lashkarian H.E., Raheb J., Shahzamani K., Shahbani H., Shamsara M.,
Hajipour M. Isolation and identification of a native Rhodococcus strain producing
cholesterol oxidase from soil. International Journal of Biotechnology and
Biochemistry, 6 (2010) 203-215.
11. Arjmand S., Lotfi S.A., Shamsara M., Bidram E. Recombinant human alpha1-
antitrypsin expression in Pichia pastoris and nanoencapsulation. G.I.T. Laboratory
Journal, 2010.
12. Lashkarian H., Raheb J., Shahzamani K., Shahbani H., Shamsara M.
Extracellular cholesterol oxidase from Rhodococcus sp.: isolation and molecular
characterization. Iranian Biomedical Journal 14 (2010) 49-57.
13. Tabatabaei M., Zakaria M.R., Abdul Rahim R., Abdullah N., Wright A.G.,
Yoshihito Shirai, Shamsara M., Sakai K., Hassan M.A. Comparative study of
methods for extraction and purification of environmental DNA from high-strength
wastewater sludge. African Journal of Biotechnology. 9 (2010) 4926-4937.
14. Hashemi M., Entezari M., Mohammadnejad L., Jamshidian F., Shamsara M.,
Soleimani M. Cloning of Oct3/4 gene in embryonic stem cells. Journal of Paramedical
Sciences, 2 (2011) 41-46.
15. Pilehchian Langroudi R., Aghaei Pour
K., Shamsara
M., Jabbari
A.R., Habibi
G.R.,
Goudarzi
H., Ghorashi
S.A. Fusion of Clostridium perfringens type D and B epsilon
and beta toxin genes and it’s cloning in E. coli. Archives of Razi Institute, 66 (2011)
1-10.
16. Nayebi N., Ghorashi S.A., Harzandi N., Shamsara M., Tabarai B., Bakhtiyari A.
Diagnostic value of PCR method for detection of Salmonella enteritidis contamination
in poultry products in Karaj. Medical Sciences Journal of Islamic Azad University, 21
(2011) 32-37. (in Persian)
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17. Arjmand S., Bidram E., Sahebghadam Lotfi A., Shamsara M., Mowla J.
Expression and Purification of Functionally Active Recombinant Human Alpha 1-
Antitrypsin in Methylotrophic Yeast Pichia pastoris. Avicenna Journal of Medical
Biotechnology, 3 (2011) 127-134.
18. Pilehchian Langroudi R., Aghaiypour K., Shamsara M., Ghorashi S.A. In silico
fusion of epsilon and beta toxin genes of Clostridium perfringens types D and B.
Iranain Journal of Biotechnology, 10 (2012) 54-60.
19. Ebrahimi MM., Shahsavandi S., Moazenijula G., Shamsara M. Phylogeny and
evolution of Newcastle disease virus genotypes isolated in Asia during 2008-2011.
Virus Genes, 45 (2012) 63-68.
20. Sedighzadeh SS., Shamsara M., Haji Khodadad S. Cloning and codon optimized
expression of structural protein hypervariable region of VP2 from infectious bursal
disease virus. International Journal of Molecular and Clinical Microbiology, 2 (2012)
119-123.
21. Sedighzadeh SS., Shamsara M., Shahpiri A. Fusion Protein Strategy to Increase
Expression and Solubility of Hypervariable Region of VP2 Protein of Infectious Bursal
Disease Virus in Escherichia coli. Protein Journal, 31 (2012) 580-584.
22. Hosseini M., Karkhane AA., Yakhchali B., Shamsara M., Aminzadeh S.,
Morshedi D., Haghbeen K., Torktaz I., Karimi E., Safari Z. In Silico and Experimental
Characterization of Chimeric Bacillus thermocatenulatus Lipase with the Complete
Conserved Pentapeptide of Candida rugosa Lipase. Applied Biochemstry and
Biotechnology, 169 (2012) 773-785.
23. Arjmand S., Sahebghadam Lotfi A., Shamsara M., Mowla SJ. Elevating the
expression level of biologically active recombinant human alpha 1-antitrypsin in
Pichia pastoris. Electronic Journal of Biotechnology, 16 (2013).
24. Esmaelizad M., Ahmadian G., Aghaiypour K., Shamsara M., Paykari H.,
Tebianian M. Induction of prominent Th1 response in C57Bl/6 mice immunized with
an E. coli-expressed multi T-cell epitope EgA31 antigen against Echinococcus
granulosus. Folia Parasitologica 60 (2013) 28-34.
25. Pilehchian Langroudi R., Shamsara M., Aghaiypour K. Expression of Clostridium
perfringens epsilon-beta fusion toxin gene in E. coli and its immunologic studies in
mouse. Vaccine 31 (2013) 3295-3299.
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26. Esmaelizad M., Ahmadian G., Aghaiypour K., Shamsara M., Paykari H.,
Tebianian M. Induction of protective T-helper 1 immune responses against
Echinococcus granulosus in mice by a multi-T-cell epitope antigen based on five
proteins. Memórias do Instituto Oswaldo Cruz 108 (2013) 408-413.
27. Hashemi E., Akhavan O., Shamsara M., Rahighi R., Esfandiar A., Rahim
Tayefeh A. Cyto and genotoxicities of graphene oxide and reduced graphene oxide
sheets on spermatozoa. RSC Advance 4 (2014) 27213-27223.
28. Hashemi E., Akhavan O., Shamsara M., Valimehr S., Rahighi R. DNA and RNA
extractions from eukaryotic and prokaryotic cells by graphene nanoplatelets. RSC
Advance 4 (2014) 60720-60728.
29. Goodarzi N., Karkhane A.A., Mirlohi A., Tabandeh F., Torktas I., Aminzadeh S.,
Yakhchali B., Shamsara M., Ghafouri M.A. Protein engineering of Bacillus
thermocatenulatus lipase via deletion of the α5 helix. Applied Biochemistry
Biotechnology. 1 (2014) 339-351.
30. Fatemi N., Sanati M.H., Shamsara M., Moayer F., Zavarehei M.J., Pouya A.,
Sayyahpour F., Ayat H., Gourabi H. TBHP-induced oxidative stress alters microRNAs
expression in mouse testis. Journal of Assisted Reproduction and Genetics 31 (2014)
1287-1293.
31. Yosefi T., Tafvizi F., Shamsara M., Harkynejad T., Ghorashi S.A., Mahravani H.
Improve immunogenicity of DNA vaccine against foot-and-mouth disease virus: Role
of intron and probably viral 3C protease as biological adjuvants. Molecular Genetics,
Microbiology and Virology 29 (2014) 154-157.
32. Karimi E., Karkhane A.A., Yakhchali B., Shamsara M., Aminzadeh S., Torktaz
I., Hosseini M., Safari Z. Study of the effect of F17A mutation on characteristics of
Bacillus thermocatenulatus lipase expressed in Pichia pastoris using in silico and
experimental methods. Biotechnology and Applied Biochemistry 61 (2014) 264-273.
33. Gharbi S., Mirzadeh F., Khatrei S., Soroush M.R., Tavallaie M., Nourani M.R.,
Shamsara M., Mowla S.J. Optimizing microRNA quantification in serum samples.
Journal of Cell and Molecular Research 6 (2014) 52-56.
34. Zeinoddini M., Saeedinia A.R., Sadeghi V. Shamsara M., Hajia M. and Rahbar
M. Simple and accurate detection of Vibrio cholera using triplex dot blotting assay.
Biomacromolecular Journal 1 (2015) 52-57.
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35. Gharbi S., Mowla S.J., Khatrei S., Soroush M.R., Ghorban Mehr N., Tavallaie
M., Nourani M.R., Shamsara M. Identification of reliable reference genes for
microRNAs quantification in serum samples of sulfur mustard-exposed veterans. Cell
Journal 17 (2015) 494-501
36. Ghafouri M.S., Karkhane A.A., Azimi M.R., Yakhchali B., Shamsara M., Cloning,
expression and purification of a mutant lipase from Geobacillus thermocatenulatus in
Pichia pastoris. Genetics Novin 4 (2015) 403-410.
37. Askari N., Yaghoobi M.M., Shamsara M., Esmaeili-Mahani S. Human dental pulp
stem cells differentiate into oligodendrocyte progenitors using the expression of
Olig2 transcription factor. Cells Tissues Organs 200 (2014) 93-103.
38. Askari N., Karam-Zadeh N., Yaghoobi M.M., Shamsara M. Development of
human dental pulp stem cells expressing reverse tetracycline-controlled trans
activator by different transfection methods. Online Journal of Veterinary Research 19
(2015) 137-147.
39. Askari N, Yaghoobi MM, Shamsara M, Esmaeili-Mahani S. Tetracycline-
regulated expression of OLIG2 gene in human dental pulp stem cells lead to mouse
sciatic nerve regeneration upon transplantation. Neuroscience. 305 (2015) 197-208.
40. Askari N, Karam-Zadeh N, Yaghoobi MM, Shamsara M. Development of human
dental pulp stem cells expressing reverse tetracycline-controlled trans activator by
different transfection methods. Onl J Vet Res. 2015, 19: 137-147.
41. Soltani L, Rahmani HR, Daliri Joupari M, Ghaneialvar H, Mahdavi AH, Shamsara
M. Ovine fetal mesenchymal stem cell differentiation to cardiomyocytes, effects of
co-culture, role of small molecules; reversine and 5-azacytidine. Cell Biochemstry
and Function (2016) doi: 10.1002/cbf.3187.
42. Rezaei A, Akhavan O, Hashemi E, Shamsara M. Ugi Four-Component Assembly
Process: An Efficient Approach for One-Pot Multifunctionalization of Nanographene
Oxide in Water and Its Application in Lipase Immobilization. Chemstry of Materials
(2016) doi: 10.1021/acs.chemmater.6b00099.
43. Salemi S, Baktash P, Rajaei B, Noori M, Amini H, Shamsara M, Massumi M.
Efficient generation of dopaminergic-like neurons by overexpression of Nurr1 and
Pitx3 in mouse induced Pluripotent Stem Cells. Neurosci Lett. 2016 Jul 28;626:126-
34. doi: 10.1016/j.neulet.2016.05.032.
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44. Rajaei B, Shamsara M, Taha MF, Amirabad LM, Massumi M, Sanati MH.
Pancreatic Endoderm-Derived From Diabetic Patient-Specific Induced Pluripotent
Stem Cell Generates Glucose-Responsive Insulin-Secreting Cells. J Cell Physiol. 2016
Jun 16. doi: 10.1002/jcp.25459.
45. Kazemi P, Dashtizad M, Shamsara M, Mahdavinezhad F, Hashemi E, Fayazi S,
Hajarian H. Effect of blastocoel fluid reduction before vitrification on gene expression
in mouse blastocysts. Mol Reprod Dev. 2016 Aug;83(8):735-42. doi:
10.1002/mrd.22681.
46. Hashemi E, Akhavan O, Shamsara M, Daliri M, Dashtizad M, Farmany A.
Synthesis and cyto-genotoxicity evaluation of graphene on mice spermatogonial
stem cells. Colloids Surf B Biointerfaces. 2016 Oct 1;146:770-6. doi:
10.1016/j.colsurfb.2016.07.019.
47. Rezaei A, Akhavan O, Hashemi E, Shamsara M. Toward Chemical Perfection of
Graphene-Based Gene Carrier via Ugi Multicomponent Assembly Process.
Biomacromolecules. 2016 Sep 12;17(9):2963-71. doi:
10.1021/acs.biomac.6b00767.
48. Akhavan O, Hashemi E, Zare H, Shamsara M, Taghavinia N, Heidari F.
Influence of heavy nanocrystals on spermatozoa and fertility of mammals. Mater Sci
Eng C Mater Biol Appl. 2016 Dec 1;69:52-9. doi: 10.1016/j.msec.2016.06.055.
49. Ahmadian H, Hashemi E, Akhavan O, Shamsara M, Hashemi M, Farmany A,
Joupari MD. Apoptotic and anti-apoptotic genes transcripts patterns of graphene in
mice. Mater Sci Eng C Mater Biol Appl. 2017 Feb 1;71:460-464. doi:
10.1016/j.msec.2016.09.073.
50. Mohammadi Amirabad L, Massumi M, Shamsara M, Shabani I, Amari A,
Mossahebi Mohammadi M, Hosseinzadeh S, Vakilian S, Steinbach SK, Khorramizadeh
MR, Soleimani M, Barzin J. Enhanced Cardiac Differentiation of Human
Cardiovascular Disease Patient-Specific Induced Pluripotent Stem Cells by Applying
Unidirectional Electrical Pulses Using Aligned Electroactive Nanofibrous Scaffolds.
ACS Appl Mater Interfaces. 2017 Feb 15. doi: 10.1021/acsami.6b15271.
51. Farmany A, Shamsara M, Mahdavi H. Enhanced electrochemical biosensing of
Buprenorphine opioid drug by highly stabilized magnetic nanocrystals. Sensors and
Actuators B: Chemical 2017, 239, 279-285.
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52. Valaee S, Yaghoobi MM, Shamsara M. Metformin inhibits gastric cancer cells
metastatic traits through suppression of epithelial-mesenchymal transition in a
glucose-independent manner. PLoS One. 2017, 12, e0174486. doi:
10.1371/journal.pone.0174486.
53. Amini-Farsani Z, Sangtarash MH, Shamsara M, Teimori H. MiR-221/222
promote chemoresistance to cisplatin in ovarian cancer cells by targeting
PTEN/PI3K/AKT signaling pathway. Cytotechnology. 2018, 70, 203-213. doi:
10.1007/s10616-017-0134-z.
54. Amirrasouli MM, Shamsara M. Comparing the in vivo and in vitro effects of
hypoxia (3% O2) on directly derived cells from murine cardiac explants versus
murine cardiosphere derived cells. J Stem Cells Regen Med. 2017, 13, 35-44.
55. Amini-Farsani Z, Sangtarash MH, Teimori H, Shamsara M. Effect of valproic acid
on Expression of Bim gene and viability of ovarian cancer cell line A2780.
J Gorgan Univ Med Sci. 2017, 19: 60-66.
56. Sarmadi F, Esfandiari S, Dashtizad M, Shamsara M. The effect of using natural
honey as new cryoprotectant on vitrified-warmed blastocyst s survival, hatching and
implantation rate. Journal of Animal Biology 2017, 9: 25-34.
57. Rahimnahal S, Meimandipour A, Fayazi J, Karkhane AA, Shamsara M,
Taghi Beigi Nassiri M. Keratinase producing bacteria isolated from litter for digestion
of keratin in feathers. Onl J Vet Res. 2017, 21: 335-344.
58. Rajaei B, Shamsara M, Sanati MH. In Vitro Generation of Glucose-Responsive
Insulin-Secreting Cells from Pancreatic and Duodenal Homeobox 1-Overexpressing
Human-Induced Pluripotent Stem Cell Derived from Diabetic Patient. ASAIO J. 2018,
64, 819-826.
59. Sajadpoor Z, Amini-Farsani Z, Teimori H, Shamsara M, Sangtarash MH,
Ghasemi-Dehkordi P, Yadollahi F. Valproic Acid Promotes Apoptosis and Cisplatin
Sensitivity Through Downregulation of H19 Noncoding RNA in Ovarian A2780 Cells.
Appl Biochem Biotechnol. 2018, doi: 10.1007/s12010-017-2684-0.
60. Gharbi S, Khateri S, Soroush MR, Shamsara M, Naeli P, Najafi A, Korsching E,
Mowla SJ. MicroRNA expression in serum samples of sulfur mustard veterans as a
diagnostic gateway to improve care. PLoS One. 2018, 13, e0194530. doi:
10.1371/journal.pone.0194530.
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61. Bagheri D, Kazemi P, Sarmadi F, Shamsara M, Hashemi E, Daliri Joupari M,
Dashtizad M. Low oxygen tension promotes invasive ability and embryo implantation
rate. Reprod Biol. 2018,18:295-300. doi: 10.1016/j.repbio.2018.05.003.
62. Khezri J, Heidari F , Shamsara M. A study on merits and demerits of two main
gene silencing techniques in mice. Iranian J Biotech. 2018,16, e1632. doi:
10.21859/ijb.1632.
63. Kohram F, Fallah P, Shamsara M, Bolandi Z, Rassoulzadegan M, Soleimani M,
Ghanbarian H. Cell type-dependent functions of microRNA-92a. J Cell Biochem.
2018, 119, 5798-5804.
64. Ansari F, Nikougoftar Zarif M, Hamidieh AA, Shamsara M. Generation of K562
cell line expressing Cas9 endonuclease (CRISPR-associated9). Sci J Iran Blood
Transfus Organ 2018; 16: 32-43.
65. Soltani, L., Rahmani, H.R., Daliri Joupari, M. Ghaneialvar H, Mahdavi َ AH,
Shamsara M. Effects of Different Concentrations of Reversine on Plasticity of
Mesenchymal Stem Cells. Ind J Clin Biochem. 2018. Doi: 10.1007/s12291-018-
0800-8.
66. Saffari M, Heidari F, Shamsara M, Hashemi E. The Effect of Different Factors on
X-and Y Bearing Sperm in Bull Semen. IRAN J VET RES 2018, 12: 219-227.
67. Valiulahi P, Aminzadeh S, Shamsara M, Farrokhi N, Alikhajeh J. Heterologous
expression and characterization of a recombinant thermostable amylopullulanases
Coh4159 from Cohnella sp. A01. ARMMT, 2018, 1: 31-38.
68. Rahimnahal S, Fayazi J, Meimandipour A, Beigi Nassiri MT, Shamsara M,
Karkhane AA. Keratinase gene Cloning in Escherichia coli and investigating some of
its bioinformatics features. MGj 2018, 12: 577-585.
69. Farzaneh F, Mirzapoor Z, Jahangirian E, Heidari F, Hashemi E, Rahim-Tayefeh A,
Fatemi N, Jamshidizad A, Dashtizad M, Shamsara M. The chicken hypersensitive
site-4 insulator cannot fully shield the murine phosphoglycerate kinase-1 promoter
from integration site effects in transgenic mice. 3 Biotech. 2019, 9, 255. doi:
10.1007/s13205-019-1786-5.
70. Ghassemi B, Ajami M, Shamsara M, Soleimani M, Kiani J, Rassoulzadegan M.
Pipeline for the generation of gene knockout mice using dual sgRNA CRISPR/Cas9-
mediated gene editing. Anal. Biochem. 2019, 568, 31-40.
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71. Eskandarian Boroujeni M, Alizadeh Miran R, Afkhami F, Tavakol Z, Shamsara M.
Genetically modified mice-Methods, applications and outlook. SJKU 2019, 24, 24-44
(In Persian).
72. Hashemi-Sheikhshabani S, Amini-Farsani Z, Shamsara M, Sajadpoor Z,
Sangtarash MH, Teimori H. Effect of valproic acid on cisplatin-resistant ovarian
cancer cell lines. J Shahrekord Univ Med Sci. 2019, 21:39-44.
73. Eskandarian Boroujeni M, Alizadeh R, Afkhami F, Tavakol Z, Shamsara M.
Genetically modified mice-Methods, applications and outlook. SJKU 2019, 24: 24-44.
74. Hosseinzadeh Shirzeyli M, Amidi F, Shamsara M, Nazarian H, Eini F, Majidi
Zolbin M, Ghaffari novin M, Daliri Joupari M. Exposing Mouse Oocytes to MitoQ
During In Vitro Maturation Improves Maturation and Developmental Competence.
Iranian Journal of Biotechnology.
75. Mosallatpour S, Aminzadeh S, Shamsara M, Hajihosseini R. Novel halo- and
thermo-tolerant Cohnella sp. A01 L-glutaminase: heterologous expression and
biochemical characterization. Sci Rep. 2019, 9:19062. doi: 10.1038/s41598-019-
55587-9.
76. Hashemi E, Mahdavi H, Khezri J, Razi F, Shamsara M, Farmany A. Enhanced
Gene Delivery in Bacterial and Mammalian Cells Using PEGylated Calcium Doped
Magnetic Nanograin. Int J Nanomedicine. 2019,14: 9879-9891. doi:
10.2147/IJN.S228396.
77. Valaee Sh, Yaghoobi MM, Jamshidizad A, Shamsara M. Glucose dependent
effect of metformin on expression of two microRNAs-related to epithelial to
mesenchymal transition in a gastric cancer cell line. Journal of Cellular and Molecular
Research (Iranian Journal of Biology) 2019, 32: 225–235.
78. Ghanei Z, Jamshidizad A, Joupari MD, Shamsara M. Isolation and
characterization of breast cancer stem cell-like phenotype by Oct4 promoter-
mediated activity. J Cell Physiol. 2020. doi: 10.1002/jcp.29437.
Books
Jafar Kiani, Mehdi Shamsara. Principles of Recombinant Protein Expression in
Escherichia coli. 1st edition, Andisheye Zohor, 1384. (in Persian)
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Syed Yonus Hosseini, Pooneh Mokaram, Hemidreza Miri, Mehdi Shamsara, et al.,
Gene Therapy: Concepts, Methods and Applications. 1st edition, Noor-Danesh, 1397. (in
Persian)
Patents
1. Meisam Tabatabaei, Mohd Rafein Zakaria, Raha Abdul Rahim, André-Denis G.
Wright, Yoshihito Shirai, Norhani Abdullah, Mehdi Shamsara, Kenji Sakai and Mohd
Ali Hassan. Method or Direct Isolation of High Yield, PCR-Compatible DNA from
Environmental Samples. 2008, Malaysian Patent PI20082842; PCT/MY2009/000143.
2. Sareh Arjmand, Abbas Sahebghadam Lotfi, Mehdi Shamsara. Engineering of 1-
antitrypsin gene in order to increase protein expression in Pichia pastoris. 2010,
Iranian patent, No: 63976.
3. Mohd Ali Hassan, Meisam Tabatabaei, Mohd Rafein Zakaria, Raha Abdul Rahim,
Andre-Denis G. Wright, Yoshihito Shirai, Norhani Abdullah, Mehdi Shamsara,
Kenji Sakai. Method for isolating DNA. 2011, WIPO Patent Application
WO/2011/031127.
4. Ehsan Hashemi, Omid Akhavan, Mehdi Shamsara. The use of graphene
nanoparticles for extraction of genomic DNA form mammalian cells. 2014 Iranian
patent, No: 84211.
5. Alireza Zomorodipour, Mehdi Shamsara, Abbas Jamshidizad, Maryam Kabirsalmani,
Faremeh Masoumi, Aidin Rahim-Tayefeh, Ehsan Hashemi. Production of FVIII
knockout mouse lines by means of CRISPR/Cas9 technology. 2019 Iranian patent,
No: 97989.
Research Interests
Gene therapy, stem cell and transgenesis.
Gene editing in cell lines and animal models.
Recombinant protein expression in prokaryotic and eukaryotic systems.
Honors and Awards
First rank among 1998 B.Sc. graduates of Microbiology.
First rank among 2001 M.Sc. graduates of Medical Biotechnology.
Generation of kinds of transgenic mice in the country.
17
Active Research Areas
1. Producing human induced pluripotent stem (iPS) cells from diabetic patients.
2. Designing and development genetically-modified mouse models, such as:
- Generation a transgenic mouse line expressing neomycin-resistance (neoR) gene
to produce neoR mouse embryonic fibroblasts (MEFs).
- Development a mouse model for hemophilia A disease by knockouting blood
coagulation factor VIII gene.
- Generation of a male infertility mouse model by inactivation of Spata19 gene
using CRISPR technology.