(classical) genetics
DESCRIPTION
(classical) genetics. causality. DNA. phenotype. ‘reverse’ genetics. T. C. The ‘gapped duplex’ method of in vitro mutagenesis. Gapped duplex method with phenotypic selection. Target : replace Trp by Phe Screening aid : Mnl I cleavage Mismatch primer : …GCCCT G GGCT TC GGTGGCA…. - PowerPoint PPT PresentationTRANSCRIPT
(classical) genetics
causalityphenotypeDNA
‘reverse’ genetics
TC
The ‘gapped duplex’ method of in vitro mutagenesis
Gapped duplex method with phenotypic selection
Creating or removing a restriction recognition site as an aid in screening for site-specific mutants
Target : replace Trp by Phe
Screening aid : MnlI cleavage
Mismatch primer : …GCCCTGGGCTTCGGTGGCA…
Codewoord tabel voor ‘reverse translation’
Leu = 1T2 Arg = 3G4 Ser = 567