Cosmetic Laser Solutions MedSpa 857-288-8257www.cosmeticlasersolutions.net

Collagen Booster Serum

Collagen Booster

®

Before After

Before

Before

After

After

Production of total collagen, collagens I and III (ex vivo tests)

The action of NF-13 Complex on total collagen production was evaluated on skin explants from a 74 years old donor. Skin explants were either

day 6 and day 11. Total collagen was detected with staining assessed by microscopical observation and image analysis.

Results: NF-13 Complex dramatically increases the vitality

of young and aged fibroblasts. Aged fibroblasts

are enabled to function property again.

Results: NF-13 Complex increases the synthesis of

Pro-collagen I in a dose dependent way.

Aged fibroblasts show even better ratio of

production compared to young fibroblasts.

Results: NF-13 Complex used at 0.5% increases the

quantity of total collagen by 6.8% in 11 days.

*p<0.01 compared to untreated, Student t-test

**p<0.001 compared to untreated, Student t-test

Biological activity

17 20

36

80

60

40

20

0

Improvement of fibroblasts vitality

Improvement of mean viability(% improvement

versus untreated cells)

0.08% 0.4% 2%

Young fibroblasts

Aged fibroblasts

50

40

30

20

10

0

*

**

Production of Pro-collagen I in vitro

Concentration of Pro-collagen I

in ng/ml(% improvement

versus untreated cells)

0.1% 0.5% % of NF-13 Complex2%

No treatment

NF-13 Complex0.5%

90

85

80

75

Total collagen

Percentage of surface occupied by total

collagen (%)

Day 1 Day 6 Day 11

+6.8%

®

NF-13 concentration0

25

50

75

100

MM

P en

zym

atic

act

ivity

(%

con

trol

)

Inhibition of MMP Enzymatic Activity Mean reduction in mild to moderate rosacea

from baseline at 12 weeks

MMP-9 MMP-2

Capillary

Clarity

Capillary

branching

Capillary

length

0

0.25

0.50

0.75

1.00

1.25

1.50

Impr

ovem

ent f

acto

r

a

b

c

Placebo

NF-13 Complex

NF-13 inhibits more than 80% of MMP enzymes activity

(Types 2 and 9). The MMP-12 enzyme is also inhibited by

NF-13.

23 volunteers (27 enrolled) with mild to moderate rosacea,

visible telangiectases.

Randomized split-face, double-blind trial, 12 weeks with a

5% NF-13 Complex cream or placebo.

a p<0.03 - b p<0.006 - c p<0.0008

untreated or treated with 0.5% of NF-13 Complex at day 0, day 1, day 4, day 6 and day 8. Explants were then sampled and analyzed at day 0,

After studying the total collagen, two different types of collagens were quantitatively evaluated by immunostaining: collagen I (in green

fluorescent), and collagen III (in dark rose), also known as the youth collagen.

Results: NF-13 Complex at 0.5% significantly increases collagen I and collagen III synthesis by +179% and +194% respectively after 11 days,

versus untreated. Upon aging, the ratio of collagen I / collagen III is increased. With Neodermyl® at 0.5%, this ratio is decreased to the level of

a younger skin: it moves from 0.82 to 0.55.

Production of elastin (ex vivo tests)The action of NF-13 Complex on tropoelastin and elastin production was evaluated by following the same protocol as for collagen I and III.

Tropoelastin (in green fluorescent) and elastin (in green fluorescent) were detected by immunostaining assessed by microscopical observation

and image analysis.

Results: NF-13 Complex significantly increases tropoelastin and elastin production by +57% and +190% respectively after 11 days, versus untreated.

Untreated – Day 11 Untreated – Day 11NF-13 Complex 0.5% – Day 11 NF-13 Complex 0.5% – Day 11

Untreated – Day 0

NF-13 Complex 0.5%Day 11

Untreated – Day 0

NF-13 Complex 0.5%Day 11

*p<0.01 compared to untreated, Student t-test *p<0.01 compared to untreated, Student t-test

Biological activity

No treatment No treatmentNF-13 Complex NF-13 Complex

40

30

20

10

0

40

30

20

10

0

Collagen type I Collagen type III (Youth Collagen)

Percentage

of surface

occupied

by collagen

I (%)

Percentage

of surface

occupied

by collagen

III (%)

Day 0 Day 0Day 6 Day 6Day 11 Day 11

+179%*+194%*

*p<0.1 compared to untreated, Student t-test **p<0.05 compared to untreated, Student t-test

No treatment No treatmentNF-13 Complex 0.5% NF-13 Complex 0.5%

14

12

10

8

6

4

2

0

1816141210

86420

Tropoelastin Elastin

Percentage

of surface

occupied by

tropoelastin

(%)

Percentage

of surface

occupied by

elastin (%)

Day 0 Day 0Day 6 Day 6Day 11 Day 11

+57%*+190%**

®

Fast improvement of firmness and elasticity (clinical)

The two parameters R0 and R5 were measured by Cutometry to evaluate the performance of NF-13 Complex at 1% on firmness and elasticity.

Results: Using NF-13 Complex at 1% increases firmness by 13 times and elasticity by 1.6 times in only 15 days. These improvements of skin

parameters are sustained after two months of treatment.

*p<0.01 compared to placebo, Student t-test

**p<0.1 compared to placebo, Student t-test

*p<0.01 compared to T0, Student t-test

Efficacy

Placebo NF-13 Complex 1% Placebo NF-13 Complex 1%

6

4

2

0

-2

-4

-6

-8

-10

14

12

10

8

6

4

2

0

Firmness (R0) Elasticity (R5)

Improvement

of skin

firmness (R0)

(% versus T0)

Improvement

of skin

elasticity (R5)

(% versus T0)

Day 15 Day 15Day 60 Day 60

x13* x12**

+60%*

*

+44%

Wrinkles’ profiles confirm the benefits of using NF-13 Complex at 1%. After 15 days, the results are outstanding:

Results: The skin is smooth and wrinkles are visibly reduced after only 15 days of treatment with NF-13 Complex use at 1%.

NF-13 Complex delivers equivalent results to one injection of collagen filler in just 2 weeks: -15% of wrinkles reduction

(source American Society of Plastic Surgeons – 2013).

Wrinkle profile – Untreated – Day 0

Volunteer number 13 - 67 years old - crow's feet area

Wrinkle profile – NF-13 Complex 1% – Day 15

®