column category - gl sciences, inc€¦ · cu inertness to complexes oxine-copper durability of...
TRANSCRIPT
-
GL Sciences Inc.
Inertsil 3 Series
New Inertsil ODS Sprint Inertsil Ph-3New Inertsil ODS-3 4um New Inertsil Hilic
Inertsil ODS-3 Inertsil DiolInertsil ODS-3V Inertsil CN-3 Inertsil ODS-EP Inertsil NH2Inertsil ODS-P Inertsil AX
New Inertsil Sulpha C18 Inertsil CXInertsil C8-3 Inertsil SIL-100A
Column Category
-
GL Sciences Inc.
Inertsil 2 Series
Inertsil ODS-2Inertsil C8Inertsil C4Inertsil PhInertsil SIL-150A
Column Category
-
GL Sciences Inc.
Inertsil for Proteins and Peptides
Inertsil WP300-C18Inertsil WP300-C8Inertsil WP300-CInertsil WP300-DiolInertsil Peptides-C18Inertsil SIL-150A
Column Category
-
GL Sciences Inc.
Controlling FactorsBase Silica-Gel Chemical Bonding Selectivity of Packings Inertness of Packings Durability of Packings Column Performance
Inertsil Quality Control
-
GL Sciences Inc.
QC Tests for Lots
Inertsil Quality Control
Category Method DescriptionBase Silica-Gel SEM Sphericality and Surface Smoothness by Scanning Electron Microscopy.
Ph Particle Size Uniformity by Nitrogen Adsorption Laser Ray Particle Analyzer.M Metals by Atomic Emission and ICPPh Particle Size, Surface Area, Pore Diameter and Pore Volume. by Nitrogen Adsorption, Laser Ray Particle Analyzer
Chemical Bonding C Carbon Loading by Elementary AnalysisSi NMR Residual Silanol by Si-NMR Schro Residual Silanol by Chromatographic test
Selectivity of Packings T Hydrophobicity Tanaka Test MixtureT Stereoselectivity Tanaka Test MixtureT Hydrogen-bonding capability Tanaka Test Mixture
Inertness of Packings P Inertness to Basic compounds PyridineAm Inertness to Basic compounds Ethylaniline, Benzylamine, AminopyridineAc Inertness to Acidic compounds Formic Acid, Acetic AcidCu Inertness to Complexes Oxine-Copper
Durability of Packing D Durability to Low pH mobile phase at pH 2.0D Durability to High pH mobile phase at pH 9.0
-
GL Sciences Inc.
Inertsil Quality Control
Sphericality and Surface Smoothness by Scanning Electron Microscopy
-
GL Sciences Inc.
Inertsil Quality Control
Particle Size, Surface Area, Pore Diameter and Pore Volume are Controled by Using Nitrogen Adsorption and Laser Ray Particle Analyzer.
0.000
200.000
400.000
600.000
800.000
1000.000
1200.000
FSQ5-
138
FSQ5-
139
FSQ5-
140
FSQ5-
141
FSQ5-
142
FSQ5-
143
FSQ5-
144
FSQ5-
145
FSQ5-
146
FSQ5-
147
Pore
Volu
me &
Surf
ace A
rea
0.00
20.00
40.00
60.00
80.00
100.00
120.00
Pore
Siz
e
Part
icle
Siz
e
Surface Area (m2/g)
Pore Volume (uL/g)
Pore Size ()
Particle Size (m)
-
GL Sciences Inc.
Inertsil Quality Control
Packings with an Uniform Size and Smooth SurfaceShow Very Low Column Back Pressure
Column back pressure
0
50
100
150
200
250
0 20 40 60 80 100 120
Methanol concentration in H2O
e
PRODIGY 5 ODS-2 (5um) S/NO 143250
TSKgel ODS-80Ts QA (5um) C-No.W0004
YMC-Pack Pro C18 (5um) No.042527052(W)
J'sphere ODS-M80 (4um) No.042522712(W)
PRODIGY 5u ODS(3) 100A (5um) S/NO 145865
COSMOSIL 5C18-AR (5um) Manf.No.H11279
L-column ODS (5um) No.101097
ZORBAX SB-C18 (5um) Serial No.CL3106
Mightysil RP-18 (5um) Lot.No.960134
Inertsil ODS-3 (5um) TQ5-1219
-
GL Sciences Inc.
Inertsil Quality Control
High Base Silica-Gel PurityAmount of Metals Are Inpected by
Atomic Emission and ICP
-
GL Sciences Inc.
Inertsil Quality Control
High Base Silica-Gel Purity ( Amount of Metals Are Inpected by Atomic Emission and ICP)
-
GL Sciences Inc.
Inertsil Quality Control
Chemicaly Bonded Carbon Loading AreInspected by Elementary Analysis
-
GL Sciences Inc.
Inertsil Quality Control
Chemical Bonding - Residual Silanol Groups areinspected by 29Si-NMR
Silanol Si-OH
Slica gel structure Si-O-Si
Silanol Si-OH
-
GL Sciences Inc.
Inertsil Quality Control
Chemical Bonding - Residual Silanol Groups areinspected by 29Si-NMR
remaining silanol
ODS groups
C18H37- Si-O-
-
GL Sciences Inc.
Inertsil Quality Control
Chemical Bonding - Residual Silanol Groups areinspected by 29Si-NMR
End-capped groups
CH3)3- Si-O-
-
GL Sciences Inc.
Inertsil Quality Control
29Si-NMR spectra of Inertsil ODS-3 and commercially available ODS packing materials
Inertsil ODS-3Commercially available ODS column A
residual silanol
endcapping
residual silanol
endcappingendcapping
residual silanol
Commercially available ODS column B Commercially available ODS column C
End-capping groupCH3)3- Si-O-
-
GL Sciences Inc.
Inertsil Quality Control
-Residual Silanol Groups-by Chromatographic Tests (Pyridine/Phenol)
Column : 250x4.6mmI.D. Eluent:CH3OH/H2O=30/70Detection : UV at 254 nmColumn Temperature : 40Peak identification : 1) Pyridine 2) Phenol
0 2 4 6 8 10 12 14 16 18
Time (min)0 2 4 6 8 10 12 14 16 18
Time (min)0 2 4 6 8 10 12 14 16 18
Time (min)0 2 4 6 8 10 12 14 16 18
Time (min)
-
GL Sciences Inc.
Inertsil Quality Control
GL Sciences is using Prof. Tanakas selectivity test for QC
(n-amylbenzene) / ( n-butylbenzene) : Hydrophobicity, (CH2) (triphenylene) / ( o-terphenyl) : Stereo selectivity, (t/o) (caffeine ) / (phenol) : Hydrogen-bonding capacity, (c/p) According to Prof. Tanakas group: The (CH2) value is a better measure of the surface coverage than the carbon content. The (t/o) values are determined by the functionality of silylating
reagents and the surface coverage. The (c/p) divided by (CH2) illustrates the effect of hydrogen bonding by canceling the difference in the hydrophobic property.
-
GL Sciences Inc.
Inertsil Quality Control
Separation selectivity test for ODS columnsk (n-Amylbenzene) / k (n-Butylbenzene) : the hydrophobicity of the ODSk (Triphenylene) / k (o-Terphenyl) : the stereo-selectivity of the ODSk (Caffeine) / k (Phenol) : the quantity of unreacted silanol groups in the ODS
0 10 20Time (min)
1)Uracil
2) Caffeine
3) Phenol4) Butylbenzne5)o-Terphenyl6) Amylbenzene
7) Triphenylene H3C N
CH3
C
-
GL Sciences Inc.
Inertsil Quality Control
-Inertness to Basic Compounds-Pyridine/Phenol
-
GL Sciences Inc.
Inertsil Quality Control
-Inertness to Acidic Compounds-Formic acid/ Acetic acid
-
GL Sciences Inc.
Inertsil Quality Control
-Inertness to Chelating Compounds-Oxine Copper
-
GL Sciences Inc.
Inertsil Quality Control
-Durability to Acidic Condition -0.1% Trifluoroacetic acid in H2O pH2.0
-
GL Sciences Inc.
Inertsil Quality Control
-Durability to Alkaline Condition CH3OH/0.01M Na2HPO4(pH9)=40/60
-
GL Sciences Inc.
Inertsil Quality Control
-Column Performance Measurement-
CalucalationN : Number of theoreticalplatesRt : Retention timeW/2 : Half-height width
150x4.6mmI.D. 13,000 more250x4.6mmI.D. 22,000 more
S : peak asymmetry 5%peakheight
1leading,fronting1TailingPeak asymmetry 0.95
1.10
w/2
-
GL Sciences Inc.
Inertsil Quality Control
-
GL Sciences Inc.
Inertsil Quality Control
Slide Number 1Slide Number 2Slide Number 3Slide Number 4Slide Number 5 Inertsil Quality Control Inertsil Quality Control Inertsil Quality Control Inertsil Quality Control Inertsil Quality Control Inertsil Quality Control Inertsil Quality Control Inertsil Quality Control Inertsil Quality Control Inertsil Quality Control Inertsil Quality Control Inertsil Quality Control Inertsil Quality Control Inertsil Quality Control Inertsil Quality Control Inertsil Quality Control Inertsil Quality Control Inertsil Quality Control Inertsil Quality Control Inertsil Quality Control Inertsil Quality Control