Journal of Fermentation and Bioengineering

CONTENTS

VOLUME 79 l 1995

ORIGINALS

Development of an Integrative DNA Transformation System for the Yeast Hansen- ula anomala. Tomoo Ogata, Yasushi Okumura, Yuzuru Iimura, and Takaji Obata . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Isolation and Characterization of Zygosaccharomyces rouxii Mutants Defective in Proton Pumpout Activity and Salt Tolerance. Shigetoshi Yoshikawa, Ken-ichi Chikara, Hajime Hashimoto, Nobuo Mitsui, Makoto Shimosaka, and Mitsuo Okazaki . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ..f..............

Reconstruction of Ethanol Fermentation in Permeabilized Cells of the Yeast Saccharomyces cerevisiae. Kazuhiko Takeshige and Kozo Ouchi . . . . . . . . . . . .

Purification and Some Properties of Aspergillus pulverulentus BXylosidase with Transxylosylation Capacity. Joko Sulistyo, Yoshi Kamiyama, and Tsuneo Yasui . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

High-Level Production of Recombinant Glutamic Acid-Specific Protease from Bacilllus licheniformis in Bacillus subtilis Expression System. Koichi Matsumoto, Kenji Mitsushima, Mikio Tamaki, Hideo Iwamoto, Shinji Kakudo, Hiroyuki Okamoto, Hiroshige Tsuzuki and Hiroshi Teraoka . . . . .

Microbial Asymmetric Reduction of (RS)-2-(4-Methoxyphenyl)-1,5-benzothia- zepin-3,4(2H,SH)-dione, the Key Intermediate in the Synthesis of Diltiazem Hydrochloride. Hiroaki Matsumae, Hiroshi Douno, Shinichi Yamada, Takuo Nishida, Yasuhiko Ozaki, Takeji Shibatani, and Tetsuya Tosa . . . . . .

Regioselective Bioconversion of Colchicine and Thiocolchicine into Their Corre- sponding 3-Demethyl Derivatives. Alexander Poulev, Ezio Bombardelli, Cesare Ponzone, and Meinhart H. Zenk . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Characterization of New Denitrifying Rhodobacter Strains Isolated from Photosynthetic Sludge for Wastewater Treatment. Akira Hiraishi, Keigo Muramatsu, and Katsuro Urata . . . . . . . . . . . . . . . . . . . . . . . . . .

Modeling of the Sensory Evaluation of Sake by Dempster-Shafer’s Measure and Genetic Algorithm. Kazuo Matsuura, Masato Hirotsune, Masaaki Hamachi, and Yataro Nunokawa ..,.....................,.............................

Use of Chemostat Data for Modelling Extracellular-Inulinase Production by Kluyveromyces marxianus in a High-Cell-Density Fed-batch Process. Marco C. M. Hensing, Johannes S. Vrouwenvelder, Chris Hellinga, Johannes P. van Dijken, and Jack T. Pronk . . . . . . . . . . . . . .., . . . . . . . . . . . . . . . . . .

Gene Cloning and Characterization of Thermostable Peptidyl Prolyl cis-trans Isomerase (PPIase) from Bacillus stearothermophilus SICl. Dong-Ju Kim, Masaaki Morikawa, Masahiro Takagi, and Tadayuki Imanaka . . . . . . . . . . . . . . .

Development of a Stable Shuttle Vector and a Conjugative Transfer System for Gluconobacter oxydans. Masako Shinjoh and Tatsuo Hoshino . . . . . . . . . . . . .

Trichloroethylene Degradation by Genetically Engineered Bacteria Carrying Cloned Phenol Catabolic Genes. Masanori Fujita, Michihiko Ike, Jun-ichi Hioki, Koji Kataoka, and Masahiro Takeo . . . . . . . . . . . . . . . . . . . . . . . . .

Experimental Sketch of Landscapes in Protein Sequence Space. Savitr

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Trakulnaleamsai, Tetsuya Yomo, Masako Yoshikawa, Satoshi Aihara, and Itaru IJrabe . . . . . . . . . .

Identification of Cholesterol Oxidase from Fast-Growing Mycohacterial, Strains and Rhodococcus sp. Danuta Wilmanska, Jaroslaw Dziadek, Anna Sajduda, Krystyna Milczarek, Adam Jaworski, and Yoshikatsu Murooka

Purification and Characterization of a Protease-Resistant Cellulase from Asper- gillus niger. Shunichi Akiba, Yoshiharu Kimura, Kenji Yamamoto, and Hidehiko Kumagai . . . .

Cell-Free System Derived from Heat-Shocked Escherichiu coli: Synthesis of Enzyme Protein Possessing Higher Specific Activity. Norihiro Nishimura, Yoshihisa Kitaoka, and Mitsuru Niwano . . . . .

Toxic Effects of Hg2+ to a Hg *+-Resistant Pseudomonas in the Presence of Halide Ions. Ma. Lourdes B. Buelva, Kazuo Kakii, and Mitsuo Kuriyama

Biotransformation of Cyclic /3-Keto Esters by ChZoreZZa pyrenoidosa Chick. Fumiki Yoshizako, Masayo Ogino, Atsuo Nishimura, Mitsuo Chubachi, and Toyokazu Horii . . . . . . _. _. . .

Sophorolipids Production by Cundida bombicola Using Self-Cycling Fermenta- tion. William C. McCaffrey and David G. Cooper . . . .

Effect of Cell Movement by Random Mixing between the Surface and Bottom of Photobioreactors on Algal Productivity. James C. Ogbonna, Hirokazu Yada, and Hideo Tanaka . . . . . . .

Continuous Culture Study of Transient Behaviour of Succhuromyces cerevisiae Growing on Sucrose and Fructose. John P. Barford, Joanna H. Johnston, and Patrick K. Mwesigye . . . . .

Observation of Microorganism Colonies Using a Scanning-Laser-Beam pH-Sens- ing Microscope. Motoi Nakao, Shinji Inoue, Ryu Oishi, Tatsuo Yoshinobu, and Hiroshi Iwasaki . . . . . . . . . . . .

Pseudo-Steady State Oxygen-Concentration Profiles in an Agar Slab Containing Growing Nitrobacter agilis. RenC H. Wijffels, Meinard R. Eekhof, Dirk de Beer, Johannes C. van den Heuvel, and Johannes Tramper . . . . .

Characterization In Vitro of the Hydroxylase Component of Xylene Monooxy- genase, the First Enzyme of the TOL-Plasmid-Encoded Pathway for the Mineralization of Toluene and Xylenes. Jeffrey P. Shaw and Shigeaki Harayama . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Isolation and Characterization of Pz-Peptidase from Bacillus Zicheniformis N22. Somsit Asdornnithee, Eiichi Himeji, Kouichi Akiyama, Takeshi Sasaki, and Renkichi Takata . . . . . .

Overexpression and In Vitro Reconstitution of the Ethylene-Forming Enzyme from Pseudomonas syringae. Katsuya Ishihara, Masayoshi Matsuoka, Yorinao Inoue, Sumio Tanase, Takahira Ogawa, and Hideo Fukuda . .

Purification and Properties of Cellobiose Phosphorylase from Clostridium ther- mocellum. Keijitsu Tanaka, Takashi Kawaguchi, Yukio Imada, Toshihiko Ooi, and Motoo Arai . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Purification, Characterization and Structure Analysis of NADPH-Dependent D-

Xylose Reductases from Candida tropicalis. Shin-ichiro Yokoyama, Tohru Suzuki, Keiichi Kawai, Hiroyuki Horitsu, and Kazuhiro Takamizawa

Kinetics and Mechanism of Acyl Transfer by Penicillin G Acylase Comparing Acyl Donors Methyl Chloroacetate and Methyl Phenylacetate. Kouji Isaka, Naoko Suga, and Fumihiro Ishimura.. . . . .

Daunomycin Biosynthesis by Microbial Conversion of Precursor Metabolites Using Biosynthetically Blocked Mutants. Akihiro Yoshimoto, Tomohiko

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Nakamura, Katsuro Kubo, Osamu Johdo, and Hiroshi Tone . . . . . . . . . . . . . . .

Maximum Yield of Foreign Lipase in Escherichiu coli HBlOl Limited by Dura- tion of Protein Expression. Dae Hyun Hahm, Soo Hyoung Kim, Jaegu Pan, and Joon Shick Rhee.. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Production of Fructosylxylosides by Scopulariopsis brevicaulis sp. Hiroyuki Takeda and Shinichi Kinoshita . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Non-Fragmenting Variants of Streptomyces Hyphae Have Enhanced Activity of an Enzyme (Phospho-N-Acetylmuramyl Pentapeptide Translocase) in Peptidoglycan Biosynthesis. Karen M. Pickup and Michael E. Bushel1 .

Growth of Submerged Mycelia of AspergilZus kawachii in Solid-State Culture. Shigetoshi Sudo, Seiji Kobayashi, Akihiro Kaneko, Kazuo Sato, and Toshiteru Oba.. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Light Emitting Diode-Based Algal Photobioreactor with External Gas Ex- change. Choul-Gyun Lee and Bernhard 0. Palsson . . . . . . . . . .

On-Line Monitoring and Control of Acetone-Butanol Fermentation by Mem- brane-sensor Mass Spectrometry. Somchai Chauvatcharin, Tatsuji Seki, Kazuhito Fujiyama, and Toshiomi Yoshida . . . . . , . . . . . . . . . . . . . .

Improved Performance of a Hybrid Design Over an Anaerobic Filter for the Treatment of Dairy Industry Wastewater at Laboratory Scale. Pedro R. Cordoba, Alejandro P. Francese, and Faustino Siiieriz . . . . . . . . . . . . . . . . . . . . . .

Comparative Composition and Characteristics of Methanogenic Granular Sludges Treating Industrial Wastes under Different Conditions. Zafar Iqbal Bhatti, Kenji Furukawa, and Masanori Fujita . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Enhanced Production of Peroxidase in Continuous Culture of Arthromyces ramosus by Selective Bleeding of Mycehum. Pei-Xin Yao, Nobuya Matsumoto, Hideo Tsujimura, Masamitsu Takaya, and Kiyoshi Toda . . . . . . . . . . . . . .

Transphosphatidylation Reaction of Phosphatidylcholine to 4-Methoxyphenol in Water-Immiscible Organic Solvents with Immobilized Phospholipase D. Masaaki Takami and Yukio Suzuki . . . . . . . . . . . . . . . . . . . . . . . . . . .

Continuous Production of L-Aspartic Acid from Ammonium Fumarate Using Immobilized Cells by Capture on the Surface of Nonwoven Cloth Coated with a Pyridinium-Type Polymer. Nariyoshi Kawabata, Hidetaka Hatanaka, and Hidetoshi Odaka . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Conversion of u-Psicose to Allitol by Enterobacter ugglomeruns Strain 221e. Syed Muniruzzaman, Hisahiro Tokunaga, and Ken Izumori.. . . . . . . . .

Biosynthesis of Poly(3-Hydroxybutyrate-co-3-Hydroxyvalerate) by Newly Isolat- ed Agrobacterium sp. SH-1 and GW-014 from Structurally Unrelated Single Carbon Substrates. Eun Yeol Lee, Sang Hyeon Kang, and Cha Yong Choi . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Optimal Disruption Methods for the Selective Recovery of BGalactosidase from Escherichia coli. Ryoichi Kuboi, Hiroshi Umakoshi, Naoyuki Takagi, and Isao Komasawa . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . _. . . . . . . . . _. . . . . . . . . . _

Strategy for Inducing Pertinent Cell Line and Optimization of the Medium for Stizolobium hassjoo Producing L-DOPA. Shih-Yow Huang, Shi-Ying Chen, Kuo-Long Wu, and Wei-Tai Taung . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Characteristics of Granular Methanogenic Sludge Grown on Phenol Synthetic Medium and Methanogenic Fermentation of Phenolic Wastewater in a UASB Reactor. Yong-Jin Chang, Naomichi Nishio, and Shiro Nagai . . . . . . . . . . . . .

High Rate Performance and Characterization of Granular Methanogenic Sludges in Upflow Anaerobic Sludge Blanket Reactors Fed with Various Defined Substrates. Satoshi Fukuzaki, Naomichi Nishio, and Shiro Nagai. . . . . .

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Expression of Recombinant Antibody against Cancer-Specific Carbohydrate. Kuniaki Takagi, Kiyoshi Yasukawa, Yoshitaka Iba, Yuji Isobe, Yasunobu Suketa, Reiji Kannagi, Katsunori Kohda, Masahiro Takagi, and Tadayuki Imanaka . . . . . . . . . . . . . . . . . . . . . . . . .

Genetic Modification of the Streptomyces Cholesterol Oxidase Gene for Expres- sion in Escherichia coli and Development of Promoter-Probe Vectors for Use in Enteric Bacteria. Nobuhiko Nomura, Kwang-Pi1 Choi, Mitsuo Yamashita, Hiroko Yamamoto, and Yoshikatsu Murooka . . .

Extracellular Production of Phospholipase D of Streptomyces antibioticus Using Recombinant Escherichia coli. Yugo Iwasaki, Naoto Mishima, Kousaku Mizumoto, Hideo Nakano, and Tsuneo Yamane.. . . . .

Cloning of the xynNS Gene Encoding Xylanase B from Aspergillus niger and Its Expression in Aspergillus kawachii. Kotaro Kinoshita, Makoto Takano, Takuya Koseki, Kiyoshi Ito, and Kimio Iwano . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Identification of Malting Barley Varieties by Genome Analysis. Youichi Tsuchiya, Shigeki Araki, Hirohisa Sahara, Masachika Takashio, and Shohei Koshino . . . . . . . . . . .

Thermostable Alkaline Lipase from a Newly Isolated Thermophilic Bacillus, Strain A30-1 (ATCC 53841). Yongxiang Wang, Kailash C. Srivastava, Gwo-Jenn Shen, and Henry Y. Wang . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

R-( - )-Mandelic Acid Production from Racemic Mandelic Acids by Pseudomonas polycolor with Asymmetric Degrading Activity. Eiji Takahashi, Katsuhiko Nakamichi, Masakatsu Furui, and Takao Mori . . . .

Comparison of Neomycin Production from Streptomyces fradiae Cultivation Using Soybean Oil as the Sole Carbon Source in an Air-Lift Bioreactor and a Stirred-Tank Reactor. Noriyasu Ohta, Yong Soo Park, Kazutoyo Yahiro, and Mitsuyasu Okabe . . . . . . . . . . . . . . . _. . . . . . . .

Factors Affecting the Ethanol Productivity of Yeast in Molasses. Kazuhiko Takeshige and Kozo Ouchi . . _. . . _. . . . . . . . . . . . . . . . . . _. . . . . . . _ _. . . .

Promotive Effect of 5-Aminolevulinic Acid on the Growth and Photosynthesis of Spirulina platensis. Ken Sasaki, Facundo J. Marquez, Naomichi Nishio, and Shiro Nagai . . . . . . . . . . . . . . . . . . . . . . .

Production of Plantlets for Use as Artificial Seeds from Horseradish Hairy Roots Fragmented in a Blender. Yutaka Nakashimada, Nobuyuki Uozumi, and Takeshi Kobayashi _. . . . . . . . . . . . . .

A Mass Spectrometry Membrane Probe and Practical Problems Associated with Its Application in Fermentation Processes. Somchai Chauvatcharin, Konstantin B. Konstantinov, Kazuhito Fujiyama, Tatsuji Seki, and Toshiomi Yoshida . . . . . . . . . . . . . . . . . . .

Effect of Water Activity on Enzyme Hydration and Enzyme Reaction Rate in Organic Solvents. Sun Bok Lee and Ki-Jo0 Kim . . . . . . . .

Enzyme Reaction Kinetics in Organic Solvents: A Theoretical Kinetic Model and Comparison with Experimental Observations. Sun Bok Lee . .

Polymerase Chain Reaction Amplification and Restriction Fragment Length Poly- morphism Analysis of 16s rRNA Genes from Methanogens. Akira Hiraishi, Yoichi Kamagata, and Kazunori Nakamura . . . . . . . . . . . .

Expression of a celE Gene from Clostridium thermocellum in Bacillus. Rustem I. Aminov, Nikolai P. Golovchenko, and Kunio Ohmiya . . . . . .

Direct Uptake of Alginate Molecules through a Pit on the Bacterial Cell Surface: A Novel Mechanism for the Uptake of Macromolecules. Tomohiro Hisano,

Yoshimasa Yonemoto, Tetsuo Yamashita, Yasuki Fukuda, Akira Kimura,

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and Kousaku Murata . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Immunoadsorption of Impurities Contained in Recombinant Human Serum

Albumin Secreted from Yeast. Shigeo Katoh and Kazuya Watanabe . . . . . .

Production and Characteristics of Some New &Agarases from a Marine Bacteri- um, Vibrio sp. Strain JT0107. Yasushi Sugano, Hideki Nagae, Koji Inagaki, Takeshi Yamamoto, Ichiro Terada, and Yoshinari Yamazaki . . . . . . . . . . . . . . . . . .

Control of Intracellular Ammonium Level Using Specific Oxygen Uptake Rate for Overproduction of Citric Acid by Aspergillus niger. Kong Sik Kim, Young Je Yoo, and Min Hong Kim . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Breeding of High Glycerol-Producing Shochu Yeast (Succharomyces cerevisiue) with Acquired Salt Tolerance. Toshiro Omori, Kiyoshi Ogawa, and Masahiko Shimoda . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Influence of the Concentrations of o-Xylose and Yeast Extract on Ethanol Produc- tion by Pachysolen tannophilus. Vicente Bravo, Fernando Camacho, Sebastian Sanchez, and Eulogio Castro . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Effects of Agitator Configuration and Rotational Speed on the Production of Extracellular Polysaccharide by Xunthomonas cucurbitae PCSIR B-52. Shahjahan Baig and Hafiz Sameer Ahmad . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Enhancement of tPA Production under Hyperoxic Conditions by BHK Cells in Serum-Free and Serum-Containing Cultures. Jin Wang, Hiroyuki Honda, Hideki Watanabe, and Takeshi Kobayashi . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Efficient Production of Celery Embryos and Plantlets Released in Culture of Immobilized Gel Beads. Ken-ichiro Suehara, Katsuhito Kohketsu, Nobuyuki Uozumi, and Takeshi Kobayashi.. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Effective Lactic Acid Production by Two-Stage Extractive Fermentation. Hiroyuki Honda, Yoshio Toyama, Hiroshi Takahashi, Takuo Nakazeko, and Takeshi Kobayashi . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Extractive Fermentation of Gibberellic Acid by Gibberella fujikuroi. Dirk Hollmann, Jorn Switalski, Sven Geipel, and Ulfert Onken . . . . . . . . . . . . . . . . . . . . .

Characteristics of a Newly Isolated Fungus, Geotrichum candidurn Dee 1, Which Decolorizes Various Dyes. Seong Jun Kim, Kenichi Ishikawa, Mitsuyo Hirai, and Makoto Shoda . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Trichloroethylene Degradation by Cells of a Methane-Utilizing Bacterium, Methylocystis sp. M, Immobilized in Calcium Alginate. Hiroo Uchiyama, Kazuhito Oguri, Mari Nishibayashi, Etsuo Kokufuta, and Osami Yagi . . . . . .

NOTES

In Mvo Recombination of Two R68.45-Primes, One Carrying Plasmid Genes for Autotrophy (Aut) from Alcaligenes hydrogenophilus and the Other Carrying a d4-Like Chromosome Gene from Pseudomonas oxalaticus. Fusako Umeda, Tomoko Seko, Kazuhiro Yano, Noritsugu Goda, Kiyohito Yagi, Yoshiharu Miura, and Tadashi Mizoguchi . . . . . . . . . . . . . ..f..........................

Determination of the Transcriptional Initiation Sites of the Kanamycin Resistance Genes of pUBll0 and pTHN1. Takayuki Hoshino, Shima Eda, Yukihiko Fukase, and Masanao Oda . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Effects of Elevated Expression of the CYPSI (P45014DM) Gene on the Sterol Con- tents of Saccharomyces cerevisiae. Hitoshi Tainaka, Hideaki Hashimoto, Yuri Aoyama, and yuzo Yoshida . . . . . . . . . . . . . . . . . . . . . . . . , . . . . . . . . . . . . . . . . . .

Purification and Properties of a Citrate Synthase from Nitrosomonus sp. TK794 and a Comparison with the Enzymes of Another Nitrifying Bacteria.

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Tomohide Asai, Reiji Takahashi, Tomoko Fujioka, and Tatsuaki Tokuyama . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ..__........._..................

Enzymatic Production of D-Glutamate from L-Glutamate by a Glutamate Racemase. Makoto Yagasaki, Masaki Azuma, Shuichi Ishino, and Akio Ozaki . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Participation of Reactive Oxygens in the Formation of a Purple Pigment, Hordeu- min, from Barley Bran-Fermented Broth. Riichiro Ohba, Ichiro Nakayama, Ikuo Suda, and Seinosuke Ueda . . . . . . . . . .

Efficient Release of Thermostable ,&Galactosidase by Glycine Addition and Ther- mal Treatment of Escherichiu coli Cells. Osamu Ariga, Takao Yoshinaga, and Yoshiki Sano . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .._...................

Repression of Mycelia Extension from a Support Carrier Containing Immobilized Growing Aspergillus oryzae. Yasuhiko Imai, Katsunori Ishikawa, Roberto D. Ayo, Masaru Suzuki, Masatoshi Matsumura, and Hiroshi Kataoka . . .

A Facile Preparation of Enantiomers of Ethyl 4,4,4-Trifluoro-3-(Indole-3-) Butyrate, a Novel Plant Growth Regulator. Katsuya Kato, Masato Katayama, Rakesh K. Gautam, Shozo Fujii, Haruhiko Fukaya, and Hiroshi Kimoto . . . . . . . . .

Properties of Zinc Uroporphyrin III Produced from Isopropanol by Arthrobacter hyalinus. Masahiro Kajiwara, Shinji Tokiwa, Kazuhiko Takatori, and Ichiro Kojima . . . . . . . . . . . . . . . . . . . . .._.._............................._......__.................

Stimulatory Effects of Amino Acids and Oleic Acid on Poly(3-Hydroxybutyric Acid) Synthesis by Recombinant Escherichia coli. Sang Yup Lee, Yoo Kyung Lee, and Ho Nam Chang.........................................................

L-Serine Synthesis Using the Resting Hyphomicrobium sp. NCIB10099 Cells under Suppressive Conditions for L-Serine-Degrading Activity. Toyokazu Yoshida, Tairo Hagishita, Toshio Mitsunaga, and Yoshikazu Izumi .

Preparation of D-Sorbose from D-Tagatose by Immobilized D-Tagatose 3- Epimerase. Hiromichi Itoh, Tomoko Sato, Tomohiro Takeuchi, Anisur Rahman Khan, and Ken Izumori . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ..I...........

Comparative Studies on Utilization of Fatty Acids and Hydrocarbons in Nocardiu amarae and Rhodococcus spp. Keisuke Iwahori, Min Wang, Hironori Taki, and Masanori Fujita . . . . . . .

Some Factors Which Affect the Phosphorus Uptake of a Phosphorus-Accumulat- ing Bacterium, Strain NM-l. Mamoru Kawaharasaki and Kazunori Nakamura . . . . . . . . . . . . . . . . . . . . . . .

Enhancement of Transgene Expression in Mammalian Cell Line by a A’- Prostaglandin A1 Analogue. Taichi Matsuda, Toshiyuki Takai, Masaki Hikida, Tatsuji Yasuda, Seizi Kurozumi, and Hitoshi Ohmori.. . .

Comparison of Liposome Immune Lysis Assay (LILA) and Enzyme-Linked Immu- nosorbent Assay (ELISA) for Detection of Antibodies. Kanji Tomioka, Hideki Fukuda, and Shigeo Katoh . . . . . . . . . . . . . . . . . . . . . . . . . . .._.....................

A Selective Isolation Method for Actinomadura viridis in Soil. Masayuki Hayakawa, Yuka Momose, Takayuki Kajiura, Toyohiko Yamazaki, Tomohiko Tamura, Kazunori Hatano, and Hideo Nonomura . . . . . . . . . . .

Growth of Moderately Thermophilic Iron-Oxidizing Bacterium Strain TI-1 in Syn- thetic Medium. Tsuyoshi Sugio, Kimiko Kishimoto, Masaki Takai, Keiichi Oda, and Tatsuo Tano . . . . . . . .

Ethanol Fermentation Promoted by Delignified Cellulosic Material. Lazaros Iconomou, Costas Psarianos, and Athanasios Koutinas.. .

Hybrid Simulation of Microbial Behavior Combining a Statistical Procedure and

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VOL. 79, 1995 CONTENTS vi

Fuzzy Identification of Culture Phases. Jun-ichi Horiuchi, Michimasa Kishimoto, and Haruo Momose . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Thermochemical Liquidization and Anaerobic Treatment of Dewatered Sewage Sludge. Shigeki Sawayama, Seiichi Inoue, Tatsuo Yagishita, Tomoko Ogi, and Shin-ya Yokoyama . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Host Range Specificity of a Novel Runaway-Type Vector for Mammalian Cells. Hideyo Kirinaka, Masamichi Kamihira, and Shinji Iijima . . . . . . . . . . . . .

Secretive Expression of the Aspergillus aculeatus Cellulase (FI-CMCase) by Saccharomyces cerevisiae. Kazuhisa Minamiguchi, Toshihiko Ooi, Takashi Kawaguchi, Hirosuke Okada, Sawao Murao, and Motoo Arai.. . . . . . .

Purification, Crystallization, and Characterization of the Extracellular Invertase from Zymomonas mobilis. Hideshi Yanase, Mayumi Iwata, Keiko Kita, Nobuo Kato, and Kenzo Tonomura . . . . . . . . . . . . . . . . . . . . . .

Purification and Properties of Mycodextranase from Bacillus circulans NHB-1. Katsuichiro Okazaki, Sheng Yong Jin, Yutaka Sawamura, and Shigeru Hayakawa . . . . . . . . . . . . .._.....................................................................

Diffusional and Electrostatic Effects on Apparent Kinetic Parameters of Reactions Catalyzed by Enzyme Immobilized on the External Surface of a Support. Fumihidc Shiraishi . . . . . . . . . . . . . . . . . . . . _. . . . _. . . . . . . .

Characteristics of a Biopolymer Flocculant Produced by Bacillus sp. PY-90. Haruhiko Yokoi, Osamu Natsuda, Jun Hirose, Sachio Hayashi, and Yoshiyuki T&as&i . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

DO-Stat Fed-Batch Production of cis,cis-Muconic Acid from Benzoic Acid by Pseudomonas putida BM014. Sang-Gu Bang and Cha Yong Choi .

Production of C,-Symmetrical Phenazines by Some Actinomycetes. Ikunosuke Tanabe, Masayuki Kuriyama, and Hideo Nonomura . . . . . . . . . . . . . . . . . . . . . . . . . . .

Substrate Permeability in Pellets Formed by Aspergillus niger. Keisuke Iwahori, Sigeru Tatsuta, Masanori Fujita, and Koichiro Yamakawa . . . . . . . . . . . . . . . . . . . . .

Effect of Dissolved Oxygen and pH on Moranoline (l-Deoxynojirimycin) Fermen- tation by Streptomyces lavendulae. Masahiko Kojima, Noriyuki Tachikake, Yohsuke Kyotani, Kiyotaka Konno, Shigeaki Maruo, Masashi Yamamoto, and Tohji Ezure . . . . . . . . . . . . . . . . . . . . . _. . . . . . . . .

Biological Production of Hydrogen from Cellulose by Natural Anaerobic Microflora. Yoshiyuki Ueno, Tatsushi Kawai, Susumu Sato, Seiji Otsuka, and Masayoshi Morimoto.. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Biomethanation of Cheese Whey Using Anaerobic Upflow Fixed Film Reactor. Priti PateI, Manik Dcsai, and Datta Madamwar . . . . . .

Inference of Methanogenic Bacteria in Wastewater Digestor Sludges by Lipid Component Analysis. Masateru Nishihara, Masayo Akagawa-Matsushita, Yoshitaka Togo, and Yosuke Koga . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .._

Molecular Cloning and Sequencing of the Phenol Hydroxylase Gene from Pseu- domonas putida BH. Masahiro Takeo, Yoshimichi Maeda, Hiroshi Okada, Kanako Miyama, Kazuhiro Mori, Michihiko Ike, and Masanori Fujita . .

Production of Recombinant Cytochrome c-551 in a Pseudomonas aeruginosa Mutant Strain. Hiroyuki Arai, Yan Zhang, Yoshihiro Sambongi, Yasuo Igarashi, and Tohru &&ma . . . . . . . . . . . . . . . . . . . . . .

Production of Ubiquinone in Escherichia coli by Expression of Various Genes Responsible for Ubiquinone Biosynthesis. Xufen Zhu, Mihoko Yuasa, Kazunori Okada, Kengo Suzuki, Tsuyoshi Nakagawa, Makoto Kawamukai, and Hideyuki Mats&a . . . _. . _. . . . . . . . . . . . . . . . . _. . . . . . . _. . . . . . . . . _. . . . . . . . . . . __.

Purification and Characterization of a Ribitol Dehydrogenase from Enterobacter

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VIII CONTENTS J. FERMENT. BIOENG

agglomerans Strain 221e. Syed Muniruzzaman, Yasutaka Kunihisa, Kenji Ichiraku, and Ken Izumori . . . . . . . . . . .

Thermostable Succinyl-Coenzyme A Synthetase from Nitrosomonas europaea ATCC 25978: Purification and Properties. Hiroshi Kondo, Takashi Ohmori, Haruka Shibata, Keiko Sase, Reiji Takahashi, and Tatsuaki Tokuyama . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .._.............................._...............

Anaerobic Degradation of 3-Hydroxypropanoate by a Newly Isolated CZostridium SP. Kazuhiro Tanaka . . . . . . . . . . . .

Breeding of Aspergillus terreus Mutant TN-484 for Itaconic Acid Production with High Yield. Kazutoyo Yahiro, Tetsushi Takahama, Yong Soo Park, and Mitsuyasu Okabe . . . . . . . . . . . . . . .

Mycelial Propagation and Enzyme Production in Koji Prepared with Aspergillus oryzae on Various Rice Extrudates and Steamed Rice. Cheng-Chun Chou and Jin-Hwei Rwan . . . . ..__................................................................

Effects of Yeast Invertase on Ethanol Production in Molasses. Kazuhiko T&eshige and Kozo Ouchi . _. _. .

Production of Monascus Pigments by a Solid-Liquid State Culture Method. Yuan-Kun Lee, Duan-Cheng Chen, Somchai Chauvatcharin, Tatsuji Seki, and Toshiomi Yoshida _. . . . . . .

Diauxie in Immobilized Microorganisms. Osamu Ariga, Masatoshi Saito, and Yoshiki Sano . _. _, _. _. _. . _. . . .

Mutants of n-Aminopeptidase with Increased Thermal Stability. Yasuhisa Asano and Kaori Yamaguchi . _. _. . _. . . . .

5-Azacytidine Enhances Transient Expression of a Transfected Gene in Cultured Mammalian Cells. Toshiyuki Takai, Tatsuji Yasuda, and Hitoshi Ohmori..

Production of L-Tagatose from Galactitol by KZebsieZZa pneumoniae Strain 40b. Tsuyoshi Shimonishi, Yasuhito Okumura, and Ken Izumori.. .

TECHNICAL NOTES

Preparation of Artificial Seeds Using Cell Aggregates from Horseradish Hairy Roots Encapsulated in Alginate Gel with Paraffin Coat. Vez Pagara Repunte, Masahito Taya, and Setsuji Tone . . .

Enhancement of Mutation Frequency with Nucleotide Triphosphate Analogs in PCR Random Mutagenesis. Hiroshi Ueda, Masaki Nakanishi, Eiji Suzuki, and Teruyuki Nagamune _. . . . . .

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