A B

Germination rate was 100% and plant growth was normal in control application however germination rate and plant

growth were inhibited by salt or JA application.

Antioxydative enzymes, CAT, SOD and APX, increased with salt or JA treatments compared to control plantlets. The SOD, CAT and APX activities of the salt-tolerant Mardin variety were higher than salt-sensitive Kemer variety.

A B C

D E F

G H I

Effects of different applications for Mardin Kızıltepe

ConclusionsConclusionsAmong all applications of JA, plantlets that were pre-treated with 10 µM JA and then transferred to the salty media showed relatively better development than the other applications. As a result of our research, it is possible to claim that JA was perceived as a stress factor by the seedlings and JA applications resulted in enhanced enzyme activities. It can be even said that some doses of JA caused higher inhibition and more devastating effects on eggplant seedlings than salt applications did.

Salinity is a major abiotic stress reducing the yield of a wide variety of crops all over the world. Different strategies are being employed to optimize growth under saline conditions. One of them is to produce salt tolerant genotypes in different crops. In addition, many other attempts have been made to overcome this disorder, including exogenous application of plant growth regulators (1). Plant hormones are active members of the signal compounds involved in the induction of plant stress responses (2).Jasmonic acid (JA), which is naturally synthesized by plants, plays an important role as a signal molecule that induces the tolerance mechanisms under the influence of both biotic and abiotic stresses such as virus, bacteria and fungi infections, freezing, drought, heat and heavy metals. Pretreatment with JA reduced the inhibitory effect of high salt concentration on growth and photosythesis of barley (3).Eggplant (Solanum melongena L.), belongs to family Solanaceae, is an important crop for Turkey. According to the FAO 2009 statistics, Turkey with a yield of 297.000 tons in 30.000 hectares holds the first place in eggplant production in Europe. Furthermore, it is known that embryo culture applications in eggplant shorten the period of time needed for a generation for nearly 2 to 2,5 months.In this study we aimed to investigate the effects of JA individually and accompanied with salt stress on two cultivars of eggplant embryos cultured in vitro.

Effects of Jasmonic Acid and Salt Applications on Effects of Jasmonic Acid and Salt Applications on Antioxydative Enzyme Activities of the Eggplant Seedlings Antioxydative Enzyme Activities of the Eggplant Seedlings

CulturedCultured in vitroin vitro Banu GÜNALP1, Özge HORASAN1, Fikret YAŞAR2, Şebnem KUŞVURAN3, Rukiye TIPIRDAMAZ4, Şebnem ELLİALTIOĞLU1

1Ankara University, Faculty of Agriculture, Department of Horticulture, Ankara, Turkey2Yüzüncü Yıl University, Faculty of Agriculture, Department of Horticulture, Van, Turkey

3Çankırı Karatekin University, Kızılırmak Vocational High School, Çankırı, Turkey4Hacettepe University, Faculty of Science, Department of Biology, Ankara, Turkey

II. II. Materials and MethodsMaterials and Methods

I. I. IntroductionIntroduction

Embryos gently isolated from the 32 days old seeds of eggplants were transplanted on hormone-free MS (4,5). Medium basically containing 2% saccharose, 0.7% agar at pH 5.7 . Also different concentrations of NaCl and JA were applied. 9 different applications and two cultivars of eggplant were used: cv. Mardin (M: Tolerant) and Kemer (K: Sensitive) (6)

A.No JA and NaCl (14 days) C. JA 10 (10µM JA 14 days) E. NaCl control ( 100 mM NaCl 14 days) F. NaCl+JA 10(100mMNaCl+10µJA 14 days) H.Pre JA 10(10µM JA 4days)+NaCl (100mM NaCl 10 days) B. No JA and NaCl (14 days) D. JA 20 (20µM JA 14 days) G.NaCl+JA 20(100mMNaCl+20µJA 14 days) I. Pre JA 20(20µM JA 4 days)+NaCl (100 mM NaCl 10 days)

Embryos were incubated for 21 days at 25± 1 ⁰C and 16/8 hours of light/dark. After the incubation period, plantlets were harvested and germination percentage and antioxydative enzymes, SOD, CAT and APX, were determinated.

III. III. Results and DiscussionResults and Discussion

References 1.Kaya, C., Tuna, A.L., Yokaş, I. 2009. The Role of Plant Hormones in Plants Under Salinity Stress. İn: Salinity and Water Stress (Eds: Ashraf, M., Ozturk, M., Athar, H.R.), Springer Verlag. pp: 45-50. 2. Pedranzani, H., Racagni,, G., Alemano, S., Miersh, O., Ramirez, I., Pena-Cortes, H., Taleisnik, E., Machado-Domenech, E., Abdala, G. 2003. Salt tolerant tomato plants show increased levels of jasmonik acid. Plant Growth Regu. 41: 149-158.3. Tsonev, T.D., Lazova, G.N., Stoinova, Z.G., Popova, L.P. 1998. A possible role for jasmonic acid in adaptation of barley seedlengs to salinity stres. J Plant Growth Regul 17: 153-159.4. Ellialtıoğlu, Ş., & Öztürk, S. 1998. Embryo culture studies in eggplant (Solanum melongena L.). Biotechnological Industry University Cooperation Symposium (s. 33-38). Eskişehir: Anadolu University and the BiotechnologyAssociation.5. Murashige, T., Skoog, F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plantarum , 15: 473-497.6. Yaşar, F., 2003, Investigation of some Antioxidative Enzymes on Eggplant Genotypes Grown under Salt Stress in vivo and in vitro conditions . Yüzüncü Yıl University, Graduate School of Natural and Applied Sciences. (Ph.D. Thesis, in Turkish)), Van, Turkey,125p.