CPCSEA

CPCSEA GUIDELINEFOR LABORATORY

ANIMAL

•

Submitted toDr.Nagraj sirHod of dept Pharmaceutical Analysis

Presented by Abhishek Mondal1st MpharmDept of pharmacology

Contents:• INTRODUCTION• OBJECTIVES • VETERINARY CARE• ANIMAL PROCUREMENT • QURANTINE , STABILIZATION , SEPARATION• TRANSPORT OF ANIMAL• LABORATORY ANIMAL HUSBANDARY & MANGE MENT • ANAESTHESIA AND EUTHANASIA

WHAT IS CPCSEA Committee for the purpose of control and supervision of

experiments on animals. CORE MEMBER:

Hon. Smt. Maneka Gandhi – drafted under chairperson, CPCSEA

Mr. A.K.Joshi – Member Secretary, CPCSEA

SUBCOMMITTEE MEMBERS:Dr. Manju Sharma – secretary, department of biotechnology

MembersDr. vasanth muthuswamy- Sr. DDG, Indian council of medical

researchDr. Lal Krishna – ADG,(AH) ICAR, New Delhi

Dr. S.S. Murugan – SGS India private limited, Chennai

FUNCTION OF CPCSEA Registration of establishments conducting animal

experimentation or breeding of animals for this purpose. Selection and appointment of nominees in the Institutional

Animal Ethics Committees of registered establishments. Approval of Animal House Facilities on the basis of reports of

inspections conducted by CPCSEA. Permission for conducting experiments involving use of

animals. Recommendation for import of animals for use in

experiments. Action against establishments in case of violation of any legal norm/stipulation.

WHY ANIMALS ARE USED IN RESEARCH

Reasons The principle of anatomy and physiology are true for human

and , especially mammals . Certain strains or breeds of animals get the same disease or

conditions as humans. With animals we can control their environment(temperature ,

humidity, etc) and shield them from disease or condition not related to the research ( control their health).

We can use scientifically – valid no of animals.

Objectives • The goal of these guidelines is to promote the

human care of animal used in biomedical and behavioural research and testing.

• To avoid unnecessary pain before ,during and after experiment .

• To provide guideline for -housing ,care ,breeding and maintenance -source of experimentals animals -acceptables experimentals procedures for anesthesia and euthanasia

Veterinary care o Adequate veterinary care must be provided and

is the responsibility of a veterinarian or a person who has training or experience 'in laboratory animal sciences and medicine.

o Observed regularly for sign of illness, injury, or abnormal behavior

o Contagious disease – isolated from healthy animal.

Animal procurement• All animals must be acquired lawfully as per the

CPCSEA guidelines.• A health surveillance program for screening incoming

animals should be carried out to assess animal quality.• Methods of transportation should also be taken into

account. • Each consignment of animals should be inspected for

compliance with procurement specifications, and the animals should be quarantined and stabilized according to procedures appropriate for the species and circumstances.

QURANTINE ,STABILIZATIONSEPARATION

QUARANTINE • Separation of newly received animals from those

already in the facility until the health and possibly the microbial status of newly received animal have been determine.

• A minimum duration of quarantine for small animal-1 week and for larger animal-6 week.

• An effective quarantine minimizes the chance for introduction of pathogens into an established colony.

STABILIZATION AND SAPARATION

STABILIZATION- Physiologic, psychological and nutritional

stabilization should be given before their use. Duration of stabilization will depend on type and

duration of animal transportation , and species of animal.

SEPARATION- Physical separation of animal by species is

recommended to prevent interspecies disease transmission and to eliminate anxiety and possible physiological and behavioural changes due to interspecies conflict.

It shall be acceptable to house different species in the same room ,e.g. two species have a similar pathogen status and are behaviourally compatible.

SURVELLANCE, DIAGNOSIS, TRETMENT AND CONTROL

DISEASE

• All animal should be observed for signs of illness, injury, or abnormal behaviour by animal house staff.

• Animals that show signs of a contagious disease should be isolated from healthy animals in the colony.

PERSONAL HYGIENE• Animal care staff maintain a high standard of

personal cleanliness. • Clothing suitable for use in the animal facility .• It acceptable to use disposable gear such as

gloves, masks, head covers, coats, coveralls and shoe covers.

• Person should change clothing as often as is necessary to maintain personal hygiene.

• Personnel should not be permitted to eat, drink, smoke or apply cosmetic in animal rooms.

TRANSPORT OF LABORATORY ANIMALS

• The main considerations for transport of animals are

• the mode of transport, • the containers, the animal density in cages, food

and water during transit, • protection from transit infection, injuries and

stress.

LABORATORY ANIMAL

• HUSBANDARY & MANAGEMENT

PHYSICAL FACILITIES• BUILDING MATERIALS- moisture-proof, fire-resistant,

seamless materials are most desirable for interior surfaces including vermin and pest resistance.

• CORRIDOR- wide enough to facilitate the movement of personnel as well as equipments and should be kept clean.

• UTILITIES- water lines, drain pipes and electrical connection

• ANIMAL ROOM DOORS- rust, vermin and dust proof. it properly within their frames and provided with an observation window.

• FLOORS- smooth, moisture proof, non-absorbent, skid-proof,.

DRAIN

S floor drains are not essential in all rooms used exclusively for housing rodents.• WALLS & CEILINGS- free of cracks, unsealed utility penetrations, or imperfect junction with doors, ceilings, floors and corners.• STORAGE AREAS- separate storage areas should be

designed for feed, bedding, cages and materials not in use.

• FACILITIES FOR SANITIZING EQUIPMET AND SUPPLIES- an area for sanitizing cages and ancillary equipment is essential with adequate water supply.

• EXPERIMENTAL AREA- should be carried out in a separate area from the place where animals are housed.

ENVIRONMENT• Air conditioning is for laboratory animals.

temperature with in the range of 180 -290c

relative humidity- 30-70% throughout the year

• for large animal comfortable zone-18-37°˚c

POWER & LIGHTING-

the electrical system should be safe and provide appropriate lighting and a sufficient no. of power outlets.

A time control light system should be used.

NOISE CONTROL- noise free environment

ANIMAL HUSBANDARY• CAGING & HOUSING SYSTEM- Adequate ventilation Meet the biological need of animal Keep the animal dry and clean

Cages made of steel or painted steel Feeding and watering devices should be easily

accessible for filing, changing, cleaning and servicing.

FOOD

• Should be fed palatable, non-contaminated and nutritionally adequate food.

• Diet should be free from heavy metals. BEDDING Absorbent, free of toxic chemicals or other

substances that could injure animals or personnel Should be removed and replaced with fresh

materials as often as necessary to keep animal clean and dry.

WATER• Ordinarily animals should have continuous access

to fresh, potable, uncontaminated• drinking water, according to their particular

requirements. SANITATION CLEANLIESS• Sanitation is essential in an animal facility. Animal

rooms, corridors, storage spaces, and• other areas should be cleaned with appropriate

detergents and disinfectant .

Waste disposal• Wastes should be removed regularly and

frequently. All waste should be collected and• disposed in a -safe and sanitary manner. The

most preferred method of waste disposal is incineration.

EMERGENCY, WEEKEND AND HOLIDAY CARE• Animal should be cared for by qualified personnel

every day, including weekends and holidays, to safeguards their well- being including emergency veterinary care

Record keeping The Animal House should maintain following records:1. Animal House plans, which includes typical floor plan, all

fixtures etc.2. Animal House staff record - both technical and non - technical3. Health record of staff! animals.4. All SOPs relevant to the animals5. Breeding, stock, purchase and sales records6. Minutes of institute Animals Ethics Committee Meetings7. Records of experiments conducted with the number of

animals used (copy of Form b)8. Death Record9. Clinical record of sick animals .10. Training record of staff involved

ANAESTHESIA

EUTHANASIAANAESTHESIA• It must also be ensured that the anesthesia is

given for the full duration of experiment.• and at no stage the animal is conscious to

perceive pain during the experiment.• sedatives, analgesics and anaesthetics should be

used to control pain or distress under experiment

Euthanasia

(eu =good.thanatos =death) PURPOSE • End of experiment , to provide tissue for

scientific purpose.• Free the animal of pain • Diseased animal or animal in bad condition

Methods

• Physical methods • electrocution , exanguination , decaptation ,

cervical dislocation • Drug administration overdose of chloral hydrate, ketamine , chloroform

References • icmr.nic.in/bioethics/final• cpcsea.nic.in• www.aaalac.org/resources/SOP• Google.com

Thank you