collection of materials in bacterial and viral …
TRANSCRIPT
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COLLECTION OF MATERIALS INBACTERIAL AND VIRAL
DISEASESDISEASES
(Part II)
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HAEMORRHAGIC SEPTICAEMIAFrom sick animals-Smears from blood and throat
swelling.
From dead animals-Smears from heart blood & liver,heart blood in sterile containers,lymph nodes & liver,heart blood in sterile containers,lymph nodes & spleen on ice.
BLACK QUARTER
Impression smears from affected muscle,exudate from lesions,pieces of affected muscles on ice.
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ANTHRAX
Cattle and sheep-Flame fixed blood smears.
Horse,Swine & Dog-Smears from s/c swelling.
In dead animals- Swabs of blood from ear vein for cultural examination.
Ear/muzzle piece on ice.Ear/muzzle piece on ice.
GLANDERS
Exudate from skin and lung lesions on ice.
Impression smears from exudate.
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BRUCELLOSIS
Serum,semen uterine exudate on ice.
Heart blood and abomasal contents of
aborted fetus,placenta with cotyledons.
Vaginal swabs in PBS on ice.
LEPTOSPIROSIS
Blood,serum,milk & urine in sterial vials on
ice.
Pieces of liver and kidney in 10% formalin.
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TUBERCULOSIS
Live animal-cough material,milk sample in sterile
container.
Dead animals-tissue pieces from suspected lesion
in 10% formalin, smears from lesions,lymph glands
lung lesions in sterial containers in 50% buffered lung lesions in sterial containers in 50% buffered
glycerine.
JOHNE’S DISEASE
Rectal pinch smears,bowl washings.
Dead animals-terminal portion of ileum with ileo-caecal
valve, mesentric lymph gland in 10% formalin.
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ENTEROTOXAEMIA
Contents of small intestine with and without
chloroform separately, kidney pieces,urine
on ice.
ACTINOMYCOSIS & ACTINOBACILLOSIS
Smears from pus lesions,pus in vials on
ice,formaline preserved materials from
affected muscles.
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BOVINE GENITAL CAMPHYLOBACTERIOSIS
In males-
Preputialwashings/mucus/smegma/secretions smears.
In females-
Vaginal mucus/lavage,aborted fetus,placenta,stomach contents,lungs,liver etc. contents,lungs,liver etc.
SALMONELLOSIS
Intestinal swab,heart blood,bile, liver spleen in sterile containers on ice.
LISTERIOSIS
Aborted foetus,placenta,internal organs in sterile vials on ice & 10% formaline saline separately.
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MYCOPLASMOSIS/CCPP/CBPP/CORYZA
Swabs from lesions,nose & vagina in PBS on ice,paired serum & lung pieces in 10% formaline & on ice separately.
CONTAGIOUS EQUINE METRITIS
Urogenital swabs from mare and stallion and paired serum samples.
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COLLECTION OF MATERIALS IN VIRAL
DISEASES
PPREye, mouth and rectal swabs in PBS on ice,blood at the
height of temp. in anticog.,Pre-scapular lymph nodes,spleen,intestine on ice & lung,liver,spleen,tonsils etc. in 10% formaline.etc. in 10% formaline.
FOOT & MOUTH DISEASE
Curetted epithelium from tongue & foot lesions in 50% PBS
on ice. Pieces of heart & other organs in calves in 10% formal saline and ice separately.
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MAD COW (Bovine Spongiform Encephalopathy)
Tissue pieces from Medulla Oblongata and cerebellum in 10% formaline and on ice separately.
RABIES
Half portion of brain , salivary glands in 50% PBGS and
rest half in 10% formaline in leakproof hard box.
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GROSS STRUCTURE OF BRAINGROSS STRUCTURE OF BRAIN
CEREBRAL CORTEX
CEREBELLUM
CEREBRAL CORTEX
THALAMUS
HIPPOCAMPUS
MEDULLA OBLONGATA
HYPOTHALAMUS
OLFACTORY BULBS
Spinal cord
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Sample Collection and Preparation
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Sample for Histopathology
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Actual Photograph of Obex
Brainstem: the obex region
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SWINE FEVER
Blood at the height of temp. 20 ml. with anticoagulent on ice from live animal.
Heart blood, pieces of spleen,l.node,pancreas Heart blood, pieces of spleen,l.node,pancreas in 50%PBGS.
Pieces of brain,lung,intestines(ileo-caecal junction) and kidneys in
10% formal saline.
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BLUE TONGUE DISEASE
Blood at the height of temp.with anticoagulent,paired sera,spleen ,l.nodeson ice,spleen,l.nodes,intestine etc.in formaline.
CANINE DISTEMPERCANINE DISTEMPER
Impression smears of visceral organs ,pieces of lung ,u.bladder,liver,trachea & brain in formaline.
AFRICAN HORSE SICKNESS
Whole blood & serum,spleen,brain and lung in 50% PBGS and formaline separately.
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THANKS