Citometrie in flux

FCM Flow Cytometry

2/24/2012

Citometrie in flux - utilizari Dimensionarea particulelor (celule, componente

celulare) Numararea celulelor Determinarea viabilitatii celulelor Determinarea continutului total de ARN si ADN Determinarea continutului de clorofila Determinarea unor activitati enzimatice Evidentierea modificarilor proteinelor Evidentierea modificarilor genetice

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Structura unui citometru pentru citometrie in flux Sistem pentru circulatia fluidului Sistem optic

- Sursa de lumina (Laser, Lampi Hg sau Xe) - Filtre Sistem de detectie (ADC) Sistem electronic Calculator

2/24/2012

Schema unui citometru

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Focalizare hidrodinamicaThe image cannot be displayed. Your computer may not have enough memory to open the image, or the image may have been corrupted. Restart your computer, and then open the file again. If the red x still appears, you may have to delete the image and then insert it again.

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Interogarea particulelor

Signal

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Tipuri de filtre optice

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Sortare electrostatica in flux

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Spectrul luminii

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Principiul fluorescentei. Shift Stokes

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Absorbanta si emisia luminii de catre fluoroisotiocianat (FITC)

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Coloranti singulari

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Coloranti pereche

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Compensarea fluorescentei

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