Croatia - National report

Regional Workshop 2: Improving Capacity for Diagnosis of Fish and Mollusc Diseases Banja Luka, Bosnia and Herzegovina, 20-24 October 2013,

Hotel Palas

Production data 2012

Today Croatia reaches production of 16.000 tons acuaqulture products:

4.400 tons carp and trout (šaran i pastrva), 4.000 tons tuna, 4.000 tons sea bass &sea brem (lubina i komarča)3.500 tons shellfish mussels and oysters (dagnji i

kamenica)

Fish and shellfish farms in Croatia (current status)

Movement/transfers EU – certificate - TRACES Internal market – health certificate

Importation of eggs/fry/live fish After accession, Denmark No imports from BiH!!!

Movement of live animals

Diseases recorded in the country

Viral diseases VHS

Bacterial diseases Aeromonas hydrophila, Vibrio anguillarum, Tenacibaculum

maritimum, Flavobacteruim psychrophila, Yersinia ruckeri,

Parasitic diseases Infection with Marteilia refringens Sea bass/bream – Sea lice

Fungal diseases

No records

Other health problems

No other diseases detected

Diagnostic capacity in the country

Aquatic animal diseases laboratory within country

HVI Zagreb, National reference laboratory

Description (diagnostic, research, education) VETERINARY UNIVERSITY ZAGREB INSITUTE RUĐER BOŠKOVIĆ INSTITTUE FOR OCEANOGRPAHY AND FISHERIES

Scope and species interest Fish – freshwater, marine Molluscs Crustacean

Level of diagnostic in Croatia

Level of diagnostic:Level 1: Gross observation, general necropsy

(external, internal)Level II: Bacteriology, Parasitology, Mycology,

HistologyLevel III: Cell lines, Immunological tests,

TEM, Molecular techniques

Analysis of diagnostic capacity

List of diseases and diagnostic methods used

VHS, IHN, IPN,KHV,SVC – clinical observation, detection of gross changes, histological observation, isolation of virus on cell culture, identification by ELISA, IFAT, RT-PCR, qRT-PCR, gene sequencing

Furunculosis, Vibriosis, Yersiniosis, Tenacibaculosis – Isolation of bacteria on bacteriological media, identification of genus by selective media and general properties, identification of the species by API or any other kit for biochemical identification, serological identification, PCR, sequencing.

Mollusc diseases – citology, histology, PCR, sequencing

Surveillance of nationally listed diseases

Surveillance and diagnosis are conducted only for pathogens listed in OIE and EU, on species present in Croatia

Exception ISA on trout OSHV1 – in Ostrea edulis White spot disease in crustaceans

Surveillance and monitoring of other pathogens

Targeted pathogens and aquaculture species

VHS/IHN – salmonids KHV – carp Infection with Bonamia ostreae – Oysters Infection with Marteilia refringens – Mussels,

oysters

Study of abnormal mortality

Mortality description Mortalities reported by fisherman

Mortalities recorded in 2013 – Eustrongyloides

Suspect case of KHV – put and take fisheries – ruled out

Conclusions and perspectives

Inroduction of new diagnostic tehniques – diagnostic of white spot disease, monitoring/antimicrobial resistance, further improvment of control and diagnostic tehniques in marine fish

Education of laboratory staff, proficiency test, education of governemnt officals in aquatic animal health issues