cm0463, standard plate count agar apha _ oxoid - product detail

1/3/14 CM0463, Standard Plate Count Agar APHA | Oxoid - Product Detail www.oxoid.com/UK/blue/prod_detail/prod_detail.asp?pr=CM0463&c=UK&lang=EN&org=&print=Y&sec=1 1/2 You are viewing the printer friendly version of this page. To return to the regular view click here. Dehydrated Culture Media Sector: Industrial STANDARD PLATE COUNT AGAR (APHA) Code: CM0463 A standard medium corresponding to the APHA formulation for milk, water, food and dairy products. Typical Formula* gm/litre Yeast extract 2.5 Pancreatic digest of casein 5.0 Glucose 1.0 Agar 15.0 pH 7.0 ± 0.2 @ 25°C * Adjusted as required to meet performance standards Directions Suspend 23.5g in 1 litre of distilled water. Bring to the boil to dissolve completely. Dispense into bottles and sterilise by autoclaving at 121°C for 15 minutes. Description Standard Plate Count Agar was developed by Buchbinder et al. 1 who wished to use an agar without milk solids in the formulation and investigated the control tests necessary to give standard results in dairy products with statistically valid counts. Standard Plate Count Agar meets the formulation of the APHA 2 , AOAC 3 and PHLS 4 . Storage conditions and Shelf life Store the dehydrated medium at 10-30°C and use before the expiry date on the label. Store the prepared plates at 2-8°C. Appearance Dehydrated medium: Straw coloured, free-flowing powder Prepared medium: Straw coloured gel Quality control Positive control: Expected results Escherichia coli ATCC® 25922 * Good growth; straw coloured colonies Negative control: Uninoculated medium No change * This organism is available as a Culti-Loop® Precautions When carrying out prescribed Standard Methods it is essential to follow the protocols given in precise detail. References 1. Buchbinder et al. (1951) Public Health Reports. 66. 327-329. 2. American Public Health Association (1976) Compendium of Methods for the Microbiological Examination of Foods. APHA Inc. Washington DC. 3. Association of Official Analytical Chemists (1995) Bacteriological Analytical Manual. 8th Edn. AOAC. Washington DC.

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Page 1: CM0463, Standard Plate Count Agar APHA _ Oxoid - Product Detail

1/3/14 CM0463, Standard Plate Count Agar APHA | Oxoid - Product Detail

www.oxoid.com/UK/blue/prod_detail/prod_detail.asp?pr=CM0463&c=UK&lang=EN&org=&print=Y&sec=1 1/2

You are viewing the printer friendly version of this page. To return to the regular view click here.

Dehydrated Culture MediaSector: Industrial

STANDARDPLATE

COUNT AGAR (APHA)

Code: CM0463

A standard medium corresponding to the APHA formulation for milk , water, food and dairy products.

Typical Formula*

gm/litre

Yeast extract

2.5

Pancreatic digest of casein

5.0

Glucose

1.0

Agar

15.0

pH 7.0 ± 0.2 @ 25°C

* Adjusted as required to meet performance standards

DirectionsSuspend 23.5g in 1 litre of distilled water. Bring to the boil to dissolve completely. Dispense intobottles and sterilise by autoclaving at 121°C for 15 minutes.

Description

Standard Plate Count Agar was developed by Buchbinder et al.1 who wished to use an agar withoutmilk solids in the formulation and investigated the control tests necessary to give standard results indairy products with statistically valid counts.

Standard Plate Count Agar meets the formulation of the APHA2, AOAC3 and PHLS4 .

Storage conditions and Shelf lifeStore the dehydrated medium at 10-30°C and use before the expiry date on the label.Store the prepared plates at 2-8°C.

AppearanceDehydrated medium: Straw coloured, free-flowing powderPrepared medium: Straw coloured gel

Quality control

Positive control: Expected results

Escherichia coli ATCC® 25922 * Good growth; straw coloured colonies

Negative control:

Uninoculated medium No change

* This organism is available as a Culti-Loop®

PrecautionsWhen carrying out prescribed Standard Methods it is essential to follow the protocols given inprecise detail.

References1. Buchbinder et al. (1951) Public Health Reports. 66. 327-329.2. American Public Health Association (1976) Compendium of Methods for the MicrobiologicalExamination of Foods. APHA Inc. Washington DC.3. Association of Official Analytical Chemists (1995) Bacteriological Analytical Manual. 8th Edn.AOAC. Washington DC.

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