crc tissue core fish initiative/cytogenetics team mayo:osu: dan van dykenyla heerema neil kayjohn...
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CRC Tissue CoreFISH initiative/Cytogenetics Team
Mayo: OSU:Dan Van Dyke Nyla HeeremaNeil Kay John ByrdStephanie Smoley Carlo Croce (P1)Jeanette Passow (Biostats)
DF : LIJ:Paola Dal Cin Prasad KoduruJennifer Brown Kanti RaiDonna Neuberg (Biostats) MDA:Lillian Werner (Biostats) Bill Wierda
UCSD: Tom KippsMarie Dell’AquilaLaura Rassenti (TC)Andrew Greaves (Core A Bioinformatics)Monica Cook (TC)
CRC Tissue CoreFISH initiativeFunded in 2005
CRC FISH data associated with CLL samples
May 04= 2% of CRC pts had FISH dataMay 09= 64% of CRC pts have FISH data32 fold increase
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CRC Tissue Core FISH initiative funded in 2005
2000(start of TC
sample collection)
2005(Record FISH entry into DB
Uniform probes, SOPs )
May 1, 2009
1008 pts with recorded FISH
2226 pts with recorded FISH
CRC Tissue CoreFISH initiativeFunded in 2005
Total # of pts in CRC= 5055Total # of CRC pts with FISH entries = 3234/5055 (64%)Total # of pts with FISH entries that have serial FISH entries: (longitudinal studies) = 582/3234 (18%)
% of patients/site with FISH entries pre and post 2005
CRC Tissue CoreFISH initiativeFunded in 2005
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Summary of patients FISH entries dates in relation to the respective date of Dx
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yrs from Dx to FISH sample date
# of pts with FISH entry
CRC Tissue Core FISH/Cytogenetics
1) Data Entry: standardized SOP (Mayo QA/QC)archived smpls (pre & post 05)/new samples/monthly updates
2) Data retrieval : (for hypothesis driven studies)Biostats Core B (Donna,Jeanette,Jillian) : QA/QC
3) Multiple error checks gives us high confidence in the quality of the data (standardized scoring SOPs among all CRC sites)
4) FISH data associated with serial samples/pt (longitudinal studies, clonal evolution) FISH data associated with same date of TC sample
5) CRC FISH projects : Dan will present next
Many thanks!
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CRC FISH CpG experimentsSummary
CRC FISH CpG Studies Summary 5 mls of fresh blood sent out to each site4/1/09Laura Rassenti CRC Tissue [email protected]
Experiment # date sent samples samples ID sample date FISH sites results#1 Aug 19, 2008 TJK0827 8/19/08 Mayo, UCSD,OSU,LIJ,DF done
TJK0473 8/19/08TJK0589 8/19/08
#2 Sept 23, 2008 P1 (normal) 9/23/08 Mayo, UCSD,OSU,LIJ,DF doneP2 (TJK0502) 9/23/08
#3 January 20, 2009 P3 (TJK0228) 1/20/09 Mayo, UCSD,OSU,LIJ,DF doneP4 (MJK0617) 1/20/09
Please analyze at least 20 metaphase cells band-to-band. Karyotype one normal cell (if present), any clonal abnormality, and every non-clonal abnormal cell.If there is a non-clonal cell please proceed to 30 metaphase cells band-to-band.
Send a written report of results and copies of karyotypes to Dan for summarization .
Contacts at Mayo: Dan Van Dyke [email protected] 507-284-6776Stephanie Smoley [email protected] 507-284-8338