communities of ectomycorrhizal fungi in a pb contaminated pine forest site nan hui, rauni strömmer,...
TRANSCRIPT
![Page 1: Communities of ectomycorrhizal fungi in a Pb contaminated pine forest site Nan Hui, Rauni Strömmer, Teija Koivula, Martin Romantschuk, Dept. of Ecological](https://reader035.vdocuments.mx/reader035/viewer/2022070413/5697bf9c1a28abf838c933df/html5/thumbnails/1.jpg)
Communities of ectomycorrhizal fungi in a Pb contaminated pine forest site
Nan Hui, Rauni Strömmer, Teija Koivula,
Martin Romantschuk,
Dept. of Ecological and Environmental Sciences, University of Helsinki, Niemenkatu 73, FI-15140 Lahti, Finland
![Page 2: Communities of ectomycorrhizal fungi in a Pb contaminated pine forest site Nan Hui, Rauni Strömmer, Teija Koivula, Martin Romantschuk, Dept. of Ecological](https://reader035.vdocuments.mx/reader035/viewer/2022070413/5697bf9c1a28abf838c933df/html5/thumbnails/2.jpg)
Hälvälä an old shotgun shooting range located at a pine
forest site, located in southern Finland, is used as the
study area.
Introduction
![Page 3: Communities of ectomycorrhizal fungi in a Pb contaminated pine forest site Nan Hui, Rauni Strömmer, Teija Koivula, Martin Romantschuk, Dept. of Ecological](https://reader035.vdocuments.mx/reader035/viewer/2022070413/5697bf9c1a28abf838c933df/html5/thumbnails/3.jpg)
Study areas
![Page 4: Communities of ectomycorrhizal fungi in a Pb contaminated pine forest site Nan Hui, Rauni Strömmer, Teija Koivula, Martin Romantschuk, Dept. of Ecological](https://reader035.vdocuments.mx/reader035/viewer/2022070413/5697bf9c1a28abf838c933df/html5/thumbnails/4.jpg)
Contamination Level
mg/kg
![Page 5: Communities of ectomycorrhizal fungi in a Pb contaminated pine forest site Nan Hui, Rauni Strömmer, Teija Koivula, Martin Romantschuk, Dept. of Ecological](https://reader035.vdocuments.mx/reader035/viewer/2022070413/5697bf9c1a28abf838c933df/html5/thumbnails/5.jpg)
Clean Polluted
![Page 6: Communities of ectomycorrhizal fungi in a Pb contaminated pine forest site Nan Hui, Rauni Strömmer, Teija Koivula, Martin Romantschuk, Dept. of Ecological](https://reader035.vdocuments.mx/reader035/viewer/2022070413/5697bf9c1a28abf838c933df/html5/thumbnails/6.jpg)
Mycorrhiza
A mycorrhiza is a symbiotic association between a fungus
and the roots of a plant.
root
![Page 7: Communities of ectomycorrhizal fungi in a Pb contaminated pine forest site Nan Hui, Rauni Strömmer, Teija Koivula, Martin Romantschuk, Dept. of Ecological](https://reader035.vdocuments.mx/reader035/viewer/2022070413/5697bf9c1a28abf838c933df/html5/thumbnails/7.jpg)
Types of Mycorrhizas
The two most common types ectomycorrhizas
Basidiomycota and Ascomycota endomycorrhizas (Arbuscular mycorrhizas)
associated with the roots of herbaceous plants
other mycorrhizas
![Page 8: Communities of ectomycorrhizal fungi in a Pb contaminated pine forest site Nan Hui, Rauni Strömmer, Teija Koivula, Martin Romantschuk, Dept. of Ecological](https://reader035.vdocuments.mx/reader035/viewer/2022070413/5697bf9c1a28abf838c933df/html5/thumbnails/8.jpg)
Pine root tips and Ectomycorrhizal fungi
1mm
![Page 9: Communities of ectomycorrhizal fungi in a Pb contaminated pine forest site Nan Hui, Rauni Strömmer, Teija Koivula, Martin Romantschuk, Dept. of Ecological](https://reader035.vdocuments.mx/reader035/viewer/2022070413/5697bf9c1a28abf838c933df/html5/thumbnails/9.jpg)
The main point of the study
Find the Ectomycorrhizal fungi species difference between Clean and Lead Polluted area.
Find the species which are particularly Lead tolerable.
Find the species which are Lead sensitive.
![Page 10: Communities of ectomycorrhizal fungi in a Pb contaminated pine forest site Nan Hui, Rauni Strömmer, Teija Koivula, Martin Romantschuk, Dept. of Ecological](https://reader035.vdocuments.mx/reader035/viewer/2022070413/5697bf9c1a28abf838c933df/html5/thumbnails/10.jpg)
Method and material
Total DNA
extraction
Amplifying certain area
of Fungal Ribosomal DNA by PCR
sequence the PCR product
Aligning the sequence with the
genebank database
![Page 11: Communities of ectomycorrhizal fungi in a Pb contaminated pine forest site Nan Hui, Rauni Strömmer, Teija Koivula, Martin Romantschuk, Dept. of Ecological](https://reader035.vdocuments.mx/reader035/viewer/2022070413/5697bf9c1a28abf838c933df/html5/thumbnails/11.jpg)
DNA Extraction from pine root tips
Root tips isolation
FastDNA SPIN Kit for soil
modified the original protocol
![Page 12: Communities of ectomycorrhizal fungi in a Pb contaminated pine forest site Nan Hui, Rauni Strömmer, Teija Koivula, Martin Romantschuk, Dept. of Ecological](https://reader035.vdocuments.mx/reader035/viewer/2022070413/5697bf9c1a28abf838c933df/html5/thumbnails/12.jpg)
PCR amplification
18S 5.8S 28SITS1 ITS2
Fun18f
ITS4
Fungal ribosomal RNA gene
Primer selection
![Page 13: Communities of ectomycorrhizal fungi in a Pb contaminated pine forest site Nan Hui, Rauni Strömmer, Teija Koivula, Martin Romantschuk, Dept. of Ecological](https://reader035.vdocuments.mx/reader035/viewer/2022070413/5697bf9c1a28abf838c933df/html5/thumbnails/13.jpg)
Sequencing and Bioinformation
In Biocenter Viikki, Helsinki
Staden Package
EMBL
A Nucleotide Sequence Database
http://www.ebi.ac.uk/embl/ UNITE
A molecular database for the identification of
ectomycorrhizal fungi
http://unite.ut.ee/
![Page 14: Communities of ectomycorrhizal fungi in a Pb contaminated pine forest site Nan Hui, Rauni Strömmer, Teija Koivula, Martin Romantschuk, Dept. of Ecological](https://reader035.vdocuments.mx/reader035/viewer/2022070413/5697bf9c1a28abf838c933df/html5/thumbnails/14.jpg)
Result
Altogether 351 sequences were distributed into 37
different groups. These groups were aligned to EMBL-EBI
database and UNITE database.
![Page 15: Communities of ectomycorrhizal fungi in a Pb contaminated pine forest site Nan Hui, Rauni Strömmer, Teija Koivula, Martin Romantschuk, Dept. of Ecological](https://reader035.vdocuments.mx/reader035/viewer/2022070413/5697bf9c1a28abf838c933df/html5/thumbnails/15.jpg)
The phylogenetic trees are made by neighborjoining method (PHYLIP).
Dermocystidium salmonis is used as an
outgroup. The tree colored according to the distribution of species in the three environmental conditions.
Clean: gray; Medium: magenta; Polluted(III): red;
Both in Medium and polluted:Yellow
The uncolored four species could be found in all the three environmental conditions.
![Page 16: Communities of ectomycorrhizal fungi in a Pb contaminated pine forest site Nan Hui, Rauni Strömmer, Teija Koivula, Martin Romantschuk, Dept. of Ecological](https://reader035.vdocuments.mx/reader035/viewer/2022070413/5697bf9c1a28abf838c933df/html5/thumbnails/16.jpg)
Fig.1 Species tree in the clean area
![Page 17: Communities of ectomycorrhizal fungi in a Pb contaminated pine forest site Nan Hui, Rauni Strömmer, Teija Koivula, Martin Romantschuk, Dept. of Ecological](https://reader035.vdocuments.mx/reader035/viewer/2022070413/5697bf9c1a28abf838c933df/html5/thumbnails/17.jpg)
Frequency of the species in different study areas
Clean
Medium Polluted
![Page 18: Communities of ectomycorrhizal fungi in a Pb contaminated pine forest site Nan Hui, Rauni Strömmer, Teija Koivula, Martin Romantschuk, Dept. of Ecological](https://reader035.vdocuments.mx/reader035/viewer/2022070413/5697bf9c1a28abf838c933df/html5/thumbnails/18.jpg)
Common species in all areas
Lactarius rufus
Piloderma fallax
Piloderma reticulatum
Piloderma croceum
Species found only in clean area
Tylospora asterophora
Tylospora fibrillosa
Russula decolorans
Mortierella verticillata*
Russula betularum
Species found only in polluted area
Tomentella stuposa
Suillus luteus
Cortinarius armeniacus
Nolanea conferenda
Thelephora terrestris
Amanita spissa
![Page 19: Communities of ectomycorrhizal fungi in a Pb contaminated pine forest site Nan Hui, Rauni Strömmer, Teija Koivula, Martin Romantschuk, Dept. of Ecological](https://reader035.vdocuments.mx/reader035/viewer/2022070413/5697bf9c1a28abf838c933df/html5/thumbnails/19.jpg)
Conclusion
Clear difference is found between clean and polluted
area.
In clean area, all the species are recognizable by
genebank database.
In polluted area, some unknown novel species are found
and given a number.
![Page 20: Communities of ectomycorrhizal fungi in a Pb contaminated pine forest site Nan Hui, Rauni Strömmer, Teija Koivula, Martin Romantschuk, Dept. of Ecological](https://reader035.vdocuments.mx/reader035/viewer/2022070413/5697bf9c1a28abf838c933df/html5/thumbnails/20.jpg)
Thanks for your attention