coshh assessment form - university of sheffieldmedicine.dept.shef.ac.uk/coshh2/pdfs/243.pdf ·...

School of Medicine Biomedical Sciences

COSHH Assessment Form

Tracking No: 1 Labs Involved: All

Title of Procedure: Preparation and general use of Disinfectant containers for bench use

Brief Description:

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalSodium hydroxide Corrosive, causes severe burns. 50ml of 5M solution Medium Wear lab coat,

gloves and eye protection.

Sink with copious amounts of water

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions Disposal70% IMS (meths) flammable <1litre Low Wear buttoned up

lab coat and gloves. Keep away fr9m naked flames

Drain with copious amounts of water

Trigene II NON 1L Conc use at 2-8% solution 500ml spray at 2%

Low Wear buttoned up lab coat and nitrile gloves


Ringers soln none 500ml Low wear lab coat down sink with copious amounts of water

AquaClean Non-hazardous 250ml Low wear buttoned up lab coat and nitrile gloves


Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalMalonyl coenzyme A lithium salt

This substance is not classified as dangerous according to Directive 67/548/EEC.

1g Low wear buttoned up lab coat

down sink with copious amounts of water

(±)-α-Tocopherol This substance is not classified as dangerous according to Directive 67/548/EEC.



G4796 Toxic if swallowed.Causes serious eye irritation.

1g High wear buttoned up lab coat, gloves and eye protection

hazardous waste route

LRRK2-IN-1 Not hazardous according to European Union Directive 67/548/EC and 1999/45/EC



Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalBiocidal ZF irritant 1L spray bottle Low wear buttoned up

lab coat and nitrile gloves


Virkon (and other peroxide containing disinfectants)

Irritant when in powder form. 1L 1% solution Low Wear lab coat, gloves and also face mask when removing powder from stock container


Virusolve (concetrate diluted to 10% working conc)

Irritant to eyes and respiratory system, bleaches clothing 5L concentrate Low Wear gloves, Weat buttoned up lab coat and nitrile gloves

Sink - with copious amounts of water.

L-2-Oxothiazolidine-4-carboxylic acid

May cause dermatitis, skin and respiratory tract irritation. May be harmful if swallowed. Avoid contact with eyes

5g Low wear buttoned up lab coat and nitrile gloves and eye protection


Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalPrecept tablets Irritant to eyes skin and respiratory system. Contact with

acides liberates toxic gas250g (each tablet = 2.5g)

low wear buttoned up lab coat and nitrile gloves. Do not store as a solution in a special container as chlorine gas may build up

sink with copious amounts of water

Microsol 3 plus none 5L Low Wear buttoned up lab coat and nitrile gloves

Biologically degradable with 6 month shelf life. Down sink with copious amounts of water

Bleach irritant to eyes, skin and respiratory system. Contact with acids liberates toxic gas

10L Low wear buttoned up lab coat and nitrile gloves


Good Laboratory practice

Special Precautions:

The disposal of all waste should be undertaken in accordance with local regulations

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions Disposal

Do not use Virkon in a metal containeras it is corrosive. Do not store presept solution in a sealed container as chlorine gas builds up.

Assessed Sue Smith Date 08/10/2012Approved by: Date



Tracking No: 2 Labs Involved: DU30

Title of Procedure: Flow cytometry

Brief Description:

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalPBS tablets none 50tablets Low Good laboratory

practice; gloves, lab coat etc

Flush down drain with large quantities of water

Mouse Human Rat Ig antibodies

"possible contact sensitivity, avoid skin contact" 10ul low Good laboratory practice; gloves, lab coat etc


"Antibodies labelled with FITC, phycoerythrin (PE), APC, Cy3, PECy7, APCCy7, Pacific Blue, AmCyqa, Cy5, PE-Cy5, PECy5.5"

"possible contact sensitivity, avoid skin contact" 20ul low Good laboratory practice; gloves, lab coat etc


"Propidium iodide, BrDU, TOPRO3, Hoescht"

"potential carcinogens, avoid skin contact" low Good laboratory practice; gloves, lab coat etc


Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalAlexa - fluorescently labelled secondary antibody

"possible contact sensitivity, avoid skin contact" low Good laboratory practice; gloves, lab coat etc


Ethanol Flammable low Good laboratory practice; gloves, lab coat etc


Fix & permeabilisation kit Harmful if ingested low Good laboratory practice; gloves, lab coat etc


Goat mouse serum Biohazard low Good laboratory practice; gloves, lab coat etc


Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalSaponin Very toxic by inhalation irritating to eyes Medium Good laboratory



Syto 13 "Potential carcinogen, avoid contact with skin" low Good laboratory practice; gloves, lab coat etc


Triton X "possible contact sensitivity, avoid skin contact" low Good laboratory practice; gloves, lab coat etc

YO-PRO-1 iodide in 1mM DMSO

Skin Absorption <1ml Low Good laboratorypractice; gloves, labcoat etc Use in well ventilated area

Orange waste bin

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalMitoTracker Deep Red FM

No known hazard according to Invitrogen MSDS <1ml Low Good laboratorypractice; gloves, labcoat etc

Orange bag / sink

Paraformaldehyde Flammable, carcinogen, toxic to skin & respiratory sysyem <10g Medium Good laboratory practice; gloves, lab coat etc


5-(and-6)-carboxyfluorescein diacetate, succinimidyl ester

good laboratory practice <1ml low Good laboratory practice; gloves, lab coat etc

2'',7'-bis-(carboxyethyl0-5(6')-carboxyfluoroscein acetoxymethyl ester (BCECF-AM)

respiratory tract and eye irritant 1mg Medium Good laboratory practice; gloves, lab coat etc


Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalSodium azide Harmful by inhalation, care when weighing (use fume hood) 20ml 0.01% Medium Good laboratory



FACSClean Medium Good laboratory practice; gloves, lab coat etc


Precept tablets Medium Good laboratory practice; gloves, lab coat etc


FACSRinse "possible contact sensitivity, avoid skin contact" low Good laboratory practice; gloves, lab coat etc


Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalFACSFlow "possible contact sensitivity, avoid skin contact" low Good laboratory



CBA flex sets and buffer kits

"possible contact sensitivity, avoid skin contact" Low Good laboratory practice; gloves, lab coat etc


Animal & human cell lines, misc.primary cell lines, Human tissue and serum

"Biohazard- category 2 containment, treat as a potential hazard of infection"

Medium Wear a buttoned up lab coat and gloves. Employ aseptic technique within the confines of a ClassII cabinet.

Orange box/ bin after bleaching for 30mins (fluids down sink after bleaching). Tissues should be in sealed containers , re-lid T/C flasks.

Detachabeads CD19 None - Low Good laboratory practice.

Orange bin or dilute solutions down sink/sluice with copious amounts of water.

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalErythrolysis Red Cell Lysing Buffer

TOXIC - Low Good laboratory practice.


Red Cell Lysing Reagent None - Low Good laboratory practice.


Golgi Plug toxic to skin & respiratory sysyem <10g Low Good laboratory practice; gloves, lab coat etc

Orange bin

PKH26 Red Fluorescent cell dye

Flammable <1ml Low Wear lab coat & gloves

Orange bag / sink



Avoid contact with acids of solutions containing Sodium azide.

Assessed A.Heath Date 01/05/2008


Approved by: Date

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalBafilomycin A1 Irritating to eyes, respiratory system and skin <1ml Low Good laboratory

practice; gloves, labcoat etc

Flush down drainwith large quantitiesof water



Tracking No: 3 Labs Involved: All staff

Title of Procedure: pH adjustment of culture media,solutions, buffers & reagents

Brief Description:Chemicals added drop by drop, either as concentrates or molar solutions.

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalAcetic acid (Glacial) Flammable. Causes severe burns. <250ml Medium Decant from stock in

fume cupboard. Wear lab coat, gloves and eye protection.


Hydrochloric acid "Corrosive, toxic, causes severe burns, extremly irritating to eyes & respiratory system"

1-10ml Medium Decant from stock in fume cupboard. Wear lab coat, gloves and eye protection.


Potassium Hydroxide Harmful if swallowed. Causes severe burns 50ml of 5M solution Medium Wear lab coat, gloves and eye protection.




The above chemicals must not come into contact with metal or metal alloys as hydrogen may form which can be explosive.

Assessed John Anson Date 11/12/2012


Approved by: Date

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalSNARF4 Carboxy acid pH indicator

None 20 x 50ug Low Wear lab coat & gloves


Nigericin Toxic. Irritant 10mg Low Wear gloves & lab coat

Sink with copious amounts of water.




Title of Procedure: "Surface and cabinet sterilisation / fumigation in tissue culture and category 2, 3 rooms"

Brief Description:The fumigation of class I and II safety cabinets/ class III facility for decontamination purposes

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalFormaldehyde or formalin

harmful if ingested, inhaled or absorbed through skin. Inhalation may be fatal. May cause allergic respiratory and skin reactions long term WEL=2ppm, short term WEL=2ppm

2.5L High wear unpowdered nitrile gloves and do not handle outside class 1 cabinet or fume hood. Ensure cabinet and fume hood working before use. Only trained personel to carry out this procedure

Formaldehyde evaporates during procedure

virusolve + concentrate irritant to eyes and skin 1 litre,10% soln Medium wear powder free nitrile glovesand suitable face protection,do not mix with other chemicals

rinse container prior to disposal



Medical attention should be sort in the event of exposure to fumes

Assessed Katie Cooke Date 12/04/2008


Approved by: Date





Title of Procedure: Tissue culture of animal and mammalian cell lines including human stem cells.

Brief Description:Isolation, culture, long-term maintenance and storage of cells from human and animal origin.

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions Disposal"Antibiotics (in solution) e.g. penicillin, streptomycin, gentamicin, vancomycin, fungizone ,Zeocin, geneticin, ciprofloxacin, chloroquinine

Low risk /Harmful if swallowed or in contact with skin. May cause sensitization.

Stock 20ml - 100ml Use 30ul to 5ml

Low

"Cell culture media e.g. ALPHA,RPMI, DMEM, EMEM, Ham's F12, MEM, M199, EBSS, X-VIVO 20, Hanks,Glasgow,D-Valine.Knockout DMEM, SR, Airway Epithelial Cell Basal Medium"Neurobasal™-A Medium (1X) Liquid without Phenol Red M2

Not Hazardous according to directive 67/548/EEC 500ml Low

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalAnimal & human cell lines, misc.primary cell lines, Human tissue and serum

"Biohazard- category 2 containment, treat as a potential hazard of infection"

Low .

Sera ; FCS, New born, sheep, goat, de-fibrinated horse blood

Biohazard max 50ml/500ml media

Low

L-Glutamine Not Hazardous according to directive 67/548/EEC 5ml/500ml media Low

b-mercaptoethanol Harmful by inhalation, ingestion or skin adsorption. Can cause sensitisation.

<1ul Low When pipetting from stock ensure that tips etc. are sealed in bags and disposed of in orange clinical waste box.

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalPotassium chloride None 3g Low

Non essential amino acids (NEAA)

None / low risk 5ml/500ml media Low

Sodium azide Very toxic by ingestion, inhalation, irritating to skin & eyes 0.05% Low Do not breath dust

Trypan blue Suspected teratogen, may cause cancer 4ul/sample Low

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalTrypsin / EDTA Versene) Irritating to eyes/skin/respiritory system -may cause

sensitization by repeated exposure.5ml Low

Forskolin Irritating to skin & eyes, harmful by ingestion, penetrates skin 10uM Low Place in container and dispose of in orange bag.

DMSO Irritating to skin & eyes, harnmful by ingestion, penetrates skin 10%:100ul/1ml media

Low

Cell dissociation solution No hazards listed 500ml stock. Low

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalSodium Buyrate None identified Low

Rapamycin, from Streptomyces hygroscopicus


1mg Low

BM Cyclin Contains no hazardous substances in concentrations to be declared

1g Low

H


Zoledronic acid High irritant to skin/eyes and inhalation. Chronic health effects if prolonged exposure.

1g Low Wear buttoned up labcoat, gloves and eyeprotection

DO NOT let wash water enter drains. Burial in landfill. Consult manufacturer for recycle options

Rat OPG.Fc None identified 4.56 mg/ml Low Wear buttoned up labcoat, gloves and eyeprotection

Sink with plenty of water

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalVybrant CM-DiI cell labelling solution (contains ethanol)

Flammable 1ml Low Wear buttoned up labcoat, gloves and eyeprotection.Avoid breathing vapours. Keep away from sources of ignition.


Vybrant DID cell labelling solution (contains ethanol)

Flammable. 1ml Low Wear buttoned up labcoat, gloves and eyeprotection.Avoid breathing vapours. Keep away from sources of ignition.


Murine soluble RANKL May cause irritation to skin and eyes. 500ug Low Wear buttoned up labcoat, gloves and eyeprotection.

Observe local environmental regulations. Contact a licensed professional waste disposalservice to dispose of this material.

Chloroquine Harmful if swallowed. 1g Low

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalAquaclean microbiocidal additive for water bath

Weakly irritant for skin. Irritant for eyes and mucosa 250ml Low Wear eye protection when handling stock solution.

Picropodophyllotoxin Toxic, harmful in contact with skin, irritating to eyes respiratory system and skin, possible irreversible effects

1g High Wear eye protection. Via safety services

D-(+)-Galactose This substance is not classified as dangerous according to Directive 67/548/EEC.

100G Low

MAP Kinase inhibitor (SB 203580)

Harmful if swallowed. Risk of serious damage to eyes 1mg Low Orange clinical waste box

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalcAMP dependent protein kinase inhibitor

Non hazardous peptide from synthetic source 0.5mg Low Orange clinical waste bin

MycoAlert reagent May cause irritation to skin,eyes and respiratory tract 3ml Low Small quantities of all reagents mixed. Final vol <2mls. Soak plate and containers in virkon for 2hr then dispose in offensive waste

MycoAlert Assay buffer May cause mild irritation to skin,eyes and respiratory tract 10ml Low Small quantities of all reagents mixed. Final vol <2mls. Soak plate and containers in virkon for 2hr then dispose in orange clinical waste

MycoAlert substrate May cause irritation to skin,eyes and respiratory tract 3ml Low Small quantities of all reagents mixed. Final vol <2mls. Soak plate and containers in virkon for 2hr then dispose in offensive waste

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalCell culture supernatant Potential biohazard 2ml Low Wear unpowdered

nitrile glovesSmall quantities of all reagents mixed. Final vol <2mls. Soak plate and containers in virkon for 2hr then dispose in orange clinical waste

Uridine Not a hazardous substance or mixture according to EC-directives 67/548/EEC or 1999/45/EC.


Poly-DL-ornithine hydrobromide


25mg Low

Anti-GLAST (ACSA-1)-Biotin, mouse, rat

This mixture is not classified as dangerous according to Directive 1999/45/EC.

2ml Low

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalAG 1024 Hazards not identified must be considered potentially

hazardous1g Medium Wear eye

protection. Handle in fume hood.

Via safety services

CytoTox-ONE™ Homogeneous Membrane Int. Assay

not hazardousaccording to Directive 67/548/EEC or Directive 1999/45/EC

1kit Low

Aicar Toxicological/hazardous properties not fully investigated, treat as toxic

1g High Wear eye protection hazardous waste route (what is this)

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalSodium palmitate Irritating to eyes 1g Medium via safety services

L-Carnitine inner salt Irritating to eyes, respiratory system and skin. 1g Medium

Estrogen Receptor antagonists: MPP Dihydrochloride, PHTPP, G-15

Exposure may cause irritation to eyes, mucous membranes, upper respiratory tract and skin. Toxicology not fully investigated.

< 20mg Low Use in fume cupboard where possible.

Orange clinical waste bin.

Atorvastatin This substance is not classified as dangerous according to Directive 67/548/EEC.

5g Low


Folinic acid calcium salt hydrate

Harmful. May cause sensitisation by repeated contact. Eye, skin and respiratory irritant.

250mg Low Avoid strong oxidising agents. Hygroscopic and light sensitive.

Bafilommycin A1 Irritating to eyes, respiratory system and skin 2µg Low Wear eye protection

Natural mouse laminin The product contains no substances which at their given concentration, are considered to be hazardous to health

1g Low

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions Disposal6-Carboxy-2',7'-Dichlorodihydrofluorescein Diacetate, Di(Acetoxymethyl Ester) (DCF)

The product contains no substances which at their given concentration, are considered to be hazardous to health

5mg Low Use personal protective equipment

Solids - via offensive waste route. Dilute solutions down sink with copious amounts of water.

EthD1: ethidium homodimer-1

The product contains no substances which at their given concentration, are considered to be hazardous to health.

1mg Medium Good laboratory practise. Wear gloves, buttoned up lab coat and eye protection when handling.

Via Hazardous waste route. Prevent product from entering drains.

Optiprep None 40ml Low

Lymphoprep [Aprotonin] Not hazardous according to Directive 67/548/eec 50ml Low

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalInsulin-Transferrin-Selinium G suppliment x100

No hazardous componenets 100ml Low down sink with copious amounts of water

N2 Supplement none 10ml Low none down sink with copious amounts of water

Purmorphamine Tox properties not fully evaluated. Treat as potentially hazardous, irritating

1g Medium wear gloves, goggles treat as toxic

as per waste assessment

LDN193189 Moderate to severe irritant to the skin and eyes. 1g Medium wear gloves and eye protection


Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalCollagenase Irritating to eyes skin and respiratory system 5ml Low

Iodoacetamide Toxic if swallowed. May cause sensitisation by inhalation and skin contact.

5mM in 10-20ml Low Avoid breathing dust. Avoid skin/eye splashes.

1alpha25 Dihydroxyvitamin D3

Toxic by inhalation, contact with skin and ingestion 10uM Low

MIP-1 alpha (CCL3) None <10ug/ml in PBS Low

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalMIP-1 beta (CCL5) None >10ug/ml in PBS Low

PBS Not hazardous according to directive 67/548/EEC 500ml Low

Mitomycin -C Highly toxic. May cause cancer and heritable genetic damage. Very toxic by inhalation, in contact with skin and if swallowed. Irritating to eyes, respiratory system and skin.

10ug/ml Low Wear suitable eye/face protection.

Place in container and dispose of in orange bag.

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalGrowth factors Bfgf, FGF4, BMP4, , GDNF.Additionally Oncostatin M, LIF, recombinant RANK ligand, CNTF, BDNF

The chemical, physical and toxicological properties have not been thoroughly investigated.

10ng/ml Low Place in container and dispose of in orange bag.

Stem Cell Factor (SCF, c-Kit Ligand) FSH, LH.

The chemical, physical and toxicological properties have not been thoroughly investigated.

10ng/ml Low Place in container and dispose of in orange bag.

Retinoic acid Harmful if swallowed 20ug Low Place in container and dispose of in orange bag.

Granulcyte- Macrophage Colony Stimulating Factor (GM-CSF)

Small quantities used 1ug/ml Low

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalIL-4 Small quantites used 1ug/ml Low

Ethanol Flammable 250mls Low Avoid breathing vapours. Keep away from sources of ignition.

AICA-Riboside No specific hazard 100ml Low

Akt Inhibitors VIII and IV Eye/Skin Irritant. Causes blistering. 1ml Medium

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalPhenformin HCl Harmful if swallowed 10ml Low

Calcium Ionophore Irritant to eyes/skin. 10ml Low

Ionomycin Not hazardous according to Directive 67/548/EEC 1ml Low

Promega Cell Titre 96 Aqueous One Solution Assay

Not fully investigated, currently not hazardous according to directive 67/548/EEC

I Kit Low

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalLiquid nitrogen refer to COSHH 7

IL-1 Small quantites used 1ug/ml Low

IL-2 Small quantites used 1ug/ml Low

dispase Irritating to eyes skin and respiratory system 5ml Low sweep up and dispose of in orange bin

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalPokeweed mitogen skin irritant,carcinogen Low sweep up and dispose of

in orange bin

HEPES None - Low

Epidermal Growth Factor None - Low Orange bin or dilute solutions down sink/sluice with copious amounts of water.

Gelatin None - Low Orange bin or dilute solutions down sink/sluice with copious amounts of water.

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalGly-his-lys-acetate None - Low Orange bin or dilute

solutions down sink/sluice with copious amounts of water.

Hydrocortisone None - Low Orange bin or dilute solutions down sink/sluice with copious amounts of water.

Insulin None - Low Orange bin or dilute solutions down sink/sluice with copious amounts of water.

Interferon None - Low Orange bin or dilute solutions down sink/sluice with copious amounts of water.

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalPHA None - Low Orange bin or dilute


Platelet Derived Growth Factor

None - Low Orange bin or dilute solutions down sink/sluice with copious amounts of water.

Thrombin None - Low Orange bin or dilute solutions down sink/sluice with copious amounts of water.

Thyrotrophin (TSH) None - Low Orange bin or dilute solutions down sink/sluice with copious amounts of water.

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalTransferrin None - Low Orange bin or dilute


Tumour necrosis factor None - Low Orange bin or dilute solutions down sink/sluice with copious amounts of water.

Sodium Hydrogen carbonate

None Low

Calcium chloride None 100ml Low

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalPotassium dihydrogen phosphate

irritant 1g Low

Disodium Hydrogen Carbonate

None 10g Low

Collagen None - Low Orange bin or dilute solutions down sink/sluice with copious amounts of water.

Transferrin None - Low Orange bin or dilute solutions down sink/sluice with copious amounts of water.

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalBacitracin None - Low Orange bin or dilute


Fibronectin None - Low Orange bin or dilute solutions down sink/sluice with copious amounts of water.

Sodium Pyruvate None - Low Orange bin or dilute solutions down sink/sluice with copious amounts of water.

Poly L Lysine None - Low Orange bin or dilute solutions down sink/sluice with copious amounts of water.

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalBSA None - Low Orange bin or dilute


Microsol None - Low Orange bin or dilute solutions down sink/sluice with copious amounts of water.

Dexamethasone Irritant to eyes/skin, inhalation <1g Low Wear eye protection. Do not breath powder

Octreotide None - Low Orange bin or dilute solutions down sink/sluice with copious amounts of water.

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalBovine Pituitary Extract May possibly be an irritant trace Low

Epidermal Growth Factor Irritant trace Low

Hydrocortisone Irritant trace Low

Insulin Irritant Trace Low

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalThyroxine Irritant Trace Low

Myclone Low-risk 1ml Low Orange bin

Lectin None - Low

Pepsin Irritant - Low Orange bin or dilute solutions down sink/sluice with copious amounts of water.

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalLipopolysaccharide Harmful - Low Orange bin or dilute


17B-Estradiol Estragen Carcinogen, Mutagen Teratogen. Targets reproductive system. May cause congenital malformations

Low Wear goggles. Do not breath powder

Orange bin

Toll like receptors Unknown Low

Sodium selenite Toxic <1g Low

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalEndothelial cell growth supplement

None identified 50mg Low

Heparin sodium salt None <1g Low

Gelatin Harmful 0.2% solution / 100g stock

Low

Farnsyl pyrophosphate Toxic. Harmful <1ml Low

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalElastin Harmful <1ml Low

EDTA Harmful 250g Low

Antibody coated beads May contain sodium azide <1ml Low Orange bag

5-bromo-2'deoxyuridine Toxic. Potential mutagen & teratogen <1ml Low

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalCaspase inhibitors None identified <1ml Low

Geranylgeranyl pyrophosphate

Toxic 1ml Low

D-mannitol Irritant 100g Low

Elastase Harmful <1g Low

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalCalcein AM None <1ml Low

Angiogenesis kit Potentially pathogenic kit Low

Gold chloride Corrosive 1ml solution Low

Monensin sodium salt None <1g Low

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalC. botulinium C3 exoenzyme

Toxic <1g Low Orange bag

C. tetani tetanolysin Toxic <1g Low Orange bag

Lactacystin None <1g Low

Sodium Oxalate Harmful & Irritant <100g Low

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalAngiotensin II None <1ml Low

Biotin Irritant <1ml Low

MesNa Irritant <1ml Low

Cycloheximide Toxic <1g Low

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalStaurosporine, streptomyces sp. In solution

WARNING! Harmful if swallowed, ingestion may be fatal. May cause eye skin irritation - itching, scaling,reddening or occasionally blistering. Repeated exposure may result in an accumulation in one or many human organs.

1ml in DMSO Medium Store locked up away from sources of heat / ignition. Wear eye protection.

Microlitre amounts used in assay will be disposed of in sealed assay plates in Orange bags for incineration.

MitoProbe JC-1 Assay kit Harmful if inhaled or swallowed. Irritating to eyes and skin. 125ul Low Store desiccated at -20C. Also see CoSHH 2.

Micro litre amounts will be disposed of in tubes/ wells into orange waste bags.

Butyric Acid Corrosive - causes burns 5ml Low Do not breathe vapour.

Valeric acid CorrosiveMay be harmful if inhaled, ingested or absorbed through skin

1ml Low Do not breathe vapour.Wear gloves, goggles and lab coat.Use in class II cabinet.

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalPropionic Acid Corrosive -Causes burns 1L Low Do not breathe

vapour.Wear eye protection.

Trypsin / Chymotrypsin Irritant 20ug Low

Y-27632 dihydrochloride Harmful 10mg Medium wear gloves and eye protection


Mineral Oil Not a hazardous substance or mixture according to Regulation (EC) No. 1272/2008.This substance is not classified as dangerous according to Directive 67/548/EEC.

100ml Low Good laboratory practice

Non-hazardous waste route

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions Disposal5%Carbon Dioxide / 5% Oxygen / Nitrogen 200BAR

Compressed gas, asphyxiant 1 cylinder High Wear eye protection , use with O2 meter. All users must be trained and follow School SOP and risk assessment

Used up in course of experiment. Cylinder returned to BOC

% Oxygen/Nitrogen 200bar

Compressed gas, asphyxiant 1 cylinder High wear eye protection, use with O2 meter, All users must be trained and use with School SOP and risk assessment

Gas used up during experiment and cylinder returned to BOC

Torin 1 The product does not need to be labelled in accordance with EC directives or respective national laws.

1g Low none down sink with copious amts of water

EmbryoMax® KSOM Embryo Culture (1X), Powder, w/o Phenol Red

Toxic To Reproduction .Serious Eye Damage/Irritation .Skin Corrosion/Irritation .Acute Toxicity – Oral

50ml High wear gloves. Women of reproductive age must be considered to be pregnant and need to handle substance accordingly

Hazardous waste route/safety services

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalValproic Acid 92-propylpentanoic acid sodium)

Irritating to eyes, skin and respiratory system. Harmful if swallowed.

10g Low Handle within the confines of a fume hood.

Micro litre amounts contained in tubes/wells in orange waste bags.

Alpha compound 8 Irritating to eyes, skin and respiratory system. Harmful if swallowed.

10g Low Micro litre amounts contained in tubes/wells in Orange Bag.

CHAHA (Sigma product C2368)

Irritating to eyes, skin and respiratory system. Harmful if swallowed.

1mg Low Micro litre amounts contained in tubes/wells in orange bags.

Sirtinol Irritating to eyes, skin and respiratory system. 1mg Low Avoid prolonged or repeated exposure.

Micro litre amounts in tubes/wells in orange bags.

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalScriptaid Not hazardous according to Directive 67/548/EEC 1mg Low Microlite amounts will be

disposed of in tissue culture medium, either down the sink after bleaching, or contained in T/C plasics in orange bags

Methotrexate Possible harm to unborn child, toxic if swallowed, irritating to eyes and skin.

5 umole Low Handle within the confines of a fume hood.

Klean-Prep(contains PEG,Anhydrous sodium sulphate,sodium bicarbonate,sodium chloride,potassium chloride)

Causes laxative effect if consumed in quantity, therefore avoid consumption.

100ls of 10% solution.

Low

Picolax (contains sodium picosulfate, magnesium oxide, citric acid)

Product has a laxative effect, therefore avoid ingestion. Avoid skin/eye splashes.

100mls of a 10% solution

Low

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalHistopaque 1077 May cause sensitisation by inhalation and skin contact 500ml Low Place in container and

dispose of in orange bag

Aspirin Irritating to eyes, respiratory system and skin. Harmful if swallowed

2mM Low

Ribonuclease A Irritating to eyes, respiratory system and skin. May cause sensitization in sensitive individuals.

10mg Medium Avoid skin.eye contact. Do not breathe fumes. Wear goggles.

Microlitre amounts will be disposed of in T/C vessles placed in orange bags. Absorb spills on vermiculite and place in fumehood to evapourate.

2-Deoxy-D-Glucose None 1g Low

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalOligomycin Harmful. Toxic 5mg Low Use in a fume hood.

Avoid breathing dust.

MG-132 None 5mg Low Orange bag / sink with copious amounts of water

Lumiflavine Not dangerous according to directive 67/548/EEC 25mg Low Do not breathe dust. Avoid skin/eye splahes.

Nutridoma- SP None 100ml stock, 1ml per experiment

Low

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalAlamar Blue None 25ml Low

SigmaClean Corrosive Flammable 4fluid ounce Low Store and use away from sources of ugnition.

L-Ascorbic acid none 100mg/10ml Low

Isoton II Diluent None 5L Low

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions Disposal2'7'-Dichlorofluorescin diacetate

Not hazardous according to Directive67/548/EEC. Not thououghly investigated.

50mg Low Avoid dust formation. Air and light sensitive. Store away from strong oxidizing agents.

Very small amounts in tissue culture media in orange bag/box. Do not let neat product enter drains.

1-Thioglycerol Harmful if swallowed. Toxic in contact with skin. Irritant to eyes, skin and respiratory system.

25ml Low Prepare in fume hood.

Bathocuproinedisulfonic acid disodium

Irritant to eyes/skin and respiratory system. 500mg Low

Bisindolylmaleimide I May be an irritant, may cause irritation to eyes, nose and mucous membranes

10mM stock Low

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalNeurobasal Not hazardous (1999/45/EC) 500ml Low

B27 Not hazardous (1999/45/EC) 10ml Low

Bisindolylmaleimide IMay be an irritant, may cause irritation to eyes, nose and mucous membranes

10µl 10mM Low

Dow corning high vacuum grease

May be irritant to eyes 50g Low in orange bag. DO NOT put down sink.

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalCustom made synthetic peptides

chemical, physical and toxicological properties have not been fully investigated.

10ml High Do not release spilled or wasted material to the environment. This material should be handled in a restricted manner until its degree of risk has been adequately assessed. Exercise due care Do not

orange bags

GEY'S BALANCED SALT SOLUTION

Irritant to skin, eyes, mucus memberanes and respiratory tract. Harmful by ingestion and absorption through skin

500ml Low

95% O2/ 5% CO2 Compressed gas, supports combustion, potential explosion hazard

1 cylinder Medium Refer to Risk assesment 20 and SOP 20

Return cylinder to supplier when finished.

Lactacystin not classified as dangerous according to directive 67/548/EEC 5g Low

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalProteasome substrates II, III and IV

No specific hazard 5g Low

cyquant proliferation kit-hazardous components, dye deliery reagent and dye reagent

Harmful if swallowed, possible irreversible effects, irritant to eye and skin

1 kit Medium Wear eye protection Assay plates disposed of in orange bins as contain biological samples

Glucose solution Not hazardous according to Directive 67/548/EEC. 100ml Low

Holo Transferrin (Bovine) Not a hazardous substance or preparation according to EC-directives 67/548/EEC or 1999/45/EC.

50mg Low

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalMycoplasma removal agent

Not classified as hazardous under UK regulations and 29 CFR1910.1200 ( OSHA regulations)

500ml Low

Compressed CO2 vapour withdrawel

Compressed gas, asphyxiant 6 cylinders Medium Refer to risk assesment 20 and SOP 08.

cylinders returned to BOC after use

Deoxyribonuclease I Not classified as dangerous accordig to directive 67/548/EEC 15KU Low

Trypsin inhibitor ot classified as dagerous according to directive 67/548/EEC 25mg Low

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions Disposal5-aza-2'- deoxycytidine Harmful, Irritant 5 mg Medium

Chelerythrine chloride Irritant to skin, eyes, and respiratory tract. Harmful if ingested 200mg Low Wear eye protection.

nuclear extract none 1ml Low

whole cell lysate none 1ml Low

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalBromopyruvic acid Corrosive, causes burns 1g High Wear eye

protection. Handle in fume hood or TC hood with sash down. Check fume hood on and working before.

Hazardous chemical waste route (what is this ).

Sodium oxamate This substance is not classified as dangerous according to Directive 67/548/EEC.

1g Low

Sodium iodoacetate Toxic if swallowed. Risk of serious damage to eyes. Irritating to respiratory system and skin.

1g Medium wear buttoned up lab coat, gloves and eye protection. Handle in fume hood or TC hood with sash down. Check fume hood on and working before.


Sodium fluoride Toxic if swallowed. Irritating to eyes and skin. Contact with acids liberates very toxic gas.

1g Medium Handle in fume hood or T/C hood with sash down. Check fume hood on and working. AVOID CONTACT WITH ACIDS.

Hazardous chemical waste route



It is assumed that good laboratory practice will be adhered to. The majority of the reagents listed in this procedure will be added to the culture media. Therefore in most instances the waste will be treated with disinfectant prior to disposal. Liquid waste will be disposed of down the sink/sluice with copious amounts of water unless otherwise specified. Where larger volumes/masses are involved, an alternative disposal route may

Assessed Vanessa Singleton Date 09/10/2012


Approved by: Date




Title of Procedure: Stimulation/Transfection of animal and mammalian cell line-see also COSHH no. 5

Brief Description:Transfection is carried out by using various agents to allow the introduction of cloned DNA into cultured mammalian cells. Stimulation is carried out independantly.

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalCalcium chloride None / low risk 2.5ml Low Wear lab coat and

chemical; resistant gloves


Hepes None / low risk <3g Low Wear lab coat and nitrile gloves

Liquid form down sink with plenty of water. Solid form - orange biological waste bag

DEAE dextran "Harmful by inhalation, or skin adsorption if used in large quantities. Possible teratogen"

50g Low Wear lab coat and nitrile gloves

Orange biological waste bag

Lipofectin / Lipofectamine

None 2-4ul/sample Low Wear lab coat and nitrile gloves


Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalSuperfect None / low risk 1.2ml Low Wear lab coat and

nitrile glovesOrange biological waste bag

DMRIE-C low risk <100ul Low Wear lab coat and nitrile gloves


Disodium hydrogen phosphate

None / low risk <1g Wear lab coat and nitrile gloves

Orange biological waste bag. Liquid waste pour down drains with copious amounts of water.

Dihydrotestosterone,Testosterone, and analogues

Harmful by skin contact and inhalation. Skin irritant.Carcinogen, teratogen, may cause harm to unborn child

1mg Low Wear lab coat and nitrile gloves

purple bin

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalEDTA None / low risk 2g Low Wear lab coat and

nitrile glovesLiquid Waste - down sink with plenty of water. Solid waste Orange biological waste bag

Endothelial cell growth factor

None 10ul Low Wear lab coat and nitrile gloves

Orange Clinical waste bag

ethanolamine Skin & eye irritant. Wear lab coat and nitrile gloves

Gelatin None 100ul Low Wear lab coat and nitrile gloves

Orange bin

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalHAT supplement * "May be fatal if inhaled, ingested or adsorbed through skin" 100ml Wear lab coat and

nitrile gloves

Hygromycin B * Very toxic by inhalation or on contact with skin. May cause sensitisation

1g stock Low Wear lab coat and nitrile gloves


Immunoglobulins from various sources

Biohazard 1ml Low Wear lab coat and nitrile gloves

YELLOW BIN

Micro-carrier beads None Wear lab coat and nitrile gloves

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalPhenylarsine oxide "Causes burns; very toxic by inhalation, contact with skin or if

ingested. Possible irriversible effects"Wear lab coat and nitrile gloves

Phosphorylethanolamine Skin & eye irritant. Wear lab coat and nitrile gloves

PMSF "Harmful by inhalation, ingestion or skin adsorption" 4ul/reaction Low Wear lab coat and nitrile gloves

Sink/sluice with copious amounts of water.

Poly-D- Lysine None Wear lab coat and nitrile gloves

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalPotassium chloride None 3g Low Wear lab coat and

nitrile glovesyellow bin

Puromycin Irritating to eyes & skin. Harmful by inhalation Wear lab coat and nitrile gloves

Saponin Low risk 1% soln Wear lab coat and nitrile gloves

Sodium hydrogen phosphate

None / low risk 2g none Wear lab coat and nitrile gloves

yellow bin

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalHydrocortisone Skin irritant. 1mg Wear lab coat and

nitrile gloves

Pokeweed mitogen skin irritant,carcinogen Wear lab coat and nitrile gloves

Brefelden A harmful by inhalation and skin contact Wear lab coat and nitrile gloves

CAPS low risk Wear lab coat and nitrile gloves

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalF-Met-leu-pro low risk Wear lab coat and

nitrile gloves

Ionomycin none Wear lab coat and nitrile gloves

Lt1,LT2 transfectine reagents

None Wear lab coat and nitrile gloves

Albumax - contains serum albumin

"Avoid inhalation, contact with skin & eyes" Wear lab coat and nitrile gloves

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalRapamycin, from Streptomyces hygroscopicus


1mg Low wear buttoned up lab coat and nitrile gloves


Ethynylestradiol, Beta-oestradiol & analogues

harmful by skin or eye exposure, and by inhalation. <1mg Low Wear lab coat and nitrile gloves

purple bin

Blasticidin S, Hydrochloride Streptomyces griseochromogenes

TOXIC Fatal if swallowed. 5g High wear buttoned up lab coat, gloves, mask and eye protection. Handle in fumeb hood and ensure hood working before use.

Via safety services

Doxycycline hyclate Harmful if swallowed.Causes skin, eye and resp tract irritation. 1g Medium wear lab coat, nitrile gloves and eye protection


Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalG 418 disulfate salt May cause an allergic skin reaction. May cause allergy or

asthma symptoms or breathing difficulties if inhaled.1g Medium wear lab coat, nitrile

gloves and face mask


DMSO Not a hazardous substance or mixture according to Regulation (EC) No. 1272/2008.

100ml Low Wear lab coat and nitrile gloves


FuGENE HD transfection reagent

Flammable WEL Long-term value: 1920 mg/m³, 1000 ppm 1ml Low Keep container tightly closed and in a well-ventilated place.Keep away from sources of ignition. Wear buttoned up lab coat and nitrile gloves

Down sink with copious amounts of water

TurboFect in vitro Transfection Reagent

None 100ml Low Wear lab coat and nitrile gloves



Carbenoxolone Harmful if swallowed; irritating to eyes Wear lab coat and nitrile gloves

Chlorocelline * "Harmful by inhalation, or skin adsorption if used in large quantities. Possible teratogen"

Wear lab coat, nitrile gloves and eye protection

Chloroquine * "Toxic by inhalation, in contact with skin if used in large quantities.Possible irriversible effects, teratogen."

100uM/500mls Wear lab coat, nitrile gloves and eye protection

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalCitral "Harmful: causes severe irritation, may cause sensitisation by

skin contact."Wear lab coat and nitrile gloves

Collagenase None Wear lab coat and nitrile gloves

Concanavalin A (Con A) *

Harmful in contact with skin and by inhalation, carcinogen Wear lab coat and nitrile gloves

Phorbol 12-myristate acetate (PMA) *

Skin and respiratory irritant 500ng/ul Low Wear lab coat and nitrile gloves

yellow bin

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalCrystal violet None / low risk Wear lab coat and

nitrile gloves

Cytokines & growth factor reagents from various sources

Biohazard cant be generalised Low Wear lab coat and nitrile gloves

yellow bin

Tetraethylthiuram disulphide (Disulfiram)

"Toxic by inhalation, in contact with skin & if swallowed.Irritating to all sites. Irriversible effects. "

Wear lab coat and nitrile gloves

NaCl low risk /no risk <4g Low Wear lab coat and nitrile gloves

Orange bin of sink/sluivce with copious amounts of water.

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalLPS harmful by inhalation, skin contact,pyrogen 10ug/ul Low Wear lab coat and

nitrile glovesOrange bin

PHA-Leucoagglutinin harmful by ingestion,inhalation and skin absorption. 500ng/ml Wear lab coat and nitrile gloves

P-Nitrophenol-N-Acetyl-B-D-Glucosamine

maybe harmful by inhalation,ingestion or skin absorption. Eye and skin irritant.

<1g Wear lab coat and nitrile gloves

Fugene none/low risk 0.6ul/96well Wear lab coat and nitrile gloves

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalZeocin Harmful by ingestin and if comes into contact with skin 125mg stock Low Wear lab coat and

nitrile glovesSink/sluice with copious amounts of water.

Polyinosinic-polycytidylic acid (Poly1:C)

due care. Properties have not been thoroughly investigated 10ul Wear lab coat and nitrile gloves

R848-Resiquinnol none 500ug Wear lab coat and nitrile gloves

Ascorbic acid Non-hazardous 10g Wear lab coat and nitrile gloves

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalButyric acid, sodium salt,Sodium Butyrate

Non-hazardous 1mM Wear lab coat and nitrile gloves

Dibutyryl cAMP Non-hazardous <0.5g Wear lab coat and nitrile gloves

Effectene transfection reagent

Low-risk 1ml Low Wear lab coat and nitrile gloves

Orange bin

FuGENE 6 transfection reagent

Low-risk <20ul Wear lab coat and nitrile gloves

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalHyaluronidase Non-hazardous <0.5g Wear lab coat and

nitrile gloves

Ionomycin Very toxic, harmful if swallowed <1mg Wear lab coat and nitrile gloves

Pepsin �Non-hazardous <50mg Wear lab coat and nitrile gloves

Pertussis toxin Highly toxic by inhalation, skin contact and if swallowed <50ug Wear lab coat and nitrile gloves

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions Disposalp-hyroxybenzoic acid (Methyl 4-hydroxybenzoate)

Irritating to eyes respiratory system and skin <10g Wear lab coat and nitrile gloves

Polybrene (hexadimethrine bromide)

Harmful by inhalation, ingestion and contact with shin <0.5g Wear lab coat and nitrile gloves

Beta-amyloid (1-42) peptide

respiratory irritant <1mg Wear lab coat and nitrile gloves

Prion peptide(106-126) respiratory irritant <1mg Wear lab coat and nitrile gloves

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalIL17 none unless injected 50ug/experiment Wear lab coat and

nitrile gloves

Actinomycin D skin, eye, respiratory irritant 10ug/ul Low Wear lab coat and nitrile gloves


ALLN in solution skin, eye, respiratory irritant; wear gloves few ul Wear lab coat and nitrile gloves

Thalidomide Teratogen and mutagen. Risk of heritable genetic damage, may cause congenital malformation in the fetus. Harmful by skin absorption. Eye and skin irritant

100ug/ml Wear lab coat and nitrile gloves

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions Disposalaphidicolin harmful if swallowed,inhaled or absorbed by the skin 0.1mg/l Wear lab coat and

nitrile gloves

Amaxa nucleofector kits none 50ng/ul Low Wear lab coat and nitrile gloves

yellow bin

fibronectin none 50ug/ul Low Wear lab coat and nitrile gloves

yellow bin

jet-pei low 10ug/ul Low Wear lab coat and nitrile gloves

yellow bin

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalNanofectin Not classed as hazardous 1ml Low Wear lab coat and


Sodium dihydrogen phosphate

irritant 1g Low Wear lab coat and nitrile gloves


Magnesium chloride irritant 1g Low Wear lab coat and nitrile gloves


Calcium Phosphate transfection kit


Orange bin

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalTfx 50 reagent Low-risk 1ml Low Wear lab coat and


Plasmid DNA Treat as suspect pathogens. 1ml Low Wear lab coat and nitrile gloves


Carrier DNA - Salmon Sperm DNA

May be harmful by inhalation, ingestion, or skin absorption. 1ml Low Wear lab coat and nitrile gloves

Orange bin

Polyethylenimine 50% solution



Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalThiamine Hydrochloride Low-risk 1ml Low Wear lab coat and


Transfast (Promega) None identified <1ml Low Wear lab coat and nitrile gloves

Orange bag / sink

TransIT (Mirus) none identified <1ml Low Wear lab coat and nitrile gloves

Orange bag / sink

Luciferin (Geneglow kits) None identified <1ml Low Wear lab coat and nitrile gloves

Orange bag /sink

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalOptison None identified <5ml Low Wear lab coat and

nitrile glovessink / orange bag

Echovist None identified <5ml Low Wear lab coat and nitrile gloves

orange bag / sink

Potassium ferricyanide/ferrocyanide

Harmful <10g Low Wear nitrile gloves & lab coat. Avoid inhalation


Nonident P-40 Irritant 100ml Low Wear nitrile gloves & lab coat


Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalSodium deoxycholate Harmful <100g Low Wear nitrile gloves &

lab coatSink with copious amounts of water.

LPS (lipopolysaccharide) Harmful Low Wear lab coat and nitrile gloves Use in class II hood.


MALP 2 Toxic 50ug Low Wear lab coat and nitrile gloves Use in class II hood.


E5564 (TLR4 agonist) Hazards unknown. 7mg Low Wear lab coat and nitrile gloves Use in class II hood.


Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalKrebs-Henseleit Buffer None 100ml stock, 2ml

per sampleLow Wear lab coat &

nitrile glovesSink with copious amounts of water

Luminol Irritant 100ml stock Low Wear lab coat & nitrile gloves


Cathepsin D Activity Assay Kit (Fluorometric)

Cell lysis buffer causes Serious eye damage/eye irritation 1 kit Medium wear gloves and eye protecetion


LysoSensor™ Yellow/Blue DND-160 (PDMPO) *1 mM solution in DMSO*

Components of the product may be absorbed into the body through the skin

100ml Medium wear gloves down sink with copious amts of water

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalMG132 None 5mM in DMSO Low Wear nitrile gloves &

lab coatSink with copious amounts of water

Adenosine 5' triphosphate peroxidate oxidised sodium salt

Possible irritant 100um Low Wear nitrile gloves & lab coat


A438079 Hydrochlorine Irritant. Harmful >10mM Low Wear nitrile gloves & lab coat Avoid inahalation and repeated exposure. Use in a fume hood.


SiRNA'S None 5nMol Low Wear nitrile gloves & lab coat

Sink with copious amounts of water. SiRNA transfected cells should be treated /inactivated prior to disposal

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalSiRNA buffer (contains 300mM HEPES, 1mM MgCl2)

None 100ml stock Low Wear lab coat & nitrile gloves


Dharmafect None 1.5ml Low Wear lab coat & nitrile gloves

Sink with copious amounts of water. Transfected cells should be treated / inactivated prior to disposal.

Ginkolide B Not hazardous 10mg Low Wear lab coat, eye protection & nitrile gloves. Avoid prolonged or repeated contact Hygiene measuresGeneral industrial hygiene practice

Dilute solutions down sink with copious amounts of water

Platelet activating factor Not hazardous 1mg Low Wear lab coat, eye protection & nitrile gloves. Avoid prolonged or repeated contact Hygiene measuresGeneral industrial hygiene practice

Dilute solutions down sink with copious amounts of water.

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalCNQX disodium salt irritant to eyes, skin, mucus membranes and respiratory tract 500ml Low wear lab coat, nitrile

gloveas and eye protection


Kainic Acid irritant to eyes, skin, mucus membranes and respiratory tract 500ml Low wear lab coat, nitrile gloveas and eye protection


Flunarizine dihydrochloride

Harmful if swallowed. 5g Medium wear lab coat, nitrile gloveas and eye protection Wear face mask when handling powder. Do not breath dust

Via safety services or specialised disposal compnay. Do not allow to enter drains

ISOGINKGETIN The chemical, physical and toxicological properties of this product have not been fully investigated.

5g Medium wear lab coat, unpowdered gloves, eye protection and face mask when handling. Do not breath dust. Do not get in eyes, on skin or clothing. Aviod repeated exposure

Do not allow to enter drains. Dispose of vis safety services or specialised company.

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalChlorhexidine diacetate salt hydrate

Harmful if swallowed.Very toxic to aquatic life with long lasting effects.

5g High Use in fume hood. Ensure hood is working before use with vanometer. Wearnitrile gloves, lab coat and eye protection

Via safety services or specialised disposl company. DO NOT allow to enter drains

Clotrimazole Harmful if swallowed.Causes skin irritation.Causes serious eye irritation.

5g Low wear lab coat and nitrile gloves


Chelerythrine chloride may be an irritantHarmful if absorbed through the skinHarmful if swallowedMay cause headache and drowsinessMay be fatal if inhaled

100mg Medium wear buttoned lab coat,nitrile gloves and eye protection. Use in fume hood. Ensure hood is working using vanometer before use.

down sink with copious amouts of water

ExGen 500 In Vitro Transfection reagent

Not considered dangerous according to EU directive 199/45/EEC

500ml Low wear buttoned up white coat & nitrile gloves




Assessed Sue Justice Date 31/01/2013


Approved by: Date


Phospholipid compoundsNone 2.5mg/ml Low wear lab coat, nitrile

gloveas and eye protection

Orange Bin

Tubastatin A (trifluoroacetate salt)

Chemical, physical, and Toxicological properties have not been thoroughly investigated. Product should be considered hazardous until further information becomes available.

1mg Medium wear lab coat, nitrile gloves, eye protection and face mask when handling. Do not breath dust

Yellow box bin



Tracking No: 7 Labs Involved: All staff & field

Title of Procedure: Use of liquid nitrogen & dry ice

Brief Description:The filling and maintenance of storage vessels contaning liquid nitrogen from reservoir tanks or dewars. Keeping samples on dry ice or liquid nitrogen during an experiment to maintain them at very low temperatures during sample collection or usage. Posting samples on dry ice


Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalLiquid Nitrogen Asphyxiant in high concentration. Causes burns & severe

frostbite on contactUpto 25litres High Use in well

ventilated room which an oxygen depletion meter installed.Never use whilst on own. Use cryoprotective gloves with elasticated cuffs, visor, and stout shoes. Use tongs to lower sample into

By evaporation in fume hood

Dry ice Asphyxiant, causes severe burns on contact with skin. 2kg High Use in well ventilated room which an oxygen depletion meter installed.Never use whilst on own. Use cryoprotective gloves with elasticated cuffs,

By evaporation in fume hood


Use in a well ventilated area with a functioning oxygen depletion monitor. Wear padded cryo- gloves & face shield/visor when replenishing liquid nitrogen/dry ice or handling stored containers. Possible explosive danger from sample tubes where these may contain liquid nitrogen. Use screw capped cryovials where possible.

Assessed Daniel Fillingham Date 19/10/2012


Approved by: Date




Title of Procedure: Agarose gel electrophoresis of RNA and DNA

Brief Description:Agarose gel electrophoresis is used for the separation of DNA/RNA fragments ranging from 100bp to 10kb. Agarose is melted into one of several buffers, ethidium bromide is added and the mixture is allowed to set before being loaded with RNA or DNA samples.

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions Disposal"TBE - Tris, Boric acid, EDTA" 0.04M

1) Irritating to eyes, respiratory system and skin. 2) Causes eye and skin irritation. 3) Material is irritating to mucous membranes and upper respiratory tract.

50-100ml Low Good Laboratory Practise (GLP)

Agarose 0.4-2.5% Eye: May cause eye irritation. Dust may cause mechanical irritation. Skin: May cause skin irritation. May be harmful if absorbed through the skin.Ingestion:t. May be harmful if swallowed. May cause resp tract irritation

0.5g-3.75g Low GLP. Agarose needs to be heated in a microwave to dissolve. Wear thermal gloves when handling molten solution as they can become superheated

DNA ladder None 10-20ul Low GLP

Ethidium bromide solution

Mutagenic. Irritant to skin and eyes 10ug/ml 10ml. <10ul used per gel

Medium GLP. Wear dust mask and weigh out powder in a fume-hood

1ug-.5mg/ml use actvated charcoal method; >.5mg/ml dilute to .5mg/ml - use chemical method (KMnO4, HCl, NaOH)

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalSample / loading buffer None 5ul Low GLP

TAE(Tris,acetic acid,EDTA)


30ml-1.5ml Low GLP

GelRed None Known 10,000x in water Low GLP

Tris-Acetate-EDTA buffer(TAE) 10x


500ml of 1X solution Low GLP

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalTris-Borate-EDTA (TBE) 10x



MOPS-EDTA-Sodium Acetate Buffer (MESA)



37% Formaldehyde Highly toxic, Severe irritant 0.9ml of 37% Solution

Medium GLP. Dispense in fume cupboard.

RNA sample loading buffer containing Formamide,Formaldehyde and Methyl Alcohol

Toxic 5ul Low GLP. Use in well ventilated area.

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalTris 1) Irritating to eyes, respiratory system and skin. 2) Causes

eye and skin irritation. 3) Material is irritating to mucous membranes and upper respiratory tract.

500g Low GLP

Glycine 1)May irritate skin and eyes 2) May be harmful if ingested in quantity.

150g Low GLP

EDTA 1) Irritating to eyes, respiratory system and skin. 2) Causes eye and skin irritation. 3) Material is irritating to mucous membranes and upper respiratory tract.

150g Low GLP

Borate 1)Irritating to eyes, respiratory sytem and skin. 2) Material is irritating to mucous membranes and upper respiratory tract

500g Low GLP

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalBromophenol blue 1)May be harmful by ingestion 2) Stains 3) May irritate eyes <0.1g Low GLP

Activated charcoal Harmful if inhaled. Irritant to skin, eyes and respiratory system 20g Low GLP and eye protection

Any activated charcoal that has been in contact with buffer containing ethidium bromide (and the filter paper) to be disposed of in orange bag (clinical waste)

RNA and DNA biohazard - potential immunogen 2-100ug GLP

Diethyl pyrocarbonate Causes respiratory tract irritation. Irritant. Causes eye and skin irritation. May be absorbed through intact skin.

20mL Low GLP and eye protection



Assessed Sarah Waite Date 16/10/2012


Approved by: Date

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalAgilent RNA 6000 Nana kit

All contents considered non-hazardous apart from nano dye concentrate (contains DMSO), this should be considered potentially damaging to skin and eyes

1 kit Low GLP




Title of Procedure: Polyacrylamide gel electrophoresis

Brief Description:An electric current is passed through the polyacrylamide gel, the DNA fragments migrate according to their size and so the DNA can be separated, identified and purified. This technique is also used for the separation of proteins in 2D electrophoresis.

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions Disposal"N,N,Ní,Ní-tetramethylethylenediamine (TEMED)"

1) Highly flammable 2) Corrosive: cause burns 3) Harmful by inhalation and if swallowed 4) can react violently with oxidising materials

30ul <200ul 100ml stock

Medium Good laboratory practice (GLP) Wear dust mask and weigh out powder in a fume-hood use in fumecupboard if one ia available.

"TAE - Tris, Sodium acetate, EDTA"

Irritant ot eyes and skin 1L 10x diluted to 1x 1L 50X dilute to 1X

Low GLP

"TBE - Tris, Boric acid, EDTA" 10x TBE

Irritant ot eyes and skin 10x TBE<100ml 1L stock

Low GLP

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalAcrylamide 1)Cumulative neurotoxin 2) Potent Carcinogen 3) Mutagen /

Teratogen 4) Toxic: danger of serious damage to health by prolonged exposure through inhalation, in contact with skin and if swallowed. Long ter exposure limit 0.3mg/m3

per 10ml vol.0.25ml 30% solution

High GLP. Weigh out in fume cupboard.

Absorb liquids on an inert absorbant and transfer to a sealed container.

Amberlite Eye irritant. May cause skin irritation. May cause irritation of the digestive tract.

<0.5g 500g stock Low GLP

Ammonium Persulfate 1)Harmful if swallowed 2) Irritating to eyes respiratory system and skin 3) May cause sensitization by inhalation 4) oxidising 5) Contact with combustable material may cause fire.

1g 10% w/v Stock of 50g

Low GLP

Isopropanol Flammable Use 1-2ml. Stock 2.5L Long term exposure - 999mg/m

Low GLP Absorb on to paper and allow to evaproate in fume cupboard.

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalBisacrylamide "Neurotoxin & carcinogenic, irritant,accumulative effects by

inhalation and skin or eye contact"1l Stock soln (1%) 1-20ml used

High GLP. Weigh out in a fume cupboard.

Absorb liquids on an inert absorbant and transfer to a sealed container.

Butanol "Flammable, harmful by inhalation" <5ml used. 500ml stock

Low GLP. Use in a well ventilated area.

Absorb onto inert absorbant material and allow to evaporate in fumecupbaord

Repelcote VS (contains 2%Dimethyldichlorosilane & >90% octamethylcyclotetrasiloxane)

"Flammable, harmful by ingestion, inhalation and skin contact, eyes. Reacts violently with water to produce HCl"

~1ml used. 500ml stock

Low GLP. Use in a fumecupboard. Do not mix with water.

Absorb on to inert absorbant material. Allow ot dry in fumecupboard.

DNA ladder no risk 5ul. Stock of 500ul Low GLP

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalEthidium bromide solution 10mg/ml

Mutagenic. Irritant to skin and eyes 10ug/ml 10ml. <10ul used per gel

High GLP. Wear dust mask and weigh out powder in a fume-hood

1ug-.5mg/ml use actvated charcoal method; >.5mg/ml dilute to .5mg/ml - use chemical method (KMnO4, HCl, NaOH)

Glycerol (less than 50ml) May cause eye, skin, and respiratory tract irritation. 2.5l Low GLP

Glycine (less than 1kg) 1) May irritate skin and eyes 2) may be harmful if ingested in quantity. May be absorbed through intact skin.

150g Low GLP

ethanol Flammable. Long ter exposure limits 1000ppm or 1920mg/m3 Causes severe eye irritation.

<5ml used in procedure. 2.5L stock

Low GLP Allow to evaporate.

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalSodium dodecyl sulphate (SDS)

1) Harmful if inhaled of swallowed 2) May be harmful if absorbed through the skin 3) causes severe eye irritation 4) Causes skin irritation 5) Material is irritating to mucus membranes and upper respiratory tract. 6) Risk of serious damage to eyes

10.0% (w/v) Medium GLP. Wear dust mask when weighing out powder. Weigh out powder in a fume-hood

sucrose May cause eye and skin irritation 5kg Low GLP. Wear dust mask when weighing out powder. Weigh out powder in a fume-hood

Urea "Irritating to eyes, respiratory system & skin." 75g used per gel. 1kg stock

Low GLP. Take care when warming up to dissolve in to solution. Do not allow to boil.

Precison plus std Not hazardous 1 kit Low GLP

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalGelatin May cause eye and skin irritation. <5g Low GLP

Methanol Flammable. Short term exposure 250ppm or 330mg/m3. Long term exposure 200ppm 0r 366mg.m3

100ml used. 2.5L stock

Low GLP

Acetic acid Corrosive. Flammable. Causes burns. 2.5L stock <100ml used

Medium GLP. Use in fume hood. Avoid inhilation.

Orthophosphoric acid Harmful. Causes burns. 100ml Low GLP. Use in fume hood.

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalSodium hydroxide pellets Corrosive. Harmful. 250g stock <20g

usedMedium GLP and eye

protection

Protogel resolving buffer Not a hazardous substance or mixture according to Regulation (EC) No. 1272/2008

450ml Low GLP

Protogel Stacking Buffer Not a hazardous substance or mixture according to Regulation (EC) No. 1272/2008

200ml Low GLP

40% Polyacrylamide solution

Polyacrylamide – Toxic in contact with skin and if swallowed or by inhalation, irritating to eyes and skin, may cause sensitisation by inhalation and skin contact, long-term WEL= 0.3mg.m3

Up to 20ml. 500ml stock solution

Medium GLP and eye protection.

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions Disposal10 x TBE – contains Tris, Boric Acid, EDTA

Tris/Boric acid/EDTA/APS– irritating to eyes, respiratory system and skin

Up to 50ml Low GLP

10% Ammonium persulphate (APS)

APS – contact with combustible material may cause fire, harmful if swallowed, may cause sensitisation by inhalation and skin contact

120ul sotck of 500ul Low GLP

Amplified DNA Tris/Boric acid/EDTA/bromophenol blue – irritating to eyes, respiratory system and skin

Bromophenol blue – harmful in contact with skin

Risk assessmentsLL5- use of high voltage equipment used for electropheresis

5ul Medium GLP

Precast polyacrylamide gel

May cause eye, skin, and respiratory tract irritation. Potential cancer hazard.

1 gel Low Good laboratory practice

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalDNA Ladders – PBR322/HaeIII and �x174/HaeIII - BioRad hyperladders

May cause eye and skin irritation. May cause respiratory and digestive tract irritation.

2-5ul used. 500ul stock

Low Good laboratory practice

Loading dye – contains Bromophenol blue (0.025g) and glycerol

May cause eye and skin irritation. May cause respiratory and digestive tract irritation.

5ul used. 10-20ml stock


4% polyacrylamide gel (See COSHH form 572)

4% polyacrylamide gel (See COSHH form 572) 1l Medium Good laboratory practice

Novex® Sharp Unstained Protein Standard

not hazardous 5ml Low GLP

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalProtein extracts from human tissues

Potential Biohazard Varies Low GLP

Tris 1) Irritating to eyes, respiratory system and skin. 2) Causes eye and skin irritation. 3) Material is irritating to mucous membranes and upper respiratory tract.

500g Low GLP

Beta Mercaptoethanol Highly toxic, do not breathe vapour, avoid contact with skin & eyes" Evidence of mutagenic effects Combustable

150ul of 14M <500ul Medium GLP. Decanting from stock should be done in a fume cupboard Warn allergic colleagues BEFORE use

EDTA 1) Irritating to eyes, respiratory system and skin. 2) Causes eye and skin irritation. 3) Material is irritating to mucous membranes and upper respiratory tract.

150g Low GLP

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalBromophenol blue 1)May be harmful by ingestion 2) Stains 3) May irritate eyes <0.1g Low GLP

Dithiothreitol(DTT) Harmful if swallowed, irritant to eyes and skin. 1g Low GLP

Rhinohide (polyacrylamide gel strengthener)

Unknown- not fully tested assume to be harmful 40ml Low GLP

Iodoacetamide Toxic if swallowed. Irritant to eyes, respiratory system and skin. May cause sensitisation by inhalation or contact with skin.

3.5g Low GLP

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalBrillant Blue G-collodial conc.

irritant to skin and eyes 20% Low GLP

Amplify Reagent HARMFUL. May cause burns 1L Low GLP and eye protection

HCl Causes burns. Irritating to respiratory system. 2.5l Medium GLP

Gel Code Blue Stain, contains acid, methanol and dimethyl sulphoxide

TOXIC by inhalation, ingestion, eye and skin splashes. May impair female fertility.

500ml Medium GLP and eye protection. USE IN A FUME HOOD. Do not breathe fumes, Keep container tightly closed and store away from heat.



None

Assessed Sarah Waite Date 16/10/2012


Approved by: Date

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalCoomassie Brilliant Blue R

Not classified as hazardous according to directive 67/548/EEC 500ml Low GLP




Title of Procedure: Preparation of various buffer solutions

Brief Description:constituents of common buffers

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalBoric acid "Avoid contact with eyes & skin, causes irritation" <100g Medium Wear unpowdered

nitrile gloves, lab coat and eye protection

Dilute solutions down sink with water

Hydrochloric Acid 0.1-4.0M

Toxic, corrosive-causes burns. Exothermic on contact with water

500ml Medium Wear unpowdered nitrile gloves, lab coat and eye protection

Dilute solutions down sink with plenty of water.

Glucose Eye irritant <50g Low wear unpowdered nitrile gloves


Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalFormamide Teratogen. Female workers must be considered potentially

pregnant. Possible carcinogen. Irritant. May cause severe burns and blistering which may not be immediately evident or painful.

<10g High Wear white coats, unpowdered nitrile gloves and eye protection.

Via safet services waste chemical route.

Glycerol None <100ml Low minimise contact, wear unpowderd nitrile gloves

down sink with copious amounts of water.

Glycine Not hazardous according to Directive 67/548/EEC. 100g Low minimise contact, wear unpowderd nitrile gloves


Magnesium chloride Substances to be avoided include most common metals, strong oxidizing agents. Protect from moisture. When mixed with water heat generated may be sufficient to cause frothing. Eye and skin irritant.

<10g Low Wear unpowdered nitrile gloves and eye protection.

Down sink with copious amounts of water.

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalMOPS (3(N-Morpholino)propanesulfonic acid)

Very hazardous in case of eye contact (irritant). Hazardous in case of inhalation. Inflammation of the eye ischaracterized by redness, watering, and itching.

100g Medium Wear unpowdered nitrile gloves, white coat and eye protection.


N-2-Hydroxyethylpiperazine-Ní-2-ethensulphonic acid (HEPES) up to 200mM

Hazardous in case of ingestion. Slightly hazardous in case of skin contact (irritant), of eye contact (irritant), ofinhalation.

100g Medium Wear unpowdered nitrile gloves, white coat and eye protection


Orange G "Avoid contact with skin & eyes, causes irritation, do not inhale dust"WARNING! TOXICOLOGICAL PROPERTIES UNKNOWN. MAY BE HARMFUL IF SWALLOWED, INHALED OR ABSORBED THROUGH SKIN. MAY CAUSE IRRITATION TO SKIN, EYES, AND RESPIRATORY TRACT.

100g Medium Wear unpowdered nitrile gloves, lab coat and eye protection


Potassium chloride Irritant to eyes & respiratory system if inhaled as dust. Incompatible with strong oxidizing agents, strong acids. Presents a low toxicological hazard. Ingestion of large quantities may be harmful.

1kg Low none down sink with copious amounts of water

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalProteinase K Hazardous in case of inhalation (lung irritant, lung sensitizer).

Slightly hazardous in case of skin contact (irritant,sensitizer), of eye contact (irritant), of ingestion, .

100g Medium Wear lab coat, nitrile gloves and eye protection, avoid inhaling dust-use in fume hood and ensure hood working before use with a vanometer

Down sink with copiuous volumes of water

Sodium acetate "Avoid contact with eyes & skin, do not inhale dust." Not hazardous according to Directive 67/548/EEC. Incompatible with strong oxidizing agents, halogens

1kg Low wear lab coat and unpowderd nitrile gloves

down sink with copious volumes of water

Sodium chloride Incompatible with strong oxidizing agents. May cause eye irritation.

1kg Low wear lab coat down sink with copious volumes of water.

Sodium Dodecyl sulphate (SDS)

Irritant to eyes & respiratory system if inhaled as dust. Harmful if swallowed, inhaled or absorbed through the skin.Nuisance dust, but may also cause sensitization if inhaled.Incompatible with strong acids, strong oxidizing agents. Hygroscopic. Flammable

1kg Medium Wear lab coat, unowdered nitrile gloves and eye protection.


Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalSucrose Combustible. Incompatible with strong oxidizing agents.

Inhalation of powder may cause respiratory irritation.1kg Low Wear lab cat and

unpowderd nitrile gloves

Down sink with copious volumes of water

Tricine Hazardous in case of ingestion. Slightly hazardous in case of skin contact (irritant), of eye contact (irritant), ofinhalation.

1kg Medium Wear lab coat, unpowdered nitrile gloves and eye protection

Down sink with copious volumes of water.

Tris - HCL WARNING! HARMFUL IF SWALLOWED. MAY CAUSE IRRITATION TO SKIN, EYES, AND RESPIRATORY TRACT.

1kg Medium Wear lab coat, nitrile gloves and eye protection, avoid inhaling dust-use in fume hood and ensure hood working before use with a vanometer


Tris-(hydroxymethyl) methylamine

Combustable. Produces toxic fumes in fire. Harmful if ingested. Irritant to eyes.

1kg Medium Use in fume hood. Wear unpowdered nitrile gloves and eye protection.

Down sink with plenty of water.

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalTriton X-100 Hazardous in case of eye contact (irritant), of ingestion, of

inhalation. Slightly hazardous in case of skin contact (irritant). Possible teratogen/mutagen. Femails must be considered to be pregnant.

100ml Medium wear unpowdered nitrile gloves, lab coat and eye protection

down sink with copious volumes of water.

Tween 20 Not hazardous according to directive 67/548/EC. Incompatible with strong oxidizing agents.

100ml Low Minimise exposure-wear lab coat and unpowdered nitrile gloves


Xylene cyanol ff "Do not inhale dust, may irritate eyes" Very hazardous in case of skin contact (irritant), of eye contact (irritant). Liquid or spray mist may produce tissue damage particularly on mucous membranes of eyes, mouth and respiratory tract.

100g High Wear lab coat, nitrile gloves and eye protection, avoid exposure-use in fume hood and ensure hood working before use with a vanometer

Via safety services waste chemical route

PBS tablets Under normal circumstances and use, this product is unlikely to cause any health hazard.

100 tablets Low wear unpowdered nitrile gloves


Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalAmmonium carbonate Harmful if swallowed. Eye, skin and respiratory irritant. May be

harmful if inhaled. Incompatible with strong acids, nitrates, nickel, copper

1kg Medium Minimise exposure-wear lab coat and unpowdered nitrile gloves


ammonium sulphate Contact with strong oxidizers may cause fire or explosion. Incompatible with strong bases. Not hazardous according to Directive 67/548/EEC.

1kg Low Minimise exposure-wear lab coat and unpowdered nitrile gloves


potassium carbonate Incompatible with moisture, acids, magnesium bromine trifluoride and magnesium bromine trichloride. Harmful if swallowed. May seriously irritate respiratory system and eyes. Skin irritant.



Potassium sodium tartrate

May cause irritation to the respiratory tract. Irritant to eyes and skin Large oral doses may cause irritation to the gastrointestinal tract. May cause sore throat, abdominal pain. Large doses can have cathartic effects.



Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalAcetic acid(glacial) This material is strongly corrosive and causes serious burns.

Very harmful if swallowed. Lachrymator.2.5L Medium Wear lab coat, nitrile

gloves and eye protection, avoid exposure-use in fume hood and ensure hood working before use with a vanometer

Down fume hood sink with copious volume sof water

Bromophenol blue May cause irritation to the respiratory tract. Symptoms may include coughing and shortness of breath. Effects not determined. Large oral doses may cause irritation to the gastrointestinal tract. Irritation to eyes and skin with redness and pain

80mg Medium Wear lab coat, nitrile gloves and eye protection, avoid exposure-use in fume hood and ensure hood working before use with a vanometer

Down sink with copious volume sof water

Magnesium chloride hexahydrate

Eye and skin Irritant. Harmful if ingested. <10g Low Wear unpowdered nitrile gloves and eye protection


Calcium chloride dihydrate

Eye and skin Irritant. Harmful if ingested. 10G Low Wear unpowdered nitrile gloves and eye protection


Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalEDTA Eye irritant. <20g Low Wear unpowdered

nitrile gloves and eye protection


Magnesium sulphate Dust may be slightly irritating. Sore throat or coughing may occur. Ingestion-Since magnesium salts are slowly absorbed, abdominal pain, vomiting and diarrhea may be the only symptoms

<5g Medium Wear unpowdered nitrile gloves and eye protection


Potassium acetate Avoid contact with eyes and skin, do not inhale dust <200g Medium Wear unpowdered nitrile gloves and eye protection


Sodium borate WARNING! HARMFUL IF SWALLOWED, INHALED OR ABSORBED THROUGH SKIN. CAUSES IRRITATION TO SKIN, EYES AND RESPIRATORY TRACT.



Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalSodium carbonate Incompatible with strong acids, aluminium, fluorine, lithium,

phosphorus oxides. Irritant. May be harmful if swallowed.20g Medium Wear unpowdered

nitrile gloves and eye protection


Sorbitol Hazardous in case of eye contact (irritant), of ingestion. Slightly hazardous in case of skin contact (irritant), ofinhalation.



Di-sodium tetraborate (borax)

Eye and skin irritant. Harmful if ingested. Long term WEL= 1mg/m3

<10 g Low Wear unpowdered nitrile gloves and eye protection

Dilute solutions down sink with water.

Citric acid Eye and skin irritant. Harmful if ingested. <10 g Low Wear unpowdered nitrile gloves and eye protection


Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalSodium hydrogen -orthophosphate

Eye and skin irritant. Harmful if ingested. <10g Low Wear unpowdered nitrile gloves and eye protection


Sodium dihydrogen -orthophosphate



Potassium dihydrogen -orthophosphate



Sodium phosphate Eye and skin irritant. Harmful if ingested. <10g Low Wear unpowdered nitrile gloves and eye protection


Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalDi-potassium hydrogen orthophosphate

Eye and skin Irritant. Harmful if ingested. <10g Low Wear unpowdered nitrile gloves and eye protection

Dilute solutions down sink with water

Potassium dihydrogen phosphate

Under normal circumstances anduse, this product is unlikely tocause any health hazard.

<500g Low Wear lab coat and unpowderednitrile gloves

Down sink withcopious volumes of water

Assessed Ian Coldicott Date 31/10/2012Approved by: Date




Title of Procedure: In-Situ hybridisation

Brief Description:

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalAcetic Anhydride "flammable, causes burns, avoid contact" 1ml Medium Wear gloves, Comply with local regs

Aminopropyltriethoxysilane

"Causes burns, avoid contact" 5ml Low Wear gloves, use in well ventilated area

Flush with copious water

Bromochloroindole phosphate (BCIP)

"Toxic by inhalation, ingestion & skin contact. Avoid contact with eyes and skin, causes irritation"

0.5ml Low Wear gloves, Flush with copious amounts of water

Dextran sulphate "May be Irritating to eyes, respiratory tract & skin" 100ug Low wear gloves Flush with copious amounts of water

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalEDTA "Avoid contact with eyes & skin, causes irritation" 2g Low Wear Gloves Flush with copious

amounts of water

Ethanol Flammable 500ml Low wear gloves Flush with copious amounts of water

Formamide "Risk of serious damage to eyes, do not breathe fumes, avoid contact with skin,"

200ul Medium Use with appropriate ventilation

Flush with copious amounts of water

NBT Harmful by ingestion 500ul, Low wear gloves Flush with copious amounts of water

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalTris-buffer None 250ml Low wear gloves Flush with copious

amounts of water

Phenol / chloroform / isoamyl alcohol

Toxic by inhalation and in contact with skin. Causesburns.Corrosive.

100ml Medium Laboratory coat, gloves and eye protection should be worn

Place in a sealed container and dispose via hazardous waste route

tRNA from Bakers Yeast Not a hazardous substance or mixture according to Regulation (EC) No 1272/2008This substance is not classified as dangerous according to Directive 67/548/EEC.


down sink with plenty of water

Heparin sodium salt, from porcine intestinal mucosa



down sink with plenty of water

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalCitric acid Causes skin irritation.

Causes serious eye damage.500g Medium wear buttoned up

lab coat, gloves and eye protection


DIG RNA Labeling Mixture,

Harmful by inhalation and if swallowed. slightly hazardous to water and ground

10x 40 ul Medium wear buttoned up lab coat and gloves


Protector RNAse inhibitor There is at present no information or indication of hazardous properties.

2000U Low wear buttoned up lab coat


SP6 RNA polymerase The product contains no substances which at their given concentration, are considered to be hazardous to health

1000U Low wear buttoned up lab coat


Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalMicro Bio-Spin® P-6 Gel Columns Tris Buffer

Not a hazardous substance or mixture according to Regulation (EC) No 1272/2008This substance is not classified as dangerous according to Directive 67/548/EEC.

Pack of 25 spin columns


Comply with local regulations / Offensive waste route

T3 RNA polymerase None 1000U Low wear buttoned up lab coat


Cyanine 3 Amplification reagent

Harmful if swalloed. Irritating to eyes, respiratory system and skin.

63mg Medium Avoid breathing dust/fume/gas/mist/vapours/spray.P280 Wear protective gloves/protective clothing/eye protection/face protection

Do not allow to enter sewers/ surface or ground water. Dispose of in hazardous waste stream.

Fluorescien Amplification Reagent

Harmful if swallowed. Irritating to eyes, respiratory system and skin.

1ml Medium Ensure good ventilation/exhaustion at the workplace.Prevent formation of aerosols. Wear lab coat, gloves and eye protection.

Do not allow to enter sewers/ surface or ground water. Dispose via hazardous waste route

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalAnti-Fluorescein-POD There is at present no information or indication of hazardous

properties.150ul Low Wear lab coat,

gloves and eye protection.


Anti-digoxigenin POD-conjugate

There is at present no information or indication of hazardous properties

150ul Low Wear lab coat, gloves and eye protection.

Offensive waste route.

phenol Toxic if swallowed. Toxic in contact with skin.Causes severe skin burns and eye damage. Fatal if inhaled. Suspected of causing genetic defects.May cause damage to organs through prolonged or repeated exposur

100ml High wear buttoned up lab coat, butyl gloves, eye protection and face mask. Handle in fume hood and ensure hood working before use using vanometer. Mop spillages up pace in selaed bottle and dispose of via

Via safety services or approved waste disposal company

Acetic Anhydride Flammable liquid and vapour. Harmful if inhaled. Harmful ifswallowed. Causes severe skin burns and eye damage.

500ml High Handle with gloves in fume hood. Wear eye protection

Via Safety Services

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalRNAse ZAP Not a hazardous substance or mixture according to Regulation

(EC) No. 1272/2008.1 spray Low non haz waste stream

Proteinase k Causes allergic reactions. Harmful by ingestion.

0.1mg Low Wear gloves Flush with copious amounts of water

Methyl Green Harmful by ingestion. Irritating to eyes and skin.

100ml Low Wear gloves Flush with copious amounts of water

DEPC Irritating to skin.Harmful if ingested 3ml Low Wear gloves, use appropriate ventilation


Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalRNA & DNA probes Irritating to skin.Harmful by ingestion

and inhalation50ul Low Wear gloves comply with local

regulations

Paraformaldehyde Harmful by ingestion. Irritating to eyes, skin and respiratory system.

0.5g Medium Wear gloves comply with local regulations

Anti-FITC Harmful by inhalation and ingestion 1-10ul Low Wear gloves flush with copious amounts of water

Magnesium chloride Harmful if ingested. Irritates skin 2g Low wear gloves Flush with copious amounts of water

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalDPX Harmful if swallowed. Irritating to eyes and skin 1ml Medium Wear gloves, Use

appropriate ventilation


Normal serum Harmful by inhalation and ingestion. 200ul Low Wear gloves Flush with copious amounts of water

DNase I, grade I Hazardous, skin and respiratory tract irritant 3000U/ml Low Wear gloves Flush with copious amounts of water

DAB substrate Toxic, potential carcinogen 100ul Low Wear gloves Use disposal procedure described below

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalTriton X-100 - 0.1% in solution Low Wear gloves Flush with copious

amounts of water

Sodium citrate - 0.1% in solution Low Flush with copious amounts of water

Hydrogen Peroxide Can cause burns possible carcinogen, harmful 3mls Low Wear gloves Flush with copious amounts of water

DTT Harmful. Irritant <1g Low Wear gloves & lab coat

Sink

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalSodium Chloride None 1kg stock Low Wear gloves & lab

coatSink with copious amounts of water

Triethanolamine Harmful. Irritant 100ml Low wear gloves & lab coat


Denhardt's solution Harmful 1ml aliquots Low Wear gloves & lab coat

Sink / orange bag

Glycine Irritant 500g Low Wear gloves & lab coat


Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalButon-1-ol Harnful. Irritant 500ml Low Wear gloves & lab

coat. Use fume hood.

Large volumes - waste solvent drum Y. Small amounts - sink with copious amounts of water.

Apotag kit (contains potassium cacodylate)

Toxic. Potential mutagen & carcinogen <1ml components Low Wear gloves & lab coat

Sink / orange bag

Mercaptoethanol Harmful. Irritant. Mutagen 100ml stock <10ul used

Low Wear gloves & lab coat. Use a fume hood.


Roche blocking reagent (maleic Acid)

Ingestion may cause gastrointestinal irritation, nausea, vomiting and diarrhea, Contact with eyes may cause irritation, may cause sensitization

50g Low wear buttoned up lab coat and gloves




Wash affected surfaces with copious amounts of water. DAB disposal procedure:0.2M Pot Perm2.0M H2SO4

Assessed Lynne baxter Date 07/11/2012


Approved by: Date




Title of Procedure: Column chromatography and protein fractionation

Brief Description:Separartion of protein mixtures by a variety of chromatography techniques

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalB - mercaptoethanol imidasole

none 2ml

CN-Br activated sepharose

Flammable 5g

D T T None 100ug

E D T A None 10g

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalGlycerol none 20ml

Guanidine hydrochloride None 100g

Lectin None 20mg

Magnesium chloride None 10g

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalN T A - agarose none 5g

P B S None 500ml

Phenyl -sepharose Flammable 100ml

Polyethylene glycol None 100ml

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalPotassium chloride None 100g

Q-sepharose Flammable. 10ml

Reactive blue Sepharose Flammable 10ml

Sephacryl Flammable 300ul

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalSodium chloride None 100g

Sodium fumarate None 10g

Sodium hydroxide Causes severe burns . Avoid contact with skin 10g

Zinc chloride None 1g

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalTalon Metal affinity resin T - Toxic

N - Dangerous for the environment100ml High wear safety specs

and gloveshazardous waste stream

Carbonic anhydrase Non-hazardous <10mg

Duolite MB 6/13 mixed resin

Do not breath dust <5g

Iminodiacetic acid Sepharose

Flammable, irritating to eyes and skin <30ml

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalIsopropyl-B-D-thiogalactopyranoside

Irritant, do not braeth dust <1g

Protein standard gel filtration marker

Non-hazardous 10ul

Sephadex (various) Non-hazardous 20g

Profound Pull-down protein interaction kit - contains Cobalt chelate and Imidazole

None 500ul-5ml Low Wear gloves & lab coat

Orange bag / sink with copious amounts of water.



Assessed Dr J G SHAW Date


Approved by: Date



Tracking No: 13 Labs Involved: LU118

Title of Procedure: Experimental human colonisation with Neisseria lactamica

Brief Description:Selective Neisseria negative individuals.Inocluate volunteers with 10x4 Neisseria lactamica live bacteria.Monitor colonisation and carriage using tonsillar and nasopharyngeal swabs, plus normal saline gargles

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalNeisseria lactamica Group 2 pathogen 1ml of 10x8 Low Handle in a class 2

hood.Wear gloves.Deal with spillages immediately.

Autoclave

Normal saline No hazard 5ml per gargle nil None Autoclave

Phosphate Buffer Saline None 20mls None None Autoclave

Mucolysin, Dithiothreitol None 400mg None nil Autoclave

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalXgal= 5-Bromo-4-chloro-3-indolyl-Beta-D-galactopyranoside

None 20mg None None autoclave

Oracol Transport Medium=5%new borne bovine serum,0.1%brij plus PBS

Irritant to eyes.Harmful if swallowed. Skin irritant 500g Medium Wear suitable protection

Autoclave

N,N-dimethylformamide May casue harm to unborn child=tetragen.Also harmful by inhalation and in contact with skin. Readily absorbed through skin.Combustible liquid.

250ml High Wear protective clothing

Autoclave

NNN'N'N-Tetramethyl-p-phenylenediamine dihydrochloride

None 5g Low None Autoclave



Preparation of inoculum occurs in a designated Category 2 safety cabinet.All materials used in the preparation must be sterile. Pipettes are allocated specifically to this study only.The study protocol must be followed at all times.The inoculum will be monitored for purity by performing viable counts and xgal identification.

Assessed Ian Geary Date 23/01/2013


Approved by: Date



Tracking No: 14 Labs Involved: All Staff

Title of Procedure: The removal of contaminating DNA from RNA and the First Strand Synthesis of cDNA

Brief Description:DNase is used to treat RNA to remove any contaminating DNA prior to the synthesis of cDNA. cDNA is synthesised using the Superscipt II System.

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions Disposal"Advantage/GC CDNA PCR kit buffer (Clontech)� contains 1% DMSO, "

"DMSO - Irritating to skin & eyes, harmful by ingestion, penetrates skin"

3-5ul Low Good laboratory practice (GLP)

Ammonium acetate None in solution < 1ul Low GLP

AMV transcriptase None / low risk 200ul Low GLP

cDNA size fractionation columns

None / low risk 2-5ul Low GLP

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalControl RNA None / low risk 2-5ul Low GLP

Dithiothreitol Harmful by ingestion, Irritant to skin, eyes and respiratory tract < 1ul Low GLP

DNA ligase None / low risk <1ul Low GLP

DNA polymerase None / low risk <1ul Low GLP

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposaldNTP's None / low risk 2ul of 20mM stock Low GLP

EcoR I adapters None / low risk <1ul Low GLP

Ethanol Highly flammable <200ul Medium GLP. Decanting from stock should be done in a fume cupboard. Keep away from sources of ignition.

First strand / second strand / adapter / TEN buffers

May cause irritation to eyes & skin 3-5ul Low GLP

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalMagnesium Chloride None in solution 2ul of 50mM stock Low GLP

Phenol / chloroform / isoamyl alcohol

Highly toxic, harmful & corrosive to skin, eyes & respiratory tract

2ml Medium GLP and eye protection should be worn

Random hexamer primers & oligo dt primer

None / Low risk 100ng Low GLP

RNAse inhibitor, Ribonuclease H and Turbo Dnase

Low risk <2ul per reaction Low GLP

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalSuperscript II and MMLV reverse transcriptase

None / low risk 200u Low GLP

T4 DNA Ligase None / low risk <1ul Low GLP

T4 DNA polymerase None / low risk <1ul Low GLP

T4 polynucleotide kinase None / low risk <1ul Low GLP

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalTris HCL None in solution 20mM 2-3ul Low GLP

Yeast tRNA None / low risk <1ul Low GLP

Mineral Oil None/low risk 20ul Low GLP Mop up with absorbant paper

DIG-11-dUTP Harmful by inhalation and if swallowed. 25µL Low GLP

Chemical/Reagent Hazard Stock Volume/ Quant Risk level Precautions DisposalgDNA Wipeout Buffer, 7x Irritant to skin and mucous membranes.

Strong irritant with the danger of severe eye injury.100ul Medium GLP and eye

protection.Offensive waste route. Do not allow to enter sewer system.

RETROscript Kit low risk 40 tests Low GLP

Dna free kit low risk 40 tests Low GLP

Calf Intestine Phosphatase

low risk Low GLP

coshh assessment form - university of sheffieldmedicine.dept.shef.ac.uk/coshh2/pdfs/243.pdf ·...

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