combating multidrug resistance bacteria with essential oils
TRANSCRIPT
COMBATING MULTIDRUG RESISTANT BACTERIA WITH ESSENTIAL OILSMiguel Diaz
MICROBIAL COMPETITION
http://www.livescience.com/
• Competition for limited resources• Excretion of chemicals that are
toxic and inhibit growth
http://virtuallaboratory.colorado.edu
COEVOLUTION• Intrinsic resistance• Spontaneous mutations
http://learn.genetics.utah.edu/
PATHOGENS• Disease causing organisms
www.cdc.gov
http://www.bacteriainphotos.com/bacteria-photo-gallery.html
BACTERIA• Bacterial
Classification• Gram Positive• Gram Negative
DISCOVERY OF ANTIBIOTICS• 1928 Alexander Fleming
discovered Penicillin• Antibiotics have had a
significant impact on human health and life
• “Wonder” drug
http://sciencenational.tumblr.com/
MODES OF ACTION• Selective for bacterial cells• Unique processes• Metabolic pathways
• Bacteriostatic vs Bactericidal• Narrow vs Broad Spectrum
ANTIBIOTICS• Treat bacterial infections• Life saving• extending the human lifespan
• Naturally, Synthetic, Semi-Synthetic• Zone of inhibition• Minimum Inhibition Concentration
(MIC)
ERA OF ANTIBIOTICS
• Overuse• Misuse
"The thoughtless person playing with penicillin treatment is morally responsible for the death of the man who succumbs to infection with the penicillin-resistant organism.”
A. Fleming
RESISTANCE• Intrinsic Evolutionary event• Genes chromosomal or plasmid
• HGT• R plasmids
https://www.niaid.nih.gov
R-PLASMIDS
https://classconnection.s3.amazonaws.com
• Plasmids• Non-chromosomal DNA• Housekeeping genes• Some carry resistant genes• R-plasmids
ANTIBIOTIC RESISTANCE
MECHANISM OF RESISTANCE• Modify and inactivate
• ẞ-lactams • Penicillin
• Prevent drug uptake• Chloramphenicol
• Pump it out• Efflux Pump
• Tetracycline
HORIZONTAL GENE TRANSFER• Sharing of genetic material
• across different genera• 3 mechanisms
• Transformation• uptake of DNA from
the environment• Transduction
• Virus mediated• Conjugation
• cell to cell sharing • type IV pili
MULTIDRUG RESISTANCE
http://www.moleculin.com/
Multidrug Efflux Pump
http://evolution.berkeley.edu/
• R-plasmid with many resistant genes
MULTIDRUG RESISTANCE• ESKAPE Pathogens• Enterococcus faecium (VRE)• Staphylococcus aureus (MRSA)• Klebsiella and Escherichia coli producing ESBL• Acinetobacter baumannii• Pseudomonas aeruginosa• Enterbacteriaceae
POST ANTIBIOTIC ERA?
• Little research and development• Mutations can happen quickly
and are easily shared• What now?
“ We are headed to a post antibiotic era, in which common infections and minor injuries can once again kill” WHO 2015
http://bk.asia-city.com
ESSENTIAL OILS
http://www.questnetproxy.com/
• Traditional medicine• Topical or inhaled• Some toxic if ingested
• Generally safe• Chemotype• Changes in chemical
phenotype• Steam distillation
ESSENTIAL OIL: MODES OF ACTION
http://www.mdpi.com/pharmaceuticals
• Degrade cell wall• Damage cytoplasmic membrane• Increase permeability• Leakage of cell contents• Damage membrane proteins• Coagulation of cytoplasm• Depletion of proton motor force
TERPENOIDS• Secondary plant metabolite• Isoprene subunits• Volatile Compounds• Easley evaporated• Give plants fragrance (essence)
MELALEUCA ALTERNIFOLIA
http://www.teamessence.com.my/
• Native to Australia• Aboriginals use it for treating
infections• Know for its antiseptic, antibiotic
properties• Main constituents
• terpinen-4-ol • -terpineol
TEA TREE OIL• Cell Membrane Permeability• Leak K+
• E. coli and S. aureus• Cell Respiration• Decrease O2 consumption rate
TEA TREE
Lee et al. 2014
MENTHA PIPERITA
https://www.tuinplant.nl
• Native to Europe and Middle East
• Menthol and Mentone• Medicinal Uses• Analgesic, cooling,
antiemetic• muscle rubs
Bakkali et al. 2008
PEPPERMINT OIL
Singh et al. (2011)
PEPPERMINT OIL
Bazargani and Rohloff (2016)• Anti-plasmid activity• Menthol 96%• In combo with promethazine 100%
Anti-biofilm
EUCALYPTUS CAMALDULENSIS
https://upload.wikimedia.org/wikipedia/commons/8/82/Eucalyptus_trunk_zebra_pattern.jpg
• Indigenous to Australia• Medicinal Purpose
• anesthetic, antiseptic, astringent• Constitutes
• p-cymene (35.0%)• cryptone (13.7%)• tanins (11%)• terpinen-4-ol (5.7%)
EUCALYPTUS
http://www.mdpi.com/molecules
Jazani et al. 2012
OTHER ESSENTIAL OILS
ESSENTIAL OILS AND MDRO• Essential oils can reduce the use of antibiotic
• Preventative measures• Reduce inflammation response• Antiseptic
• Cleaning hospital surfaces• Alternative treatments
• Effective against a broad spectrum of bacteria• Synergistic effects
• In combination with antibiotics• Decrease MIC• Increase zone inhibition
REFRENCES• Bazargani MM, Rohloff J. 2016. Antibiofilm activity of essential oils and plant extracts against
Staphylococcus aureus and Escherichia coli biofilms. Food Control 61:156–164.• www.cdc.gov• Cox.S.D. 2000. The model of antimicrobial action of the essential oil of Melaleuca alternifolia. J. Appl.
Microbiol. 88:170–175.• Jazani NH, Mikaili P, Shayegh J, Haghighi N, Aghamohammadi N, Zartoshti M. 2012. The hydro-
alcoholic extract of leaves of Eucalyptus camaldulensis Dehnh. has antibacterial activity on multi-drug resistant bacteria isolates. J. Appl. Biol. Sci. 6:37–40.
• Knezevic P, Aleksic V, Simin N, Svircev E, Petrovic A, Mimica-Dukic N. 2016. Antimicrobial activity of Eucalyptus camaldulensis essential oils and their interactions with conventional antimicrobial agents against multi-drug resistant Acinetobacter baumannii. J. Ethnopharmacol. 178:125–136.
• Lee RLP, Leung PHM, Wong TKS. 2014. A randomized controlled trial of topical tea tree preparation for MRSA colonized wounds. Int. J. Nurs. Sci. 1:7–14.
• Singh R, Shushni MAM, Belkheir A. 2015. Antibacterial and antioxidant activities of Mentha piperita L. Arab. J. Chem. 8:322–328.