cls rapp10 eng web
TRANSCRIPT
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CONTENTS
NotefomtheCEO
2010Hghghts
CEllECTiSiNbriE
Oucoectvty
CEllECTiSiN2010
Ouscence
busnesseveopment
Copoteeveopment
Humnresouces-Communctons
SbSidiariES
Ceectsoesech
Ceectspntscences
Ectyce
Ceectsthepeutcs
iNaNCialSTaTEENTS
aPPENdiCES
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4 l Ceectsl actvtyrepot2010 l 5
Gowthneveopment
Wemesevecqustonsn2010,offengusthepomseofnewtechnoogc
nnovtonsnoostngounteectupopetypotfoo.inSepteme,foexmpe,
wepuchsethessetsofCytoPuse,compnythtspeczesneectopoton
systems,technquethtweneustontoucemegnuceses,oothemoecues,
ntowengeofffeentcetypes.Wesoecetopusuetheptneshpsunche
yCytoPuse,menngthtweenpostontoenetfomtheopetngcenses
gntefoeectopotontechnoogy.Onestnofoueveopmentsttegysseon
thecqustonoftechnoogesthtcompementougenomeengneengtechnoogesncneusefoongeofppctons,fomesechtothepy.inthepusutof
thsm,wehvesocquenon-excusvecenseghtsthtoffeusccesstonuce
pupotentstem(PS)cetechnoogyesutngfomthewokofKyotonvestysPofesso
ShnyYmnk.Ceectssthestcompnynthewotosecuecensesfohumn
thepeutcopophyctcppctonsunethspotfoonwemkngthese
technoogesvetotssusyEctycesothttcnecomeenggopye
nthse.
innovtonnpefomnce
Wehvemeconseepogessnoukeyusnesssectosthsye,othntemsof
scencenpoucteveopmentnntheeofnteectupopetynsesctvty.
itgoeswthoutsyngthttheesstottoeone,nwehvenotyetcheve
ougos.Howeve,weemoeconvncethnevethtwepossessneffectveeconomc
moentechnoogeswththepotenttomkecmpct.Ousuccesssune-
pnneythepeopewhomkeupougoup,ouesouces,oucutueofexceencen
ouvsonoftheotechnoogynusty,whchtogethehvemetpossefoustou
goupongosce.
d.anChouk, Chie Executive Ofcer
Weevngnnethtseenngtheveyessenceofotechnoogy.Thecoeconcept
ofotechnoogysseonusngvngognsmstotnsfompouctsymensof
nustmethos.Thsnotonwsevoutonzenthete1970swththescoveyof
ecomnntdNanthepouctonofthepeutcpotens,eveopmentsmeposse
youtytomofydNafgmentsntesttuesnentoucethepouctsofths
engneengpocessntoces,theeychngngthegenetcmkeup.Wehvenoweche
newstgenthsotechnoogcevouton.Chngngthegenetcmkeupofcessno
ongeowntochnceutspefomeusngtonmethos.ascpneoncechcteze
ygenetcmnputonsesutngfommkeshfttnkengwthvngognsmshsnowecomenchofengneengscence.dNapogmmngnowusespefect
knowegeoftheentegenomesequencetootnpecte,eenexctesut.
asentevouton
Pectonsntheeofotechnoogyeoftenoptmstc.butthespeetwhch
scentstshveeenetoecoethedNaofthehumngenome,nthedNaof
vngspeces,hsconstntysupssepectons.Toyweetthecuspofnew
enwhchgenetcnfomtonwevetoeveyone.itsestmtethty2013,
thecostofecongthehumngenomeweessthn1,000euospepeson,
nthepocesswepossenunefouweeks.injustfewyes,monsofpeope
whvethegenomeecoeneetousettoesgncustomzemec
tetments.Genomeengneengsstuteownstemofthswve.Thetechnooges
eveopeyCeectswmketpossetotetnnteocquegenomcefects.
Genomeengneengwgenetehostofnew,nnovtvepoucts,tkngupthe
tochfomfossnchemcesouces.Thesenewesoucesfomvngognsmsoffe
vstpotentfogowthnsustneeveopmentovethecomngeces.
leengenomeengneeng
Ceectssnstongpostonnthsemegngnustsecto.Weeveopnewcssesofthepeutcpouctswththepotenttotnsfomthewynwhchsesese
tetentocuesesesthtecuentyncue.Wesoeveoppoucts
foesech,ophmceutcpoucton,gootechnoogy,nucestemcesn,
moeecenty,ntheeoftentvefues.Ouppochtootechnoogyspose
tongfunmentchngestothsnustynthecomngecesntooffen
enesssouceofnnovtonngowthfoousocety.
NOTErOTHECEO
2010yeofconstucton
l Ceectsl actvtyrepot2010 l4
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2010HiGHliGHTS
CeectsntheenchNtoninsttute
foagcuturesech(iNra)sgn
coopetongeement
CellectsniNrawshetocollbote
ntheelsofcellbologynplnt
nnmlbotechnologes.They
ecetopooltheexpetse,whee
possble,tocquenewscentc
knowlege,tocetetechnologcl
nnovtons,ntoshethese
esults,knowlege,ntnsfetechnologytobusnesses,ptcully
tosmllnmeum-szeentepses
nthegofoo,gcultuen
envonmentsectos.
CeectsecevesiNPiNton
innovtonaw2009
CeectswonNtoninnovton
awfomtheenchNtoninsttute
foinustPopety(iNPi).Ceects
iPngechePqueeceve
thewfomthePesentofthe
iNPibo,Theyon.Thews,
ognzennuyytheiNPisnce
1991,honosmnmeum-sze
entepseswhosegowthcnett-utetotheoutstnngnnovton
pocesnwhchusecompehensve
nteectupopetysttegytoevege
usnessgowth. Cellectis represents a
fne balance between research activities
and a healthy management strategy,
sTheyon.
CeectsunchesCeectspnt
scences,susyecteto
pntppctons
Themnmsofthssusyeto
ncesencceeteusgeofCeects
popetytechnoogyngcutu
oogy,oenthecompnyssks
setottctnewnexpne
censngoppotuntesnexpoethe
eveopmentofpopetyttsfo
seecteppctons.Ceectspntscencessoctennnepos,
nnesot,Sa,wthotoyspce
cosetothenvestyofnnesot
ntsgeenhousefctes.itsChef
ScentcOfce,PofessodneVoyts,
cuentydectoofthenvesty
ofnnesotCentefoGenome
Engneeng,snntentony
enownescentstnestshe
expetnpntgenomeengneeng.
Newjounpuctonets in vvo
ctvtyofCeectsmegnucesesn
muscees
resechestthelvnvesty
HosptCentenQueec,Cn,
usemegnucesesengneeey
Ceectstoestoetheexpessonof
mcoystophnnhumnmyosts
in vitronnmuscees in vivo.
Theyweetheefoeetoshowthepotentofusngmegnucesesto
tetduchennemuscuystophy,
heetysesethtschcteze
ypegenetonofthemusce
tssue,eventuyengtoossof
motyneth.Theesutsofths
esechweepushenthescentc
jounGene Therapy.
Ceectsoesechnlonz
enteeveopmentncommec
mnufctunggeement
Ceectsoesechnlonz,wo
eenotechnoogymnufctue
hveenteentocontctfothe
eveopmentnmketngofoeng-
neeecenes.Ceectsoesech
wusetsmegnucesestonctvte
(knock-out)thegutmnesynthetse
(GS)nCHOK1SV,lonzspopetyhostcene.Johnbch,CSOof
lonzbophmceutcs,s,Lonza
is delighted to work with Cellectis
bioresearch. This collaboration makes
sense and will enhance our long-
standing and successful GS technology.
Newstuyshowsmegnucese-ven
tgetentegtonusngCeects
oesechscGPSCHO-K1kttoe
hghyefcentfougscovey
Scentcesechseonunque
methofogenetngstecenes
comptewthhghthoughput
sceenng(HTS)wspushenthe
Journal o Biomolecular Screening.
Thsesechshowethtthetechno-ogyeveopeyCeectsoesech,
seonmegnucese-ventgete
ntegton,sfste,moepctc
nmoeefcentthntton
methosnevngce-sessys
foHTSstues.
Jnury
Jnury
rc
h
apri
l
June
July
6
Styzemegnucese.
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Ceectscquesssetsfom
CytoPuseScencesinc.
CytoPulsespeclzesntheevelopment,
mnufctuenmketngofelecto-
potontechnologynequpment.
Electopotonshghlyefcentwy
fomoleculessuchsmegnucleses
tobentoucentonytypeofcell,
wthpplctonsngngfomesech
nbomnufctungtogcultue
nthepeutcs. CytoPulses assetsare a great strategic acquisition for
Cellectis,sCellectsChefExecutve
OfceanChoulk.
As we announced in 2009, part o
our development strategy is to acquire
technologies that complement our
existing meganuclease platorm and can
be leveraged across all aspects o our
business, rom research to therapeutics.
We saw such an opportunity in the
technologies at CytoPulse Sciences
Inc. and look orward to unlocking the
value o these assets.
Ceectsoesechnnounces
vtyofesechktsthoughnew
onneonne-stoe,povesenhnce
ccesstytomegnucesetechnoogy
Ceectsoesechnnouncethe
unchoftse-stoe,wthppoxmtey
onehuneey-to-usepoucts
vefoseonnevnewfunc-
tonnntutvewestetomeetthe
neesofothcemcnnusty
esech.The launch of a website withonline purchasing capability was a vital
step in improving researchers access
to our meganuclease technology,
expnelucSeg,VPSesn
ketngtCeectsoesech.
Our product oering has expanded
rapidly, and we now market over
one hundred dierent products on
three target markets.
Ceectsunchestsnewcopote
weste-infomtonshng,ntectvty
nnewmespce
Vstostothenewwesteeensue
enhnceustynnfomton
shng,ncungmoestemne
ccesstonfomtonoutCeects
technoogynpoucts.
ThenewCeectswestefocuseson
theecentthemes:esechntechnoogy,usnesseveopmentn
nvestoetons.
Ceectsoesechnnounces
estshmentofsusyntheS
loctettheCmgeinnovton
CentenCmge,sschusetts,
Ceectsoesechinc.weesponse
fotheS-wepomotonofCeects
oesechpouctsnsevces.
Ceectsn-censesnucepupotent
stemcetechnoogyfomPS
acemJpnstptentcense
nthethepeutce
Ceectsenteentotwoseptenon-
excusvecensegeementsgntng
thecompnywoweccesstothe
nucePupotentStem(PS)ceptent
potfoosngfomthewokof
PofessoShnyYmnkttheCente
foPSCeresechnappcton(Cra)tthenvestyofKyoto,Jpn.
Ceectssthestcompnynthewo
toecenseyPSacemJpnto
usethsptentpotfoofoppctons
nhumnthepeutcsnpophyctcs.
intheesechtooe,thssthe
stgeementwthenchcompny,
foownggeementswthSn
Gemncompnes.
CeectsgntsHvboscence
censetoCytoPuseeectopoton
nstuments
CeectsnHvapptus,vson
ofHvboscenceinc.whcheveops,
mnufctuesnmketso
ngeoftoostovncefescence
esechnegenetvemecne
wowe,sgnecensegeement
thtgntsHvapptusthe
woweexcusveghttomnu-fctuenmket,foesechuse,
thefuneofeectopoton-se
nstumentscqueyCeects
fomCytoPusenSepteme2010.
Ceects,howeve,etnsghtsto
theuseofthesenstumentsfotsown
esechneveopmentpogmss
wesncnctsnpophyctc
othepeutcpoceues,footh
humnsnnms.
Septem
ber
Septem
ber
Septem
ber
Octo
ber
Octo
ber
decem
ber
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CEllECTiSiNbriE
Thecompnyesgnsnmketsnuceses
odNascssossuchsmegnuceses,
nnovtvetooswhchenepecse
moctonofthegenome,swes
othepouctssngfomthstechnoogy.
Ceectsmstoexpotthefu potent
oftstechnoogynmecnenothe
esofscentconustexpoton.
Sncetsnceptonn1999yanChouk,
Phd,ndvSouve,Phd,Ceectshs
enjoyeexcusveghtstoptentpotfoo
owneytheinsttutPsteu.Pofessoroney
rothsten(Coumnvesty)chsts
Scentcavsoybo,whchcompses
ntentonyenownescentsts.
Tote,Ceectshsenteentomoethn
50geementswthphmceutco-
toes,seepoucesnotechcompnes
wowe,nhsfomemoethn20
cemcesechptneshps.
Snce2007,Ceectshseensteonthe
NYSE-EuonextatenextmketnPsn
hssecueove70monncptsnce
tsncepton.Whemntnngtsmtous
gowthtgets,Ceectshsveyso
cshnceuetonceseevenuefom
pouctses,megnucesepoucton,
ptneshpsncensegeements.
Wth10yesofwokonmegnuceses,
Ceectshscqueunmtcheexpetsen
theeofgenomeengneeng.Thenten
r&dnmegnuceseptfomtemshve
oknowegeoftheenthesks
equetoseectnntegtenewtech-
noogesthtwoupovecompementyto
Ceectsoffeng.
CEllECTiSiNbriE
Worldwide pioneer in genome engineering
Estshen1999,steontheNYSE-
EuonextatenextmketnPssnce
2007(alClS)
2010 operating revenues: 15,8
Staff: 129ncung51Phds
4 subsidiaries: Ceectsoesech,
Ceectsthepeutcs,Ceectspntscences,Ectyce
Applications: esechnomnu-
fctung,humnheth,gcutu
otechnoogy,stemces
Main technologies: genomeengneeng,
potenengneeng(nptcunuceses
odNascssos)
IP portfolio: 83ptentsgntenmoe
thn260ptentppctonspenng
Locations :Ps(nce);SntPu,
nnesotnCmge,sschussetts
(Sa)
As o December 31, 2010
Ceectsmpovesfeyppyngtsgenomeengneengexpetsetoongeofppctons,ncunghumnheth,oesechngcutue.
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egnucesesentupotensfounnmnysnge-ceeognsms.Theyehghy
speccdNascssos.Theyeetoecognzethenngsteyentfyngnucec
csequence(nuceccsetheconsttuentsofdNa),whchcontnsetween12n
ove30seps.Thsstessttstcyunquengenome.OncethedNaekhseen
me,thecectvtestsmntenncenepsystem(foexmpe,homoogousecom-
nton).ThssystemcoectsthedNamoecueyusng,foexmpe,tstwncopy(gene
coecton)osmfgmentspeccyntoucentothecesmoe(nsetono
knock-n).itsthesmepncpesthecopynpstefunctonnwopocessng.
Whengenejustneestoenctvtethethnepce,theokendNasounn
osesnfomton,theefoethetgetegenesnctvte(knownsknock-out).
Ceectsmnufctuesmegnucesesthtcncutpecsenewsequences,nethusto-
mefogenesofnteest.Thefestyofmegnuceseengneengowstheceton
ofwengeofspecctoostomofythetgetegenes.itsthetechnoogcvson
uponwhchCeectswsfoune11yesgo.Thswschevewhenthemegnucese
ptfomwsetopoucecustommegnucesespeccofnynewgeneofnteest,
wththesmechctestcssthewtypeones(efccy,speccty,).Thsexpetsen
genomeengneengtoospouctonhsoweCeectstoevovefomtoopouceto
pouct-evengcompny.
Genetcshsemonsttethelnkbetweenthephysclttbutesofspecesnthe
genes,ntheebyshownhowptculgenesemplctencetnsesesott-
butes.Genomeengneengenblesspecesgenestobemoenoetochngecetn
ttbutes,toectfyneo,otonewttofphysologcloeconomcnteest.
Thsppochsnotnew:coss-beengffeentplntsoselectngthebestnmlsfoepouctonsbseonthesmepncple.Themstompovespecesbygvngthem
thebestpossblettbutesoughttolenceopestesstncefocops,foexmple.
Thetgeteppochofgenomeengneengspecte,moeeenmoeeffectve
thneetechnques,ncneveneppetohumns.Thegenomesofove1,000
ognsmshvenoweenecoe.Knowegeofthesequencenoctonofthegenesof
vngengsstheefoeconstntympovng,eventhoughthewokngsofthesegenese
stnotfuyknown.Ouunestnngnthseowsustomnputegenesecty.
Theeetheepossesttegestooths:
insetonsusetonewttutetothegenome.oexmpenugscovey,
onoetoovecomegenetcefectkehemoph.
Coectonsusetoepcenexstngefectvesequence(whchgeneympctsthe
genesfunctons)yfunctonsequence.oexmpe,totetseousgenetcsese
suchsduchennemuscuystophy.
inctvtonsusetopeventtheexpessonofgene.Thsppochcneusetotet
pesstentvnfectonssuchsaidSohepes.
Outoo:egnucesesCompewtheetechnooges,theeffectvepoweofgenomeengneengesnts
tytonsetthegeneofnteestnthechosenpce.omnyyes,esechesmethos
weekntopyngtsnfoe.Theywoustuytheeffectofnomynsete
sequences,esptethefctthtnsetonnthewongpcecnhvehmfueffects:one
genecnensetentonothennctvtet,octvtecncemechnsms.Thss
wheeCeectshsthecompettveege.itsexpetsenmegnucesesmensthttse
topecseytgetspeccocusnthegenome.Ceectstoosgvescentststhepecson
theyweeckng.
Thepncpeofgenomeengneengssmpe:tnvovesmofyngthegenomeofnnvuospecesfothepuposesofunestnngthewytwoks,poucngusefupotensotetngsese.
egnucese
Chomosome
cut
OrCOrEaCTiViTY:GENOEENGiNEEriNG
Geneinseton
KNOCKiN GENErEPair
Geneinctvton
KNOCKOT
pastepaste
loss o a ewbase pairs
NonHomoogousEnJonng(NHEJ)
epmtxintegtonmtx
Homoogousrecomnton
Homoogousrecomnton
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OrCOrEaCTiViTY:aPPliCaTiONS
HumnhethGenomeengneengsthessfogenethepy.Oftencegenomesugey,ths
typeoftetmentcoueusetocuemnygenetcseses,suchssckecenem
(epnocytoss).resechnthsehseenceoutncootonwthCeectst
theemoSon-KettengCnceCentenNewYok.insem,theCNrS,na
(enchassoctongnstmuscuystophy)esocoseynvovenCeectspogess
nthethepeutce.butgenomeengneengcnsoeusetoghtvuseskehepes.
ThesepthogensnsetthedNantothedNaofnfecteces,menngthtthe
mmunesystemsunetogetofthempemnenty.Ceectstytotgetvey
pecseegonsofvusesgenomenoetonctvtethemmghtmketposse
toemovethegenetcmteofthevusesfounnthenfecteces.Thstheefoeepesentspotentekthoughnthetetmentoftheseseses.
agootechnoogyegnucesescneengneeesothttheyespecctonygeneofnyspecesof
pnt.Ceectsuseststechnoogynexpetsetopoveseepouceswthmeg-
nucesesthtcnmketgetemoctonsnpntgenomesntoeveopthenext
genetonofhghqutycoppnts.ToyCeectsseveopngptneshpgeements
wthsomeofthemjopyesnthegootechsecto,sothtgenomeengneengw
ecceptenweyusewthnthseofppcton.in2010,CeectsceteCeects
pntscences,susyectetogenomeengneengppctonsnpnts.itsm
stoncesencceeteusgeofCeectspopetytechnoogyngootechnoogy
ntoeveoppopetyttsfoseecteppctons.
resechnomnufctungGenomeengneengstthessofthemoenppochtofunmentesechn
oogy.Theentehumngenomesequencehseenente,swesthoseofmoe
thnthousnspeces.Howeve,oogstsonotknowtheenteoeofthesegenes
ththveeenente.Tonouttheoeofthesegenes,wecnusetgetedNa
moctontechnquestosuppessoegutetheexpessonofpecsedNasequence.resechescntheefoeunestntheoeofgeneystuyngtheeffectsoftssence
ovtonsntsexpessonotsstuctue.inomnufctung,megnuceseppctons
hveunquecompettvevntgesntemsofmnufctungpocesses,euctonncyce
tmenmpovementsnpotenquty.resechktsemketeyCeectsoesech
owngmegnucese-ventgetentegtonofgenentovouscenes.
TheymketesefoesechestomofythedNaofspecestheyestuyngn
fomnufctuestosceennevutethepouctsefcency.
resechtems
Xoot,dNapouctonnptesQpxoot,megnucesestesteontgets
Theptfomtemntsoots
Cooetestfomutntseecton
dNaunvestymkestpossefooutechnoogytoeppetonyknofspeces(humn,nm,pnt,cte,ov)ntoeveygeneofnteest.ThetechnoogyconcevenmsteeyCeectscneusetounockoestoethepotentofdNa,openngupongeofppctons.
OraCiliTiES
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OrGaNiZaTiON
CEllECTiSiN2010
oususesofCeectsweeceteetween2008n2010.Themstofuyeveoptheppctonsofoutechnoogywthnthegvenmketsectos.Thepentcompnycentzesthennceptmentofthegoup,thenteectupopety,theegeptmentnothesuppotfunctons,swestheesechneveopmentoungenomeengneengtechnooges.ThedNascssosptfompouctonwemnthesttegccenteofthecompnyswhoeswesthevngfoceofthegoupctvty.
Cellectis and its subsidiaries
Ectyce
EctycewsestshenSepteme2009toesechncommeczenust
usesofstemces.
Thentgosetoeveoptoosfogenetng
nucepupotentstemces
fomutces,oust,epouceffeenttonofstemces,nceesfotestngugcntes.
Ceectsoesech
ounen2008,Ceectsoesecheveopsn
mketsesechnpouctonktsthtmke
genomeengneengccessetooogc
esechesncompneswowe.
itoffestunkeysoutons,enngoogststoesgn
theywoktooswthcontonpecson,
suchsceneswththefetuesofhethy
oseseognotssue.
Ceectspntscences
Estshench2010,Ceectspntscences
ssusyectetotheppctonsof
megnucesestopnts.itsmnmssonstoncese
ncceeteusgeof
Ceectspopetytechnoogyngcutuoogy,oenthe
compnysptfomtottctnewnexpne
censngoppotuntesnexpoetheeveopment
ofpopetyttsfoseecteppctons.
Ceectsthepeutcs
Ceectsthepeutcs,unchen2008une
thenmeCeectsgenomesugey,sectetotheeveopmentofnnovtvethepeutcppochesusng
megnucesestotetgenetcseses,cncesn
pesstentvnfectons.Ceectsgenomesugeyseekstotetptents
suffengfomseoussesesesstnttoconventon
tetment.itspospectvethepytgetsthevey
dNasequenceesponsefothesesethtmye
ofcongentogn(genetcesese)ocque
(vnfectonocnce).
Ceects
Technoogeseveopment,potenpouctonptfom,nteectupopety,suppotfunctons.
17l Ceectsl actvtyrepot2010 l
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OrSCiENCE rESEarCHaNddEVElOPENT
d.cPques,ChefScentcOfce
Thschevementesutefompope
nustztonofoupocesses,whch
usttestheovemtutynpt-
tyoftheognzton.Thsenhnce
pouctoncpctymensthtCeects
cnpostontsefssuppeoftoos
foveyospectumofppctons
npoucts.
butthepogessththseenmen
oumnufctungpocessesshounotoveshowthevncesmenthe
ppctonofoupoucts.Oneofthe
mostsgncntchevementsstheuseof
oupouctsngenomesugey,whchs
nowhppenngonnunpeceentesce,
wthove15cootonswthcemc
esechsovethewo(HSr-TiGET
nn,Vibnleuven,ChenHospt
nboston,)poucngoth in vitron
in vivot.Thsepesentsgete
numeofmegnucesesuseswes
oevetyofppctonmethos,
ptcuythecetypesthtcne
successfuytgete.bothspectse
ectynketothencesenou
mnufctungcpcty,whchsmeetng
thegowngneesofoucustomes,
ouptnesnoun-houseesech
neveopmentctvtes.
in2010weuseozensofmegnucleses
nmmotlzecells,plnts,nmlmoels
nstemcells.Thsbopplcbltys
keyfetueofthepotentlofCellects
technology.Themountoftgthee
byffeenttems,bothtCellectsn
elsewhee,snctveoftheobustness
ofthstechnology.nyoftheseesultswll
bepublshethoughout2011.
ny,ouesechhseneustossessthechengesthtehe,ptcuy
n2011nntsthethepeutcsecto
whchhossomeoftheggestchenges
fous.amongthem,thetytogetou
moecuesnsufcentnumesntothe
tgetceswemjopotyfoou
esech.anothekeypotysensung
thesfetyofthsppoch,whchsecty
nketothespecctyofthemegnuceses.
Thespecctycteethemoststcty
ppenthepeutcppctons,
nsfetymoststngentyssesse.
Sfetynvectozton(othemetho
usetonsetmegnucesentotget
ce)etheefoethetwokeywos
fo2011,swespentheyefocusng
whoeheteyontheppctonsof
genomeengneeng cossesechtoos,gcutueomecne.
d.cPques
ThecoectvtyofCeectsntenr&dtemstoengneemegnucesesthtespecctochosendNatget,.e.tocetecustommegnucesesfoechgeneofnteest.
ThetemstCeectscnentfytheesesponsefothedNascsso
functonwthnthemegnucesestuctue,
swestheesthtcoesponto
theecogntonofspeccdNastes.Ou
esechesetheefoeetomofy
theecogntonndNaxtoneso
stogenetenewvntsthttgetnew
stesngenome.asesutofthsesech,
Ceectsnowhsgenkofpotens
wthove40,000motfsthtcne
comnetootnchmecpotens
cpeofnngtoffeentsequence
comntonsofthedNa.
inthelbstCellects,upstemesech
sspltntotwoes.Onetemof
esechesfocusesontheengneengof
potensmegnuclesesnptcul
noetompovethectvtynspec-
cty,ntoncesethesequencespce
thtcnbetgetebythesetlo-me
megnucleses.Theothetemfocuses
onthectvtyofmegnuclesesncells,
nptcullyonmpovngtheefcency
ofgenetgetngntsecombnton.Thstemslsowokngonnewppl-
ctonsfomegnucleses.
in2010,ther&dgoupmempotnt
pogess,ncungtheentctonof
newpotenstuctueswththecpty
toeengneeemoequckyn
offengnewpopetes.Thsetonew
megnuceseswthoptmzeusge.
aveycompetepucton,whch
escestheffeenttoosusen
genomeengneengncompesthe
popetes,wspushenCurrent Gene
TherapyytheCeectsesechtemt
theenof2010.
Meganucleases and other Tools for
Targeted Genome Engineering: Perspectives
and Challenges for Gene Therapy
SvG,Pootl,Gettor,SmthJ,
ontoyG,duchteuP,Pques.
CuGeneThe.2010,dec24
2010wsptcuypouctveyentemsoftheesechnvncementofoucoetechnoogy.Oneofoumnchevementswstheconsotonofoupouctonptfomnoetopouceeconumeofmegnuceses(271n2010).
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PrOdCTiONPlaTOr
Thescentstsentfythepotenmoues
whosedNatgetsmtchwthfgments
fomthetgetegenomeste.Thenthey
comnethemouestocetenew
megnucesewthecogntonspeccty
fotheequegenetget.Thectvty
ofechnewycetemegnuceses
thentestenseesoffunctonts
eveopeyCeects.
athecetonnnysswoksceoutythepouctonPtfomtem,who
cetefomstttonshtheenzymes
usensoyCeects.ThePtfom
temceteshghqutymegnuceses
compntwththepecsespecctonsof
echCeectsptneocustome,nten
nexten.ThePtfomtemcn
vtensecueoesysuppyngech
customewthsoutonthtestmeetsts
nees.sngtsyofoots,thePtfom
temsetomesuethectvtyof
vstnumeofmegnuceses.itsctuy
etounupto1montestspeweek.
Thesetethenpocesse,nyze
nusetoesgnmegnucesesthte
ppoptefoechpoject.ThePtfom
temenestheesecheptmentsto
ssessthetheoesnstnpouctoncontonswhefoowngstctpoceues
thtgunteefusmpetcetyn
quty.Thswokssuppoteyo-
nfomtcstemthtsuppesessentt
totheesechtems,enngthemto
pectthecoectstuctuesfoeveopng
effectvemegnucesesntheshotest
possetme.
bycyngouttsesechwok,the
Ptfomtemsetoexpnthe
tse,enhncngCeectsknowege
nscentcexpetse.2010wsmke
yeuctonnstztonn
mnufctungtmeswthnthePtfom.
Thsesutensgncntncesen
oupouctonoutput,wth271meg-
nucesesmnufctuen2010compe
wth99n2009.
atCeects,themssonofthecomputtonoogytemstopouceengneeemegnucesesusngtonppoch.
Newmegnucesesetoyessenty
pouceyso-cesem-ton
ppoch:coectonsofmegnucese
vntseutynomymofyngspeccesofthepoten,nthese
coectonsetesteytheptfomto
entfythemutntswththeexpecte
popetes.Stvngfotonppoch
mstetemnng,wthmoepecson,
whchvntsemoekeytoe
functonnoetogenetentest
smenumeofthesevnts.
TheCeectscomputtonoogypogm
mstompovePtfompefomncen
temsofevey,cost,cpcty,sequencng
enpouctquty.Theehseen
egupogessnthestfewyesn
wththeuseofnnovtvenewtechnques
ecentympementetCeects,wecn
etteessthecoepefomnce
mpovementmofcomputtonoogy.
Computtonesgnnvovesusng
softweseonenegyeveccutons
npoten,ncompeteyeesonthe
vtyofstuctut(.e.tmen-
sonstuctuesofmegnucesesoun
tothedNatget).Ceectsestshen
2009-2010ectecomputton
oogygoup,npewththesetup
ofmjostuctuoogypogm.
ThepogmshettheCNiO()
ncootonwthdGuemoontoy.Excusveccesstosgncntmountof
stuctuthseenkeyfctonthe
computtonoogygoupgnngette
unestnngofthemechnsmsgovenng
megnucese/dNantectons,n
optmzngthemoecumoengpocess
nguyepcngtheexpement
hgh-thoughputsceenngmetho.
antceescngthewokofCeects
onmegnucesesnthetechnques
usetocetenpoucenewpotens
thtcncutnewtgetswspushen
Novementhejoun Protein Engineering
Design and Selections.Thstceso
escestheongeofppctons
ofmegnuceses:eveopmentofce
nesenngpotenexpesson,moe
pntsnnms,thepeutcsppctons
kegenethepy,ocetonofnew
cssofntvugs.
The I-CreI meganuclease and its
engineered derivatives: applications from
cell modication to gene therapy
anouS,deenC,GzotS,
desseuxC,Pques,SvGH,SmthJ.
Protein Eng Des Sel. 2010,Nov3
Pnt:2011Jn.24(1-2)pp27-31
antcewspushenthescentc
jounNucleic Acids Research,esutng
fomcootonetweenCeectsn
d.Guemoontoysotoytthe
CNiOof.itescesthestuctueof
megnuceseountotsdNatget.
Molecular basis of engineered mega-
nuclease targeting of the endogenous
human RAG1 locus.uoziG,PetoJ,SumnnS,
CoomJ,reonoP,Vte,enoN,
enchno,damo,Gevsol,
GzotS,douss,SmthJ,Chon-Sotnei,
Pques,duchteuP,asa,Stche,
Senol,bncoJ,ontoyG.
Nucleic Acids Res. 2010,Sep16
Pnt:2011;39;729-743
21
COPTaTiONalbiOlOGY
d.GeogeSv,heofthecomputtonoogyeptmentWhenpojecteques
ffeentstenthegenometoemoe,CeectstemscetenewmegnucesefomtheOmegse,nkofmegnuceseswthmoechctestcs,popetyofCeects.
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bSiNESSdEVElOPENT
in2010,Ceectsenteentocensegeementwthboehngeingehemtootn,eenutzenmmoesfophmceutcesechthoughoutEuope,sweswthbyeHethCetousetshomoogousecomnton
ptents.baSpntscenceshssooenetscoo-tonwthCeectstoutzetscustommegnuceses.
acqustonofssetsfomCytoPuseinc.Eectopotonsveyefcentwyof
nsetngmoecuessuchsmegnuceses
ntonytypeofcenhsppctons
ngngfomesechnomnufctung
togcutuenthepeutcs.
Thoughthstnscton,Ceectscque
theeothetechnoogesseoneec-
topotonneveopeyCytoPuse:
demvxfotnsemdNavccnton,
Oncovetfovetenyvccntonpp-
ctons,nHymmunetomnufctuehyomsfomonoconntoes
poucton.Thetnsctonwsnze
onSepteme2,2010,fo$2.2.
HvapptusgntecensegeementThsgeementgnteHvapptus
thewoweexcusveghttomnufctue
nmket,foesechuse,thefuneof
eectopoton-senstumentstht
CeectscquefomCytoPuse.
Ceectsetnsghtstousethesenstu-
mentsfotsownesechneveopment
pogmsswesncnctsnpo-phyctcothepeutcpoceues,footh
humnsnnms.nethegeement,
Ceectswecevepymentof$1.3
fomHvapptus.Ceectswso
contnuetoecevennucensngfees
fomsomeoftscustomes.
lcensetouseEvogenuoescentpotensThsnon-excusvegeementwthEvogen
(oscow,russ)hsoweCeects
oesechtoncopotethesepotens
ntotsownpouctsnthusexpnts
ngeofgenomeengneengtoos.
Thentegtonmtxsoneofthekeycom-
ponentsoftheCeectsoesechkts.it
sthsmtxthtowstheusetoexpess
geneofnteest.byextenngthechoceofpomotesntgsnthesemtces,
Ceectsoesechcnoffetscustomes
fungeofmethostochevesnge-
phseeveopments,thussvngvue
tmeungexpements.
ageementwthlonzfomoctonofoengneeeceneCeectsoesechwusetsmeg-
nucesestonctvte(knock-out)thegutmnesynthetse(GS)genenCHOK1SV,
lonzspopetyhostcene.lonzs
woeenthepeutcpotenspouc-
ton.itstytocontopouctonofthe
hghestqutycenesmkesthecompny
pefectptnefotheppctonof
Ceectstechnoogyntsmegnuceses.
Weeeghtetoewok-ngwthCeectsoesech.Thscootonmkessensenwenhnceouong-stnngnsuccessfuGStechnoogy.
Johnbch,CSOoflonzbophmceutcs
lcensegeementwthwestOseesfopnttnsfomtonThsgeementenesCeectspnt
scencestousewestOseesaeoso
beminjecto(abi)technoogyfothe
tgetemoctonofpntgenomes.
ThewestOseesabitechnoogy
enesfunctonmegnucesestoe
eveeectyntopntces,thusvo-
ngtheneefodNavectos.Thsppoch
wowCeectspntscencestomofy
pntgenomeswthoutntegtngdNan
thepnt.
ThemofthebusnessdeveopmenttemstocommeczeCeectsnteectupopetyntocqueothecompnesiPthtwousuppottheeveopmentofnewtechnoogesopoucts.Ceectsnnovtventeectupopetyhsongeofppctonssuchsheth,gcutueoesech
egngstemcesntentveeneges.Thebusnessdeveopmenttemssonchgeoftheusnesscontctswthouptnes.
Thebusnessdeveopmentsttegy
compsesvemnes,whchw
hepCeectsvncetseeshp
ngenomeengneeng.
Thesee:
Togvepotytomegnucesesfo
thepeutcppctonsnoetotet
egenetcsesesnvnfectons,
ntoeveopnewpouctstoghtcnce
otompoveogenectnspntton
(tnspntetweentwogenetcy
ffeentpesons).
Topo-ctveytnsfetstechnoogyto
mjocemcnnustotoes
foesechpuposes.
Toententousnessgeementsnts
foumnesofppcton:hethce,
otechnoogy,gcutuotechnoogy,
nstemces.
Tostengthenpvegeptneshps
estshewthsomeofthemjopyes
ngcutuotechnoogy.
antosustnCeectscompettve
vntgeycontnungtoenhncets ptentpotfoonyncesngts
technoogcenexpetsenoe
tongvuetothepotfoo.
ombusnessdeveopmentpespectve,
2010ntteshftnfocusfom
outcensngofnteectupopety
towsgenetngncomefompoucts
eveopeusngsuchnteectupopety.
Wheesthemnsouceofevenue
emnecensestohomoogousecom-
ntonptents,thencopotonn
expnsonofCeectspntscences,
theexpnsonofthestemcectvtes
nEctycethnkstotheShnyYmnkPScense,nthecqustonof
theCytoPuseeectopotontechnoogy
wfomtheconestonesofpoucts
seonCeectsgenomeengneeng
technooges.Thstenwcontnue
though2011wheeCeectsexpects
sgncntncomefomcensngof
tsnteectupopetypotfoo,utso
expectstosgnnncesngnume
ofptneshpstoeveopnnovtve
pouctsnmutpees.
d.dkPoet,ChefbusnessOfce
d.dkPoet,ChefbusnessOfce
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ThemoftheCopotedeveopmenttemfo2010wstosetntoctontheoppotuntesvetousntheeofstemcesyestshngceptfomfopoputonengtotwoffeentppctons:esechtoosncethepy(tnspnts).
Themstoognzecoectonofces
thtepesentthephysoogy,pthoogy
nvestyofpoputonsothtwecn
unestnthsvesty in vitro.Thent
puposestoutzestemcestompove
unestnngofsesesnthemech-
nsms.ifwecncoectcesmpesfom
onoswhohvetheseseses,nhve
ccesstothemeces,twthene
possetostuythesesevthenuce
PupotentStem(PS)cesgenetefom
theptentsces.
TheCeptfompojectwstheefoe
setuppmytonustzePScesn
mkethemothoustnsfe.2010
eneustoensuethtoupojects
mtezeygvngthemthenecessy
esouces.TheCepojecteceve
funngcommtmentfomtheegon
uthotes,the Caisse des dpts et
consignations (bnkfoOfcdeposts)
nOSEO,pucentepsewhose
mstofunnsuppotnnovtonn
compnygowth.
inton,twonon-excusvecensegee-
mentshvegvenCeectswoweccess
tothenucePupotentStem(PS)ce
ptentpotfoosngfomthewokofPofessoShnyYmnkttheCentefo
PSCeresechnappcton(Cra)t
thenvestyofKyoto(Jpn).Ceectss
thestcompnynthewotoecense
yPSacemJpnunethsPSce
ptentpotfoofohumnthepeutcsn
pophyctcs.
Ceectshssoenteentocoo-
tonwthCratocomneCeectsgenome
engneengtechnoogeswthCrasPSce
technoogytompovethentuchcte-
stcsofPScesfousesceutoos.
Weesttng2011wththeessentkeysfosuccess.itsnowuptoustoensue
thtoupojectsechthefupotent.
d.dvSouve
egnucesesoffenewposstesfoestshngvetentvetoo.cogeectuythoughttoeveypomsngsouceoftentvetooevtve:fue,chemcmoecuesomtes.
Thssptyuetothestongtytopouceosnthegowthneffectveyete.
ocusonosusttuteOumegnucesetechnoogywoue
wesutetohepoptmzethesettsn
enennustztonofthepocesses.
asntusouce,geoneecuenty
thoughttoeetomeettheemn
fofueeffectveywthoutcompetngwth
nothenee,suchsfoosuppy.age
sousethepocessofphotosynthessn
theefoeneeght,wtenCO2,mkng
themnenvonmentyfenyfue.
itwsfotheseesonstht,n2010,
Ceectssetupesechtemtofocuson
mco-ge,ntomsnptcu,s
possesusttutefoo.dtomsey
pynmpotntoentheenvonment
ecusetheypouceone-fthofoxygen
pouceonetheveyye.dtoms
eunceugefounnmosthum
envonmentsnoffehugevesty,wth
100,000speceseyente.
apemnypogmwstheefoeset
uptCeectsnoetopovepoofof
pncpessoonsposse,ptcuy
egngthepotentofmegnucese
efcencynthesege.
COrPOraTEdEVElOPENT
d.dvSouve,EVPCopotedeveopment d.aetoamto,pojectee,genomeengneengonge
dffeentknsoftoms
iNTEllECTal PrOPErTY HaN rESOrCES CONiCaTiONS
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l Ceectsl actvtyrepot2010 l26 l Ceectsl actvtyrepot2010 l 27
S/Ca Euope as aust
2000
2001
2002
2003
2004
2005
2006
2007
2008
2009
2010
OneofCeectsfoemostssetsstsnteectupopetypotfoo.TheinsttutPsteuptentfmescovengtheuseofhomoogousecomntonnmegnucese-typeenonucesesfonucngdNaecomntonfometheptfomuponwhchCeectswsntyfoune.
Wehvesnceeveopestongpop-
etypotfoo,wthptentscossthee
octegoesgenepncpes,
megnucesesnthespeccpp-
ctons,nmnufctungpocessesof
moemegnuceses.Ousttegy
nvovesexpnngthenteectupopety
potfootofuthepotectoupoucts,
theppctons,nthecoesponng
oyoftechnoogcknowege.
attheenof2010,Ceectsownethe
ghtsto83ptentswowe,ncung35
nthenteSttesnCn.Thsmens
tht25newptentsweeotneovethe
couseoftheye.Ceectscuentyhs
moethn260ptentppctonspenng.
in2010,Ceectsoughtthessetsof
CytoPuseinc.,compnythtspeczes
neectopotonsystems,nthoughths
opetonotne16newptentsto
totsnteectupopetypotfoo.Thnks
tothesenewptents,Ceectsweceve
eguncome(>$600,000n2009),eve
fomthegntngofexpottoncenses
foCytoPusesHymuneTeectofuson
technoogy.
Thecompnystvestooptmzenupho
thenteectupopetytcensefomtheinsttutPsteu.Ceectssoenevosto
pomotetsnteectupopetyyefen-
ngtsownghts,wheespectngtheghts
ofothes.
Evolution of Cellectis IP portfolio
NumeofptentsgntetoCeects,peyengeogphce
100
80
60
40
20
0
iNTEllECTalPrOPErTY
Wthhecountththsgownfom5n2000to129ttheenof2010,thekeywonoucompnysclelyevelopment,ntemsofouemployeesnthetlents,swellsoucoetechnology.
ThepocytCeectsnvovesenngou
stfftoevovesthecompnyoesso.
reectveofthepssonnetemnton
toseeCeectsecomemjopyen
otechnoogy,someofthepevouspoject
eesenowttheheofeptment
nsomeotoytechncnshvetken
upengneengssstntfunctons.
oeove,Ceectspovessuppotfo
thosenvuswhowouketofuthe
theeuctonnengneeng.
Theseynmcshepusttctnewtent
toCeects,unfycuentstffnestsh
genunecompnyenttynthemst
ofcontnuousgowthneveopment.
Ceectsspouoftstemsptnntens
toexpntsenegytomntnt.
Temungssochevethough
semnsnotheegun-houseevents
thteognzeytheCommunctons
deptment.Theeptmentssoespon-
sefopomotngCeectstechnoogy
nexpetse.Oneofthemntoossetup
ytheCommunctonsdeptmentn
2010wsthenewcopotewestewth
sevekeyfetues:nfomtonshng,
ntectvtynectemee.
inghtoftsexpnson,Ceectschoseto
povevstostothewestewthcee
nmoeccessensghtntotswok,
pouctsnchevements.Vstostothe
newwesteeensueenhnceusty
nnfomtonshng,ncungmoe
stemneccesstonfomtonout
Ceectstechnoogesnpoucts.
ThenewCeectswestefocusesonthee
centthemes:
resechntechnoogy
busnesseveopment
investoetons
intontothesefocuses,theweste
offesnewfetuesnfeshcontentncung:
SevespeccrSSfees,sotht
suscescnmontotheCompnys
usnessnetme,choosngonythe
sujectsthtnteestthem.
infomtonshngvsocnetwoks
(ptcuyceooknTwtte).
downoeocumentsthtexpn
Ceectstechnoogy.
iusttveveosnegufetueson
vousspectsofCeectstechnoogy
tohepmpovethepucsunestnng
ofouscentcpogess.
HaNrESOrCESCONiCaTiONS
TheinteectuPopetyTem
SHarEHOldEr rElaTiONS
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lettestoshehoesitsveympotntfoCeectstofoge
cosenkswthtsshehoesnths
swhythelettetoShehoespoject
wsntten2010.Thssqutey
etteessesoeytoshehoestht
summzesncommentsonthetest
news.itoffeswyfoCeectsCEOan
Chouktomntnectnegu
contctnthusustngntust-
seetonshpetweenCeectsntsshehoes.EchlettetoShehoes
sohs3Questonsntevewwthone
ofthekeypeopetCeectsontopcof
ptcueevncetothecompnytthe
tmeofssue.Theessoocusonone
ofouconstnty-evovngctvtes.
Event:str&ddyOnNoveme30,2010,Ceectshets
stresech&deveopmentdy,towhch
tnvtenvestos,nystsntheme.
Thsevent,ntenetoethestnsees,
wschncefoCeectstofogestonge
teswththenvestmentcommunty,getto
knowthesenvusette,nexchnge
es.Theguestsgnemoen-epth
unestnngoftheusnessctvtes
ofCeectsntssuseswthtouof
thepemses,nptcutheotoes,
ungwhchtheymetthetemsththve
meCeectsthecompnytstoyn
epesentthevuestuphos.
oowngtouoftspopetytechnoogy
fctythtcetes,poucesntests
megnuceses,memesoftheCeects
mngementnscentctemsete
thectvteswthntscoeusnesses:
megnuceseengneengtechnoogy,
esechtoos,genomeengneengnpnts,
nthepeutcpogms.
dSegebun,fomtheenchuscu
dystophyassocton(a),gven
upteonhsognztonscooton
wthCeectsnthepeutcs.Toconcue
theevent,thepotentppctonsofnucepupotentstemce(PS)techno-
oges,fowhchCeectsecentysgne
censegeementwthPSacemJpn,
weesoesce.
Thepesenttonsevet
www.ceects.com/nvestos.
TheCeectsCommunctonstemso
mngeseveythngthtetestothe
puctonofyeynhf-yeynnc
esuts,nognzesthegenessemes
whchecheyChstnPoc,Chmnoftheboofdectos,n
tteneytheCeectsshehoes.
athenfomtonoutthegene
ssemessventheinvestose
ontheCeectswestewww.ceects.com.
ypostonntheiTgoupscenttotheusnessnnvoveswokngcoseywththestfftCeects.imgicnhepthempogesstheesech.Snlpze,NetwoknSystemTechncn
ichosetowoktCeectsecusetswe-known,gowngotechnncethtseveopngnnovtve
technoogeswththeojectveofecomngtheeengenomeengneeng.
JuenPontn,Senoltechncn
Ceectssynmccompnynwhchtemsptnstongmotvtonethevngfocesthteusfutheeveyynpusungnnovtvenfscntngmttes.Chsteerochon,resechenCeunoecuboogytEctyce
Ceectsshuofnnovtonnwhchcommunctonmongtemsnetweeneptmentsowsctvey.Wokngwthtemsthtehghyskenopensveyewngpesonnpofessonexpeencefome.JustnEyquem,TneeEngneengpepnghsthesstCeectsthepeutcs
SHarEHOldErrElaTiONS
Cellectis staff
4%
25%
71%
Scentcdeptments(Sd)busnessdeveopmentamnstton
Ceects71%SdCeectsoesech71%SdCeectsthepeutcs*100%SdCeectspntscences100%SdEctyce100%Sd
*exgenomesugey
bekownyctegoes
Breakdown by subsidiariesTot:129(ncung4ntheSan2ntheK)
EctyceTem
lotoestou
CEllECTiSbiOrESEarCH
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l Ceectsl actvtyrepot2010 l30 l Ceectsl actvtyrepot2010 l 31
SbSidiariES
clebozec,ChefnncOfceofCeectsnChefExecutveOfceofCeectsoesech
2010Hghghts2010wsfoCeectsoesechyeofconsotonntemsofesechneveop-
ment,suchthtweenowetomketove150poucts.Weeptcuypese
wththsgowth,gventhtweonyuncheoustktndeceme2008foowngthe
cetonofCeectsoesechnJuneofthtye.Weeetosuppygenume
ofnewpoucts,usttngveyveseoffeng.in2010,wehvepteoupouctsto
vetyofcenes,evenceoftheefcencyywhchwengnewpouctstomket.
Wesooptenewmketngsttegytonceseouvstynpycometo
compettonwththemngopyesnesech.Thsnvoveothttonvetsng
nthepessnonthewe,swesttenncetsome20teshows,utsomoe
ognmketngmethos:nntectveweptfommetthescentccommunty
enggenusngnewgenomcs-etetechnques 1ncompettontopovenncng
fopost-octopostonnvovngpojectncunggenomecustomzton.om
usnesspespectve,theesutsweeveyencougngsncewemoethnoueou
opetngevenues.WesontegtethemketsheofCytoPuse,namecncompny
whosessetswecque.Thsncuecompetengeofeectopotonnstuments
eycommecze,swestheetenteectupopety.Ceectsoesechs2010
opetngevenuesmetthetgetsnexpecttonsset.inthecuenteconomccmte,
thsssomethngwecneveypouof.Ousuccesssuetooutentetem,
whchnowncues20empoyees,two-thsnr&dnone-thnsesnmketng.
Chengesfo2011dungthecomngye,wewfocusontheemnestocheveougoofpovng
oucustomeswththousnsofpoucts.Thestestechnoogy.Wheeswecuenty
suppymnytgetentegtontoos,oumstosuppyothtgetemoctonn
nctvtonsoutonsso.Theseconestoexpnoupouctnenothespeces,such
spntskeArabidopsis thaliana,wey-usepntmoe,oothekeynmmoes,
suchsnemtoe C. elegans.in2011thesekeymoesetounegoNHEJ(NonHomo-
ogousEnJonng)megnuceseoTalEN(Tnscptonactvto-lkeEffectoNucese)
testng,oncethsnewtechnoogysfuympementeonouptfom.ThetheoffocuswetoexpnoueofppctonstotheadET(asopton,dstuton,eto-
sm,ExcetonnToxcty)testtosuppycenefocnceesech,cosetotheptents
physoogcety.oegeney,wemtomntnougowthntocheveshot-tem
pottywheweepoyesesfocenEuopenthenteSttes.itwswth
thsnmnthttheamecnsusyCeectsoesechinc.wssetupnOctoe
2010.Oumfo2011stosetuptemsnthenteSttesnEuope,toputusonn
equfootngwthoucompettos.Thenexttwoyesweecsvestheywetemne
thetypeofpottywecnexpecttocheve.
clebozec,CEOofCeectsoesech
1www.genome-engneeng.com
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l Ceectsl actvtyrepot2010 l32 l Ceectsl actvtyrepot2010 l 33
Ceectsoesechsuppesesechesnthefescenceswthesyney-to-usetoosfocetngcesnognsmsththvetheesepefomnce.
Ceectsoesecheveopsnmketsgenomecustomztonpouctsthtcneuse
tocetestensogenccenes,wthchctestcsnpefomncesptetothee
mnmkets:ugeveopment,genefunctonstuesnpotenpoucton.
cGPSncGPSCustomktsThesektsenesynefcentwyofusngCeectsmegnucesetechnooges.
Wththekts,genecnentegtentonextemeypecsestenthegenome.
attheenof2010,Ceectsoesechwssuppyngove150pouctsvtsonne
shopnntentonstutonnetwok(innCn).ThecompnyCeects
oesechinc.wsecentysetupnCmge,sschusetts(nteSttes)to
pomotethecompnyspouctsnceneengneengsevcesthoughoutamec.
Thegenomecustomztonktscontnegentsnpotocosthtenepecsen
tgetemoctonofdNasequencesnthegenomeofnteest,ofwhethecene,
pmyce,stemceonenteognsm.
Tgetentegtonnvovescopynpstepocessthtspefomey
megnucese,nntegtonmtxnthehomoogousecomntonpocess.
Ceectsoesechstgetentegtonktseesyney-to-use,nncue
competepotoco.Echktcontnsthecomponentsthteneeefotgete
ntegton:w-typeoengneeemegnucese,nntegtonmtx,n,n
cGPSktscene.
Theesechktsnenowncuesvenewcetypes,nthepouctoffengncues
piStoe,coectonofoun100ntegtonmtcesthtcneusewthffeent
kts.in2010,Ceectsoesechunchetsstcustomzesevcetoeveste
conesycGPStgetentegton.2010soswmjor&dptneshpwthlonz,
woeenotechnoogymnufctungwththemofmofynglonzspopety
ceneCHOK1SVusetopouceotechnoogcugs.
astuyshowethtCeectsoesechstechnoogyseonmegnucese-ven
tgetentegtonsfste,moeeenmoeefcentthnttonmethosn
evngce-sessysfoHghThoughputSceenng(HTS)stues,whchowp
sceenngofvstnume
ofnewmoecues.egnucese-ventgetentegtonusngCeects
oesechscGPSCHO-K1ktstheefoehghyefcentfougeveopment.
Theesutsofthsscentcstuyfocusngonunquemethofogenetngstece
nescomptewthhghthoughputsceenngweepusheonneJuy12,2010ythe
JounofbomoecuSceenng.
Thechengestoepcettonceengneengmethosseonnommoctonwthtgetentegton.clebozec
Meganuclease-Driven Targeted Integrationin CHO-K1 Cells for the Fast Generation ofHTS-Compatible Cell-Based AssaysCnosJ,OuvyC,lmmyV,eyi,Cpet.l,ouhtN,GuennS.P,beutS,NosjenO,eyG,devvyS,JcqmcqC,leuhoteC,thsl,deenC,boutnJ.a,duchteuP,
Cog,nPquesJ. Biomol. Screen.Sept2010-vo15no8p.956-967
integtonofntuegnuceseecogntonste
Wtypeegnucese
Engneeeegnucese
dNarep dNarep
egnuceseengneeng
cGPS cGPS
CustomcGPS cGPS
Custom
CEllECTiSPlaNTSCiENCES
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betweennowntheye2050,thewospoputonwgowfomppoxmtey6ontomoethn9onpeope.Thsncesenpoputonsze,coupewthchngngcmtenncesngymteesoucesfogcutue,wpceenomousstnsonoupnetscoectvetytopovethefoo,fuenenecessytosustnhumnkn.
Oneghtspotonthsothewsetouesomescenosthepomseofnewtechnooges.
Genomeengneeng,nptcu,wpovenewcoppntswthttsthtcnmeet
theemnspceongcutuenthecomngeces.
Cetench2010,Ceectspntscencesssusyectetoppyng
sequence-speccnucesesfopntstoeveopnewspeceswthttsofvue.The
compnysmngosetoncesencceeteuseofCeectspopetytechno-
ogyngcutuotechnoogy,tooenthecompnysexpetsetottctnewn
expnecensngoppotuntes,ntoexpoetheeveopmentofpopetytts.
Ceectspntscencessecteywo-cssscentctem.Thecompnysscentsts
weethesttompementgenomeengneengnpnts.amongthetemmemess
thesusyChefScentcOfce,dneVoyts,whosntentonyenownefo
hswokonpntgenomeengneeng.d.Voytsssopofessotthenvestyof
nnesotwheehehestheCentefoGenomeEngneeng.d.engZhngseves
sthesusydectoofresech,nhehsextensveexpeencenmze,cen
Arabidopsisgenomemocton.inton,thecompnysstffeytwoPhd-eve
scentstsntwoesechssoctes.
intsstyeofopeton,Ceectspntscencesemonstteefcentmocton
ofpntgenesyengneeemegnuceses.lucths,Pogmsnbusnessdeve-
opmentdecto,hghghtethtwthnmonthsoftsncepton,Ceectspntscences
otnepoof-of-conceptt.Theseesutsemonsttethtmegnuceseshvesm
cpctesnpntcesspevousyshownnhumnces.omthsstongt,Ceects
pntscencessnowfocusngontscommecms:toevepoucts,sevcesn
censesfothegcutuenustyswestoupotfooofgcututtsnts
eofexpetsetgetemutgenessnseectepntspeces.
Thssveyexctngtmenpntoogy,nouecentvncesngenomeengneengmknewe.itsstmutngtoenvovencompnythtcnhvesuchectmpctonhumnvesnthewe-engofthepnet.P.dneVoyts
Thene-temgoofCeectspntscencesstomovewyfommoepnts(Arabi-
dopsisntocco)ntoppytstechnoogytocommecpntspeces.TheCeects
pntscencestemeyhsexpetsentnsfomngcon,censoyen,nthese
sksweessentnepoyngthenextphseofthecompny.
ithstoetkenntoccountthttheegutoypocessequetoeesegenetcy
moepntsntotheenvonmentsveyexpensve.asCeectspntscencesscentc
temmpementstsgenomeengneengstteges,theyensuethtonymnm
chngesemetothepntsgenome.itshopethtpntgenomesmoewth
thsknofpecsonweettecceptenthemketpce.
PofessoVoytshseenwthCeectspntscencessChefScentcOfcesnce
thecompnywssetup.HespofessonthedeptmentofGenetcs,Ceboogy
ndeveopment,ndectooftheCentefoGenomeEngneengtthenvesty
ofnnesotnnnepos.Speczngnmoecuoogyngenetcs,P.Voyts
esechfocusesonpntgenomemoctonusngnucesesthtcnecognze
speccdNasequences.
HesneecteeowoftheamecnassoctonfotheavncementofScence.
HesnassocteEtofothejoun GeneticsnEto-n-Cheffothejoun
Mobile DNA.Hestheco-founeoftheZncngeConsotum,goupofscentsts
ectetopomotngppctonsofzncngepotensfogenomemocton.
Hehsuthoeoco-uthoeozensofscentcppesntheeofgenome
engneengnpntspeces.
P.dneVoyts,ChefScentcOfceofCeectspntscences
ECTYCEll
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l Ceectsl actvtyrepot2010 l36 l Ceectsl actvtyrepot2010 l 37
EctycewsestshenSepteme2009toppytheenetsofCeectscoetechnoogestoeveopmentswthntheeofstemces,especynucepupotentstem(PS)ces.
Thssusywutzethesetechnoogestotnsfomstemcesntotueeveop-
mentnmnufctungtoosthtwenove,oust,epoucenusenhgh
thoughputexpements.
Ectycehsnumeofesechnttvesunewythttkevntgeoftheenets
ofmegnucesesfogenomcengneeng.Thesepogmsncue:
deveopmentofCenPSCesygenetngPScesfomutcesusngtgete
ntegtonnexcsonofepogmmngfctos,whchwccumventthenecesstyto
usenomvntegtonmethosfoPScepoucton.
deveopmentofnovenepoucemethosfoceuffeenttonfomPSces
usngtgetegenomeengneengtechnooges.
deveopmentofoustmethooogesnpocessesfogescepouctonofPS
nffeentteces
CetonofPSseceesthtweeysutetohghthoughputug
sceenngppctons.
inOctoe,Ceectsn-censenucePupotentStemCeTechnoogyfomPSacem
Jpn.Thetwoseptenon-excusvegeementsgntCeectswoweccess
tothenucePupotentStem(PS)cesptentpotfoosngfomthewokof
PofessoShnyYmnkttheCentefoPSCeresechnappcton(Cra)ofthenvestyofKyoto,Jpn.
PofessoYmnknhscoeguesponeeethsgounekngwoknCeects
sthestcompnywowetoecenseyPSacemJpnunethsPSce
ptentpotfoofohumnthepeutcsnpophyctcs.
Ectycewsfouneonthefctthteveopngey-stgehghthoughputsceenng
ssysthtettepectmoecueseffectontheognsmswhoeoongenet-
cyvesepoputoncousgncntyeucethehghtttontecuentyoseve
ungugeveopment.avncesuneeveopmenttEctycemysoopennew
oppotuntesnthepeutcs,ncungegenetvemecne.
WhtsnnucePupotentStemce?annucepupotentStemce(oPSce)snutceththseenepogmme
toehvekenemyoncStemce(oESce).
Whennovumsfetze,theeggves,nwthnshotspceoftmecese
fomeththvethepotenttoeveopntothetssuesoftheoy.Theseceseceemyoncstemcesneknownspupotentces.Pupotencysthety
togeneteffeentcetypes.
astheymtue,EScesguyosetheffeenttonpotentneveop
functonspeczton.oexmpe,ymphocytewtckenfectons,whees
pncetccewecpeofpoucngnsunfotheestoftsfe.
ntecenty,twseevetoempossefocetoetuntostteofmmtuty
necovetsffeenttonpotent.in2006,thetemofPofessoShnyYmnk
tKyotonvestyemonsttethttspossetoepogmmtueutcetogve
tckthepopetesofnemyoncstemce.inoefothsutcetoetunto
stteofmmtutyneetoffeenttentocetypes,youjustneettoexpess
fougenes(notnomyexpessenutces).Thesecesetemenucepupo-
tentstemces,oPSces.
EctycesesponsefospengCeectsgenomeengneengtechnoogesontothestemceenoetotnsfomthesecesntotuemnufctungtoosthteoust,epoucen
usenhghthoughputexpements.dvSouve,CEOofEctyce
How to make an iPS
4genes
pecseyequecetypeske...
...Neveces ...booces ...Ccces
Pupotentstemces
autce
CEllECTiSTHEraPETiCS
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l Ceectsl actvtyrepot2010 l38 l Ceectsl actvtyrepot2010 l 39
Ceectsthepeutcs,unchen2008unethenmeCeectsgenomesugey,focusesontheeveopmentofnnovtvethepeutcppochesthtusemegnucesestotetgenetcseses(suchsmuscuystophesnhemophs),cncesnpesstentvnfectons(hepesketts,aidS).
Thssusyhstemof19,ncung10Phds,nenevostonnnovtve
tetmentstotetptentswthseouscontonsthteesstnttoconventon
thepy.Ceectsthepeutcsmstofuunmetmecnees.Thetetment
pgmnvovestgetngthedNasequencethtcusesthesese,whchmyennte(genetcsese)ocque(vnfecton,cnces).
Thegenomesugeyppochmstoghtthecuseofptcusesencue
theptent,thethnmeeytetsymptoms.Sncetsncepton,Ceectsthepeutcs
hswoketogethewthtsptnescemcesechgoupsncncnsove
thewo(ncungChenHosptboston,insttuteVson,GenomcVson,CNrS,
iNSEr,nmnyothes)totnsfomtsesechntoeffectvemectetments.
CuentcnctsafteCeectsuyoutofthessetsofCytoPusenSepteme2010,thecompnyece
topusuetheptneshppogmththeensetupyCytoPusefotheuseofeecto-
potosfovccnton.CytoPuseseectopotontechnoogysptcuyeffectvefo
ntegtngmoecuessuchsmegnucesesntonyknofce.
Ceectssuppesthedemvxntemeectopotonequpmenttootoes
cyngoutcnctsequngthsknofequpment.demvxssystemtht
ppesneectcetofcttethentegtonofsmfgmentsofdNantoces,
theeypotentzngtheeffectofdNavccnesystmutngthemmuneesponse.
athoughdemvxwsognyeveopefocncevccnetetments,tsso
engtestetoyspophyctcppochonHiVnfectons.Theecnctse
engconuctettheKonskinsttutenttheCnceCentenStockhom,
ntheotoesheeyPofessosbttWhennEcSnstm,swest
ppsnvestyyPofessoTottemnndoctoYchnn.
2010wsyeofntensveenngfooththeCeectsthepeutcstemsntheptnesnognztonsnthemecotechnoogyewthwhomwecoote.aongsethestpoofsof
concept,weoftenponeeetechnoogyuse,pouctonnvton.Syvnanou,Phd,pecncpojecteetCeectsthepeutcs
2010wstunngpontfoCeectsthepeutcs,whchotneveypomsngesuts,ptcuyntemsofpoofofconceptonthentvctvtyofmegnuceses*.dungtheye,wesomesgncntpogessnoucethepypogms.
inee,outemhseenetoentfygootgetsfotheso-ceSfeHo
ppoch.Thsppochconsstsnenngstengenomethtowsthensetonof
newgene,whchmghteexpessewthoutntefengwththecefunctons.
Wesompementeneffectvenon-vvectoztonppochusngtheeecto-
potonsystemotnefomthetkeoveofCytoPusesssets.inton,we
otneveygoopemnyesutsnhomoogousecomntonnpmyces,
whchpepethegounfooucethepyeveopment.
ingenethepy,theesutsotneyouCnnptnesonduchennemuscu
ystophy*offesgooexmpeoftheenegyputnouthepeutcesechon
megnucesesyoucemcptnes.
inconcuson,ouwokn2010eneustootnpoofsofconceptnconm
ouscentcnegutoysttegy.Westt2011wththemofotnngpoofsof
pncpefotheseesutsonkeypthoogessuchshemoph*.
d.Coedesseux
*moenfomtonnextpge
egnucesescnesovethessueofnomntegton.ThepecsonoftheCeectstechnoogymensthtthenewgenecneeveetpeetemnensfeocton.Wehvehghhopesthtoneythsesechwetoeffectvenfe-enhncngthepesfohemoph
ptents.d.TheyVnendesschend.neeChuh,VibVesusresechCentettheCthocnvestyofleuven(begum)
d.Coedesseux,dectoofopetonst
Ceectsthepeutcs
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iNaNCialSTaTEENTS
HepesvusScentstsfomCeectsthepeutcs,theenchNtonCentefoecresech
(CNrS),ninsttuteVson(Ps)hveuseCeectspopetymegnucesesto
successfuypeventnfectonofcutuecesyhepesvus(HSV-1).Theesechws
pushenthejounMolecular Therapy.Theseethestpoofofconcepttsto
showthtmegnucesescnpeventvnfecton.Thetceesceshowscentsts
usespeccmegnucesesengneeeyCeectstopeventthenfectonofhumn
cutuecesyHSV-1.
SusequentnyssofthetetecesysequencngshowethtthevdNah
successfuyeencppeythemegnucese,ntheefoeosttscpctytoepcte
nspe.
duchennemuscuystophyresechestthelvnvestyHosptCentenQueec(Cn)usemeg-
nucesesengneeeyCeectstoestoetheexpessonofmcoystophnnhumn
myostsin vitronnhumnces in vivo.Theyweetheefoeetoshowthe
potentofusngmegnucesestotetduchennemuscuystophy.Thesenew
nngsweepushenthescentcjounGene Therapy.
GenethepyfotetnghemophThefescencesesechnsttuteVibnCeectsecootngonesechntonew
ppochestotethemoph.Theyeusngmegnucesestoepcefutyoo
cottngfctogeneswthfunctoncopes.Hemophsgoupofheety,ecessve,
X-nkegenetcsoesthtmptheoystytocontooocottngo
coguton.Hemophptentssuffetovousegeesfomuncontoenteno
exteneengfoovessesoken.inessuchsthenonsejonts,
thseengcneftopemnentyettng.
Publications
HepesvusMeganuclease-mediated Inhibition ofHSV1 Infection in Cultured CellsGosseS,HuotN,hetC,anouS,beuS,Ceedl,Chon-Sotnei,JcqmcqC,Chpeeb,Egna,desseuxC,Cone,ConseeE,Pques,letoue,SmthJ.Molecular Therapy.2011Jn11.
duchennemuscuystophyMeganucleases can restore the readingframe of a mutated dystrophinChpeneP,PchvntC,rousseuJ,PquesnTemyJ.PGene Therapy (2010)17,846858;
iNaNCialSTaTEENTS
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l Ceectsl actvtyrepot2010 l42 l Ceectsl actvtyrepot2010 l 43
assets Equtynltes
g u es n t ho us n s o f eu o s d ec em e 3 1 201 0 de ce m e 3 1 20 09
Net intangible assets 3976 903
Net tangible assets 3913 2893
Non-current nancial assets 231 231
Deferred tax assets 8169 5133
Total non-current assets 16289 9160
inventoes 153 118
Teeceves 2491 1768
resechtxcetnsuses 6588 1570
Othecuentssets 2486 2465
Cshncshequvents 24048 45080
Total current assets 35766 51001
TOTAL ASSETS 52055 60161
g u es n t ho us n s o f eu o s de ce m e 3 1 20 10 d ec em e 3 1 200 9
Share capital 584 582
Shepemumneseves 45170 52404
Netncome/oss (8063) (7768)
Equity 37691 45218
retementenetogton 120 42
boowngs 809 491
Othennctes 588 795
Povsons 50 71
Total non current liabilities 1567 1399
boowngs 560 748
Othennctes 257 107
Teccountspye 8464 6548
defeencome 3516 6141
Total current liabilities 12797 13544
TOTAL EQUITY AND LIABILITIES 52055 60161
intngessetstdeceme31,2010ncue 2.4ofeveopmentexpensesn
1.5monfoptentscense.resechneveopmentexpensesconcenngktsn
pntctvtesencueunessetsfothesttmen2010,whchffesfom
scye2009.Thssesutofthegowthofothctvteswhchnowgeneteecu-
entevenues.Thetotofptentssetssgncntyncesesesutofthecqustonof
CytoPuse,inc.ssets.
Note:
Snce2010,thecompnyhsenevoetompovethequtyoftsnncepotngypushngcompeteccountsnnewthirSstns.
Thspocesssnongongone,equngfutheesttements,ptcuythoseconcenngevenueecognton,esngcontcts,efeetxssetsnthecptztonofcetneveopmentexpenses.TheguespesenteheetheefoencuetheesttementsequeyirSstns,uttthetmeofpushngthsepot,theconsoteccountsweestengeveweythesttutoyutos.
Tngessetsmnyconssteofotoyequpment.
ThetxcetssoctewthossescefowyCeectsntssusesws
cptze,gventhepospectsfoecovety.
The(cuentnnon-cuent)boowngstemmnycoesponetotheesttementof
esngcontctsfootoyequpment.
Theothennctesconssteofefunevncesgntetothegoupfo
vousesechpogms.insummy,thecompnynncetsneggewheequty
cpteche38ttheenof2010.
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l Ceectsl actvtyrepot2010 l44 l Ceectsl actvtyrepot2010 l 45
CshowSttementPot&loss
guesnthousnsofeuos
2010
12months
2009
12months
Net income/loss of the year (8063) (7768)
depecton&motzton 1186 793
Cptgnonssetsspos (61)
defeeTxes (3036) (1749)
Shesepyments 435 1045
Othenoncshtems 144 (30)
Cash Flow f rom operat ing activit ies before Working Capital change (9334) (7770)
( in ce se) / dec e se n c cou nt s ec e v e ( 75 8) ( 89 3)
(incese)/deceseofresechTxCet&Suses (5018) 1321
( dec e se ) /in ce se of t e c co un ts p y e 1 9 16 71
Net c hn ge no th e s set s n t es ( 26 72) 39 52
Change in working capital (6532) (4451)
NET CASH PR OVI DED (USED) BY OPER ATIN G ACTI VI TI ES (15866) (3319)
acqustonofntngessets (3528) (1017)
acqustonoftngessets (1477) (448)
acqustonofnncssets
Netncomefomseoftngessets 729 719
NET CASH PROVIDED (USED) BY INVESTING ACTIVITIES (4276) (746)
Newoowngs 0 0
repymentofong-temoowngs (983) (1079)
Poceesfomssunceofcommonstock 9 3 2 229 6
NET CASH PROVIDED (USED) BY FINANCING ACTIVITIES (890) 21217
CHANGE IN CASH AND CASH EQUIVALENTS (21032) 17152
Openngcshncshequvents 45080 27928
Cosngcshncshequvents 24048 45080
INCRE AS E (DECRE AS E) IN CAS H AND CASH EQUIVAL ENTS (21032) 17152
guesnthousnsofeuos
2010
12months
2009
12months
Ses 8166 7613
Otheevenues(resechtxcet&suses)1 7609 2527
Total operating revenues 15 775 10 140
CostofSes(censng-n) (1445) (1449)
Gross Margin Prot 14 330 8 691
resechndeveopment (12311) (7971)
ketngnamnstton (13457) (10601)
Othencome 0 78
Otheexpenses (27)
Current operating income/loss (11 465) (9 803)
nncncome 485 463
nncexpenses (153) (173)
Othenncncome&expenses 34 (4)
Net operating income/loss 366 286
incometx 3036 1749
NET INCOME (8 063) (7 768)
1incungesechtxcet 6148 1255
Thencesenther&dtxcetn2010esultenconseblencesenthsecevble
tdecembe31.intngblenvestmentsn2010conssteof 1.9cptlzeevelopment
expenses,longwthptentcqustons.appoxmtelyonethoftheyescshbun
wsffectebytempoyunfvoblevtonnwokngcptlequements.in2011,
thechngencsh(ncshequvlents)sexpectetobenethelevelofnetncome.
revenuesfomcensesnesechktsesnceseymoethn7%n2010.Ths
ncesessgncntconsengcetngeementswthmjogoupsththveeenpce
onhoonthemstofeognzton,ptcuyungtheunchpeoofnew
esechkts.Thenextscyesexpectetoshowfuthepogess,ptcuynktses
netesevces.
Ther&dtxcetncesesgncntyn2010( 6.1compewth1.3n2009),
mnyuetothencesenr&dexpentue(+54%)ntheeuctonngntseceve
n2010.ackgounstuywsconucteyCeectswththehepofconsutntsnoe
toestmtether&dtxcet.
Thencesenopetngexpensesn2010suetothempementtonofmjogowth
sttegyoutnen2009,theconsotonofupstemesechtemsfoCeects,n
ppctonptfomeveopmentfoechofthesuses.resechneveopment
expensesnceseyppoxmtey50%,whchemnsconsstentwththestffncese
(+50%fomtheenof2009totheenof2010).
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l Ceectsl actvtyrepot2010 l46 l Ceectsl actvtyrepot2010 l 47
ShePcenCptStuctue
aPPENdiCES
3
4
5
1
6
21.eeot62%
2.CopotePtnes4%
3.ounes&ngement15%
4.busnessange10%
5.VentueCpts5%
6.insttutPsteu4%
Shareholders Breakdown as of December 31, 2010
Ceectsshepceswowntunungthe2010nncye.infct,thest
tngyof2010nshetpceof10.95peshenthesty(deceme
31st)nshetpceof7.40peshe,thusshowngnetfof32.4%.Seve
fctosexpnthsowntun:thecontnueextofventuecptstsshstoc
shehoes,owytevoumesnmketvottynketosgncntmco-
economcuncetnty.
Thecptstuctuehscontnuetoevoveytheextofventuecptshehoes
nchngesesutngfomthecptncesettheenof2009.Thefeeot
hsnowechemoethn60%utthehcoehong,centeeounthetwofounes,theinsttutPsteu,theusnessngepesentnthecptsnce2000
nthenustcompnesthtenteen2008(regeneon)n2009(onsnto),
emnsunchnge.
Share Price Evolution
ShePcen SheVoumenthousnsofshes
100monmketcptzton.aout10,000she
vegevoume.
NYSE-EuonextaClS.Pa
2010
2009 ap Ju Oct
12
11
10
9
8
7
6
40
20
0
60
80
100
GOVErNaNCE
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l Ceectsl actvtyrepot2010 l48 l Ceectsl actvtyrepot2010 l 49
ExecutveCommtee,fomefttoght:dvSouve,anChouk,cPques,
dkPoet,Syvedessus,clebozec
ExecutveCommttee TheExecutveCommtteescomposeoftheseno-evemngementofCeects,whch
estshesconsoteojectves,montosthesttegcpojectsnecesonpotes.
itsmemesmpementthecompnyssttegynecttsy-to-yopetons.The
CommtteencuesanChoukndvSouve,thecompnysco-founes.
anChouk,Phd, Chie Executive Ofcer
anChouk,Phd,founeofCeectsSa,hssevesChefExecutveOfcesnce
thecompnysncepton.d.Chouksponeenthenyssnuseofmegnuceses
tomofycompexgenomes.Hehsmemnyofthectcosevtonsnthee
nsnuthoofthemostsgncntptentsontheuseofmegnucesesnvvo. d.ChoukecevePhdnoecuVoogyfomnvestyofPsVi/insttut
Psteu.d.Choukhsmepost-octostuesttheHvecSchoowthn
theeptmentofoecuecneofbostonsChensHospt.
dvSouve,Phd,Executive Vice President, Corporate Development
dvSouve,Phd,co-founeCeectsSawthdChouk,nhsseves
dectosnce2000.Potonceptonofthecompny,dSouvewstheheof
botechnoogylotoyttheCenteEtuesubouchet(enchnstyofdefense).
atthesmetme,dSouvewshengesechgoupnimmunoogytthePsteu
insttute.d.SouvetteneEcoePoytechnquenecevePhdnoecu
VoogyfomnvestyofPsVii/insttutPsteu.dSouvethenppe
ecomntontckngtechnoogestont-vmmunememoy,ttheEmoynvesty
VccneCentenatnt.
cPques,Phd, Chie Scientifc Ofcer
clebozec,Chie Financial Ofcer
dkPoet,Phd,Chie Business Ofcer
Syvedessus,Phd,Senior Vice President, Corporate Communication
boofdectosTheboofdectosetemnesthe
compnysttegynoveseesCeects
ctvtes.emesoftheboof
dectossevefotemoftheeyes;
theChmnconvenesmeetngs
whentseemenecessyoese.
Ondeceme31,2010:
ChstnPoc,Phd
Chairman, co-ounder o BiotechDeveloppement Conseils
tnbtsch,d
Independent director, Consultant
anChouk,Phd
Director, Chie Executive Ofcer, Cellectis
anGo
Independent director, Former Head o
Aventis Cropscience board o directors,
Consultant
rogeJ.Hjj,d
Director, Kaminvest Holding Representative
rchC.ugn,Phd
Independent director, Mallinckrodt Proes-
sor o Genetics at Harvard Medical School
and Director o the Harvard Gene Therapy
Initiative
dvSouve,Phd
Director, Executive Vice President,
Corporate Development, Cellectis
Psceate
Observer, Institut Pasteur Representative
ScentcavsoyboTheScentcavsoybo,setupn
2002,scomposeofeghtctvememes,
ppontefooneyenmeetstwce
ye.itsmssonstooutneCeectso
scentcoenttons.itpesentsCeects
mngementtemwthmethosn
sttegestochevethecompnystechno-
ogcgos.itevutesthewokcheve
ungtheyentheesutsotne.
Ondeceme31,2010:
Pof.nosJco,HonoyChmn,Phd
Collge de France, Paris, France
Pof.roneyJ.rothsten,Phd,Chmn
Columbia University, New York, USA
Pof.eeckW.at,Phd
Howard Hughes Medical Institute,
Chevy Chase, USA; Harvard
Medical School, Boston, USA
Pof.bendujon,Phd
Universit Pierre et Marie Curie (Paris VI) -
Institut Pasteur, Paris, France
Pof.ansche,d,Phd
Hpital Necker - Enants Malades, Paris,
France
Pof.JmesE.He,Phd
Brandeis University, Waltham, USA
Pof.densPompon,Phd
CenteofoecuGenetcs,
Gf-su-Yvette,nce
Pof.Jos-anShe,d,Phd
Hpital des Quinze-Vingts, Paris, France
Pof.lusSeno,Phd
Center for Genomic Regulation,
Barcelona, Spain
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Ceects
Contct:Ceects102venueGstonrousse93230romnvenceTe:+33(0)[email protected]
Photos:rmontneznckbeonceCeectsTmrummehoff
EceeiNraChstopheteCcPochezStockphoto
Gphcdesgn:VentnHemnn
Pntng:GphCente
PnteonSCceteecyceppeusngvegetesenks.
dscmeThspuctonnthenfomtoncontneheenonotconsttutenoffetoseosusce,osocttonofnoffetouyosusce,foshesnCeectsnnycounty.Thspuctoncontnsfow-ookngsttementsthtetetotheCompnysojectvesseonthecuentexpecttonsnssumptonsoftheCompnysmngementonynnvoveunfoeseeesknuncetntesthtcoucusetheCompnytoftochevetheojectvesexpesseythefow-ookngsttementsove.
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