como construir un fotoreactor

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In this instructable, we describe how to build a photo-bioreactor that uses algae to

convert carbon dioxide and sunlight into energy. The energy that is produced is in the

form of algae biomass. The photo-bioreactor is built from plastic recycled water bottles.By designing the apparatus to be compartmentalized, we are able to do many
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experiments in parallel.

Michael ischer

mfischer !t stanford.edu

By using algae as a biofuel, we can increase the world"s supply of oil while at the same

time we decrease the amount of atmospheric carbon dioxide used during its production.

The resulting product is a sustainable biofuel whose carbon footprint is neutral

inasmuch as the #$% produced on consumption is essentially balanced by the #$% used

in its production. In this instructable, we first ma&e the carbon dioxide delivery system,

then mount the water bottles on a rac&, and then inoculate the bottles with algae. 'fter

letting the algae grow for a wee&, we extract the biomass.

Step 1 Make Carbon Dioxide Delivery System
http://www.instructables.com/id/An-Algae-Bioreactor-from-Recycled-Water-Bottles/step1/Make-Carbon-Dioxide-Delivery-System/http://cdn.instructables.com/F2R/2ZDG/FQMXBNJX/F2R2ZDGFQMXBNJX.LARGE.jpghttp://www.instructables.com/id/An-Algae-Bioreactor-from-Recycled-Water-Bottles/step1/Make-Carbon-Dioxide-Delivery-System/


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To ma&e the carbon dioxide delivery system, connect an eight port sprin&ler system

manifold to a one inch long ()# pipe. To get good seals, use Teflon tape to tape the

threads before attaching the pieces together. *ext, attach the one inch pipe to a T-

connector. Bloc& off one end of the T-connector and attach the other end to a foot long

()# pipe.

Step 2 Attach Tubing to Manifold
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or each manifold, cut eight-pieces of flexible tubing and connect each piece to a port

of the manifold. The manifold that I am using has a dial on each port to control the rate

of flow. Ma&e sure all the ports that you use are open and allow approximately the same

amount of carbon dioxide to flow through the port.

Step Mount Carbon Dioxide System
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Mount the air system to a metal rac& using zip ties. 'ttach the air system to a tan& of

carbon dioxide.

Step ! Mount "ater #ottles
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+ot glue the water bottles to the metal rac&.

Step $ Make Algae Media
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e next ma&e the medium to grow the algae. 'lthough there are many possible

mediums, a standard garden store fertilizer contains all the nitrogen and nutrients that

the algae need.

Step % Media &noculation
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' good source of algae is pond algae, if available. If not, there are a large number of

online vendors that sell batches of algae. To inoculate the culture, measure out a fixed

amount of algae and add it to the growth medium.

Step ' (ro)th and *arvesting
http://www.instructables.com/id/An-Algae-Bioreactor-from-Recycled-Water-Bottles/step7/Growth-and-Harvesting/http://cdn.instructables.com/FTZ/YL4M/FQMXBNJS/FTZYL4MFQMXBNJS.LARGE.jpghttp://cdn.instructables.com/F1N/JMAY/FQMXBNJT/F1NJMAYFQMXBNJT.LARGE.jpghttp://www.instructables.com/id/An-Algae-Bioreactor-from-Recycled-Water-Bottles/step7/Growth-and-Harvesting/


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'fter several days of sunlight and #$% exposure, the algae are much denser. ' rench

press is then used to extract the algae from the solution. The biomass of the dried algae

can then be used as a fuel. 's a by-product of this process, a large amount of

atmospheric #$% is seuestered.

By Michael ischer, /tanford 0niversity, mfischer !t stanford.edu

como construir un fotoreactor

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