cosmetic sodium hyaluronate
TRANSCRIPT
KUMAR ORGANIC PRODUCTS LIMITED
Ingredients for us
BIO/002/2012-13Dr. Lakshmi Sridhar
18th Sep 2012
SODIUM HYALURONATE
(KOPURON)
Cosmetic grade
BIO/002/2012-13Dr. Lakshmi Sridhar
18th Sep 2012
Sodium Hyaluronate - Identification
BIO/002/2012-13Dr. Lakshmi Sridhar
18th Sep 2012
• INCI name: Sodium hyaluronate
• CAS No. 9057-32-7
• Product name: Poly-(sodium-b-D-glucuronate-[1-3}- N-acetyl-D-glucosamine-[1-4]
•Other names: Hyaluronic acid, Hyaluron, Hyaluronan
• EINECS/ELNICS: 232-678-0
Sodium Hyaluronate - Structure
BIO/002/2012-13Dr. Lakshmi Sridhar
18th Sep 2012
Sodium hyaluronate –Is a linear chain muco-polysaccharide composed of disaccharide units formed from D-glucuronic acid & N acetylglucosamine held together by 1,3 linkages
• Fermentation: Streptococcus equi, subsp.
•Bacterial strain, non-haemolytic
• Non-GMO
•No animal products used during the manufacturing process
Source
BIO/001/2012-13Dr. Lakshmi Sridhar
18th Sep 2012
Process Flow Diagram
BIO/001/2012-13Dr. Lakshmi Sridhar
18th Sep 2012
Sodium Hyaluronate - specification
S.No. Tests Specification
1. Appearance White powder or granules
2.Transparency of 0.1% aqueous
solution
NLT 99.5%
3. Loss on drying NMT 20%
4. Residue on Ignition NMT 20%
5. pH (0.1% aqueous solution) 5.0 – 8.0
6. Protein content NMT 0.1%
7. Glucuronic acid 44 – 50 %
8. Sodium Hyaluronate 93 – 103 %
9. Molecular weight 1.0 – 1.6 MDa
10. Total bacterial count NMT 100 cfu/g
BIO/001/2012-13Dr. Lakshmi Sridhar
18th Sep 2012
Sodium hyaluronate in the body
BIO/002/2012-13Dr. Lakshmi Sridhar
18th Sep 2012
• Hyaluronic acid – significant constituent of human skin, connective tissue, lubricant in joints, vitreous humor of the eye
Sodium Hyaluronate
BIO/002/2012-13Dr. Lakshmi Sridhar
18th Sep 2012
• Skin has 10 to 20 % water• As we age, water is lost & the skin appears dry & wrinkled• Great ability to hold water – 1000 times its weight• Forms a film on the surface of skin to moisturize • Smoothens the skin – anti-wrinkle effects
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15 30 60 120 180 240 300 360
Hyd
rati
on
Incr
ease
%
Time (mins)
0.025% Kopuron Max: Skin Moisturizing Effect
Sodium Hyaluronate
BIO/002/2012-13Dr. Lakshmi Sridhar
18th Sep 2012
Comparison between Sodium hyaluronate & other moisturizers under identical conditions
0 5 10 15 20 25 30 35 40
Hydration Increase %
Sodium pyrrolidonecyboxylate
Glycerine
Sorbitol
Kopuron Max
Sodium Hyaluronate
BIO/002/2012-13Dr. Lakshmi Sridhar
18th Sep 2012
• Moisturizing property used in cosmetics for sun care, skin care, repair, lipsticks, pre & after shave, cleansers, hair care, etc.
Sodium hyaluronate
BIO/002/2012-13Dr. Lakshmi Sridhar
18th Sep 2012
Transparency – of 1 % aqueous solution of Sodium hyaluronate was measured at 660 nm – transparency of 99.99 % was measured – very transparent – ideal for cosmetic applications
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400 nm
600 nm
Ab
sorb
ance
%
Sodium Hyaluronate
Heat Stability – Aqueous solution of Sodium hyaluronate was incubated at different temperatures for different times. Corresponding changes in kinematic viscosity were measured. Sodium hyaluronate is stable at temperatures lower than 80 C
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0 15 30 45 60 75 90
Kin
em
ati
c V
isc
osi
ty
Time (mins)
Kopuron Max: Heat Stability
60 deg C
80 deg C
90 deg C
BIO/002/2012-13Dr. Lakshmi Sridhar
18th Sep 2012
Sodium Hyaluronate
BIO/002/2012-13Dr. Lakshmi Sridhar
18th Sep 2012
• Sodium hyaluronate’s moisturizing property demonstrated in lip sticks using corneum hydration measurements. Hydration increases with Kopuron Max
Sodium Hyaluronate
BIO/002/2012-13Dr. Lakshmi Sridhar
18th Sep 2012
• Sodium hyaluronate in lipsticks decreases trans epidermal water loss (TEWL). Kopuron reduces TEWL.
SODIUM HYALURONATE
BIO/002/2012-13Dr. Lakshmi Sridhar
18th Sep 2012
Biodegradable & eco-friendly
Sodium hyaluronate is completely natural
Produced by bacterial fermentation
“Green technology”