Speed: 9 knots ~ 10 days

Total distance 1000 miles or 111 hours 16 stations ( 2 hours ): 32 hours (A) 14 stations ( 4 hours ): 56 hours (B) 1 diurnal stations (D) 1 days for storms

D

CRIMEA

SEVASTOP

OL

Type Measurements per Station

(A) 10 CTD (Salpa M)

5 bathometers (microplankton)

3 Juday nets (mesoplankton)

2 BR net (macro and

ichthyoplankton)

(B) A + benthos grabs

(D) 8 series of (A)

Speed: 9 knots Total distance 1150 miles or 127 hours 24 stations ( 2 hours ): 48 hours (A) 23 stations ( 5 hours ): 115 hours (A + B) 3 diurnal stations (D) 2 days for storms

Total time: 410 hours or 18 days ~ 20 days

CRIMEA

D D

D

D

SEVASTOP

OL

EQUIPMENT – SALPA M

Technical parameters: • Weight 15 kg; • Size 110 cm; • Autonomous electric supply 24 V • Interface for connection with PC: RS 232; • Specialized program supply; • Towing speed up to 7 knots; • Submerging depth down to 200 m.

NEW PORTABLE HYDROBIOPHISICAL COMPLEX SALPA - M

Channels for measurements: • bioluminescence ( 10-13 -19-8 vt.cm-2. l-1) • temperature ( from -2 to 35 C) • pressure (range of measurments 0 - 2 Мpa); • turbidity (0,2-50); • photosynthetic active radiation (0,002 -2000

µЕ/м2*sec); • chlorophill concentration – а (0,02-100 mg/l); • electric conductivity (0,1-2 Sm/m);

Work per 1 Station: 10 sounding (0 – bottom or 0 – 150 m) Time: 20 min

(A)

EQUIPMENT – MARK III

This is an integrated instrument package comprising a Neil Brown Instrument Systems Mark III Conductivity, Temperature, Depth (CTD) profiler unit with 10 bathometers (10 l).

CTD NEIL BROWN MARK III

Samples: • Hydrochemistry • Bacterioplankton • Phytoplankton • Microplankton

Work per 1 Station: 1 sounding (usually with 5 samples: ~ 0, 10, 25, 50 and 100 m). Time: 30 min

(A)

EQUIPMENT – JUDAY NET

JUDAY PLANKTON NET

Technical parameters: • Diameter 37 cm • Mouth area 0,1 m2; • Mesh size 100 µm

Samples: • Meroplankton • Mesoplankton

Work per 1 Station: 1 haul in the upper mixed layer (~0-15 m); 1 haul in thermocline (~15-30 m); 1 haul below thermocline (~30-150 m); Total time: 40 min

(A)

EQUIPMENT – BR NET

BOGOROV – RASS PLANKTON NET

Technical parameters: • Diameter 80 cm • Mouth area 0,5 m2; • Mesh size 300 µm

Samples: • Ichthyoplankton • Macroplankton

Work per 1 Station: 1 haul (0-50 m); 1 haul (0-150 m); Time: 20 min

(A)

(B)

“OCEAN 50” GRAB

Technical parameters: • Mouth area 0,25 m2; • Weight 166 kg; • Width 504 mm; • Length 638 mm.

Work per 1 Station: 2 samples from sea bed Total time: 2 hours

EQUIPMENT – BENTHOS GRABS

1. “SALPA M” (10 SOUNDS, ~ 0-100 m)

2. MARK III BATHOMETER (1 SOUND, 5 SAMPLES ~ (0, 10, 25, 50, 100 m)

3. JUDAY PLANKTON NET (3 NETS)

~ (0-15; 15-25;25 – 150 m).

4. BR PLANKTON NET (2 NETS)

~ (0-50; 50 – 150 m)

DUIRNAL STATIONS

8 SERIES OF (A) DURING 24 HOURS:

(I)

Type Measurements per Station

(I) Hydrology (CTD)

Phytoplankton (Bathometer)

Mesoplankton (1 Juday net)

Macroplankton (1 BR net)

Ichthyoplankton (1 BR net or Bongo net)

(II) (I) + Bacterioplankton

(III) (I) + Turbidity (Salpa M)

PAR (Salpa M)

ATP of microplankton

(I)

(II) (I)

(I)

(I)

(I)

(I)

(III)

(I)

(II-III)

EQUIPMENT CTD – SALPA M

Technical parameters: • Weight 15 kg; • Size 110 cm; • Autonomous electric supply 24 V • Interface for connection with PC: RS 232; • Specialized program supply; • Towing speed up to 7 knots; • Submerging depth down to 200 m.

NEW PORTABLE HYDROBIOPHISICAL COMPLEX SALPA - M

Channels for measurements: • bioluminescence ( 10-13 -19-8 vt.cm-2. l-1) • temperature ( from -2 to 35 C) • pressure (range of measurments 0 - 2 Мpa); • turbidity (0,2-50); • photosynthetic active radiation (0,002 -2000

µЕ/м2*sec); • chlorophill concentration – а (0,02-100 mg/l); • electric conductivity (0,1-2 Sm/m);

WORK PER 1 STATION: 2 SOUNDING (0 – 40, 0 – SEA BED) TIME: 20 MIN

(A)

EQUIPMENT CTD – VALEPORT PROFILER

The Monitor CTD has been developed from Valeport's Midas CTD, utilizing the same accurate, robust sensors and synchronized sampling technique, but packaged as a smaller, lightweight unit to suit small boat or shallow water applications. The instrument will operate autonomously, with setup and data extraction performed by direct communications with PC before and after deployment. It also operates in real time, with a choice of communication protocols for a variety of cable lengths, all fitted as standard and selected by pin choice on the output connector

VALEPORT MONITOR CONDUCTIVITY TEMPERATURE & DEPTH PROFILER (CTD)

Work per 1 Station: 2 SOUNDING (0 – 40 OR 0 – SEA BED) TIME: 20 MIN

EQUIPMENT – BATHOMETER (10 L)

BATHOMETER FROM CTD NEIL BROWN MARK III

Samples for: • Hydrochemistry • Bacterioplankton • Phytoplankton • Microplankton

WORK PER 1 STATION: 2 SOUNDING ( ~ 0 AND 25 M). TIME: 30 MIN

EQUIPMENT – BR NET

JUDAY PLANKTON NET

Technical parameters: • Diameter 37 cm • Mouth area 0,1 m2; • Mesh size 100 µm

Samples: • Meroplankton • Mesoplankton

Work per 1 Station: 1 HAUL (~0 - SEA BED); OR 1 HAUL (~0 – 40 M);

EQUIPMENT – BR NET

BOGOROV – RASS PLANKTON NET

Technical parameters: • Diameter 80 cm • Mouth area 0,5 m2; • Mesh size 300 µm

Samples: • Ichthyoplankton • Macroplankton

Work per 1 Station: 1 HAUL (~0 - SEA BED); OR 1 HAUL (~0 – 40 M);

EQUIPMENT – BONGO NET

BONGO PLANKTON NET

Technical parameters: • Diameter of each: 37 cm • Mouth area each: 0,1 m2; • Mesh size: 100 µm and 300 µm

Samples: • Macroplankton • Ichthyoplankton

Work per 1 Station: 1 HAUL (~0 – 40 M);

Ichthyological monitoring, which includes the control of the

species composition of the whole fish fauna of the Black

Sea in most countries is not conducted. Not surprisingly,

in the annual reports on the biodiversity of the Black Sea

Commission, these data are not available. The importance

and need for these studies is obvious. For more than 15

years in the IBSS we hold constant study of the species

composition of fish, biological condition, the

characteristics of the distribution of fish mainly in the

coastal zone and on the shelf of the Crimea. It should

immediately be noted that the application of a uniform

methodology for the collection of ichthyological material is

not possible. In our studies, we use the following methods

of fishing.

IBSS Ichthyological monitoring

Ichthyological monitoring

Traditionally, for over 50 years in the IBSS for collection of

ichthyological material used bottom small commercial

trapnets. They are exposed to some of the bays of

Sevastopol. Fishing provides a specialized team of

fishermen IBSS 1 once a week throughout the year.

Ichthyological monitoring

Ichthyological monitoring

Ichthyological monitoring As in the bays of Sevastopol, and in other

coastal areas of the Crimea fishing carry towed

shrimp sack having an inlet semicircular size

1.6 0.8 m, an area of 1 m2, equipped with a

net of mesh size 6.5 mm.

Ichthyological monitoring

Ichthyological monitoring For over 10 years, we regularly get ichthyological material from volunteers

underwater hunters. In June 2014 between IBSS and the Association of submarine

hunters and divers of Sevastopol and Crimea signed an agreement on scientific and

technical cooperation, which provides for the collection of information underwater

hunters specially developed questionnaire and the ability and desire to provide

samples of fish.

Ichthyological monitoring

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1. Вид

2. Дата сбора (год, месяц, число)

3. Место сбора

4. Широта N

5. Долгота E

6. Количество особей

7. Тотальная длина , мм

8. Стандартная длина, мм

9. Длина по Смиту, мм

10. Масса, г

11. Пол

12. Стадия зрелости

13. Возраст

14. Плодовитость

15. Ожирение

16. Наполнение желудка

17. Орудие лова

18. Глубина, м

19. Температура воды, °С

20. Фиксатор

21. Дата анализа

22. Примечания

Ichthyologycal analysis of 26 500 exemplars of adult fish belonging to

150 species has been conducted during 2005 - 2014

22 parameters are given in the database

Proposals. Within the framework of the Black Sea

Commission working group on biological diversity to

organize the collection of information on species diversity

of fish, biological and structural characteristics of both

populations of individual species and ichthiocenos of

different regions of the Black Sea.

Ichthyological collection of material can be carried out by

all available for the purpose of fishing gear in accordance

with the rules of scientific gear for a particular country,

but the results ichthyological monitoring must be

submitted on a unified scheme, which provides for the

establishment and regular updating Ichthyologic

database.

My colleagues and I are ready to take part in an

ichthyological monitoring in the region of the Crimea as

part of cooperation with the Black Sea Commission and

the program EMBLAS 2.