Packaging Solution using IGR Methoprene for Insect Control

• Commercially available Technology • Test Data from Lab and Field Studies

• Objective: Reduction in use of fumigants and lower spoilage and returns.

IGR (Insect Growth Regulator)

X X X

Methoprene is used extensively in Integrated Pest Management Programs

Adult

Pupa

X

Feed Products Purina Brand markets a “Feed-through” for Beef Cattle and Horses to control fly populations in feedlots

Grain Storage Diacon(Central Garden and Pet)- Markets a powdered additive to grain bins during harvest and transfer for insect control

Pet Flea and Tick Treatments Applied to pets to control fleas and ticks in Neck applications and collars

Food Manufacturing Facility Fogging solutions in warehouses and plants to control insect populations

Other Technology Applications for Insect Growth Regulators

Commercially Available Products

Film/Woven Packaging Solution PGT IGR 30000 Film Integration Additive •Liquid additive to film making process

• Combines and resides in films • Manually combines with Pellets • Can be metered directly in extruder • Fully Compliant w/ all regulatory agencies • No Odor or Taste • Adds insect control

Paper Packaging Solution PGT 23207 Water Based Coating

•Clear water based topcoat • Typically printed over graphic images • Replaces existing coatings • Duplicates existing coating in

COF Gloss Rub Resistance

• Fully Compliant w/ all regulatory agencies • Adds insect control

Controls for over 1 year….

FDA Status ProvisionGardTM complies with FDA requirements for Food, Feed & Tobacco packaging EPA Status ProvisionGard™ is registered with the EPA (registration # 81390-1) CONEG (Coalition of Northeastern Governors) ProvisionGard™ complies with the requirements of the toxics in packaging law(s)

ProvisionGard Regulatory Status

Test Results

All data generated using USDA

Protocols and trained Entomologists.

7

Evaluation of ProvisionGard for Control of Flour Beetles and Indian Meal Moth in Paper and

Woven Poly Bags

Go to www.pvgard.com to watch video

Jade Vardeman

Research Entomologist Moses Biologic, LLC

Treated vs Untreated Cartons with Water Based Coating

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ProvisionGard Study Breakfast Cereal Cartons

September 2009

PG Treated

Control

Rice Packaged in Treated Cartons and Treated Corrugate Boxes

CBR Cartons (18 cartons/shipper)

Control Treated

Total # of shippers 14 10

Total # of insects 329 0

Average # insects/shipper 23.5 0.0

Range of insect count/shipper 0 – 132 0 – 0

Average reduction in treated vs. control 100% efficacy

Brown Rice Cartons (18 cartons/shipper)

Control Treated Total # of shippers 8 11 Total # of insects 60 5 Average # insects/shipper 7.5 0.5 Range of insect count/shipper 0 – 37 0 – 3 Average reduction in treated vs. control 93.4% efficacy

CBR Poly (6 bags/shipper) In Corrugate Boxes

Control Treated

Total # of Shippers 28 28

Total # of Insects 182 3

Average # of insects/shipper 6.5 0.1

Range of insect count/shipper 2 – 13 0 – 1

Average Reduction in Treated vs. Control 99% efficacy

Whole Grain 5# Poly (12 bags/shipper)in Corrugate Box

Control Treated

Total # of Shippers 10 15

Total # of Insects 95 0

Average # of insects/shipper 9.5 0

Range of insect count/shipper

1– 23 0 – 0

Average Reduction in

Treated vs. Control 100% efficacy

“Blinded” Pet Food: Multiwall Bags

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1 11 21 31 41 51 61 71 81 91 101 111 121

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Evaluation of ProvisionGard for Control of Flour Beetles

Packaging- Film Woven Poly

Dr. Frank Arthur

Research Entomologist USDA-ARS-CGAHR

Manhattan, KS 66502

Methods-Test 1 • Circular disks of packaging cut to fit bottom portion of a 9.6 in2 plastic Petri dish

• Disks with inner and outer portions of the packaging

• Ten 4-week old red flour beetle larvae or ten confused flour beetle larvae of same age put in separate dishes with 300 mg flour

Treated Dish-left, Untreated-right

Results to Date

• Confused flour beetle the more tolerant of the two species (consistent with other studies)

• Outer portion still active after 8 months

• Inner portion losing efficacy

Inner Outer

Month 0 5.0 ± 2.0 0.0 ± 0.0

Month 2 3.3 ± 2.1 1.6 ± 1.6

Month 4 23.3 ± 3.3 5.0 ± 3.4

Month 6? 66.7 ± 21.1 6.7 ± 3.3

Month 8 30.0 ± 12. 1 0.0 ± 0.0

• Conclusions

% Emergence-Red Flour Beetle

Inner Outer

Month 0 41.7 ± 14.7 6.7 ± 6.7

Month 2 81.7 ± 9.0 5.0 ± 3.4

Month 4 90.0 ± 5.7 40.0 ± 8.1?

Month 6 83.3 ± 4.2 1.7 ± 1.7

Month 8 93.3 ± 3.3 15.0 ± 6.1

% Emergence-Confused Flour Beetle

Field Applications Data • Bartlett Milling reports a 90% reduction in returns the first Year.

• Mars Food reports a 100% elimination of returns due to insect damage in

2011 for corrugated bulk shipments. Normally 4-5 truckloads are returned annually.

• Southern States reports no incidents of insect damage in packaged seed products for 2011.

• Pioneer Seed is reporting good control with multiwall bagged Seed Corn

ProvisionGardTM Test Results

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Shipper Number No Column represents Zero Insects

ProvisionGard Pet Food Multiwall -

Treated Control

90 Day Field Study- SOS Bag

Pet Food SOS Bags Fort Worth Texas Distribution Center Control Treated Total # of Shippers 41 41 Total # of Insects 58 3 Average # of insects/shipper 1.4 0.07 Range of insect count/shipper 2 – 16 0 – 1

Average Reduction in Treated vs. Control 95% efficacy

ProvisionGardTM Test Results

Learnings from Testing Results Infestation typically reduced 85-99% Works on both exterior & buried substrate Effective on a broad spectrum of insects Results are consistent between lab and field