CryptomonadalesCryptomonadales®®: a unique species of Chlorella s: a unique species of Chlorella sorokiniana developed by MR. Shun-Te Wangorokiniana developed by MR. Shun-Te Wang

Nutritional Value of the Chlorella-Nutritional Value of the Chlorella-CryptomonadCryptomonad

ale ale ®® Product Product::Perfect health food for 21Perfect health food for 21stst century century

The first certificate of algae health food in Taiwan The first certificate of algae health food in Taiwan

Lack cell wall, easily and almost fully ( 90% ) absorbed

Perfect nutritional composition:Protein : CHO : lipid =60:14:8

Optimal combination of fatty acid: saturated FA: mono-unsaturated: polyunsaturated =

0.8:1.5:1.0

Chlorophyll: 4%Vitamin A, B, C, E

MineralsTrace elements: Zinc ( 70 mg% ), Selenium: 80mg%

Reports on the effects of CryptomonadalesReports on the effects of Cryptomonadales on medicines: on medicines: 引藻的醫療研究報告引藻的醫療研究報告

Crypto- can reduce the levels of cholesterol and low-densit

y lipoprotein （ LDL ） for up to 15-20% in one month

Regulation of blood glucose and glycosylated Hgb to nearl

y normal in 3-6 months

Crypto- can modulate immune function ( contact dermatiti

s, psoriasis, rheumatoid arthritis, and wound healing ) 。

Crypto- contains phycocyanin and has anti-viral effects

Potential prophylactic effects for cancers 。

引藻可降低自願受測者血中膽固醇引藻可降低自願受測者血中膽固醇A. Cholesterol

165

170

175

180

185

190

195

200

205

210

215

before 2 weeks 4 weeks after

chol

este

rol (

mg/

ml)

** 資料來自國衛院 蘇益仁主任

PPARs ( peroxisome Proliferator Activated Receptor ): the most promising molecules for drug development in 21th century

Peroxisome proliferator-activated rPeroxisome proliferator-activated receptor pathway diagrameceptor pathway diagram

From Wikipedia, the free encyclopedia

Biomedical effects of PPAR agonists: FattBiomedical effects of PPAR agonists: Fatty acid is the natural ligand for PPARsy acid is the natural ligand for PPARs

Effects of PPAR agonist on metabolic syndrome ( Effects of PPAR agonist on metabolic syndrome ( diabetes, obesity, hyperlipidemia, and hypertensiondiabetes, obesity, hyperlipidemia, and hypertension ) )

** 資料改編自 Science 2003;302:406

PPAR Agonist Activities in ChlorellPPAR Agonist Activities in Chlorella-a-CryptomonadalesCryptomonadales®® and Its Biomedi and Its Biomedi

cal Applicationcal Application

Ih-Jen Su, MD, PhDIh-Jen Su, MD, PhDDivision of Clinical Research, Division of Clinical Research,

National Health Research Institutes, National Health Research Institutes, TaiwanTaiwan

Extraction of PPARs-containing Fraction from Cryptomonades

** 資料來自國衛院 生技製藥組

Effects of Crypto- onPPARs expression

Tubulin

PPARα

Control Feno- GLA EPA cryp. fibrate tablet

＊ Fenofibrate ： 50 μg/ml

(as PPARα control)

＊ GLA ： γ-Linolenic Acid

＊ EPA ： Icosapentaenoic acid

＊ cryp. tablet ：引藻片＊引藻處理濃度： 2 mg/ml

0

0.5

1

1.5

2

2.5

3

3.5

1數列 1 1.25 1.21 1.19 2.87

Control Fenofibrate GLA EPA cryp. tablet

Pilot trial on psoriatic arthritis

Improvement of arthritis and skin lesions

** 資料來自成大醫學院皮膚科 李玉雲醫師 ,林旻憲醫師

曾接受數種治療方式但未獲改善之患者 , 服用引藻 10 周以上評估其關節炎狀況

0 2 4 6 8 10 12 14 16 18 20 22 240

10

20

30

40

Time (week)

PA

SI s

core

°®Å~©ÊÃö¸`ª¢¯kµh«ü¼Æ

Before 1 month 2 month 3 month0.0

2.5

5.0

7.5

10.0Before1 month2 month3 month

Time after treatment

Pai

n s

core

Crypto-PPAR induce suppression of cytCrypto-PPAR induce suppression of cytokine tumor necrosis factor-alphaokine tumor necrosis factor-alpha

Immune mdulation and anti-inflammatory effectsImmune mdulation and anti-inflammatory effects

0 10 20 30 40 50 600

100

200

300 Control

TPA 2.5ng/ml

TPA+Troglitazone

TPA+Cryp 0.25

TPA+Cryp 1

TPA ³B²z24¤p®É«á¦A¥[¤JTroglitazone,Cryp.

Time (hour)

TN

F (

pg

/ml)

Crypto-PPARs can effectively reduce viral loCrypto-PPARs can effectively reduce viral load in animal modelad in animal model

0

10000

20000

30000

40000

50000

60000

d0 d6 d12 d17 d20 d24 d27 d31 d34 d38

Time (day)

Vira

l loa

d (c

opie

s/m

l)

PBS 64403

PBS 54409

HVP 59603

HVP 66007

Cryp 8d + HVP 59901

Cryp 8d + HVP 60503

Cryp 8d + HVP 55301

HVP + Cryp 7d 59501

HVP + Cryp 7d 66503

HVP + Cryp 7d 60501

Crypto-PPAR can reduce the Crypto-PPAR can reduce the oxidative stress and genomic oxidative stress and genomic

instability: instability: anti-aging and cosmeticsanti-aging and cosmetics

Contro

lBFA

Trog

litaz

one

Feno

fibra

te

BFA +

Cryp

Cryp

BFA +

Trog

litaz

one

BFA +

Feno

fibra

te

PPAR α53KD

GRP9494KD

PPAR γ54.5KD57.6KD

eIF240KD

IRE1110KD

GADD15330KD

PERK125KD

P-JNK54KD46KD

JNK

PPARs agonists: the most promising drugs; PPARs agonists: the most promising drugs; Chlorella-Cryptomonadales the best natural Chlorella-Cryptomonadales the best natural

product in the 21product in the 21stst century century

Metabolic syndrome:Metabolic syndrome: hyperlipidemia hyperlipidemia 、 、 diabetesdiabetes 、、 hypertensionhypertension 、、 obesityobesity

Immune diseases: arhtritis, eczema, allergy Immune diseases: arhtritis, eczema, allergy viral infection: influenza, enterovirus, viral infection: influenza, enterovirus,

? HIV/AIDS , ? hepatitis? HIV/AIDS , ? hepatitis

Aging: oxidative stress, cosmeticsAging: oxidative stress, cosmetics

Cancer prevention and therapyCancer prevention and therapy

Adipocyte Differentiation AssayFor PPAR-gamma ligand

** 資料來自國衛院生技製藥組

CE 1 CE 2 CE 3 CE 4 CE 5 CE 6 CE 7 CE 8

%

0

50

100

150

200

CE 3-1 CE 3-2 CE 3-3 CE 3-4 CE 3-5 CE 3-6

%

0

20

40

60

80

100

g/ml

100 101 102

% o

f m

axim

um

0

20

40

60

80CE 1CE 2CE 3CE 4CE 5CE 6CE 7CE 8

g/ml

100 101 102

% o

f m

ax

imu

m

0

20

40

60

80CE 3-1CE 3-2CE 3-3CE 3-4CE 3-5CE 3-6

** 資料來自國衛院生技製藥組

Extracts of Cryptomonadales contain high concentration of allophycocyanin: effective fo

r anti-viral

Nature Rev Immunol. 2002; 2:748-759

Molecular mechanism of immune modulation Molecular mechanism of immune modulation and anti-inflammation by PPARsand anti-inflammation by PPARs

Index molecule in Crypto-and quality assurance

引藻片的品管檢驗 次亞麻油酸 (Linolenic Acid): 18mg/gm Free of bacteria and safety levels of heavy metals Stability in 3 months at room temperature

** 資料來自工研院