Journal of Fermentation and Bioengineering

C O N T E N T S

V O L U M E 74 • 1992

ORIGINALS

Gene Expression in Bacillus brevis under Control of the Lac Repressor-Operator System from Escherichia coli. Hiroaki Takagi, Hideo Yamagata, and Shigezo Udaka ......

Purification and Properties of the Phytase from Schwanniomyces castellii. Laurent Segueilha, Christel Lambrechts, H61~ne Boze, Guy Moulin, and Pierre Galzy ......

Overproduction of Thermostable fl-Galactosidase in Escherichia coli, its Purification and Molecular Structure. Takao Saito, Katsuya Kato, Sumio Maeda, Tohru Suzuki, Sumihisa Shiba, Shinji Iijima, and Takeshi Kobayashi . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Growth and Astaxanthin Formation of Haematococcus pluvialis in Heterotrophic and Mixotrophic Conditions. Makio Kobayashi, Toshihide Kakizono, Kazuya Yamaguchi, Naomichi Nishio, and Shiro Nagai . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Product ion of Artificial Seed from Horseradish Hairy Root. Nobuyuki Uozumi, Yutaka Nakashimada, Yoshihiro Kato, and Takeshi Kobayashi . . . . . . . . . . . . . . . . . . . . . . . . . . .

High Cell Density Cultivation of Anchorage-Dependent Cells using a Novel Macroporous Cellulosic Support. Tatsuya Ogawa, Masamichi Kamihira, Shuji Terashima, Kimiaki Yasuda, Shinji Iijima, and Takeshi Kobayashi . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Characterizing Acidification Kinetics by Measuring pH and Electrical Conductivity in Batch Thermophilic Lactic Fermentations. Eric Latrille, Daniel Picque, Bruno Perret, and Georges Corrieu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Neuro-Fuzzy Control of Bioreactor Systems with Pattern Recognition. Zhongping Shi and Kazuyuki Shimizu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Controlled Expression of Human Basic Fibroblast Growth Factor Mutein CS23 in Es- cherichia coil under a Bacteriophage T7 Promoter . Masato Kuriyama, Masanori Nakatu, Masafumi Nakao, Koichi Igarashi, and Kazuaki Kitano . . . . . . . . . . . . . . . . . . . . . . . .

Expression of the Ice Nucleation Active Gene of a Novel Plasmid pNVR-1 from Pseu- domonas viridiflava in Escherichia coli and Pseudomonas aeruginosa. Hideki Ohgama, Koji Izumi, Yoshie Hasegawa, Hitoshi Obata, and Tai Tokuyama .... . . . . .

Induction of Geotrichum candidum Lipase by Long-Chain Fatty Acids. Yuji Shimada, Akio Sugihara, Toshihiro Nagao, and Yoshio Tominaga . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Lysis of Rabbit ~'-Globulin-Coupled Liposomes by the Complement System. Masaaki Kishimura, Hideki Fukuda, Shigeo Katoh, Eizo Sada, and Hiroshi Taniguchi ......

Adsorption of Glucoamylase in Unpasteurized Sake on an Affinity Column of Immobi- lized Glucoamylase Inhibitors. Yoji Hata, Akitsugu Kawato, Yasuhisa Abe, Satoshi Imayasu, Kazuhisa Ono, and Satoru Oka . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Immobilization of Yeast Cells with Cross-Linked Chitosan Beads. Masa-aki Shinonaga, Yoshihide Kawamura, and Tsuneo Yamane . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Product ion of Propionibacterium freudenreichii subsp, shermanii in Whey-Based Media. Vincent Marcoux, Yvan Beaulieu, Claude P. Champagne, and Jacques Goulet ......

Photometric Measurement of High Cell Density by Continuous Dilution of Broth with a Circulating System. Takuo Yano, Agus Masduki, and Yoshinori Nishizawa ......

Factors Affecting Culture Fluorescence When Monitoring Bioreactors. Jian-Ke Li and Arthur E. Humphrey . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

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ii CONTENTS J. FERMENT. BIOENG.,

Thermal Control Strategy for Isoamylacetate Formation in Sake Brewed with a Sac- charified Rice Solution. Kazuo Matsuura, Masato Hirotsune, Masaaki Hamachi, and Yataro Nunokawa . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Stabilization of a Recombinant Plasmid in Yeast. Masato Kuriyama, Shigeru Morita, Naoki Asakawa, Masanori Nakatsu, and Kazuaki Kitano . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Prolyl Endopeptidase Inhibitory Activity of Peptides in the Repeated Sequence of Various Proline-Rich Proteins. Susumu Maruyama, Shinsuke Miyoshi, Takasumi Osa, and Hideoki Tanaka . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

"D-Sorbose Fermentation" from Galactitol by Pseudomonas sp. ST 24. Anisur Rahman Khan, Satomi Takahata, Hiroaki Okaya, Tetsuji Tsumura, and Ken Izumori ......

Production of Xylose from Xylan with Intracellular Enzyme System of Aspergillus niger 5-16. Hiromi Uchida, Isao Kusakabe, Yasuyuki Kawabata, Tetsuo Ono, and Kazuo Murakami . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Bioconversion of Gallic Acid into Pyrogallol by Immobilized Citrobacter freundii TB3. R. Ajay Kumar, Arul Jayaraman, Manickam Lakshmanan, and Paramasamy Gunasekaran . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Production of Insecticidal Protein of Bacillus thuringiensis by Cultivation of Recom- binant Escherichia coll. Masatoshi Shimizu, Shinji Iijima, and Takeshi Kobayashi . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Repeated-Batch Fermentation Process Using a Thermotolerant Flocculating Yeast Con- strutted by Protoplast Fusion. Kenji Kida, Kiyotaka Kume, Shigeru Morimura, and Yorikazu Sonoda . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Removal Characteristics of Dimethyl Sulfide by a Mixture of Hyphomicrobium sp. 155 and Pseudomonas acidovorans DMR-11. Liang Zhang, Mitsuyo Hirai, and Makoto Shoda ... . . . . . . . . . . . . " . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Mineralization and Humification Pathways in Two Composting Processes Applied to Cotton Wastes. Maria Teresa Baca, Flavio Fornasier, and Maria de Nobili .........

Cloning and Characterization of SKT5, a Saccharomyces cerevisiae Gene That Affects Pro- toplast Regeneration and Resistance to Killer Toxin of Kluyveromyces lactis. Shinichi Kawamoto, Masahiro Nomura, and Takeshi Ohno . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Role of Cytochrome c-553(CO), the Second Subunit of Alcohol Dehydrogenase, in the Azide-Insensitive Respiratory Chain and in Oxidative Fermentation of Gluconobacter Species. Yasuhiko Takeda, Toshio Shimizu, Kazunobu Matsushita, Osao Adachi, and Minoru Ameyama . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

New Inducing Factors for Virginiamycin Production from Streptomyces antibioticus. Wang Li, Takuya Nihira, Shohei Sakuda, Takuo Nishida, and Yasuhiro Yamada...

Subterminal Hydroxylation of Ketoaikanes by Fungi. Takashi Yagi, Takushi Hatano, Fumio Fukui, and Sakuzo Fukui . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Stimulation of Dimeric Alkaloid Production by Near-Ultraviolet Light in Multiple Shoot Cultures of Catharanthus roseus. Kazumasa Hirata, Masato Horiuchi, Makiko Asada, Teru Ando, Kazuhisa Miyamoto, and Yoshiharu Miura . . . . . . . . . . . . . . . . . . . . . . . .

Affinity Purification of Antibodies Using Immunomicrospheres. Akihiko Kondo, Ryo Yamasaki, and Ko Higashitani . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Isolation of a New Surfactin Producer Bacillus pumilus A-l, and Cloning and Nucleotide Sequence of the Regulator Gene, psf-1. Masaaki Morikawa, Mikito Ito, and Tadayuki Imanaka . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Isolation and Characterization of Oxygen Sensitive Mutants of Escherichia coli. Yoshinobu Matsumura and Tadayuki Imanaka . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Alcaligenes hydrogenophilus Lithoautotrophy Mutants Defective in Hydrogenase(s) En- coded on Plasmid and oS4-Like Factor Encoded on Chromosome. Fusako Umeda,

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VOL. 74, 1992 CONTENTS iii

Seiko Inagaki, Hiroe Nishie, Kiyohito Yagi, and Yoshiharu Miura . . . . . . . . . . . . . . . . . . . . . Mycolytic Activity of a Micrococcus sp. Samir M. Badr-Eldin, Ossama M. EI-Tayeb,

Salwa A. Rasmy, and Hanaa A. EI-Shafei . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Comparison of Direct Glucose Oxidation by Gluconobacter oxydans subsp, suboxydans

and Aspergillus niger in a Pilot Scale Airlift Reactor. Michael Tr/iger, Gulam N. Qazi, Rainer Buse, and Ulfert Onken . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Influence of Constant and Oscillating Dissolved Oxygen Concentration on Aspergillus niger. Michael Tr~iger, Gulam N. Qazi, Rainer Buse, and Ulfert Onken . . . . . . . . . . . .

Accumulation of Polyphosphate and Substrate Gas Utilization Efficiency in PHB Accumu- lation Phase of Autotrophic Batch Culture of Alcaligenes eutrophus ATCC17697 T. Kenji Tanaka, Ayaaki Ishizaki, and Peter F. Stanbury . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Product ion of Sanguinarine by Suspension Culture of Papaver somniferum in Bioreac- tors. Jong M. Park, Sung Y. Yoon, Kenneth L. Giles, David D. Songstad, Del Eppstein, Debra Novakovski, Laurie Friesen, and Inge Roewer . . . . . . . . . . . . . . . . . . . . . . . . . . .

Degradation of Phenols by Thermophilic and Halophilic Bacteria Isolated from a Marine Brine Sample. Hideshi Yanase, Kazuyoshi Zuzan, Keiko Kita, Satoshi Sogabe, and Nobuo Kato . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Hydrolysis of Isoflavones in Soybean Cooked Syrup by Lactobacillus casei subsp, rhamno- sus IFO 3425. Shigeki Matsuda, Tadashi Miyazaki, Yunosuke Matsumoto, Riichiro Ohba, Yuji Teramoto, Naokazu Ohta, and Seinosuke Ueda . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Characterization of the Behavior of Penicillium roquefortii in Solid State Cultivation on Support by Material Balances. Christian Larroche and Jean-Bernard Gros .........

A Simple Method of Fuzzy Modeling for a Microorganism Reaction. Tetsuya Jitsufuchi, Hidenori Ishikawa, Hideo Tanaka, and Kozo Matsushima . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Genes in PHT Plasmid Encoding the Initial Degradation Pathway of Phthalate in Pseu- domonas putida. Yasutoshi Nomura, Mariko Nakagawa, Nobuo Ogawa, Satoshi Harashima, and Yasuji Oshima . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Cloning and Sequencing of the Gene Encoding Cyclodextrin Glucanotransferase from Bacillus sp. KC201. Noriyuki Kitamoto, Tetsuya Kimura, Yukio Kito, and Kunio Ohmiya . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Isolation and Nucleotide Sequence of Hansenula anomala URA3 Gene Encoding Oroti- dine-5'-Phosphate Decarboxylase. Tomoo Ogata, Yuzuru Iimura, Takaji Obata, and Gakuzou Tamura . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Transformation of Lactobacillus acidophilus TK8912 by Electroporation with pULA105E Plasmid. Kazuo Kanatani, Kazushi Yoshida, Takatsugu Tahara, Ken-ichiro Yamada, Hirosumi Miura, Masaru Sakamoto, and Masao Oshimura . . . . . . . . . . . . . . . . . .

Highly Efficient Sporulation Induced by Glutathione or Glutathione Thiol Esters in Sake 0Kyokai No. 7) and a Wild-Type Yeast. Akitsugu Kawado, Tetsuyoshi Suizu, Satoshi Imayasu, Toshiko Shigematsu, Akira Kimura, and Kousaku Murata ... . . . . . .

Localization of Candida tropicalis Peroxisomai Isocitrate Lyase Highly Expressed in Peroxisome-Proliferated Saccharomyces cerevisiae. Yasushi Kamada, Mitsuyoshi Ueda, Haruyuki Atomi, Keinosuke Oda, Tatsuo Kurihara, Nobuko Naito, Rie Ukita, Naomi Kamasawa, Masako Osumi, and Atsuo Tanaka . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Effect of Dissolved Oxygen Concentration on Monoclonal Antibody Product ion in Hybri- doma Cell Cultures. Tatsuya Ogawa, Masamichi Kamihira, Hitoshi Yoshida, Shinji Iijima, and Takeshi Kobayashi . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Product ion of L( + )-Lactic Acid by Immobilized Cells of Rhizopus oryzae with Polymer Supports Prepared by T Ray Induced Polymerization. Masao Tamada, Anjuman Ara Begum, and Suharni Sadi . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Ethanol Production by Zymomonas mobilis Using Natural Rubber Waste as a Nutritional Source. Sudarut Tripetchkul, Michio Tonokawa, and Ayaaki Ishizaki . . . . . . . . . . . . . . .

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NOTES

Occurrence of a NADH-dependent Methylglyoxal Reducing System: Conversion of Methylglyoxal to Acetol by Aldehyde Reductase from Hansenula mrakii. Yoshiharu Inoue, Shigeaki Ikemoto, Koji Kitamura, and Akira Kimura . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Natural Distribution of Bacteria Metabolizing Many Kinds of Polycyclic Aromatic Hydro- carbons. Hohzoh Kiyohara, Noboru Takizawa, and Kazutaka Nagao . . . . . . . . . . . . . . .

Pure Isolation of a New Chemoautotrophic Ammonia-Oxidizing Bacterium on Gellan Gum Plate. Reiji Takahashi, Naomi Kondo, Kazuhito Usui, Tsutomu Kanehira Masayuki Shinohara, and Tatsuaki Tokuyama . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Anaerobic Degradation of 5-Hydroxypentanoate by a Newly Isolated Clostridium sp. Kazuhiro Tanaka . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

The Tetracycline Inducible Expression of a-Amylase in Bacillus subtilis. Takayuki Ikeda, Hisato Yamazaki, Kunihiko Yamashita, and Ryu Shinke . . . . . . . . . . . . . . . . . . . . . . . .

Effects of Light Intensity, Light Quality, and Illumination Cycle on Astaxanthin Forma- tion in a Green Alga, Haematococcus pluvialis. Makio Kobayashi, Toshihide Kakizono, Naomichi Nishio, and Shiro Nagai . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Product ion of Streptomycin Using a Computer-Controlled Fermentor: Addit ion of Glu- cose with Oxygen Uptake Rate and /o r Carbon Dioxide Evolution Rate Monitoring. Norio Shimizu, Yooji Odawara, and Kenji Katoo . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Feedback-Insensitive Mutation of 3.Deoxy-D-Arabino-Hepturosonate-7-Phosphate Syn- thase Caused by a Single Nucleotide Substitution of A R 0 4 Structural Gene in Sac- charomyces cerevisiae. Kazuro Fukuda, Kozo Asano, Kozo Ouchi, and Seigo Takasawa . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Effect of Polyvinyl Alcohol on Kinetic and Thermal Properties of ~-Galactosidase. Osamu Ariga, Tetsuya Sano, and Yoshiki Sano . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Behaviour of Grape Must Ferment Saccharomyces cerevisiae Immobilized within Insolubi- lized Gelatin. Palma Parascandola, Elisabetta de Alteriis, Giovanni Antonio Farris, Marilena Budroni, and Vincenzo Scardi . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Cellulase Product ion Profile of Trichoderma reesei on Different Cellulosic Substrates at Various pH Levels. Parminder S. Chahal, Devinder S. Chahal, and G6rard Andr6...

The Influence of Shear Stress and Its Reduction in the Product ion of Saccharifying Enzyme from Shochu Distillery Wastewater by Aspergillus awamori var. kawachL Shigeru Morimura, Kenji Kida, and Yorikazu Sonoda . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Product ion and Characteristics of Sprouting Rice Wine. Seinosuke Ueda, Yuji Tera- moto, Riichiro Ohba, and Shoji Kayashima . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Analysis of Plasmid Stability by the Difference in Colony Size. Sunghoon Park and Dewey D. Y. Ryu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Synthesis of L-ProUne from the Racemate by Coupling of Enzymatic Enantiospecific Oxi- dation and Chemical Non-Enantiospecific Reduction. Jeong Weon Huh, Kumio Yokoigawa, Nobuyoshi Esaki, and Kenji Soda . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

A New Bile Acid Glycoside Produced by Cunninghamella blakesleeana ST-22 from Lithocholic Acid. Takuya Nihira, Yusaku Okada, and Yasuhiro Yamada .. . . . . . . .

Inhibition of Hiochi-Bacteria by Nisin. Kazuo Kanatani, Takatsugu Tahara, Kazushi Yoshida, Ken-ichiro Yamada, Hirosumi Miura, Masaru Sakamoto, and Masao Oshimura . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Glucose-Limited Fed-Batch Culture of Escherichia coil for Product ion of Recombinant Human Interleukin-2 with the DO-Stat Method. Seo, Dong Jin, Bong Hyun Chung, Young Bo Hwang, and Young Hoon Park . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Cloning and Nucleotide Sequence of the Regulatory Gene, degM, for Minor Serine Pro- tease in Bacillus subtilis. Akihiko Masui, Nobuaki Fujiwara, Masahiro Takagi, and

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Tadayuki Imanaka . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Revised Sequence and Activity of Bacillus stearothermophilus Catalase I (Formerly Peroxi-

dase). Savitr Trakulnaleamsai, Satoshi Aihara, Kouji Miyai, Yasuichiro Suga, Mikiko Sota, Tetsuya Yomo, and Itaru Urabe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Characterization and Mode of Action of Exo-l,3-fl-D-Glucanase from Aspergillus saitoL Shin Kasahara, Tasuku Nakajima, Chikara Miyamoto, Kenji Wada, Yasuhiro Furuichi, and Eiji Ichishima . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Rapid Transformation of Bacillus subtilis Using KCI-Treatment. Hideji Hiraoka, Takashi Ano, and Makoto Shoda . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Isolation and Characterization of a Fast-Growing, Thermophilic, Hydrogenotrophic Methanogen. Shinko Kitaura, Norihiro Nishimura, Akio Mimura, and Yoshimasa Takahara . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Characterization of Newly Isolated Bacillus subtilis C1 Which Is Sensitive to Antibiotic Produced by Lactic Acid Coccus. Ayaaki Ishizaki, Motoi Tamura, Katsunori Kimura, Yojiro Koba, Michio Tonokawa, and Takayuki Ezaki . . . . . . . . . . . . . . . . . . . . . . . . . . .

Secretion of the Glucoamylase-fl-Lactamase Chimera Protein by Saccharomyces cerevisiae. Dai Hirata, Tokichi Miyakawa, and Ichiro Yamashita . . . . . . . . . . . . . . . . . . . . .

In Vivo Cloning of a Pseudomonas oxalaticus o~4-Like Gene Acting in Expression of Hydrogenase Genes from Alcaligenes hydrogenophilus. Fusako Umeda, Yoko Tokuyama, Tomoko Seko, Kiyohito Yagi, and Yoshiharu Miura . . . . . . . . . . . . . . . . . . . . . . . .

Molecular Cloning of a Gene Responsible for the Biosynthesis of the Lipopeptide Antibi- otics Iturin and Surfactin. Hideji Hiraoka, Takashi Ano, and Makoto Shoda ......

Opt imum Culture Conditions for Production of Kefiran by Lactobacillus sp. KPB-167B Isolated from Kefir Grains. Haruhiko Yokoi and Takashi Watanabe . . . . . . . . . . . . . . . . . .

Recovery of Intact Membrane Proteins from Adherent Animal Cells Grown in a Liquid- Liquid Interface. Shoichi Terada, Masayuki Sato, Rie Katayama, and Takao Shinozawa . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Transformation of the Industrial Strain of Aspergillus oryzae with the Homologous amdS Gene as a Dominant Selectable Marker. Katsuya Gomi, Katsuhiko Kitamoto, and Chieko Kumagai . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Comparison of Xylan and Methyl fl-Xyloside-Induced Xylanases from Streptomyces sp. Kotoyoshi Nakanishi, Masaki Marui, and Tsuneo Yasui . . . . . . . . . . . . . . . . . . . . . . . . . . .

Microbial Reduction of Cyclohexanone by Chlorella pyrenoidosa Chick. Fumiki Yoshizako, Atsuo Nishimura, and Mitsuo Chubachi . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Sustained Production of Arachidonic and Eicosapentaenoic Acids by the Red Alga Porphyridium purpureum Cultured in a Light /Dark Cycle. Souichi Ohta, Thomas Chang, Osamu Aozasa, Masaomi Kondo, and Hideaki Miyata . . . . . . . . . . . . . . . . . . . . . . . . . . .

Effect of Carbon/Nitrogen Ratio on Encystment Accompanied with Astaxanthin For- mation in a Green Alga, Haematococcus pluvialis. Toshihide Kakizono, Makio Kobayashi, and Shiro Nagai . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Influence of Carbon Sources on the Stimulatory Effect of L-Proline on 5'-Inosinic Acid Production. Kazuhiro Tomita and Yoshiyuki Kuratsu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Factors Affecting the Activity of a Protein Bioflocculant Produced by Nocardia amarae. Minoru Takeda, Jun-ichi Koizumi, Hiroshi Matsuoka, and Motohiko Hikuma ......

A Novel Method of Immobilizing Penicillin Acylase on Basic Anion Exchange Resin with I-Iexamethylene Diisocyanate and p-Hydroxybenzaldehyde. Kobee Kobayashi, Bunji Kageyama, Shigeo Yagi, and Takayasu Sonoyama . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Sewage Treatment under Conditions of Balancing Microbial Growth and Cell Decay with a High Concentration of Activated Sludge Supplemented with Ferromagnetic Pow- der. Yasuzo Sakai, Kazuaki Tani, and Fujio Takahashi . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

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