coulochem iii electrochemical detector · 2019. 3. 11. · sensor 1 thus eliminating it from...

SensitivityReliability

ConvenienceFlexibility

Coulochem IIIElectrochemical Detector

CoulochemBrochureRevE_FINAL:Copy of 5300 BRO MECH REVD.qxd 12/23/09 10:16 AM Page 2

Principle of Coulometric Technology:Compound A is completely reacted atSensor 1 thus eliminating it from detectionat Sensor 2. Compound B passes Sensor 1unchanged and is detected at Sensor 2.The result is that A and B are resolvedelectrochemically.

THE DEMAND FOR H I GHLY SEN S I T I V E AND

SELECT I V E DETECT I ON I N HPLC HAS NEVER BEEN

GREATER . ELECTROCHEM I C A L DETECT I ON I S

THE AN SWER WHEN YOU NEED TO MEA SURE P I COGRAM TO

FEMTOGRAM LEVEL S OF OX I D I Z A B LE

OR REDUC I B L E COMPOUND S

The ESA Coulochem® III detector is the standard inhigh sensitivity electrochemical detection. With its modular

design, the Coulochem III platform can be used with anyHPLC system. All new electronics with the latest in digitaltechnology guarantees the lowest detection limits possible.

The Coulochem III incorporates DC and Pulse/Scan modesin discreet modules. By optimizing the detector for the low

signal in DC, the detector achieves the highest possiblesensitivity from capillary to normal bore chromatography.

Pulse mode supports variable timing of potentials and durationof waveforms to optimize the application. Scan mode helps

select the optimal potentials. The Coulochem III detectoroffers DC, Pulse/Scan or both.

O F ALL THE HPLC DETECTOR S , NONE ARE A S SEN S I T I V E

AND SELECT I V E W I TH A S W IDE A DYNAM I C RANGE A S AN

ELECTROCHEM I C A L DETECTOR FROM ESA

.

.

PerformanceTODAY,FORWARD THINKING


For DC mode, each Coulochem III detector comes withtwo analytical channels and an additional channel, uniqueto ESA, for mobile phase cleaning or sample pretreatment.

In Pulse mode, you can select potential and duration of up to4 segments to give the ultimate in stability and reproducibility.

Operate switching valves or signal for external events, change gainranges or potentials with Time Line or shut off cells at the end

of a run. All this stored as one of 25 methods for easy recall.

All standard ESA cell designs can be used with theCoulochem III detector platform. Expanded gain ranges make itready for any cell type. Enhanced processor speed provides dataacquisition fully compatible with today’s faster chromatography.

Sensitivity

“The ESA Coulochem is by far the mostsensitive EC detector on the market today,very reliable and backed by superiortechnical support. Its sensitivity makesit stand out from the others.”

- Professor Timothy MaherMassachusetts College of Pharmacyand Health Sciences

With the sensitivityand selectivity of theCoulochem III, peerinto the living brain,view the mechanismsof aging and canceror create a pictureof health and disease.


State-of-the-industry electronics guarantee reliability. The provenCoulometric cell design is well known for ruggedness and operating every

time right out of the box. The solid state reference electrode used in all ESAcells never needs replacing, refilling or any other maintenance.

Hands off, fully automated operation is a reality.

MORE TO RELIABILITY THAN JUST HARDWARE.

ESA has been making electrochemical detectors for over 40 years. Thename ESA is synonymous with reliable electrochemical detection in HPLC.The Coulochem III is backed by superior instrument service and superlativeapplications support by experts that specialize in ECD. ESA electrochemicaldetectors are used in the most demanding environments imaginable. From

nuclear reactors to clinical laboratories – the Coulometric family ofdetectors have proven their reliability.

ReliabilityTHE IMPACT OF

RELIABILITY IS ONE

OF THE KEY DESIGN

ELEMENTS OF THE

COULOCHEM III


The advanced features of theCoulochem III platform provideperformance and reliability in a

detector that is convenient to use.The Coulochem III detector was

designed by people that useECD every day.

SOFTKEYS

menus that areeasy to navigate

LARGE DISPLAY

easier to readwith moreuseful information

OPTIONAL

INTEGRATED ORGANIZER

protects columnsand cells, with or with-out temperature control

MODULAR BOARDS

a choice of modes:DC, Pulse/Scan orboth

ADVANCED ELECTRONICS

reduced noise

A CHOICE OF CELLS

current and future

LARGE KEY PAD

easy to see,easy to use

Convenience


The Coulochem III detector comes with achoice of cell designs. From simple analysis

with amperometric electrodes to sophisticatedsample manipulation with Coulometric cells,

ESA offers the flexibility to save time andeffort in optimizing analytical conditions.All ESA cells come standard with a solid

state, maintenance free reference electrodeknown world wide for its reliability.

PRE-ANALYTICAL CELLS – The highconversion efficiency of the coulometric

electrode when used before the analytical cellprovides an additional level of selectivity.

When placed before the injector, mobile phasecan be electrochemically cleaned for lowerbackground and enhanced performance.

SPECIALTY CELLS – Some applicationshave unique requirements. The 5014B and

5041 cells are specifically designed and testedfor the demands of microdialysis in

neuroscience where extreme sensitivity isrequired and high sample salt content is

encountered.

SYNTHESIS CELLS – Electrochemicaloxidation can be used to produce drugmetabolites in quantities suitable for

MS, NMR and biological studies.

COULOMETRIC CELLS – There is noelectrochemical cell more sensitive or reliable. Period.

Proven in thousands of laboratories worldwide, theflow-through design with high surface area and

minimized diffusion distance provides 100% analyteconversion and the highest signal possible. Each cellhas two working electrodes for maximum analytical

flexibility using modes pioneered by ESA’sApplications Laboratory. Screen and Redox modesincrease selectivity and reduce sample preparation.

Channel ratioing gives you added assurance of peakidentity. When downtime is unacceptable, ESA

Coulometric cells are the only answer.

AMPEROMETRIC CELLS – These singleamperometric electrodes are routinely used for

capillary or microbore chromatography, in pulsemode or when metal electrodes are required. This

design is used with the ESA Boron Doped Diamondelectrode which expands the useable potentials range

in electrochemical detection.

THE ENDLESS POSSIBILITIES OF

FlexibilityA CHOICE OF CELLS FOR OPTIMIZATION OF YOUR ANALYSIS


The Coulochem III detector is a platform forthe future. It is designed so that you can recon-figure the instrument now or in the future to fityour analytical needs.

The organizer module houses multiplecolumns, Analytical and Guard cells and pulsedampers. It conveniently mounts a manualinjector. Once inside, cells andcolumns are protected fromthe environment. Theorganizer can be easi-ly removed oradded withouttools. A completeanalytical config-uration can beneatly assembledand stored for aquick and easychange of method.

OPERATE EXTERNAL DEVICES

The Coulochem III detector allows operation ofexternal switching valves for on-line samplepreparation, column switching or on-linemicrodialysis. The power of the Coulochem IIIpermits fully automated analyses. Methods canbe easily recalled for later use. With Time Line

you can program gain changes, run Clean Cellor turn off pumps at the end of a method.

DC OR PULSE: YOU CHOOSEWITHOUT COMPROMISE

Other Electrochemicaldetector designsincorporate thehigh sensitivityDC and pulsecomponents on thesame boards. Thismeans that theelectronics are

compromised for thediffering requirements

of these disparatemodes of operation. In

the CoulochemIII there is nocompromise. DC and pulse

are on separate boards, opti-mizing the electronics for each

mode.When DC or Pulse mode is needed, simply addthe specific board for a fully optimized detector.Now there is no requirement to purchasefunctions that will not be used. TheCoulochem III detector offers flexibility in bothexperimental and instrument design, ensuringthat it will never become obsolete.

THAT MEANS YOUR DETECTOR WILL NEVER BECOME OBSOLETE

THE COULOCHEM III GIVES YOU FLEXIBILITYIN BOTH EXPERIMENTAL AND INSTRUMENT DESIGN


PLASMA CATECHOLAMINES

High selectivity along withsensitivity can be achievedthrough the dual electrodesof the ESA Coulometric cells.In the analysis of the plasmacatecholamines all thecomponents are oxidized atthe first electrode and thenmeasured reductively at thesecond electrode. In this waymany common interferencesare eliminated. This is onlypossible with the highefficiency of the coulometricelectrode.

ENHANCED SELECTIVITYWITH REDOX MODE

Applications

NEUROTRANSMITTERS

With the wide choice of cellsavailable the Coulochem IIIdetector can be optimized forany sample or application. Formicrodialysis in neuroscience,extensive research by the ESAApplications Laboratory hasdetermined that the idealconfiguration for sensitivityand reliability is the Model5041 cell with a microborecolumn.

OXIDATIVE STRESS MARKERS

Using the dual electrodecapability of the Coulochem IIIdetector you can “tune” intospecific analytes. With theguanine adducts you canmeasure both guanine and theoxidative adduct simultaneously,without the vagaries added withmultiple detectors.

SENSITIVITY, RELIABILITY, CONVENIENCE AND FLEXIBILITY ALLCOME TOGETHER TO SET A NEW STANDARD IN PERFORMANCE

MAXIMIZING RELIABILITYAND SENSITIVITY

FLEXIBILITY

Time (minutes)

Extracted Plasma Sample 20 nA gain

3MGGua

Guo

7MG

2dG

O6M

G

Channel 2 (E2) 8OH2’dG

Channel 1 (E1)

Basal Frontal Cortical MicrodialysisPerfusate Sample 2 nA gain


THIOLS

Problem analysis, such as thethiol glutathione, is madereliable with the Boron-DopedDiamond (BDD) electrode.This electrode has a widepotential window, even withaqueous mobile phases, thuswidening the range of mole-cules addressed by ECD. This,along with the low fouling andresilience of the BDD electrodefacilitates the use of ECD formany difficult analytes.

EXPANDED RANGE OFANALYTES

SYNTHESIS

The electrochemical redoxprocess is related to biolgoicalredox and drug metabolism.Synthesize authentic drugmetabolites in hours, notmonths, in quantities suitablefor NMR, MS or biologicalstudies. Connect on-line withMS to access metabolicproducts or stability. Gooff-line to collect metabolitesin microgram quantities.Produce intermediates tosimplify batch synthesis.

MORE THAN HPLC EC-MS

PhytoestrogensFood/BeverageCatecholaminesHomocysteineAntioxidantsNutraceuticalsHerbicidesErythromycinMorphinePhenolsAntibiotics

OctopamineCompoundNanoSynthesis

MonoaminesTea CatechinsAdduct FormationSerotoninGABA/GlutamateCatecholestrogensTocopherolPharmacokinetics

ApomorphinePolyaromaticHydrocarbons

Lipoic acidXenoestrogensHydroxyl FreeRadicals

Amino AcidsBisphenolCapsaicinProanthocyanidins

ErgotamineHaloperidolAscorbic AcidPhenothiazinesAcetaminophenTricyclicAntidepressants

Oxidative StressMarkers

Acetylcholine/CholineThiols

ASSISTED IONIZATION

React “problem” compounds toenable or enhance detectabilityin MS or LC-MS systems.Identify parent compoundsand reaction products ormetabolites. Provide additionalcapabilities to complement MSor LC-MS operations.

FlavonoidsNeurotransmittersBHT/BHAPharmaceuticalsGlutathioneMetabolismROS/RNSGuanine AdductsCarbohydrates3-NitrotyrosineVitamins

Thiols, disulfides and thiothers


ESA - A Dionex Company is ISO 9001 and ISO 13485 certified. Specifications subject tochange without notice. ESA and Coulochem are registered trademarks of the DionexCorporation. The Coulochem detector is covered by multiple patents. All other trademarks areproperty of their respective owners.

ESA - A Dionex Corporation22 Alpha RoadChelmsford, MA 01824-4171 USA+1 978.250.7000 Phone+1 978.250.7090 Fax+1 800.959.5095 US Toll freeEmail: [email protected]

Corporate HeadquartersDionex Corporation1228 Titan WaySunnyvale, CA 94088-3603+1 408.737.0700 Phone+1 408.730.9403 Faxwww.dionex.com

70-5445Rev E

Specifications and Certifications

Detector Configuration: DC potentiostat for 1 or 2 cells and Potentiostat for Guard Cell and/or Scan Mode/Pulse Mode

Operating Modes: DC, Pulse, Scan (Time Line method can be attached to DC and Pulse methods)

Potential Range: ± 2000 mV in 1 mV steps

Full Scale Current Range: 10 pA to 1 mA in 1-2-5 sequence (DC Mode)

Filter Time Constants: 0.2 to 10 seconds in 1-2-5 sequence (DC mode)

Typical Noise: < 750 fA (0.75 pA) with a 500 MΩ, 0.47 µF test load and a 2 second filter (DC Mode)

Signal Output: ± 100 mV, ± 1 V

Output Resolution: 24 bit bipolar

Guard Cell Potential: ± 2000 mV in 1 mV steps

Event Marker: Triggered by front panel keypad, timed operation or RS-232C control

Autozero: Triggered by front panel keypad, rear panel contact closures, timed operation or RS-232C control

Function Keys: Autozero, Event Marker, Cell On/Off, Run/Stop

RS-232C Interface Capability: Full parametric instrument control of DC and Pulse

Method Storage: Up to 25 methods (any combination of DC, Scan, Pulse and Time Line)

Power: 100-240 V AC, 50/60 Hz, 24 VA

Detector Dimensions: 19.5” (L) x 9” (W) x 11.25” (H), (49.5 cm x 23 cm x 28.6 cm)

Detector Weight: 14.8 lbs (6.7 kg) Detector only with DC and Pulse/Scan

Certifications: UL, CUL and CE

Other: Active input for cell protection

Time Line Specifications (DC and Pulse Mode)

Programmable Changes: Selectable per channel at times from 0 to 9999.99 minutes in 0.01 minute increments

Autozero, Event Mark: Independently selectable at start of program and with each current range change

Program Start: Via front panel keypad or rear panel contact closure

External Device Control: Control of up to 5 external devices

Program Control: Current Range, Autozero, Event Mark, Potential, Filter Constant

Program Repeats: 1 to 9999

Automated CV Curve: Start and Stop Potential, Potential Increment for automating determination of optimal operating potential

About ESA - A Dionex Company

For more than 40 years, ESA Biosciences has been dedicated to developing solutions for bioanalytical detection – a resolve that has resultedin many US and foreign patents for analytical and diagnostic devices. We are a full-service, ISO-certified and FDA-registered supplier ofinstruments, reagents, and turnkey detection systems. Whatever the level of need, you can count on ESA for support – from help in makingthe best choice for your application, through installation, training, and post-sale service and support.

For more information, visit: www.esainc.com


coulochem iii electrochemical detector · 2019. 3. 11. · sensor 1 thus eliminating it from...

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