contents food separation of vitamin b12 in health food separation of carotenoids column mightysil...

01CONTENTS

01CONTENTS

F001 Separation of Water-soluble Vitamins - 1 05

F002 Separation of Water-soluble Vitamins - 2 05

F067 Separation of Vitamins 06

F005 Separation of Fat-soluble Vitamins 06

F070 Separation of Vitamin C 07

F003 Separation of Vitamin D2, D3 07

F057 Separation of Tocopherols 07

F058 Separation of Vitamin C and E 08

F097 Separation of Vitamin B12 in Health food 08

F071 Separation of Carotenoids 08

F004 Separation of β-Carotene in Broccoli 09

F059 Separation of β-Carotene 09

F007 Separation of Synthetic Antibiotics (Quinolone Derivatives)-1 09

F008 Separation of Synthetic Antibiotics (Quinolone Derivatives)-2 10

F009 Separation of Synthetic Antibiotics (Sulfa drugs) 10

F010 Separation of Synthetic Antibiotics (Furan Derivatives) 11

F011 Separation of Synthetic Antibiotics (Protozoicides) 11

F012 Separation of Synthetic Antibiotics (Protozoicide & Quinolone) 11

F013 Separation of 18 Synthetic Antibiotics 12

F026 Separation of Carbadox 13

F027 Separation of a Metabolite of Carbadox 13

F028 Separation of Sulfadimidine 13

F006 Separation of Tetracycline Antibiotics 13

F014 Separation of Drugs for Animals -1 14

F015 Separation of Drugs for Animals -2 14

F019 Analysis of Oxytetracyclne in Pork 14

F016 Analysis of α-Trenbolone in Pork 15

F017 Analysis of β-Trenbolone in Pork 15

F021 Analysis of Zeranol in Pork 15

F018 Analysis of lvermectin in Pork 15

F020 Analysis of Closantel in Pork 16

F022 Analysis of Flubendazole in Pork 16

F029 Separation of Thiabendazole 16

F030 Separation of a Metabolite of Thiabendazole 16

F023 Separation of Albendazole 17

F024 Separation of a Metabolite of Albendazole 17

F025 Separation of Isomethamidium 17

F031 Separation of Avoparcins 17

F044 Separation of Preservatives - 1 18






F061 Separation of Preservatives and Saccharin 20

F042 Separation of Antioxidants - 1 20

F073 Separation of Antioxidants - 2 20

F048 Separation of Ascorbic Acid & Erythorbic Acid 21

F045 Separation of anti-Fungal Preservatives - 1 21

F046 Separation of anti-Fungal Preservatives - 2 21

F037 Separation of Food Dyes - 1 21






F060 Separation of Anthocyanins 24

F050 Separation of Aspartame in a Beverage 24

F069 Separation of Acesulfame K 25

F080 Separation of Aspartame and Acesulfame K in breath mint 25

F049 Separation of Saccharin & Sorbic Acid 25

F076 Separation of Cyclamic acid 25

F051 Separation of Pyrazines 26

F089 Separation of Ubiquinone 9,10 26

F090 Separation of DL-Thioctic acid 26

F091 Separation of Benzoyl Peroxide in Flour 26

F053 Separation of Caffeine and Catechins 27

F054 Separation of Commercial Tea Drink 27

F055 Separation of Catechins 28

F056 Separation of Catechins, Caffeine, Chlorophyll a 28

F063 Separation of Allyl isothiocyanate 29

F064 Separation of Quassins 29

F065 Separation of Capsaicins 29

F082 Separation of Curcumin 29

F083 Separation of Curcumin in a commercial drink by LC/MS 30

F078 Separation of Rutin and Quercetin 30

F099 Separation of Apigenin 30

F100 Separation of 5,6-Benzoflavone 30

F101 Separation of 7,8-Benzoflavone 31

F102 Separation of Biochanin A 31

F103 Separation of Chrysin 31

F104 Separation of Diosmin 31

F105 Separation of Flavonol 32

F106 Separation of Hesperetin 32

F107 Separation of Hesperidin 32

F108 Separation of Isoquercitrin 32

F109 Separation of Kaempferol 33

F110 Separation of Luteolin 33

F111 Separation of Naringenin 33

F112 Separation of Naringin 33

F113 Separation of Rhoifolin 34

F114 Separation of 4,2',4'-Trihydroxychalcone 34

F066 Separation of Oligosaccharide 34

F068 Separation of Carbohydrates 34

F032 Separation of di-Carbonic Acids - 1 35

F092 Separation of di-Carbonic Acids - 2 35

F035 Separation of Organic Acids - 1 35

F093 Separation of Organic Acids – 2 35

F036 Separation of Organrc Acids - 3 36



F062 Separation of Formic acid and Tartaric acid 36

F033 Separation of Oxalic Acid and Nitric Acid in Spinach 37

F034 Separation of Aromatic Acids -1 37



F084 Separation of Malachite green and Leucomalachite green 38

F085 Separation of Malachite green and Leucomalachite green by LC/MS

38

F086 Separation of Sudan 38

F087 Separation of Sudan by LC/MS 39

F052 Separation of Aflatoxin G1, G2, B1, B2 39

F081 Separation of Fumonisins 40

F088 Separation of Melamine 40

F079 Separation of Putrefactive amines 40

SHEET NO. TITLE PAGE SHEET NO. TITLE PAGE

F O O D

C O N T E N T S

02 CONTENTS

E001 Separation of N-Methylcarbamate derivatives- 1 43

E002 Separatiori of N-Methylcarbamate derivatives- 2 43

E003 Separatron of Oxine-Copper and Asulam 44

E004 Separation of Oxine-Copper and Thiram 44

E005 Separation of Simazine, Thiram and Thiobencarb 44

E006 Separation of Thiram, Iprodione and Bensulide 44

E007 Separation of Thiram 45

E008 Separation of Thiophanate-methyl and its metabolite 45

E009 Separation of Fenitrothion and its metabolite 45

E011 Separation of Agricultural Chemicals (Germicides) 45

E012 Separation of Agricultural Chemicals (Pesticides) 46

E013 Separation of Agricultural Chemicals (Herbicides)- 1 46

E014 Separation of Agricultural Chemicals (Herbicides)- 2 46

E015 Separation of 5 Agricultural Chemicals - 1 46





E017 Separation of 6 Agricultural Chemicals 48





E045 Separation of Pesticides standard solution 24【A golf course pesticide】

50

E019 Separation of Intermediates of Agricultural Chemicals (halogenized Benzoic acids)

50

E027 Separation of Carbofuran 50

E032 Separation of Acephate 51

E033 Separation of Imazalil in Concentrated Lemonade Juice 51

E040 Separation of Pesticides standard solution 28 51


E042 Separation of Carbaryl 52

E043 Separation of Diquat 52

E044 Separation of Iminoctadine triacetate 52

E039 Separation of LAS 52

E010 Separation of 15 Synthetic Antibiotics 53

E020 Separation of Benzo[a]pyrene 53

E023 Separation of Microsystins 53

E046 Separation of Patulin 54

E024 Separation of Bisphenol A -1 54

E025 Separation of Bisphenol A -2 54

E026 Separation of Trace Level Bisphenol A 54

E031 Separation of 4-Vinylguaiacol 55

E021 Separation of DNPH-Aldehydes-1 55




SHEET NO. TITLE PAGE

E N V I R O N M E N T

C O N T E N T S

Concerning Mightysil RP-18 GP series

All columns with the "GP" title include the batch certificate.The guaranteed content is as follows.1. The physical properties of the packing materials, and carbon content after bonding2. Analytical results and Test-chromatograms of a separation with our own standard of test-mixtures

Difference in entitling "GP"or not is only including a batch certificate or not.In addition, they are made with the same packing material.

03FOOD

04 FOOD

05FOOD

Separation of Water-soluble Vitamins - 1

F001

F002

Separation of Water-soluble Vitamins - 2

Column Mightysil RP-18 150-4.6mmØ 5μm

Eluent Acetonitrile/ 20mM Phosphoric acid including 25mM of Sodiumhexanesulfonate = 10/ 90

Flow Rate 1.0mL/min.

Detection UV210nm 0.16AUFS

Temperature 40°C

Sample Vol. 5μL

Samples 1. Nicotinic acid 50μg/mL

2. Nicotinamide 50μg/mL

3. Riboflavin phosphate sodium salt 50μg/mL

4. Pyridoxine hydrochloride (Vitamin B6) 200μg/mL

5. Riboflavin (Vitamin B2) 150μg/mL

6. Folic acid (Vitamin B9) 50mg/mL

7. Thiamin hydrochloride(Vitamin B1) 150μg/mL


Eluent Acetonitrile/ 20mM Phosphoric acid including 25mM of Sodium hexanesulfonate = 10/ 90



Temperature 40°C

Sample Vol. 5μL

Samples 1. Pantothenic acid calcium salt 160μg/mL

2. Nicotinamide 20μg/mL

3. Pyridoxine hydrochloride (Vitamin B6) 40μg/mL

4. Riboflavin(Vitamin B2) 80μg/mL

06 FOOD

Separation of Vitamins

Separation of Fat-soluble Vitamins

Column Mightysil RP-18GP Aqua 250-4.6mmØ 5μm

Eluent

0.1% Phosphoric acid containing 10mM Sodium hexanesulfonate/ Acetonitrile = 90/10


Detection UV210nm

Temperature 35°C

Sample Vol. 5μL

Samples 1. Nicotinic acid 0.05mg/mL

2. Pantothenic acid Calcium salt 0.3 mg/mL

3. Nicotinamide 0.05mg/mL

4. Riboflavin phosphate Sodium salt 0.2 mg/mL

5. Pyridoxine hydrochloride(VitaminB6) 0.05mg/mL

6. Riboflavin (VitaminB2) 0.15mg/mL

7. Folic acid (VitaminB9) 0.05mg/mL

8. Thiamine hydrochloride 0.15mg/mL


Eluent Acetonitrile/ Methanol = 60/40



Temperature 40ºC

Sample Vol. 5μL

Samples 1. Menadione (Vitamin K3) 0.25mg/mL

2. Retinol acetate (Vitamin A acetate) 0.09mg/mL

3. Ergocalciferol (Vitamin D2) 0.10mg/mL

4. Cholecalciferol (Vitamin D3) 0.10mg/mL

5. Tocopherol (Vitamin E) 0.75mg/mL

6. Tocopherol acetate (Vitamin E acetate) 1.00mg/mL

7. Phylloquinone (Vitamin K1) 0.50mg/mL

F067

F005

07FOOD

Separation of Vitamin C Separation of Vitamin D2, D3

Separation of Tocopherols


Eluent 1% Methaphosphoric acid


Detection UV242nm

Temperature Ambient

Sample Vol. 5μL

Samples 1. L-Ascorbic acid 50mg/mL


Eluent Acetonitrile/ Water = 95/ 5



Temperature Room Temperature

Sample Vol. 5μL

Samples 1. Ergocalciferol (Vitamin D2) 250μg/mL

2. Cholecalciferol (Vitamin D3) 250μg/mL

Column Mightysil Si60 150-4.6mmØ 5μm

Eluent Hexane/ Dioxane/ Isopropylalcohol = 97.5/ 2/ 0.5


Detection Fluorescence 323nm (Excitation 296nm)

Temperature Ambient

Sample Vol. 5μL

Samples 1. α-Tocopherol 1μg/mL

2. 2,2,5,7,8-Pentamethylhydroxychroman 1μg/mL

3. β-Tocopherol 1μg/mL

4. γ-Tocopherol 1μg/mL

5. δ-Tocopherol 1μg/mL

F070 F003

F057

08 FOOD

Separation of Vitamin B12 in Health food

Separation of Carotenoids


Eluent Acetonitrile / 50mM Ammonium acetate = 10/90


Detection VIS550nm

Temperature 40°C

Sample Vol. 20μL

Samples Cyanocobalamin(Vitamin B12)

Column Mightysil RP-18(L)GP 150-4.6mmØ 5μm

Eluent Acetonitrile/ Methanol = 50/50

Flow rate 1.0mL/min.

Detection UV460nm

Temperature 40°C

Sample Vol. 10μL

Samples 1. Lutein 4mg/mL

2. β-Cryptoxanthin 2mg/mL

3. Lycopene 20mg/mL

4. β-Carotene 20mg/mL

F097

F071

Separation of Vitamin C and E

Column Mightysil RP-18GP 250-4.6mmØ 5μm

Eluent

A: Methanol/ 20mM Phosphoric acid = 1/ 99

B : Methanol/ Acetonitrile = 40/60

Gradient, Flow rate :

0~10min.: A100%, 1.0mL/min

10.1~30min.: B100%, 1.5mL/min

Detection UV254nm

Temperature 40°C

Sample Vol. 10μL

Samples 1. L-Ascorbic acid (Vitamin C) 0.2mg/mL

2. Tocopherol (Vitamin E) 0.2mg/mL

F058

09FOOD

Separation of β-Carotene in Broccoli Separation of β-Carotene

Separation of Synthetic Antibiotics (Quinolone Derivatives) - 1


Eluent Acetonitrile/ 50mM Sodium dihydrogenphosphate buffer (pH2.5*) = 35/ 65, including 3.5mM of Sodium dodecyl sulfate[*pH adjust : H3PO4]



Temperature 25°C

Sample Vol. 10μL (50ng)

Samples 1. Oxolinic acid 2. Nalidixic acid

3. Flumequine 4. Ofloxacin

5. Benofloxacin 6. Danofloxacin

7. Piromidic acid 8. Enrofloxacin

9. Orbifloxacin Each 5μg/mL


Eluent Chloroform/ Methanol = 20/80


Detection 453nm 0.005AUFS


Sample Vol. 10μL

Sample 1. β-Carotene

Standard: 0.5μg/mL Actual: Extraction in acetone


Eluent Ethanol 100%


Detection UV-VIS 453nm

Temperature 40°C

Sample Vol. 10μL

Samples 1. β-Carotene 2μg/mL

F004 F059

F007

10 FOOD

Separation of Synthetic Antibiotics (Quinolone Derivatives) - 2

Separation of Synthetic Antibiotics (Sulfa drugs)


Eluent Acetonitrile/ 25mM Sodium dihydrogenphosphate= 25/75



Temperature 40°C

Sample Vol. 5μL

Samples 1. Sulfisomidine 5μg/mL 2. Sulfathiazole 10μg/mL

3. Sulfamerazine 10μg/mL 4. Sulfamethoxypyridazine 10μg/mL

5. Sulfamonomethoxine 10μg/mL 6. Sulfachlorpyridazine 10μg/mL

7. Sulfamethoxazole 10μg/mL 8. Sulfadimethoxine 10μg/mL

F008

F009


Eluent Acetonitrile/ 50mM Sodium dihydrogenphosphate buffer (pH 2.4*) including 2.5mM of Sodium heptanesulfonate = 20/ 80 [*pH adjust : conc.H3PO4]


Detection Fluorescence 445nm ( Excitation 295nm)

Temperature 40°C

Sample Vol. 5μL

Samples 1. Danofloxacin mesylate

2. Enrofloxacin

11FOOD

Separation of Synthetic Antibiotics (Furan Derivatives) Separation of Synthetic Antibiotics (Protozoicides)

Separation of Synthetic Antibiotics (Protozoicide & Quinolone)

F010 F011

F012





Temperature 40°C

Sample Vol. 5μL

Samples 1. Nitro furazone 10μg/mL

2. Furazolidone 10μg/mL


Eluent Acetonitrile/ 10mM Potassium dihydrogenphosphate including 5mM of Sodium heptanesulfonate = 20/80



Temperature 40°C

Sample Vol. 5μL

Samples 1. Amprolium 13.75μg/mL

2. Oxolinic acid 3.75μg/mL





Temperature 40°C

Sample Vol. 5μL

Samples 1. Amprolium 15μg/mL

2. Pyrimethamine 5μg/mL

3. Ethopabate 5μg/mL

4. Nicarbazin 65μg/mL

12 FOOD

Separation of 18 Synthetic Antibiotics


Eluent

Acetonitrile/ 25mM Potassium dihydrogenphosphate= 15/ 85 (Eluent A), = 70/30 (Eluent B)



Temperature 40°C

Sample Vol. 10μL

Samples ODX: Olaquindox OMP: Ormethoprim OXA: Oxolinic acid

1μg/mL each SMR: Sulfamerazine SMM: Sulfamonomethoxine PYR: Pyrimethamine

CLP: Clopidol CDX: Carbadox DFZ: Difurazone

FZD: Furazolidone MRT: Morantel citrate NAA: Nalidixic acid

TMP: Trimethoprim SDM: Sulfadimethoxine PMA: Piromidic acid

SDD: Sulfadimidine SQX: Sulfaquinoxaline NCZ: Nicarbazin

F013


13FOOD

Separation of Carbadox Separation of a Metabolite of Carbadox

Separation of Sulfadimidine Separation of Tetracycline Antibiotics

F026 F027

F028 F006


Eluent Acetonitrile/ 25mM Potassium dihydrogenphosphate= 1/9


Detection UV310nm

Temperature 40°C

Sample Vol. 2μL

Sample 1. Carbadox 10μg/mL


Eluent Acetonitrile/ 25mM Sodium dihydrogenphosphate = 3/17


Detection UV268nm

Temperature 40°C

Sample Vol. 20μL

Sample 1. Sulfadimidine 0.5μg/mL


Eluent Acetonitrile/ 50mM Phosphate buffer(pH2.5) = 25/75



Temperature 25°C

Sample Vol. 10μL (25ng)

Samples 1. Oxytetracycline

2. Tetracycline

3. Chlortetracycline

4. Doxycycline


Eluent Acetonitrile/ 10mM Potassium dihydrogenphosphate buffer (pH2.5*)= 1/ 4 [*pH adjust : conc.H3PO4]


Detection UV320nm

Temperature 40°C

Sample Vol. 5μL

Sample 1. 2-Quinoxalinecarboxylic acid 50μg/mL

14 FOOD

Separation of Drugs for Animals – 1


Eluent

A: Methanol/ 25mM of potassium dihydrogenphosphate = 20/80B: Methanol/ 25mM of potassium dihydrogenphosphate = 95/5Linear gradient from 100% of A to 100% of B for 25 min.


Detection UV254nm

Temperature 40°C

Sample Vol. 20μL

Samples 1. Oxytetracycline 50μg/mL

2. Flubendazole 5μg/mL

3. β-Trenbolone 20μg/mL

4. α-Trenbolone 20μg/mL

5. Zeranol 20μg/mL

6. Closantel 10μg/mL

7. Ivermectin 20μg/mL

F014

Separation of Drugs for Animals – 2 Analysis of Oxytetracycline in Pork

F015 F019


Eluent Acetonitrile/ Phosphate buffer pH3.0 = 15/85



Temperature 40°C

Sample Vol. 5μL

Samples 1. 5-Hydroxythiabendazole 5μg/mL

2. 5-Propylsulfonyl-1H-benzimidazole-2-amine 5μg/mL

3. 2-Quinoxalinecarboxylic acid 15μg/mL

4. Sulfadimidine 7μg/mL


Eluent 1M of Imidazole buffer(pH7.2)/ Methanol = 4/1



Temperature 40°C

Sample Vol. 20μL

Sample 0.1ppm of Oxytetracycline was added to pork

15FOOD

Analysis of Zeranol in Pork

Analysis of α-Trenbolone in Pork

Analysis of Ivermectin in Pork

Analysis of β-Trenbolone in Pork

F021

F016

F018

F017


Eluent Methanol/ Water = 95/5



Temperature 40°C

Sample Vol. 20μL

Sample 0.02ppm of Ivermectin was added to pork

Column Mightysil RP-18 150-4.6mmØ 5mm

Eluent Acetonitrile/ Water = 5/6


Detection UV230nm

Temperature 40°C

Sample Vol. 100μL

Sample Actual pork (muscles)




Detection UV340nm

Temperature 40°C

Sample Vol. 100μL

Sample 0.01ppm of α-Trenbolone was added to pork




Detection UV340nm

Temperature 40°C

Sample Vol. 100μL

Sample 0.02ppm of β-Trenbolone was added to pork

16 FOOD

Separation of Thiabendazole

Analysis of Closantel in Pork

Separation of a Metabolite of Thiabendazole

Analysis of Flubendazole in Pork

F029

F020

F030

F022




Detection UV298nm

Temperature 40°C

Sample Vol. 100μL

Sample 1. 5-Hydroxythiabendazole 0.05μg/mL





Temperature 40°C

Sample Vol. 1μL

Sample 1. Thiabendazole 0.25μg/mL


Eluent

Methanol/ 20mM Sodium dihydrogenphosphate buffer(pH3.3*) = 7/ 2 [*pH adjust : H3PO4]


Detection UV369nm

Temperature 50°C

Sample Vol. 20μL

Sample 1ppm of Closantel was added to pork




Detection UV313nm

Temperature 40°C

Sample Vol. 20μL

Sample 0.01ppm of Flubendazole was added to pork

17FOOD

Separation of Isomethamidium

Separation of Albendazole

Separation of Avoparcins

Separation of a Metabolite of Albendazole

F025

F023

F031

F024


Eluent Acetonitrile/ 30mM Citric acid including 5mM of Sodium heptanesulfonic acid = 3/7


Detection UV380nm

Temperature 40°C

Sample Vol. 15μL

Sample 1. Isometamidium 0.1μg/mL


Eluent Acetonitrile/ 2.5% Acetate buffer(pH4.0*) including 10mM of Sodium heptanesulfonate = 11.5/ 88.5 [*pH adjust : NH4OH]


Detection UV280nm

Temperature Room Temperature (22°C)

Sample Vol. 20μL

Sample Control material including α-Avoparcin and β-Avoparcin




Detection Fluorescence 320nm (Excitation 280nm) 0.14AUFS

Temperature 40°C

Sample Vol. 1μL

Sample 1. 5-Propylsulfonyl-1H-benzimidazole-2-amine 0.5μg/mL





Temperature 40°C

Sample Vol. 20μL

Sample 1. Albendazole 0.01mg/mL

18 FOOD

Separation of Presevatives - 3

Separation of Preservatives - 1


Separation of Presevatives -2

F075

F044

F043

F047




Detection UV254nm

Temperature 40°C

Sample Vol. 10μL

Samples 1. Methyl p-hydroxybenzoate 1μg/mL

2. Ethyl p-hydroxybenzoate 1μg/mL

3. Isopropyl p-hydroxybenzoate 1μg/mL

4. n-Propyl p-hydroxybenzoate 1μg/mL

5. Isobutyl p-hydroxybenzoate 1μg/mL

6. n-Butyl p-hydroxybenzoate 1μg/mL


Eluent Acetonitrile/ 20mM Phosphate buffer(pH4.6) = 1/2




Sample Vol. 20μL

Samples 1. Benzoic acid 20μg/mL

2. Sorbic acid 20μg/mL

3. Dehydro acetic acid 20μg/mL


Eluent Acetonitrile/ 20mM Buffers pH4.6 = 1/2




Sample Vol. 20μL



3. Dehydroacetic acid 20μg/mL


Eluent 5mM Citric acid buffer*/ Acetonitrile/ Methanol = 70/ 20/10[*0.7g/L Citric acid monohydrate + 0.6g/L Trisodium citrate dehydrate]


Detection UV230nm

Temperature 40°C

Sample Vol. 10μL



3. Salicylic acid 100μg/mL

4. Dehydroacetic acid 100μg/mL

19FOOD


F074


Eluent

Methanol/ 5mM Citric acid buffer* = 50/50[*0.7g/L Citric acid monohydrate + 0.6g/L Trisodium citrate dihydrate]


Detection UV270nm

Temperature 40°C

Sample Vol. 20μL

Samples 1. Methyl p-hydroxybenzoate 4.0μg/mL

2. Ethyl p-hydroxybenzoate 4.0μg/mL

3. Isopropyl p-hydroxybenzoate 4.0μg/mL

4. Propyl p-hydroxybenzoate 4.0μg/mL

5. Isobutyl p-hydroxybenzoate 4.0μg/mL

6. Butyl p-hydroxybenzoate 4.0μg/mL

Separation of Presevatives - 6


Eluent Ethanol/ 50mM Sodium dihydrogen phosphate dihydrate = 34/ 66, including 2mM of Cetyltrimethylammonium bromide (pH4.41*) [*pH adjust : 10% Phosphoric acid, after mixing the components of eluent.]



Temperature 45°C

Sample Vol. 5μL

Samples 1. Ethyl p-hydroxybenzoate 0.1mg/mL

2. Sorbic acid 0.1mg/mL

3. Benzoic acid 0.1mg/mL

4. Isopropyl p-hydroxybenzoate 0.1mg/mL

5. Propyl p-hydroxybenzoate 0.1mg/mL

6. Saccharin 0.1mg/mL

7. Isobutyl p-hydroxybenzoate 0.1mg/mL

8. Butyl p-hydroxybenzoate 0.1mg/mL

F077

20 FOOD

Separation of Preservatives and Saccharin Separation of Antioxidants

F061 F042


Eluent 0.1% Pottasium phosphate buffer*/ Acetonitrile = 95/5[*0.1% Pottasium dihydrogen phosphate / 0.1% Dipotassium hydrogen phosphate = 1/1]


Detection UV225nm

Temperature 40°C

Sample Vol. 10μL



3. Saccharin 10μg/mL






Sample Vol. 10μL

Samples 1. Butylhydroquinone (BHQ) 100μg/mL

2. Butylhydroxyanisole (BHA) 100μg/mL

3. Butyl hydroxytoluene (BHT) 100μg/mL

Separation of Antioxdants - 2


Eluent A: Acetonitrile/ Methanol = 50/50B: 0.5% Acetic acidGradient (A%) :0~25min. : A45%~100% Linear, 25.1~30min.: A 100%, 30.1~50min.: A45%


Detection UV280nm

Temperature 40°C

Sample Vol. 10μL

Samples 1. Propyl gallate (PG) 40μg/mL

2. Trihydroxybutyrophenone (THBP) 40μg/mL

3. Butyl hydroquinone (BHQ) 40μg/mL

4. Nordihydroguaiaretic acid (NDGA) 40μg/mL

5. Butyl hydroxyanisole (BHA) 40μg/mL

6. Hydroxy methyl butyl phenol (HMBP) 40μg/mL

7. Octyl gallate (OG) 40μg/mL

8. Butyl hydroxytoluene (BHT) 40μg/mL

9. Dodecyl gallate (DG) 40μg/mL

F073

21FOOD

Separation of anti-Fungal Preservatives - 2

Separation of Ascorbic Acid & Erythorbic Acid

Separation of Food Dyes - 1

Separation of anti-Fungal Preservatives - 1

F046

F048

F037

F045


Eluent Acetonitrile/ 0.05% Potassium phosphate buffer* = 6/4 [*0.05% Potassium dihydrogen phosphate/0.05% Dipotassium hydrogen phosphate = 1/1]



Temperature 40°C

Sample Vol. 5μL

Samples 1. Thiabendazole (TBZ) 1μg/mL

2. o-Phenylphenol (OPP) 1μg/mL

3. Biphenyl (BP) 1μg/mL


Eluent

A: Methanol/ 10mM Ammonium Dihydrogenphosphate = 1/9B: Methanol/ 10mM Ammonium Dihydrogenphosphate = 8/2Linear gradient from 100% of A to 100% of B for 25min.




Sample Vol. 10μL

Samples 1. Tartrazine (Yellow-4) 40μg/mL

2. Sunset Yellow FCF (Yellow-5) 60μg/mL





Temperature 40°C

Sample Vol. 3μL

Samples 1. Thiabendazole (TBZ) 160μg/mL

2. o-Phenylphenol (OPP) 40μg/mL

3. Imazalil (IMZ) 70μg/mL

4. Biphenyl (BP) 50μg/mL


Eluent 50mM Disodium hydrogen phosphate buffer(pH6.8*) including 5mM of Tetrabutylammonium hydrogensulfate


Detection UV254nm

Temperature 40°C

Sample Vol. 1μL

Samples 1. Ascorbic acid 1mg/mL

2. Erythorbic acid 1mg/mL

22 FOOD




Eluent



Detection UV254nm 0.16AUFS (A), UV612nm 0.64AUFS (B)


Sample Vol. 20μL

Samples 1. Indigocarmine (Blue-2) 40μg/mL

2. Fast Green FCF (Green-3) 30μg/mL

3. Brilliant Blue FCF (Blue-1) 30μg/mL

F038

F039


Eluent

A: Methanol/ 10mM Ammonium Dihydrogenphosphate = 1/9B: Methanol/ 10mM Ammonium Dihydrogenphosphate = 8/2Linear gradient from 100% of A to 100% of B for 25min., then keeping 100% of B.




Sample Vol. 10μL

Samples 1. Amaranth (R2) 40μg/mL 2. New Coccine (R102) 40μg/mL

3. Acid Red (R106) 40μg/mL 4. Eosin Y 40μg/mL

5. Erythrosine (R3) 40μg/mL 6. Phloxine (R104) 40μg/mL

7. Rose Bengal (R105) 60μg/mL

23FOOD




Eluent



Detection

UV428nm (0-10min.); UV630nm (10-20min.); UV254nm (20-30min.) 0.08AUFS


Sample Vol. 20μL

Samples 1. Tartrazine Aluminium Lake 50μg/mL

2. Brilliant Blue Aluminium Lake 50μg/mL

3. Erythrosine Aluminium Lake 50μg/mL

F040

F041


Eluent

A: Methanol/ 10mM Ammonium Dihydrogenphosphate = 1/9B: Methanol/ 10mM Ammonium Dihydrogenphosphate = 8/2Linear gradient from 100% of A to 100% of B for 25min., then keeping 100% of B.




Sample Vol. 20μL

Samples 1. Tartrazine (Y4) 20μg/mL 2. Amaranth (R2) 20μg/mL

3. Indigocarmine (B2) 30μg/mL 4. New Coccine (R102) 20μg/mL

5. Sunset Yellow FCF (Y5) 30μg/mL 6. Fast Green FCF (G3) 50μg/mL

7. Brilliant Blue FCF (B1) 50μg/mL 8. Acid Red (R106) 20μg/mL

9. Eosin Y 20μg/mL 10. Erythrosine (R3) 20μg/mL

11. Phloxine (R104) 50μg/mL 12. Rose Bengal (R105) 30μg/mL

24 FOOD



Eluent A: Methanol/ Acetonitrile/ Water = 3/3/4 Containing Ammonium acetate solution(5g/100mL)B: Ammonium acetate solution(5g/100mL)Gradient : 0~30min. : A5%-A100% Linear, 30~36min.: A100%, 36.1~50min.: A5%


Detection UV254nm

Temperature 40°C

Sample Vol. 20μL

Samples 1. Tartrazine (Y4) 2. Amaranth (R2)

3. Indigocarmine (B2) 4. New Coccine (R102)

5. Sunset Yellow FCF (Y5) 6. Allura Red AC (R40)

7. Fast Green FCF (G3) 8. Brilliant Blue FCF (B1)

9. Eosin 10. Erythrosine (R3)

11. Acid Red (R106) 12. Phloxine (R104)

13. Rose Bengal (R105) (Each Sample: 20μg/mL)

F072

Separation of Anthocyanins Separation of Aspartame in a Beverage

F060 F050


Eluent

Methanol/ 33mM Phosphate buffer(pH4.9*) = 3/7 [*pH adjust : H3PO4]




Sample Vol. 2μL

Sample Actual beverage:

1. Benzoic acid

2. Caffeine

3. Aspartame


Eluent

A : 0.5% Trifluoroacetic acidB : Trifluoroacetic acid/ Acetonitrile = 50/50


Detection UV-VIS 520nm

Temperature 40°C

Sample Vol. 10μL

Samples 1. Acylanthocyanin 1mg/mL

2. Anthocyanin 3mg/mL

25FOOD

Separation of Saccharin & Sorbic Acid

Separation of Acesulfame K

Separation of Cyclamic acid

Separation of Aspartame and Acesulfame K in breath mint

F049

F069

F076

F080


Eluent

Acetonitrile/ 0.1% Potassium phosphate buffer* = 5/95 [*0.1% Potassium dihydrogen phosphate/ 0.1% Dipotassium hydrogen phosphate = 1/1 ]


Detection UV230nm

Temperature 40°C

Sample Vol. 10μL

Samples 1. Saccharin 10μg/mL





Detection UV314nm

Temperature 40°C

Sample Vol. 20μL

Samples 1. Cyclamic acid 20μg/mL

Column Mightysil RP-18 GP 150-4.6mmØ 5μm

Eluent Methanol/ 10mM Potassium dihydrogenphosphate(pH4.0*) including 10mM of Tetra-n-propylammonium hydroxide = 25/75[*pH adjust : H3PO4]


Detection DAD (200〜 400nm)

Temperature 40°C

Sample Vol. 10μL

Samples 1. Aspartame

2. Acesulfame K

Each 0.1mg/mL in Water

Commercial breath mint:

5 tablets of breath mint dissolved to 10mL of Water and filtered with 0.2μm PVDF membrane.


Eluent Acetonitrile/ 10mM Tetrabutylammonium dihydrogenphosphate = 40/60


Detection UV227nm

Temperature 40°C

Sample Vol. 20μL

Samples 1. Acesulfame K 0.01mg/mL

2. Ethyl-p-hydroxybenzoate 0.01mg/mL

26 FOOD

Separation of Pyrazines

Separation of DL-Thioctic acid

Separation of Ubiquinone 9, 10

Separation of Benzoyl Peroxide in Flour

F051

F090

F089

F091


Eluent Acetonitrile/ 10mM Potassium dihydrogenphosphate = 3/7




Sample Vol. 5μL

Samples 1. Pyrazine

2. 2-Methylpyrazine

3. 2,5-Dimethylpyrazine

4. 2,3,5-Trimethylpyrazine

5. 2,3,5,6-Tetramethylpyrazine


Eluent Methanol/ Ethanol = 13/7


Detection UV275nm

Temperature 35°C

Sample Vol. 5μL

Samples 1. Ubiquinone 9 (Coenzyme Q9)

2. Ubiquinone 10 (Coenzyme Q10)

Total of 500ppm

Column Mightysil RP-18GP 250-4.6mmØ　5μm

Eluent Acetonitrile / Water = 55/45


Detection UV235nm

Temperature 40°C

Sample Vol. 20μL

Preparation The Acetonitrile is added to flour , and filtered


Eluent Methanol/ Acetonitrile/ 0.005mol/L Phosphate Buffer (pH3.08*)=1160/180/920[*pH adjust : dil.H3PO4]


Detection UV215nm

Temperature 35°C

Sample Vol. 20μL

Samples DL-Thioctic acid 1mg/mL

( 0.005mol/L Phosphate buffer pH3.5/Acetonitrile=1/1.)

27FOOD

Separation of Caffeine and Catechins

F053


Eluent

A: 0.1% Phosphoric acid including 5% of N,N-Dimethylformamide and 0.1% of AcetonitrileB: Acetonitrile



Temperature 43°C

Sample Vol. 5μL (Standards), 3μL (Actual sample): Extracted with mixture of Acetonitrile and Water

Samples 1. Caffeine 0.10mg/mL in STD.

2. Epigallocatechin 0.05mg/mL in STD.

3. Catechin 0.05mg/mL in STD

4. Epicatechin 0.05mg/mL in STD.

5. Epigallocatechin Gallate 0.05mg/mL in STD

6. Epicatechin Gallate 0.05mg/mL in STD.

Separation of Commercial Tea drink

F054


Eluent A : Acetonitrile/ Water/ Phosphoric acid = 10/ 400/ 0.2 B : Methanol/ Acetonitrile/ Water/ Phosphoric acid = 200/ 10/ 400/ 0.2A 100% : 0~3min., A100%~A0%(B100%) : 3~25min., B 100% : 25~26min.


Detection UV230nm

Temperature 40°C

Sample Vol. 10μL (Commercial green tea drink)

Samples 1. Gallic acid 2. Theogallin

3. Catechin gallate 4. Epigallocatechin

5. Catechin 6. Caffeine

7. Epigallocatechin gallate 8. Epicatechin

9. Gallocatechin gallate 10. Epicatechin gallate

11. Catechin gallate

28 FOOD

Separation of Catechins

Separation of Catechins, Caffeine, Chlorophyll A

F055

F056


Eluent

A: 0.1% Phosphoric acid containing 0.1% Acetonitrile and 5% N,N-DimethylformamideB : Acetonitrile


Detection UV280nm

Temperature 40°C

Sample Vol. 5μL

Samples 1. Caffeine 2. Epigallocatechin

3. Catechin 4. Epicatechin

5. Epigallocatechin gallate 6. Gallocatechin gallate

7. Epicatechin gallate 8. Catechin gallate


Eluent

A: 1% Phosphoric acid, B : EthanolTime (min.) 0 0.1 17 20 35A 95 90 80 5 5B 5 10 20 95 95


Detection 0~21min. : UV280nm, 21~35min.: UV430nm

Temperature 40°C

Sample Vol. 5μL

Samples 1. Epigallocatechin 20μg/mL 2. Catechin 20μg/mL

3. Caffeine 4μg/mL 4. Epicatechin 20μg/mL

5. Epigallocatechin gallate 10μg/mL 6. Gallocatechin gallate 6μg/mL

7. Epicatechin gallate 8μg/mL 8. Catechin gallate 6μg/mL

9. Chlorophyll a 6μg/mL

29FOOD

Separation of Allyl isothiocyanate Separation of Quassins

F063 F064



Flow Rate 0.8 mL/min.

Detection UV240nm

Temperature 40°C

Sample Vol. 10μL

Samples 1. Allyl isothiocyanate 0.4mg/mL

2. Fenethylcyanate 0.3mg/mL




Detection UV254nm

Temperature 30°C

Sample Vol. 10μL

Samples 1.2. : Quassin 100μg/mL

3. p-Methylacetophenon 12.5μg/mL

Separation of Capsaicins Separation of Curcumin

F065 F082


Eluent Acetonitrile/ Water/ Acetic acid = 43/ 57/ 1

Flow Rate 1.2 mL/min.

Detection UV280nm

Temperature 45°C

Sample Vol. 15μL

Samples 1. Capsaicin 160μg/mL

2. Dihydrocapsaicin 80μg/mL


Eluent Acetonitrile/20mM Formic acid = 45/55


Detection UV425nm

Temperature 40°C

Sample Vol. 5μL

Samples Curcumin 0.1mg/mL

Commercial drink

The sample solution filtered with PVDF filter, after having diluted the commercial drink to 3 times with Acetonitrile.

30 FOOD

Separation of Apigenin

Separation of Curcumin in a commercial drink by LC/MS

Separation of 5,6-Benzoflavone

Separation of Rutin and Quercetin

F099

F083

F100

F078


Eluent Acetonitrile/ 25mM Potassium dihydrogenphosphate buffer(pH2.4*) = 30/70 [*pH adjust : H3PO4]


Detection UV340nm

Temperature 40ºC

Sample Vol. 50μL

Samples 1. Apigenin 0.02mg/mL




Detection UV270nm

Temperature 40ºC

Sample Vol. 10μL

Sample 1. 5,6-Benzoflavone 0.2mg/mL


Eluent Acetonitrile/20mM Formic acid = 45/55


Detection MS ESI-

Temperature 40°C

Sample Vol. 5μL

Samples Commercial drink

The sample solution filtered with PVDF filter, after having diluted the commercial drink to 3 times with Acetonitrile.


Eluent 2.5% Acetic acid/ Methanol/ Acetonitorile = 70/ 10/ 20


Detection UV350nm

Temperature 30°C

Sample Vol. 10μL

Samples 1. Rutin 0.8mg/mL

2. Quercetin 0.4mg/mL

31FOOD

Separation of 7,8-Benzoflavone Separation of Biochanin A

F101 F102

Columns Mightysil RP-18GP 150-4.6mmØ 5μm



Detection UV280nm

Temperature 40ºC

Sample Vol. 10μL

Sample 1. 7,8-Benzoflavone 0.2mg/mL


Eluent

Acetonitrile/ 25mM Potassium dihydrogenphosphate buffer(pH2.4*) = 50/50 [*pH adjust : H3PO4]


Detection UV260nm

Temperature 40ºC

Sample Vol. 5μL

Sample 1. Biochanin A 0.5mg/mL

Separation of Chrysin Separation of Diosmin

F103 F104




Detection UV265nm

Temperature 40ºC

Sample Vol. 5μL

Sample 1. Chrysin 0.1mg/mL


Eluent



Detection UV254nm

Temperature 40ºC

Sample Vol. 5μL

Sample 1. Diosmin 0.1mg/mL

32 FOOD

Separation of Hesperidin

Separation of Flavonol

Separation of Isoquercitrin

Separation of Hesperetin

F107

F105

F108

F106




Detection UV240nm

Temperature 40ºC

Sample Vol. 10μL

Sample 1. Flavonol 0.2mg/mL


Eluent



Detection UV285nm

Temperature 40ºC

Sample Vol. 5μL

Sample 1. Hesperetin 0.5mg/mL


Eluent



Detection UV285nm

Temperature 40ºC

Sample Vol. 10μL

Sample 1. Hesperidin 0.1mg/mL


Eluent



Detection UV254nm

Temperature 40ºC

Sample Vol. 5μL

Sample 1. Isoquercitrin 0.5mg/mL

33FOOD

Separation of Kaempferol Separation of Luteolin

F109 F110




Detection UV360nm

Temperature 40ºC

Sample Vol. 20μL

Sample 1.Kaempferol 0.2mg/mL




Detection UV345nm

Temperature 40ºC

Sample Vol. 20μL

Sample 1.Luteolin 0.1mg/mL

Separation of Naringenin Separation of Naringin

F111 F112




Detection UV290nm

Temperature 40ºC

Sample Vol. 20μL

Sample 1.Naringenin 0.2mg/mL




Detection UV280nm

Temperature 40ºC

Sample Vol. 10μL

Sample 1. Naringin 0.05mg/mL

34 FOOD

Separation of Oligosaccharide

Separation of Rhoifolin

Separation of Carbohydrates

Separation of Trihydroxychalcone

F066

F113

F068

F114




Detection UV330nm

Temperature 40ºC

Sample Vol. 5μL

Sample 1. Rhoifolin 0.03mg/mL




Detection UV370nm

Temperature 40ºC

Sample Vol. 10μL

Sample 1. 4,2,4-Trihydroxychalcone 0.5mg/mL


Eluent 100% Water


Detection RI

Temperature 35°C

Sample Vol. 5μL

Samples 1. Glucose

2. Maltose

3. Maltotriose

4. Maltotetraose

5. Maltopentaose

6. Maltohexaose

7. Maltoheptaose Each 1.0mg/mL


Eluent 100% Water


Detection RI

Temperature 35°C

Sample Vol. 5μL

Samples 1. Glucose 1.0mg/mL

2. Sucrose 1.0mg/mL

3. Raffinose 1.0mg/mL

35FOOD

Separation of di-Carbonic Acids-1 Separation of di-Carbonic Acids-2

F032 F092


Eluent Acetonitrile/ 20mM Phosphoric acid = 10/90




Sample Vol. 20μL

Samples 1. Malonic acid 150μg/mL

2. Succinic acid 200μg/mL

3. Glutaric acid 200μg/mL

4. Adipic acid 200μg/mL

5. Pimelic acid 250μg/mL


Eluent Acetonitrile/20mM Phosphoric acid = 10/90

Flow Rate 1.0mL / min.



Sample Vol. 20μL

Samples 1. Malonic acid 150μg/mL

2. Succinic acid 200μg/mL

3. Glutaric acid 200μg/mL

4. Adipic acid 200μg/mL

5. Pimelic acid 250μg/mL

Separation of Organic Acids - 1 Separation of Organic Acids – 2

F035 F093


Eluent 50mM Ammonium dihydrogenphosphate buffer (pH2.0*) [*pH adjust : conc.H3PO4]




Sample Vol. 5μL

Samples 1. Oxalic acid 0.1mg/mL

2. Glycolic acid 2.0mg/mL

3. Malic acid 2.0mg/mL

4. Lactic acid 3.0mg/mL

5. Citric acid 2.0mg/mL


Eluent Ammonium dihydrogenphosphate buffer(pH2.0)




Sample Vol. 5μL

Samples 1. Oxalic acid 0.1mg/mL

2. Glycolic acid 2.0mg/mL

3. Malic acid 2.0mg/mL

4. Lactic acid 3.0mg/mL

5. Citric acid 2.0mg/mL

36 FOOD

Separation of Organic Acids - 3 Separation of Organic Acids - 4

F036 F094


Eluent

50mM Ammonium dihydrogenphosphate buffer(pH2.0*) [*pH adjust : conc.H3PO4]




Sample Vol. 5μL

Samples 1. Oxalic acid 20μg/mL 5. Maleic acid 75μg/mL

2. Tartaric acid 300μg/mL 6. Citric acid 400μg/mL

3. Malic acid 350μg/mL 7. Fumaric acid 75μg/mL

4. Malonic acid 500μg/mL 8. Acrylic acid 75μg/mL


Eluent Ammonium dihydrogenphosphate buffer(pH2.0)




Sample Vol. 5μL

Samples 1. Oxalic acid 20μg/mL 5. Maleic acid 75μg/mL

2. Tartaric acid 300μg/mL 6. Citric acid 400μg/mL

3. Malic acid 350μg/mL 7. Fumaric acid 75μg/mL

4. Malonic acid 500μg/mL 8. Acrylic acid 75μg/mL

Separation of Organic Acids -5 Separation of Formic acid and Tartaric acid

F098 F062


Eluent 25mM Ammonium phosphate buffer (pH6.8) including 5mM of Tetrabutylammonium hydrogen sulphate



Temperature 40°C

Sample Vol. 10μL

Samples 1. Formic acid 0.7mg/mL

2. Tartaric acid 0.3mg/mL


Eluent 20mM Ammonium dihydrogenphosphate buffer(pH2.48)


Detection UV210nm

Temperature 35°C

Sample Vol. 20μL

Samples 1. Glycolic acid 100μg/mL 6. Maleic acid 1μg/mL

2. Malic acid 100μg/mL 7. Citric acid 100μg/mL

3. Malonic acid 100μg/mL 8. Fumaric acid 0.7μg/mL

4. Lactic acid 100μg/mL 9. Succinic acid 100μg/mL

5. Acetic acid 100μg/mL 10.Acrylic acid 1μg/mL

37FOOD

Separation of Oxalic Acid and Nitric Acid in Spinach Separation of Aromatic Acids-1

F033 F034


Eluent 5mM Tetrabutylammonium hydrogen sulfate in 50mM of Diammonium hydrogenphosphate buffer(pH6.8*) [*pH adjust : H3PO4]




Sample Vol. 20μL

Sample Actual spinach, extracted 5g of spinach solution was diluted with 250mL of water and filtered(1.Oxalic acid 2.Nitric acid )


Eluent Acetonitrile/ 20mM phosphoric acid = 2/ 8




Sample Vol. 10μL

Samples 1. Gallic acid 40μg/mL

2. Protocatechuic acid 20μg/mL

3. Phthalic acid 100μg/mL

4. Gentisic acid 100μg/mL


Separation of Aromatic Acids -2 Separation of Aromatic Acids -3

F096 F095


Eluent Acetonitrile/ 20mM phosphoric acid = 2/8




Sample Vol. 10μL


2. Protocatechuic acid 20μg/mL 20μg/mL





Eluent Acetonitrile/ 20mM phosphoric acid = 1.5/8.5




Sample Vol. 10μL


2. Protocatechuic acid 20μg/mL 20μg/mL




38 FOOD

Separation of Malachite green and Leucomalachite green Separation of Malachite green and Leucomalachite green by LC/MS

F084 F085


Eluent A: 0.01mol/L Ammonium formateB: Acetonitrile


Detection DAD: 580nm (0-13min.), 265nm (13-45min.)

Temperature 40°C

Sample Vol. 10μL

Samples 1. Malachite green 0.05mg/mL

2. Leucomalachite green 0.05mg/mL

Column Mightysil RP-18MS 150-2.0mmØ 5μm

Eluent

A: 0.01mol/L Ammonium formateB: Acetonitrile


Detection MS ESI+

Temperature 40°C

Sample Vol. 2μL

Samples 1. Malachite green 0.05mg/mL

2. Leucomalachite green 0.05mg/mL

Separation of Sudan

F086


Mightysil RP-18GP 150-4.6mmØ 5μm

Eluent A : Acetonitrile/ Methanol=85/15

B : Acetonitrile/ 0.1(v/v%)Formic acid=90/10


Detection UV500nm

Temperature 40°C

Sample Vol. 10μL

Samples 1. Para red

2. SudanⅠ

3. SudanⅡ

4. SudanⅢ

5. SudanⅣ Each 5μg/mL

39FOOD

Separation of Sudan by LC/MS

F087

Column Mightysil RP-18MS 150-2.0mmØ 5μm

Eluent Acetonitrile/ 0.1%(v/v)Formic acid =


Detection MS ESI+

Temperature 40°C

Sample Vol. 10μL

Samples 1. Para red 2. SudanⅠ

3. SudanⅡ 4. SudanⅢ

5. SudanⅣ Each 5μg/mL

Separation of Aflatoxin G1, G2, B1, B2

F052






Sample Vol. 20μL

Samples Mixture of samples were pre-treated with trifluoroacetic acid in order to convert G1 and B1 to their derivatives

1. Aflatoxin G1 derivative 20ng/mL

2. Aflatoxin B1 derivative 20ng/mL

3. Aflatoxin G2 20ng/mL

4. Aflatoxin B2 20ng/mL

40 FOOD

Separation of Fumonisins

F081

Column Mightysil RP-18 MS 150-2.0mmØ 5μm

Eluent A:Acetonitrile

B:0.1%Formic acid

Linear gradient:

0min A25%→ 5min A50%→ 8min A50%→ 10min A25%→ 25min A25%


Detection MS ESI+

Temperature 40°C

Sample Vol. 2μL

Samples 1. Fumonisin B1

2. Fumonisin B3

3. Fumonisin B2 Each 50ppb in Acetonitrile/Water= 50/ 50

Separation of Melamine Separation of Rutrefactive amines

F088 F079

Column Mightysil RP-8 GP 250-4.6mmØ 5μm

Eluent Buffer(pH 3.0)*/Acetonitrile=85/15[*10 mM Citric acid + 10 mM Sodium octane sulfonate, pH adjust : NaOH]


Detection UV240nm

Temperature 40°C

Sample Vol. 10μL

Samples 1. Ammelide

2. Ammeline

3. Melamine Each 0.1mg/mL


Eluent Acetonitorile /Water = 60/40



Temperature 40°C

Sample Vol. 5μL

Samples 1. Putrescine 10μg/mL

2. Cadaverine 10μg/mL

3. Histamine 250μg/mL

4. Tyramine 50μg/mL

5. Spermidine 10μg/mL

I.S : 1,6-Hexanediamine

41ENVIRONMENT

42 ENVIRONMENT

43ENVIRONMENT

Separation of N-Methylcarbamate derivatives-1

Separation of N-Methylcarbamate derivatives-2

E001

E002


Eluent A: Water, B: Acetonitrile


Detection

Fluorescence 445nm(Excitation 339nm)

Temperature 40°C

Sample Vol. 10μL

Samples 1. Aldicarb sulfoxide

2. Oxamyl

3. Methomyl

4. Ethiofencarb sulfoxide

5. Ethiofencarb sulfone

6. Carbofuran-3-keto

7. Carbofuran

8. Ethiofencarb

9. Isoprocarb

10. Methiocarb


Eluent A: Water B: Tetrahydrofuran C: Acetonitrile


Detection

Fluorescence 445nm(Excitation 339nm)

Temperature 40°C

Sample Vol. 10μL

Samples 1. Aldicarb sulfone

2. Ethiofencarb sulfone

3. Aldicarb

4. Propoxur

5. Carbofuran

6. Bendiocarb

7. Carbaryl

Condition of post labeling

Reaction Coil 5m-0.5mmØTeflon

Hydrolyzed by 0.05M NaOHsolution

Reagents Mixture of 10mg/L o-phthalaldehydein 50mM of Na2B4O7 solution and 50μL/L of Mercaptoethanol


Reaction Temperature

80°C

Condition of post labeling

Reaction Coil 5m-0.5mmØTeflon

Hydrolyzed by 0.05M NaOHsolution

Reagents Mixture of 10mg/L o-phthalaldehydein 50mM of Na2B4O7 solution and 50μL/L of Mercaptoethanol


Reaction Temperature

80°C

44 ENVIRONMENT

Separation of Oxine-Copper and Asulam Separation of Oxine-Copper and Thiram

Separation of Simazine, Thiram and Thiobencarb Separation of Thiram, Iprodione and Bensulide


Eluent Acetonitrile/ 20mM Phoshoric acid = 5/95




Sample Vol. 10μL

Samples 1. Oxine-Copper 0.08μg/mL

2. Asulam 0.40μg/mL


Eluent Acetonitrile/ 20mM Ammonium acetate buffer (pH3.80*)[*pH adjust : conc.Acetic acid] = 25/75



Temperature 40°C

Sample Vol. 10μL

Samples 1. Oxine-Copper 3μg/mL

2. Thiram 7μg/mL


Eluent Acetonitrile/ 20mM Phosphate buffer(pH3.0)= 55/45



Temperature 30°C

Sample Vol. 20μL

Samples 1. Simazine 1μg/mL

2. Thiram 1μg/mL

3. Thiobencarb 1μg/mL






Sample Vol. 10μL

Samples 1. Thiram 4μg/mL

2. Iprodione 10μg/mL

3. Bensulide 10μg/mL

E003 E004

E005 E006

45ENVIRONMENT

Separation of Thiram Separation of Thiophanate-methyl and its metabolite

Separation of Fenitrothion and its metabolite Separation of Agricultural Chemicals (Germicides)





Temperature 40°C

Sample Vol. 10μL

Sample 1. Thiram 0.4μg/mL


Eluent Methanol/ 10mM Potassium dihydrogenphosphate = 1/1


Detection UV278nm

Temperature 40°C

Samples 1. Methyl-2-benzimidazole carbamate

2. Thiophanate-methyl




Detection UV254nm

Temperature 40°C

Sample Vol. 50μL

Samples 1. Nitrocresol 500μg/mL

2. Fenitrothion 500μg/mL


Eluent Acetonitrile/ 10mM Potassium dihydrogenphosphate (pH3.5) = 55/45




Sample Vol. 10μL

Samples 1. Thiophanate-methyl 25μg/mL

2. Thiram 100μg/mL

3. Diclomezine 50μg/mL

4. Triflumizole 25μg/mL

5. Pencycuron 100μg/mL

E007 E008

E009 E011

46 ENVIRONMENT

Separation of Agricultural Chemicals (Pesticides) Separation of Agricultural Chemicals (Herbicides)-1

Separation of Agricultural Chemicals (Herbicides)-2 Separation of 5 Agricultural Chemicals -1






Sample Vol. 10μL

Samples 1. Imidacloprid 25μg/mL

2. Fenobucarb 100μg/mL

3. Hexythiazox 300μg/mL

4. Fluvalinate 60μg/mL


Eluent Acetonitrile/ 10mM Potassium dihydrogenphosphate (pH3.5) = 65/35




Sample Vol. 12μL

Samples 1. Dicamba 20μg/mL

2. Bensulfuron-methyl 30μg/mL

3. Mefenacet 10μg/mL

4. Bromobutide 150μg/mL

5. Pyributicarb 50μg/mL






Sample Vol. 7.5μL

Samples 1. Diuron 25μg/mL

2. Propanil 25μg/mL

3. Dymron (Daimuron) 75μg/mL

4. Chlorpropham 250μg/mL

5. Pyrazolate 125μg/mL


Eluent A: Acetonitrile/10mM Potassium dihydrogenphosphate (pH2.2) = 5/95B: Acetonitrile/10mM Potassium dihydrogenphosphate (pH2.2) = 80/20Linear gradient from 100% of A to 100% of B for 30 min.




Sample Vol. 3μL


2. Asulam 100μg/mL

3. Thiram 15μg/mL


5. Bensulide 200μg/mL

E012 E013

E014 E015

47ENVIRONMENT

Separation of 5 Agricultural Chemicals -2 Separation of 5 Agricultural Chemicals - 3

Separation of 5 Agricultural Chemicals – 4 Separation of 5 Agricultural Chemicals – 5


Eluent Acetonitrile/ 10mM Ammonium acetate buffer(pH3.3*)= 7/ 13 [*pH adjust : conc.Acetic acid]


Detection UV270nm(0-3min.), UV240nm(3-5.5min.), UV230nm(5.5-14min.)

Temperature 40°C

Sample Vol. 5μL

Samples 1. Asulam 10μg/mL

2. Oxine-Copper 10μg/mL

3. Triclopyr 10μg/mL

4. Thiram 10μg/mL

5. Mecoprop 10μg/mL

Column Mightysil RP-18 (L) GP 150-4.6mmØ 5μm

EluentAcetonitrile/ 10mM Pottasium dihydrogen phosphate Buffer (pH3.80*) = 1/3[*pH adjust : H3PO4]


Temperature 40°C

Sample Vol. 20μL

Samples 1. Asulam 270nm, 1μg/mL

Detection 2. Oxine-Copper 240nm, 1μg/mL

3. Mecoprop 230nm, 1μg/mL

4. Triclopyr 230nm, 1μg/mL

5. Thiram 270nm, 1μg/mL


Eluent Acetonitrile / Potassium Phosphate Buffer* = 50/50[*1.02g of Potassium dihydrogenphosphate was dissolved in 300mL water, then adjusted pH to 3.5 with H3PO4]


Detection UV230nm

Temperature 40°C

Sample Vol. 10μL


Detection 2. Mecoprop 5μg/mL

3. Benomyl 1μg/mL


5. Pencycuron 5μg/mL


Eluent A; Water, B; Acetonitrile C; Methanol


Detection UV270nm

Temperature 40°C

Sample Vol. 40μL

Samples 1. Imidacloprid 0.1μg/mL

2. Benomyl 0.1μg/mL

3. Thiophanate-methyl 0.1μg/mL

4. Carbaryl 0.1μg/mL

5. Linuron 0.1μg/mL

E016 E028

E029 E034

48 ENVIRONMENT

Separation of 12 Agricultural Chemicals


Eluent

Acetonitrile/ 10mM Pottasium dihydrogen phosphate Buffer (pH3.80*) = 1/3[*pH adjust : H3PO4]


Detection 0~6min.: 270nm, 6~10.9min.: 240nm, 10.9~24min.: 230nm, 24min.~: 270nm

Temperature 35°C

Sample Vol. 10μL

Samples 1. Asulam 2. Oxine-Copper

Detection 3. Simazine 4. Mecoprop

5. Thiram 6. Siduron

7. Propyzamide 8. Iprodione

9. Chlorothalonil 10. Pencycuron

11. Bensulide 12. Butamifos

Each 10μg/mL

E030

Separation of 6 Agricultural Chemicals Separation of 9 Agricultural Chemicals


Eluent A: Acetonitrile/ 10mM Potassium dihydrogenphosphate (pH2.2) = 5/95B: Acetonitrile/ 10mM Potassium dihydrogenphosphate (pH2.2) = 80/20Linear gradient from 100% of A to 100% of B for 30 min.




Sample Vol. 10μL

Samples 1. Oxine-Copper 20μg/mL 4. Thiram 20μg/mL

2. Asulam 120μg/mL 5. Iprodione 120μg/mL

3. Simazine 20μg/mL 6. Bensulide 240μg/mL


Eluent Acetonitrile Methanol/ 50mM Potassium dihydrogenphosphate (pH3.2) = 43/ 9/ 48


Detection 270nm(0-2.2min.), 240nm(2.2-10min.), 230nm(10-22min.),220nm(22-27min.), 240nm(27min.-)

Temperature 40°C

Sample Vol. 5μL

Samples 1. Asulam 10μg/mL 6. Chlorothalonil 10μg/mL

2. Oxine-Copper 10μg/mL 7. Iprodione 10μg/mL

3. Triclopyr 10μg/mL 8. Bensulide 10μg/mL

4. Thiram 10μg/mL 9. Pencycuron 10μg/mL

5. Mecoprop 10μg/mL

E017 E018

49ENVIRONMENT

Separation of 9 Agricultural Chemicals – 2

Separation of 3 Agricultural Chemicals


Eluent A; Water, B; Acetonitrile


Detection UV230nm

Temperature 40°C

Sample Vol. 10μL

Samples 1. hiabendazole 2. Triadimenol

3. Diflubenzuron 4. Bitertanol

5. Pyrazoxyfen 6. Triflumizole metabolite

7. Pyrazolate 8. Teflubenzuron

9. Chlorfluazuron Each 25μg/mL


Eluent A; Water, B; Acetonitrile


Detection UV230nm

Temperature 40°C

Sample Vol. 10μL

Samples 1. hiabendazole

2. Triadimenol

3. Diflubenzuron

4. Bitertanol

5. Pyrazoxyfen

6. Triflumizole metabolite

E035

E036


Eluent

A; 10mM Potassium dihydrogenphosphate(pH3.50*) [pH adjust :H3PO4]B; Acetonitrile



Temperature 40°C

Sample Vol. 10μL

Sample 1. Destosylpyrazolate 2.5μg/mL

2. Dithianon 2.5μg/mL

3. Triflumizole 2.5μg/mL

50 ENVIRONMENT

Separation of Intermediates of Agricultural Chemicals(halogenized Benzoic acids)

Separation of Carbofuran

E019 F027


Eluent Acetonitrile/ 0.1% Trifluoroacetic acid = 40/60




Sample Vol. 10μL

Samples

1mg/mL each





Temperature 40°C

Sample Vol. 50μL

Sample 1. Carbofuran 0.1μg/mL

Separation of Pesticides standard solution 24 [A golf course pesticide]

Column Mightysil RP-18PA 150-4.6mmØ 5μm

Eluent Acetonitrile/25mM Phosphate Buffer(pH3.3)=7/13


Detection UV270nm (0~2.5min), UV240nm (2.5~5.0min)

UV230nm (5.0~13.0min), UV240nm (13.0~16.0min)

UV255nm (16.0~19.5min), UV245nm (19.5~25.0min)

UV235nm (25.0~35.0min)

Temperature 40°C

Sample Vol. 5μL

Samples 34023-96 Pesticides standard solution 24

1. Asulam 2. Oxine-Copper

3. Triclopyr 4. Thiram

5. Mecoprop 6. Flazasulfuron

7. Siduron 8. Halosulfuron-methyl

9. Azoxystrobin

Each 10ppm

E045

51ENVIRONMENT

Separation of Acephate

Separation of Pesticides standard solution 28 Separation of 3 Agricultural Chemicals

Separation of Imazalil in Concentrated Lemonade Juice

E032

E040 E041

E033




Detection UV214nm

Temperature 40°C

Sample Vol. 20μL

Sample 1. Acephate 1mg/mL


Eluent Acetonitrile/ 50mM Potassium dihydrogenphosphate(pH3.3*) = 55/ 45[*pH adjust : H3PO4]


Detection UV270nm Asulam

UV230nm thiophanate-methyl, siduron, iprodion

Temperature 40°C

Sample Vol. 20μL

Samples 34027-96 Pesticides standard solution 28

1. Asulam 50ppm

2. Thiophanate-methyl 100ppm

3. Siduron 100ppm

4. Iprodione 50ppm

Cf. Complementary items (for water quality management) ; attached method 9


Eluent 2-Propanol/ Water = 30/70


Detection FL Ex : 339nm

Em : 455nm

Temperature 40°C

Post-column reaction sysytem

Reagent1: 50mM NaOH soln. 0.7mL/min

Reaction coil 0.5mmID×5m, 80°C

Reagent2: 6mM o-Phtalaldehyde

30mM 2-Mercaptoethanol

50mM Sodium Tetraborate

in Methanol/ Water= 1/ 100 0.7mL/min

Sample Vol. 1μL

Samples 1. Methomyl

2. Carbofuran

3. Carbaryl Each 10ppm

Cf. Complementary items(for water quality management) ; attached method 13


Eluent Methanol/ 20mM Disodium hydrogen phosphate dihydrate = 3/1


Detection UV230nm

Temperature 40°C

Sample Vol. 20μL

Sample 1. Imazalil 5mg/mL inLemonade Juice

52 ENVIRONMENT

Separation of LAS

E039


Eluent 0.1M Sodium perchlorate/ Acetonitrile= 65/3


Detection UV225nm

Temperature 40°C

Sample Vol. 10μL

Samples 24289-96 LAS standard stock solution 1000ppm (C10-14 total)

n-Decylbenzenesulfonic acid sodium salt (C10)

n-Undecylbenzenesulfonic acid sodium salt (C11)

n-Dodecylbenzenesulfonic acid sodium salt (C12)

n-Tridecylbenzenesulfonic acid sodium salt (C13)

n-Tetradecylbenzenesulfonic acid sodium salt (C14)

Separation of Carbaryl

Separation of Iminoctadine triacetate

Separation of Diquat

E042

E044

E043





Temperature 40°C

Sample Vol. 20μL

Samples 1. Carbaryl 1.0ppm



Eluent Acetonitrile/ 100mM Sodium perchlorate+0.05% Phosphoric acid in Water =5/17


Detection FL Ex 395nm, Em 500nm

Temperature 40°C

Post-column reaction sysytem

Reagent1: 500mM NaOH soln. 0.2mL/min

Reaction coil 0.5mmID×5m, 80°C

Reagent2: 3g/L Ninhydrin soln. 0.2mL/min

Sample Vol. 20μL

Samples 1. Iminoctadine triacetate 1.0ppm



Eluent 13.5mL/L Phosphoric acid + 3.0g/L Sodium 1-pentanesulfonate + 10mL/L Diethylamine in water


Detection UV313nm

Temperature 40°C

Sample Vol. 20μL

Samples 1. Diquat 1.0ppm


53ENVIRONMENT

Separation of Benzo[a]pyrene


Separation of Microsystins

E020

E010

E023


Eluent Methanol/ 50mM Phosphate Buffer(pH3.0) = 58/42


Detection UV240nm

Temperature Room Temperture

Sample Vol. 20μL

Samples 1. Microcystin RR 3.33μg/mL

2. Microcystin YR 3.33μg/mL

3. Microcystin LR 3.33μg/mL




Detection UV254nm

Temperature 40°C

Sample Vol. 3μL

Sample 1. Benzo[a]pyrene 2mg/mL


Eluent A: Methanol/ 25mM Potassium dihydrogenphosphate = 15/85

B: Methanol/ 25mM Potassium dihydrogenphosphate = 70/30


Detection Maximum wavelength detected by photodiode-array

Temperature 40°C

Sample Vol. 20μL

Samples QCA: 2-Quinoxaline CDX: Carbadox PYR: Pyrimethamine

carboxylic acid

SMR: Sulfamerazine MRT: Morantel citrate DFZ: Difurazone

FZD: Furazolidone SDM: Sulfadimethoxine NAA: Nalidixic acid

SDD: Sulfadimidine SQX: Sulfaquinoxaline PMA: Piromidic acid

SMM: Sulfamonomethoxine OXA: Oxolinic acid NCZ: Nicarbazin

54 ENVIRONMENT

Separation of Bisphenol A –2 Separation of Trace Level Bisphenol A

Separation of Patulin Separation of Bisphenol A -1

E025 E026

E046 E024





Temperature 40°C

Sample Vol. 100μL

Samples 1. Phenol 2.5ppm

2. Bisphenol A 2.5ppm

3. 4-tert-Butylphenol 2.5ppm



Flow rate 0.9mL/min

Detection UV225nm

Temperature 45°C

Sample Vol. 100μL

Samples 1. Phenol 0.01μg/mL

2. Bisphenol A 0.01μg/mL

3. 4-tert-Butylphenol 0.01μg/mL


Eluent Acetonitrile/0.082% Sodium perchlorate =3/97


Detection UV276nm

Temperature 40°C

Sample Vol. 50μL

Samples 1. Patulin 0.05ppm


Eluent A: Acetonitrile/ Water = 7/3B: AcetonitrileLinear gradient from 100% of A to 100% of B for 33min.


Detection UV217nm

Temperature 40°C

Sample Vol. 20μL

Sample 1. Bisphenol A 0.625ppm (Assayed value)

55ENVIRONMENT

Separation of DNPH-Aldehydes-2

Separation of 4-Vinylguaiacol Separation of DNPH-Aldehydes-1

E022

E031 E021




Detection UV385nm

Temperature 40°C

Sample Vol. 1μL

Samples 1. Formaldehyde-2,4-DNPH 100μg/mL

2. Acetaldehyde-2,4-DNPH 100μg/mL

3. Acrolein-2,4-DNPH 100μg/mL

4. Methylethylketone-2,4-DNPH 200μg/mL

5. Methylisobutylketone-2,4-DNPH 200μg/mL

6. Isophorone-2,4-DNPH 200μg/mL




Detection UV360nm

Temperature 40°C

Sample Vol. 20μL

Samples 1. Formaldehyde-2,4-DNPH 1μg/mL (as HCHO)

2. Acetoaldehyde-2,4-DNPH 1μg/mL (as CH3CHO)


Eluent Phosphate Buffer*/ Methanol = 50/50[*4.90g/L phosphoric acid/ 6.80g/L Pottasium dihydrogen phosphate = 1/2]


Detection UV260nm

Temperature 40°C

Sample Vol. 5μL

Sample 1. 4-Vinylguaiacol 19μg/mL

56 ENVIRONMENT



Column Mightysil DNPH 250-4.6mmØ 5μm

Eluent A: Water

B: Methanol


Detection UV360nm

Temperature 40°C

Sample Vol. 10μL

Samples 1. Formaldehyde-DNPH 6. Crotonaldehyde-DNPH

2. Acetaldehyde-DNPH 7. Butylaldehyde-DNPH

3. Acrolein-DNPH 8. Benzaldehyde-DNPH

4. Acetone-DNPH 9. iso-Valeraldehyde-DNPH

5. Propionaldehyde-DNPH 10. Valeraldehyde-DNPH

Each 1ppm

Column Mightysil DNPH 250-4.6mmØ 5μm

Eluent A: Water

B: Acetonitrile


Detection UV360nm

Temperature 40°C

Sample Vol. 10μL

Samples 1. Formaldehyde-DNPH 6. Crotonaldehyde-DNPH

Each 1ppm 2. Acetaldehyde-DNPH 7. Butylaldehyde-DNPH

3. Acrolein-DNPH 8. Benzaldehyde-DNPH

4. Acetone-DNPH 9. iso-Valeraldehyde-DNPH

5. Propionaldehyde-DNPH 10. Valeraldehyde-DNPH

01106-96 Aldehydes-2,4-DNPH standard stock solutionⅡ

E037

E038

57INDEX

AAcephate E032(51)

AcesulfameK F069(25),F080(25)

Acetaldehyde-2,4-DNPH E021(55),E022(55),E037(56),E038(56)

Aceticacid F098(36)

Acetone-2,4-DNPH E037(56),E038(56)

Acidred,Red-106 F039(22),F040(23),F072(24)

Acrolein-2,4-DNPH E022(55),E037(56),E038(56)

Acrylicacid F036(36),F094(36),F098(36)

Acylanthocyanin F060(24)

Adipicacid F032(35),F092(35)

AflatoxinB1 F052(39)

AflatoxinB2 F052(39)

AflatoxinG1 F052(39)

AflatoxinG2 F052(39)

Albendazole F023(17)

Aldicarb E002(43)

Aldicarbsulfone E002(43)

Aldicarbsulfoxide E001(43)

AlluraRedAC,Red-40 F072(24)

Allylisothiocyanate F063(29)

Amaranth,Red-2 F039(22),F040(23),F072(24)

Ammelide F088(40)

Ammeline F088(40)

Amprolium F011(11),F012(11)

Anthocyanin F060(24)

Apigenin F099(30)

Ascorbicacid F048(21)

L-Ascorbicacid,VitaminC F058(08),F070(07)

Aspartame F050(24),F080(25)

Asulam E003(44),E015(46),E016(47),E017(48),E018(48),

E028(47),E030(48),E040(51),E045(50)

Avoparcinmixture F031(17)

Azoxystrobin E045(50)

BBendiocarb E002(43)

Benofloxacin F007(09)

Benomyl E029(47),E034(47)

Bensulfuronmethyl E013(46)

Bensulide E006(44),E015(46),E017(48),E018(48),E030(48)

Benzaldehyde-2,4-DNPH E037(56),E038(56)

Benzo[a]pyrene E020(53)

5,6-Benzoflavone F100(30)

7,8-Benzoflavone F101(31)

Benzoicacid F044(18),F047(18),F050(24),F061(20),F075(18),F077(19)

Benzoylperoxide F091(26)

BHA,tert-Butylhydroxyanisole F042(20),F073(20)

BHQ,2-tert-Butylhydroquinone F042(20),F073(20)

BHT,Butylhydroxytoluene F042(20),F073(20)

BiochaninA F102(31)

Biphenyl F045(21),F046(21)

BisphenolA E024(54),E025(54),E026(54)

Bitertanol E035(49)

Blue-1,BrilliantblueFCF F038(22),F040(23),F072(24)

Blue-1AluminiumLake,BrilliantblueFCFAluminiumLake F041(23)

Blue-2,Indigocarmine F038(22),F040(23),F072(24)

BrilliantblueFCF,Blue-1 F038(22),F040(23),F072(24)

BrilliantblueFCFAluminiumLake,Blue-1AluminiumLake F041(23)

Bromobutide E013(46)

Butamifos E030(48)

Butylaldehyde-2,4-DNPH E037(56),E038(56)

2-tert-Butylhydroquinone,BHQ F042(20),F073(20)

Butylhydroquinone,2-tert-Butylhydroquinone F042(20),F073(20)

tert-Butylhydroxyanisole,BHA F042(20),F073(20)

Butylhydroxyanisole,tert-Butylhydroxyanisole F042(20),F073(20)

Butylhydroxytoluene,BHT F042(20),F073(20)

n-Butylparaben,n-Butylp-hydroxybenzoate F043(18),F074(19)

2,6-di-tert-Butyl-p-cresol,Butylhydroxytoluene F042(20),F073(20)

4-tert-Butylphenol E025(54),E026(54)

n-Butylp-hydroxybenzoate,n-Butylparaben F043(18),F074(19),F077(19)

CCadaverine F079(40)

Caffeine F050(24),F053(27),F054(27),F055(28),F056(28)

Capsaicin F065(29)

Carbadox E010(53),F013(12),F026(13)

Carbaryl E002(43),E034(47),E041(51),E042(52)

Carbofuran E001(43),E002(43),E027(50),E041(51)

Carbofuran-3-keto E001(43)

β-Carotene F004(09),F059(09),F071(08)

Catechin F053(27),F054(27),F055(28),F056(28)

Catechingallate F054(27),F055(28),F056(28)

Chlorfluazuron E035(49)

Chlorophylla F056(28)

Chlorothalonil E018(48),E030(48)

Chlorpropham E014(46)

Chlortetracycline F006(13)

Cholecalciferol,VitaminD3 F003(07),F005(06)

Chrysin F103(31)

Citricacid F035(35),F036(36),F093(35),F094(36),F098(36)

Clopidol F013(12)

Closantel F014(14),F020(16)

CoenzymeQ10,Ubiquinone10 F089(26)

CoenzymeQ9,Ubiquinone9 F089(26)

Crotonaldehyde-2,4-DNPH E037(56),E038(56)

β-Cryptoxanthin F071(08)

Curcumin F082(29),F083(30)

Cyanocobalamin,VitaminB12 F097(08)

Cyclamicacid F076(25)

DDaimuron,Dymron E014(46)

Danofloxacin F007(09)

I N D E XCONPOUNDNAME SHEETNO.(PAGE) CONPOUNDNAME SHEETNO.(PAGE)

58 INDEX

Danofloxacinmesylate F008(10)

n-Decylbenzenesulfonicacidsodiumsalt E039(52)

Dehydroaceticacid F044(18),F047(18),F075(18)

Destosylpyrazolate E036(49)

DG,Dodecylgallate F073(20)

Dicamba E013(46)

Diclomezine E011(45)

Diflubenzuron E035(49)

Difurazone E010(53),F013(12)

Dihydrocapsaicin F065(29)

2,5-Dimethylpyrazine F051(26)

Diosmin F104(31)

Diquat E043(52)

Dithianon E036(49)

Diuron E014(46)

n-Dodecylbenzenesulfonicacidsodiumsalt E039(52)

Dodecylgallate,DG F073(20)

Doxycycline F006(13)

Dymron,Daimuron E014(46)

EEnrofloxacin F007(09),F008(10)

Eosin F072(24)

EosinY F039(22),F040(23)

Epicatechin F053(27),F054(27),F055(28),F056(28)

Epicatechingallate F053(27),F054(27),F055(28),F056(28)

Epigallocatechin F053(27),F054(27),F055(28),F056(28)

Epigallocatechingallate F053(27),F054(27),F055(28),F056(28)

Ergocalciferol,VitaminD2 F003(07),F005(06)

Erythorbicacid F048(21)

Erythrosine,Red-3 F039(22),F040(23),F072(24)

ErythrosineAluminiumLake,Red-3AluminiumLake F041(23)

Ethiofencarb E001(43)

Ethiofencarbsulfone E001(43),E002(43)

Ethiofencarbsulfoxide E001(43)

Ethopabate F011(11)

Ethylp-hydroxybenzoate,Ethylparaben F043(18),F069(25),F074(19),

F077(19)

Ethylparaben,Ethylp-hydroxybenzoate F043(18),F069(25),F074(19),

F077(19)

FFastgreenFCF,Gleen-3 F038(22),F040(23),F072(24)

Fenethylcyanate F063(29)

Fenitrothion E009(45)

Fenobucarb E012(46)

Flavonol F105(32)

Flazasulfuron E045(50)

Flubendazole F014(14),F022(16)

Flumequine F007(09)

Fluvalinate E012(46)

Folicacid,VitaminB9 F001(05),F067(06)

Formaldehyde-2,4-DNPH E021(55),E022(55),E037(56),E038(56)

Formicacid F062(36)

Fumaricacid F036(36),F094(36),F098(36)

FumonisinB1 F081(40)



Furazolidone E010(53),F010(11),F013(12)

GGallicacid F034(37),F054(27),F095(37),F096(37)

Gallocatechingallate F054(27),F055(28),F056(28)

Gentisicacid F034(37),F095(37),F096(37)

Gleen-3,FastgreenFCF F038(22),F040(23),F072(24)

Glucose F066(34),F068(34)

Glutaricacid F032(35),F092(35)

Glycolicacid F035(35),F093(35),F098(36)

HHalogenatedbenzoicacid E019(50)

Halosulfuron-methyl E045(50)

Hesperetin F106(32)

Hesperidin F107(32)

1,6-Hexanediamine F079(40)

Hexythiazox E012(46)

Histamine F079(40)

HMBP,Hydroxymethylbutylphenol F073(20)

Hydroxymethylbutylphenol,HMBP F073(20)

5-Hydroxythiabendazole F015(14),F030(16)

IImazalil E033(51),F045(21)

Imidacloprid E012(46),E034(47)

Iminoctadinetriacetate E044(52)

Indigocarmine,Blue-2 F038(22),F040(23),F072(24)

Iprodione E006(44),E015(46),E017(48),E018(48),

E029(47),E030(48),E040(51)

Isobutylp-hydroxybenzoate,Isobutylparaben F043(18),F074(19),F077(19)

Isobutylparaben,Isobutylp-hydroxybenzoate F043(18),F074(19),F077(19)

Isometamidium F025(17)

Isophorone-2,4-DNPH E022(55)

Isoprocarb E001(43)

Isopropylp-hydroxybenzoate,Isopropylparaben F043(18),F074(19),F077(19)

Isopropylparaben,Isopropylp-hydroxybenzoate F043(18),F074(19),F077(19)

Isoquercitrin F108(32)

iso-Valeraldehyde-2,4-DNPH E037(56),E038(56)

Ivermectin F014(14),F018(15)

KKaempferol F109(33)

LLacticacid F035(35),F093(35),F098(36)

CONPOUNDNAME SHEETNO.(PAGE) CONPOUNDNAME SHEETNO.(PAGE)

I N D E X

59INDEX

LAS E039(52)

Leucomalachitegreen F084(38),F085(38)

Linuron E034(47)

Lutein F071(08)

Luteolin F110(33)

Lycopene F071(08)

MMalachitegreen F084(38),F085(38)

Maleicacid F036(36),F094(36),F098(36)

Malicacid F035(35),F036(36),F093(35),F094(36),F098(36)

Malonicacid F032(35),F036(36),F092(35),F094(36),F098(36)

Maltoheptaose F066(34)

Maltohexaose F066(34)

Maltopentaose F066(34)

Maltose F066(34)

Maltotetraose F066(34)

Maltotriose F066(34)

Mecoprop E016(47),E018(48),E028(47),E029(47),E030(48),E045(50)

Mefenacet E013(46)

Melamine F088(40)

Menadione,VitaminK3 F005(06)

Methiocarb E001(43)

Methomyl E001(43),E041(51)

p-Methylacetophenon F064(29)

Methyl-2-benzimidazolecarbamate E008(45)

Methylethylketone-2,4-DNPH E022(55)

Methylp-hydroxybenzoate,Methylparaben F043(18),F074(19)

Methylisobutylketone-2,4-DNPH E022(55)

Methylparaben,Methylp-hydroxybenzoate F043(18),F074(19)

2-Methylpyrazine F051(26)

Microcystin E023(53)

Morantelcitrate E010(53),F013(12)

NNalidixicacid E010(53),F007(09),F013(12)

Naringenin F111(33)

Naringin F112(33)

NDGA,Nordihydroguaiareticacid F073(20)

Newcoccine,Red-102 F039(22),F040(23),F072(24)

Nicarbazin E010(53),F011(11),F013(12)

Nicotinamide F001(05),F002(05),F067(06)

Nicotinicacid F001(05),F067(06)

Nitricacid F033(37)

Nitrocresol E009(45)

Nitrofurazone F010(11)

Nordihydroguaiareticacid,NDGA F073(20)

OOctylgallate,OG F073(20)

Ofloxacin F007(09)

OG,Octylgallate F073(20)

Olaquindox F013(12)

Orbifloxacin F007(09)

Ormethoprim F013(12)

Oxalicacid F033(37),F035(35),F036(36),F093(35),F094(36)

Oxamyl E001(43)

Oxine-copper E003(44),E004(44),E015(46),E016(47),E017(48),

E018(48),E028(47),E029(47),E030(48),E045(50)

Oxolinicacid E010(53),F007(09),F012(11),F013(12)

Oxytetracycline F006(13),F014(14),F019(14)

PPantothenicacidcalciumsalt F002(05),F067(06)

Parared F086(38),F087(39)

Patulin E046(54)

Pencycuron E011(45),E018(48),E029(47),E030(48)

2,2,5,7,8-Pentamethylhydroxychroman F057(07)

PG,Propylgallate F073(20)

Phenol E025(54),E026(54)

o-Phenylphenol F045(21),F046(21)

Phloxine,Red-104 F039(22),F040(23),F072(24)

Phthalicacid F034(37),F095(37),F096(37)

Phylloquinone,VitaminK1 F005(06)

Pimelicacid F032(35),F092(35)

Piromidicacid E010(53),F007(09),F013(12)

Propanil E014(46)

Propionaldehyde-2,4-DNPH E037(56),E038(56)

Propoxur E002(43)

Propylgallate,PG F073(20)

n-Propylp-hydroxybenzoate,n-Propylparaben F043(18),F074(19),F077(19)

n-Propylparaben,n-Propylp-hydroxybenzoate F043(18),F074(19),F077(19)

5-Propylsulfonyl-1H-benzimidazole-2-amine F015(14),F024(17)

Propyzamide E030(48)

Protocatechuicacid F034(37),F095(37),F096(37)

Putrescine F079(40)

Pyrazin F051(26)

Pyrazolate E014(46),E035(49)

Pyrazoxyfen E035(49)

Pyributicarb E013(46)

Pyridoxinehydrochloride,VitaminB6 F001(05),F002(05),F067(06)

Pyrimethamine E010(53),F011(11),F013(12)

QQuassin F064(29)

Quercetin F078(30)

2-Quinoxalinecarboxylicacid E010(53),F015(14),F027(13)

RRaffinose F068(34)

Red-102,Newcoccine F039(22),F040(23),F072(24)

Red-104,Phloxine F039(22),F040(23),F072(24)

Red-105,Rosebengal F039(22),F040(23),F072(24)

Red-106,Acidred F039(22),F040(23),F072(24)

CONPOUNDNAME SHEETNO.(PAGE) CONPOUNDNAME SHEETNO.(PAGE)

I N D E X

60 INDEX

Red-2,Amaranth F039(22),F040(23),F072(24)

Red-3,Erythrosine F039(22),F040(23),F072(24)

Red-3AluminiumLake,ErythrosineAluminiumLake F041(23)

Red-40,AlluraRedAC F072(24)

Retinolacetate,VitaminAacetate F005(06)

Rhoifolin F113(34)

Riboflavin,VitaminB2 F001(05),F002(05),F067(06)

Riboflavinphosphatesodiumsalt F001(05),F067(06)

Rosebengal,Red-105 F039(22),F040(23),F072(24)

Rutin F078(30)

SSaccharin F049(25),F061(20),F077(19)

Salicylicacid F034(37),F075(18),F095(37),F096(37)

Siduron E030(48),E040(51),E045(50)

Simazine E005(44),E017(48),E030(48)

Sorbicacid F044(18),F047(18),F049(25),F061(20),

F075(18),F077(19)

Spermidine

Succinicacid F032(35),F092(35),F098(36)

Sucrose F068(34)

SudanⅠ F086(38),F087(39)

SudanⅡ F086(38),F087(39)

SudanⅢ F086(38),F087(39)

SudanⅣ F086(38),F087(39)

Sulfachlorpyridazine F009(10)

Sulfadimethoxine E010(53),F009(10),F013(12)

Sulfadimidine E010(53),F013(12),F015(14),F028(13)

Sulfamerazine E010(53),F009(10),F013(12)

Sulfamethoxazole F009(10)

Sulfamethoxypyridazine F009(10)

Sulfamonomethoxine E010(53),F009(10),F013(12)

Sulfaquinoxaline E010(53),F013(12)

Sulfathiazole F009(10)

Sulfisomidine F009(10)

SunsetyellowFCF,Yellow-5 F037(21),F040(23),F072(24)

TTartaricacid F036(36),F062(36),F094(36)

Tartrazine,Yellow-4 F037(21),F040(23),F072(24)

TartrazineAluminiumLake,Yellow-4AluminiumLake F041(23)

Teflubenzuron E035(49)

Tetracycline F006(13)

n-Tetradecylbenzenesulfonicacidsodiumsalt E039(52)

2,3,5,6-Tetramethylpyrazine F051(26)

THBP,Trihydroxybutyrophenone F073(20)

Theogallin F054(27)

Thiabendazole E035(49),F029(16),F045(21),F046(21)

Thiaminehydrochloride,VitaminB1 F001(05),F067(06)

Thiobencarb E005(44)

DL-Thiocticacid F090(26)

Thiophanate-methyl E008(45),E011(45),E034(47),E040(51)

Thiram E004(44)E005(44),E006(44),E007(45),E011(45),

E015(46),E016(47),E017(48),E018(48),E028(47),E030(48),E045(50)

Tocopherol,VitaminE F005(06),F058(08)

α-Tocopherol F057(07)

β-Tocopherol F057(07)

γ-Tocopherol F057(07)

δ-Tocopherol F057(07)

Tocopherolacetate,VitaminEacetate F005(06)

α-Trenbolone F014(14),F016(15)

β-Trenbolone F014(14),F017(15)

Triadimenol E035(49)

Triclopyr E016(47),E018(48),E028(47),E045(50)

n-Tridecylbenzenesulfonicacidsodiumsalt E039(52)

Triflumizole E011(45),E036(49)

Triflumizolemetabolite E035(49)

Trihydroxybutyrophenone,THBP F073(20)

4,2',4'-Trihydroxychalcone F114(34)

Trimethoprim F013(12)

2,3,5-Trimethylpyrazine F051(26)

Tyramine F079(40)

UUbiquinone10,CoenzymeQ10 F089(26)

Ubiquinone9,CoenzymeQ9 F089(26)

n-Undecylbenzenesulfonicacidsodiumsalt E039(52)

VValeraldehyde-2,4-DNPH E037(56),E038(56)

4-Vinylguaiacol E031(55)

VitaminAacetate,Retinolacetate F005(06)

VitaminB1,Thiaminehydrochloride F001(05),F067(06)

VitaminB12,Cyanocobalamin F097(08)

VitaminB2,Riboflavin F001(05),F002(05),F067(06)

VitaminB6,Pyridoxinehydrochloride F001(05),F002(05),F067(06)

VitaminB9,Folicacid F001(05),F067(06)

VitaminC,L-Ascorbicacid F058(08),F070(07)

VitaminD2,Ergocalciferol F003(07),F005(06)

VitaminD3,Cholecalciferol F003(07),F005(06)

VitaminE,Tocopherol F005(06),F058(08)

VitaminEacetate,Tocopherolacetate F005(06)

VitaminK1,Phylloquinone F005(06)

VitaminK3,Menadione F005(06)

YYellow-4,Tartrazine F037(21),F072(24)

Yellow-5,SunsetyellowFCF F037(21),F040(23),F072(24)

ZZeranol F014(14),F021(15)

I N D E XCONPOUNDNAME SHEETNO.(PAGE) CONPOUNDNAME SHEETNO.(PAGE)

02 CONTENTS

contents food separation of vitamin b12 in health food separation of carotenoids column mightysil...

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