cultivation of viruses
DESCRIPTION
E. &. O. O. &. Cultivation of viruses. Cultivation. To propagate the viral particle in its specific host i.e. Increase viral concentration through support viral replication. Cultivation. Bacteria. Animal. Insect. Plant. Methods of animal virus cultivation. Organized tissue. - PowerPoint PPT PresentationTRANSCRIPT
CultivationCultivation
To propagate the viral particle in its specific host i.e. Increase viral concentration through support viral replication.
Cultivation
Bacteria
Plant
AnimalInsect
Methods of animal virus Methods of animal virus cultivationcultivation
1.Organized tissue.2.Cell culture.3.Laboratory animals.
Chick embryo technique Chick embryo technique
Methods of animal virus Methods of animal virus cultivationcultivation
1.Organized tissue.2.Cell culture.3.Laboratory animals.
Cell cultureCell culture
1. Removal.2. Homogenization.3. Washing.4. Trypsinization.5. Washing.6. Growing.7. Inoculation.8. Incubation.
Methods of animal virus Methods of animal virus cultivationcultivation
1.Organized tissue.2.Cell culture.3.Laboratory animals.
IntracerebrallyIntracerebrally
IntraperitoneallyIntraperitoneally
IntramuscularlyIntramuscularly
IntravenouslyIntravenously
IntranasallyIntranasally
Types of cell culture
Primary
Diploid
Suspension Permanent
Primary cell culturePrimary cell culture
A culture derived directly from a tissueA culture derived directly from a tissue
Diploid cell cultureDiploid cell culture
Subsequent cultures derived from primary cell culture.Subsequent cultures derived from primary cell culture.
Suspension cell cultureSuspension cell culture
A culture in it the dispersed cells are not allowed to settle down, but A culture in it the dispersed cells are not allowed to settle down, but they are kept floating by continuous shaking of the flasks.they are kept floating by continuous shaking of the flasks.
Permanent cell linePermanent cell line
A culture derived from single separated cell i.e. diploid culture it can be A culture derived from single separated cell i.e. diploid culture it can be propagated indefinitely. propagated indefinitely.
Cultivation of plant Cultivation of plant virusesviruses
• Protoplast and callus method.• Mechanical method.• Biological method.
Cultivation of plant Cultivation of plant virusesviruses
• Protoplast and callus method.• Mechanical method.• Biological method.
InoculationInoculation
IncubationIncubation
InoculationInoculation
IncubationIncubation
Cultivation of plant Cultivation of plant virusesviruses
• Protoplast and callus method.• Mechanical method.• Biological method.
Cultivation of plant Cultivation of plant virusesviruses
• Protoplast and callus method.• Mechanical method.• Biological method.
IncubationIncubation
Cultivation of bacterial Cultivation of bacterial virusesviruses
1.1. On broth mediaOn broth media 2. On solid medium2. On solid medium
Bacteria + solid agarBacteria + solid agar
Virus + semisolid agarVirus + semisolid agar
Cultivation of Insect virusesCultivation of Insect viruses
Criteria of purityCriteria of purity
Chemical purityChemical purity
Physical purityPhysical purity
Biological purityBiological purity