condition of coral reef fish larvae along the florida keys ......condition of coral reef fish larvae...
TRANSCRIPT
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Condition of coral reef fish larvae along the Florida Keys shelf:
Martha J. Hauff, Su Sponaugle, Robert K. CowenRosenstiel School of Marine and Atmospheric Science
University of Miami
Implications for connectivity
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Are reef fish populations open or closed?
Local Retention Panmixia
?
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Larval condition affects:
• Larval survival
• Juvenile survival
• Population connectivity
So . . .
. . . Do larvae with different
dispersal histories have
different condition?
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Three 18-day cruises on R/V Walton Smith June 2007 and August 2007, July 2008
Sampling Logistics
Paired MOCNESS and paired frame netSample discrete 20m depth bins (0-20, 20-40, 40-60, 60-80m)
Larvae sorted onboardTarget species cursorily identified and flash frozen
Environmental Data CollectionCTD
ADCP
Fluorescence
Transmittance
Dissolved oxygen
ARGOS drifters
Satellite imagery (SSH, ocean color)
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Key Largo
Big Pine Key
FLORIDA
Loop Current
Sampling Scheme
Marquesas
Long Key
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Measuring Condition
• ID and measure individual larvae, remove heads and guts and homogenize
• Use fluorometry to determine RNA/DNA
Clemmesen 1996
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Thalassoma bifasciatumBluehead wrasse
Pseudogramma gregoryiReef bass
Sphyraena barracudaGreat barracuda
Xyrichtys spp.Razorfish (pearly, rosy, green)
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Nearshore vs. Offshore Condition Thalassoma bifasciatum
a
b
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Nearshore vs. Offshore Condition Xyrichtys spp.
a
b
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Nearshore vs. Offshore Condition Pseudogramma gregoryi
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Nearshore vs. Offshore Condition Sphyraena barracuda
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Condition Among Transects
a
babab
ab
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Temporal Variability
27-29°C
28-30°C
28-31°C
a a
b
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Condition vs. Plankton Abundance
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Sponaugle et al. 2009
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SPECIFIC PREY ABUNDANCES
OTOLITH-DERIVED GROWTH DATA
BIOPHYSICAL MODELING
GUT CONTENTS
RNA/DNA
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Conclusions
Lower condition in offshore larvae
Lower larval condition in Loop Current and off of Key Largo, higher larval condition off of the
middle and lower Keys
Higher condition in 2008 than 2007
Larval dispersal trajectory may affect condition and thus, patterns of population replenishment
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Joel Llopiz
Laura Parsons
Katie Shulzitski
Claire Paris
Margie Oleksiak
Doug Crawford
Cedric Guigand
Claire Paris
Evan D’Alessandro
Dave Richardson
Adam Greer
Joan Holt
Danielle McDonald
Andy Bakun
Carie BicksonTom Murphy
Jason Downing
Capt. Shawn Lake and the crew of the
R/V F.G. Walton Smith
Rowlands Fellowship
Thank You:
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Searcy and Sponaugle 2001
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Cluster Analysis (June 2007)
N4
N5N6
O7
N14
N15
O18
N25
N26
O27
O28
N35
N36
O37
O38
N45
N46
O47
O49
N55N56
O57
O58O59
N64
N66
O67
O68O69
N74N75
N76
O77O78
O79
O80
O80A
O81O82
O83
O84
O90
O91
O92
O93
O94
O95
Z2Z3
Z4
Z5Z6
Z7
Z8
Z9
Z10
Z11
Z12
Z13
Z14
Z15Z16
Z17
Z18
Z19
Z20
Z21Z22
Z23
Z24
Z25
Z26
Z27
Z28
Z29Z30
Z31Z32Z33
Z34
Z35
Z36
Z37
Similarity (%)100 9698 9799
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Next Steps
o Analyze additional taxa• Data for five other taxa, and many more samples from
all three cruiseso Look at R/D ratio in relation to other environmental
variables • Prey field• Multivariate environmental analysis• Gut contents
o Compare R/D to other indices of condition• Growth rates (otoliths)• Lipid components analysis (TAG:Sterol)o Look at condition in relation to modeled larval origins
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Cluster Analysis (August 2007)
N4
N5
N6O7
O8O9
N14N15N16
O17
O18
O19
N24
N26
O27
O28
O29
N34N35N36
O37
O38
O39
N44
N45
N46
O47
O48
O49
N54
N55
N56
O57
O58
O59
N64
N65
N66
O67
O68
N74
N75
N76
O77
O78O79
O80O81
O82O83O85O86
O90
O91O92
O93
O94
O95
O96
X1X2
X3
X4
X5
X6
X7X8
X9
X10
X11
X12
X13
X14
X16
X17
X19X20X21
Similarity (%)100 9698 9799
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Environmental Data Collection
CTD
ADCP
Fluorescence
Transmittance
Dissolved oxygen
ARGOS drifters
Satellite imagery
(SSH, ocean color)
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Are reef fish populations open or closed?
Local Retention Panmixia
?Adult SpawningLarval Behavior/Swimming Ability
Larval MortalityPhysical Processes
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LC
FC
YUC
CUB
Gulf of Mexico
GS
TG