creative biogene sophisticated gene synthesis & cloning is easy at creative biogene
DESCRIPTION
At Creative Biogene, we can synthesize and clone any DNA sequences, even complex, GC-rich, repeated, or long genes, into prokaryotic, eukaryotic or viral vectors.TRANSCRIPT
Sophisticated Gene Synthesis & Cloning Is Easy at Creative Biogene
The Traditional Cloning
• For a traditional cloning process, it is generally thought to be lengthy and complex for eight reasons, as follows:
• Cloning strategy design• Primer Synthesis• PCR and restriction enzyme digestion• Gel extraction and ligation• Transformation and plasmid miniprep• Screen by enzymatic digestion• Sequencing reaction preparation• Sequence assembly analysis
Challenges• Either to solve those issues or skip to the
next step, the process can still be challenge due to factors, such as a physical template, minor or major changes, intensive labor, unpredictable results, etc. All those procedures can drain your reagents and precious time that supposed to be used in more refined points.
Cloning at Creative Biogene
• At Creative Biogene, we can synthesize and clone any DNA sequences, even complex, GC-rich, repeated, or long genes, into prokaryotic, eukaryotic or viral vectors.
• Creative Biogene has accomplished hundreds of thousands of gene-synthesis & cloning projects for scientists over the world.
• Creative Biogene’s gene synthesis and Cloning service is flexible to accommodate projects from single gene synthesis to whole genome synthesis.
Creative Biogene’s Gene Clone Service includes:
•Codon Optimization for any given expression system.• Synthesis of genes of interest with or without tags, and cloning the target sequence into a standard vector.•Sub-cloning of your synthetic gene products into a vector of your choice.•Amplification of specific cDNA by PCR/RT-PCR and cloning into a standard vector or a vector of your choice.
Other services provided by Creative Biogene:
•Custom Viral Service • RNAi Service • Gene Service • Aptamer Service • Plasmid DNA Production Service • Stable Cell Line Generation • Oligonucleotide Synthesis Service
Contact
• USA45-1 Ramsey Road, Shirley, NY 11967, USATel: 1-631-626-9181Email： [email protected]
• United Kingdom St John's Innovation Centre, Cowley Road, Cambridge CB4 0WS, UK Tel: 44-207-048-3343 Email： [email protected]
• Web: http://www.creative-biogene.com/Services/Gene-Service/Gene-Synthesis-and-cloning-service.html